Zafar Iqbal1* and Muhammad Khurshid2
...) and African cassava mosaic virus-coat protein (ACMV-CP) were used. However, immunocapture of ToLCNDV could only be achieved by using the TYLCV-CP antisera followed by PCR detection by using specific and degenerate primers. ToLCNDV is geographically wide spread Begomovirus (Family Geminiviridae), considered as a close relative of TYLCV, and cause severe losses for many economical important crops in tropical and subtropical regions of the world. This is the fi...
M. B. Meah
Biopesticides for crop growth and crop protection
...ot knot and leaf curl/mosaic of tomato and carrot in the field. Aqueous solution of garlic and allamanda tablet sprayed at 1:1, 1:2, 1:3, 1:3, 1:4, 1:5 conc. increased seed germination by 45-60%, completely eliminated damping-off, seedling blight and tip over and increased seedling vigour by 22-50%. The tablets retained the similar range of action over aging up to 90 days. Both garlic and allamanda tablets displayed similar inhibitory action against damping-of...
Sunita J. Magar and D.D. Nirmal
Effect of plant spacings and cultivars on spread of Yellow Vein Mosaic Virus (YVMV) disease
...Spread of yellow vein mosaic virus (YVMV) disease in different plant spacing 30 x 30 cm, 50 x 30 cm, 60 x 30 cm, 60 x 60 cm and 60 x 40 cm and using two cultivars, i.e., cv. Parbhani Kranti and Local indicated that the first appearance of symptom was earlier in cv. Local and late in cv. Parbhani Kranti. The rate of infection was higher at 45 to 60 days after sowing in both kharif and summer while in cv. Local it was higher at 30 to 45 days after sowing. Less i...
Shyam Singh and L.P. Awasthi
Plant products for the management of yellow mosaic disease of mungbean and urdbean
...xtracts on the yellow mosaic of mug bean. Maximum reduction in disease incidence recorded were 66.70 and 63.65 percent in mungbean and Urdbean, respectively by eight sprays of Clerodendrum aculeatum. Whereas, eight sprays of Boerhaavia diffusa root extract could reduce the disease incidence by 60.27 and 58.20 per cent followed by Azadirachta indica leaf extract by 42.43 and 42.92 percent in mungbean and urdbean, respectively. Maximum plant height (70.90 and 56...
Muhammad Mansoor1, Amanullah1, Zafar Islam2*,Sher Muhammad3, Muhammad Umair4, Mehmood Ayaz5, Asghar Ali Khan6, Muhammad Asif2 and Ya Sakina3
... leaf spot and Yellow mosaic virus.
...
Muhammad Hanif Munawwar*, Asghar Ali* and Shahid Riaz Malik*
IDENTIFICATION OF RESISTANCE IN MUNGBEAN AND MASHBEAN GERMPLASM AGAINST MUNGBEAN YELLOW MOSAIC VIRUS
...ainst mungbean yellow mosaic (begomo) virus (MYMV), 64 lines of mungbean and 21 of mashbean were evaluated under field conditions. Out of 64 mungbean lines, 6 were found as resistant (R) and 35 moderately resistant (MR). Sixteen accessions were graded as susceptible (S) whereas 7 were found highly susceptible (HS). In mashbean, 12 out of 21 accessions were resistant (R) and 8 moderately resistant (MR), whereas, only one was susceptible. Although resistance aga...
S. Talat Gilani*, Shahid Hameed* and Hussain Shah*
OCCURANCE AND DISTRIBUTION OF POTY VIRUSES INFECTING GARLIC IN PAKISTAN
.... The yellow stripes, mosaic and chlorotic spot symptoms of the disease resemble the viral infection in garlic reported to occur worldwide. Altogether 690 samples were collected from 29 locations of Punjab and 40 locations of Khyber Pukhtunkhwa to determine the prevalence of Onion Yellow Dwarf Virus (OYDV) and Leek Yellow Stripe Virus (LYSV). Serological testing DAS-ELISA technique was used to test the samples collected from the farmer fields. Based on the DAS...
Hongqun Li1, Xiaoli Liu2,*, Zhenmin Lian3,RenheWang4, Yongbin Wang4, Yongyao Fu1 andDingyi Wang5
Ghulam Abbas1, Khalid Pervez Akhtar1, Muhammad Ahsan2, Muhammad Jawad Asghar1, Fiaz Ahmad1 and Muhammad Rizwan3*
...ign: justify;">Yellow mosaic disease (YMD) caused by mungbean yellow mosaic India virus (MYMIV) is an important constraint of mungbean during the summer season in Pakistan. The use of resistant varieties is the only way to reduce the losses caused by YMD. Resistant sources had already been reported in mungbean, but no such information seems to be available for mung × mash interspecific recombinants. In this study, 72 m...
Gokhan Sahin1, Ecevit Eyduran2,*, Mete Turkoglu3 and Fatma Sahin2
...LOSS (Combined Photo Voltaic system losses) were calculated. Estimation of global irradiation parameters was made through multivariate adaptive regression splines (MARS) data mining algorithm for multiple responses (Hd and Hm) with the support of R software program and the utility prediction equation was aimed to improve for further biodiversity investigations. To determine the predictive quality of the MARS algorithm, goodness of fit criteria viz. coef...
Hao Gu*, Zhi Yang, Weiqiang Yu, Kan Xu and Yuan-fei Fu*
...ococcus sanguis. Capsaicin extracted from chili peppers were selected to explore the antibacterial activity against the four cariogenic bacteria in terms of acid producing and biofilm. The antibacterial activity of capsaicin was evaluated by the minimal bactericidal concentration, change of pH in the first 12 h and OD values of biofilms. Besides, fluorescent images of exopolysaccharides and living bacteria of Streptoc...
Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1
...ainst Mungbean yellow mosaic virus (MYMV) disease. Exogenous use of Salicylic acid (SA) and Benzothiadiazole (BTH) as elicitors enhanced the resistance in mungbean plants by triggering the SA pathway. Induced resistance was assessed by evaluating the appearance of symptoms and detection of virus titter through ELISA. Different concentrations of SA and BTH were exogenously applied to activate the inherent resistance of mungbean by the production of defense asso...
Farhat Iqbal1, Abdul Waheed2,*, Zil-e-Huma3 and Asim Faraz2
Muhammad Waqas, Shahid Ali*, Syed Attaullah Shah and Ghaffar Ali
A. S. M. El-Nuby1† , S. A. Montasser2 and I. A. El-Khadrawy
Control of root-knot nematodes using wild plants colonized Sinai, Egypt
...lants viz., Artemisia judaica, A. monosperma, Bassia muricata, Cornulaca monacantha, Salsola kali and Zygophyllum album with different dilutions (25%, 50%, 75% &100%). The potent nematicidal efficacy observed in the extract of A. judaica followed by A. monosperma. In vivo trial post inoculation treatments were effective than pre one, the maximum reduction in nematode population was recorded by A. jud
Khizar Azam1*, Abdul Shakoor1, Shahid Maqsood2, Muddasar Habib3, Muhammad Fahad4, Akhtar Zaib1
IDENTIFICATION OF SITES FOR ESTABLISHING PHOTOVOLTAIC SOLAR FARMS IN PAKISTAN USING SOLAR GEOMETRY
...tiple arrays of Photovoltaic (PV) Panels which collect and convert
solar energy into electrical energy. Pakistan is facing a serious energy crisis. It is working on solar energy options.
Therefore, it is imperative to identify most viable sites that have a high solar radiation. We utilized solar geometry
and the Google Earth, a geographical information program to identify optimal sites for Pakistan. These sites should
also include b...
solar energy into electrical energy. Pakistan is facing a serious energy crisis. It is working on solar energy options.
Therefore, it is imperative to identify most viable sites that have a high solar radiation. We utilized solar geometry
and the Google Earth, a geographical information program to identify optimal sites for Pakistan. These sites should
also include b...
Zahoor Ahmed Baloch*
IMPLEMENTATION OF TAST CODES IN DS-CDMA COMMUNICATION SYSTEM
...nciple of threaded algebraic space-time (TAST) block code and DS-CDMA are retrospectedand
their integrationare studied and analyzed. Simulation results show that a substantial improvement in bit error rate
(BER)can be achieved in CDMA networks by integratingTAST codes in it. ...
their integrationare studied and analyzed. Simulation results show that a substantial improvement in bit error rate
(BER)can be achieved in CDMA networks by integratingTAST codes in it. ...
Samet Hasan Abaci1,*, Hasan Onder1, Mustafa Sahin2 and Esra Yavuz3
Kadir Karakuş1, Turgut Aygün2, Şenol Çelik3, Mohammad Masood Tariq4*, Muhammad Ali4, Majed Rafeeq4 and Farhat Abbas Bukhari4
Hamed A. Ghramh,1, 2, 3,Zubair Ahmad 1, 2, 4, Khalid Ali Khan1, 2, 3*and Farhat Khan4
.... nov., M. faifaicus sp. nov. and M. khamisicussp. nov. (Hymenoptera: Braconidae: Microgastrinae) are described. The genus Microplitis is recorded for the first time from Saudi Arabia. Characters of these new species and their affinities with related species are discussed.
...
Iram Bilqees1, Yasir Iftikhar1, Mustansar Mubeen1,2*, Qaiser Shakeel3, Ashara Sajid1, Zahoor Hussain4, Aqleem Abbas2, Muhammad Aamir Sohail2, Judith Jeruto Kiptoo5 and Shehzad Iqbal2
Use of Nutrients and Plant Extract to Manage Okra Yellow Vein Mosaic Disease (OYVMD) in Sargodha, Punjab, Pakistan
...fy;">Okra yellow vein mosaic disease (OYVMD) has been a menace to okra cultivation wherever grown in the world. This trial was carried out to monitor the disease severity, incidence and its management through different nutrients and plant extract. Plant extract and multi-nutrients were used to manage the disease to avoid the hazardous effect of chemicals on human health and being the eco-friendly. Four varieties of okra viz., Indian Rachna, Sabz Pari, Desi Bhi...
Muhammad R. Khan1, Bushra A. Rakha1,* and Muhammad S. Ansari2
...hizothorax and Schizothoraicthys were investigated. The species found in river Swat were Schizothorax plagiostomus, Schizothoraicthys esocinus, Schizothoraicthys labiatus, Schizothorax richardsonii, Schizothorax sinuautus and Schizothoraicthys macropthalmus. Among these species, Schizothorax richardsonii is considered as vulnerable a...
Ghulam Rabbani1, Uzma Javed1*, Javed Iqbal2, Ruqeah Mustafa3, Ghulam Shabbir2 and Fida Hassan Shah4
Barani Mash a Newly Developed Disease Resistant and High Yielding Mash Cultivar for Rainfed Areas of Punjab, Pakistan
... resistance to yellow mosaic. The main yield contributing characters were number of pods per plant, number of primary branches per plant, yield per plant and 100-kernal weight. Based on desirable traits and higher yield, this line was approved with name of “Barani Mash” by the Punjab Seed Council for its cultivation on commercial level in rainfed areas of Punjab. “Barani Mash” will prove to be a good alternative of existing cultivars an...
Muhammad Tahir Waseem1, Abdul Majid Khan1*, Abdul Ghaffar2, Ayesha Iqbal1 and Rana Manzoor Ahmad1,3
...d to have inhabited a mosaic of woodland and grassland with predominant C3 vegetation which latter shifted to C3/C4 grasses. For this purpose, fifteen dental samples (Five for each Hipparion species) from well dated late Miocene localities between 8.8-7.7 Ma are analyzed. All the δ13C values (V-PDB) are in range of -11.27‰ to -3.36‰ which represent that these horses dominantly inhabited C
Muhammad Ramzan1*, Unsar Naeem-Ullah2, Muhammad Umair Sial2, Naeem Iqbal2 and Shafqat Saeed2
..., F. benghalensis, F. caraica, F. infectoria, F. elastica, F. nitida, Artocarpus communis, A. heterpphyllus and A. kamansi. Fairly limited studies are documented for its chemical and biological management. For chemical control, deltamethrin was proved more toxic while among biological control agents Goryphus sp., Enicospilus sp., Telenomus sp, Listrognathus spinifrons, Trichogramma pretiosum, T. euproctidis and T. dendrolimi have been documented as potential e...
Mandour, A. M. 1, Abdel Maksoud, H. M. 2, Amal, A. Khalil 3 and Mohga, A. El-Tahlawey 4
...o rested. Maize dwarf mosaic virus (MDMV) is the most
economically significant virus infecting maize crop in Egypt. The percentage
of infection differs from one location to another. MDMV was isolated from
naturally infected maize plants at El-Monfia. These samples were checked
for virus presence using Indirect ELISA. A number of cultural practices were
evaluated as control measures against MDMV to min...
Allam A. Megahed1; Hoda M.A. Waziri2; Khaled A. El-Dougdoug3; Badawi A. Othman3; Sirag M. Lashin1; Mohamed D. Hassanin1 and Mahmoud A. Ibrahim4
...trum of purified Beet mosaic virus (BtMV) particles obtained by density gradient centrifugation revealed that, the concentration of virus preparation estimated using spectrophotometric measurements at 260 nm was 3.004 mg/ml. The min, max nm, Amax./Amin., A260/A280 and A280/A260 ratios were 244, 260, 1.276, 1.512 and 0.662, respectively for BtMV. The corresponding figures of the purified virus preparation of Beet necrotic yellow vein virus (BNYVV) were 2.196 mg...
M. A. S. El-kady1; A. B. Badr2; Ahmed K. El-Attar1, Hoda M. A. Waziri1 and Kh. E. A. Saker1
A. B. Badr1 ; M. A. S. El-kady2; and Kh. E. A. Saker2
.../div>...
Amel, S.M. Abo-Senna1; M.A. Nasr-Eldin2; B.A. Othman3 and A.A. Megahed4
.../p>...
M. A. Kararah1, Om-Hashem M. El-Banna1, Salwa N. Zein2 and Abd-Elrehiem, A.F.3
...the present work, Pea mosaic (PMV) was first reported on cowpea
(Vignaunguiculata (L.) Walp).PMV was isolated from naturally infected
cowpea plants, showing mosaic and choloratic ring spot symptoms, which
had been grown in the experimental fields of Giza Agricultural Research
Experimental Station (A.R.E.S) in 2008. Identification studies based on host
range, symptom...
Megahed, A.A.1; Kh. A.El-Dougdoug2 and B.A. Othman2
...incidence of Cucumber mosaic cucumovirus (CMV), Beet mosaic potyvirus (BtMV), Beet necrotic yellow vein benyvirus (BNYVV), Beet curly top geminivirus (BCTV) and Beet yellows closterovirus (BYV) symptomatology and serologically by double antibody sandwaich Enzyme linked immunosorbent assay (DAS-ELISA). The survey of sugar beet viruses achieved in 15 field locations belonging to 5 different ...
Manal A. El-Shazly1. M. I .Kobeasy2and Sarah H . Altalhi3
...symptoms circumvented mosaic, curling, bronzing and/or purpling, chlorosis and necrotic spot on the leaves. Disease symptoms of infected fruits produced from inoculated healthy tomato seedling showing discoloration, faint concentric rings, necrotic and chlorotic spot on mature tomato fruits. The virus was biologically purified from a single local lesion formed on Chenopodium amaranticolor Caste & Reyn. The isolated virus was identified on the basis of symp...
Radwa M. Shafie 1 and Soha S. M. Mostafa2
..., against Bean yellow mosaic virus (BYMV) infection on Vicia faba L. Giza 716 and Chenopodium amaranticolor Cost and Reyn. The non-inoculated algal synthetic media were used as control. Findings revealed that the mixture of the three algal filtrates gave the highest percentages of inhibition. Lower inhibition effect was produced by culture filtrate of N. muscorum and S. platensis, respectively in particular in plants treated with algal filtrates by mixed with ...
Salama M. El-Saghir1, 2
...ptoms, viz. rugosity, mosaic, stunting, leaf bronzing, and early
leaf dropping. Heavy losses; however, can occur upon mixed infection with PVS and PVX or PVM.
Objective: The objective of this study is to characterize the incidence of PVS serologically with
authentic antiserum for PVS and, molecularly using specific primers for PVS.
Methods: In-direct ELISA (I-ELISA) and dot blotting immunobinding assay (DBIA) wer...
Maha A. El-Abhar1, Moustafa A. Elkady1, Khaled M. Ghanem2, Hussieny A. Bosila3
...v>Background: Alfalfa mosaic virus (AMV) is only virus in the genus Alfamovirus and has very wide host
range among weed and crop plants which produces a variety of symptoms. It can cause problems in potato
in some regions where vectors easily move into potato fields from reservoir host, particularly if a tuber
necrosis-causing strain is involved.
Objective: : The purpose of this study is to characterize biologica...
Mohsen M. Elsharkawy1, Salem H. Homayed1, Mohamed M. Elsawy2 and Amr A. Khedr1
...>Background: Cucumber mosaic caused by Cucumber mosaic virus (CMV) is a serious threat to crop
production all over the world.
Objective: The aim of the current investigation was to evaluate the potential of fan-forced wind and
the growth promoting fungus, Fusarium equiseti GF 19-1, as novel and safe management strategies
against the invasion of CMV in cucumber plants.
Ahmed K. El-Attar1, Samah A. Mokbel1 and Om-Hashem M. EL-Banna2
...s isolates of Alfalfa Mosaic Virus (AMV) found throughout the world
affect a wide variety of aromatic and medical plants. In March 2016, several symptoms of leaf
necrosis, bright yellow mosaic and malformation of leaves suggested viral infection of AMV on
basil plants grown in Beni Suef governorate.
Objectives: The present study aimed to characterize the virus at the molecular ...
Ahmed M. Soliman
...>Background: Cucumber mosaic virus (CMV) is one of the widest spread viruses in the world and has the largest host range of plants.
Aim: The present study was conducted to identify four Saudi CMV isolates (cucumber, tomato, pepper and watermelon) using biological, serological and molecular assays.
Methods: Survey of some vegetable crops exhibiting mosaic, yellowing, blisters, shoestring leaf, mottling...
Mohsen M. Elsharkawy1, Sara E. Hanbal2, Samir A. Sidaros1, Mohamed M. Elsawy2, Ali H. Hamed2
...ckground: Bean yellow mosaic virus (BYMV) is recognized as one of the most wide distributed virus disease affecting faba bean in Egypt.
Objective: The aim of this study is to explore the efficiency of the plant growth promoting fungi (PGPF) of Penicillium simplicissmum GP17-2, Fusarium equiseti GF19-1 and Trichoderma asperellum SKT1 as a sustainable method to control the invasion of BYMV in faba bean plants.
Methods: Faba bean plants we...
Samah A. Mokbel, Eman A. Ahmed, Hanan F. El-Kammar, Ahmed A. Kheder
...>Background: Cucumber mosaic virus (CMV) is known to occur in sugar beet plants in Egypt and may
produce severe damage to infected plants. However, studies on the effect of the CMV on the cellular
and internal structures of sugar beet leaves were rare.
Methods: The CMV was isolated from sugar beet samples collected during November 2018 from the
Fayoum governorate, exhibited symptoms of mos
Amin Arif1, Khizar Samiullah1*, Riffat Yasin2, Bilal Rasool1, Shakila Naz1, Xijun Ni3 and Saleem Akhtar1
...ls the persistence of mosaics of diverse habitats ranging from tropical evergreen forest to subtropical ones, closed seasonal woodlands to wooded savannas during the deposition of the Dhok Bun Ameer Khatoon, Chinji Formation.
...
Zahid Iqbal*, Farhat Ullah Khan and Jalal-ud-Din
Honnakerappa S. Ballari1 and Shashikant S. Udikeri2*
...molecules over decades. Raichur populations comprehend high level of resistance to Pyrethroids with LC 50 121.63 ppm to cypermenthrin which indicated 23.21fold of resistance with respect to a laboratory susceptible strain. Similarly, there was 24.95 folds of resistance to deltamethrin in same population with LC 50 128.47 ppm. Among organophosphates a high level of resistance was recorded against monochrotophos with LC50 160.94 ppm and 18.23 resistance ratio in...
Nausheen Irshad1, Maria Akhter1, Tariq Mahmood2*, Faraz Akrim3, Muhammad Rafique Khan1 and Muhammad Sajid Nadeem4
...Altai weasel (Mustela altaica), a “Near Threatened” mammal, in Bunjosa Game Reserve, Azad Jammu and Kashmir, Pakistan. The species occurrence was confirmed by direct field sightings (live and kill), and indirect signs (pugmarks, burrows, fecal droppings, and reported sightings by local community). During one-year study period (September 2015-August 2016), a total of 28 individuals of the species were sighted in the field including 18 live and 10 de...
Asmaa A. Badr, Eman A. Abo Elfadl, Mohammed M. Fouda, Sayed M. Elsayed
Samreen Fatima* and Muddasir-Uddin
Pharaoh C. Sianangama1*, Brian Tembo2, Sylvia J. Harrison1, Rubaijaniza Abigaba1,3
Shad Mahfuz1,2†, Hong-Seok Mun1,3†, Veasna Chem1, Keiven Mark B Ampode1,4, Muhammad Ammar Dilawar1,5, Hae-Rang Park1, Il-Byung Chung1, Young-Hwa Kim6, Chul-Ju Yang1,5*
...six (6) ingredients (capsaicin, red chili, ground garlic, dead insects, denatonium benzoate, and thiophanate-methyl) were used as repellents. The repellent ingredients were used to hang over the feeders. Among the tested natural and chemical ingredients as a repellent, the average number of feeding approaches in repellent feeder was significantly lower (P < 0.05) in bitrex chemical (denatonium benzoate) and thiophanate-methyl chemical. No differences were n...
Hanaa H.A. Gomaa1*, Dalia Y.Z. Amin1, Mona A. Ismail1 and Khalid A. El-Dougdoug2
...o yellowish mottling, mosaic spots and deformation were observed in leaves of fig plants. To evaluate the presence of Fig latent virus (FLV -1) in fig plants. One hundreds fig trees were collected with virus-like symptoms and symptomless fig trees. The virus was detected by DAS-ELISA. Infected fig leaves were mechanically inoculated on Chenopodium amaranticolor L. and reinoculated on Ch. amaranticolar L. and Nicotiana glutinosa for virus propagation. Fresh woo...
Adefunke Fadilat O. Ayinde1*, Peter Allison Johnston2, Olanrewaju Olusoji Olujimi3, Purnamita Dasgupta4 and Dare Akerele5
... to withstand cassava mosaic virus disease, extreme weather conditions, and its long gestation period. Government of both states should provide infrastructure support to improve the cassava-based farmers’ adaptive capabilities to climate variability and reduce their vulnerability....
Ansar Abbas1*, Muhammad Aman Ullah2, Abdul Waheed3 and Muhammad Asif4
Sumreen Begum, Atta-ur-Rahman and Asmat Salim*
Om-Hashem M. EI-Banna1, M. A. S El-Kady2, E. A Salama1 and Salwa N. Zein2
... 18) of Barley Stripe Mosaic virus (BSMV) revealed a high degree of sequence similarity of γ RNAs comparing to ß RNAs of both strains. On the Other hand, nucleotide, and amino acid sequences of the two strains revealed differences in the 5' untranslated region (UTR) between the coat protein and ꞵb gene. It was clear that five bases, and 18 out of 1 16 amino acids were found different. three amino acids insertion, and one amino acid deletion. Litt...
Characterization and Molecular Detection of Tomato spotted wilt Tospovirus Infecting Tomato in Egypt
Hayam S. Abdelkader1; Gamalat M. Allam1; T. A. Moustafa2; and M. El-Hanunady2
...ic necrosis, systemic mosaic yellowing, bronzing, or stunting. The virus transmitted by mechanical means as well as by Thrips tabaci. Its TIP was between 45-50oC, DEP was 10-5 and LIV was between 5-6 hrs. Local lesion technique for biological purification and electron microscopic analysis confirmed the production of mature infectious virus particles underlining the conclusion that a full infection cycle was completed in this system. Both the structural viral p...
Sahar. A. Shoman1 and B. A. Othman2
... detection or Tobacco mosaic virus- Egyptian strain (TMV-E). Total RNA extract of infected plant and RNA of purified TMV particles were subjected in this method with four degenerate and undegenerate primers. The primer pairs generated two specific PCR fragments of 3428 base pair (bp) and 3836 bp of the whole TMV genome. The intensity of these RT-PCR amplified products was estimated, and it indicated relatively to the amount of the virus in the infected plant, ...
M.A.S. El-Kady1, Om-Hashem M. El-Banna 2, E.A Salama2, Salwa N. Zein1
...late Of Barley stripe mosaic Hordevirus (BSMV). The 1st procedure was successful in producing relatively high yield of virus preparation 3.7 mg/100 g of barley leaves with adequate purity and one light-scattering zone was round in sucrose density-gradient column. The 2nd and 3rd procedures yielded aggregated virions (2.3 and 7.0 mg/ 100 g of fresh barley leaves, respectively). Electron microscopy of negatively stained purified virus preparation showed rod-shap...
A. S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik2; Nashwa M. A. Abd El Mohsen1; and H. M. Abdelmaksoud1
...ific to Potato aucuba mosaic virus (PAMV). Electron microscopy showed spindle-shaped like inclusions as aggregated virus particles slightly flexuous in longitudinal and cross sections of PAMV infected cells: the Vitus induced different abnormalities in the structure of chloroplasts and mitochondria. The CP genes of PAMV were detected using RT-PCR with specific primers. ...
A.l. Abd El-Fattah; A.s. Saclik M.M. El-Kholi; I.A. Åbdcl-Hmnid and M.A. Madkour
...eight:108%">Sugarcane mosaic pot.1Virus (SCMV) is the causal agent Of mosaic disease which is reported to be one of the most important viral diseases attacking sugarcane crop in Egypt. Therefore. the present work was designed to screen the SCMV strains in Egypt based on biological. serological and molecular characterization. Data of the use of Sorghum bicolor vars. Rio and Atlas show successful detection of 7 SCMV strains (A...
Naeem Akhtar1*, Sana Noureen1, Muhammad Asif2, Ahsan Aziz2, Usman Saleem1, Talat Mahmood3, Nadeem Raza4 and Waqas Raza5
...ic factors, sugarcane mosaic virus (SCMV) is one of the most devastating factors that causes significant losses of sugarcane production. Here we are reporting the assessment of genetic variation among sugarcane accessions based on morphological parameters, SCMV severity, incidence and resistance/tolerance. The experiment was conducted in College of Agriculture, University of Sargodha, Sargodha, Pakistan using randomized complete design in triplicate. Different...
Mervat M. Fath- Allah, Amal A. Ahmed, Hala A. Amin
...th Bean yellow
mosaic virus (BYMV), Carnation vein mottle virus(CVMV), Potato virus Y, Watermelon mosaic virus
(WMV) and Zucchini yellow mosaic virus (ZYMV) of potyviridae family. A potyvirus universal
degenerate primer was used to amplify the polyprotein region of members of Potyviridae. The RTPCR
revealed 335 bp amplified products with only infected plant samp...
mosaic virus (BYMV), Carnation vein mottle virus(CVMV), Potato virus Y, Watermelon mosaic virus
(WMV) and Zucchini yellow mosaic virus (ZYMV) of potyviridae family. A potyvirus universal
degenerate primer was used to amplify the polyprotein region of members of Potyviridae. The RTPCR
revealed 335 bp amplified products with only infected plant samp...
...lants featured severe mosaic on Lentil cv. Giza51, mottling on cv.Giza9, mild mottle on cv. Giza 4, yellow mild mottling on cv. Giza370. Seed transmission tests of PMoV confirmed of the virus transmission through Lentil seeds. Peanut Mottle Virus was detected in the testas, cotyledons and embryo dissected from seeds of each Lentil cultivates. Electron microscopy of PMoV showed virus particles aggregates and degenerated ...
Hafiz Nawaz1*, Kashaf Nawaz2, Attiq ur Rehman1, Muhammad Bashir3, Mussera Hira2 and Mariyam Nawaz4
...ally. Mungbean Yellow Mosaic Disease (MYMD) is a whitefly-transmitted viral disease. Infected plants have uneven yellow-green spots. Diseased plants develop late and produce few flowers and pods. MYMD causes 85% of economic losses. Begomovirus is a Begomovirus. DNA-A and DNA-B make up its single-stranded genome. The virus’s virulence and symptoms need both components. The sequencing of both components showed considerable variation between old-world and n...
Mohga A. El-Tahlawey1, Samah. A. Mokbel1, A.M. Mandour1,and H. A. Mohamed2
...plants feature severe mosaic on lentil cv. Giza51. mottling on cv. Giza9. mild mottle on cv. Giza4, yellow mild mottling on cv. Giza370, Seed transmission tests Of PMoV confirmed of the virus transmission through Lentil seeds. 
Peanut mottle virus was det...
Peanut mottle virus was det...
El-Bramawyl , M . A. S. A. and El-Beshehy2, E. K. F.'
...justify;">Bean yellow mosaic virus (BYMV) is one of a devastating pathogen that reduces faba bean crop production in Ismailia. Resistance is limited and resistance-breaking isolates are becoming problematic. Effective BYMV disease control strategies will come from a better understanding of inheritance the resistance to BYMV disease in the crop considering. In this article we consider the determination role of the inheritance mode of the resistance to BYMV in F...
Elbeshehy, E. K.F. and Sallaml, A.A.A.
...n: justify;">Cucumber mosaic virus (CMV) was isolated from naturally infected cucumber plants Cucumis sativus L. grown in various garden and greenhouses of Ismailia Governorate, Egypt exhibiting systemic mosaic, blistering, fruit malformation and stunted plant growth and identified by biological, serological and molecular analysis. The isolated virus gave positive reaction with CMV antiserum but not with antibodies of WW and...
El-Dougdoug2, Kh.A; Ghalyl, M.F. and Tahal , M.A.
...to control a Cucumber mosaic virus (CMV). In the 1st experiment, One half of leaves of Chenopodium amaranticolor were treated with culture filtrate (CF) followed by CMV inoculation on both halves. In the 2nd experiment, The first pair of Cucumis sativus leaves were teated CF with CMV mechanically inoculated onto one leaf, the other non-treated leaf was CMV inoculated after 7 days of treatment. In 1 st experiment, CF treatment was able to considerably reduced t...
Mahdyl , A.M.M.; Hafezl , M.A.; EL-Dougdoug2, Kh. A.; Fawzyl , R.N. and Shahwanl , Eman S.M.•
...lant against Cucumber mosaic cucumovirus (CMV), salicylic acid (SA) activities were determined in the inoculated and non-inoculated tomato plants. Identify of CMV was confirmed by some differential hosts and dot blot immunoassay (DBIA) using polyclonal antibody specific CMV. Water extracts of Mirabilis jalapa, Clerodendrum inerme and Kombucha as antiviral to control the virus infection and detection systemic acquired resistance (SAR) Were Studied. SAR was dete...
Nassarl , Entsar A.; El-Dougdoug , Kh. A.; Osmanl , M.E; Dawoud3, Rehab
A. and Kinawy l , Aliaa H.*
...santhemum plants with mosaic, mottling and flower discoloration. The virus was purified biologically using serial transfer of the single local lesion technique on Nicotiana gultinosa. The induced antiserum for the isolated virus had a titer 1\1024. 600 bp DNA fragments from the coat protein gene (CP) of TMV—Ch—EG was amplified with Rt-PCR technique. Phylogenetic analysis of the TMV-Ch-EG/CP- gene showed 89% nucleotide sequence homology with other p...
Nasr-Eldin1 , M. A.; El-Dougdoug2, K. A.; Othman2, B. Ahmedi , Sabah A, and Abdcl-Azizl , S. Il.
...cific symptoms severe mosaic, vein clearing, rugosity and yellowing spots. CEVd was isolated on Gynura aurantiaca plants which showed specific symptoms mild mosaic and mottling and Lycopersicon esculantum L. cv. Castle rock reacted with PSTVd producing leaf curl and epinasty.
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Khatab, Eman A.H.; Zein, Salwa N. and Ahmed, Amal A.
...ify;">Broad bean true mosaic virus was purified from infected faba bean plants. The UV absorption spectrum had a maximum at 260 nm and a minimum at 240m. The ratios of Amax /A min and A 260/A 280 were 1.48 and 1.71, respectively. The yield of purified BBTMV preparation was about 1.57 mg/Kg infected leaves. Production of specific antiserum against BBTMV was obtained by rabbit immunization using three different methods of injections. The titer Ofthe prepared ant...
Ahmed, AmalA.; Zein, Salwa N. and Khatab, Eman A. H.
...ign: justify;">Celery mosaic Potyviruses (CeMV) was detected and isolated from naturally infected celery plants showing mosaic symptoms suspected to be caused by viral infection, also, it was isolated from other hosts. The isolated virus was biologically purified from single local lesions formed on Chenopodium amaranticolor. CeMV was identified by its host range, symptom expression, modes of transmission and particle morphol...
Zein Salwa N; Abd El-khalik, Samaa; Khatab , Eman A.A.H and Azzam4,Clara R.
...rst record of Tobacco mosaic Tobamovirus (TMV-S) on sunflower in Egypt. It was originally isolated from naturally infected sunflower plants growing in Giza Res. Station, showing systemic mosaic and spots. Purified TMV-S migrated as a single zone in density gradient column. Ultraviolet absorbance ofTMV-S was typical of nucleoprotein with minimum and maximum at 247 and 260 nm respectively. The ratios of A260/280 and Amax/min w...
Alhudaib, Khalid
...affected by fig plant mosaic diseases (Fig leaf mottle-associated virus, Fig mosaic virus). The main symptoms are chlorotic mottling, blotching and various types of leaf deformation. Samples were collected, with consideration of the economically importance and distribution of the cultivars, from different areas of Hofuf Saudi Arabia. Each sample was consisted of 10-15 leaves. Samples were labelled and stored in plastic bags ...
*El-Helaly, Sahar H.; ** Ahmed, Amal A.; * Awad, M.A. and ** Soliman, A.M.'
...gn: justify;">Alfalfa mosaic virus (AMV) was isolated from naturally infected potato (Solanum tuberosum, L. cv. Spunta) plants, showing bright yellow blotching or mottling «Calico» and aucuba symptoms suspected to be due to virus infection. Leaf samples were collected from different localities in Minufyia Governorate, Egypt. AMV was readily mechanically inoculated by sap extracted from infected potato leaves to host plants (bioassay). The identity ...
Ahmed, Amal A. and Fath-Allah, Mervat M.
...n: justify;">Cucumber mosaic virus(CMV) and Plum pox virus(PPV) (Sharka) were isolated from naturally infected apricot (Prunus armeniaca cv. ElAmar) trees showing leaf mottling, marginal leaf necrosis deformation and abortive flowers grown at Kalubia and el-Fayoum Governorates, Egypt. The identities of the two viruses were confirmed serologically using DASELISA. Graft inoculation of diseased apricot scion onto healthy one showed the same symptoms as those seen...
S.A. Sidaros, S.A. El-Kewey , Hala A. Amin;Eman A.H. Khatab , A.A. Emeran l , Samaa Abd El-Khalilk and M.A.S. El-Kady
...om Tropica Soda Apple Mosaic Virus-CP (TSAMV-CP) Florida isolate (USA) of Tobomovirus...
Sahar, A. Youssef t ; M.M.A. Al-Dhaher 2 and A.A. Shalaby
...cluding yellowing and mosaic pattern or bright yellow bands along major veins. Fan-shaped leaves may or may not be present with mosaic or vein banding symptoms which identified as Grapevine fanleaf virus (GFLV). Detection of the viruses was carried out by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) and reverse ü-anscription-polymerase chain reaction (RT-PCR). The use of tissue culture was inve...
* Amal Abou El-Ela, A.
...arviruses i.e., Apple mosaic virus (ApMV), Prunus necrotic ring virus (PNRSV), Rose mosaic virus (RNIV) and Tobacco streak virus (TSV) Preliminary results revealed the presence of PNRSV in tested rose shrubs. The isolated virus was biologically purified from single local lesions formed on cucumber cotyledon. Identification of this virus was based on host range, properties in crude sap, transmissibility, and serological tests...
Manal A. El-Shazly1, A. s. 2 Abdel Wahab and Salwa N. Zein3
...oms included systemic mosaic and yellowing for TSWV and necrotic eyelike spots and yellow spots on leaves, and flower stem for IYSV. Both TSWV and IYSV were mechanically and seed transmitted. TSWV was transmitted by two different thrips species, Thrips tabaci L (33.3%) and Frankliniella occidentallis Pergamde (60.9%) whereas the transmission of IYSV was obtained by Thrips tabaci L. only (45%). Adults of T. tabaci and F. occidentallis Pergamde as vectors ...
S. A. Sidaros*, S. A. EL-Kewey*, Eman A. H. khattab**, M. M. ELsharkawy*
Mohga M. El-Tahlawey, A. Mandour and AE Aboulata
...tic virus (FBNYV) and mosaic causing viruses were inspected during Oct. 2003 to Apr. 2004 on Faba bean fields grown in Qalyubia governorate. Population density of seven different aphid species on faba bean was arranged descendingly as follow: Aphis craccivora (270.9). Myzus persicae. (89.0). A fabae (52.5). Acyrthosiphon pisum (24.3), A. gossypii (4.7). A sesbaniea (1.8). and A. nerii (0.7). Population fluctuation of previous aphid species was also recorded on...
A. A. Kheder1; I. A. M. Ibrahim2; H. M. Mazyadl
...stify;">Peach rosette mosaic virus (PRMV) was detected from naturally infected commercial peach trees growing under field conditions at El Dakahlia Governorate, using DAS-ELISA. The detected virus is recorded for the first time in Egypt. Virus identification was performed using symptom expression. host range. graft transmission. serological tests i.e., DAS-ELISA, TBIA and DBIA, physical properties, electron microscope (EM) and reverse transcription Polymerase ...
A-New-Whitefly-Transmitted-Geminivirus
...her one showed yellow mosaic symptoms on tomatoes, named Tomato yellow mosaic virus (TYMV)-Qalyubia isolate. The genetic diversity at the molecular level for the two isolates showed that the coat protein and the replicase genes of putative TYMV-QaIubia are not identical to TYLCV resembled genes, at least at the flanking regions of each gene. According to the results obtained from the PCR products and indicator host plants, i...
M.A. Abo-EInasr l, Kh.A. EL-Dougdougl, M.H. El-Kattan2 and L.A. Salem2
...ainst Zucchini yellow mosaic Potyvirus (ZYMV). These chemicals were. Potassium sulfate, Magnesium sulfate, Ammonium sulfate, Chelated iron, Chelated zinc, Chelated manganese, and salicylic acid. SAR was determined via disease severity. virus concentration and some biochemical changes i.e., the high level of endogenous salicylic acid, proteins related to inducers and activity of peroxidase and chitinase enzymes. Salicylic acid (0.5%) and potassium sulfate (3%) ...
E.F. Mohamed1 and I.M. Sabra2
...ign: justify;">Tomato mosaic disease caused by Tomato mosaic (ToMV) is widespread wherever tomato is grown ToMV affects plants and yields. Virus was isolated and identified on the base of symptomatology on tomato plants, host range, diagnostic hosts, physical ptoperties, mode of transmission, and ELISA detection. ToMV had a longevity in vitro (LIV) of 90 days at room temperature. dilution end point (DEP) Of 10-6 and thermal ...
E.F. Mohamedl and A.A. Owayss2
...ced the occurrence of mosaic symptoms on Faba bean plants to 80, 70, 60, 75 and 90% of infected plants immediately: to 75, 65, 45, 55 and 80% after one hour storage period and to 65, 50, 35, 45 and 70% after two hours. when used at a concentration of l, 3, 5, 10 and 20% respectively. In vivo experiment, using of propolis immediately pre-inoculation was more effective in reducing the occurrence of symptoms in infected plants than post-inoculation. The same resu...
M. Osman1, Kh. El-Dougdoug2, E. T. Abd El-Salam3, R.M. Taha4 and R.M. El-Hamidl
...n isolate of Cucumber mosaic cucumovirus (CMV) infected cucumber plants was detected by DAS-ELISA and confinned by inoculation of Chenopodium amaranticolor. CMV-isolate was propagated on squash plants var. Eskandarani. After 15 days. systemic symptoms appeared in the newly formed leaves in the form of vein banding, severe mosaic, yellowing, malformation, and leaves wilting. Histopathological studies of CMV-infected squash le...
Thobela Louis Tyasi*, Lebo Ngorima, Victoria Rankotsane Hlokoe
