Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1

 

Life Sciences International Journal Issue 7 Volume 1
...d 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serot...

Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad

 

British Journal of Virology
... 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serot...

Souvik Ghosh, Nobumichi Kobayashi

British Journal of Virology
... Although studies on the genetic diversity of the antigenically important RVA VP7- and VP4- protein encoding genes are important for vaccine development or judging the efficacy of existing RVA vaccines, they do not always provide conclusive information on the overall and complex genetic makeup of RVAs, as the remaining 9 RVA gene segments are also susceptible to the forces governing RVA genetic

Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1

British Journal of Virology
...d 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serot...

El-Sayed M. Abdelwhab, Jutta Veits and Thomas C. Mettenleiter

Avian Influenza H5N1 in Egypt: What we Know and What we have to Know?
...ible to oseltamivir, but genetic markers indicating resistance to amantadine have increased dramatically. In this mini-review, we discuss recent findings on the H5N1 viruses endemic in poultry and humans in Egypt, and suggest possible options to control the infection.

...

Yanina Panzera1*, Nicolas Sarute1, Lucia Carrau1, Jaime Aldaz2, Ruben Perez1

...detailed analysis of the genetic diversity, evolution, and epidemiology of the virus in several countries. Our study allowed us to establish the presence of four genetic lineages in South America, being the continent with more lineages described to date, underscoring the high genetic diversity of the circulating strains.

...

Hanqin Shen1,3, Boliang Wu1,2, Guangwei Li1, Feng Chen1, Qingbin Luo1,3, Yimin Chen1, Qingmei Xie1,2,3*

...essure of vaccination by genetic evolution and antigen variation. Moreover, the ratio of L226 in the haemagglutin (HA) receptor binding site has increased, which indicates that the H9N2 virus has the increasing potential to infection and cause human epidemics. The threat to the poultry industry and public health should not be ignored. In this review, we summarised the findings of recent studies on H9N2 AIVs epidemics and strategies for their prevention in poul...

Xingdong Yang and Lijuan Yuan

...s and humans in terms of genetics, physiology, intestinal anatomy, and immune system, pig is particularly a suitable animal species for the modeling of human enteric pathogen infections, immune responses and for vaccine evaluations. These are the basis for the successful establishment of the infection and disease models for human rotavirus, human norovirus and human enterovirus 71 using neonatal gnotobiotic pigs. The differences between germ-free and normal an...

Weili Kong1, Guangpeng Ma2 and Jinhua Liu1*

...NS1 protein in different genetic backgrounds of influenza experimentations. Conclusively, it can be stated that CTT confers a positive role during the infection of influenza A viruses. However, this regulation is contributed by multiple factors and has variable impact on the pathobiology of influenza A viruses.

...

Gerald Misinzo1,*, Tebogo Kgotlele1, Epaphras A. Muse1, Jan Van Doorsselaere2, Mikael Berg3, and Muhammad Munir4

...eotide sequence and phylogenetic analysis clus- tered the PPRV from Tandahimba into lineages II and IV. The results give evidence of at least two separate introductions of PPRV into Southern Tanzania, underlining the transboundary nature of the disease, particularly in regions with uncontrolled livestock movements.

...

Nayle Maria Oliveira da Silva1*, Ricardo do Carmo Zanella2

 

Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*

 

...further pathological and genetic researches in Taiwan. Here, bioassay, multiplex RT-PCR and multiplex real-time RT-PCR were used to detect the current status of the two viroids showing HSVd was more prevalent than CEVd and simultaneous infections were common in field. Uneven distributions of both viroids also occurred in different tissues of citrus. Two multiplex molecular detection methods provide the understanding of the genetic

Galina N. Leonova1*, Ilya G. Kondratov2, Sergei I. Belikov2

 

...fying the biological and genetic characteristics of three Louping ill virus (LIV) strains in the south of the Russian Far East isolated from Ixodes persulcatus ticks and one strain was isolated from the blood of a person after a tick bite. Phylogenetic analysis showed that these strains are related to LI/A and LI/K strains isolated in England and Scotland.

 

...

Shahid Iqbal Awan1*, Syed Dilnawaz Ahmad2, Muhammad Amjad Ali3, Muhammad Shahzad Ahmed1, Aurangzeb Rao1

...er analysis, and greater genetic distance was detected among the members of cluster 6 and cluster 8 and cluster 7 and 8. Members of these two clusters can be utilised in transgressive breeding.

...

Temitope Oluwasegun Cephas Faleye1, Johnson Adekunle Adeniji1, 2 *

...ed and subjected to phylogenetic analysis. Both sites yielded two isolates each on MCF-7 cell line while on RD cell line one site yielded one and the other seven isolates. None of the twelve isolates replicated on L20B cell line. All were positive for both the panenterovirus 5l-UTR and partial VP1 PCR reactions while only the four isolates recovered on MCF-7 where positive for the EV-C-3Dpol/3’-UTR-PCR assay. The eleven isolates with exploitable VP1 sequ...

Ummara Waheed Khan, Raza Ahmed, Irum Shahzadi and Mohmmad Maroof Shah

Email | mmshah@ciit.net.pk

...e discovery of molecular genetics and biotechnological techniques, such as genome mapping, tissue culture, genetic transformation and in vitro regeneration offered new dimensions of research to improve important crop species including wheat. The tissue culture by itself has increased the crop improvement potential to develop new cultivars with desirable traits through somaclonal and gametoclonal selection...

Tauseef Taj Kiani1*, Mozammil Hussain1 and Hidayat ur Rahman2

 

...actical contributions of genetics to the agricultural world and has its most signi cant expression in maize than other cereal crops. Exploitation of this phenom- enon of superior performance of F1 progeny over their parents not only trans gured maize breeding schemes but also constituted the foundation of maize seed industry. Evaluation of per se performance of parental lines and estimation of heterosis and inbreeding depression are prerequisites to start hybr...

Mohammed A. Rohaim1*, Rania F. El Naggar2, Ahmed M. Helal3, Hussein Ahmed Hussein1 and Neil LeBlanc4

...the first pathotypic and genetic characterizations of the pigeon paramyxovirus type I (PPMV-1) from pigeon in Egypt during 2014. Pathotyping, based on the mean death time (86 hours) in eggs and the intracerebral pathogenicity index (1.2), and the amino acid motif, (112KRQKR116), at the cleavage site of the fusion (F) protein indicated the emergence of PPMV-1 in Egypt. Phylogenetic analysis of the F and haemagglutinin-neurami...

Mohammed M. Islam1, 3 *, Damian F. J. Purcell2 and George Kannourakis1,3

...ntify viruses of diverse genetic backgrounds. 

...

Tahir Khan, Raziuddin, Fakharuddin* and Muazam Jamal Razi

...as conducted to estimate genetic variability, heritability and genetic advance for important attributes in Brassica napus using 10 parental lines and their 21 F4 populations. Significant differences were observed among genotypes, parents, F4 populations and parents versus F4 populations for days to 50% flowering, days to maturity, plant height, primary branches plant-1 and m...

Ashley C. Banyard1* and Satya Parida2

...with both viral and host genetics being thought to influence the outcome of disease. Following the successful global eradication of rinderpest virus, peste des petits ruminants virus has also been targeted for global eradication by the OIE. Whilst the tools are available to attempt eradication there are a number of factors that will influence the strategy taken to progress with the aim of eradication. Here we describe current areas where knowledge ...

Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*

...es and peptides, reverse genetics, anti idiotypic antibody based vaccines and helper cell dependent diagnostics represent the present vaccine/diagnostic development strategies for the effective control and eradication of PPR. This review comprehend the current scenario of recombinant technology based vaccines and diagnostics, virus like particles (VLPs), reverse genetics approach etc., in the development of diagnostics and v...

Mohammad Mushfiqur Rahman1, Rokshana Parvin1, Ataur Rahman Bhuiyan1, Mohammad Giasuddin2, Shah Md. Ziqrul Haq Chowdhury3, Mohammad Rafiqul Islam1, Emdadul Haque Chowdhury1*

 

...were sequenced. The phylogenetic analysis revealed that PPRVs circulating in Bangladesh belonged to Lineage IV and they formed a separate sub-cluster along with recent isolates from Nepal, Bhutan and China. On genetic analyses, it appeared that the N gene of PPRV is less conserved as compared to the F gene. Two unique amino acid substitutions (K423Q and E426G) were observed in Bangladeshi and Chinese isolates in the partial ...

Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4

Muhammad Jurial Baloch1*, Ghulam Murtaza Channa2, Wajid Ali Jatoi2, Abdul Wahid Baloch1, Imdad Hussain Rind1, Muhammad Ahmed Arain1 and Ayaz Ali Keerio1

Christopher U Orji1*, Ignatius O Onyeocha2, Steven S Shaida3, Peter M Dede4, Bitrus Yakubu5, Elijah E Ella6 and Pam D Luka5

...n rely upon knowledge of genetic diversity between species’ population. Two species were studied: Glossina palpalis palpalis and Glossina morsitans submorsitans using two mitochondrial DNA fragment primers- Cytochrome oxidase subunit II (COII) and Cytochrome b (CytB). Twelve samples of laboratory-reared population of each species were used for the study. Sequencing data were used to calculate haplotype, haplotype diversity and nucleotide di...

Zuhao Huang1, Feiyun Tu2 and Dianhua Ke1*

...aphical distribution and genetic data suggest the elevation of M. v. americanus to a full species Rufous-crowned Bee-eater M. americanus, supporting the latest taxonomic arrangement by IUCN.

...

Iqra Mahmood1, Asif Nadeem1*, Masroor Ellahi Babar2, Muhammad Muddassir Ali1, Maryam Javed1, Aisha Siddiqa1, Tanveer Hussain2 and Muhammad Tariq Pervez2

...nd to identify potential genetic markers for resistance to mastitis. This article presents a systematic integration of complex biological interactions of 226 mammary gland genes to uncover underlying regulatory networks. Data were collected from various databases after having an exhaustive literature study. Network, functional and pathway analysis of the mammary gland genes were performed to find mastitis resistant genes, which helped us to identify prime gene...

Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3*

...don usage analyzed. Phylogenetic Bayesian and ML analyses were conducted, using a concatenated set of 12 protein-coding genes, and adding 16 other species of gobies (Gobiidae). The mitogenome sequences of O. lacepedii,

Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2 

...s study were to identify genetic polymorphisms of the huntingtin (HTT) gene and to further investigate its association with production performances in two Anhui local chicken breeds. A total of 800 chickens (400 Huangshan black chickens and 400 Huainan partridge chickens) of similar age and weight were evaluated in the present study. We extracted DNA from blood samples and analyzed polymorphisms of HTT gene as wells as possible association with egg production ...

Ali Raza Awan*, Sehrish Firyal, Muhammad Tayyab, Lala Rukh, M. Zia ul Haq, Shagufta Saeed and Muhammad Wasim

...ore. This study aimed to genetically identify and classify the indigenous wild Rose-ringed Parakeet of Pakistan using Cytochrome b (Cytb) gene polymorphism. Mitochondrial DNA of 24 unrelated Pakistani wild Ring-rose Parakeets was isolated and utilized for amplification and DNA sequencing of Cytb gene. The Phylogenetic analysis of the Cytb gene indicated that the Pakistani wild Rose ringed Parakeet was mono-phyletically clade...

Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3

...arkers in genotyping and genetic identification is of essential significance for preserving genetic diversity. The aim of this study was to reveal the genotype profile of indigenous types for TGC breed. In order to characterize TGC population, two polymorphic loci in cattle Calpain gene (CAPN1) and one polymorphic locus in Calpastatin gene CAST were studied. In order to sort the allele variants (G/C), PCR products, the ones ...

Imed Ben Salem*, Aymen Ben Ibrahim, M’barek Chetoui and Said Nouira

... study deals with morphs-genetic analysis of eight species viz. Lemniscomys barbarus, L. bellieri, L. griselda, L. limulus, L. macculus, L. rosalia, L. striatus and L. zebra; which have practically similar external morphologies although they spread in completely different geographical areas. The approach adopted was to identify landmarks on the skulls of all specimens using the tpsDig software, and then analyzing them through the program MorphoJ. Our results s...

Atta ur Rehman1, Sher Ali2 and Sajid Malik2*

... In order to observe the genetic differentiation in lower Khyber Pakhtunkhwa (KPK) region, ABO and Rh blood groups record of nine Pashtun populations, i.e., Bannu, Dera Ismail Khan, Hangu, Karak, Kohat, Kurram Agency, Lakki Marwat, South Waziristan Agency, and Tank, was collected. Absolute gene diversity, gene differentiation and genetic diversity were estimated to be 0.01, 0.019 and 0.515, respectively. There were the highe...

John G. Bruno

...erotypes if coupled with genetic identification techniques such as PCR or gene probe hybridization.

