Search for Bacteriophage

Three Bacteriophages SA, SA2 and SNAF can Control Growth of Milk Isolated Staphylococcal Species

Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*

...astitis. In ongoing era, bacteriophages are an alternate possible effective remedy. Therefore, in this study the infection ability of three lytic phages SA, SANF and SA2 was determined against ten isolates of Staphylococci and one Micrococcus, isolated from five raw milk samples. Their morphological, biochemical characterization and 16S rRNA sequencing indicated that two strains were S. aureus, while all others were different coagulase negative Staphylococci. ...

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives

... amplification using the bacteriophage 29 DNApolymerase allows for a reliable diagnosis of geminiviruses and presumably all viruses with small single-stranded circular DNA genomes. The results show the efficiency of this technique in characterizing viral DNA components of several geminiviruses from experimental and natural host plant sources. Nucleotide sequence data offers identification of viral molecule to strain/species level for accurate diagnosis....

Storage of Bacteriophages at 4°C Leads to no Loss in Their Titer after One Year

Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*

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Stability of Salmonella typhimurium Bacteriophage to Some Physical and Chemical Factors

Abeer A. Feisall; B.A. Othman2; Kh.A. El-Dougdoug2 and H.S. Shalaby1

...8 kbp.

...

Abundance and Variation of Virulence of Rhizobiophages Isolated from the Rhizosphere of Some Legume Plants in Egypt

Ramy A. Qabel1; Tarek F. El-Arabi2; Adel A. Shoukry1; Hassan H. Elsebaay1; Abdel-Aziz F. El-Hamahmy1

...d affect the rhizobia is bacteriophage infection. In this context, this study aimed to investigate the abundance of rhizobiophages in Egypt. Rhizobiophages were isolated from the rhizosphere of different legume plants. While no temperate phages were detected, two groups of phages were determined based on their virulence. Highly virulent phages designated as group V showed clear plaques and were able to infect many of Rhizobium species and strains. The other gr...

Isolation of Pseudomonas aeruginosa Phages from Wastewaters

Stephen Chijioke Emencheta¹, Chinelo Charity Eze¹, Anthony Amaechi Attama², Damian Ejike Agbo¹ and Ebele Benedette Onuigbo¹*

...st the lytic action of a bacteriophage specific to Pseudomonas sp. Both Pseudomonas spp. and bacteriophages were isolated from residential wastewaters. The isolated Pseudomonas spp. were confirmed through biochemical tests. Antibiogram was done with nine (9) different antibiotics, including;aztreonam (ATM), chloramphenicol (CPL), gentamicin (GTN), tetracycline (TTE), sulphamethoxazole/trimethoprim (SXT), amoxicillin/clavulan...

Isolation of Escherichia Coli Phages from Waste Waters

Stephen Chijioke Emencheta¹, Anthony Amaechi Attama², Ezinwanne Nneoma Ezeibe¹, Adaora Angela Agubata¹ and Ebele Benedette Onuigbo¹*

...st the lytic action of a bacteriophage specific to Escherichia coli. Both Escherichia coli and bacteriophages were isolated from farmlands and residential wastewaters. The isolated Escherichia coli were confirmed through phenotypic and biochemical tests. Antimicrobial susceptibility test to nine antibiotics was determined. Multi-antibiotic resistance index (MARI) was assessed. Spot assay was done to determine lytic action of...

Application of Phages to Control Escherichia coli Infections in Native Noi Chickens

Luu Huynh Anh¹, Huynh Tan Loc², Nguyen Hong Xuan³, Le Minh Thanh¹, Trinh Thi Hong Mo⁴, Ly Thi Thu Lan⁵, Nguyen Trong Ngu¹*

Effect-of-Bacteriophages-on-Intestinal-Colonization-of-Escherichia-coli-Cecal-Microbiota-Composition-Intestinal-Morphology-and-Growth-Performance-in-Nursery-Pigs-from-Commercial-Pig-Farms

Pawinee Kingkan1, Thanathip Supcharoenkul1, Chaowit Rakangthong1, Chaiyapoom Bunchasak1, Komwit Surachat2,3, Wiriya Loongyai1*

...termine the effects of a bacteriophage cocktail on growth performance, intestinal morphology, E coli detection, cecal bacterial composition, and the incidence of diarrhea in nursery pigs for 6 weeks. A total of 800 pigs (Large White × Landrace × Duroc) were randomly allocated to two treatments: a basal diet supplemented with a mixture of amoxicillin and colistin (Amox-Co) and a basal diet supplemented with amoxicillin and 1 g/kg of a

Effects of Bacteriophage Cocktail to Prevent Salmonella enteritidis in Native Broiler Chickens

Nguyen Thi Anh Thu1*, Ly Thi Thu Lan1, Nguyen Thuy Linh1, Chau Cong Dang1, Nguyen Hoang Phuc1, Tran Dinh Tuan1, Nguyen Trong Ngu2

...valuate the effects of a bacteriophage cocktail (BC) against Salmonella enteritidis (SE) in native broiler chickens. Totally, 320 native chicks were divided into two experiments using a completely randomized design consisting of 4 treatments and 4 replicates of 10 chicks per experiment. In both experiments, BC was used at a dose of 109 PFU/mL, and SE was used at 108,2 CFU/mL. In the protective experiment, 1-day-old chicks were arranged in CD) control diet; BC+...

