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Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*

 

...xt-align: justify; ">Two citrus viroids, Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd), have been reported decades ago without further pathological and genetic researches in Taiwan. Here, bioassay, multiplex RT-PCR and multiplex real-time RT-PCR were used to detect the current status of the two viroids showing HSVd was more prevalent than CEVd and simultaneous infections were common in field. Uneven distribution...

Nadeem Khan1*, Arshad Ali Khan1, Mukhtar Ahmad2, Muhammad Nouman2 and Badshah Islam1

Muhammad Aslam Shahid, Muhammad Iqbal and Muhammad Niamatullah

... crop of Pakistan, after citrus and mangos. Artificial pollination is a one of most important cultural practice for improving yield of Date palm due to diocious nature of palm tree. Pollen sources play important role in fruit setting .The growers pollinate the date by using low potency pollen source and get low yield. Present studies were conducted with the aim to explore best pollen sources for maximum fruit set and yield. In this respect, an experiment was c...

Waqas Ahmed Dogar2, Arshad Ali Khan3*, Saeed Ahmed2, Sudheer Tariq2, Mukhtar Ahmad2, Muhammad Imran2, Muhammad Noman2 and Nadeem Khan1 

...n on growth and yield of citrus (Kinnow and Musambi) grown under drip irrigation at Postgraduate Agriculture Research Station (PARS) Institute of Horticultural Sciences, University of Agriculture Faisalabad. Plants were grown at three planting distances as T1 (11 x 22ft), T2 (11 x 11ft) and T3 (22 x 22ft). T1 (11x22ft) was optimum plantation and showed best results for yield. Maximum fruit yield per plant (3.6373 kg) was observed from the same plants. T2 (11 x...

K.N. Ahmed and M.R. Hasan

Sudden outbreak of mealy bug and armoured scale causing severe damage to economic crops in Bangladesh
...r, papaya, basil etc. by citrus mealybug, Planococcus citri (Risso) (Homoptera: Pseudococcidae) has been noticed in Bangladesh. It is mainly a pest of citrus and coffee crops but now it has become polyphagous in nature. Based on laboratory studies on immature and mature fruits of lady's finger; the total period of all four nymphal stages lasted for 32.6 ± 4.17 days at an average room temperature of 28 ± 2º...
Azhar Abbas Khan1,*, Arif Muhammad Khan2 and Muhammad Afzal3
...he fresh leaves of rose, citrus, brassica and wheat (1.2±0.2006, 1.3556±0.1416, 1.5778±0.1829, 2.1444±0.2027, respectively) attracted significantly more numbers of C. septempunctata as compared to control and aphids alone (0.7667±0.1204 and 0.8333±0.1153). With every next hour (3, 6 and 12 h) of observation the movement of C. septempunctata toward target localities was elevated. Finding of the current inv...

Furukh Faiz1,2, Muhammad Issa Khan2, Masood Sadiq2 and Haq Nawaz3

... the current studies the citrus waste is dried and mixed with the broiler feed replacing maize from the feed at 0%, 2.5%, 5%, 7.5% and 10%. 225 birds were reared in 5 treatments and 3 replications of each treatment having 15 birds in each replication. The chicks were being provided with the standard conditions for light, water, vaccination and experimental feed for 42 days. The body weight gain, feed intake and the feed conversion ratio was observed weekly. Af...
Mazher Abbas1, Irfan Mahmood1*, Arshed Bashir1, Tahir Mehmood2, Khalid Mahmood1 and Zubair Ikram1
... adoption of recommended citrus production practices and to investigate the factors that can affect the adoption of recommended citrus production practices. A farm level survey was conducted in Sargodha and T.T. Singh districts to gather the information about the awareness and adoption of recommended production practices of citrus. The study sample was consisted of 140 respondents. The bin...

Raza Ullah1*, Qaisar Shah Safi2, Ghaffar Ali3 and Irfan Ullah

...ages in the marketing of citrus. This study is an attempt to examine the marketing margins of the intermediaries involve in the value chain of sweet oranges of citrus group. Three villages in Bunir district are randomly selected and a total of 120 sampled respondents are selected using proportional allocation method. The findings revealed that majority of the farmers sold their orchards to the pre-harvest contractors. The mo...

Abbas Ullah Jan1, Muhammad Fayaz1*, Yousaf Hayat2, Dawood Jan3 and Ghaffar Ali3 

...ruits, apple, banana and citrus with increase in household income for urban households. For rural households with increase in household income, quantity elasticity of all the fruits tends increase with the exception of grapes where it decreases and remains constant in dates. The estimated quality elasticities reveal that both the urban and rural households purchase higher quality fruits as their income rise and are willing to pay a higher price for enhanced qu...

Fazal Ullah1*, Muhammad Sajid1, Syeda Leeda Gul2, Bibi Zainub1 and Maaz Khan

Justyna Batkowska1,*, Lukasz Wlazlo2, Kamil Drabik1, Bozena Nowakowicz-Debek2, Karrar I.A. Al-Shammari3 and Magdalena Gryzinska1

 Ayesha Tahir*

FORECASTING CITRUS EXPORTS IN PAKISTAN
...ity and export value for citrus in Pakistan. The findings of the study are based on citrus export quantity and export value data from 1990 to 2011. Two models of trend analysis were applied but most appropriate model for trend analysis of the present study was quadratic trend model. Forecasting was done from 2012 to 2016.The forecasted export quantities are 1643.13, 1854.03, 2077.80, 2314.46 and 2564.00 thousand tons and for...

 Abdul Haq*, Anjum Shehzad**, Muhammad Ilyas**, Muhammad Ishaque Mastoi**, Abdul Rauf Bhatti** and Mian Inayatullah*

DIVERSITY AND RELATIVE ABUNDANCE OF CITRUS POLLINATORS IN DISTRICT HARIPUR, PAKISTAN
... 3094 specimens of adult citrus pollinators collected from district Haripur represented four orders of insects i.e., Diptera with 16 species, Hymenoptera with 14 species, Lepidoptera with 3 species and Coleoptera with 2 species. The calculated values of relative abundance showed that is most abundant species in ecosystem followed by sp. and . All calculated values of diversity indices except Shannon-H does not show significant difference. Shannon's diversity s...

