Antimicrobial Properties of Aloe Vera Gel Extracts against Bacterial Isolates from Wound of Donkey
Antimicrobial Properties of Aloe Vera Gel Extracts against Bacterial Isolates from Wound of Donkey
Safia Arbab1, Rehana Buriro1, Shams Uddin Bhugio1, Atta Hussain Shah2, Jamila Soomro3,Qudratullah Kalwar4, Saqib Ali Fazilani1 and Waseem Ali Vistro5*
ABSTRACT
Despite the exploration of antibacterial activity of the Aloe Vera in different families of animals. However, very limited information is documented about antibacterial activity of Aloe Vera in equines. The present study was designed to check the antibacterial activity of the Aloe Vera against most pathogens found in the wounds of donkey. For this purpose, two different concentrations 30 µl and 60 µl crude as well as ethanol extract of Aloe Vera were used. During this experiment, total 50 wound samples were collected from donkey wounds. All 50 samples were found contaminated with different bacterial organism, among all those 45, 35, 33, and 25 were recorded positive for S. aureus, E. coli, Shigella and Salmonella spp respectively. However, out of 50 sample, 34 (68%) were found to have different bacterial species and 16 (32%) were recorded with pure contamination. At 30 µl of crude Aloe Vera isolated bacterial organisms did not show any susceptibility, whereas at 60 µl, organisms showed quit sensitive reactions. In contrast, ethanol extract of Aloe Vera showed better result as compared to crude Aloe Vera at both concentration i-e 30 µl and 60 µl. Among isolates, S. aureus showed high sensitivity (15-22 mm), followed by E. coli (14-18 mm), Shigella (13-15) and Salmonella spp (11-14 mm) both concentrations. The results of this study revealed that ethanol extract of Aloe Vera showed better antibacterial activity against all isolates as compared to pure Aloe Vera extract at both concentrations used, due to its high polarity.
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December 2020
Vol. 52, Iss. 6, Pages 2027-2426
