Kathryne E. Taylor1 and Karen L. Mossman1,2*

...tion for some of this confusion – namely, that ICP0 has important functions in the cytoplasm that have been generally disregarded, due to the fact that many frequently used experimental manipulations restrict this protein to the nucleus. This commentary discusses the significance of these findings to the ICP0 field.

...

Dallin D. Oaks

 

... sudden and immediate confusion of languages, or even a sequence in which a confusion of languages led to a scattering of the people. Indeed, a close examination of the account seems to allow an interpretation of events that is compatible with what linguists have observed about how languages can diversify, though some challenges may still remain in reconciling assumptions about the available post-Babel time frame versus the ...

Mohammed A. Rohaim1*, Rania F. El Naggar2, Ahmed M. Helal3, Hussein Ahmed Hussein1 and Neil LeBlanc4

...the cleavage site of the fusion (F) protein indicated the emergence of PPMV-1 in Egypt. Phylogenetic analysis of the F and haemagglutinin-neuraminidase (HN) genes indicated that the isolate clustered with the PPMV-1 strains recently reported from Israel within subgenotype VIb. Our findings report the emergence of PPMV-1 in Egyptian pigeons and the potential role these wild birds can play in the transmission and evolution dynamics of PPMV-1.

...

Muhammad Aslam

...systems, accelerating diffusion and adoption of latest agriculture technologies and inputs, enhancing good quality seed production, improving irrigation water management and improving reclamation and drainage.

...

Muhammad Mushtaq, Umer Sadique, Naila Chand, Iftikhar Ahmed, Said Sajjad Ali Shah, Ijaz Ahmad, Imran Ullah and Muqadar Shah

... effect of water based infusion of Withania somnifera was investigated in broiler chicks. One hundred and twenty (120) day-old broiler’s chicks were alienated into four treatment groups namely WST-0, WST-I, WST-II and WST-III with three replication. Group WST-0 was kept as a control, while WST-I, WST-II and WST-III were treated with W. somnifera water based infusion @ 10, 20 and 30ml/lit respectively. The means were co...

Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1

...d isolates. Agar well diffusion assay was used to monitor the antimicrobial activity of these strains both in mono culture and mixed culture. Streptomycin sulphate (10 μgml-1) and Amphotericin B (5 mgml-1) were used as positive controls. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts was also determined. Syzygium aromaticum was proven to have excellent antimicrobial activity against all tested microorga...

Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3

...ce (EtOAc) using disc diffusion method. However, the same concentration of crude (1 µg.mL-1) showed 62.20 ± 2.51% growth inhibitions against Fusarium oxysporum using agar well diffusion method. Further investigation, including various chromatographic and spectroscopic analyses on the separation and isolation of potent metabolites is suggested to get a useful lead structure for development of novel antibiotics to...
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...nd molecular analysis of fusion (F) and hemagglutinin (HN) genes of NDV strain isolated from an outbreak in a peacock flock was undertaken. Proteolytic cleavage site of F0 showed a cleavage motif (112RRQKR↓F117), representative of a velogenic serotype. Moreover, based on F and HN gene nucleotide sequence analysis, the isolate clustered as genotype VII closely associated to those reported from Indonesia. Further sequence an...
Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
...r antibiotics by disc diffusion method. Over all mastitis was found 52.5% (63/120) with leading subclinical (41.67%, 50/120) form of mastitis on overall milk collected data. Pathogen type was crowned by Staph. aureus with 74.5% (47/63) prevalence, following which were Streptococcus species (17.45%, 11/63), E. coli (3.17%, 2/63), and Bacillus cereus (4.76%, 3/63). Coagulase positive Staph. aureus (41 out of 47), and hemolysin ...
Mirza Imran Shahzad1,*, Anna Iqbal2, Farrah Ali1, Nuzhat Sial3, Muhammad Ashfaq4, Abul Hasanat5 and Azra Khanum6
...easured by using disc diffusion method. Supernatant fraction of E. coli was positive against Sal. typhi and supernatant fraction of P. multocida was positive against E. coli, which show that antimicrobial agents were secretory in nature. Sonicated fractions were on second order to produce antimicrobial activities. The freeze thaw method was least effective in secreting active antimicrobial agents from tested bac...

Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*

...spp. using brain heart infusion broth supplemented with horse serum. The blood was used for serum plate agglutination assay. Thereafter, the samples were subjected to genomic DNA extraction to detect Mycoplasma spp. using multiplex-PCR. Isolation and identification data showed that a total of 58/96 samples (60.41%) were positive for Mycoplasma infections whereas a total of 61/96 blood samples were also found positive using serum plate agglutination. However, t...

Anil Sehajpal, Saroj Arora and Parminder Kaur 

 

Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice
...was evaluated by disc diffusion method. Out of 44 plants tested, 36 plant extracts showed varied degree of antimicrobial effect at different concentrations against the pathogen whereas 8 plant extracts, viz. Abrus precatorious, Acacia auriculiformis, Bougainvillea glabra, Convolvulus arvensis, Hibiscus rosa-sinensis, Morus alba, Thevatia peruviana, and Withania somnifera did not exert any effect. Among all the plant extracts, A. sativum exhibited strong fungit...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...esis (SDS-PAGE). Disc diffusion method was applied for the evaluation of antibacterial activity of lactoferrin. Minimum inhibitory concentration (MIC) of lactoferrin was determined by 96-well plate method using ELISA reader. Results depicted that CM-Sephadex C-25 was a good cation exchanger for recovery of lactoferrin. Camel raw milk produced 2.4 mg/mL of lactoferrin. The lactoferrin isolated from camel milk exhibited promising antibacterial activity against <...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and  Kui Chen2,*
...erent between speculated fusion proteins. Under bioinformatics analysis, the T2R1 gene without introns was consistent with the structural domains characteristics of this family genes. The translation protein of T2R1 gene displayed with corresponding signal loci and functional domains of this gene family, and the result showed that the taste receptor translated by T2R1 gene belongs to G protein-coupled receptor super-family. T. fasciatus...
Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
...ence, phagosome-lysosome fusion, and apoptosis induction, as assessed in appropriate in vitro (cell culture) and animal models. Results showed the omp10 mutant was dramatically attenuated for survival in macrophages and mice than the parent strain 2308. With decrease in the spleen/body weight ratios of mice infected with omp10 mutants were noted, inhibition of phagosome-Lysosome fusion was dramaticly imp...

Jawad Anwar* and Zafar Iqbal 

...evaluated, using disc diffusion method against tomato wilt causing bacteria (Xanthomonas campestris and Clavibacter michiganensis). Extracts that resulted in strong activity were fractionated into acetonitrile and ethyl acetate followed by testing against human pathogenic bacterial strains (Eschericia coli, Pseudomonas aeruginosa and Staphylococcus aureus) using 96 well microtitre plate method. The Ethyl acetate fraction showed maximum zone of inhibition (24.2...
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
...erformed by agar well diffusion assay and microbroth dilution assay respectively. All isolates (n=5) were carbapenamase and MBL producers andNDM-1 gene carrier. These isolates displayed significant resistance against commonly used antibiotics including carbapenem and colistin proved to be most effective drug. Manuka honey manifested significant antibacterial activity against all test isolates with almost similar zone of inhibition (7.4mm) except E. cloacae<...

Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3* 

...GF molecules such as two fusion proteins, VEGF TrapR1R2 and VEGFR1(D1-D3)-Fc, and one DNA aptamer, RNV66, in an in vivo model of human A549 lung cancer. VEGF TrapR1R2 is a commercial anti-angiogenic fusion protein. Our earlier studies demonstrated the anti-angiogenic and anti-proliferative activities of VEGFR1(D1-D3)-Fc and RNV66 in in vitro functional assays. We compared the anti-tumorigenic properties of these three molecu...