 Mubbashira Nazir*, Saira Batool*, Sultan Ali Tariq**, Abid Hussain** and Ya Sakina**

INTERDECADAL GROWTH VARIABILITY AND DECOMPOSITION ANALYSIS OF FRUIT CROPS IN PUNJAB, PAKISTAN
...havior for major fruits (citrus, mango and guava) in almost all the decades, except for mango in 1970s and guava in 2000s. The decline in production of mango in 1970s was due to negative growth in both area and yield. While, a slowdown growth in the area and decrease in yield were reasons of decline in production of guava in 2000s. Growth in production of minor fruits showed a cyclical trend i.e., increased and decreased in alternate decades. Results of Cuddy-...
Muhammad Khalid Mukhtar1,*, Hummaira Iqbal1, Hafiz Muhammad Tahir2, Haseena Ghulam Muhammad1 and Muhammad Irfan1
...tudy were collected from citrus orchards of Sargodha and maintained individually in the laboratory. Both the control and insecticide treated spiders were offered larvae of Drosophila melanogaster in different densities. C. citricola increased prey consumption as the prey density was increased. Insecticide treated C. citricola consumed less prey as compared to control. Effects of chlorpyrifos on the prey consumption of C. citricola w...

Muhammad Fiaz1*, Muhammad Afzal1 and Muhammad Zeeshan Majeed1,2 

...6 and 2017 against Asian citrus psyllid Diaphorina citri, one of the economic insect pests of citrus plants and a putative vector of Huanglongbing worldwide. Treatments were comprised of label-recommended (FD) and its half (HD) dose rates of spirotetramat and I. fumosorosea formulations. Both treatments were applied either alone or in binary combinations. There was a significant reduction in psyllid population for all treatm...
Matiyar Rahaman Khan1,*, Sabayasachi Pal2, Amit Singh2, Ashok Dhananjaybhai Patel3, Bhagubhai Ambaram Patel3Tushar Manohar Ghule2 and Victor Phani1
.../div>...
Habib-ur-Rehman1*, Saima Mirza1*, Mansoor-ul-Hasan2, Qurban Ali3, Hafiz Abdullah Shakir4, Muhammad Yasir5

Ashraf Khan1*, Suliman Shah2, Maid Zaman3 and Komal Habib2

...t-align: justify;">Asian citrus psylla, Diaphorina citri Kuwayama, is considered to be the most noxious pest of almost all species and varieties of citrus and its infestation found throughout the world. It is becoming serious threat to the brightening future of citrus growing areas of Pakistan. Thirteen different insecticides were usedfor effective control against ...

Shumaila Nazeer1, Iqbal Javed1*, Allah Bakhsh2, Mudassar Yasin2, Haroon Javaid3 and Kaniz Fatima1 

...;">Brazil is the largest citrus producing country in the world. Pakistan stands at the number 12th among citrus producing country. Pakistan is the world largest producer of citrus reticula variety (kinnow). The current study under hand is conducted to find the margin and determinants of kinnow exports from Pakistan to international markets of UAE and USA by using the primary data. Primary ...
Zhiwen Zou, Jianfei Xi, Fen Chen, Rui Xu, Tianrong Xin and Bin Xia*
...fy phytoseiid species in citrus.
...
Hafiz Abdullah Akbar, Zafar Iqbal, Waqas Raza*, MuhammadUsman Ghazanfar and Salman Ahmad
...ium digitatum causes citrus green mould by using 7 plant extracts alone and in combination with wax and 3 concentrations of salicylic acid alone and in combination with wax. The study revealed that in case of plant extracts maximum growth inhibition (35.90 %) was shown by Anar followed by Chabir (33.60%) and Garlic (29.16%), while minimum growth inhibition was recorded from Parthanium (17.43%) and Ginger (19.94 %). In case of plant extracts + wax maximum g...
Muhammad Arshad1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Yasir Iftikhar2, Samina Khalid3, Zahoor Hussain4, Jaime Molina-Ochoa5,6 and John E. Foster6
...structive insect pest of citrus nurseries as well as orchards in Pakistan. The aim of this study was to assess the effectiveness of some synthetic insecticides and essential oils against citrus leafminer larvae. Two laboratory bioassays, leaf dip bioassay (LDB) and topical bioassay (TB) were developed to check the efficacy of tested chemicals and essential oils. As seen from results, abamectin showed significant mortality (6...

Huma Khalil1*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1, Muhammad Sajjad Khalil1, Farghama Khalil2 and Hafiz Khurram Shurjeel1 

... braconid parasitoids in citrus orchards of Sargodha, Pakistan. Surveys were done during January 2014 to December 2015 from various citrus growing localities. A total of 3,176 parasitoids belonging to five subfamilies (Alysiinae, Aphidiinae, Braconinae, Microgastrinae and Opiinae), 12 genera and 16 species were collected. Out of them, two genera and 12 species were recorded for the first time from this area. Aphidiinae conta...

Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Luqman and Muhammad Zeeshan Majeed 

... the emerging threats to citrus industry in Pakistan is the infestation of mealybug Drosicha mangiferae (Pseudococcidae: Hemiptera), a damaging insect pest of a large number of agricultural and horticultural crops in South East Asia. In spite of extensive applications of conventional synthetic pesticides, citrus farmers are unable to get rid of this pest. In order to find out alternate biorational pesticides, this laboratory...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Asam Riaz and Muhammad Zeeshan Majeed
...cultural crops including citrus. Extensive and injudicious use of conventional synthetic insecticides against D. mangiferae have led to many environmental and health problems urging to seek out environment-friendly and safe alternate strategies to control D. mangiferae. To this end, the present study evaluated the methanolic extracts and essential oils of eight indigenous plant species for their insecticidal potential against 2nd insta...
Muhammad Saeed1,2,*, Tariq Mukhtar1 and Malik Abdul Rehman3
...e population dynamics of citrus nematode (Tylenchulus semipenetrans) in a production area is required for the assessment of damaging potential of the nematode to citrus, identification of key factors that influence population densities, and to devise effective management strategies. In the present study, seasonal fluctuations in the population densities of T. semipenetrans were studied in two
Hafiza Mehwish Iqbal1, Shahid Yousaf1, Salman Khurshid1*, Qurrat Ul Ain Akbar1, Saqib Arif1, Nasreen Fatima2 and Ali Murad Rahoo3
...to assess the quality of citrus in terms of postharvest physiological disorders and organoleptic properties in relation to the occurrence of pathogenic diseases. Fruit samples (injured and healthy) were taken from the three different local markets of Karachi. Some of the fungal species viz. Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Rhizopus stolonifer and Alternaria alternata have been identified in citru...

