Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2
E-mail | Tomas.Jinnerot@sva.se
...ratory disease (CIRD) in dogs. Other disease agents include various respiratory viruses and co-infection can exacerbate the clinical signs. CIRD is highly contagious and can spread rapidly, especially among dogs in close quarters. Fast and accurate diagnostics enables the selection of proper management. Our objective was to develop a sensitive and specific real-time PCR for detection of B. bronchisept...
Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
...d tested against Meloidogyne hapla both in-vitro and in-vivo. All fungi proved to be efficient in reducing final population of northern root knot nematode M. hapla and giving vigour to the plants. In laboratory experiment, L. muscarium was the most effective against nematode eggs (95.6%) and second stage juveniles (J2) (95.8%) infection. In greenhouse experiment, similar trend was found. L. muscarium proved to be more ...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...root knot nematode, Meloidogyne incognita, and the nematophagous fungus, Lecanicillium muscarium, on tomato. The nematode population densities were measured by determining the number of galls and egg masses, juveniles and eggs per root system, and reproduction factor. Four initial populations (Pi) (500, 1000, 1500, and 2000 eggs) mixed with antagonistic fungal conidia levels (1×103, 1×104, 1×105, 1×106) were used during the experiments....
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...root knot nematode, Meloidogyne hapla, through soil application under greenhouse conditions. A significant (P=0.05) reduction in the number of galls and egg masses, eggs and juveniles (J2), and the reproduction factor (Rf=Pf/Pi) was observed in the plants treated with L. muscarium compared to the control. However, all chemical nematicides were also effective in reducing the nematode infestation significantly (P=0.05) in soil compared to the control, but there ...
Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3
...or cells when produced endogenously in the cells or applied exogenously. Ceramidases, which are main component of ceramide pathway catalyze metabolism of proapoptotic ceramide to anti-apoptotic sphingosine and other fatty acids. In this study we have evaluated the pro-apoptotic effect of ceranib-2, a human ceramidase inhibitor and of a short-chain C2 ceramide on human lung adenocarcinoma cells, A549 by determining cytotoxicity of these agents by trypan blue as...
Glomus mosseae (Gerd and Trappe) and Neemex Reduce Invasion and Development of Meloidogyne incognita
Atta ur Rehman Khan1,2*, Nazir Javed2, Shahbaz Talib Sahi2, Tariq Mukhtar1, Sajid Aleem Khan2 and Waqas Ashraf3
...d development of Meloidogyne incognita was tested in eggplant roots in greenhouse pot trials. Neemex (5 g, 10 g and 15 g) and G. mosseae (100 g, 150 g and 200 g) were applied as protective treatment. The roots of eggplant were inoculated with 1000 second stage juveniles of M. incognita. Eggplants inoculated with nematodes only served as control. Each treatment was replicated tenfold. Data were recorded after one week interval up to five we...
Uzma Farid Durrani1,*,Sagar Mal Goyal2, Asim Khalid Mahmood1,Cathleen Hanlon3, Susan Moore3, Muhammad Waqas1 and Raheela Akhtar4
... of rabies in humans and dogs living in Lahore, Pakistan.
...
Matiyar Rahaman Khan1,*, Sabyasachi Pal2, Ghule Tushar Manohar2, Somnath Bhattacharyya3, Amit Singh4, Prahlad Sarkar5 and Samuel Lalliansanga6
...t knot nematode (Meloidogyne incognita) infects passion fruit (Passiflora edulis) in Mizoram, India and infected plants showed declined symptoms and poor fruit yield. Infested plants displayed stunting and yellowing and thick-root symptoms. The association of the root knot nematode species was identified based on observations on morphology of different life stages, beta-esterase phenotype and amplification of rDNA and partial sequence homology of...
Urooj Fatima1, Arslan Mehboob2, Muhammad Abid3, Muti-ur-rehman Khan4 and Tahir Yaqub4*
...gnificant threats to the dog population, around the globe. To ascertain the current situation and genetics of CPVs in Pakistan, a total of fifty samples from sick dogs suspected for parvovirus infection were collected from different geographic areas of the country. Samples were screened for positivity and verified for CPV using PCR. From a 30% positive samples for hemagglutination, nineteen (38.4%) samples were positive in P...
Neenish Rana, Nosheen Ehsan, Awais Ihsan and Farrukh Jamil*
...ext-align: justify;">Pseudogenes were previously regarded as molecular fossils, non-functional by-products of genome evolution. However, it has been indicated by several lines of evidences that some pseudogenes are active. Using current data of NCBI we have retrieved 65 pseudogenes from the genome sequence of human pathogenic bacteria Helicobacter pylori (H. pylori) strain 26...
A. K. Chaubey and Satyandra Kumar
Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...root knot nematode, Meloidogyne incognita and wilt fungus, Fusarium oxysporum f. sp. lycopersici on tomato in laboratory and pot culture experiment. All the isolates were isolated by serial dilution and cultured in liquid protein supplemented broth medium and culture filtrates (CF) were prepared. In in-vitro test single eggmasses of uniform size were kept in 5ml CF in 5cm diameter Petri plates. Antifungal activity was tested by dual culture of wilt fungus with...
A. K. Ganguly and Uma Rao
Decades of researches in biochemical and molecular nematology at IARI
...nging to the genus Meloiodogyne. Further, molecular markers like RAPDs, AFLP, SSRs and PCR–RFLP of rDNA have been employed to study the molecular genetic diversity in major root knot and cyst nematode species of India to assist the breeding program for developing resistant varieties and other purposes like regulatory and nematode diagnostics. Attempts have also been to made to understand the molecular signaling events during compatible plant nematode int...
Jia-xiang Wang1,2,* and Peng Li1,2
...justify;">Ghrelin, the endogenous ligand for the growth hormone secretagogue receptor, has been identified in the pancreas of many vertebrates. While ghrelin has been found in the cerebellum and gastrointestinal tract of African ostrich chicks, little is known about its distribution in the pancreas ofthe African ostrich. In the present study, the distribution and morphological characteristics of ghrelin-immunopositive cells in the African ostrich pancreas were...
Adnan Yousaf1,Jia Wu1, Qaiser Shakeel2, Yasir Iftikhar3, Muhammad Irfan Ullah4, Uzma Tahira5,Mustansar Mubeen1 and Wubei Dong1,*
...ultivars against Meloidogyne incognita and Sclerotium rolfsii and their integrated management by using derosol, cadusafos and Trichoderma harzianum in the green house at 25 ± 4ºC. Primarily, 15 chilies cultivars viz., 28-2010, C-72, Sanam, C-73, Gola Peshawari, 27-2010, Tata Puri, C-302, C-33, C-19, 5-2010, C-68, 11-2010, 18-2010 and 15-2010 were evaluated against M. incognita and S. rolfsii. Two wee...
Muhammad Arslan Khan1,*, Sajid Aleem Khan1, Imran-ul-haq1 and Rashad Waseem Khan2
...Among nematodes, Meloidogyne incognita (Kofoid and White) chitwood showed the maximum frequency percentage (72.3%). Results confirmed the presence of fungi and nematodes interacting each other to enhance the disease. Paecilomyces lilacinus (Thom) and Trichoderma harzianum (Rifai) (alone and in combination) were used as bio-control agents against isolated pathogens at three different concentration viz., R, R/2 and R/4. Combination of bio ag...