Attaullah Ansari* and Nasreen Memon 

...lant species with the profusion of hoverflies whereas; temperature had a strong negative and significant correlation with the abundance of hoverflies. Moreover relative humidity, rainfall and cloudiness were also negatively correlated with hoverfly population but their correlation was not significant. Habitat wise variation in average population size, species richness, evenness and diversity index were also calculated. The highest and lowest degrees of populat...

Aneeza Jamshed1* and Syed Amir Gillani2 

...ringes 88.7%, blood transfusion 91%), signs (effects on liver 92.9%), symptoms (88%), prevention in high prevalent areas (sterilized syringes and screened blood transfusion 86.5%) and management (88.7%). This study showed that it may be an important school health based intervention to educate students about the transmission, prevention and management of hepatitis C, which may help in declining the disease burden. Hepatitis C...
Mahboob Ali1, Muhammad Avais1, Riaz Hussain2, Kashif Prince1,*, Muhammad Ijaz1, Mamoona Chaudhry 3, Sehrish Firyal4, Amjad Islam Aqib1, Naimat Ullah Khan5 , Mian Saeed Sarwar5, Hazrat Ali5, Shahid Hussain Farooqi1, Ikramul Haq1 and Muhammad Sajid Hasni3
...R isolates using disk diffusion method. MDR were tested in vitro for response against various antibiotics. A questionnaire was filled for estimation of association of assumed risk factors with prevalence. Nonparametric tests at 5% probability were applied to check significance of results. The study noted overall 33.98% (123/362) of MDR S. aureus from subclinical mastitis milk samples while 123 of 192 S. aureus isolates (64.06%) were confir...

Aslam Memon, A. M. Khushk* and Umer Farooq**

... slowness of varietal diffusion. Very high variations in varietal diffusion indicators were also observed across provinces, farm size and tenancy status categories.In conclusion, despite that major proportion of total sugarcane area was planted under recommended varieties, the varietal diffusion process among sugarcane growers all over the country was taking place on informal grounds and w...

 Amir Khatam*, Sher Muhammad, and Ijaz Ashraf**

ROLE OF INDIVIDUAL CONTACT METHODS IN DISSEMINATION OF AGRICULTURAL TECHNOLOGIES
...al contact methods in diffusion of agricultural technologies from 280 randomly selected farmer respondents. Researchers collected the data with the help of a pretested and validated interview schedule using survey technique. The results show that majority of the farmer respondents is aware of the farm visits (66.43%), demonstrations being conducted (62.50%) and home visits made by the extension staff (60.71%) as extension methods for dissemination of agricultu...
Dachuan Cheng1,2, Md Mahbubul Hassan3, Zhenhua Ma1, 2, 3,*, Qibin Yang1 and Jian G. Qin3
...ere: lordosis, vertebral fusion, neural spines bifurcation, connection of adjacent pterygiophores, haemal spine anomaly, neural spines anomaly, anomaly in pterygiophores and supernumerary neural spines. Results from this study add new knowledge to functional morphology of crimson snapper that would be useful to larval aquaculture of marine teleosts.
...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
...upon lifelong blood transfusion. Multiple transfusions expose them to many blood borne diseases, most commonly hepatitis B and C. The aim of current study was to determine the prevalence of HBV and HCV infections among thalassaemia major patients. The study was conducted from June – December 2016, at the Thalassaemia Centre, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad and the Pakistan Thalassaemia Centre,...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
... of IFNα2-Tα1fusion protein in E. coli using pET-SUMO vector and determination of its biological activity. SUMO-IFNα2-Tα1 was successfully expressed in soluble form with IPTG induction to final concentration 0.5 mM at 37oC for 4 h and purified using affinity chromatography. SUMO tag was removed from SUMO-IFNα2-Tα1 by SUMO protease and recombinant IFNα2-Tα1 was collected in flow through by affini...
Aqeela Ashraf1,2,*, Muhammad Imran1 and Yung-Fu Chang2
...was performed by disk diffusion method. Resistance and virulence genes were detected by PCR. The results showed that 100% of isolates were resistant to penicillin, 54% to ampicillin, 44% to tetracycline and 30% to streptomycin, while none of them was resistant to chloramphenicol. These E. coli isolates carried one or more antimicrobial resistance genes. Genes present with highest frequency were tetA (42%), tetB (28%) and ampC (26%)....
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
...ified Kirby Baur disc diffusion method. Zone sizes were interpreted according to CLSI 2016 guidelines. Next day every carbapenem-resistant isolate were further processed for MβL detection by EDTA method, zone size of Carbapenem disc only and Carbapenem disc impregnated with EDTA was compared ( >7 mm increase MβL positive, 0-5 mm increase MβL-negative).
Results: Out of total 12126 samples, 35.9% (n=4361) were culture positiv...
Munir Ahmad1, Aisha Hameed2*, Saba Khaliq3, Hamid Saeed Malik4 and Shahida Mohsin5
...ult in the production of fusion genes (BCR-ABL, TEL-AML1, and MLL-AF4). These chromosomal rearrangements have great prognostic and therapeutic significance. Amongst these, TEL-AML1 fusion gene represents a subgroup of ALL cases, which are associated with many clinically important parameters of good prognosis. The objective of this study was to detect the frequency of TEL-AML1 fusion...
Tanveer Hussain1,*, Masroor Ellahi Babar1, Marcos De Donato2, Abdul Wajid1, Asif Nadeem3, Zahoor Ahmad3, Waqas Ahmad Khan4, Sunday O. Peters5 and Ikhide G. Imumorin6
... of domestication and diffusion of modern day cattle. To better understand the origin and genetic diversity of native cattle breeds (Bos indicus) in Pakistan, partial sequencing of the mitochondrial gene cytochrome b (339 bp) was done in 136 individuals from 10 different breeds. Analysis of the Cyt b gene showed high conservation in Pakistani cattle as expected, with only 26 individuals showing nucleotide changes. Only 5 point mutations were present in ...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...ethods. The agar disc diffusion method was used to evaluate the antibiotic susceptibility. Virulence-associated genes were detected using PCR while association analysis was used to test the likelihood of strains carrying combinations of genes involved in toxin production and/or host immune evasion. Highest resistance was observed against beta-lactam group followed by cephalosporin, lincosamide, tetracycline, macrolides and aminoglycosides. No resistance was ob...
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...T) and combined disks diffusion (CDD) method, respectively. The MBL producing clinical isolates were further subjected to PCR for the existence of blaIMP and blaVIM genes. The carbapenem resistant A. baumannii (n=32), P.aeruginosa (n=26), K. pneumoniae (n=19), E. coli (n=16), C. ferundi (n=04), P. vulgaris (n=02) and E. cloacae (n=01), were isolated from clinical samples of ho...
Wajid Ali1,*, Asma Karim1, Muhammad Irfan2, Hafiz Abdullah Shakir3*, Ghulam Mustafa4, Zeeshan Shafique1, Ijaz Anwar1
...ells and severe lamellar fusion. It was concluded that, deltamethrine is highly toxic for non-target aquatic organisms like silver carp (Hypophthalmicthyes molitrix).
...
Nimra Naseer, Adeela Fatima and Imran Sajid*
...k, agar plug and well diffusion method were performed. Seven Streptomyces strains including NWMD-6, NWMD-7, NWMD-10, NWMD-11, NWMD-12, NWMD-15 and NWMD-16 were found to have significant antimicrobial activity against MDR bacterial pathogens. Secondary metabolite profiling of each methanolic extract by TLC and HPLC-UV signified the presence of unique and diverse metabolic compounds. The study predicts that the seactinomycetes strains are a potential reso...