Muhammad Arshad1, Muhammad Irfan Ullah1*, Muhammad Afzal1, Mian Anjum Murtaza2, Ejaz Ahraf3, Zahoor Hussain4, Syed Muhammad Ali Zahid1 and Maryam Riaz1 

...s insect pests attacking citrus plants in Pakistan. The study was conducted to quantify the leaf area damage of eight citrus cultivars caused by mining activity of CLM during summer 2016. The total leaf area and mine area per leaf were calculated by the image analysis method using Sigma Scan Pro 5.0 software. The results of the present study showed that CLM generated larger mines; 1.64 cm2 on Grapefruit, 1.44 cm2 on Kinnow a...
Sabbah M. Allam, T.M. El-Bedawy, M.H. Bakr and A.E.M. Mahmoud*
...to examine the effect of citrus by-products on presence of fecal E. coli and Salmonella species, and on antioxidant biomarkers.
...
Rakhshanda Kousar1, Tahira Sadaf1*, Muhammad Sohail Amjad Makhdum2, Muhammad Amjed Iqbal1 and Raza Ullah1
...ee fruits, namely mango, citrus and dates by using Revealed Comparative Advantage, Revealed Symmetric Comparative Advantage, Relative Export Advantage Index and Log of Relative Export Advantage Index. For this purpose, time series data has been collected for the period 2001-2018 from Food and Agricultural Organization Statistics, International Trade Centre map, and World Banks’ World Integrated Trade Solutions, Trade Development Authorities of Pakistan, ...

Yousaf Noor*, Zahir Shah and Muhammad Tariq 

... and yield attributes of citrus (Citrus Sinensis) orchards established on calcareous soils at three farmers fields in Dubandai and Palai area of District Malakand of Khyber Pakhtunkhwa, Pakistan during 2009-2010. A randomized complete block experiment with split plot arrangement was used. Methods of application (Soil and Foliar) were assigned to main plots while micronutrients (Zn @2.5 kg ha-1, B @ 2.5 kg ha-1 and Zn plus B)...
Sumaira Abbas1, Muhammad Sultan Haider2,*, Fatima Kafayet1, Sana Ashraf2, Atifa Masood3 and Moazma Batool4
.../sub>, were prepared and citrus peel powder was added @ 200g, 400g and 600g/ 1000g, respectively. While fourth one, control diet (To) was without citrus peels. These is nitrogenous diets (30% protein) were offered to Carassius auratus juvenile having body weight 20±7.54g in powder form for 92 days. Weight gain, length gain and FCR were calculated fortnightly. At completion of feeding trial, color int...

Awais Ahmed Khan1*, Zafar Iqbal1 and Muhammad Atiq2 

...to control green mold of citrus caused by the Penicillium digitatum. Antifungal activities of organic elicitors were evaluated and all the elicitors expressed significant results. Salicylic acid was the most effective to control the disease and showed minimum mycelial growth diameter (35mm) followed by benzoic acid (44mm), jasmonic acid (45.5), and ascorbic acid (50mm) as compared to control (92mm) treatment. Organic elicitors activated the defense system, by ...

Muhammad Ramiz Murtaza1, Tahir Mehmood2*, Aziz Ahmad2 and Uzma Arshad Mughal3 

Asif Yaseen1, Muhammad Israr2*, Allah Bux Khan1, Ahsan Javed3, Nafees Ahmad4 and Zia Ullah

...hannels in the Pakistani citrus industry. The study employed survey technique to collect data from 300 citrus farmers in Punjab, and multinomial treatments effects were used to analyze the data. The results indicated though participation in traditional marketing channels (with intermediaries) is still profitable, high value marketing channels appeared more attractive alternative, having been shown to offer increased net inco...

Saleem Ashraf1, Raheel Saqib2*, Zakaria Yousuf Hassan3, Muhammad Luqman4 and Abdur Rehman5  

...nsistent supply chain of citrus in Pakistan. This research aimed to bridge the research gap by soliciting intermediaries’ role in the citrus marketing process. Total 120 randomly selected citrus growers were interviewed face to face and collected data were analysed using Statistical Package for Social Sciences. Findings affirmed that middleman, processing factories and friends/relati...
Muhammad Irfan Ullah1*, Muhammad Arshad1, Sajjad Ali2, Asad Abdullah1, Samina Khalid3, Hafiz Muhammad Aatif4, Syed Muhammad Ali Zahid1, Muhammad Afzal1 and Jaima Molina-Ochoa5
...e infected leaves due to citrus canker were also tested using BioLeaf for foliar analysis. Our results demonstrated that the quantification of foliar damage using BioLeaf application was easy and accurate. This method is also comparable with other techniques like digital analysis by ImageJ software and leaf area meter devices. With these results, it is concluded that the BioLeaf application has resulted in an accurate leaf area measuring tool of foliar damage ...
Muhammad Yahya1, Muhammad Afzal1, Muhammad Zeeshan Majeed1*, Imtiaz Sarwar1, Khurram Shehzad2, Muhammad Luqman3 and 
Sher Muhammad Shahzad2
...ped and non-intercropped citrus and guava orchards, natural grassland, bare land and wetland peripheries) using population abundance and dynamics of edaphic springtails (collembola) and mites (acari) as bioindicators. Using metallic soil corer (10 cm length and 10 cm diameter), extensive random soil sampling was carried out from selected localities in district Sargodha (Punjab, Pakistan) and soil microarthropods (i.e. springtails and mites) were extract...

Akbar Hayat*, Muhammad Nawaz Khan, Ehsan Ul Haque, Ahmed Raza, Faheem Khadeeja 

...ance of different exotic citrus root stock was studied at Citrus Research Institute Sargodha from 2013-18. The trail was conducted according to RCB design with three replications. The Five Treatments four exotic and one local root stock (To Rough lemon, T1 Troyer citrange, T2 Cox mandarin, T3 C-35, T4 Carrizo citrange) were budded with Feutrells early in 2013. The statistical analysis of vegetative data from trial site shown...

Hafiz Abdul Ghafoor1*, Muhammad Afzal1, Muhammad Luqman2, Muhammad Arshad Javed2, Syed Wasim Hasan3 and Muhammad Zeeshan Majeed

...en an emerging threat to citrus industry in Pakistan and is considered as hard-to-control pest. This study evaluated some selected pest control options against the infestation of D. mangiferae under field conditions. The treatments included some most effective conventional and novel chemistry insecticides, botanicals and cultural control practice (i.e. two under-canopy deep hoeings with 15 days interval along with lower-branch pruning) alone or in combination ...

Ehsan-Ul-Haque1, Akbar Hayat1*, Muhammad Asim1, Sajjad Hussain2, Muhammad Shakeel Hanif3, Muhammad Zubair1, Muhammad Abdullah Jamil1 and Faheem Khadija1 

...n to control postharvest citrus fruit decay with perks of safety and convenience. 

...

Ummad ud Din Umar1, Syed Atif Hasan Naqvi1*, Ishfaq Ahmed1, Ateeq ur Rehman1, Muhammad Asif Zulfiqar2, Sibghat Ullah3, Shah Pasand4, Zehri Khan3 and Abdur Rehman5

Prevalence and Serological Detection of Spiroplasma citri, a Cause of Citrus Stubborn Disease in Southern Punjab, Pakistan
... responsible for the low citrus production in terms of quality and quantity but also moving the citrus industry to down fall in citrus growing areas throughout the world. Current research was organized to study the distribution of disease using the serological based detection methods by conducting a comprehensive survey in various districts of Southern Punjab. The results of the survey and...

Malik Abdul Rehman1, Shafqat Ali1, Muhammad Babar Shahzad Afzal1, Muhammad Nawaz Khan1 and Mujahid Ali2*

Efficacy of Chemicals and Botanical Extracts to Control Citrus Canker on Kinnow in Sargodha Region
...y was carried out at the citrus farm of the Citrus Research Institute, Sargodha, Punjab-Pakistan during 2011-13 on ten-year-old bearing trees of Kinnow plants. There were nine treatments, control (no chemical was applied), Neemsol @ 5 ml, copper oxychloride @ 3 g, streptomycin sulphate @ 1 g, potassium acetyl benzoic acid @ 10 ml, difenoconazole @ 0.5 ml, extracts of Acacia nilotica @ 10 ml, Datura alba @ 10 ml, and Allium c...

Malik Abdul Rehman1*, Hafiz Muhammad Ehsan2, Tehseen Ashraf2, Zulfiqar Ali Gurmani3, Sajjad Khan3 and Mujahid Ali2

Effect of Growing Media on Plant Growth of Rough Lemon (Citrus jambhiri Lush.) and Poncirus (Citrus trifoliata)
Muhammad Zeeshan Majeed1*, Muhammad Shahzad Akbar1, Muhammad Afzal1, Muhammad Mustaqeem2, Muhammad Luqman3, Ijaz Asghar4 and Muhammada Asam Riaz1
Muhammad Zeeshan Majeed1*, Muhammad Afzal1,Muhammad Asam Riaz1,Kanwer Shahzad Ahmed1, Muhammad Luqman2, Mehar Zubair Shehzad1, Muhammad Bilal Tayyab1, Mujahid Tanvir1 and Saadia Wahid1
... evaluated against Asian citrus psyllid (Diaphorina citri), armyworm (Spodoptera litura), house mosquito (Culex quinquefasciatus) and subterranean termite (Odontotermes obesus) using twig-dip, leaf-dip, aqueous exposure and filter paper-dip bioassay methods, respectively. Results revealed that the extracts of Mentha longifolia, Sonchus asper and Nerium indicum were the most toxic to D. citri exhibiting 90...

Muhammad Iqbal1, Muhammad Ehetisham ul Haq1*, Muhammad Kamran1, Muhammad Idrees1, Shahid Nazir2, Ihsan Ullah3, Sumera Naz1, Shaukat Ali1 and Muhammad Zafar Iqbal2

Morpho-Molecular Characterization of Xanthomonas Axonopodis Pv. Citri Associated with Kinnow (Mandarin) and its Management
...a reliable estimation of citrus canker disease in District Sargodha of Punjab Province. Nine sampling sites showed 90 % the presence of Xanthomonas axonopodis pv. citri and only one site was free of citrus canker disease. Total cellular DNA was isolated from freshly growing aqueous bacterial culture using CTAB protocol with modifications. Amplification of 581bp fragment from isolated DNA from bacterial isolates confirmed the...

Muhammad Shahzad Akbar, Farrukh Sajjad, Muhammad Afzal, Muhammad Luqman, Muhammad Asam Riaz and Muhammad Zeeshan Majeed*

Field Evaluation of Promising Botanical Extracts, Plant Essential Oils and Differential Chemistry Insecticides against Subterranean Termites Odontotermes obesus (Isoptera: Termitidae)
Ashara Sajid1, Muhammad Usman Ghazanfar1, Saeed Rauf 2, Zahoor Hussain3, Salman Ahmad1 and Yasir Iftikhar1,*
...e-height: normal;">Asian citrus greening disease is one of the leading causes of the citrus decline in many parts of the world, including Pakistan. Adequate information about disease incidence is essential for the eradication and controlling measures of disease. In this context, a survey regarding the incidence of citrus green disease (CGD) was recorded in two cultivars Kinnow (
Faheem Ahmed Khan1*, Sarzamin Khan2, Qurat-ul-Ain3, Saqib Ishaq1Muhammad Salman1, Abdul Rehman1, Ikram Ullah4, Kalsoom5 and Johar Jamil5
Muneer Abbas1*, Dilbar Hussain2, Muhammad Saleem2, Abdul Ghaffar2Sohail Abbas3, Niaz Hussain1 and Abdul Ghaffar1
...onducted in three guava, citrus and mango producing districts of Punjab i.e. Faisalabad, Sargodha, Toba Tek Singh with five treatments. Each experimental unit comprised of 1 ha area. Five treatments viz. A=sanitation, B=MAT, C= protein based baits, D= plant extracts and E= A+B+C+D were used during 2015-16. Data of % infested fallen fruits, total flies/trap, total flies captured/year, % fruit punctures, pupal population, % fruit infestation and ma...

