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Molecular epidemiology and serological study of FMD virus in Egypt 2014

Molecular epidemiology and serological study of FMD virus in Egypt 2014

Hala K. Abdelmegeed 1, Eman M. Abohatab 1, Khattab,O. M. 1 , Salem, S.A.H. 1 , Arafa, A. 2, Nashwa M. Helmy 3

1 Virology Research Dept., 2 NALQP, 3 Biotechnology Dept.
Animal Health Research Institute, Dokki, Giza, Egypt

ABSTRACT

Foot and mouth disease (FMD) is an enzootic disease among the livestock in Egypt, affecting practically all cloven-footed domesticated mammals.In this study 161 out of 376 serum samples in percentage 42.8% were positive for FMD non structure proteins( NSP) antibodies which indicated the natural infection. ELISA kit used for FMDV antigen detection for the epithelial suspension from 42 field samples collected from various geographic locations 10 governorates are ( El-Sharkia,Giza, Port Said, Assuit, Suize, Kafre El-Shake, Qina, Al-Gahrbia, Domiatte and Alexandria) Serotyping and molecular characterization by polymerase chain reaction PCR for (31) samples as FMD Serotype O , (2) Serotype A and (3) serotype SAT2 the samples collected from outbreaks in period November 2013 –July 2014 the season Autumn and winter for forming an Epidemiological map for 10 governorates of the circulating strains during this period

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Journal of Virological Sciences

1

Vol. 9, Iss. 1, Pages 1-19

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