LX Ye1,2, JX Wang1,2,*, P Li1,2 and XT Zhang1,2
.../div>...
Xiaoting Zhang1,2, Jiaxiang Wang1,2,*, Peng Li1,2,* and Lixun Ye1,2
.../div>...
Senol Celik1,* and Orhan Yilmaz2
.../div>...
S. Shahid Shaukat*, Mian Sayed**, Aly Khan, M. Azhar Khan* and Babar Qazi***
...ulus reniformis and Meloidogyne incognita had similar population density levels and were more or less equally suppressed by all the treatments in the order: Carbofuran > Tridax procumbens > Fertinemakil Plus > Withania somnifera. The yield of guar was only slightly increased but not significantly....
Aly Khan* and S. Shahid Shaukat**
...ominant species was Meloidogyne incognita. Species diversity (H’) was highest in Wadh while lowest in Ali Dasht. Equitability (J’) component of diversity was highest in Kork and lowest in Ali Dasht, while species richness (d’) component was highest in Alat. The soils associated with Pomegranate orchards were coarse-structured, alkaline with low maximum water holding capacity. In most cases similarities were low between localities....
Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2*
...ommon infection in young dogs and is caused chiefly by canine coronavirus (CCoV) and canine parvovirus (CPV). While, CPV is considered the most important cause of diarrhea, studies have shown an increasing prevalence and importance of CCoV around the world. Fatal CCoV infections have been described, however, there is limited information on the molecular diversity of CCoV in many parts of the world. In this study, canine fecal samples from diverse States of Bra...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
...bs were collected from 6 dogs with severe respiratory syndrome at the Animal Clinics of Nanjing Agricultural University in 2014. One viral strain was isolated from SPF embryonated chicken eggs. Sequencing analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed 93 to 99% similarity between each other. The CIV isolates shared high similarity (above 98%) to the H3N2 viruses from dogs in China. On the comparison of ge...
Muhammad Zameer Kayani1,*, Tariq Mukhtar2 and Muhammad Arshad Hussain3
...aging effects of Meloidogyne incognita have not been assessed on cucumber, therefore, the present studies report the effects of five initial population densities of M. incognita on growth and yield of cucumber and nematode reproduction. All inoculum densities caused significant reductions in growth and yield of cucumber and were found to be positively correlated with the latter. On the other hand, ages of plants at inoculation had negative correl...
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
...;
... 
Muhammad Iftikharul Husnain1, Aneel Salman1, Inayatullah Jan2*, Tahir Mahmood3
...trolling for potential endogeneity by using locality as an instrument. Unsurprisingly, the magnitude of temperature coefficient increases substantially when endogeneity is controlled and becomes significantly higher (6 percent to 13 percent) than the previously reported findings in the literature. The findings suggest that endogeneity is a crucial issue and must be controlled before carryi...
Shuai Shang1,2, Honghai Zhang2,*, Xiaoyang Wu2, Qinguo Wei2, Jun Chen1, Huanxin Zhang1, Huaming Zhong2 and Xuexi Tang1,*
...s2r genes in raccoon dog (Nyctereutes procyonoides) was still unknown. As a true omnivorous mammal, the Tas2r genes may play a critical role in food selection of raccoon dogs. Thus, we explored the evolution of Tas2r genes in raccoon dogs. In this study, Tas2r genes including eleven intact genes and four pseudogenes from r...
Ghulam Muhae-ud-Din1,4, Anam Moosa1, Umer Farooq Ghummen1, Muhammad Jabran1, Amjad Abbas1, Muhammad Naveed2, Abdul Jabbar1 and Muhammad Amjad Ali1,3,*
...ria. ...
Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1
...dal effects against Meloidogyne javanica. Anti-J2 antagonistic test divided the isolates into 5 antagonistic groups or AGs. AG1 was observed as the most antagonistic group, causing an average of 66.6% J2 mortality. Genetic variability among these isolates, was studied at molecular level by randomly amplified polymorphic DNA procedure. A selected set of three random primers was used for this purpose, which resulted in 113 bands on gel for the 15 isolates of T. ...
Tariq Mukhtar
...valuated against Meloidogyne incognita on tomato. The application of T. harzianum and T. viride significantly increased shoot weight and decreased root weight of tomato in a dose dependent manner. Doses of 8×103 and 1×104 cfu/g of soil showed maximum increase in shoot weight and decrease in root weight. On the other hand, both the antagonistic fungi caused significant reductions in number of galls, egg mas...
Muhammad Zameer Kayani1,*and Tariq Mukhtar2
...ive potential of Meloidogyne incognita on different cucumber cultivars commonly cultivated in Pakistan, therefore, the objective of the present study was to evaluate the reproductivity of M. incognita on fifteen cucumber cultivars with varying levels of resistance and susceptibility. Significant differences were found among all the cucumber cultivars regarding formation of galls, egg masses, fecundity and reproductive factor. Maximum galls and eg...
Tayfun Karataş
...ting substance levels, endogenous reserves in liver and muscle tissues of rainbow trout (Oncorhynchus mykiss). Eight-days of fasting caused a significant decrease in glucose, total protein, triglyceride, cholesterol, high-density lipoprotein and low-density lipoprotein levels as well as protein and lipid reserves in liver and muscle tissue of fish (p<0.05). The fasting period had no significant effect on the hepatic thiobarbituric acid reactive subst...
Matiyar Rahaman Khan1,*, Sabayasachi Pal2, Amit Singh2, Ashok Dhananjaybhai Patel3, Bhagubhai Ambaram Patel3, Tushar Manohar Ghule2 and Victor Phani1
...t knot nematode (Meloidogyne indica) on the roots of citrus and Bt-cotton was found in Gujarat of India. The detection of M. indica and its diagnostic symptoms on some hosts (citrus and Bt-cotton) was recorded and the nematode species was authentically identified as Meloidogyne indica Whitehead, 1968 which was first time described on citrus from Delhi, India. The species is further characterized based on...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...ly infested with Meloidogyne incognita in Layyah District. Six okra genotypes, including 19,224; 19,235; Pusa Swami; Ikra-1; Ikra-2; and Sabzperi China Red, were selected from our previous study according to their galling index and level of susceptibility. These genotypes were used as test genotypes for the interaction between M. incognita and the entomopathogenic fungus, Lecanicillium muscarium. The initial inoculum was measured be...
Tünay Kontaş Aşkar1,*, Şinasi Aşkar1, Olga Büyükleblebici2 and Murat Güzel3
...sis (CVL). A total of 30 dogs, comprising 10 clinically healthy and 20 dogs with leishmaniasis were enrolled in this study. The diagnosis of canine visceral leishmaniasis was performed by the immune fluorescence antibody test with antibody titter ≥1:128 and lymph node smear examination. In blood samples of dogs with visceral leishmaniasis, levels of malondialdehyde, total antioxidant ca...
Aisha Siddiqua1*, Munir Ahmad1 and Nusrat Habib2
...alysis and Multinomial Endogenous Switching (MES) regression model to analyze and compare the impact of different adaptation strategies in isolation or in combination on yield. The primary data of ‘Climate Change Impact Survey 2012-13’ used for this purpose. Crop-specific strategies of change in the time of sowing, use of inorganic fertilizer, change in irrigation, and varietal change. Change in irrigation practice gave rise to the highest yield su...