Ocheme Julius Okojokwu1*, Maryam Bisola Adebayo2, Bashiru Shafa Abubakar3, Ibrahim Abubakar Yusuf2 and Joseph Aje Anejo-Okopi1  

...n, history of blood transfusion and trimester of pregnancy were statistically associated with prevalence of IgG antibody (p ≤ 0.05). There was a significant association (χ2 = 12.571; p = 0.028) between age group and IgG antibody. The age group > 40 years old had the highest prevalence of IgG antibodies while the age group 21 – 25 years (p ≤ 0.05) had the least (39.3%). Similarly, there was significant association (χ2 = 39.602; p < 0.0...
Mehwish Aftab, Tariq Mahmud*, Muhammad Asim Raza Basra, Aqsa Gulzar, Sumaira Basharat and H.M. Junaid
...S. aureus by disc diffusion method and antifungal activity against Macrophomina phaseolina, Fusarium oxysporum. The metal (III) complexes are highly active under permissible limits.
...

Asma Sohail1*, Kashif Sarfraz Abbasi1, Maryum Arif2 and Fatima Najam1 

...n method and indirect diffusion method using Ca-alginate. Pumpkin seed oil macrospheres showed 91.9 % encapsulation efficiency and 63.0 % loading capacity by direct extrusion method. However, indirect or diffusion method showed 43.0 % and 24.7 %. These results showed that direct method of encapsulation was suitable for oils to achieve maximum encapsulation efficiency and loading capacity at higher concentration of calcium ch...
Saba Irshad*, Aruba Muhammad, Ammara Muazzam, Farah Sarfraz Anmol and Rehman Shahzad
...hich minimize their transfusion frequency to 30 days in comparison to 7-15 days in Xmn1 -/- patients. Hematological analysis of thalassemic patients revealed low Hb, WBCs and platelets counts in contrast to control. The reported polymorphism was meant to be lowering the frequency of blood transfusions and to some extent responsible for diminishing the disease burden among ‘Thalassemia Major’ patients. 
...

Nadia Khan1*, Abdul Waheed1, Farrukh Siyar Hamid1, Naveed Ahmed1, Zafar Iqbal2, Seemab Ali1, Madiha Bashir1 and Hina Gul3  

...d seed oil, Agar well diffusion assay was employed. Effectiveness of the oil against selected bacterial species was measured in terms of zone of inhibition in millimeters. Amoxicillin was used as a positive control. The results showed that Clavibacter sp. and E. coli exhibit resistant to the oil extract while Xanthomonas sp. displayed nil results. The present study thus reveals that either the used bacterial species are more resistant strains or the oil extrac...

Syed Atif Hasan Naqvi1*, Ummad-ud-Din Umar1, Ammarah Hasnain2, Ateeq ur Rehman1 and Rashida Perveen1 

... poison food and disk diffusion techniques while best effective seven were trialed in glass house and field experiments to determine their efficacy against Xanthomonas oryzae pv. oryzae. In vitro and in vivo studies under different conditions showed significant response of Mentha piperita, Azadirachta indica and Aloe vera either tested individually or in combination of two or more than two decoctions against the bacterium in question. When used individually, M...

 Nazish Mazhar Ali1, Saiqa Andleeb2, Bushra Mazhar1, Anum Imtiaz1, Shaukat Ali2*

Assessment of antibiogram and resistogram of pathogenic bacteria isolated from irrigated wheat field water
...ar disc and agar well diffusion methods. Optimum temperature for growth of all bacterial strains was 37oC and pH 7 except Salmonella which had pH 6. Among all the antibiotics Oxacillin was less potent as three bacterial strains showed resistance against it while others antibiotics were more potent. Minimum inhibitory concentration of heavy metals for all bacterial isolates was different. It was concluded that irrigated pathogenic bacteria are highly resistant ...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
... enhancing effect of the fusion type of CpG motifs on SS DNA vaccine and growth of ram lambs. In this study, we constructed the ptS/2SS-asd plasmid, pCpG plasmid and the CpG-SS fusion type recombinant plasmid named ptCS/2SS-asd. Twelve ram lambs were randomly divided into three groups, namely, the treatment (Group T1, ptCS/2SS-asd; Group T2, pCpG + ptS/2SS-asd) groups and the control (Group C, pVAX-asd). The vaccine (...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...uction by Double Disc Diffusion test (DDDT) and Modified Hodge’s test. In 152 specimens, different species identified in order of their frequency were; Klebsiella pneumoniae (59; 38.8%), Pseudomonas aeruginosa (46; 30.3%), Escherichia coli (36; 23.7%), Klebsiella oxytoca (4; 2.6%), Serratia marcescens (3; 2%), Enterobacter agglomerans (2; 1.3%) and Enterobacter cloacae (2; 1.3%). Out of 103 carbapenem-resi...

Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*  

...a saligna, using disc diffusion method. The tested bacterial strains include, B. subtilis, E. coli, S. aureus, P. fluorescens and fungal strains were Aspergillus. niger, A. flavus, and D. turcica. The maximum zones of inhibition, 23.00±0.56 mm and 25.00±0.50 mm was given by ethyl acetate fraction of leaves against P. fluorescens and A. niger respectively. The crude saponins isolated from leaves did not give significant results against the tested ...
Hande Küçükönder1, Pınar Çelebi Demirarslan1,*, Aykut Burgut2 and Mustafa Boğa3
...s combined with the data fusion technique support the proposed hybrid approach’s results. In this context, the proposed hybrid approach was applied to evaluate the monthly production performance of a commercial enterprise with Lohman Brown genus 8000 chickens. According to the findings of the analysis, it was seen that January, March, October, November and December are the months when production performance is high. When these months were ranked among th...
Umar Farooq Gohar1, Hamid Mukhtar1,*, Ali Nawaz1, Ikram-ul-Haq1 and Asad Mehmmood2
... was assessed by disc diffusion method. Cephalosporin production was estimated by using spectrophotometric technique.
...

Ayesha Khan*, Zubair Ahmad Khan, Urooba Pervaiz And Mehmood Iqbal 

...ciation exist between diffusion of improved practices with adoption constraints and best teaching method used, while significant association exist with frequently used contact method. It is recommended that necessary inputs on subsidised rates should be provided to extension agents to make extension services more farmers friendly 

...
Tahira Moeen Khan* and Amat ul Mateen
...observed by agar well diffusion method.
...
Sumreen Hayat1,6, Muhammad Hussnain Siddique2, Bilal Aslam1, Habibullah Nadeem2, Asma Ashraf3, Muhammad Saqalein1, Mohsin Khurshid4, Naveed Shahzad5 and Saima Muzammil1,*
...as determined by disc diffusion method and isolates were screened for ESBL production using double-disc synergy test (DDST). Molecular detection for the presence of bla-SHV and bla-TEM was carried out by polymerase chain reaction (PCR). Of 392 blood and urine samples, 120 were found positive for microbial growth. Antibiotic susceptibility testing of K. pneumoniae isolates illustrated high resistance to ceftazidime (95.6%). Phenotypically,5...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...famethoxazole by disk diffusion method. Double-disk synergy test (DDST) was also performed for detection of Extended Spectrum β-Lactamase (ESBL)-producing organisms. As a result, majority of poultry isolates was susceptible to most of the tested antibiotics and highest resistance rates were detected for ciprofloxacin (2.8%) followed by ertapenem, imipenem, meropenem with equal resistance rates of 1.4% with CLSI interpretative criteria. However, analyzing ...
Iram Gull*, Muhammad Shahbaz Aslam, Imran Tipu, Roohi Mushtaq and Muhammad Amin Athar
...never efficiency of gene fusion is improved by inserting GC rich sequences. In this study, reliable scar-less gene fusion with the insertion of sequence of choice at junction sites has been described by optimization of various parameters of overlap extension PCR. The fusion of IFNα2b gene to the gene of human latency associated protein with insertion of HCV NS3 protease cleavage site...
Sher Bahadar Khan1,2,*, Zou Geng1, Cheng Yu-ting1, Asad Sultan3, Mumtaz Ali4,5, Irshad Ahmad6 and Rui Zhou1
...according to the disc diffusion method and antibiotic resistant genes {tet(A), tet(B), tet(C), strA/strB, aadA, aac(3)IV), aadB, sul1, sul2 and sul3, blaCMY-2, blaTEM and blaSHV} using mPCR. Resistance for LIN was the highest in overall (96.3%, 77/80) isolates as well as from pig farms (100%, 20/20) and different markets (100%, 20/20, 85%, 17/20). Resistance for other antibiotics such as AMX, TET, AMP and SXT was found 82.5% (66/80), 63.7% (51/80), 58.7% (47/8...

