Comparison of ELISA and Serum Neutralization Test for Detection of Age-Specific Foot and Mouth Disease Virus Antibodies
Comparison of ELISA and Serum Neutralization Test for Detection of Age-Specific Foot and Mouth Disease Virus Antibodies
Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*
ABSTRACT
The present study was performed to compare the efficacy of two techniques i.e. serum neutralization test (SNT) and solid-phase competitive ELISA (SPCE) for foot and mouth disease virus (FMDV) structural antibodies detection in terms of their sensitivity and specificity. These methods were performed by using set of sera collected from cattle with foot-and-mouth disease vaccination. The pattern of antibody titers in animals of different age groups was observed as less than 1 year, 1-2 years and more than 2 years for both ELISA and SNT. The peak immune response measured by SNT was 2.8log10 whereas ELISA detected serum antibody titer up to 2.4 log10. Findings showed that highest titers were seen in animals of age > 2 years i.e. 1.70 log10 (for serotype Asia 1), 1.99 log10 (for serotype A) and 1.53 log10 (for serotype O) in ELISA while in SNT, antibody titers were found to be 1.86 log10 (for serotype Asia 1), 2.26 log10 (for serotype A), 2.11 log10 (for serotype O) respectively. Therefore, it was concluded that SNT is more suitable than SP-ELISA as titration method for the detection of antibodies against FMDV structural proteins.
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