...

Fukuan Du1,2, Yan Li2, Zhixin Wen1, Ruguo Chen1 and Pao Xu2*

...very urgent to carry out genetic breeding to improve yield. In this study, we developed SSR markers by transcriptome, which could be used to genetic research. In this study, we performed transcriptome sequencing of six libraries from hepatopancreas samples in E. sinensis. After removing low-quality reads, short reads and reads belonging to mitochondria, a total of 278,260,960 clean reads corresponding to mRNAs were obtained,...

Bahattin Cak1, Orhan Yilmaz1, Siddik Keskin2, Tahir Bayril3 and Mohammad Masood Tariq4*

...considered in subsequent genetic evaluations. Furthermore, producing heavier carcasses (13-17 kg) in < 150 days may increase productivity and efficiency of the goat farming system.

...

Muhammad Saeed* and Iftikhar Hussain Khalil

...icated that non-additive genetic expression were pre-dominant for most of traits studied. Line BRF3 and testers ZM04 were good general combiners for most of traits under consideration, while F1 hybrids B4N11 × PS08, B4N11 × JZ, BRF1 × ZM04, BRF1 × KT10 were best specific combiners observed for several important traits including grain yield plant-1 which can be subsequently utilized in future wheat breeding to develop high yielding new w...

Sheraz Ahmad Khan and Ghulam Hassan

...imation of heritability, genetic advance and association of yield and yield related traits in wheat. For all the studied traits, mean squares showed the presence of significant variation (p≤0.01) among the genotypes. Heritability estimates were observed high (h2˃0.60) for all the traits. The highest heritability (0.93) was noticed in plant height followed by flag leaf area (0.89). The values of genetic advance were r...

Ibni Amin Khalil* and Raziuddin

... as well as non-additive genetic mechanisms were found important due to significant effects for both components i.e. General Combining Ability (GCA) and Specific Combining Ability (SCA) effects. Maximum GCA was shown by L-7 and T-1; whereas high SCA was noted for L-6 and L-7 with good ability to combine with T-3 and T-1 respectively, and could be utilized as genetic material in future breeding programs. Overall the biplot ap...
Waheed Anwar*, Muhammad S. Haider, Ahmad A. Shahid, Hamid Mushtaq, Usman Hameed, Muhammad Zia Ur Rehman and Muhammad Javed Iqbal
...rphologically as well as genetically by amplifying internal transcribed spacer region (ITS). Variability among Fusarium species based on ITS showed that it is not enough to score diversity within species and isolates. Additionally pathogenicity of isolated Fusarium species was evaluated against nymph and adult of Bemisia tabaci. Under controlled conditions different species of Fusarium restrained the growth of B. tabaci
Lingtong Ye1, Chao Cao1,2, Bin Tang1,2, Tuo Yao1, Ruixuan Wang1 and Jiangyong Wang1*
...). The intraspecific genetic distances of polydorids examined in the present study ranged from 0.33% to 1.67%, whereas the interspecific distances ranged from 18.88% to 24.79%. Such large barcoding gaps between intra- and inter-specific distances indicate that the COI is a suitable gene marker for molecular identification of polydorid species. Our results demonstrate that not only did all COI sequences from the larvae show greater than 99% sequence identit...
Guang-Xin E1, Yong-Ju Zhao1, Zhong-Yuan Zhang1, Yan-Guo Han1, Wei-Wei Ni1, Ming-Xing Chu2, Yue-Hui Ma2, Jian-Ning He3, Huai-Zhi Jiang4, Jia-Hua Zhang1, Zhong-Quan Zhao1, Yan Zeng1, Ri-Su Na1, Gao-Fu Wang5, Hang-Xing Ren5 and Yong-Fu Huang1*

Pam D Luka, Frank N Mwiine, Bitrus Yakubu, Joseph Erume, Ricardo Pérez-Sánchez, Hermann Unger and David Shamaki

...fluence the emergence of genetic variation and long-term persistence of the virus in an area. Since the first report of ASF in Nigeria, periodic outbreaks have continued to occur among domestic pigs with a few incidences in wild pigs. However, to investigate the risk of a tick-domestic pig transmission cycle in Nigeria, 3,288 serum samples were collected from domestic pigs from 10 states and analysed for anti-tick antibodies using the recombinant TSGP1 indirec...

 Ali Zurwan1,*, Muhammad Moaeen-ud-Din2, Ghulam Bilal2, Zia-ur-Rehman1,2 and Muhammad Sajjad Khan1

...67 indicating very lower genetic control. This is the first study to show genetic of persistency of lactation. Although results may be validated using more data and more accurately recorded pedigree for several generations however, this is really useful firsthand information.
...
Waqar Islam*1, 2, Madiha Zaynab3, Muhammad Qasim2 and Zujian Wu1,2
... involving cis and trans genetics. The review adhere recent research findings regarding disease resistance against viral diseases and concludes via shedding light upon the future prospects in this exciting field of research.
...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...otyping of SCARB1 genetic polymorphisms was done by PCR-RFLP combined with gel electrophoresis. The genotype distribution in the population was found to be consistent with Hardy Weinberg equilibrium. Through logistic regression analysis, it was observed that, in Tamil population the CC and CT genotype carriers have higher odds of developing myocardial infarction (OR – 1.64 and  2.04 respectively) and for developing hypercholesterolemia (OR &n...
Tianzhu Chao, Huiqiang Cai, Yuxun Zhou, Kai Li* and Junhua Xiao
...gated the molecular phylogenetics of Chinese house mice based on 466 DNA sequences from mitochondrial D-loop fragments. Our analyses revealed that Mus musculus musculus and M. m. castaneus are older colonized subspecies with broad distributions in East China, while M. m. domestics is a newly arrived subspecies currently restricted to Shanghai, indicating the elevated risk in this cosmopolitan cities. Phylogenetic
Jun Gao1,2, Liang-Liang Yue3, Xianhuan Jiang1, Liju Ni4, Muhammad Aqeel Ashraf5, Yuxun Zhou,1 Kai Li1,* and Junhua Xiao1,*
... habitat has resulted in genetically distinct populations. The trees, inferred from maximum likelihood and Bayesian phylogenetic analysis, highly supported all the M. fortis individuals clustering into one monophyletic lineage. Three main clades are recovered within M. fortis: (1) North group; (2) South group; and (3) GX group. The North Group distributed on the north side of Qinling Mountains-Huaihe river line...

Muhammad Noor, Hidayat ur Rahman and Muhammad Iqbal

...ariability was mostly of genetic nature and hence, could be effectively utilized in maize breeding programs for developing popcorn varieties having early maturity and accompanied with other desirable attributes.

...
Urooj Fatima1, Arslan Mehboob2, Muhammad Abid3, Muti-ur-rehman Khan4 and Tahir Yaqub4*

 

...he current situation and genetics of CPVs in Pakistan, a total of fifty samples from sick dogs suspected for parvovirus infection were collected from different geographic areas of the country. Samples were screened for positivity and verified for CPV using PCR. From a 30% positive samples for hemagglutination, nineteen (38.4%) samples were positive in PCR. Sequence analysis of the selected positive samples revealed that both CPV-2 and new CPV-2a strains are ci...

K.N. Ahmed and M.R. Hasan

Sudden outbreak of mealy bug and armoured scale causing severe damage to economic crops in Bangladesh
... females develop parthenogenetically. It is a sucking pest and mainly feeds on citrus. Its secondary hosts include fig, apple, almond, guava etc. Due to severe infestation, premature falling of leaves occurs. The ber or plum scale, Aonidia ziziphi Rah. was recorded from Rajshahi, Bangladesh as a serious pest of ber or plum plant damaging the leaves and immature fruits during December-January, 2007. The female reproduces parthenogenetic...

Bijan Kumar Das

Incidence of Aleurocanthus spp. (Aleyrodidae: Hemiptera) on betelvine (Piper betle L.) and their interaction with host plants
...sts is very relevant for genetic improvement programmes. Till date, scanty information is available on the source of tolerance, if any, against betelvine blackfly. The reaction of some betelvine cultivars to A. rugosa was evaluated in the boroja with different varietal collections from different parts of India. None of the entries under the purview was completely free from infestation. A few cultivars [CARI- 2 (AN), CARI-6(AN) and Bilhari] exhibited moderate r...

M. S. A. Mamun and M. Ahmed

Integrated pest management in tea: prospects and future strategies in Bangladesh
...ltural control measures, genetic techniques and sex pheromones technology. In tea husbandry, cultural control measures such as plucking, pruning, shade regulation, field sanitation, fertilizer application, manipulation or destruction of alternate hosts and selection of pest resistant/tolerant varieties and mechanical mechanisms like manual removal, heat treatments, light traps, use of bio-pesticides, bio-control agents and sex pheromone trap need to be given m...

Rashid Pervez, Santhosh J. Eapen, S. Devasahayam and M. Dinsha

Characterization of entomopathogenic nematode, Stein-ernema carpocapsae from ginger (Zingiber officinale Rosc.) rhizosphere in India
...sequences analysis. Phylogenetic was constructed for studying relationship with known isolates. Pathogenic potential of the isolate of S. carpocapsae (IISR-EPN 06) on the larva of shoot borer (Conogethes punctiferalis), hairy caterpillar (Euproctis sp.) and greater wax moth (Galleria mellonella) was found under in vitro condition. Further this isolate displayed high virulence on above insect species. This study reported occurrence of a isolate of S. carpocapsa...

A. K. Ganguly and Uma Rao

Decades of researches in biochemical and molecular nematology at IARI
...d to study the molecular genetic diversity in major root knot and cyst nematode species of India to assist the breeding program for developing resistant varieties and other purposes like regulatory and nematode diagnostics. Attempts have also been to made to understand the molecular signaling events during compatible plant nematode interactions by identifying the genes during the transition from preparasitic to parasitic stage in ear cockle nematode and molya ...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
...s trigger the disease in genetically sensitive individuals. Gluten, a protein fraction commonly found in wheat grains, associated with food related disorders and a number of autoimmune diseases. We hypothesized that gluten containing diet would further exacerbate an already undergoing arbitrary immune reaction in SLE patients. Pristane was injected in female BALB/c mice to induce the disease. After five months, mice in various groups were treated with predniso...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1 
...is a first report toward genetic screening of this gene at molecular level in Sahiwal cattle of Pakistan. No work has been reported on this gene in Sahiwal cattle. In this study, a new set of single nucleotide polymorphisms (SNPs) were reported. Heterozygosity, allelic and genotypic frequencies of identified variants were also determined. Chi2 test was performed to evaluate the Hardy-Weinberg Equilibrium of each polymorphic site. Two loci were found...
Ayesha Zahid,Ammara Muazzam, Sidra Mustafa, Saba Irshad*,Malik Siddique Mahmood and Rehman Shahzad

 

...ion in such patients but genetic researches have validated that, mutations in GJA8 are coherent source of lens opaqueness and inappropriate growth of fiber cells. In the present study, a novel G to C substitution (1104G>C) (pE368Q) was screened by PCR-SSCP in exon 2 of GJA8 and this tansversion altered exceedingly conserved glutamic acid to glutamine at site which was involved in coding of ASF1 like histone chaperone. Further presumption based on str...

Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1 

...T34, S99 and P199). Phylogenetic analyses showed that the GPCR genes of LSDVs identified from ticks were closest genetically to the previously detected LSDVs from infected ruminants, indicating a potential role of Ixodid ticks for transmission of LSDV. This study showed the role of A. hebraeum ticks for transmission of LSDV. So, tick control is a crucial part, which should be included as a part of LSDV control measures in en...
Süleyman Kök*
... to compare 3 SNP of the genetic features of purebred TGC that are under the conservation of ex-situ (51 cattle) program with the ones raised by the smallholders (79 cattle) in the villages. According to the estimated average heterozygosity values for ex-situ breeding program and smallholders in the villages, the difference between the cattle were found meaningful (P<0.05). The observed average heterozygosity (Ho) value was calculat...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...enzymes with a wide phylogenetic distribution. They are multifunctional enzymes that play a crucial role in cellular detoxification and oxidative stress tolerance. Comparing with that in mammals, investigation of GSTs is more complicated in teleosts because of the greater pressure they suffer in aquatic environment. In this study, we identified a set of 27 GSTs including 8 classes of members in common carp genome. Both sequences alignment and phylo
Javeria1, Masroor Ellahi Babar2,*, Tanveer Hussain2, Rashid Saif2, Sadaf Rashid3, Hanan Sarfraz4, Abbas Ali Shah3, Muhammad Wasim1 and Muhammad Abdullah1
...udy is aimed to identify genetic variants in BDNF gene and their association with Obsessive Compulsive Disorder (OCD) among Pakistani Punjabi population. We recruited a total of 100 patients diagnosed with OCD following Diagnostic Statistical Manual-IV (DSM-IV) criteria and controls (n=120) were selected from the same ancestry during August 2011 to January 2014fromSir Ganga Ram Hospital and Panjab Institute of Mental Health, Lahore. The demographic valu...
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
...e to environmental or epigenetic effect, sampling number or a breed difference. To know the functional consequences of the identified polymorphism, RNA secondary structure analysis was performed and SNP was found changing the termination loop shape in mRNA. However, to increase the selection efficacy of dairy animals, the effects of candidate polymorphism should further be screened in different environments and genetic backg...
Muzafar Shah1,*, Mian Sayed Khan1, Muhammad Ather Rafi2, Habib Ahmed3 and James M. Carpenter4
...mily of Polistinae. Phylogenetic tree were reconstructed using 12 SrRNA (12S) gene showing homology of 73% compared with gene bank data while on the basis of cytochrome oxidase 1 (Co1) gene 93% homology were recorded. It was concluded that Co1 gene is more reliable than 12S rRNA gene for confirmation of R. brevita position in the Vespidae family.
...
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
Muhammad Imran Shabbir
...;">The heart of learning genetics is in realizing that how the likelihood of inheriting a particular trait can be predicted. This fundamental approach is the basis for plant and animal breeding to get desired varieties as well as performing genetic analysis including prediction of patterns of inheritance in family lines and to calculate the recurrence risk for relatives etc. Punnett square is used to describe the possible co...
Hassen Allaya1,2,*, Abderraouf Ben Faleh1, Manel Rebaya1, Sonia Zrelli3, Abdallah Hattour2, Jean-Pierre Quignard4 and Monia Trabelsi1
...caused by differences in genetic structure or environmental conditions.
...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
...for construction of phylogenetic tree and genetic diversity analysis. Lower genetic diversity was observed. The finding of this research is prerequisite for future research.
...

Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4

...ed to determine the phylogenetic relationship of currently prevailing H9N2 isolates in Pakistan. A sum of ten H9N2 AIVs were isolated from 400 suspected samples and were confirmed through serological and molecular assays. The sequence analysis of NA genes shown 99% homology with the H9N2 AIV recently isolated from Pakistan and their phylogenetic analysis revealed that all the isolates belonged to the G- lineage. Amino acid a...

Attaullah Khan1, Muhammad Shafi1*, Jehan Bakht2 and Shazma Anwar1

Fareeda Tasneem1, Farah Rauf Shakoori1 and Abdul Rauf Shakoori2,*

 

...pecies, a molecular phylogenetic analysis was performed on the basis of H4 gene sequences. A phylogenetic tree was constructed using the aligned sequences of our study with 22 entries of closely related species from GenBank Data. Highly polymorphic sites were observed in FT8 strain sequence as compared to other species. Analysis of H4 gene sequences in our study showed they are closely related and behaved as a good substitut...
Mehran Kausar1,2, Naveed Ashraf3, Farzana Hayat4, Asraf Hussain Hashmi1, Saima Siddiqi1,* and Mariam Anees2
...ycnodysostosis is a rare genetic bone disorder (OMIM 265800) with an autosomal recessive mode of inheritance. More than 50 mutations have been reported in CTSK (Cathepsin k) responsible for this disease. Mutations in CTSK result in impaired bone resorption consequently leading to short stature, increased bone density, recurrent fractures, stubby hands and feet with dystrophic nails, unossified fontanels, and an obtuse mandibular. The present stud...
Lu Liu1, Sher Khan Panhwar2, Tianxiang Gao3, Zhiqiang Han3, Chunhou Li4, Dianrong Sun4 and Na Song1,*
... The mean within-species genetic distances for L. affinis, L. carinata, and L. klunzingeri were 0.26%, 0.52%, and 0.47%, respectively. The mean genetic distances among L. affinis, L. carinata, and L. klunzingeri ranged from 12.22% to 14.74%, higher than the threshold for species delimitation. The results confirmed the taxonomic validity of the three Liza species and verified t...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,
...In order to evaluate the genetic diversity and population structure for the conservation and management of sables (Martes zibellina), we developed six microsatellite markers using a method of fast isolation by AFLP of sequences containing repeats (FIASCO) from them in this study. The primers designed by Primer Premier 5.0 based on conservative regions were tested on 22 individuals. Our results demonstrated that the number of alleles per locus ranged fro...
Zuhao Huang*, Meifang Li, Rujue Ruan and Jiawei Qin
...llaris). The average genetic distances among the species varied from 5.39% (between Egretta sacra and Egretta garzetta) to 41.15% (between Egretta sacra and Botaurus stellaris). Five conserved sequence boxes (F to B) in the domain II of Ardeidae sequences were localized. Maximum likelihood method was used to reconstruct the phylogenetic trees based on HKY+ G model. The phylo
Javeria1, Masroor Ellahi Babar1,2,*, Akhtar Ali2, Asif Nadeem1, Abdul Wajid1, Sajjad Ali Shah3, Sadaf Rashid4, Muhammad Wasim1 and Muhammad Abdullah1
...tudyaimedto find out the genetic variations in Catechol O Methyl transferase (COMT) gene and association of these genes with obsessive compulsive disorder (OCD) in the Pakistani Patients. We selected OCD patients (n=100) following the Diagnostic Statistical Manual-IV (DSM-IV) criteria and controls (n=120) from Sir Ganga Ram Hospital and Panjab Institute of Mental Health, Lahore from August 2011 to January 2014. During the sample collection...

Rabia Tahir1*, Khushi Muhammad1, Masood Rabbani1, Muti-u-Rehman Khan2, Farah Khan1, Hassan Bin Aslam1 and Arslan Mehboob

...tions. However, based on genetic detection such evidences are lacking. The molecular mechanism and establishment of latent infections warrant future investigations to elucidate these discrepancies.

...

Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali

...ed to LBA4404. The maize genetic transformation protocol developed in this study will possibly improve the efficiency to produce new transgenic maize lines expressing desirable agronomic characteristics. 

...

Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali* 

... a starting material for genetic transformation. Unlike immature embryos, the development of coleoptilar node is seasonal free and green house independent which would accelerate the work relevant to genetic transformation of maize. MS medium fortified with 3 mgL-1 BAP and 5 mgL-1 picloram produced maize seedlings with expanded coleoptilar nodes. Longitudinal cut sections of expanded coleoptilar nodes produced maximum primary...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
...AR Lasergene. After phylogenetic analysis with 3 clonal types and atypical strains, and on the basis of restriction map of HhaI and Sau3AI, our 3 isolates of T. gondii were found more closely linked to a typical strain. This is the first genetic analysis of T. gondii in Pakistan. In order to develop our knowledge about the toxoplasmosis epidemiology, further genotype analyses of T. gondii from animals an...
Rabia Arif*, Shahar Bano, Muhammad Ishfaq and Muhammad Saleem
...fimicola, to examine genetic diversity. Strains were taken from two contrasting environments (Natural and Harsh) from the south facing and north facing slopes of Evolution Canyon, Israel. Sequenced amplicons were introduced to the NCBI database as a query in BLASTN. The query revealed >100% resemblance with the Ascomycota genus Sordaria and the topmost species in this investigation was fimicola. Circular Phylogram showed close relatedness ...
Mahmood-Ul-Hassan*, Naeem Fiaz, Muhammad Akhlaq Mudassir and Muhammad Yasin
...s for most of traits and genetic potential of genotypes. It was also found that the ratoon crop yield was highly dependent on the number of sprouts which were significantly varied among harvesting dates and the genotypes tested as well (R2=0.998 & 0.994, respectively).
...
Liana Mihaela Fericean1* and Mihaela Corneanu2
...phids gave birth parthenogenetically to three generations of fundatrigenous. In July, the winged form migrated to the secondary host, where successive generations have been born through parthenogenetic reproduction. Gynopara in October return to the primary host and give birth to wingless oviparae that mate with males and lay winter eggs. The biometrical measurements of Aphis nasturtii help to complete knowledge on ex...

Amjad Ali1*, Sher Aslam Khan2, Abid Farid2, Ayub khan2, Shah Masaud Khan2 and Naushad Ali2 

...ability (repeatability), genetic gain and correlations among the parameters, and establish selection criteria.Highly significant (p ≤ 0.01) differences were found among the genotypes for number of tillers, plant height, cane length,number of nodes, internodes length,number of millablecane and cane yield while non-significant differences were recorded for cane diameter. The effect of genotype and crop interaction was highly significant fornumber of tillers w...

Arshad Iqbal1*, Iftikhar Hussain Khalil1, Syed Mehar Ali Shah1 and Muhammad Sharif Kakar

...gh heritability with low genetic advance was estimated for days to maturity, while moderate to high heritability coupled with genetic advance was recorded for other yield related traits. Correlation analysis showed that grain yield had strong positive phenotypic and genetic correlation with heading, maturity, flag leaf area and biological yield. Spring wheat genotype SM12 had best performa...
Tahira*, Muhammad Arshad, Muhammad Ayub Khan and Mubashar Ahmad Khan
...thod showed considerable genetic variation among the hybrids. In the first year, thirty- six Brassica hybrids were grouped into thirteen clusters. Cluster-IV comprised of maximum number of hybrids (six). The clusters mean and standard deviation marked that hybrid in Cluster XI (CRH-80) was short durational and high yielding mainly due to more branches and pods per plant.CRH-35 in cluster-VI was high yielding and has more seeds per pod. Similarly in second year...
Farid S. Ataya,1,2,* Dalia Fouad,3,4 Ajamaluddin Malik,1 Nikolaos E. Labrou,5 Mohamed S. Daoud1,6 and Hesham M. Saeed7
...an GSTP1-1 enzymes. Phylogenetic relationship was studied with different organisms belonging to animal kingdom and revealed that CdGSTP1-1 is grouped with the enzyme from S. scrofa. The 3D homology model of CdGSTP1-1 showed similar fold and topology with the porcine GSTpi enzyme. Gene expression analysis in five camel tissues was examined employing real-time PCR. The highest level of transcripts was found in the camel testis, followed by l...

Ehtisham Shakeel Khokhar1*, Amir Shakeel1, Muhammad Amir Maqbool1, Muhammad Waheed Anwar1, Zoraiz Tanveer1 and Muhammad Fahad Irfan2 

...carried out to study the genetic variability, coefficient of variations, heritability, genetic advance and correlation among 20 F1 hybrids and 9 parents in upland cotton. Line × tester mating design was deployed to develop crosses and 29 cotton genotypes were field planted in randomized complete block design (RCBD) in three replications to assess the genotypes for plant height (cm), number of sympodial branches, number...
Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*
...culated and computed for genetic associations presented as odds ratios (ORs) with a threshold of p≤0.05 to determine statistical significance. The frequency of 54Thr allele of FABP2 significantly associated with MetS risk in Pakistani population. Our results showed that frequency of the FABP2 54Thr allele was significantly higher (67%) in MetS cases as compared to controls (35%)(p<0.0001). The 54Thr allele carriers were at increased ...
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
...-Fresian cattle there is genetic variety in analysed locus c.1178G>A gene PIT-1. Frequency of genotypes and alleles was respectively: AA=0.0469, AG=0.3154, GG=0.6377, A = 0.2046 and G = 0.7954. AA homozygotes had the highest age at first insemination and the lowest insemination index. Cows with this genotype after calving and calves (both gender) from AA homozygotes had the biggest weight after calving. In contrast GG homozygotes had th...

Warda Fatima1 , Shahida Hasnain1 , Saqib Mahmood2

 

...hether it’s a pure genetic disease. It is considered a polygenic multifactorial condition where both genes and environment have roles in disease development. In the present study we selected a family withmultiple schizophrenic patients and have identified some reasons for disease development. It is estimated from study that in Pakistan there is gender bias in schizophrenia unlike the worldwide data because more of males are affected in the selected famil...
Yunjun Yan, Zhenming Lü, Tianming Wang, Yongjiu Chen, Jingwen Yang, Baoying Guo, Lihua Jiang, Changwen Wu and Liqin Liu*
...a, and analyzed the phylogenetic relationship with other cephalopod species. The results show that the mitochondrial genome of O. dollfusi composed of 15843 nucleotide pairs and encodes 13 proteins, 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs) and a major long noncoding region (LNCR) of the mitochondrion’s own protein synthesizing system. Seven of thirteen proteins, eight tRNAs are encoded by the plus strand, while the other proteins and tRN...
Tania A. Shakoori1,*, Soumble Zulfiqar2, Khadija Irfan Khawaja3 and Faisal Masud4
...ot reported. We compared genetic expression of Sirt1, AMPK, Akt, and mTOR in adipose tissue from mice treated with NaB versus placebo. Four groups of mice (n=27) mice were administered oral NaB, intraperitoneal (i.p) NaB, oral placebo or i.p placebo for 16 days. RNA was isolated from the epididymal fat pads and relative genetic expression of AMPK, Sirt1, mTOR and Akt was measured using real time PCR. Oral NaB group showed de...

