This study was carried out to evaluate the diagnostic utility of microRNA-122 (miR-122) for liver injury detection involved in feline infectious peritonitis (FIP). Twelve cats were enrolled in this study. Cats were admitted to small animal hospital, Faculty of veterinary medicine, Cairo University. Cats were classified into six apparently healthy cats as a control group and six clinically diseased cats. All cats were exposed to clinical examination, abdominal ultrasonography, serum biochemical analysis as well as rapid feline infectious peritonitis (FIP) test and Rivalta test for the diseased cats. On basis of these results, six cats diagnosed with feline infectious peritonitis (effusive form) involving liver injury. Serum microRNA-122 was estimated by real-time polymerase chain reaction in all cats. Effusive form of FIP with liver injury was manifested by abdominal distension with fluid, anorexia, icteric buccal mucous membrane, lethargy. Biochemical analysis showed significant elevation in alanine aminotransferase (ALT), aspartate aminotransferase (AST), and Globulin with significant reduction in albumin and A/G ratio. Abdominal ultrasonography revealed anechoic ascetic fluid in between internal organs, abdominal lymphadenopathy, and peritoneal adhesion. Serum microRNA-122 analysis showed significant elevation by 11.31 fold change compared to control cats. In conclusion, microRNA-122 is of diagnostic value and stable biomarkers for liver injury detection in cats.
Keywords | Feline infectious peritonitis, MicroRNR-122, ALT, Biochemistry, Ultrasonography