Khalid Khan1, Saima Liaqat2*, Somaiya Rasheed1 and Ihsanullah Kakar3
...s. However, orthodox and dogmatic methods, inadequate awareness and lack of animals’ hospitals lead to hamper livestock efficiency than the potential. Therefore, it is urged that government should make veterinary services available to the farmers at their door steps to improve their income, livelihood and standard of life. Moreover, this can be one of a feasible solution to the problems of poverty, malnourishment and starvation in backward and undevelope...
Muhammad Arshad Hussain1 and Tariq Mukhtar2,*
...-knot nematodes (Meloidogyne spp.). As very little information is available on the distribution of root-knot nematodes in okra plantations, therefore, in the present studies surveys were conducted to record incidence, prevalence, and severity of root-knot nematode species associated with okra in the major vegetable growing districts of the Punjab province of Pakistan. The overall incidence of 39%, prevalence of 85% and severity of 4.1 in terms of the ga...
Shamsudin Bojang1, Idris Abd Ghani2, Jugah Kadir1, Adamu Saidu Paiko1, Yasir Iftikhar3 and Muhammad Kamran3,4,*
...ia for nematode, Meloidogyne graminis infections on the turf grasses. Samples were collected based on the sparsely growth and chlorotic appearance of the greens. A total of 36 soils and roots sample were collected. Scanning electron microscopy (SEM) was used to identify the parasitic nematode. Both the field symptoms and SEM micrographs confirmed that the nematode isolate was M. graminis. Since this nematode has been known to damage the greens an...
Xuefeng Cao1, Guangneng Peng1, Xiaobin Gu1, Changliang He1, Guizhou Yue2, Jun Shi3,* and Zhijun Zhong1,*
Tariq Mukhtar1,* and Muhammad Arshad Hussain2
...nematode (Meloidogyne javanica)is becoming a serious threat to okra production in Pakistan. The damaging potential of this nematode has not been studied on resistant and susceptible okra cultivars, therefore, in the present study, the effects of six inoculum levels of M. javanica were compared on a highly susceptible cultivar of okra ‘Sharmeeli’ and a moderately resistant one ‘Sanam’. All the inoculum levels ...
Khansa Nazir1,*, Tariq Mukhtar1 and Humayun Javed2
...t-knot nematode (Meloidogyne incognita). The maximum mortality of juveniles was recorded at a concentration of 100 mg/ml followed by 75 mg/ml of AgNP. The minimum mortality was recorded with 25 mg/ml of AgNP. With the increase in concentration, there was a corresponding increase in the mortality of juveniles showing a direct relationship between mortality and concentration of nanoparticles. The effect of time on mortality was also found significant. Wit...
Muhammad Tariq Adnan Khan*, Tariq Mukhtar and Muhammad Saeed
...ine cultivars to Meloidogyne javanica. Brinjal Jamak was the only cultivar found to be moderately resistant. Two cultivars namely Brinjal Shilpa and Singh Nath 666 appeared moderately susceptible. Five cultivars viz. Round Black, Short Purple, Brinjal PPL, Global Brinjal PPL and Namyal Ratchburi behaved as susceptible. All the cultivars behaved differently regarding formation of galls, egg masses, number of eggs per egg mass and reproductive fact...
Obaidul Islam*, Shameema Khatun and Mustasim Famous
...f clinical conditions in dogs admitted at Central Veterinary Hospital (CVH), SAQ Teaching Veterinary Hospital (CVASU), Chittagong, and District Veterinary Hospital (DVH), Sylhet during the period from 22 September to 18 October 2018 at CVH, 20 October to 18 November 2018 at CVASU and 26 February to 21 March 2019 at DVH. The prevalence was analyzed on the basis of location, age, breed and sex. Among the clinical cases infectious diseases occupied the highest pr...
Manzoor Hussain*, Marie Maňasová, Miloslav Zouhar and Pavel Ryšánek
...-knot nematodes, Meloidogyne hapla, on carrots in a greenhouse. All fungi and chemicals proved to be efficient in reducing the infestation level of Meloidogyne hapla and providing better growth of carrots compared to their controls. Maximum reductions in nematode population were observed in the plants treated with Lecanicillium muscarium and both chemicals. Lecanicillium muscarium tr...
Ping Jiang1 , Zhihui Zhao1, 2, Xiaohui Li2, Mengyan Wang2, Lixin Xia2, Yang Cao3, Runjun Yang2 and Xibi Fang2*
...pression levels of the endogenous gene ABCA1 in bovine mammary epithelial cells (BMECs) to analyse the effect on intracellular triglyceride (TG) content by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, cell TG Assay and RT2 Profiler PCR array. The results showed that ABCA1 knockdown reduced the TG content in BMECs. On the other hand, the RT2 Profiler PCR array demonstrated that the expression levels of 12 gen...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
...rding brucellosis in pet dogs caused by B. canis is limited. In the present study, we conducted a comprehensive molecular, pathological, and immunohistochemical analysis to detect this pathogen in pet dogs. Molecular methods, combining three types of PCR assays, identified two strains isolated from two pet dogs as Brucella canis. Histopathological changes revealed extensive i...
Nadia Saeed1, Mian Sayed Khan2, Habib Ahmad3, Muzafar Shah4*
...hus species, Meloidogyne species and Trichodorus species. Carbofuran (inorganic) and poultry manure (organic) in recommended amounts were used for management and results showed that both were effective against phytonematodes. Value of P for the effect of Carbofuran in Mansehra and Abbottabad was 0.0001 and 0.0001 whereas for poultry manure it was 0.0001 and 0.0009 respectively.
...
Huanxin Zhang1,2, Hongshuo Tang3, Jun Chen1, Yu Zang1,2, Xuexi Tang1,2,* and Ying Wang1,2,*
...stics of African hunting dog TLR genes are scared. The available genome sequence of African hunting dog offers us the way to examine the innate immunity of this endangered carnivore. 10 TLR genes (TLR1-10) were initially identified from the African hunting dog and lesser panda. The results showed that most of the TLR genes were very conservative in the African hunting
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
...blished pig, cow, human, dog, sheep, mouse, and chicken, respectively. It showed that MC1R gene had a high conserved type among different species. The goat MC1R protein has a molecular mass of 34.65 ku, an isoelectric point of 8.70, which is weakly alkaline, and contains seven transmembrane domains typical of cell membrane receptor proteins. Sequencing analysis of the black, brown and white different color MC1R genes of Nubian goats revealed that there are thr...
Mubashra Salim1, Omeira Ibrahim1, Hugo Vilhena2,3,4, Carla Maia5, André Pereira5, Maria Shahzeen1, Shabana Kalsoom1, Asim K. Mahmood6 and Furhan Iqbal1*
...of hosts including cats, dogs, domestic ruminants, pigs, rodents and humans. The present study was designed for the molecular detection of Mycoplasma haemofelis and Candidatus Mycoplasma haemominutum in feline blood samples collected from various pet clinics in Pakistan, by Polymerase Chain Reaction (PCR), using 16S rDNA as the target sequence. Clinical and epidemiological data was collected in all animals included in the study. M. haemofelis<...