Syed Asif Imran Shah1*, Shah Jehan Khan2, Kalim Ullah1 and Obaid Ullah Sayal

...es were investigated for genetic divergence regarding yield related attributes, seed cotton yield and fibre quality traits using principle component analysis. The experiment was carried out at Cotton Research Station, Deara Ismail Khan, Pakistan during 2014-15. The triplicated randomized complete block design was followed and attributes of interest were plant height, monopodia plant-1, sympodia plant-1, bolls plant-1, plant population, seed index, boll weight,...
Muhammad Abubakar,1,2,* Aamer Bin Zahur,1,3 Khalid Naeem1,3, Muhammad Azeem Khan1,3 and Subhan Qureshi
... The results of the phylogenetic tree indicated that all Pakistani PPRV strains, regardless of the gene used either F or N, clustered in lineage IV which is the most prominent and prevalent lineage of Asia. The distribution of Pakistani strains of PPRV was more dispersed as the isolate collected from Taxilla was clustered slightly distinct compared to rest of the isolates collected from Pakistan. Our findings are indicative of PPR endemic state of the country....
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
...was planned to study the genetic diversity in the M. tuberculosis isolates isolated from clinical samples. In the current study total 794 patients were tested for suspected tuberculosis by fluorescence microscopy and GeneXpert system during the study period (2015-2016) at the Provincial TB Reference Laboratory, Khyber Pakhtunkhwa, Pakistan. Among the 794 patients studied, 170 samples gave positive results. Fifty-two Mycobacterium tuberculosis com...

Zahid Akram, Saif Ullah Ajmal, Ghulam Shabbir, Muhammad Munir and Nasir Mahmood Cheema

INHERITANCE MECHANISM OF SOME YIELD COMPONENTS IN BREAD WHEAT
...alysis. The estimates of genetic component of variation in all the traits exhibited the preponderance of
over dominance gene action. The estimates of average degree of dominance for spike length (1.139), 1000 grain weight (1.421), number of spikelets spike-1 (2.293), number of grains spike-1 (1.631) and grain yield plant-1 (1.111) evidenced the prevalence of non additive gene action controlling these traits. The present results, therefore, revealed...

Raania Ahsan* and Zafar Altaf**

...an to introduce advanced genetically modified (GM) crop technology in Pakistan by Government of Pakistan during 2009. In this regard Ministry of Food and Agriculture (MINFA) has been working on a two pronged strategy i.e. developing the technology through indigenous capabilities as well as inviting the multi-national companies (MNCs) to bring in the latest cotton production and protection in the country. Bt cotton can play a significant role to enhance agricul...

 Z. A. Soomro, M.B. Kumbhar, A.S. Larik*, M. Imran** and S.A. Brohi***

...nbsp;Heritability (b.s), genetic advance, phenotypic and genotypic variance for different quantitative traits is important in determining the effectiveness of selection. In this study the potential effectiveness of selection in parents and their F2 and F3 progenies of Gossypium hirsutum for plant height, sympodia/ plant, bolls/plant, boll weight, seed index and seed cotton yield/plant was determined. It was found that all the populations alongwith parents diff...

 Oguz Bilgin, Kayihan Z. Korkut, Alpay Balkan, and Ismet Baser*

...amme. Highly significant genetic variability was present in parents and their genetic combinations for the characters. The degree and direction of heterosis varied for different characters and for different hybrids. The highest heterosis over mid and better parents (120.14-109.93%) was recorded in hybrid of Kyzyltan91xIDSN209 for grain weight/spike. Maximum limits of heterosis over mid and better parent for spike length, spi...

 M. Yasin Mirza, Mubashir A. Khan, M. Akmal, Akbar S. Mohmand, Malik S. Nawaz, Nazakat Nawaz and Najeeb Ullah*

...onding high estimates of genetic advance in percent of mean (17.23 and 18.33) for these traits indicated the predominance of additive gene effects. Mass selection based on these additively controlled traits is suggested for improvement of yield. Plant height, number of bolls plant-1 and 1000-seed weight showed genetic advance of 7.96, 8.85 and 17.23 % respectively with the prospects of improving seed yield up to the level of...

 Shazia Erum*, Muhammad Naeemullah, Shahid Masood** and Muhammad Irfan Khan*

...s of total seed proteins genetic distances ranged from 0.11 to 1.00. Due to greater genetic diversity in Ocimum germplasm and its suitability for commercial cultivation even in the area under small holdings, the investigation suggest its genetic as well as biochemical investigation for the production of commercial varieties and exploitation of the plant for economic benefits of the local c...

Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2* 

...These findings show some genetic features of CCoV in Brazil and may require future studies to elucidate full genome sequences of these isolates to better assess the disease transmission dynamics and future control strategies. 

...
Saba Rafique1,2,*, Khalid Naeem1, Naila Siddique1, Muhammad Athar Abbas1, Aamer Ali Shah2, Akbar Ali1, Abdul Rahim1 and Farooq Rashid1
...on number KU145467. Phylogenetic grouping and maximum nucleotide sequence identity values were used to identify the isolate that looked to be derived from recombination. It showed maximum nucleotide homology 99.5% with ck/CH/LHB/121010 (KP036503), India/IBV572 (KF809797) and Japan/JP/Wakayama-2/2004 (AB363951.2) and 99.3% with 4/91 vaccine (KF377577), Iran/491/08 (HQ842715) and 99.1% with India/NMK/72/VRI/10 (HM748585) and was least related to rest of IBV line...
Haixia Liu, Yuwei Ye, Yang Li, Xiaolin Liu, Dongmei Xiong* and Lixin Wang
...m the two rivers and the genetic diversity of wild population, 11 microsatellite loci were firstly used to evaluate the genetic characterization using 120 samples from 2 tributaries (ZZ and LX populations) of the Weihe river and 2 tributaries (YX and TB population) of Hanjiang river. A total of 59 alleles were found and the number of alleles in each locus ranged from 3 to 8, with an average of 5.4. There were 6 highly polymo...
Muhammad Rasool1,*, Muhammad Zahid1, Muhammad Ismail Khan1, Qadeem Khan2 and Khalid Khan1
...no acid change. The phylogenetic studies established that the Pakistanian HBV Isolates closely resemble the “adrq” strain belonging to “adr” subtype. The study showed that the pre-S2 region is hypervariable and two reported sequences show 100% homology and thus cause change in the structure of Hepatitis B Surface Antigen (HBsAg). This variability reduces the efficacy of vaccines prepared to a particular clone of HBV isolates.
...
Lin Wang1,2, Ye Gong3, Kelin Chen1, Haitao Wang3 and Xianguo Lyu1,*
...nce our understanding of genetic information and breeding biology of Relict Gull.
...

 Javid Iqbal, Asghar Ali Mian, Tanveer Ahmad*, Shamsul Hassan** and Sohail Hassan Khan* 

COMPARATIVE PERFORMANCE OF DIFFERENT ECONOMIC TRAITS OF FOUR IMPORTED BROILER STRAINS UNDER LOCAL CONDITIONS OF PAKISTAN
... remain abreast with the genetic improvement of broiler strains. 

...

 Muhammad Yaqoob*, Rukhsana Anjum, Mumtaz Hussain and Muhammad Jahangir Shah**

GENETIC DIVERSITY ANALYSIS AND CHARACTER ASSOCIATION IN SOME CHINESE HYBRID RICE UNDER DRY CONDITIONS
...ndicated a high range of genetic variation among various hybrids for plant height, number of grains per panicle, number of productive tillers per plant, 1000 grain weight and paddy yield. The variation for days to 50% panicle initiation, panicle length, spikelet fertility and days to maturity were however nonsignificant. Plant height ranged from 77 to 116 cm, number of grains per panicle from 152 to 449, number of productive tillers per plant from 15 to 29, 10...

 Muhammad Yasin Mirza*, Mubashir Ahmad Khan*, Muhammad Amjad* and Malik Shah Nawaz*

STABILITY ANALYSIS FOR ECONOMIC TRAITS IN SESAME ( L.)
...e of genotypes was under genetic control. The b-values of V-90005, T-89 and PARS-I were larger than unit regression; hence were suitable for favourable environments for yield. Whereas, V-III and SangharI were with b-values less than unity indicating their below average response. For branches per plant, Sanghar-1 and S-17 had regression coefficients less than one with negative sign making them suitable only for poor environment. Four genotypes namely, Sanghar-I...

 Mubashir Ahmad Khan*, Muhammad Yasin Mirza*, Muhammad Amjad*, Nazakat Nawaz*, Malik Shah Nawaz* and Doulat Baig*

ASSESSMENT OF GENETIC DIVERSITY IN GERMPLASM OF LINSEED ( L.)
...the existence of greater genetic diversity in the accessions for these traits. Dendrogram based on Euclidean distance coefficient using 10 quantitative traits, grouped all the linseed accessions into 13 clusters. Cluster II was the biggest and had 33 accessions followed by Cluster I having 11 accessions. For the development of high yielding varieties, best performing accessions of Clusters I and II could be used in hybridization programme by crossing with acce...

 Md. Nazirul Islam*, A. K. Azad**, Leon O. Namuco***, Teresita H. Borromeo***, Maria Lourdes O. Cedo*** and Edna A. Aguilar***

MORPHOMETRIC CHARACTERIZATION AND DIVERSITY ANALYSIS OF A MAKAPUNO COCONUT POPULATION IN U.P. LOS BANOS

 Said Salman*, Shah Jehan Khan*, Javed Khan**, Rehmat Ullah Khan*** and Imran Khan****

GENETIC VARIABILITY STUDIES IN BREAD WHEAT (TRITICUM AESTIVUM L.) ACCESSIONS
...y estimates and expected genetic advance were found for all the traits except chlorophyll concentration index, spike length and number of -1 spikelet spike which exhibited moderate heritability. Based on Euclidian dissimilarity distance, 65 wheat accessions were classified in to 6 different clusters. Maximum diversity was found in cluster 1 and cluster 4. This maximum diversity explains the better parental selection for future breeding programme.

...

  Abdul Hassan*, Muhammad Ishaq*, Nisar Ali Shah* and Arshad Farooq*

MILK PRODUCTION POTENTIAL IN KHYBER PAKHTUNKHWA
...by proper management and genetic improvement. More research is needed at farm level in the context of breed adaptability and management in respect of cost of livestock herds production in Khyber Pakhtunkhwa, Pakistan.

...

 Muhammad Sohail*, Imtiaz Hussain*, Riaz-ud-Din*, Sikandar Khan Tanveer*, Maqsood Qamar* and Syed Haider Abbas*

EVALUATION OF ADVANCE WHEAT LINES FOR AGRONOMIC TRAITS IN RAINFED ENVIRONMENT
...397 and NARC-09 have the genetic potential to tolerate adverse rainfed growing conditions under agro-ecological conditions of Pothwar region, Pakistan.

...

 Md. Nazirul Islam*, M. Z. Rahman**, R. Ali***, A. K. Azad**** and M. K. Sultan**

DIVERSITY ANALYSIS AND ESTABLISHMENT OF CORE SUBSETS OF HYACINTH BEAN COLLECTION OF BANGLADESH

 Syeda Nasreen*, Mehwish Ishaque**, Muhammad Ayub Khan*, Saleem-ud-din* and Syeda Musarrat Gilani*

COMBINING ABILITY ANALYSIS FOR SEED PROTEINS, OIL CONTENT AND FATTY ACIDS COMPOSITION IN SUNFLOWER (HELIANTHUS ANNUUS L.)

 Frasat Saeed*, Jehanzeb Farooq*, Abid Mahmood**, Tassawar Hussain***, Muhammad Riaz* and Saghir Ahmad****

GENETIC DIVERSITY IN UPLAND COTTON FOR COTTON LEAF CURL VIRUS DISEASE, EARLINESS AND FIBER QUALITY
...LCuD). For estimation of genetic diversity regarding CLCuD tolerance, fiber quality and some yield contributing traits, 101 cotton genotypes imported from USA were evaluated. Different statistical procedures like cluster, principle components (PC) and correlation analysis were employed to identify the suitable genotypes that can be further exploited in breeding programme. Significant associations were found between yield contributing trait, boll weight and fib...