Cocktail Phage Therapy for Bacteria Contaminating Meat in Egypt

Amany M. Reyad¹*, Aya Maher Rabie¹, Reda Mohamed Taha¹ and Khalid El-Dougdoug²

Physiological Profiling of Lytic Bacteriophages and their Efficiency against Salmonella Enteritidis on Chicken Breast Cuts

Aleena Kokab¹, Ali Ahmad Sheikh¹*, Masood Rabbani¹, Wasim Shehzad², Muhammad Ilyas Riaz¹, Sohail Raza¹ and Rida Haroon Durrani¹

...-food sector. The use of bacteriophages, as a potential replacement, for antibiotics and as food antimicrobial agents to potentially target foodborne pathogens is gaining augmented attention. This study’s goal was to isolate and physiologically characterize lytic bacteriophages against Enteritidis, and to ascertain their efficacy in modulating bacterial population on chicken breast cuts in the form of a phage cocktail....

Virological Evaluation of Drinking Water in Egypt

Azzami, Mohamed 1.; Hakiml , Aya Salah El-Din; Othman2, Badawi A. and 2 El-Dougoug, Khaled A.

...ail with full heads, the bacteriophage resembles those belong to the Myoviridae family. Enteroviruses were detected using RT-PCR and real-time-RT-PCR in inlet water of El-Bagour and Shibin ElKom stations in summer season, while H5N1 was not detected in all sites throughout four seasons. The viruses obtained by ultrafiltration process were negatively stained with 2% phosphotungestic acid. Electron microscopy revealed that human virus particles were of 24-30 nm ...

Isolation and Characterization of a Pseudomonas aeruginosa Bacteriophage from Agriculture Drain Water

Amina A. II. and M.K. Amin

...xt-align: justify;">Five bacteriophages infecting Pseudomonas aeruginosa strain were isolated from agricultural drain water of Zagazig city. One of them designated as PAI has been characterized. The ability of phage PAI to form plaques with strain PAOI was examined. Two kinds of plaques (turbid and clear) have been observed. The average number of turbid plaques was 2.52 x 109 pfu/ml whereas of clear plaques was 9.96 x 107 pfu/n) l. The phage was able to lysis ...

Isolation and Characterization of a Pseudomonas Putida Bacteriophages

Mayada A. Abd Elgalell; B.A Othman2; Th. Radwan, 1 and Amal S.M. Abo-Sinna3

Evolution of super-drug resistant microbial strains: mechanisms and strategies for containment

SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3

...as, probiotics, CRISPRs, bacteriophages, nanotechnology and phytochemicals.

...

Biologia (Lahore)

Featuring

Quantification of Sodium from Food Sources by Using Various Analytical Techniques

Nauman Jamil Khan, Rabia Tariq, Hina Saleem and Muhammad Waheed Mushtaq

Biologia (Lahore), Vol.69, Iss. 2, Pages 66-75
Studies on the Occurrence of Prostate Cancer in Gujrat, Punjab, Pakistan and its Association with Testosterone Level

Kanwal Nisa, Sadia Roshan, Shazia Shamas, Raheela Atta Mustafa, Shamaila Irum, Kalsoom Sughra and Memoona Iqbal

Biologia (Lahore), Vol.69, Iss. 2, Pages 54-60
Prevalence of Gastro-Intestinal Parasites in Pigeons of District Jhang-Pakistan

Muhammad Fiaz Qamar, Tahir Hussain, Iram Liaqat, Madiha Kiran, Abdur Rahman Ansari, Faiza Yasmeen and Tahira Batool

Biologia (Lahore), Vol.69, Iss. 2, Pages 48-53
Phytochemical Screening and In Vitro Antioxidant Activity of Extracts of Ipomoea purpurea Leaves from Iran

Fatemeh Beheshti, Maliheh Safavi, Mohammad Reza Akbari Eidgahi, Parviz Kokhaei, Mehdi Vazirian and Ali Akbar Shabani

Biologia (Lahore), Vol.69, Iss. 2, Pages 32-39