Ali Mahmoud Zanaty, Naglaa Mohammed Hagag, Neveen Rabie, Mahmoud Saied, Karim Selim, Saad A. Mousa, Azhar Gaber Shalaby, Abdel-Sattar Arafa and Mohamed Khalifa Hassan 

...quencing for the partial fusion protein. Phylogenetic analysis revealed that 20 samples are genotyped as very virulent NDVclass II of genotype VIIb, 4 samples were of high identity (94%-100%) with NDV class II of genotype II (vaccine strain) and 1 sample was phylogenetically related to NDV class II of genotype I with 98% identity. Furthermore, the intracerebral pathogenicity index (ICPI) for selected 5 virulent viruses reveals velogenic features with high path...
Shafqat Husnain Khan1, Shah Jahan2, Irshad Ahmad3,4, Sadeeq ur Rahman5,* and Tayyab ur Rehman4,*
..., while combined disc diffusion test identified 28 isolates as MBL producers among them. Of all carbapenemase producers, 25 isolates (30.8%) of carbapenemase-producing E. coli were found to harbor bla-VIM gene, while 13 isolates (16.04%) were found to carry blaIMP gene. We report on high incidence rate of carbapenemase producers among carbapenem resistant E. coli isolates.
...
Nida Zia1, *, Ayesha Maqbool2, Muhammad Safdar3, Umm-I-Habiba1, Altaf Mehmood4, Muhammad Usman5, Zahid Iqbal6, Javed Iqbal6, Shahid Mehmood6, Amanullah Khan7 and Sajid Umar8
...hes to evaluate FAdVs diffusion and characterization to design effective control strategies. 
...
Xiaopeng Tang1,2, Rejun Fang3,4,*, Guochang Pan1,2 and Kangning Xiong1,2,*
...; 0.05) the phosphate diffusion across intestinal mucosa significantly. According to the results, the Ussing chamber system can use to evaluate phosphate diffusion across intestinal mucosa, and EGF may inhibit the absorption of phosphorus.
...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
...e was studied by disc diffusion method, and the antibacterial ring diameter of each antibacterial agent was measured to determine its antibacterial ability. The molecular mass and purity of this polypeptide was 18.970kDa and 90%. Amino acid sequence analyses indicated that the N-terminal amino-acid sequence of this monomeric peptide was AAQSLLACGAARLYKIIQQWPHYRR. The results showed that under the effect of various bacteriostatic agents, Enterobacter aerugin...
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
...<0.05). Intravenous infusion of Erythromycin was provided in the acute phase for seven to ten days, and followed by the use of sequential therapy of Azithromycin for about three to four weeks, the children’s condition were gradually from acute stage to recovery stage. CXCL8 and its mRNA levels in peripheral blood of the sick children were all significantly decreased comparing with those in the acute stage (P<0.05). Further analysis showed th...
Shou-Hong Zhang1*, Ying Zhang1, Kun-Ming Wen2, Wei Wu3 and Wen-Yan Li3
...tic fibrosis by using Perfusion-Weighted Imaging (PWI). We established a BDL rat model of hepatic fibrosis. HGF carrier was administered through tail-vein injections. The gene therapy efficacy was evaluated in vivo. Before and after treatment the rats were examined with MR-PWI. The perfusion parameters of PWl were compared with the fibrosis stage. The total fibrous tissue in the liver of rats treated with HGF was lowe...
Fizza Anwar1, Muddassar Zafar1*, Zahid Anwar1, Raja Tahir Mehmood2
...termined through disk diffusion assays and Tautomycetin was observed as best antimicrobial agent having inhibitory effect on serine/threonine phosphatases leading to hindrance in bacterial growth. In addition, phylogenetic tree was also constructed using 18s rRNA gene of A. niger and gene clusters of secondary metabolites were determined through bioinformatics tools. 
...
Muhammad Shoaib1,3*, Arif Ismiyev2,Khudaverdi Ganbarov3, Aygun Israyilova3 and Sajid Umar4
...nd fungi by agar well diffusion method. In second step, minimum inhibitory concentration of test compounds was determined against bacterial species by using resazurin microplate assay. All the tested compounds exhibited variable antimicrobial properties against various test cultures. All the compounds showed stronger antimicrobial activity against Gram-negative bacteria as compared to Gram-positive bacteria and fungi. Compound III was found to be the mo...

Saleha Ashfaq1, Manzoor Hussain1, Nazish Bibi2, Jan Alam1, Muhammad Junaid2 and Sabi-Ur-Rehman3*

...essed using agar well diffusion method against bacterial and fungal strains, while cytotoxic activity was studied in the methanolic extract using brine shrimp’s lethality assay. All the extracts showed significant biological activity against Gram positive, Gram negative bacteria and selected fungal strains. Acetone, chloroform and methanolic extracts showed maximum activity i-e 39mm, 44mm and 35mm followed by ethyl acetate 27mm and n-hexane 26mm against ...

Farheen Shafique1, Shaukat Ali2,*, Saiqa Andleeb1, Abdul Rauf1,3, Syed Ayaz Kazmi1,3, Sadia Idrees4, Faisal Farooq1,3, Faiq Nawaz Khan3,4, Muhammad Saad-ul-Hassan1,3, Raja Awais Mumtaz1,3, Saba Khalid1, Zaheem Ashraf1, Hafiz Muhammad Tahir2 and Fazal-ur-Rehman5

...nfections but blood transfusion in Beta thalassemic patients is working with two faces, one as remedy and the other is key risk factor in the spread of silent killers. Thalassemia patients registered in Combine Military Hospital (CMH) Rawalakot and Sheikh Khalifa Bin Zayed Al-Nahyan Hospital, Muzaffarabad Azad Jammu and Kashmir Pakistan were studied for the viral hepatitis B and C prevalence. A total of 303 (including 164 males and 139 females) individuals, ag...
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...termined via the disc diffusion method and was observed most frequently to nalidixic acid (81.8 %), tetracycline (72.7 %), ciprofloxacin (54.5 %), and erythromycin (54.5 %), while low resistance to ceftriaxone (18.2 %) was detected. Among the 11 Campylobacter isolates, 9 isolates were multidrug resistant (MDR). The tet (O) gene, which is responsible for tetracycline resistance, was detected in only 6 isolates. Phylogenetic analysis of the tet ...
Mingsan Miao*, Hui Zhao, Jiaojiao Jia, Xiaoyan Fang and Yanyan Miao
...e cerebral ischemia-reperfusion mice model and to assess the protection mechanism of cerebral ischemia-reperfusion injury. The repeated cerebral ischemia-reperfusion mice model was used where bilateral common carotid artery was targeted to block blood flow, reperfusion and then re-blocking the arteries. Brain tissues were examined 24 hours post-drug admi...
Samia Afzal*,Sadia Zahid, Iram Amin, Muhammad Shahid and Muhammad Idrees
...ead  towards the confusion that the outbreak is either of Dengue or Chikunguna virus. In conclusion, confirmatory molecular characterization of the viral genome remains controversial and further studies are needed in this respect.
...

Cornelius Arome Omatola*, Samson Daniel Iyeh, Shedrach James Abuh, Charles Kehinde Mofolorunsho, Martin-Luther Oseni Okolo and Phoebe Queen Akoh 

...and husbands, blood transfusions, genital ulcers and trimesters were significantly related to a higher predisposition to STIs. Conclusively, a high prevalence of STIs was observed among asymptomatic pregnant women in the study area. Our findings highlight a concern with economic consequences. Hence intervention programs including comprehensive screening of pregnant women with increased access to health care and treatment of confirmed cases at an affordable cos...

Joseph Anejo-Okopi1*, Ocheme Julius Okojokwu1 and Onyemocho Audu2 

... intercourse, blood transfusion and vertical transmission. Therefore, there is a need for government commitment for HCV elimination program through making the DAAs available and its affordability to general population to curb the catastrophic cost of managing the epidemic. However, the diagnosis of HCV infection is faced with several challenges, therefore there is a demand for development of easy to use and inexpensive molecular methods for accurate diagnosis....

Dilshad Ahmad1* and Muhammad Afzal2

An Empirical Analysis of Economic Efficiency and Farm Size of Cotton Farmers
...ity based crop inputs, infusion of advanced mechanisation and regulating market mechanism for consistency in prices of crop inputs and outputs. It is essential to increase managerial competencies among farmers through increasing literacy, sufficient access of extension services, familiarity and infusion of advanced mechanisation and adequate access of formal credit through implementing adequate policy measures by concerned a...

Tahseen Ullah* and Noor ul Amin

In Vitro Antibacterial Activity of Medicinal Plant Extracts
...nd E. coli using disc diffusion method to uncover antibacterial potential of the plants extracts. The antibacterial investigation was performed in three replicates using disc diffusion technique using Nutrient Agar Media for the growth of B. subtilis and E. coli bacteria. The ANOVA for inhibitions zones indicates the effect of plants, bacteria strains, and concentration on growth of bacteria in the bacteria growth culture me...

Joseph Anejo-Okopi1*, Dorcas Yilger Gotom1, Noble Allison Chiehiura1, Julius Ocheme Okojokwu1, David Ochola Amanyi2, John Otumala Egbere1, Joshua Adetunji1, Otobo Innocent Ujah3 and Onyemocho Audu4

The Seroprevalence of Zika Virus Infection among HIV Positive and HIV Negative Pregnant Women in Jos, Nigeria
...he history of blood transfusion among the HIV positive participants, p=0.013. No association with educational level, employment, marital and resident, gestational trimester, fever, yellow fever vaccination, rashes and parity was found for anti-ZIKV IgG positivity in the groups. Our results showed high seroprevalence among the pregnant women, an indication that the ZIKV antigen that triggered the antibodies response is in circulation, therefore, suggest the nee...
Sher Bahadar Khan1, Mumtaz Ali Khan2,*, Hameed Ullah Khan3, Sher Ali Khan4, Shah Fahad5, Faheem Ahmad Khan6, Irshad Ahmad7, Nighat Nawaz8, Sidra Bibi9 and Muhammad Muneeb10
...ntibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes. Overall prevalence of Campylobacter jejuni was 14% being higher in Peshawar division (21%) followed by Bannu division (16%), Malakand division (13%) and Hazara division (8%). Over all highest antibiotic resistance was found against AMX (93%) followed by LIN (88%), AMP (86%), TET (82%), SXT (75), CHL (68%), CLR (65%), STR (50%), GEN (44%), OFX ...

Kecheng Zhu1,2,3, Peiying He1, Baosuo Liu1,2,3, Huayang Guo1,2,3, Nan Zhang1,2,3, Liang Guo1,2,3, Shigui Jiang1,2,3 and Dianchang Zhang1,2,3,* 

...s essential for myoblast fusion. Myomaker is regulated by myoblast determination protein (MyoD), a muscle-specific basic helix-loop-helix (bHLH) transcription factor in higher vertebrates. However, the transcriptional regulatory mechanism of the myomaker gene has not been explored in marine fishes. In the present study, molecular cloning, bioinformatic analysis and transcriptional analysis of Acanthopagrus latus myomaker (Almyomaker) were ...

Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo

The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
...vanica. Also, protoplast fusion between Pseudomonas fluorescens and P. aeruginosa to manage M. incognita was utilized. Many genes expressed in nematode feeding cells or the regulatory regions that control these genes have been isolated. Transproteins toxic to different plant parasitic nematodes can be expressed into tissues and cells feed upon by nematodes. Transgenic products with a potential to interefere with nematode physiology such as digestive enzymes or...
Amjad Ali, Kafeel Ahmad*and Shaista Rahat
...d by Kirby Bauer disc diffusion method using nineteen different antibiotics and multiple antibiotic resistance (MAR) index was determined. Prevalence of extended spectrum β-lactamases was studied by double disc synergy test. The ESBL genes blaCTX-M, blaOXA-10, blaPER-1, blaSHV and blaTEM were analyzed by PCR amplification among the isolates. Susceptibility to antibiotics was: imipenem (85.02%), meropenem (82.88%), cefepime (76.47%), piperacillin-tazobacta...
Nosheen Basharat1, Usman Waheed2,3*, Muhammad Arshad1, Noor e Saba4, Iram Masood1, Akhlaaq Wazeer1, Ahmad Farooq1, Sadaf Moneeba1Abdul Rauf5 and Hasan Abbas Zaheer2,3

 

...nt of Pathology and Transfusion Medicine, Shaheed Zulfiqar Ali Medical University, Islamabad, from November 2016 to June 2017. Total 282 samples were selected after routine blood screening of Hepatitis B and C, of which 75 were HCV positive (asymptomatic), 75 were HBV positive (asymptomatic) and 132 were from healthy (without HCV and HBV) voluntary blood donors. Selected samples were tested for TTV presence by ELISA and PCR assays. TTV DNA was detected in 55.2...
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
...quencing for the partial fusion protein, The eight NDVs isolates of velogenic genotype VII and contain the unique cleavage site motif 112RRQKRF117 with high relation to very virulent NDV Chinese strain Chicken /China/SDWF07/2011 strain with nucleotide identity percentage (99.3% -100%). The main causative agent of recent ND outbreaks in vaccinated broiler flocks in Menofia governorate, Egypt was found to belong to very virulent genotype VII. This strain was gen...
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Sadia Roshan2 and Javed Iqbal Qazi3*
... well as well as disk diffusion methods with maximum growth inhibition zones i.e., 15 and 17 mm by Sphingomonas sp. AsCh-P3 and Bacillus aerius AsCh-A7 respectively. Six probiotic bacterial isolates of known enumeration (C.F.U.ml-1) showed growth antagonism for the fish pathogen with inocula comprising of less number of C.F.U.ml-1. Highest survival rates i-e 63.5 and 70.17% were expressed by Sphingomonas
Hesham Saeed1*, Manal Abdel-Fattah1, Ahmad Eldoksh1, Farid S. Ataya2 and Manal Shalaby3
...chia coli revealed a fusion protein with a weight of 22.5 kDa after induction of expression with IPTG for 5 h. The recombinant IFNα was expressed in the form of inclusion bodies that were separated and solubilized in vitro and the protein was refolded using SDS and KCl. The folded protein is then purified using on Ni-NTA Agarose affinity chromatography and the purity was judged by SDS-PAGE. Moreover, the effect of the recombinant IFNα of...