 Muhammad Ali*, Ghulam Mustafa Sajid**, M. Faisal Anwar Malik*** and Kalimullah****

ESTABLISHMENT OF IN VITRO CULTURE OF GRAPES

 Rashid Minhas*, Iftikhar Ahmad Khan**, Faisal Saeed Awan**, Lal Hussain Akhtar*, Syed Awais Sajid Shah* and M. Shahjhan Bukhari* 

ESTIMATION OF GENETIC DIVERSITY AND HYBRID IDENTIFICATION IN AMERICAN COTTON (GOSSYPIUM HIRSUTUM L.) BY PCR BASED RAPD ANALYSIS
...ure leaves to estimate 1 genetic diversity and hybrid identification. Out of the 26 RAPD primers used for DNA amplification, 14 primers produced polymorphic bands, while 12 produced monomorphic bands. These 14 primers yielded total 96 RAPD markers, out of which only 40 were found polymorphic which resulted in 41.66% polymorphism among the genotypes studied. The number of RAPD markers produced ranged from 4 to 12. Average number of 6.40 bands per primer was obs...

 Kalim Ullah*, Zahid Usman*,Niamatullah Khan*, Rehmat Ullah**, Fazal Yazdan Saleem***, Shahid Iqbal Khattak**** and Muhammad Ali*****

GENETIC DIVERSITY FOR YIELD AND RELATED TRAITS IN UPLAND COTTON GENOTYPES
...periment to evaluate the genetic variability and yield potential in cotton cultivars was conducted at the Cotton Research Station, D.I.Khan, Pakistan, during 2013. The experiment was carried out in Randomized Complete Block design replicated four times. The genetic material comprised nine cotton cultivars viz., Gomal-93, SLH-373, BH-186, BH-172, CIM-573, CRIS- 342, CIM-591, CIM-608 and DNH-105. Results depicted the significa...

 Syed Asif Imran Shah*, Shah Jehan Khan**, Abdul Aziz Baloch*** ,
Obaid Ullah Sayal**, Kalim Ullah* andShujaat Ali**

COMBINING ABILITY ANALYSIS IN VARIOUS INBRED LINES OF MAIZE (ZEA MAYS L.)
...to dominant and additive genetic background, respectively.

...

 Israr Ali*, Naushad Ali*, Riffat Tahira**, Sardar Ali*, Izhar Hussain* and Sher Aslam Khan*

GENETIC DIVERSITY ASSESSMENT IN BRASSICA GERMPLASM BASED ON MORPHOLOGICAL ATTRIBUTES
...es that this genotype is genetically different and superior than the other studied genotype. Therefore, genotype Kalabat can be either used as variety after adaptability trials over a larger area or included in Brassica breeding programmes as a good source of genetic variation. 

...

 Muhammad Mohsin Raza*, Muhammad Aslam Khan*, Irfan Ahmad**, Ali Ahsan Bajwa***, Hafiz Muhammad Usman Aslam*, Badar Ahsan Ullah****, and Kashif Riaz*

FOREST PATHOGENS AND DISEASES UNDER CHANGING CLIMATE -A REVIEW
..., risk rating, planning, genetic diversity and facilitated migration, genetic protection and breeding for disease resistance and relating results to forest policy, planning as well as decision making, the suspicions innate to climate change effects can be minimized.

...

  Sohaib Arshad*, Sarwat Naz Mirza*, Imtiaz Ahmad Qamar** and Maqbool Shahbaz**

DETERMINATION OF FORAGE QUALITY OF INDIGENOUS AND EXOTIC RHODES GRASS ACCESSIONS UNDER RAINFED CONDITIONS
...d be attributed to their genetic inheritance potential. Mean value of crude protein content (CP) in three grasses varied from > 12% to < 5% depending upon the different growth stages of the grasses. Sabre showed highest CP content than other two at flowering stage. The results also showed that protein contents were lowest at the maturity stage of all the experimented grasses. This study has shown that Katambora is the variety which can be adapted to Paki...

 Ishaq Ahmad*, Abdul Rehman**, Ehsan –Ul- Haq*** and Arif Mahmood****

RESISTANCE RESPONSE OF RICE ( L.) GERMPLASM AGAINST RICE LEAF FOLDER ( G.) UNDER GREENHOUSE CONDITION IN PAKISTAN

Basharat Hussain Shah*, F. S. Hamid*, Shams ul Islam*, Fayaz Ahmad*, Sohail Aslam*, and Noorullah Khan*

EVALUATION OF DIFFERENT PEA (PISUM SATIVUM L.) GENOTYPES FOR YIELD AND OTHER ATTRIBUTES AT SHINKIARI, MANSEHRA
...o find out the amount of genetic variability that may exist in different pea cultivars. For this purpose six advance lines/commercial pea varieties were collected from Ayub Agricultural Research Institute (AARI) Faisalabad and one local variety was included in the experiment as a control. The experiment was conducted during 2014-15 at National Tea & High Value Crops Research Institute (NTHRI) Shinkiari, Mansehra. Altogether seven pea genotypes were laid ou...

 

Shakir Ullah*, Aish Muhammad*, Iqbal Hussian*, Hafeez-Ur-Rahman**, Muhammad Zeeshan Hyder***, Muhammad Din**** and Nizamud Din

MORPHOLOGICAL VARIATIONS IN APRICOT CULTIVARS GROWN IN GILGIT BALTISTAN PAKISTAN

 Nasarullah*, Raiz uddin*, Fakhar Uddin* and Muhammad Jamal*

INTERPRETING WHEAT GENOTYPES FOR GRAIN YIELD UNDER DIFFERENT REGIMES OF KHYBER PAKHTUNKHWA
... three replications. The genetic material was comprised of 9 nationally released cultivars, three advance line from Tajikistan, one from China and two locally grown cultivars. The analysis of variance revealed significant differences among genotypes, locations and genotype by environment interactions for days to heading, days to maturity, plant height (cm), spike length (cm), spikelets spike , 1000-grain weight and grain yield. Flag leaf area (cm displayed non...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
...A, S336N). The HA and NA genetic evolution analysis revealed that one isolate was most similar to the newly isolated H3N2 viruses from dogs in China and had the same evolutionary branching. The result provided a foundation for further studies on biological characteristics of CIV.
...
Yanling Xia1,2, Heping Li2, Yuntao Liang2, Jichen Zhao2, Binshan Lu2 and Di Liu1,*
...uence alignment and phylogenetic tree analysis indicated that theVps26A protein of sika deer was highly similar to that of Bos taurus. Expression analysis by real-time quantitative RT-PCR revealed that Vps26A gene had a higher expression level at day 90 than those obtained at day 60 and 30.
...
Ali Haider Saleem1,*, Khalid Javed2, Masroor Ellahi Babar3, Tanveer Hussain3, Asad Ali2, Afzal Ali2, Nisar Ahmad2, Muhammad Zahid Farooq1 and Muhammad Dawood2
...onfirmed the presence of genetic variability among Lohi animals; this variation might be associated with other economic traits for future breeding programs and marker assisted selection.
...
Sana Zahra
... of Pakistan. A detailed genetic evaluation of four unrelated but ethnically similar families from Punjab province was performed.Linkage to SLC26A4 locus was confirmed through linkage analysis using Short Tandem Repeat Markers (D7S2420, D7S2459 and D7S2456). p.Y556C was identified as causative mutation by Sanger Sequencing. A known missense mutation p.Y556C in exon 15 of SLC26A4 gene was identified in all the four families. It may be concluded th...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
...assaemia is an inherited genetic disorder of haemoglobin. It is estimated that about 100,000 patients are presently suffering from thalassaemia major, the severe form of the disorder. The patients of β-thalassaemia are dependent upon lifelong blood transfusion. Multiple transfusions expose them to many blood borne diseases, most commonly hepatitis B and C. The aim of current study was to determine the prevalence of HBV and HCV infections among thalassaemi...
Ehtisham Shakeel Khokhar*1, Amir Shakeel1, Muhammad Amir Maqbool1, Muhammad Khubaib Abuzar2, Sumaira Zareen2, Syeda Sana Aamir4 and Muhammad Asadullah4
...dicating the presence of genetic variability except fiber fineness. δ2GCA/δ2SCA ratio depicted the predominance of non-additive types of gene action for plant height, number of monopodial branches, number of sympodial branches, numbers of bolls, boll weight, seed cotton yield, lint percentage, fiber length and fiber strength. The parents VH-259, CRS-456 and AA-703 among lines and PB-39 among testers displayed significant GCA e...
Ambreen Asghar, Tasleem Akhtar, Nadeem Sheikh*
...and obesity. Despite the genetic regulation of metabolic responses, a close relationship between high energy food-intake and increased body-weight has been reported to support several pathologies in the metabolic and immune system. Present work was designed to evaluate the variations in serum protein level induced by fat rich diet. For this purpose, two groups of Wister rats were fed with diets carrying difference in percentage of fats. Protein profiling of tr...
Ligia Neves Scuarcialupi, Laila Andreia Rodrigues Beserra, Julia Rosas Hochheim, Rodrigo Martins Soares, Fabio Gregori*
...ere further submitted to genetic sequencing and analyzed by phylogenetic analysis. There were 5 RVF positive samples (5.15%) in 2 broilers, 1 laying hen and 2 breeders. Only one RVG sample (1.03%) was detected in laying hen, in coinfection with RVF. This data contributes to a better understanding of rotavirus distribution in Brazil.
...

Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*

...sified into two distinct genetic subclades (2.2.1.1 and 2.2.1.2) in Egypt. This study was conducted as part of routine surveillance activities in Egypt; 5202 cloacal and oropharyngeal swabs were collected from live/dead birds, including commercial and backyard flocks, in five different locations in Egypt between the years of 2015 and 2016. All samples were screened using virus isolation, as well as antigenic and molecular detection methods. Full-length hemaggl...
Shuai Shang1,2, Honghai Zhang2,*, Xiaoyang Wu2, Qinguo Wei2, Jun Chen1, Huanxin Zhang1, Huaming Zhong2 and Xuexi Tang1,*
...n, we constructed a phylogenetic tree using Tas2r genes sequences from these fifteen species. Phylogenetic analyses showed that most Tas2r genes of raccoon dogs were closed to other canid-species, indicating that the Tas2r genes were very conservative. Besides, we also detected positive selection in 11 intact genes in raccoon dogs. The results of the positive selection analyses indicated that Tas2r10<...

Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1 

... there were considerable genetic differences among the Trichoderma isolates. While, there was no correlation, found between RAPD clusters and aggressive groups (AGs). Isolates belonging to different AGs were found scattered among the four RAPD clusters. It was concluded that the indigenous isolates of Trichoderma harzianum were found effective against root knot nematodes and there was a significant difference among these isolates, at the molecular level. ...
Aqeela Ashraf1,2,*, Muhammad Imran1 and Yung-Fu Chang2
...industry due to its high genetic variability, enormous number of environmental sources and increased resistance to antibiotics. The present study was aimed to determine the antimicrobial resistance of E. coli isolates from bovine mastitis and identification of antimicrobial resistance and virulence genes associated with them. Antimicrobial susceptibility testing was performed by disk diffusion method. Resistance and virulence genes were detected by PCR....
Baoying Guo*1, Yu Chen1, Chuan Zhang2, Zhenming Lv1, Kaida Xu3, Hongling Ping4 and Huilai Shi4
...es, and constructed phylogenetic trees estimating their relationships. The genome was 16,228 bp and contained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 2 long-noncoding regions [both in the control regions (CR)]. The composition and order of genes in S. lycidas were similar to those of most other invertebrates. The overall base composition of S. lycidas is 35.8% T, 14.8% C, 41.3% A, and 8.1% G, with extremely high...
Zeqin Fu, Yunfang Tian, Yingying Ye*, Pengzhi Qi and Changwen Wu
...o choose for researching genetic diversity and population structure and provide more in-depth understanding of Mytilus coruscus.
...
Jun Chen1, Shuai Shang2, Xiaoyang Wu1, Jiakuo Yan1, Huaming Zhong1, Huanxin Zhang2, Weilai Sha1, Wanchao Zhu1 and Honghai Zhang1,*
...k offers an initial phylogenetic baseline for further research on the intestinal ecosystem of these African animals. This work is of great significance for disease monitoring and protection of these animals.
...