Sumaira Akram1, Sajid Aleem Khan1*, Nazir Javed1 and Saeed Ahmad2
Ali Asghar1*, Tariq Mukhtar1, Muhammad Usman Raja1 and Asim Gulzar2
...tudy, effects of Meloidogyne javanica and Ralstonia solanacearum were studied on chili singly and in combinations. Significant effects of M. javanica and R. solanacearum and their interaction were observed in case of shoot and root lengths and weights of chili. The mean lengths and weights of shoot and root varied significantly as a result of both the pathogens applied singly and in combinations. The reductions in these parameters w...
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2, Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1, Yuriy Kharkevych1 and Magdalena Gryzinska4*
...enchymal stem cells from dog adipose tissue during in vitro cultivation. Materials and Methods: Mesenchymal stem cells were obtained from dog adipose tissue by explant method. The cells were cultured in CO2 incubator by standard procedure. Expression of cytoplasmic and membrane proteins on dog stem cells from fat tissue at the IVth and Xth passages was exam...
Asim Faraz1*, Abdul Waheed1, Muhammad Mudasser Nazir2, Aneela Hameed3, Nasir Ali Tauqir4, Riaz Hussain Mirza1, Hafiz Muhammad Ishaq1, Rana Muhammad Bilal5
...xogenously or secreted endogenously, establishes in minutes the desired effects and metabolized to inactive products rapidly in the body. Irrespective of whether OT is ingested with milk or secreted into the milk, it is degraded readily by gut-enzymes so that it doesn’t reach the circulation in biologically-active form so there seems to be less or no harm in consumption of milk from OT-treated-animals. But OT has remarkable effects on milk quality, compo...
Tingting Li, Shuangshuang Geng, Long Xie, Huiyan Xu, Aolin Luo, Pengwei Zhao, Huan Yang, XingWei Liang, YangQing Lu, XiaoGan Yang* and KeHuan Lu*
...mals. However, whether endogenous GDNF is superior to xenogenic GDNF in SSC culture in vitro remains unclear. To answer this question, the buffalo GDNF gene was cloned and integrated into the PiggyBac eukaryotic expression vector, which was stably expressed in Sertoli cells, thus establishing a PB-GDNF-Sertoli cell line. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that the GDNF gene expression in the PB-GDNF-Sertoli cell li...
Mian Adnan Kakakhel1, Faryal Gohar1, Zahid Anwar2, Raza Ullah1, Muhammad Attaullah3, Shahbaz Ahmad4, Aamir Khan5, Kalimullah5*
...bsp;infection in pet dogs. To evaluate the main associated risk factors related with T. gondii exposure to this region. A total of a hundred number of blood samples (72 males and 28 females) were collected from Peshawar, Pakistan. The serum samples were analyzed for IgG and IgM by using enzyme-linked immunosorbent assay (ELISA), and statistical analysis carri...
Hasaan Aziz*, Fariha Altaaf, Attia Bashir, Abdul Basit, Syed Anam Masood Gardazi and Farah Haider
...modified live vaccine to dogs, occurrence of disease is not uncommon. Here, we sequenced and phylogenetically analysed the complete hemagglutinin gene of CDV from a male dog (14 months old) exhibiting clinically suggestive symptoms. With a characteristic amino acid residue for dog at Y549, sequence analysis showed geographically distinct pattern clustering the study strain to clade Asia-2 ...
K. Osei1† , A. I. Adama1 , E. C. Tagoe2 and J. Sackey-Asante1
Biochar effect on nematodes and insects population density, soil improvement and yield of okra
...oot-knot nematodes (Meloidogyne spp.) has been reported as the most serious, widespread and alarming which causes tremendous yield losses (Hussain et al., 2011; Kayani et al., 2013; Barros et al., 2014). Flea beetle (Podagrica spp.) is the most important insect pest of okra in Ghana and Nigeria (Obeng-Ofori & Sackey, 2003; AsareBediako et al., 2014a, Mobolade et al., 2014). It has been estimated that insect pests attack led to great reduction in okra yield...
Huma Abbas1*, Nazir Javed1,Muhammad Kamran2, Sajid Aleem Khan1, Hira Abbas3, Ehetisham-ul-Haq2, Abdul Jabbar1, Mehwish Naz1 and Ihsan Ullah4
...nt-size: small;">Meloidogyne incognita (Kofoid and White) Chitwood is the most widespread and destructive pathogen of tomato, difficult to control due to its wide host range. In present investigation effect of bio (Cure, Azadirachtin) and synthetic (Cartap, Virtako) chemicals was evaluated on the induction of systemic activity by using modified split root technique. Efficacy of different application methods; soil...
Moazam Ali1, Wajid Ali2, Ayhan Ceyhan2 and Zeeshan Ahmad Bhutta3*
Pigmentation Genome Influence in Animals and Human Interventions in its Course of Action
... cattle, horses, camels, dogs, cats, pigs) new variants of coat pigmentation are achieved by generating mutation in MC1R and ASIP allele. A row of scientists is working on genome sequences and mutations for getting a better and healthier pigmentation besides animal welfare. This review article contains a complete elaboration about the worth of pigmentation in animals, natural pigmentation process and new mutation and deviation in animal genome related to their...
Abida Parveen1, Sajid Aleem Khan1, Nazir Javed1, Anam Moosa1*, Khushboo Javaid1, Hina Safdar1 and Asma Safdar2
...nt-size: small;">Meloidogyne incognita (Kofoid and White) Chitwood (MI)is a potential threat to tomato (Solanum lycopersicum L.) production. In this study, the inhibitory effect of Moringa oleifera L. (MO) extracts and Steinernema kraussei Steiner (SK) as stand-alone treatments and in combination was assessed to suppress the reproduction and development of MI. Highly susceptible variety of tomato cv. ‘Money Maker’...
Ashraf Ismail Afia1 and Ahmed Soliman Mohmed El-Nuby2*
Soybean Genotypes Response to Root-Gall Nematode
...all nematode (RGN), Meloidogyne incognita race 2, under greenhouse conditions. Significant differences among the genotypes resistance degrees were achieved. Substantial variations existed among the soybean genetic resources with regard to the evaluated parameters. According to reduction percentage in reproduction factor (RPRF), the tested genotypes were ranked as resistant or susceptible to challenge nematode genus. The genotypes viz., G22, G111(100% RF) were ...
Muhammad Tariq-Khan1*, Syed Zanib Ali Gardazi1, Abu Daud Ahmad Khan1, Muhammad Ilyas2 and Ishaq Ahmad3,4
Virulence and Distribution Trends of Root-Knot Nematode (RKN) Fauna on Summer Vegetables in District Bagh, Azad Jammu and Kashmir (Pakistan)
...des belong to genus Meloidogyne with extensive host range. A comprehensive survey study was carried out to document host and non-host plants in cultivated fields of Bagh district, Azad Jammu and Kashmir. Total of 111 vegetable fields from 82 locations of study areas was surveyed during summer 2013, 2014, 2016 and 2017. Okra, Eggplant, tomato, cucumber, chilies, beans and cucurbits were found most frequently cultivated vegetables in the area. RKN was found on 8...
