Identification of Meat Species by PCR-RFLP Method using Single Set of Degenerative Primers
Usman Asghar1*, Muhammad Faheem Malik1, Umer Rashid2, Naeem Mahmood Ashraf2, Sumera Afsheen1 and Muhammad Hashim1
1Department of Zoology, University of Gujrat, Hafiz Hayat campus, Punjab, Pakistan; 2Department of Biochemistry and Biotechnology, University of Gujrat, Hafiz Hayat campus, Punjab, Pakistan.
*Correspondence | Usman Asghar, Department of Zoology, University of Gujrat, Hafiz Hayat campus, Punjab, Pakistan; Email: email@example.com
2% Agarose gel visualized in transluminator, all lanes from left to right containing the DNA, which was extracted from goat, dog, cow, buffalo and donkey by using phenol-chlorophorm method.
PCR amplification result of goat, dog, cow, buffalo and donkey, in all species cytochrome b gene fragments have 400 bp length, M represent the 1kb ladder (Thermo Fisher).
After the RFLP of 400bp amplified product with TfiI restriction enzyme, gel electrophoresis along with 100bp DNA ladder, give different length fragments of goat, dog, cow, buffalo and donkey, these RFLP profiles are species specific and helped to easily and unambiguously differentiate between them.