Fatma Abdallah1*, Ola Hassnain2, Elsayed Attar3, Haytham Ali3,5, Mohamed Megahed1 and Venugopal Nair

...d for alignment and phylogenetic analysis. The eight studied consensus amino acid sequences of Meq proteins possess several amino acid mutations associated with the MDV virulence and a unique distortion in the Proline repeats (Proline-to-Alanine) at position 176 in the Egyptian MDV strains. The Phylogenetic analysis grouped the eight analysed sequences with the previously investigated Meq from Egypt (2011-2013) together with...
Adel A. Basyouny Shahin,1,* Amira M. Metwally Mohamed2 and Abd El-Reheem A. El Shatter2

 

...xamined at 17 structural genetic loci and interrelationship was discussed. Of these 17 genetic loci, 10 (58.8%) loci were monomorphic with the same allele fixed in all individuals from both karyotype forms and only 7 (41.2%) loci were polymorphic with two different alleles. Levels of genetic variability between the two forms were relatively low and are comparatively either within the range...
Zihong Chen1, Ling Xu2, Xiaona Yang2, Yaguan Zhang3* and Yuming Yang4
...ed with a multigene phylogenetics analysis involving 5.8S–ITS, nrSSU, nrLSU, EF–1α, RPB1 and RPB2. In multilocus phylogeny, M. baoshanense was grouped as a sister clade to Metarhizium majus, Metarhizium guizhouense, Metarhizium lepidiotae and Metarhizium indigoticum, but conclusively separated from these allied species with high support value. Morphological character differentiated M. baoshanense from...
Selçuk Kaplan
...ry important to increase genetic gains in breeding. Leptin is a 16 kDa protein which is highly expressed in adipose tissue. Leptin is one of the most significant candidate gene marker for MAS studies. Therefore, bubaline leptin gene exon 2, part of the intron 1 and intron 2 region were amplified and sequenced to identify nucleotide variations in Anatolian water buffaloes. Sequence analysis were revealed seven polymorphic site (G1072A, T1081C, T1131G, T1143C, T...
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
...argely influenced by the genetic background of cattle. Therefore, we analyzed the transcriptome changes within adipose tissue in beef cattle at different stages of growth. Microarray analysis of data at 12 vs 18 months, 12 vs 24 months, 12 vs 30 months, 18 vs 24 months, 18 vs 30 months and 24 vs 30 months uncovered a total of 10625 differentially expressed genes (P ≤ 0.05; no fold change). The dynamic impact approach (DIA) bioinformatics tool was used to un...
Muhammad Shahid Saeed1, Adeela Shahid2, Samia Jawed3, Muhammad Akram4, Irshad Hussain Qureshi5 
...plex interaction between genetic, behavioral and environmental factors. There are many drugs available for asthma and magnesium sulphate is given intravenously as an important adjunct therapy during the acute severe attack of asthma
Objective: This study was panned to determine the effect of intravenous MgSO4 on pulmonary function test in acute severe attack of asthma.
Methodology: A double blind placebo control...

Ghulam Abbas1, Muhammad Jawad Asghar1, Muhammad Rizwan2*, Muhammad Akram1, Jaffar Hussain1 and Fiaz Ahmad1 

... requires information on genetic variability and correlation of traits. For this purpose, fifty eight exotic and indigenous diverse mungbean genotypes were evaluated for seed yield and other related traits. The genetic analysis of mungbean germplasm revealed high genotypic (GCV) and phenotypic (PCV) coefficients of variability for biological yield (GCV% = 31.70, PCV% = 33.58), harvest index (GCV% = 27.80, PCV% = 30.16) and s...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
...ter understanding of the genetic properties of the FAdV strains responsible for recent outbreaks, the sequence analysis of hexon gene of 10 isolates associated with IBH and HPS was determined. Sequence alignment and phylogenetic analysis based on direct sequencing of hypervariable region of hexon gene grouped these sequences into two distinct groups, three FAdV isolates were clustered together belonging to fowl adenoviruses ...

Rizwana Qamar1, Maria Ghias1, Fida Hussain1, Sajida Habib1, Muhammad Khuram Razzaq2*, Muhammad Aslam1 and Imran Habib3 

... presence of significant genetic variability among hybrids and inbred lines. Hybrids had more tolerance to drought conditions on both levels of water stress treatments. With the increase in stress, root length and leaf temperature increased, while shoot length, plant fresh and dry weight, chlorophyll fluorescence and relative water content decreased at seedling stage. Hybrid FH-630 and Hysun-33 are identified as the most drought tolerant genotypes used in pres...

Imadud Din1*, Fazal Munsif1, Irfan Ahmad Shah3, Hamayoon Khan1, Fahad Ullah Khan1, Ibrarullah2, Shahab Uddin4 and Tauqir Islam1 

...riation (PCV), range and genetic advance were estimated for yield and yield associated traits. Results revealed significant variation for all traits among the tested wheat genotypes. The results showed the existence of adequate genetic variability among the tested wheat genotypes. High PCV and GCV were found in grains spike-1, while highest broad sense heritability with inferior genetic ad...
Maryam Javed*, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem and Wasim Shehzad
...l behavior having strong genetic relation. Current study focused mainly on the screening of genetic marker V158M located in COMT gene for its relation to physical aggression in the four domestic bovine species of Pakistan, which are normally less aggressive. Selected animal species were cattle (Bos indicus), buffalo (Bubalus bubalis), sheep (Ovis aries) and goat (Capra hircus). Selected marker V15...

Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla

...100%, respectively. Phylogenetic analysis revealed that Hail PPRV strains clustered in four separate groups within lineage IV indicating genetic divergence based on N gene. These findings further support the continuous characterization of PPRV strains in small ruminants to support the global eradication plan in 2030. 

...
Tanveer Hussain1,*, Masroor Ellahi Babar1, Marcos De Donato2, Abdul Wajid1, Asif Nadeem3, Zahoor Ahmad3, Waqas Ahmad Khan4, Sunday O. Peters5 and Ikhide G. Imumorin6
...nderstand the origin and genetic diversity of native cattle breeds (Bos indicus) in Pakistan, partial sequencing of the mitochondrial gene cytochrome b (339 bp) was done in 136 individuals from 10 different breeds. Analysis of the Cyt b gene showed high conservation in Pakistani cattle as expected, with only 26 individuals showing nucleotide changes. Only 5 point mutations were present in multiple individuals (SNP), but one was specific for indicine cat...
Wang-Dui Basang1, Tian-Wu An2, Luo-Bu Danjiu3, Yan-Bin Zhu1, Shi-Cheng He3, Xiao-Lin Luo2, Wei-Wei Ni4, Xiao Wang4, Shu-Zhu Cheng4, Jian Wang4 and Guang-Xin E4,*
....00894) population. Phylogenetic analysis revealed that these four populations separated into two haplogroups; the first included SN, DX, and NR, and the second included JL. However, no significant divergence was found among the ecotype populations using a pair-wise difference comparison (FST). Thus, the Naqu yak has multiple maternal origins and demonstrates high diversity within 4 geographic ecotype yak populations. In addition, the yaks ex...
Riaz Aziz Minhas1,*, Muhammad Nasim Khan1, Muhammad Siddique Awan1, Basharat Ahmad1, Syda Shaista Bibi1, Mohsin Hanif1 and Afsar Mian2
...might have experienced a genetic isolation or inbreeding. We assessed the level of genetic diversity and genetic isolation of different langur populations using Randomly Amplified Polymorphic DNA (RAPD) genetic markers. We collected 86 different samples (feces 64, hair 13, blood 5, tissues 4) from 5 geographic langur populations of Pakistan and Azad Jamm...
Jingen Xu1, Yang Liu2, Erhui Jin1, Youfang Gu1, Qin Zhang3 and Shenghe Li1,*
... of immune functions and genetic control of peripheral blood T lymphocyte subsets. Also, several differentially expressed immune-related genes may be candidate genes for disease resistance breeding in pigs.
...
Wei Meng1,3, Tianyan Yang2,*, Yunguo Liu1, Mahmut Halik1 and Tianxiang Gao2
...equenced. Besides, their genetic characteristics were also identified and compared simultaneously. Genetic distance and sequence differentiation suggested that great genetic variation existed within species and the sample from Kaidu River in South Xinjiang might be a cryptic species or subspecies of G. dybowskii. The phylogenetic analyses from 12 ...
Ai Guo1,2,3, Jiaguang Xiao4, Binbin Shan4, Tianxiang Gao5 and Yongdong Zhou3,*
...To better understand the genetic diversity and phylogeny of Coilia, the complete mitochondrial genomes of two lineages in Coilia mystus were compared. They were all typical circular double stranded DNA molecule with 17075 bp in C. mystus N and 16964 bp in C. mystus S, respectively, containing the standard metazoan set of 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and non-coding regions. The mitogenomes of ...

Muhammad Amir Maqbool1*, Muhammad Aslam1, Waseem Akbar2, Muhammad Waheed Anwar3 and Ehtisham Shakeel Khokhar

...stalX software, and phylogenetic trees were constructed by using MEGA5 software. Medicago truncatula found to have total 35 MIPs which further comprised of 10 MtPIPs, 6 MtTIPs, 17 MtNIPs and 2 MtSIPs. Cicer arietinum genome comprised of 41 MIPs which were consisted of 10 CaPIPs, 12 CaTIPs, 15 CaNIPs and 4 CaSIPs protein sequences. Genome of Zea mays was carrying 36 ZmMIPs which were categorized into 12 ZmPIPs, 13 ZmTIPs, 8 ZmNIPs and 3 ZmSIPs. Phylo
Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3,*
...don usage analyzed. Phylogenetic Bayesian and ML analyses were conducted, using a concatenated set of 12 protein-coding genes, and adding 16 other species of gobies (Gobiidae). The mitogenome sequences of O. lacepedii, O. rebecca and Odontamblyopus sp. were all circular double-strand molecules, 17245 bp, 17009 bp and 17004 bp long, respectively. Compared with other bony fishes, the three species shared the similar features in gene arrangem...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
...uence alignment and phylogenetic analysis were performed to compare SlNPV- FSD15 based on Lef-8 with other Lef-8genes sequences clearly showed that our SlNPV-FSD15 isolate belongs to Spodoptera litura associated NPVs. The biological activities of this NPV isolates were investigated under laboratory condition. The highest mortality of S. litura was observed at early instars. Against second instars of S. litura, LC5...
Naeem Ahmad1,* and Zulqurnain2*
... that GIFT-Th has better genetic factors for weight gain as compared to GIFT-Tw, and should be preferred for farming, however an economic analysis for both strains determining benefit to cost ratio is pre-requisite before final recommendations.
...

Yasir Ali1*, Muhammad Aslam Khan1, Muhammad Atiq1 and Muhammad Hussain2

...gist, plant breeders and genetics to develop non-specific rust resistant wheat varieties through gene pyramiding or marker assisted breeding 

...

Fan Shuli1*, Ameer Hussain Jarwar1,3, Xiaoyan Wang2, Long Wang1 and Qifeng Ma

...ion/tons. Generally, the genetically modified cotton (Bacillus thuringiensis) was acquaint in Pakistan during the first decade of this century to moderate harmful effects of lepidopterous insects; which were causing major loss to the cotton production ultimately to the economy of the country. Currently the genetic modified cotton cultivation consumed for more than 95% area under cotton cultivation. There are certain pros and...

Arshad Iqbal1*, Syed Mehar Ali Shah1, Hidayat ur Rahman1, Faiza Aman2 and Aziz-ur-Rahman

...bility, heritability and genetic advance among F5:6 lines of rice for various morphological traits. Thirty-eight lines derived from F5 rice cross combinations along with their 8 parents were planted in a randomized complete block design (RCB) having three replications at The University of Agriculture, Peshawar during 2016 rice crop growing season. Data were taken on some of the morphological traits like number of days to heading (days), number of days to matur...
Yuan Li1, Liyan Zhang2, Karhoe Loh3, Ji Feng1, Xinqing Zheng1, Puqing Song1 and Longshan Lin1,*
...tudies on the population genetics of P. minor are not yet available. In the present study, the mitochondrial Cytb gene was employed to investigate the population genetics of P. minor collected along the coasts of China and Malaysia. The genetic diversity of all P. minor populations was moderate, and two major haplotype lineages were detected that were differentiated ap...

Sher Nawab Khan* and Ghulam Hassan 

...ant (b) and additive (a) genetic effects were detected for all the parameters except spikelets spike-1. Scaling test for the adequacy of the model was complete to partially adequate for the studied parameters. Similarly, both dominant (H) and additive (D) genetic components were observed significant for the studied characters. Moderate to high broad and narrow sense heritability was recorded for the studied traits which indi...

Waqas Ali* and Mudasser Habib 

...e vaccinal strains. Phylogenetic analysis (maximum likelihood) showed that lineages Pan Asia II, An Iran-05, and Group VII within serotype O, A and Asia 1 are causing majority of the outbreaks in the Pakistan during recent years. Moreover, only one residue of VP1 region was under positive selection pressure for serotype A (position 141) located in known critical antigenic region but as a whole protein was under negative selection pressure for serotype O, A and...
Shagufta Naz*, Saima Sharif, Hafsa Badar, Syeda Fareeha Tauheed
...y diagnosis, genotyping, genetic counseling and proper treatment (corneal grafting) are necessary for the restoration of optimal vision and should be done to avoid the severe outcomes caused by MCD.
...
Fehmeeda Fatima, Asif Nadeem* and Maryam Javed
...enced (1344 bp) to study genetic diversity and evolutionary characteristics of Sindh ibex. In this research, samples of Sindh ibex were collected from Kirthar National Park, Sindh, Pakistan, the gene of interest was amplified, PCR products were sequenced and data was analyzed. The results of genetic diversity indicated that Parsimony informative sites were found to be 11 while Singleton variable sites were not detected. The ...