Wajid Habib1, Saira Rasul2* and Hafiza Sadaf Zahra3

...rices and explain the diffusion between both products. Results of the study concluded that the volatility effect and mean of both interest plus exchange rate affects the selected commodities overall. Further, the volatility of wheat price significantly affects the price series of the rice. On the other hand, poultry prices do not affect the mean prices of beef as well as other products. It has been noted that prices significantly impact the current prices of m...
Samina Tahir Kiani1, Abdul Rauf1, Syed Ayaz Kazmi1,*, Nuzhat Shafi1, Madiha Khalid2, Naeem Latif1, Samina Gul2, Saghir Yousaf3 and Muhammad Arshad4
...positive PCR. Blood transfusion (p=0.0001) was found significant risk factor regarding spread of hepatitis B and C in the studied population.

 

...

El-Habbaa, A.S.

...el
immune-diffusion test (AGIDT) and indirect-Immunofluorescence assay (IFA) and
molecular detection of viral DNA using PCR. Positive results were showed in 36 of
samples (28/30 of sheep samples and 8/10 of goat samples)upon isolation on ECE by the
3rd passage and 22 of samples (17/28 of sheep isolates and 5/8 of goat isolates) using
AGIDT and indirect-IFA. PCR detection showed positive results with 3...

A. A. Rezk1,3, K. A. Alhudaib2 and A. M. Soliman2,3

...ed as 6XHis-tagged CYSDV fusion protein in Escherichia coli cells BL21 strain. The purified protein was immunized by injecting to New Zealand white rabbit using 6 injections at weekly intervals intravenously and subcutaneous. The rabbit blood was collected by bleeding and the antiserum was obtained. IgG was purified and conjugated to alkaline phosphatase. The conjugated antiserum was evaluated by indirect enzyme-linked immunosorbent assay (ELISA). The conjugat...
Bingjie Zhou1, Hitesh Bhagavanbhai Mangukiya1, Siva Bharath Merugu1, Fakhar-un-Nisa Yunus1, Yuchen Fan1, Zhenghua Wu1,* and Dawei Li1,2,*
...orescent functional AGR2 fusion protein as a powerful visual tracking tool to trace the external function of AGR2. This bioactive and auto-fluorescent his-tag recombinant AGR2-DsRed (hAD) protein was constructed by linking human AGR2 C-terminus with the red fluorescent protein derived from Discosoma sp. (DsRed) througha flexible linker. It was expressed successfully in Escherichia coli (E. coli) with optimized strain and cultivation parame...
Muhammad Noman Koondhar1, Asghar Ali Kamboh1,*, Muhammad Ammar Khan2, Riaz Ahmed Leghari3 and Parkash Dewani4
...inated using the disk diffusion method. The results indicated that 21.34% beef samples exhibited the Salmonella contamination. The statistical analysis exhibited a significantly higher (p< 0.05) prevalence rate in minced beef (28%) and lymph nodes (24%) as compared to the muscles (12%). The highest antimicrobial resistance was recorded against the antibiotics of penicillin group (88.5 to 93.7...
Kanwal Haneef1,2, Rakhshinda Habib1,3, Nadia Naeem1,3 and Asmat Salim1,*
...he microenvironment, the fusion products may perform tissue-specific function. Stem cell factor (SCF) is one of the important cytokine for cardiomyocyte survival following cardiac injury. The main objective of the present study is to determine the fusion potential of SCF transfected MSCs and cardiomyocytes (CMs). Rat bone marrow MSCs were transfected with SCF through retroviral system and co-cultured with rat CMs. Co-culture...
Tian Tian, Lili Luo*, Haiyan Wang and Lei Wang
...ameter value of pelvic effusion was increased, the success rate of conservative treatment with methotrexate combined with mifepristone tablets showed a decreasing trend, p<0.05. The risk factors that affect the success rate of methotrexate combined with mifepristone tablets are β-human chorionic gonadotropin level and progesterone level. To conclude β- human chorionic gonadotropin and progesterone monitoring is a key step for treatment of ectopic ...

Hafiz Muhammad Tahir*, Iram Liaqat*, Junaid Nadeem, Hammad Aamir and Shaukat Ali

... application and disk diffusion method to investigate the antibacterial potential of Neoscona mukherji and N. theisi egg and web silk. For disk diffusion method, silk was first degummed to form degummed silk solution’ (DgS), then the degummed silk was dissolved to form the dissolved silk solution (DSS). The DgS and DSS solutions were tested against selected common pathogenic bacteria (Esherichia coli, Salmonella typhim...

Saadu Mustapha1,2, Norsida Man1*,  Jasmin Arif Shah1, Nitty Hirawaty Kamarulzaman1 and Ahmadu Abubakar Tafida3

... Theory of Innovation Diffusion, Rogers and Burde’s Theory of Social Change. The findings from the study have shown that GSM, radio, television, camera, internet, computer, CD/DVD player and VHS video are the most commonly used ICT components in various aspect of extension work. The multiple regression results reveal that gender, age, level of education and working experience has no significant contribution to ICT adoption while marital status, awareness...
Shoaib ur Rehman1, Jabbar Khan2*, Raaza Malja Khan3, Maimoona Azam4 and Zeeshan Mutahir5
...having at least one transfusion dependent child and sporadic patients from different areas of karak region. Using the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique, all samples were analyzed for the most common β-thalassemia mutations reported in Pakistani population. The most common mutations detected in karak region were frameshift codons (FSC) 8/9 (þG) (HBB: c.27_28insG), followed by IVS-I-5(G>C), FSC ...

Dilshad Ahmad1*, Muhammad Afzal2 and Asif Ali Abro3

...ties focus priority to infusion and easy access of credit in formulating easy credit procedures for specifically subsistence farmers which will help in increasing livestock production, reducing poverty and unemployment in the region.

...

Syeda Shazia Bokhari, Aisha Waheed Qurashi*, Roheela Yasmeen*, Fouzia Yasmeen, Nabeela Nayab, Uzma Rafi

...pplemented Brain Heart Infusion (BHI) agar medium showed positive results and EPS contents showed higher carbohydrate content as compared to Protein contents. It was found that the microbes associated with earthworm (L. terrestris) have significant potential of adherence and can be exploited as a bioinoculant formulation for improving soil fertility. 
 
Novelty Statement | The study is novel in its...

Hailemariam Assefa1*, Tesfahun Dagnaw2 

...em seed oil by r disk diffusion method. Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus pyogenes were used as tested bacterial species whereas Yeast, Mold and Dandruff were selected as tested fungi. The effect was seen high on Staphylococcus aureus, while the Klebsiella pneumoniae was less affected by any of the used extracts. Methanolic extract was more active than petroleum ether and ethyl acetate extract on all three tested ...
Longjun Guo*, Jiaqi Teng, Juan Wang and Yukun Chen
... relationship between diffusion tensor imaging (DTI) quantification and cerebrospinal fluid τ (Tau) protein expression in leukoaraiosis patients. Inpatients who attended the Department of Neurology of our hospital from January 2018 to December 2018 were selected as study subjects, with 58 patients with leukoarais as the experimental group, and 60 patients without leukoarais in the same period as the control group. ELISA was used to detect τ (Tau) prote...

