Isolation and Identification of Mycoplasma mycoides Subspecies mycoides from the Ear Canal of Cattle in Plateau State, North-Central Nigeria
Isolation and Identification of Mycoplasma mycoides Subspecies mycoides from the Ear Canal of Cattle in Plateau State, North-Central Nigeria
Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa
ABSTRACT
This study was undertaken to isolate and identify Mycoplasma mycoides subspecies mycoides (Mmm) from the ear canal of apparently healthy cattle in Plateau State, Nigeria. One hundred and sixty six ear swab samples (n=166) were cultured from which eight (8) Mycoplasma species were isolated and characterized using conventional biochemical tests and polymerase chain reaction (PCR), respectively. Six (6) were observed to ferment glucose, reduce tetrazolium chloride and hydrolysed casein. They had no phosphatase activity, did not produce ‘film and spots’ and neither hydrolysed arginine nor urea and were preliminarily identified as members of the Mycoplasma mycoides sub-cluster which comprises Mmm and Mycoplasma mycoides subspecies capri (Mmc). Three (3) of the 8 Mycoplasma isolates were identified as Mmm using the IS1296F/R(all) and MM450/MM451 primer sets. The 574 bp product obtained by MM450/MM451 primer set gave two fragments (379, 178 bp) on digestion with AsnI restriction enzyme further confirming the isolates. This is the first report of the isolation and molecular identification of Mmm from the ear canal of cattle in Nigeria. It is recommended that samples from the ear canal be considered when Mmm isolation is intended so as to improve the chance of recovery.
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