Muhammad Arshad*, Sabeeta Jan, Sundas Awan, Samra Azam, Shiguftah Khalid and Muahmmad Ayub Khan 

...ronomic traits to assess genetic specifications, genetic divergence, and correlation coefficient, direct and indirect effects of eight characters towards seed yield under field condition. Experiment was conducted according to RCBD design at NARC (National Agricultural Research Centre), Islamabad during year 2015. Data were recorded on Days to flower initiation, Days to flower completion and Days to maturity, 100 seed weight,...

Nasir Raza Zaidi1, Mian Waheed Ahmad1, Mahesh Gautam1, Riffat Mehboob2*

Risk factors for multiple sclerosis in Pakistani population- A crosssectional study
.... Many environmental and genetic factors have been associated with MS. The aim of this study
is to identify the possible risk factors that are linked to MS in Pakistani population. This study was conducted in
Department of Radiology, Mayo Hospital Lahore from 2013 to 2014. The first hundred patients who came for
reporting MRI of the brain and had positive findings of MS were retrospectively evaluated. Mean age of the patients
was 43...

Sadaf Niaz1, Masroor Ellahi Babar2, Tanveer Hussain2, Asif Nadeem1, Misbah Hussain1, Riffat Mehboob3*, Fridoon Jawad Ahmad3

Mutation analysis of RING1 domain of Parkin in early onset of Parkinson’s disease in Pakistani patients-a pilot study
...he data suggests that no genetic predisposition in RING1 domain may be responsible for the occurrence of
disease in local population. It may be due to genetic changes in any other part of the gene or due to other
environmental factors. However, we have limitation of sample size due to less occurrence of disease in this area. The
current study is very important for successive researches in Pakistan as the...

 Halil Erhan Eroğlu

The comparison of the Felidae species with karyotype symmetry/asymmetry index (S/AI)
... the S/AI values, a phylogenetic tree was drawn showing
relationships among the species.

...
Aiguo Zhou1,3,4, Shaolin Xie1,4, Zhenlu Wang1, Lanfen Fan1, Yanfeng Chen2, QiaoYe1, Fang Zeng1 and Jixing Zou1,*

 

...ze the relationships and genetic diversity of two color morphs of northern snakehead. For the Chnannidae family, the average genetic distances of Cyt b gene was 0.171 (ranged 0.000 to 0.248) with the inter-species genetic distances. The intra-species genetic distance ranged from 0.000 to 0.002. For the haplotypes of two color morphs of Channa a...
Faiz Muhammad1,2, Canfeng Dou1, Zhen-ming Lü1,*, Li Gong1, Xun Du1 and Muhammad Shafi3
...he neighbor-joining phylogenetic analysis revealed three lineages. The Dongshan population distinctly has shown separate lineage. The haplotype networking has shown that Hap1 contributed 48.051% and appeared in six populations whereas, Hap6, Hap11, Hap 16 and Hap17 were also shared by some populations, rest of the haplotypes were unique. This study solely provide species specific information on its genetic structure and popu...
Jun Yan Bai1,*, Yong Gang Zhao2 and Yu Qin Wang1
...s laying foundations for genetic marker of growth traits of goats. In this study, GHRL genes of black goat and Yaoshan goat were collected for PCR amplification. Polymorphism of goat species was tested by sequencing technology. Results demonstrated that there are two mutation sites (SNP loci) of the primer GHRL-2 in black goat and Yaoshan goat, which are G447C site and T498C site. At G447C site, gene frequencies of G and C in black goat and Yaoshan goat were d...

Mubashir Ahmad Khan*, Nazakat Nawaz, Muhammad Arshad, Muhammad Ayub Khan, Tahira, Doulat Baig, Ihsan Ullah Khan and Shamim ul Sibtain Shah 

...here was a wide range of genetic diversity in this set of sesame germplasm which could be utilized for crop improvement. It is further recommended that for making improvement in sesame crop, identified superior sesame lines viz. SG-115, SG-103, SG-78, SG-79, SG-31, SG-117, SG-108, SG-41 and SG-1 could be used in breeding program and further by making selections in segregating generations. This will help to increase sesame crop yield in the country. 

...

Muhamad Jawad*, Shahid Riaz Malik, Muhammad Aqeel Sarwar, Muhammad Asadullah, Israr Hussain and Rabia Khalid 

...onducted to evaluate the genetic diversity among 36 lentil genotypes for mechanized harvesting. Analysis of variance for qualitative and quantitative traits showed significant variability among the genotypes. Correlation analysis indicates negative correlation of yield with lowest pod height and highly significant correlation between lowest pod height and plant height. Cluster analysis performed for qualitative and quantitative traits separately. It was observ...

Muhammad Ilyas*, Sardar Ali Khan, Shahid Iqbal Awan and Shafiq-Ur-Rehman 

...s. Both heritability and genetic advance estimates were higher for grain yield and plant height, hence additive gene action was depicted. While, narrow sense heritability and genetic advance was lower for remaining parameters under both the environmental conditions which clearly specified that non-additive gene action was present. Results indicated that the said breeding material might be better suited for heterosis breeding...

Ali Awais, Charassri Nualsri and Watcharin Soonsuwon* 

...justify;">To enhance the genetic variability and to create new and diverse characters to a natural population, researchers sometimes opt for mutation breeding. This research has been done to investigate the effective doses of EMS and to observe the phenotypic variability of the two upland rice genotypes Dawk Pa-yawm (white rice) and Dawk Kha 50 (red rice), in Thailand. Seeds of the potential genotypes has been treated with varying EMS concentrations. With the ...
Zhiwen Zou, Jianfei Xi, Fen Chen, Rui Xu, Tianrong Xin and Bin Xia*
...om GenBank. And the phylogenetic relationship between the Amblyseiinae genus and the subspecies was studied. The results showed that four species of Kampimodromus and two species of Euseius clustered into two different branches, but the branch of 8 species of Amblyseius and Neoseiulus was chaotic. It indicated that the species of Neoseiulus and Amblyseius should be assigned carefully. The results could not only reveal ...
Juan Augusto Hernández-Rivera1, Jaime Molina-Ochoa2,*, Luis Jorge García-Márquez1, Omar Francisco Prado-Rebolledo1, Rafael Julio Macedo-Barragán1, Arturo César García-Casillas1 and Muhammad Irfan Ullah3
...mplate other traits like genetic potential, lactation (305 d), lactation number, fertility rate, and economic impact to observe clarity the potential of each crossbred in dairy cattle in the future.
...
Jam Nazeer Ahmad1,2,*, Muhammad Jafir1, Muhammad Wajid Javed1, Sumaira Maqsood3 and Samina J.N. Ahmad1,2,*
...n fields in Punjab. Phylogenetic analysis was also complemented to differentiate DCB identified from other countries of the world. The PCR bands obtained in gel electrophoresis amplified PCR fragments from all DCB species at 710 bp. The sequencing results and phylogenetic analysis with sequences submitted at NCBI database revealed that DCB have 99-100% similarity with Oxycarenus hyalinipennis OH1 (JQ342987.1), Oxyc...

Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1 

...c strains. However, phylogenetic analysis revealed clustering of all tested strains within sub-genotype VIg. Three isolates were assessed for pathogenicity based upon the mean death time (MDT), the intracerebral pathogenicity index (ICPI) and the intravenous pathogenicity index (IVPI) in chicken. The ICPI and MDT revealed that all tested isolates were of moderate virulence (Mesogenic) in chickens. Mature chickens showed no clinical signs or death as assessed b...
Ajmal Khan Kassi1,*, Humayun Javed1 and Tariq Mukhtar2
...ochemical, molecular and genetic characteristics of the plants. As the information about the relationship between physico-morphic characteristics of commercially grown okra cultivars and resistance or susceptibility to Helicoverpa armigera is lacking, therefore, in the present study relationship between physico-morphic characteristics and resistance or susceptibility of okra cultivars to H. armigera was evaluated. The heights and stem girths of c...
Rishen Liang*, Meng Zhou, Zhenxiang Lin, Guozhang Li, Yuan Chen, Xuan Lin and Zaohe Wu
...%. In the molecular phylogenetic trees, the longer branch length also clearly distinguished the independent placement of the two species. These results revealed great genetic differences between P. orientalis and P. vittatus, and strongly suggested that they might be two distinct species and should not be placed as synonym.
...
Liyan Zhang1,2, Zhidong Zhou1,3, Haiping Li1,2, Yanlong Qiao4, 5 and Yueping Zhang1,3,*
... evaluate the population genetics and elaborate the historical demography of Scomber japonicus collected from four geographical locations in the Taiwan Strait and its adjacent waters. The results of genetic diversity and genetic structure analysis of S. japonicus showed that these four populations exhibited moderate genetic diversity across...
Zhen-Yang Wu1, Guang-Xin E2,*, Hui Ran1, Da-Hui Wang1 and Tian-You Yang1
...00000) populations. Phylogenetic analysis revealed that these 10 populations could be separated into two clusters: the first included CD, WS, YP, JK, and ST, and the second included DJ, YJ, YH, SQ, and SN. However, there was no significant divergence between populations based on pair-wise differences (FST). Overall, the 10 populations were divided into two subgroups and two D-Loop haplotype clusters. This study showed that the different rice ...

Syed Atif Hasan Naqvi1*, Sidra Mushtaq1, Muhammad Tariq Malik2, Ummad-ud-Din Umar1, Ateeq ur Rehman1, Shoaib Fareed3, Muhammad Asif Zulfiqar

...y selecting the improved genetic material. Because it will have a good impact on product quality, economic output and its potential against insect pest or disease. 

...

Khadim Hussain Wagan1*, Muhammad Ibrahim Khaskheli2, Jamal-U-Ddin Hajano1 and Abdul Ghani Lanjar2 

...hrough sequence and phylogenetic analysis. There was significant variation (Df= 54, F=7.4, P=0.0000) among cfu per gram of M. phaseolina in soil samples collected during field survey at different districts of Sindh during 2015 and 2016 growing year. In soil sample maximum 41.371 cfu per gram of M. phaseolina with range of 27.00-48.40 were counted from Badin during 2016 followed by 40.2 with range of 27.00-45.20 during 2015 growing season. The plant tissues wer...

Manzoor Hussain1, Muhammad Awais2, Farhatullah3, Saiqa Bibi1, Muhammad Rameez Khan3, Afifa Khalid1 and Sajid Ali3* 

...heir exploitation in oat genetic improvement and crown rust control. 

...
Iram Maqsood1,2,3 and Ke Rong *1,2
...racteristics, population genetics, and organ preservation. In addition to this, the habitat restoration program committed to improving the quality and habitat area. The reintroduction project illustrated that A. Sinensis bred in captivity well adapted to wild habitat and successfully reproducing their offspring’s. Only healthy crocodiles were selected for release into the wild to renew natural populations. Chinese alligator currently under strict ...

 Tazeen Jamil, Saba Ijaz, Rabia Arif*, Faiza Akram and Muhammad Saleem

... an inevitable source of genetic diversity due to their abundance in eukaryotic genomes and their insertion in coding regions leads to altered gene expression. We examined the diversity of retroelements in four consecutive generations of different strains of Sordaria fimicola. The parental strains werecollected from two contrasting environments of Evolution Canyon i.e. harsh South facing slope and neutral North facing slope. Retroelements profile...
Faiz-ul Hassan1,*, Muhammad Sajjad Khan1, Muhammad Saif-ur-Rehman1, Syed Muhammad Raihan Dilshad2 and Muhammad Amjad Ali3
...ands was performed. Phylogenetic tree was constructed by UPGMA method to determine the genetic similarity among the horse breeds. Thoroughbred and Arab horse breeds showed a higher similarity with Pakistani indigenous breeds, suggesting that these two exotic breeds had contributed significantly in the development of indigenous horse breeds of Pakistan. One set of primers OPL-7 revealed a polymorphic band at 450 bp which was ...

Ihsan Ullah Khan*, Muhammad Arshad, Muhammad Ayub Khan, Muhammad Ashraf, Ashiq Saleem, Sundas Awan, Samra Azam and Shamim Ul Sibtain Shah 

...breeding in sunflower if genetically more diverse germplasm is incorporated in breeding programs. 