Muhammad1, Saadat Mehmood1,2, Atif Muhammad1*, Zaib Ullah3

...cal population, the transfusion of blood from an infected person and unhygienic practices during surgery and medical treatment are the main causes of hepatitis. It is suggested to implement high care on the occasion of surgery, injection, and blood transfusion. Furthermore, awareness campaigns regarding blood transmission and health hazards of Hepatitis should be launched for the population. 
 
<...

Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

...ation revealed pleural effusion of the dog; B-ultrasound showed nodular lesions in the spleen and enlarged lymph nodes in the abdominal cavity. Subsequent cytological examination of smear made from lymph node FNA and peritoneal fluid revealed a large number of lymphoblasts. The above symptoms could be combined to make a preliminary diagnosis of lymphoma in dogs. To further determine the type of lymphoma, the H and E, and immunohistochemistry were done. The imm...

Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

...ation revealed pleural effusion of the dog; B-ultrasound showed nodular lesions in the spleen and enlarged lymph nodes in the abdominal cavity. Subsequent cytological examination of smear made from lymph node FNA and peritoneal fluid revealed a large number of lymphoblasts. The above symptoms could be combined to make a preliminary diagnosis of lymphoma in dogs. To further determine the type of lymphoma, the H and E, and immunohistochemistry were done. The imm...

Hong Liu and Rui Xu*

...ing fetal renal blood perfusion in pregnant women with gestational hypertension. One-hundred single-fetal pregnant women diagnosed with gestational hypertension in our hospital from June 2017 to December 2019 were enrolled in the study. They were divided into mild prehypertension group (50 cases) and severe prehypertension group (50 cases). Another 50 cases of normal single-fetal pregnant women were selected as the control group. Within three months, all singl...

Jie He* and Ren Yang

...hole liver ischemia-reperfusion injury was established according to Pringle’s method. Results showed that compared with propofol group and vitexin group, levels of serum ALT, AST and LDH in propofol + vitexin group were reduced (P < 0.05), apoptosis index was reduced (P < 0.05), Bcl-2 protein expression was increased (P < 0.05), Bax protein and caspase-3 protein expression was decreased (P < 0.05). In conclusion, propofol and vitexin could up...
Alshimaa A. Hassanien1*, Asmaa Osama Tolba2, Asmaa A. A. Hussein3, Walaa M. Elsherif4
...encing was done. Disk diffusion method was used for antimicrobial resistance detection, while the impact of casein and α-lactalbumin was evaluated by well diffusion method. Results indicated that 53 (35.5%) and 35 (38.04%) of meat products and food handlers were positive for S. aureus, respectively. High degree of genetic homology was described between meat and human isolates of S. aureus. These isolates possess high a...

Muhammad F Tajol Ariffin1, Chai M Hian1, Muhammad Z Sukiman1, Mohd F Ghazali1, Siti M Zainal Ariffin2* 

...either by intramammary infusion of 1 x 103 cfu/mL Staphylococcus aureus (S. aureus) or phosphate-buffered saline (control). The haptoglobin (Hp), serum amyloid A (SAA) and α1-acid glycoprotein (AGP) levels in serum and milk were measured at pre-and post-infection (6, 24, 48 and 72 h) using the ELISA method. The SCC was determined by the direct microscopic method. Results revealed a time-dependent change of Hp, SAA and AGP in the infected group compared t...

Samah Samir, Amal Awad*, Gamal Younis 

...ting gyrB1 gene. Disk diffusion was used to test their antimicrobial susceptibility, and PCR was used to screen E. tarda isolates for the presence of four virulence-related genes (cds1, edwI, qseC, pvsA). In addition, the ability of E.tarda to form biofilm was tested by tube test. E.tarda was detected in 15 fish (6%) and a total of 40 isolates were recovered from organ tissue samples and confirmed based on phenotypic and molecular characterization. The frequen...

Min Li1, Shiwu Deng2*, Yiqian Peng3 and Hong Li2

...myocardial ischemia-reperfusion injury (MIRI) through mitochondrial and ER oxidative stress pathways. Left ventricular ejection fraction (LVEF), left ventricular end-diastolic diameter (LVEDd) and left ventricular end-systolic diameter (LVESd) of SD rats were measured by echocardiography, and the mRNA expression level of peroxisome proliferator-activated receptor gamma co-activator 1-a (PGC1 a), superoxide dismutase (SOD2) and citrate synthase (Cs) were detect...

Zunping Ke1, Chao Li2, Qiang Liu3, Gang Bai4, Junfeng Wang5, Ming Zhou5 and Xiao Dong5,*

...To avoid systemic hypoperfusion on bypass through loss of volume to the pulmonary circulation and shunting of cardioplegic solution to the pulmonary circulation through the fistula, a little increase of cardioplegic solution was given and the surgery was performed successfully. VSD was closed by using autologous pericardium patch, whilst pulmonary valvotomy, pulmonary arterioplasty and right ventricular outflow tract (RVOT) augmentation were performed using a ...

Fan Xu1,2*, Cheng Wu3 and Su Zeng1

...ous drip. The finished infusion should be used up within 6 h after the dispensing.

...

Jun Zhang1*, Xiaocao Xu2 and Min Cao2

...ge, number of blood transfusions per year, HBs-Ag, HCV-Ab and Anti-HBs. The study included 630 thalassemia major patients who received regular blood transfusions at a hospital in Bengbu, China. We found that out of 630 patients of the study, there were three HBs-Ag positive, so the prevalence of HBV in this study is 0.5%. The prevalence of HCV-Ab positive was 7.1 and these individuals had a significantly older mean age than ...

Kafeel Ahmad*, Muhammad Siqaf, Gul-E-Rana Abid, Ramla Somayya and Umama Qasim

...es production by disc diffusion method using the antibiotics cefotaxime (30 µg), ceftazidime (30 µg), cefepime (30 µg), amoxicillin-clavulanic acid (30 µg), imipenem (10 µg) and meropenem (10 µg). Three extended spectrum beta lactamases genes (blaCTX-M, blaSHV and blaOXA-10) and one metallo beta-lactamase gene (blaNDM-1) were analysed in the isolates. Eight (16%) isolates were positive for extended spectrum beta-lactamases p...

Jiang Wu*

...ere evaluated by agar diffusion method. Sub-inhibitory concentration (SIC) value, minimum inhibitory concentration (MIC) value and minimum bactericidal concentration (MBC) value were measured. The virulence gene expression of the four foodborne pathogens was analyzed by quantitative real-time PCR (qRT-PCR). A microplate reader was used to monitor cell growth at 600 nm with 1 h interval. Environmental scanning electron microscope (ESEM) was used to analyze morp...

Sher Bahadar Khan1,*, Mumtaz Ali Khan2, Irshad Ahmad3, Faheem Ahmad Khan4, Hamayun Khan1 and Sher Ali Khan5

...ntibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes through PCR. Among them, the highest prevalence of Staph. aureus was found in Peshawar-Mardan division (30%), followed by Malakand (28.5%), Bannu-Dera Ismail khan division (25%) and Hazara division (16%). Over all the high resistance was found against Lin (96.25%) followed by AMX (82.5%), TET (63.75%), AMP (58.75%), SXT (50%), CHL (48.7%), CLR (36.25%...

Tao Ren1, Guiqiu Cao2, Xiao Han3, Feng Tan1, Qiaoli Chen4, Shicheng Yang5 and Haiyan Zhang6*

Chinar Mustafa Mohammed1*, Thamer M. Bashir1, Omar A.M. Al-Habib2, Farhad Ramadhan1, Mohammed Bassam1
...re. The results showed infusion of Taurine 1mg/kg/hr, 2.5mg/kg/hr and 10mg/kg/hr non significantly change heart rate (BPM), QRS intervals, S amplitude (mV), T amplitude (mV), ST height (mV), JT height (mV), QT intervals (s) and QTc (s).
 
