Phylogenetic relationship of Pakistani entomopathogenic nematode strains on the basis of ITS-1, 5.8S and ITS-2, D2-D3/28S (LSU) rDNA region and 12S rDNA mitochondrial gene
Phylogenetic relationship of Pakistani entomopathogenic nematode strains on the basis of ITS-1, 5.8S and ITS-2, D2-D3/28S (LSU) rDNA region and 12S rDNA mitochondrial gene
F. Shahina† and G. Mehreen
ABSTRACT
Entomopathogenic nematodes (EPNs) belong to families Steinernematidae and Heterorhabditidae have proved as
extremely useful biological control agents and infected a wide range of insect pests. Phylogenetic relationships
among Pakistani entomopathogenic nematode strains; steinernematids and heterorhabditid were estimated by
nucleotide sequences using three molecular makers viz; ITS-1, 5.8S and ITS-2 rDNA (seventy nine); D2-D3 and
28S (LSU) sequences of rDNA region (fifteen) and 12S rDNA mitochondrial gene (twenty nine) to investigate the
genetic diversity. Phylogenetic trees were constructed using two different methods maximum parsimony (MP) and
Bayesian inference (BI) in which most of them form highly to moderately supported clades.
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December 2014
Pakistan Journal of Nematology, Vol. 32, Iss. 2, Pages 123-240