...
Bibi Nazia Murtaza1,2, Azhar Qayum3, Shamaila Inayat Nadeem1, Naif Awdh Al-Maliki4, Abdulaziz Alamri4 and Abdul Rauf Shakoori2,5,*
...th, RasGTPases behave as genetic switches. This transient process of GAP-mediated GTP hydrolysis becomes altered when the Kras gene is mutated. The most common Kras mutations are found in codon 12 and 13 and 61. Some other noncanonical mutations have been reported in codon 11, 14, 15, 17, 18, 19, 20, 22, 27, 30, 31, 117, 146 and 154. We aimed to demonstrate the conformational changes induced in two novel K RAS variants, p.E31K and p.G138V, identified in two CR...
Faiza Jabeen1,*, Bushra Muneer2 and Javed Iqbal Qazi3
...gelatin and starch. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates belong to the same specie Anoxybacillus rupiensis with 99% similarity under Accession number KF254911 (JF82) and KC849452 (JF83). The DNA G + C contents were 56.854% and 56.754% for A. rupiensis JF82 and A. rupiensis JF83, respectively. The isolates grew over a wide range of temperatures (40-70°C) and pH (4-9). The isolates exhibited ...
Zhaochao Deng1, Jingchen Chen1, Na Song2, Yongzhen Li3 and Zhiqiang Han1,*
... distribution and marked genetic structure. However, analysis of this structure has not included the South China Sea population. In the present study, we collected a total of 23 individuals from Xisha, and Nansha Islands in the South China Sea and a 503 bp segment of mitochondrial DNA control region was sequenced. 17 polymorphic sites were obtained defining 7 haplotypes in the South China Sea population. Contrary to heterogeneous expectation, AMOVA and pairwis...
Zhaochao Deng1, Na Song2, Yongzhen Li3, Tianxiang Gao1 and Zhiqiang Han1,*
...China Sea. To reveal the genetic structure and genetic diversity of L. olivaceus, a 495 bp segment of mitochondrial DNA control region was sequenced. We collected a total of 65 individuals from three archipelagos (Xisha, Zhongsha and Nansha) in the South China Sea and 107 polymorphic sites were obtained defining 39 haplotypes. Based on the NJ tree, two distinct lineages were detected, with strong frequency differences...
SiRui Wang1,2, Fekede Regasa Joka1,2, XiaoLong Wang1,2,* and SuYing Bai2,*
...selection pressure. Phylogenetic analyses of the reported sequences of jungle fowl (Gallus gallus) and the 10 tested birds were performed. A total of 10 nucleotide sequences of the GED region of the Mx gene in wild birds were obtained, with a length of 231 bp, encoding 77 amino acids. The third locus in all tested amino acid sequences contains a Ser residue that corresponds to amino acid position 631, the virus resistance site, in the chicken ...

Qaizar Ahmed1, Fida Mohammad2, Sheraz Ahmed2*, Sultan Akbar Jadoon2, Imtiaz Ali2 and Ajmalud Din2 

...cise knowledge regarding genetic architecture of production traits is important to devise effective breeding and selection strategies. This investigation was carried out to elucidate relationship between Hayman and Griffing analyses in flue cured Virginia (FCV) tobacco. Plant material comprised of seven tobacco cultivars which were hybridized in full diallel mating system at the Tobacco Research Sub-station, Mansehra, Pakistan during 2007. Thereon, seven paren...
Fukuan Du1,2, Yongkai Tang1, Juhua Yu1, Shengyan Su1, Fan Yu1, Jianlin Li1, Hongxia Li1, Meiyao Wang1 and Pao Xu1,2,*
...s very urgent to perform genetic research on C. nasus to protect the wild population. In this study, we sequenced the brain transcriptome of C. nasus using Illumina Hiseq 4000 platform. We obtained 123,764 unigenes with an average length of 1092 bp and a total of 115,169 putative Simple Sequence Repeats (SSRs). Among them, 37 SSRs were selected for the validation experiments. All of the loci were found to be polymorphic and showed bi-allelic in 6...

Aziz Ur Rahman* and Syed Mehar Ali Shah 

...ated traits in rice. The genetic material comprised 87 rice F5:7 recombinant inbred lines (RILs) along with three check cultivars namely Pakhal, Kashmir Basmati and Fakhr-e-Malakand. The genetic material was planted in alpha lattice design using three replications across different locations namely Peshawar, Swat, Manshera and Charsadda of the Khyber Pakhtunkhwa province of Pakistan during 2017. Pooled analysis of variance in...
Shengjie Zhou1,2, Pengfei Wang1,2, Chao Zhao1,2, Mingjun Fu3, Jian G. Qin4, Lihua Qiu1, Zhenhua Ma1, 4,* and Maoshang Lin1
...FABP gene in the ontogenetic development of golden pompano.
...

Khan Sher1*, Muhammad Nisar2 and Muhammad Subhan1 

... of dissimilarity in the genetic makeup ranging from 0.91 to 0.11. All the landraces of common bean collected from Malakand divisions KP, Pakistan, showed significant variations. 

...

Abid Ali1*, Hidayat ur Rahman1, Farhatullah1 and Zahir Shah

Hayat Zada1, Ahmad-Ur-Rahman Saljoqi1,*, Ijaz Ali2, Bashir Ahmad1, Abdul Waheed Khan2 and Shabeer Ahmad2
...mean gene frequency (f), genetic distance (I) and Shannon’s information index (h) for three populations were 1.336, 0.304 and 0.437, respectively. Nei’s unbiased measures of genetic identity and genetic distance revealed that higher genetic distance was observed among the isolates from Kalam and Madyan (97.87 %) whereas low
Syeda Ain-ul-Batool1, Sadia1, Kathrin Blasius2,3,4, Angela Kaindl2,3,4 and Ghazanfar Ali1,*
...al recessive ciliopathic genetic disorder in humans. It is a multisystem disorder and is principally described by visual abnormalities, con-rod dystrophy, eyes exotropia, obesity, polydactyly, hypogonadism, and renal abnormalities. Few additional features of BBS also include delayed motor development, clumsiness, anosomia, ataxia, hypodontia, hearing impairment, hirschsprung disease, cardiovascular and liver disorders. So far 21 genes are reported that cause B...
Asma Yousafzai1,*, Muhammad Luqman2, Nisar Ahmed1, Muneeza Arbab1,Muhammad Murad1, Naheed Sajjad3, Sara Naudhani1, Shakeela Daud1, Abdul Hameed Baloch4, Khushnaseeb5, Sana Zahoor6, Zahid Rauf7, Muhammad Mohsin Javed6 and Jamil Ahmad1
...s were used to determine genetic variants in BRCA1 and BRCA2. Nine variants were identified in BRCA1 and four were identified in BRCA2. In case of BRCA1, six missense substitutions (p.Asp343Tyr, p.Gly393Asp, p.Ser561Phe, p.Ser616Phe, p.Pro871Leu and p.Ser1613Gly) two frameshift (p.Ser423fs and p.Gly1770fs) and nonsense mutation (p.Glu1250X) was identified. In case of gene BRCA2, all the four variants were missense subs...
Songhao Zhang1, Yiwen Gong1, Jian Xu1, Mou Hu2, Peng Xu3 and Yanliang Jiang1,2,4,*
... However, the underlying genetic basis is less studied. In the present study, we utilized the powerful approach of comparative transcriptome analysis via high throughput sequencing, and examined transcriptome profiles of purse red carp by comparing with Yellow River carp, of which the skin color and body shape are quite similar to wild carp. A total of 434 million reads was generated, resulted in 203,590 assembled contigs, which was used as the reference seque...
Zhengfei Wang*, Dan Tang, Xuejia Shi, Huayun Guo, Xiuping Chen, Daizheng Zhang and Boping Tang*
... ecosystem. However, the genetic basis for the adaptation of hydrothermal vents crabs to the harsh environment remains poorly explored.The objectives of this study were to increase our understanding of the mechanisms of hypoxia adaptability in vent crabs and to confirm if there is a correlation between mitochondrial protein coding genes (PCGs) and adaptation to the extreme hydrothermal vent environment.Thirteen PCGs from mitochondrial genomes of 48 Brachyura s...
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*

 

...nd can be modified by epigenetics. However, whether HSPC117 affects development of cloned embryos mRNA expression is unknown. To investigate the influences of HSPC117 on embryonic development, we generated transgenic porcine embryos by handmade cloning. We then assessed the embryonic developmental rate at cleavage and blastocyst stages. Our results showed that the HSPC117 transgenic embryos had markedly higher cleavage and blastocyst rates...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
...media of porcine parthenogenetic embryos experienced different types of microopetation using Raman spectroscopy. The results showed that Group II with the puncture of egg zona and plasma membrane had the highest cleavage rate and blastocyst rate of porcine parthenogenetic embryos, which was significantly higher than Group I with intact oocytes (P<0.05) and Group III with the puncture of zona and plasma membrane and aspira...

Muhammad Yasin1, Romana Shahzadi1, Muhammad Riaz2, Mahideen Afridi2, Wajya Ajmal3, Obaid Ur Rehman2, Nazia Rehman3, Ghulam Muhammad Ali1,3, Muhammad Ramzan Khan1,2,3* 

..., respectively. The phylogenetic reconstruction of SHP1 and SHP2 homologs from other species conglomerated BnSHP1-like and BnSHP2-like into their respective clades. Semi-quantitative RT-PCR revealed overlapping expression of both the BnSHP1-like and BnSHP2-like transcripts in flower and siliques while no expression in the leaf tissues was observed. A strong expression for FUL gene was detectable in mature silique and silique from upper portion of plant as comp...

Tahira Jatt1, Ghulam Sarwar Markhand1, Lane Rayburn2, Ray Ming3, Mushtaque Ahmed Jatoi1 and Ameer Ahmed Mirbahar1,4* 

...cause of scarcity of cytogenetic knowledge. Khairpur district in Sindh is known as the biodiversity center of date palm. Investigations were carried on the chromosome number and karyotype one of the seven indigenous commercial date palm varieties and four wild type date palm of Sindh province, Pakistan by using the traditional Fuelgen squash method. Results indicated that all seven commercial varieties and four wild types of date palm are diploid in nature and...

Amany Adel, Wesam Mady*, Zienab Mosad, Fatema Amer, Asmaa Shaaban, Dalia Said, Marwa Ali Abdel Maged, Abdel-Satar Arafa, Mohamed Kamal Morsi, Mohamed Khalifa Hassan 

...etically, the genetic sequence for the Hemagglutinin (HA) and Neuraminidase (NA) of circulating Egyptian viruses are belonging to the Middle East G1- like viruses in group B that were closely related to each other and scattered phylogenetically in different subgroups with the presence of variant viruses in the quail during 2015. 

...
Zhimin Yuan1, Yan Yu2, Yanmei Wang1, Yanzhen Bu1,* and Hongxing Niu1,*
..., respectively. The phylogenetic analyses showed that the gastrointestinal bacteria were mainly classified into five phyla, dominated by Proteobacteria (27.8% in stomach and 39.7% in intestine) and Firmicutes (59.5% in stomach and 12.7% in intestine). Enterococcus and Bacillus were the two dominant bacterial genera in the stomach, accounting for 46.1% and 7.4% of total bacteria, respectively. Sphingomonas and Mycobacterium were the ...

Amany Adel, Wesam Mady*, Zienab Mosaad, Fatma Amer, Asmaa Shaaban, Dalia Said, Marwa Ali, Abdel-Satar Arafa, Mohamed Kamal Morsi and Mohamed Khalifa Hassan  

...etically, the genetic sequence for the Hemagglutinin (HA) of 38 viruses circulating Egyptian viruses during 2015 and 2016 were belonging to the Middle East G1- like viruses and scattered phylogenetically in different subgroups with the presence of variant viruses in the quail during 2015.  

...

Asad Ullah Khan1, Wiqar Ahmad1*, Amir Raza2, Farmanullah Khan3 and Muhammad Sharif3 

Ali Mahmoud Zanaty, Naglaa Mohammed Hagag, Neveen Rabie, Mahmoud Saied, Karim Selim, Saad A. Mousa, Azhar Gaber Shalaby, Abdel-Sattar Arafa and Mohamed Khalifa Hassan 

...ial fusion protein. Phylogenetic analysis revealed that 20 samples are genotyped as very virulent NDVclass II of genotype VIIb, 4 samples were of high identity (94%-100%) with NDV class II of genotype II (vaccine strain) and 1 sample was phylogenetically related to NDV class II of genotype I with 98% identity. Furthermore, the intracerebral pathogenicity index (ICPI) for selected 5 virulent viruses reveals velogenic features...
Jie Zhang1,2,*, Baradi Waryani3,4 and Qihai Zhou2,*
...t for Tibet Plantae. The genetic diversity and molecular invasive mechanism are urgently needed for both native and invasive populations. Present investigation details 12 polymorphic microsatellite loci and 12 to 26 alleles per locus denoted. Whereas, d expected and observed heterozygosity values ranged from 0.902 to 0.972 and 0.750 to 0.958, respectively. