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Molecular Detection of Methicillin-resistant Staphylococcus aureus (MRSA) from a Clinical Case of Myiasis Wound

Molecular Detection of Methicillin-resistant Staphylococcus aureus (MRSA) from a Clinical Case of Myiasis Wound

Pravin Mishra1, Md. Muket Mahmud2, Md. Ahosanul Haque Shahid2, Alamgir Hasan2, Vivek Kumar Yadav3 and Moinul Hasan1* 

1Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh; 2Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh; 3Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

moinul.vet@bau.edu.bd  

Figure 1

Collection of swab sample from myiasis wound. 

Figure 2

Fermentation of MSA by Staphylococcus aureus indicated by Smooth and convex formation of a yellowish colony. 

Figure 3

Staining characteristics of bacteria: Grape like clusters were found which are the characteristics of Staphylococcus aureus. 

Figure 4

Results of PCR of Staphylococcus aureus specific nuc gene (size=279 bp). Here, Lane 1: 100 bp ladder, Lane 2: negative control, Lane 3: positive control, Lane 4- amplified nuc gene of S. aureus. 

Figure 5

Amplification of the mecA gene (size=533 bp). Here, Lane 1: 100 bp ladder, Lane 2: positive control, Lane 3: amplified mecA gene of Staphylococcus aureus. 

Veterinary Sciences: Research and Reviews

June

Vol. 9, Iss. 1, Pages 1-86

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