Wajahat Azeem1,*, Tariq Mukhtar1 and Tooba Hamid2
...des of the genus Meloidogyne badly affect crop production worldwide and cause huge yield losses. Among the known root-knot species, Meloidogyne incognita is by far the most destructive, widely distributed and the most dominant and prevalent. In the present study, the efficacy of a biological control agent, Trichoderma harzianum and an antagonistic plant, Azadirachta indica was tested against M....
Atef Mohamed El-Sagheer
Status of Phytonematodes in a Main Commercial Banana Production of Upper Egypt
...laimus, Longidorus, Meloidogyne, Paratylenchus, Pratylenchus, Radopholus, Rotylenchulus, Rotylenchus, Tylenchorhynchus, Tylenchus and Xiphinema. It was observed that there was variability in population density, frequency of occurrence and prominence value of some plant parasitic genera e.g., Meloidogyne in Abo-Teg and East Mangabad has the most prominence value (17.20 and 19.80) followed by Longidorus and Radopholus in Manfa...
Majid Shahi Bajestani1, Esmat Mahdikhani Moghadam2*, Reza Aghnoum3 and Hamid Rohani2
Study of Plant Parasitic Nematodes and Description of New Record (Rotylenchus alius) Associated with Barley (Hordium vulgare L.) in Khorasan Razavi Province, Northeast Iran
...itylenchus apus and Meloidogyne arenaria were reported as new host record from barley fields of Iran. Meloidogyne arenaria, was selected for detailed investigation at molecular level. Molecular traits on Meloidogyne arenaria species done by partial 18s ribosomal DNA primer and sequence results verified the morphometric studies and showed 99 percent resemblance to AB905316 sequence from Jap...
Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo
The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
...root-knot nematode, Meloidogyne incognita. The development of such strains by genetic manipulation by transferring gene (s) required for saponins production is effective in increasing crop yield together with fixing nitrogen. Escherchia coli is still used as a host for the recombinant DNA technology – based industrial production of proteins and peptides to manage root-knot nematode. Mi gene resistance in tomato plants has been utilized to manage M. incog...
Abdul Nabi Jatt1,2*
...zyme (exoglucanase and endoglucanase) activity was highly induced in addition to exogenous AHLs. While, both exoglucanase and endoglucanase activities were radically decreased, when the growth medium was supplemented with quorum-quenching (QQ) AiiA protein. The present study, for the first time provides evidence that the QS system via AHL molecules involved in regulating the production of two different forms of an extracellu...
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
...n function such as steroidogenesis, follicular growth, oocytes maturation, ovulation and corpus luteum formation. The aim of this study was to identify the polymorphisms in the exon 11 of bLHR/HhaI gene (SNP g.1553C>T) and its association with service per conception (S/C) of Pasundan cows of West Java, Indonesia. Total of 147 Pasundan cows were used in this study. The PCR-RFLP analysed three genotypes of CC (0.11), CT (0.57) and TT (0.32) were detect...
Natalija Grittner1, Radomir Mandić2,* and Milivoje Urošević3
...ys, ducks, guinea fowls, dogs and bees were analyzed. The number of populations these indigenous breeds in Serbia has been shown in this paper. It is proposed that 15 breeds (13 breeds and 2 strains), which are not protected by the Decree of the Ministry of Agriculture of Serbia on animal genetic resources, be included in the Decree in the future, in order to preserve and improve their numbers. An urgent establishment of the animal gene bank of Serbia has been...
Li Shengqing1,2,3, Zhang Xiyun2,3, Liu Shengcai2,3, Hu Guoyuan2, Fan Yuxia2,Liu Huaixin2, Wang Tingting2 and Zhang Yanming1*
... as yaks, Tibetan sheep, dogs, vultures, and birds, is also tested. Results show that the toxin is encoded by a type D botulinum neurotoxin gene. The DNA and amino acid sequences of the full neurotoxin gene comprise 3831 base pairs and 1276 amino acid residues, respectively. The nucleic acid and protein sequences of the toxin exhibits 99% homology with those of a known type D botulinum neurotoxin gene, and the composition of the functional domain of the toxin ...
Jen-Hong Chu1, Ya-Wen Lan2, Shyn-Shin Sheen3 and Angela Chien3*
Saima Maher1,3*, Shahina Fayyaz2, Nadra Naheed3 and Zarbakth Dar1
...al activity against Meloidogyne incognita (the root-knot nematode). The seven pure bioactive compounds have been isolated and examined, from which VA 4 and VA7 had a potential effect against M. incognita by showing 90% mortality at 0.1 g/mL after 48 hours of exposure. The LC50 0.0149, 0.1575 μg/ml of (VA4 and VA7), respectively found to be significantly effective than compounds VA5 (LC50 = 0.1575 μg/ml), VA 6 (LC50 =0.1575 μg/ml), VA2 (LC50 = 0.6269&m...
Khan Tamoor1*, Hafsa1 and Hanif Maryam2
...eta cateria against Meloidogyne incognita at banana orchards of District Lasbela, Balochistan, Pakistan. Crude extracts, essential oils and fixed oil of these selected plants were tested separately. The crude extracts of Z. multiflora and F. oopoda showed higher mortality as compared to A. santolina and N. cateria. The results showed a maximum mortality of 76% by Z. multiflora and F. oopoda at highest dose of 3 g/ liter which was followed by 72% of N. cateria ...
Md. Mukthar Mia1 and Mahamudul Hasan2*
... reservoir, like a stray dog, could be feasible. Distressingly, such a pathogen is currently endemic in Asian nations, including India, Bangladesh, Sri Lanka and China. Thus, the literature review encompassed comprehensive knowledge concerning contemporary disease occurrence with causes and transmission imperative for management practice. Besides, the study converged on afresh advanced treatment procedures, vaccine progressions, and public awareness efforts, w...
Houqiang Luo1*, Yanfang Lan2, Ping Gan3, Wenjun Zhou4, Meng Wang1,
Bing Hu5, Zhuning Zhang5, Yu Bai1* and Kun Li6*
...ic ehrlichiosis (CME) in dogs. We have investigated the prevalence of R. sanguineus and E. canis infection in pet dogs in Wenzhou, China. Serum samples were obtained from animal hospitals were examined for antibodies by commercial rapid in-clinic ELISA kits. Ticks were collected from dogs and molecular detection methods were used to identify the tick species and CME by amplif...
Arshad Khan1, Mohammad Ihsan1, Ali Hazrat1*, Mohammad Nisar1, Muhammad Laiq1, Maryam Bibi1, Nasir Ali2, Ulfat Naz1, Muhammad Zakria1, Nausheen Nazir3, Adam Khan4 and Muhammad Asif Nawaz5
... was 0.63%. A cluster dendogram was constructed for total seed storage proteins and divided 7 sub clusters, where CA1 and CA69 were found the most diverse genotypes. The main aim of this study was to explore morphological variation in order to generate data that could assist in determining the most appropriate tools that are adaptable to various environments....