Muhammad Afzal1, Muhammad Irfan Ullah1*, Muhammad Hamid Bashir2, Shah Najaf Mukhtar1, Muhammad Arshad1, Nimra Altaf1

...iodiversity in different citrus orchards of Kinnow, Musambi, and Feutrell’s early at tehsil Sahiwal Sargodha, Sillanwali, and Bhalwal of district Sargodha. Sampling was done at fifteen days interval to record the population dynamics and identification of citrus mites during 2018. A total 37,963 mites specimens were collected in which nine species were identified from four families Cunaxa sp. (Cunaxidae); Amblyseious an...

Ghulam Murtaza1*, Muhammad Ramzan2, Assad Ullah3, Abid Ali5, Ayesha Zafar3, Rukhsar Beanish3, Ahmad Ali4, Ghulam Mustafa4 and Mudassar Aslam4 

..., apple, fig, banana and citrus were exposed to Bactrocera zonata for oviposition. Fruits were placed in cage to assess the host preference for oviposition fruits having equal weight (500g). Fifty pairs of 11-12 days old adult of B. zonata were released in cage for 24h to determine their oviposition. The study resulted that maximum number of pupae (125.34) were formed in guava fruit exposed to B. zonata followed by banana (104.12), cit...

Elharony, S.B.1,3, SaharA.Youssef2 and Richard F. Lee3

...ansmissible pathogens of citrus including prokaryotic pathogens,
DNA and RNA viruses and viroid provides a new approach to virus
and virus like detection and identification. The method provides a
pure preparation of un-degraded RNA and DNA in high yield and
can be completed within 2 h. It is particularly useful for processing
large number of samples and for isolation of RNA and DNA from
sm...
Rasha M. Mahrous1,4, Ahmed K. El-Attar2, Ahlam A. AlWatban1, Sohair I. EL-Afifi3,
Nagwa M. Aref1,3
...s disease of
citrus with a major limiting factor for lime production. Symptoms of Witches' broom were observed on
limes in AL-Qalyubia governorate, Egypt. This study identified and classified the isolated WB
phytoplasma.
Method: Phytoplasma was isolated from lime trees exhibited Witches' broom Symptoms in ALQalyubia
governorate, Egypt by grafting on indicator (Vollka marina ) plants. The Phytoplasma w...

Nadeem Anwar1, Muhammad Luqman2*, Shaoib Nasir3, Moazzam Sabir1, Hafiz Bashir Ahmad4 and Saleem Ashraf5

...nd marketing behavior of citrus growers on their socio-economic development. This research was conducted in district Sargodha, the hub of citrus production. The findings of the study indicate regarding harvesting behavior of the farmers that they do not harvest the fruit by their own. Only a small number of the citrus growers harvest the fruit by their own. Majority of the farmers were fou...

Muhammad Irfan Ullah1*, Muhammad Arshad1, Abu Bakar Muhammad Raza1, Nimra Altaf1, Muhammad Afzal2

...t-align: justify;">Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Homoptera: Liviidae) and citrus leafminer (CLM), Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) are imperative insect pests of citrus orchards due to their relation with bacterial diseases. The objective of this study was to evaluate the effectiveness of soil-applied insecticides against these insect p...

Hamza Tariq1, Nasir Ahmed Rajput1*, Muhammad Atiq1, Shahbaz Talib Sahi1, Abdul Rehman1, Abdul Rashid1, Muhammad Ahsan Khan2, Akhtar Hameed1,3 and Warda Modassar Saddique1

...most damaging disease of citrus. In the present study, twelve varieties of citrus were screened against gummosis under natural field conditions under Randomized Complete Block Design (RCBD). Among these varieties, Kumquat expressed a highly resistant response with lesion size (0.750 cm), Sweet Lemon exhibited resistant response with (1.616 cm) lesion size, Mangal Singh moderately resistant with rating (3) while two varieties...
Muhammad Bilal Tayyab1, Muhammad Zeeshan Majeed1*, Muhammad Asam Riaz1, Muhammad Anjum Aqueel2, Sylvain Nafiba Ouedraogo3, Muhammad Luqman4, Kanwer Shahzad Ahmed1 and Mujahid Tanvir1
...ll over the world. Asian citrus psyllid Diaphorina citri Kuwayama (Psyllidae: Hemiptera) and cotton aphid Aphis gossypii Glover (Aphididae: Hemiptera) are destructive sap-sucking pests of citrus and cotton, respectively. Extensive use of persistent synthetic insecticides against these pests poses issues of environmental contaminations and health hazards and suggests looking for alternate biorational plant protection measures...

Mahmoud M.A.Youssef and Wafaa M. A. El-Nagdi

...ck plastic cover reduced citrus nematode, Tylenchulus semipenetrans on navel orange and reniform nematode, Rotylenchulus reniformis on sunflower. Also, dry heat was used to control rice root nematode, Hirschmanniella oryzae on rice soil and root and wheat soil. Several investigators reported that number of galls and egg-masses of root-knot nematode, Meloidogyne incognita on roots of several plants were reduced gradually by increasing gamma irradiation doses. W...
Qazi Muhammad Noman1, Farhan Mahmood Shah1, Khalid Mahmood2 and Muhammad Razaq1*
...cant threat to mango and citrus production worldwide including Pakistan. In the present study we identified and monitored the population dynamics of fruit flies species using male attractants Attrex® (methyl eugenol 98%, evyol group) and static Spinosad® (spinosad 2% and methyl eugenol 51%, target group) in mango and citrus orchards at different locations in Multan, Southern Punjab, Pakistan.

