Utilization of Bombyx ceiba Seed Pods: A Novel Substrate for Cellulase Production through Solid State Fermentation using Response Surface Methodology
Utilization of Bombyx ceiba Seed Pods: A Novel Substrate for Cellulase Production through Solid State Fermentation using Response Surface Methodology
Sobia Nazir1, Muhammad Irfan1*, Muhammad Nadeem2, Hafiz Abdullah Shakir3*, Muhammad Khan3, Shaukat Ali4, Quratulain Syed2
ABSTRACT
In this work, Trichoderma viride was used for cellulase production in solid state fermentation (SSF) using seed pods of Bombyx ceiba as substrate. Three parameters were used such as inoculum size (1, 5.5 and 10%), medium volume (1, 3 and 5ml) and incubation period (3, 4, 5 days) to observed the maximum activity of cellulase using Box-Bhenken design of response surface methodology. Maximum activity of CMCase (0.700 IU/gds/min) and FPase (0.260 IU/gds/min) was observed on 4th day with inoculum size of 1% and medium volume of 1ml (for 5g substrate). The proposed model was highly significant as depicted by ANOVA. The results recommended that seed pods of Bombyx ceiba play important role in cellulase production, which is useful for the food industry, textile industry and ethanol production.
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December 2019
Vol. 34, Iss. 2, pp. 105-238