Usman Asghar1*, Muhammad Faheem Malik1, Umer Rashid2, Naeem Mahmood Ashraf2, Sumera Afsheen1 and Muhammad Hashim1
...falo, goat, donkey and a dog. A set of degenerative primers was designed for Polymerase Chain Reaction (PCR) of the cytochrome b gene fragment. All PCR was accomplished at the same reaction conditions. Amplified products had a length of 400 bp, which was our estimated length. After the sequencing of these amplicons, In silico restriction analysis was performed for each specie. Restriction endonuclease TfiI was selected for further analysis. Restriction fragmen...
Salma Javed1* and Daniyal Siddiq2
...Root-knot nematode (Meloidogyne spp.) is a pest of tomato Solanum lycopersicum (L.), causing reduction in yield and quality. The use of natural plant products as biopesticides in the prevention and control of pests and diseases has been increasing. Nematicidal activity of “Black plum Syzygium cumini, leaf aqueous, ethanol and methanol extracts were investigated for managing the population of Meloidogyne javanica, in to...
Asma Hanif* and Shahnaz Dawar
... root-knot nematode Meloidogyne javanica using hatching and mortality test at different time intervals. Notable results were noticed by Kent-20 and Santonine-43 at all tested concentrations by reducing the hatching and complete mortality of M. javanica after 96 hours, while Cina (Q) showed greater reduction in hatching and mortality at 100% concentration followed by minimum hatching of eggs when used at 75 and 50% concentrations but in case of 200 and 30 (C) p...
Mohammed Abdel-Mageed Abdel-Aziz Abdel-Mageed1, Eman Alsayed Hammad2*, Nashaat Abdel-Aziz Mahmoud1 and Anas Farag El-Mesalamy1
...root-knot nematode, Meloidogyne javanica. All the tested materials affected the survival of the nematode juveniles and egg-masses hatched depending on materials property, concentrations and solvents used in extraction. The aqueous extract had the best percentage of mortality and inhibition of egg hatching compared to the acetonic and methanolic extracts. Whereas, aqueous extracts of deadly nightshade, winter cherry and chili pepper at concentration 500 ppm app...
Mahmoud M.A.Youssef and Wafaa M. A. El-Nagdi
...root-knot nematode, Meloidogyne incognita on roots of several plants were reduced gradually by increasing gamma irradiation doses. When tomato plants infected by root-knot nematode, Meloidogyne incognita were exposed to combined elevated atmospheric CO2 concentration (+100 ppm) and higher temperature (+2oC), tomato shoot dry weight and nematode control were increased compared to prevailing conditions....
Thomby Paul, Sreekanta Biswas, Sabiha Zarin Tasnim Bristi, Debashish Sarker, Saroj Kumar Yadav and Bhajan Chandra Das*
...act male German Shepherd dog, weighing 26 kg, with a poor body condition score (2/5) was presented at SAQ Teaching Veterinary Hospital, Chattogram Veterinary and Animal Sciences University, Bangladesh. The primary complaint of the owner was non-weight bearing lameness of the right forelimb of the dog due to accidental trauma. Physical examination revealed the dog was active and alert but n...
Mohamed S. Khalil
...root-knot nematode (Meloidogyne javanica) is one of the most destructive pests to vegetable crops worldwide. Non-fumigant and/or fumigant nematicides, as well as biological agents are the most utilized tools to manage the root-knot nematodes (RKNs). Environmental and health defects were notice with using synthetic nematicides. Thus, in this work an attempt was conducted to provide alternative approach could be listed in control measures of RKNs. Two pot experi...
Atef El-Sagheer1*, Anas El-Mesalamy1, Abdel-Monem Anany2 and Nashaat Mahmoud1
...e juveniles (J2) of Meloidogyne javanica were estimated in laboratory and greenhouse conditions. Results revealed that all the tested culture filtrates caused significant reduction in number of J2 in all treatments, compared to the non-inoculated control, where the hatching inhibition and mortality percentages increased with an increase in exposure time. At 24 hours, increased the mean number of J2 hatched slowly than in first stages of experiment. Similar pat...
Yang Yang1,2, Ji Shao1,2, Mingxu Zhou3,4, Qiangde Duan1,2, Xinyi Zhang1,2 and Guoqiang Zhu1,2*
...ed with the ability of endogenous synthesis of 3OC12-HSL and C6-HSL. At the same time, 200 μmol/L 3OC12-HSL and C6-HSL were added into the culture medium, respectively, and co-cultured with ETEC strain C83902. ETEC strains were stimulated by exogenous and endogenous long-chain and short-chain AHL molecules, respectively, and their biological characteristics, such as growth characteristics, biofilm formation ability, and a...
Amal H. Mostafa, Mohammed Y. Matoock, Heba M. A. Khalil*
...gement program for stray dogs. Eight adult native dogs were collected from Maadi district streets in Cairo. and brought to the clinic to assess their aggression level. They were exposed to a 10 behavioral test series, including friendly greeting, room behavior, leash manner, look test, sensitivity test, tag test, squeeze test, food behavior, toy behavior, and dog to <...
Jiao Ma*
...t injury. This role of endogenic cardiac stem cells can be regulated by factors. Numerous causes, such as paracrine and autocrine factors, extracellular matrixes and genetic factors. In general, it is well known that these variables could have an appropriate role to improve the effectiveness of the treatment of heart lesions. The work of cytokines and growth factors has been found to play an important role in increasing endog
Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*
...sterilized Chinese rural dog was found to have a lump in the groin area over the past two months. The dog visited the doctor due to inguinal mass, loss of appetite, dyspnea, fever, and other symptoms. Blood routine examination displayed declined MCV and lymphocytes, while augmentation of neutrophils. Serum biochemistry, SNAP cPL and blood gas analysis suggested that the dog have pancreatit...
Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*
...sterilized Chinese rural dog was found to have a lump in the groin area over the past two months. The dog visited the doctor due to inguinal mass, loss of appetite, dyspnea, fever, and other symptoms. Blood routine examination displayed declined MCV and lymphocytes, while augmentation of neutrophils. Serum biochemistry, SNAP cPL and blood gas analysis suggested that the dog have pancreatit...
Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2
...uman stool and fasces of dogs and cats. Two hundred stool samples from humans and eighty-eight fecal samples from dogs and cats were collected with gastric disorders. The presence of H. Pylori infection in stool and fecal samples were tested by ELISA and PCR methods. In ELISA the test utilizes H. pylori antibodies to selectively detect H. pylori Antigen in human stool and fasces of pet animals, and PCR by using 16srRNA prime...
Furqan Awan1,2, Sadia Nawaz3, Muhammad Muddassir Ali3,*, Asia Iqbal4, Raheela Akhtar5, Sehrish Firyal3, Haleema Sadia6, Arshad Javid4, Muhammad Rashid7, Muhammad Sulman Ali Taseer8 and Zhenling Zeng1
...real tumor (CTVT) in pet dogs; and identify the potential factors that are playing important role in disease spread. A questionnaire-based study was conducted to identify the conditions and circumstances of CTVT affected dogs that presented to veterinary practices over a 12-month period. The practices were convenience-sampled on the basis of voluntary participation. Forty practices were approached, and veterinarians from 24 ...