Keywords | ECG, Taurine, Isolation, Heart rate, QRS
...

Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby* 

...e to colistin in disc-diffusion assay, while in MIC (minimum inhibitory concentration) method 68.8% exhibited resistance. Colistin was recorded as 33.7% in chicken breast by HPLC. Furthermore, mcr-1gene was detected 54% using PCR. Sanger sequencing revealed the same identity (100%) between the three examined strains despite coming from different sources. It could be concluded that the practices inside poultry farms might be a possible source of spread of antim...

Muhammad Ajmal Khan1, Muhammad Yahya2*, Ali Hazrat2, Javed Khan3, Saeed Jan4, Tabinda Nowsheen2 and Inam Ullah5

...ins through agar well diffusion assay (small wells were made with cork borer and 6μl of plant extract was added to the wells). The antioxidant activity of methanolic leaves extract was evaluated through a well established protocol 2,2-diphenyl-picrylhydrazyl hydrate (DPPH) at various concentrations (50, 100, 150, 200, and 250 µL). The phytochemical analysis of methanolic leaves extract of T. camphoratum showed 2.80% alkaloids, 4.90% flavonoids and 1.8...

Hamdy Abdala Elnagar1, Wael Mohamed Wafa1*, Abdel-Khalek El-Sayed Abdel-Khalek2

... days) plus 1% NAC (IU infusion for 3 days) may improve the reproductive efficiency, health performance, antioxidant enzymes, and inflammatory reaction during the postpartum period.
 
Keywords | Antioxidant, Cows, Cytokines, N-acetyl-cysteine, Reproduction
...

Tariq Ali1,2*, Kamran1, Abdur Raziq1, Inam Ullah Wazir1, Anwar Ali1, Muhammad Ijaz Ali1, Muhammad Shuaib Khan2, Shakeeb Ullah2 and Sher Hayat Khan3

...ties by standard disc diffusion assay. The results showed that the prevalence of CM and SCM was 21.2% and 62.3% in cattle, while 17.8% and 68.9% in buffaloes, respectively. Additionally, it was noted that CM was higher in cattle, while SCM was higher in buffaloes. Amongst the bacterial isolates from mastitic milk the prevalence of Staphylococci spp. (24.6%) was at highest, which was followed by Escherichia coli (23.7%), then Streptococci spp. (8%) Salmonella s...

Anara Ryskeldina, Indira Iskakova, Nurgul Sarina , Alexander Shevtsov , Laura Syzdykova, Alexander Shustov, Yerlan Ramankulov, Marat Kuibagarov* 

... of boPAG1 failed. But a fusion protein made up of E. coli thioredoxin and boPAG1 was efficiently expressed in E. coli. Using the recombinant protein as an antigen, mAbs were generated. One mAb reacted with a protein present in extracts from cow placenta. The described results are useful for creating immunological reagents to detect pregnancy in cows, which is of great practical importance.

Keywords | Bovine pregnancy-associated glycoprotein 1,

Anum Syyam1,2, Nazish Saqlain1, Sidra Hareem1, Naghmana Mazhar1, Liaqat Ali3 and Samia Afzal2*

...t of Hematology and Transfusion Medicine in Children’s Hospital and the Institute of Child Health, Lahore, Pakistan over a period of four months from Oct, 2019 to Feb, 2020. Blood samples were collected from 350 healthy and voluntary group “O” donors (males = 320 and females = 30, median age 27 years ± 6.4 SD). Estimation of IgM type anti-A and anti-B titers was done using the standard tube technique and the samples positive for hemoly...

Nina Chen1, Shuang Xu2, Juanjuan Lin1* and Moslem Lari Najafi3

...tibiotics in piggyback infusion. In this study 5% glucose solution, fat emulsion and mixture containing 25 kinds of antibiotics were prepared by the proportion suitable for piggyback infusion (33:10:40). The number of fine particles was analyzed by automatic particle counter. According to results, even 24 h after preparation, 12 β-lactam antibiotics, clindamycin phosphate, teicoplanin, trimethoprim/ sulfamethoxazole and...

Sayed H. Al Habty1, Dina N.Ali2* 

... tried using the disc diffusion method; PCR was applied on (MDR) S. aureus isolates for discovering of blaz, mecA and VanA genes. 38% of yogurt samples had the highest prevalence of S.aureus followed by Damietta cheese (30%) and ice cream (14%). S.aureus isolates appeared high resistance to tetracycline, penicillin, oxacillin, ampicillin, streptomycin, amoxicillin/clavulanate and neomycin, in different percentages. blaz, mecA and VanA genes were detected at 60...

Shaolin Zeng, Shuai Yuan* and Kai Liu

...myocardial ischemic/reperfusion (I/R) injury in rats and its causative role. The differential expressions of miRNA in the rat myocardial tissue after post-ischemic treatment with sevoflurane were screened by miRNA expression profile chip. The potential targets of miR-26a-5p were predicted by application of Targetscan, microT-CDS, miRtargetLink and miRpathDB database. The luciferin reporter groups were used for verification. We detected the impact of miR-26a-5p...

Nauman Zaheer Ghumman, Muhammad Ijaz*, Arslan Ahmed, Muhammad Umar Javed, Iqra Muzammil and Ahmed Raza

...ere subjected to disc diffusion test, PCR, and SDS-PAGE analysis for the confirmation of methicillin resistance. The farm-associated and individual animal-associated risk factors were analyzed to check the association with S. aureus and MRSA-associated subclinical mastitis. The results revealed an overall molecular prevalence of 28.70% for S. aureus among which MRSA-associated mastitis was found 47.62% prevalent. The SDS-PAGE analysis depicted the presence of ...

Nuzhat Munawar1, Abrar Hussain1, Imran Sajid2, Zahra Noreen1, Muhammad Aslam3*, Zeeshan Rehman1 and Aamir Ali3,4

... evaluated using well diffusion and disc diffusion assay against various disease-causing microorganisms. In the case of well diffusion assay, the plant exhibited a maximum 16mm inhibition zone against B. subtilis a minimum 10 mm inhibition zone against P. aeruginosa, with acetone extract. The same extract seems inactive against K. pneumoniae as compared to control. When tested on S. aureus...

Salman Hussain1, Basit Zeshan2*, Rabiya Arshad1, Saba Kabir1 and Naveed Ahmed3

...ivity, cefoxitin disk diffusion test, and vancomycin minimum inhibitory concentration (MIC) E-test. The MRSA isolates were detected for the presence of mecA, mecC, and nuc genes. Congo red agar (CRA) method was used to assess the ability of isolates to form biofilms. The results of the study showed that the prevalence of MRSA was 48%. The MRSA isolates were highly resistant (100%) to penicillin, β lactamase inhibitors, cephalosporins, and macrolides. All ...

Dina N. Ali1*, Sayed H. Elhabtey2*, Manal M. Amin1  

...ns by using agar well diffusion method. The minimum inhibitory concentration (MIC) of MO and Nano-MO was 3% and 2%, on Listerea monocytogenes, Staphylococcus aureus, Salmonella typhi and E. coli bacteria, respectively. While, in inoculation of MO and Nano-MO in cheese complete reduction of examined bacteria was recorded at 3rd week of storage except for L. monocytogenes Nano-MO killed it at 2nd week. Overall acceptability (OAA) investigations showed better res...

Advances in Animal and Veterinary Sciences

1

Vol. 11, Iss. 1, Pages 1-188

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe