Screening of Puberty Related Differentially Expressed Genes from Ovary Tissues of Jining Grey Goat Based on Suppression Subtractive Hybridization
Screening of Puberty Related Differentially Expressed Genes from Ovary Tissues of Jining Grey Goat Based on Suppression Subtractive Hybridization
Yufang Liu1,2, Guiling Cao3, Yujing Xie3 and Mingxing Chu1*
ABSTRACT
To explore the molecular mechanism of the puberty effects on the reproduction of goats, goats at different growth stages from two breeds (Jining Grey (JG) goats and Liaoning Cashmere (LC) goats) were divided into three groups with three replicates (juvenile stage (JG 30d vs. LC 30d, AO group), puberty (JG 90d vs. LC 180d, BO group) and the same age control of puberty (JG 90d vs. LC 90d, EO group). Ovary tissues were taken from goats on 30 days, 90 days and 180 days, total RNA was extracted, differentially expressed (DE) gene libraries were constructed by suppression subtractive hybridization (SSH) technology, and puberty-related differential genes were screened. A total of 184 differentially expressed genes were screened, including 57, 68 and 59 in the AO, BO and EO groups, respectively. There are 22 differentially expressed genes were directly associated to the ovary development. According to the gene function analysis in KOG database, the known genes were clustered into 10 subdivisions in A group, 8 in E group and 9 in B group under three categories, mainly in posttranslational modification, protein turnover, chaperones, energy production and conversion, and transcription process. Pathway analysis in KEGG pathway database of the known genes revealed that the five pathways that most differentially expressed genes involving: GnRH signaling pathway, oxytocin signaling pathway, melanogenesis, thyroid hormone synthesis, and insulin secretion. Online tool was used to predict the function of DEGs, the results showed that the CYP11A1 gene might regulate the GDF9 and other prolificacy-associated markers in ovary organs of goats, thereby affecting the development of puberty. The expression of various genes and the transduction of hormone secretion signals, improving the reproduction of goats.
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