Muhammad Zeeshan Majeed1*, Muhammad Irfan Ullah1, Dilbar Hussain2, Muhammad Luqman3, Muhammad Qasim1, Gulfam Yousaf1, Hamza Latif1 and Muhammad Zeeshan1

...pealis Lethierry), Asian citrus psyllid (Diaphorina citri Kuwayama) and subterranean termites (Odontotermes obesus Rambur). Standard twig- and filter paper-dip bioassay methods were used against sap-feeding pests and termites, respectively. Factorial analyses of variance revealed a significant impact of tested insecticidal formulations and exposure time on the mortality all insect pest individuals. The most effective differential chemistry insecticides against...

Zahoor Hussain1,2, Yasir Iftikhar3, Mustansar Mubeen3, Muhammad Zia Saleem3, Muhammad Umer Naseer3, Muhammad Luqman4, Raheel Anwar5, Faheem Khadija6 and Aqleem Abbas7

...increasing the vigour of citrus against the disease. In the present study, typical symptoms of citrus greening, such as mottled leaves, were observed on the infected trees. The infected fruits were undeveloped and partially or entirely failed in attaining natural orange fruit colour. Moreover, the iodo-starch tests revealed that the color of HLB-positive leaves was dark grey to black across the sliced surface. Afterward, the...
Dyah Roeswitawati1*, Iva Kristova1, Muhidin Muhidin1, Otto Endarto2, Manar Fayiz Mousa Atoum3,4, Irum Iqrar5,6 and Luqman Ali Shah7
 
Muhammad Faisal Riaz1, Abu Bakar Muhammad Raza1*, Muhammad Zeeshan Majeed1 and Talha Nazir2
...s observed on loquat and citrus plantations. Moreover, maximum aphid population was recorded in the 1st fortnight of March in most plantations sampled. These results demonstrate the prevailing diversity of this economically important family of pest insects and would be helpful in future studies regarding their management.
...

Muhammad Rizwan, Abu Bakar Muhammad Raza, Muhammad Zeeshan Majeed*, Muhammad Arshad

...cultural crops including citrus. Population studies are important to understand the abundance, life history and estimating extinction probability. The present study was conducted in three localities (tehsils) of district Sargodha during 2017 to determine the population dynamics of D. mangiferae nymphs and adults on citrus plants. Results showed that the average population of mealybug nymphs (9.92 individuals per branch) and ...

Judith Kiptoo1*, Daniel Mutisya2, Paul Ndegwa1, Ruth Amata3, Lucy Irungu4 and Rotich Godfrey5

...19 was conducted in most citrus growing regions in Kenya to assess the abundance, distribution and diversity of plant parasitic nematodes from different soil rhizosphere. Nematode population in 200cc of soil and 5g of roots were collected for PPNs extraction by using modified Baermann’s technique and identification by morphological features. The findings indicated that four major genera were abundant in different citrus

Muhammad Ismail1,*, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1 and Umair Abbas1 and Riaz Hussain2

...tantial economic loss to citrus produce each year in Pakistan. Farmers rely on persistent synthetic insecticides for fruit fly control. Insecticidal phytoextracts are biorational alternates to hazardous synthetic insecticides. This study evaluated the efficacy of aqueous extracts of neem (Azadirachta indica A. Juss), garlic (Allium sativum L.), ginger (Zingiber officinale Roscoe) and lime citrus (Cit...

Ghassan Zahid1, 2*, Sara Iftikhar3, Muhammad Umer Farooq4 and Shakeel Ahmed Soomro5

...y grown in Pakistan like citrus, apples, mangoes, guavas, bananas, date palms and peaches, etc. Furthermore, by offering a piece of scientific information and addressing developments, notably in DNA-based molecular markers, this review paper may serve as a road map for obtaining high-yielding fruit varieties in Pakistan.

...

Akbar Hayat1*, Marayam Nasir2, Ehsan-Ul-Haque1, Muhammad Asim1, Sohaib Afzaal1, Shagufta Naz3, Muhammad Nawaz1, Abid Mahmood4, Faheem Khadija1, Fiaz Hussain1 and Tariq Mehmood5

...e nutritional quality of citrus fruits primarily depends upon the selection of rootstock. Kinnow ‘Mandarin’ is a major citrus cultivar being cultivated in Pakistan. The current study aimed to investigate the nutritional status and growth and yield of Kinnow mandarin grafted on four overseas and two indigenous rootstocks in Pakistan. Experiments were conducted at experimental station of Ci...

Muhammad Rizwan, Abu Bakar Muhammad Raza*, Muhammad Zeeshan Majeed and Muhammad Arshad

...nt fruit crops including citrus. A potential strategy to be fitted in integrated management of mealybug would be the use of phytoextracts having insecticidal activity. The main objective of this study was to find out the toxic effect of various plant extracts against D. mangiferae. Three concentrations (i.e. 6.25, 12.5 and 25.0%) of leaf extracts of neem, Azadirachta indica A. Juss (Meliaceae), eucalyptus Eucalyptus camaldulensis Dehnh. (Myrtaceae), datura Dat...

Asim Ali Khan1, Muhammad Ahmad Zeshan1, Yasir Iftikhar1*, Mustansar Mubeen1, Maham Sohail1, Sonum Bashir1, Malik Abdul Rehman2 and Muhammad Usman Ghani3

...eza disease is caused by citrus tristeza virus (CTV) expresses salient symptoms like stem pitting, yellowing of leaves, stunted growth of large, small lateral branches and twigs. The study was carried out to quantify the spatial and temporal distribution of CTV disease in major citrus growing areas of Sargodha district (Sargodha, Kot Momin, and Bhalwal). The samples from the declined orchards were collected based on symptomo...