Noura El-Shahat Attia1*, Abd El-Khalek Ramadan El-Sheikh1, Mohamed Omia Siam2
... Zinc (Zn) deficiency in dogs. The clinical study was conducted on two groups of dogs. The first group consisting of seven dogs which was clinically healthy and used as a control group, and the second group consisting of sixteen dogs “German Shephard” at different ages which was suffering from decrease appetite, stunt growth rate, as well as ...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...amages the spermatozoa endogenously. These stresses can be controlled with the inclusion of antioxidants in semen extender at the time of cryopreservation. In the current study, semen from four (n=4) healthy Nili Ravi buffalo bulls was collected through artificial vagina and Gallic acid was added to the semen at 1 µM, 15 µM, 30 µM, 45 µM, 60 µM, and 100 µM. A total of six groups were prepared. One group was kept as control w...
Chandrakala Rana1*, Deepak Subedi1*, Shanti Kunwar1, Rajesh Neupane1, Birendra Shrestha2, Khan Sharun3, Dinesh Kumar Singh4 and Krishna Kaphle2
...fy;">A mildly dehydrated dog was presented at Veterinary Teaching Hospital, Paklihawa Campus, Tribhuvan University with symptoms of urinary inconsistency, haematuria, and inappetence. Radiography and ultrasonography revealed the presence of calculus in the urinary bladder. Cystolithotomy was performed and the dog responded to the intervention and recovered uneventfully....
Hazel Tamakan* and Huban Gocmen
...dintermedius in cats and dogs with pyoderma and otitis externa by conventional culture methods, automated identification system and PCR-RFLP. This is the first study to research the presence of MRSP in cats and dogs in Cyprus. The PCR-RFLP method based on the pta gene was confirmed to S. pseudintermedius. Methicillin resistance results were revealed with VITEK 2 and mecA gene detection was performed by PCR. A total of 100 sa...
Tarık Bugra Saruhan and Alptug Sarı*
...predator pressure, stray dogs, roads, agricultural drugs, and environmental pollution....
Mohamed Saeed M. Hassan¹, Hitham Abdel-Saeed1*, Kawkab Abd El Aziz Ahmed2, Ossama Mohamed Abdou1
...al alterations in anemic dogs that were admitted to the teaching veterinary hospital at faculty of veterinary medicine, Cairo University and private clinics in Cairo and Giza governorates, Egypt. A total number (71 dogs) was included in the present study. This number comprises 16 apparently healthy dogs and 55 anemic cases. Historical data, clinical and hematobiochemical investigations wer...
Heba El-Zahar*, Zeinab Abd El-Rahman, Abbas El-Naggar
...as prognostic markers in dogs with IBD. After a detailed clinical, laboratory and ultrasonographic examination 21 IBD dogs with symptoms of chronic gastrointestinal diseases were chosen for the study. In addition to 11 healthy dogs served as control group. In comparison to controls, hematological analysis revealed significant variations (p<0.05) in total leukocyte count, hemoglobin, and...
Ni Putu Vidia Tiara Timur1*, Maria Maliga Vernandes Sasadara2, Purwanta1, Anak Agung Jayawardhita3
...ar old female local Bali dog weighing 13 kg presented to Udayana University Veterinary Hospital with swelling in the mandibular region and was reported that the swelling has been observed and was getting bigger since the dog was 8-month old. An initial examination identified a large volume of fluid. In 3 month period, the aspiration was performed twice, collecting a 8 cc and 5 cc clear yellowish fluid for each aspiration. Th...
Amal Hamad1, Ashraf M. Abu-Seida2*, Faisal A. Torad2, Nahed S. Thabet3, Shabaan M. Gadallah1
...dol HCl and Meloxicam in dogs. Forty clinically healthy mongrel dogs were randomly divided into 4 groups (10 dogs each) according to the analgesic agent used. Doses of 0.5mg/kg Nalbuphine HCl, 5μg/kg Fentanyl citrate, 2mg/kg Tramadol HCl and 0.2mg/kg Meloxicam were given IV in groups A, B, C and D, respectively. The zero time for evaluation was 10 min for Nalbuphine HCl, 45 min for Tram...
Doaa Khairy1, Mohamed Ali Osman2 and Fatma Abdel Mohsen Mostafa1*
...leterious effect of Meloidogyne incognita as well as ameliorate tomato growth in vivo. A mixture of moringa or neem aqueous leaf extracts with different parts of canola viz. leaf, stem and root gave better results than did single ones. Dual application of neem leaf extracts and canola parts extracts exhibited detectable augmentation in plant biomass better than other treatments. However, triple application of canola, moringa and neem leaf extracts (41.0%) surp...
Hosny Kesba1, Ashraf Suloma2, Samy Sayed3*, Abdullah Abdel-Rahman1 and Shaimaa Diab1
...ticularly the genus Meloidogyne are a primary limiting factor in the production of many plants and is a major problem in organic systems. This study was carried out to examine the influence of irrigation with different effluent water sources, including semi-intensive tilapia pond (STP), intensive tilapia biofloc (ITB) systems, and well water (WW), on the reproduction of Meloidogyne incognita infecting eggplants7 or 45 days a...
A.J. Shoyombo1, O.O. Alabi, B.M. Falana1*, R.A. Animashahun1, S.O. Olawoye1, F.A. Okenniyi1, M.A. Popoola2, C.I. Ukim2, A.M. Ake, A.E. Jubril3
...ten occur but requires a dogged approach in treating affected animals. Atresia ani is a congenital embryological condition that often suffice when the hindgut refuses to connect with the perineum for the proper discharge of muconium. In this study, a three-day-old calf was observed to be suffering from Atresia ani at the Landmark University Teaching and Research farm. This condition was obvious due to the inability of the calf to pass out faeces since parturit...
Azra*, Muhammad Awais, Alam Khan and Muhammad Shoaib
...les do not suffer from Endogeneity problem....
Wael Felefel1*, Mohamed EL-Beskawy2, M. F. El -Dakroury3, Mohamed Morsi Elkamshishi4, Eman Sayed Mohammed5
...on on naturally infested dogs with hard ticks. Forty five stray dogs with age above 6 months were collected from Alexandria governorate with heavy or moderate ticks infestation. The captured dogs were divided into 3 groups, each group includes 15 dogs, and categorized as follows; group (1): (Positive control group), group (2): (Doramectin injected group)...
Judith Kiptoo1*, Daniel Mutisya2, Paul Ndegwa1, Ruth Amata3, Lucy Irungu4 and Rotich Godfrey5
...tified species were Meloidogyne incognita, Tylenchulus semipenetrans, Helicotylenchus dihystera and, Pratylenchus brachyurus. The most abundant species in all the surveyed localities was T. semipenetrans. Factor regression analysis results showed that modest rainfall amounts favoured high density populations of PPNs on citrus roots where soil types of Rackers in Baringo and Luvisols, Ferralsols and Cambisols in Machakos County were dominant. Tylenchulus, Meloi...
Eman Alsayed Hammad and Atef Mohamed El-Sagheer
..., it was found that Meloidogyne is the most common genus and harmful one. So, the efficacy comparative of Chitosan, Marjoram emulsion oil, Trichoderma asperellum, and Vermicompost as alternative eco-friendly control agents for root-knot nematode M. incognita infected Pomegranate were evaluated under greenhouse and open field conditions. Results indicated the ability of tested strategies to suppress M. incognita after being applied three times. Under protected ...
