Mutational Analysis of PIK3CA Gene from FFPE of Non-Small Cell Lung Cancer in Non-Smokers
Mutational Analysis of PIK3CA Gene from FFPE of Non-Small Cell Lung Cancer in Non-Smokers
Farah Bilal*, Abdulmohsen Alhejaily, Shahida Husnain
ABSTRACT
PIK3CA is considered important component of PI3K pathway. Being a regulatory gene for RAS, PI3K and EGFR pathways, any change in PIK3CA gene sequence plays vital role in several cancers including lung cancer. Purpose of this study was to evaluate the prevalence of common nucleotide changes (E545K/E542K and H1047R/H1047L) occur in PIK3CA gene in non smoker patients of non-small cell lung cancer (NSCLC). Somatic mutational analysis was done by QMC-PCR following direct sequencing with sangers’ method. Only one sample detected p.E545K mutation at c.1633G>A. Another most common mutation was observed in PIK3CA exon 20 which leads to change A>G at codon 1047. This transition converts amino acid histidine to arginine. Our study concluded p.H1047R most frequent while p.E545K rarely found mutations in PIK3CA gene in NSCLC population. Present study can be proved as road map in setting PIK3CA mutations as potential therapeutics target for NSCLC in non-smokers.
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June 2019
Vol. 34, Iss. 1, pp. 1-104