Habib Ullah Habib1, Malik Muhammad Akram2, Mujahid Ali1*, Tahir Mehmood3, Muhammad Manzoor4, Maqsood Ahmad3, Hasseb Ahsan1, Muhammad Mazhar Iqbal3, Malik Abdul Rehman5, Muhammad Mohsan1 and Ahsan Mohyo ud Din6 

Akbar Hayat1*, Ehsan Ul Haque1, Marayam Nasir2, Rab Nawaz3, Tariq Mahmood4 and Sohaib Afzaal1

...nging to orange group of citrus was carried out at Citrus Research Institute Sargodha. The candidate varieties/cultivars were Rhode Red Valencia, Cara Cara, Amber Sweet, Sun Star and Salustiana. All of these different five citrus varieties evaluated objectively assessing their suitability both for direct consumption and value addition. The work spread over two years (2018and19) produced di...

Monis Hussain Shah1*, Rizwan Rafique2,6, Munawar Almas1, Muhammad Usman3, Sadia Yasin4 and Sajida Bibi5

Amir Abdullah Khan and Muhammad Irfan*

...rains were isolated from citrus field soil and tested for antifungal activity using the streak plate method. Among them, one bacterium was found to be antagonist against the pathogen. This was identified as Bacillus subtilis 2i through 16SrRNA. The disease causing fungus was also isolated and identified as Schizophyllum sp. B2A through 18SrRNA.The strain Bacillus subtilis 2i was cultivated in sub-merged fermentation for the production of bioactive compounds. V...

Z.A. Handoo1,†, I.K.A. Ibrahim2, D.J. Chitwood1 and A.A. Mokbel2,3

...izosphere of
citrus trees (Citrus sinensis (L.) Osbeck) with poor growth appearance and low yield from EL-Nobarria, EL-Behera
governorate, northern Egypt, during 2012-13. Dagger nematode (Xiphinema rivesi Dalmasso, 1969) was identified
on morphology of females that included female body and total stylet (odontostyle and odontophore) length, location
of guiding ring and excretory...

JAVERIA NEHAL1, ARIF MUHAMMAD KHAN1, AZHAR ABBAS KHAN*2, MUHAMMAD MUBIN3, HAFIZ MUHAMMAD TAHIR4 & JAVED IQBAL5

...quired for management of citrus canker. PCR-based diagnostic test is appropriate for monitoring pathogen in a very short time compared to laborious, non-specific and expensive protocols. ELISA, Nested PCR has been used for many years in different countries. Citrus orchards in Sargodha region were surveyed and leaf samples showing typical symptoms of citrus canker were collected. Infected s...

Imran Muhammad Siddique1, Naseem Sharif1*, Abdul Ghaffar2, Mudassar Naseer1, Ammara Noreen3, Naseem Akhtar4 and Safeer ud Din5

...ire scenario of Pakistan citrus sector, kinnow (Citrus reticulata Blanco) right from its introduction and successful acclimatization continues to dominate both in area and production besides an exportable commodity. Presence of large seed number in Kinnow is big issue for fresh consumption and as well as for processing industry in the international markets. Visualizing such compulsions of international markets, a systematic ...

Om-Hashem M. El Banaa l, A.A. Abou Zeid2, Fawzia I. Moursy3 and Azza, G. Farag

..., the causal organism of citrus stubborn disease. RecA gene protein is the enzyme primarily responsible for the homologous recombination of chromosomal DNA in bacteria. The Immunocapture- Polymerase Chain Reaction (IC-PCR) technique was applied for detection of S. citri Saglio et al in which the Spiroplasma was captured with the specific polyclonal antibodies on a solid-phase. Specific primers based on the sequence of the Morocco strains of S. citri Saglio (R8...

Muhammad Ismail1, Abu Bakar Muhammad Raza1,2, Zarina Qasim3, Muhammad Zeeshan Majeed1*

... the export of Pakistani citrus fruits to overseas markets. Integrated pest management (IPM) is an appropriate and sustainable strategy for controlling fruit flies as it is based on integrated application of all available pest control methods with minimum implication of hazardous synthetic insecticides. In this study, five treatments including field sanitation (T1), pheromone-based attract and kill strategy (T2), protein-based bait method (T3), combination of ...

Abid Hussain1*, Hassnain Shah1, Muhammad Nadeem Iqbal2 and Tariq Sultan3

...se of micronutrients for citrus fruits and their application for off-season vegetables, use of Biozote for wheat crop, and Pak Seeder technology for sowing wheat crop by zero-till method. Keeping this in view, economic viabilities of these technologies have been determined through marginal analysis. Unfortunately, Pak-seeder technology could not be demonstrated effectively due to few institutional and technical limitations. Thus, technical issues in its manufa...

Sarwat Yousuf1, Shaista Emad2 , Mohammad Misbah ur Rehman3, Zehra Batool4, Sara Qadeer5, Yousra Sarfaraz1, Sheeza Sheikh1, Sana Sadaf6 and Tahira Perveen1,*

Asifa Hameed1*, Haider Karar2, Abdul Ghaffar1, Abid Hameed Khan1, Muhammad Mubashir1 and Ghulam Mustafa1

...t crop in Pakistan after citrus. Pakistani mangoes are famous globally for their unique sweet taste and are exported to the Middle East, China, Afghanistan, and different European countries. Although mangoes have been cultivated in Punjab, Pakistan since ancient times, yield of mangoes is still very low, about 8-10 tons per hectare. Successful pollination is required to achieve a bumper crop. In this regard, an experiment was conducted at Mango Research Instit...

Salman Ahmad1*, Muhammad Asim1, Muhammad Zeeshan Majeed2, Muhammad Atiq3, Yasir Ali4, Hafiz Bashir Ahmad5, Malik Abdul Rehman6 and Naeem Akhtar7

...B) is the major cause of citrus decline. The disease is currently prevailing in all districts of Punjab and posing severe threat to citrus production and export. The current research was designed with two objectives; (1) to study the population dynamics of Diaphorina citri, the vector of CG, and (2) to record the incidence of CG in different citrus growing areas of Punjab and estimation of...