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
...laterally confirmed by endoglucanase assay. Enzyme activity measured using the glucose standard curve was 725 U/ml after acetone precipitation. Thermal stability and optimum temperature were found to be 60°C for maximum activity. Likewise, enzyme was found more stable at 6.0 pH. Various mono and divalent cations were studied against the cellulolytic activity exhibited significant enhancing and inhibitory effect upon different concentrations. Effect of inhi...
Muhammad Anwar Ul Haq1*, Tariq Mukhtar1, Muhammad Inam-ul-Haq1 and Azeem Khalid2
...oot-knot nematodes, Meloidogyne spp. are economically very important and cause losses to the tune of $ 125 billion per year throughout the world. The present studies were aimed to evaluate 21 eggplant genotypes against the most destructive nematode, Meloidogyne incognita, under greenhouse conditions. Of all the genotypes/varieties of eggplant, Janak and Pala were found resistant and moderately resistant to M. incognita respe...
Zaniar A. Abas1, Mohammed Omer Baba Sheikh2,5*, Hardi N. Aziz3, Omed I. Abid4
...rom suspected parvovirus dogs were taken from different provinces in north Iraq for PCR testing, and 76% of such samples were confirmed to be positive. Sequence analysis showed two CPV-2c, six new CPV-2b, and ten new CPV-2a variants. In the CPV-2 studied, several unique and existing mutations have been discovered, indicating the emergence of CPV-2c variation in Iraq. Genetic differentiation study based on the genetic variation on the VP2 gene has shown t...
Eman Alsayed Hammad1* and Mahmoud Mohamed Hassanin Hasanin2
...rol for suppressing Meloidogyne javanica and Fusarium oxysporum on coleus plants, Coleus forskohlii in vitro and greenhouse conditions. Nanoemulsions of thyme (droplets size was in the range of 25.4–32.9 nm) and spearmint (droplets size was in the range of 5.91–9.77 nm) at concentrations 4000 and 5000ppm separately, recorded the best results in vitro investigations, completely prevented F. oxysporum growth at all concentrations, and increased M. ja...
Chinar Mustafa Mohammed1*, Thamer M. Bashir1, Omar A.M. Al-Habib2, Farhad Ramadhan1, Mohammed Bassam1
...>Taurine, a ubiquitous endogenous sulfur-containing amino acid, possesses numerous pharmacological and physiological actions, including antioxidant activity, modulation of calcium homeostasis, and antiapoptotic effects. There is mounting evidence supporting the utility of taurine as a pharmacological agent against heart disease, including chronic heart failure. This study investigated the potential protective effects of taurine, on behavior and cardiovascular ...
Noha M El-Motaily1, Ossama M Abdou1, Heba S Farag1, Kawkab A Ahmed2, Mahmoud Saber1*
...usly reported in patient dogs with demodicosis due to the effect of stress caused by mites. This study was conducted on 38 dogs, 20 of them were used as control group and 18 were demodicosis infected dogs, for evaluation of hematological, biochemical and histopathological alterations. Beside detection of the antioxidant trace elements (Zn, Cu, Se) levels in dog
Asaad A. Fares1*, Mohamed M. Ghanem2, Yasein M. Abd El-Raof2, Hossam M. El-Attar2, Ameer A. Megahed2, Heba M. El-Khaiat2
...div>
Keywords | Acidogenic, Hypocalcemia, Lactation, Calcium, Health status, Dietary anion-cation
...
Neeranoot Detcharoenyos1, Nakrob Pattanapon1, Soontaree Petchdee2*
...ld male golden retriever dog presented to Kasetsart Veterinary Teaching Hospital, Kamphaeng Saen, with progressive paresis over two weeks. The dog was ultimately diagnosed with a spinal tumor by radiography and MRI. In this study, the dog was successfully treated by dorsal laminectomy of T8-T9 to excise an abnormal spinal mass, and a modified pedicle screw-rod was fixed to T7-T10 for stabi...
Oluwatoyin Adenike Fabiyi
...s in the control of Meloidogyne incognita pests of tomato was evaluated. Crude extracts of M. indica bark was fractionated on silica-gel (120-150 mesh) using glass column. The resulting fractions were investigated in screenhouse and field trials. The fractions were equated with carbofuran a synthetic nematicide. Each experimental pot containing 40 kg of pasteurized soil was inoculated with 1000 eggs of M. incognita in the screenhouse, while 2500 eggs was intro...
Saima Mehar1 and Salma Javed2*
...were tested against Meloidogyne incognitain vitro conditions. The second stage juveniles (J2) were exposed at 24, 48 and 72 hours in different concentrations of plant extracts. During in-vitro condition inhibition of egg hatchability and J2 juveniles’ mortality varied according to the concentration of plant extract. WC=1 showed 100% mortality after 48 hrs of exposure at 1% with the lethal concentration (LC50) values 0.168 mg/land all four concentration a...
Alsi Dara Paryuni1, Soedarmanto Indarjulianto2, Tri Untari3 and Sitarina Widyarini4*
...o in animals, especially dogs and cats. The close interaction of cats and humans reported to play an important role in dissemination of zoonotic diseases. Therefore, this study aims to diagnose and to treat of dermatophytosis in cats. Twenty cats with the age of 6-12 months were used in this study. Determination of dermatophyte infection in cats based on clinical signs and laboratory identification of fungi. Skin scrapes samples were cultured onto Sabouraud&rs...
Xinjun Zhang1,2, Pengyong Wang1,2, Huimin Ju1,2, Yanhong Wang1,2, Yang Yang1,2* and Guoqiang Zhu1,2*
...rains were isolated from dogs in Yangzhou, China. The adhesion-encoding genes (iha, fimH, papA, papC, papG allele I, papG allele I’, papG allele II, papG allele III, focA, focG, sfaS), virulence-associated genes (sat, cdtI, cnf1, hlyD), iron uptake system encoding genes (iroN, iut, ireA) were investigated, and the ability of biofilm formation was examined. In addition, UPEC in invading T24 cells, cytotoxicity, immune response and synthesis of proinflamma...
C.A. Angel-Sahagún1, J.E. Ortega Palomares1, A.A. Hernández-Rangel1, C. Cruz-Vázquez2, R. Montesinos-Matias3, M. Valencia-Posadas1 and A.M. Cruz-Avalos4*
...enocephalides canis, the dog flea, under laboratory conditions. Nine, monosporic cultures were isolated. Each were pathogenic when exposed by immersion at a concentration of 1x108 conidia/ml, causing between 10-100% mycosis at ten days post-inoculation. Four isolates identified as Bb9, Bb6, Ma9 and Ma10, were the most pathogenic as mycosis reached 100%, which places them as potential candidates to be used as biological control agents....
Eman M. Aboelela1, Mohamed A. Sobieh2, Eman M. Abouelhassan3, Doaa S. Farid4, Essam S. Soliman2*
... meet all the demands of dogs and cats. The study aimed to conduct a seasonal cross-sectional survey on the point prevalence (PP) of ectoparasitic infestations in dogs and cats concerning some host, agent, and environmental determinants. A cross-sectional study was designed to last for four successive seasons from September 21st, 2020 to September 20th, 2021. A total of 1393 cats and 1511 dog<...
