This study was designed to compare the cytological changes in chicken infectious anemia virus (CIAV) infected bone marrow in broiler chicken. Bone marrow samples were collected from proximal tibiotarsus and femur of each of the five healthy and CAV-infected flocks under natural condition. The bone marrow smears were fixed, stained with Romanowsky Giemsa Stain (RGS) and examined under microscope. Results showed a significant difference between the cellularity of normal and CAV-infected birds. While comparing healthy and infected chicken bone marrow, we observed a decreased mean erythroid cell percentage (39.95 versus 17.95%), myeloid cell percentage (57.29 versus 21.98%) and the mean M/E ratio (1.43 versus 1.22), respectively. Bone marrow smears were normocellular among healthy birds while, in diseased chicken, severe aplasia was noted. The bone marrow findings suggested an easy, quick and cost-effective approach for supportive diagnosis of chicken anemia virus infection and therefore could be replicated for efficient disease diagnostics in resource-limited setting.
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