Akbar Hayat1*, Muhammad Asim1*, Tehseen Ashraf2, Ehsan-Ul-Haque1, Rabia Zulifqar2, Maryam Nasir3, Ahmed Raza1, Fahim Khadija1, Sohaib Afzal1 and Shafqat Ali1

...arameters of rough lemon citrus rootstock. The growing substances comprising Soil + silt + sand (2:1:1), and Rice husk + sand + soil (2:1:1), shows the prominently significant impact on rough lemon, in contrast to others media. In this experiment, an eight-month-old seedling of uniform height were used. The container was a 14′′x8′′ size plastic bag.

...

Muhammad Noman Khan* and Ghulam Nabi

...e in growth and yield of citrus crops. Therefore, for the possible solution the current study Potassium fertilizer source and timing regulate growth, flowering and yield in trees of sweet lime (Citrus limetta L.) was conducted during the year 2019. Experiment was laid out in RCBD split plot arrangement with 2 factors and 3 replications. Potassium sources (Potassium chloride (KCl), Potassium sulfate (K2SO4) and Potassium nitr...
FARZANA SIDDIQUE*1, NAZIMA FIRDOSE2, MUHAMMAD ARSHAD2, WARDAH HASSAN2
& SHAFAAT YAR KHAN2
...
milk samples from citrus plantation zone in district Sargodha were
collected and analyzed for the presence of deltamethrin and malathion
pesticides using HPLC technique. Results showed that 100 % of milk
samples were contaminated with pesticide residues. Maximum
concentration of malathion (0.72±0.02 μg/kg) and deltamethrin (0.75±0.04
μg/kg) was found in cow milk samples coll...

Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1 

...r. Therefore, the use of citrus fruit juice as medicine to treat infections is supported scientifically by these findings.
 

...

Muhammad Raza Salik1, Muhammad Babar Shahzad Afzal1,2*, Ayesha Komal3, Muhammad Nawaz Khan1, Muhammad Ihsan Ullah4, Faheem Altaf5, Akbar Hayat1 and Hira Tariq1

...lign: justify;">In genus citrus and family Rutaceae, Kinnow mandarin Citrus reticulata Blanco is widely grown edible species in the world including Pakistan. It is enriched with vitamin C and many other phytochemicals that are beneficial for health. In Pakistan, Citrus reticulata occupies the dominant position and it is 85% of total citrus production. Th...

Zakia Panhwar1, Arfan Ahmed Gilal1*, Lubna Bashir Rajput1, Jamal-U-Ddin Hajano2, Shafique Ahmed Memon3, Naimatullah Koondhar4, Muhammad Ibrahim Kubar1

Sofyl *, A.R.; Soliman , A.M.; Mousal, A.A. and El-Dougdoug , Kh.A.

...tic tree of the Egyptian citrus gummy bark agent (Accession no. FJ984562) revealed 100%, 99.7% and 97.8% a moderate degee of similarity to CVd-11b (USA), CCaVd (Spain) and CCaVd (Egypt) respectively. The minimum free energy of a secondary structure for HSVd-EG-RNA was determined using its primary sequence at 370C. The sequence appeared to fold into a rod-like structure at -122.1 kcal mol-I while CCaVd-EG at -120.5 kcal mol-I. lhe five domains of the rod-like s...
...o symptom development of citrus viroids. The geographical distribution of the gummy bark disease in some world countries depend on high temperatures for viroid-symptom expression. So, correlation between climatic factors and gummy bark disease through GIS is studied. We designed a satellite map for the gummy bark disease distribution all over the world using the previous registered results. Superimposed maps of BIOCLnvf annual Mintemperature, Max-Temperature a...

Nasr-Eldin1 , M. A.; El-Dougdoug2, K. A.; Othman2, B. Ahmedi , Sabah A, and Abdcl-Azizl , S. Il.

El-Dougdoug, Kh.A. t , S.A. Ghaza12 , A.A. Mousa2, H. Fahmyj and A.R. sofy2 
...nd TN were obtained from citrus cvs. Grapefruit, Balady and Navel, respectively. These isolates Were differed in some of their external symptoms. The CPsV-EG isolates were detected by biological indexing, giving rise to oak leaf pattern (OLP) on Dweet tangor. The three isolates were differentiated using double antibody sandwich-enzymelinked immunosorbent assay (DAS-ELISA), woody indicator plants, differential hosts, peroxidase isozymes and activity, total RNA ...

Rizqan*, Hilda Susanty, Linda Suhartati, Azhar, Arief, Elly Roza 

...ch can be extracted from citrus fruit peels, waste materials, and other sources. The objective of this research was to prepare an eco-enzyme using citrus fruit peel (Citrus reticulata) and analyze its organoleptic properties as well as its inhibitory effects against Staphylococcus aureus and Escherichia coli pathogens. Eco-enzyme was prepared from citrus...

Abdul Haseeb1, Yasir Iftikhar1, Muhammad Ahmad Zeshan1*, Safdar Ali2, Rana Binyamin3, Salman Ghuffar4 and Muhammad Usman Ghani5,6

... quality and quantity of citrus fruit and make it prone for the invasion of other microbes. A survey was carried out for the assessment of nematode population distribution in all 7 Tehsils of district Sargodha (Pakistan) viz: Sargodha, Silanwali, Sahiwal, Shahpur, Bhalwal, Kot Momin and Bhera. Population densities of nematode were assessed in different genera of citrus crop i.e. kinnow, sweet orange, feutral’s early, g...

Wali Muhammad Mangrio1*, Hakim Ali Sahito1, Faheem Ahmed Jatoi1 and Fahmeeda Imdad Sahito2

SEHRISH MUSHTAQ1, MUHAMMAD SHAFIQ1, MUHAMMAD ASHFAQ*1 FAIZA KHAN1, SOHAIB AFZAAL1, UMMAD HUSSAIN1, MUBASSHIR HUSSAIN1, RASHID MUKHTAR BALAL2 & MUHAMMAD SALEEM HAIDER1

...f different varieties of citrus cultivated in the Sargodha region of Punjab Pakistan. The endophytic bacterial community associated with citrus was characterized and screened for antifungal activity against Alternaria solani which causes losses to crops. A total of twelve strains were identified based on morphological and biochemical tests following Bergey’s manual of systematic bacteriology. The antagonistic potential...

Biologia (Lahore)

December

Vol.65, Iss. 2

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