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Extended-Spectrum-β-Lactamase Producing Multidrug Resistant Klebsiella pneumoniae Isolates from Pediatrics

Extended-Spectrum-β-Lactamase Producing Multidrug Resistant Klebsiella pneumoniae Isolates from Pediatrics

Sumreen Hayat1,6, Muhammad Hussnain Siddique2, Bilal Aslam1, Habibullah Nadeem2, Asma Ashraf3, Muhammad Saqalein1, Mohsin Khurshid4, Naveed Shahzad5 and Saima Muzammil1,*

1Department of Microbiology, Government College University, Faisalabad
2Department of Bioinformatics and Biotechnology, Government College University, Faisalabad
3Department of Zoology, Government College University, Faisalabad
4College of Allied health Professionals, Directorate of Medical Sciences, Government College University, Faisalabad
5School of Biological Sciences, University of the Punjab, Lahore
6Department of Biotechnology, University of Sargodha, University Road, Sargodha

Sumreen Hayat and M Hussnain Siddique have contributed equally to this article.

*    Corresponding author:



Klebsiella pneumoniae is mainly responsible for nosocomial and urinary tract infections (UTIs) in pediatric and adult population. The ESBL producing K. pneumoniae present an alarming situation for clinicians by limiting the treatment options. We aimed to determine the antibiotic resistance pattern and characterization of ESBL producing K. pneumoniae isolated from children of different age groups. K. pneumoniae isolated from blood and urine samples were identified through API® 20E kit. Antibiogram was determined by disc diffusion method and isolates were screened for ESBL production using double-disc synergy test (DDST). Molecular detection for the presence of bla-SHV and bla-TEM was carried out by polymerase chain reaction (PCR). Of 392 blood and urine samples, 120 were found positive for microbial growth. Antibiotic susceptibility testing of K. pneumoniae isolates illustrated high resistance to ceftazidime (95.6%). Phenotypically,52.17% of K. pneumoniae isolates were found to be ESBL producers as observed by double disc synergy test while the tested ESBL genes were detected in 56.52% of isolates. The studyrevealed multidrug resistance phenotypes among K. pneumoniae isolates with higher prevalence of ESBL producing Klebsiella pneumoniae among the children with urinary tract infections. Based on our findings, it is recommended that the ESBL production should be routinely monitored especially among the K. pneumoniae strains for the effective management and control of such MDR pathogens.

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Pakistan Journal of Zoology


Vol. 54, Iss. 4, Pages 1501-2001


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