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Sensitivity and Specificity of the commonly used Diagnostic Procedures of Bovine Brucellosis

Sensitivity and Specificity of the commonly used Diagnostic Procedures of Bovine Brucellosis

H.I. Hosein1, Sherin Reda Rouby1*, Ahmed Menshawy1 and Ahmed E. AbdAl-Ghany2 

1Department of Veterinary Medicine, College of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt; 2Department of Hygiene, Zoonoses and Epidemiology, Minia University, Minia, Egypt.

sh_alrouby@yahoo.com,shereen.rouby@vet.bsu.edu.eg 

ABSTRACT

The present study was carried out for investigation of the sensitivity and specificity of some diagnostic procedures used for diagnosis of bovine brucellosis on serological, bacteriological and molecular basis. A total of 141 cows from brucella infected farms under quarantine of the veterinary authorities were employed. Serological examination using BPAT, RBT and CFT in 141 cows revealed 109 (77. 3), 105 (74.47) and 104 (73.76) seropositive respectively. Relative sensitivity, relative specificity, positive predictive value and negative predictive value of BPAT, RBT and CFT were estimated as, (98.04%, 76.92%, 91.74% and 93.75%); (94.33%, 85.71%, 95.24% and 83.33%) and (93.46%, 88.23%, 96.15% and 88. 24%) respectively. Different tissues specimens of 104 confirmed seropositive cows under investigation including, retropharyngeal, prescapular, prefemoral, internal iliac, supramammary lymph nodes, udder and spleen as well as milk of 46 lactating cows were subjected for bacteriological studies for isolation and identification of Brucella organisms. Brucella melitensis biovar 3 could be recovered from 64 (61.5%) tissue specimens and 28 (60.9%) milk samples. Brucella cultures were further identified on molecular basis using universal and Bruce ladder PCR. 

 

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Veterinary Sciences: Research and Reviews

June

Vol. 8, Iss. 1, Pages 1-70

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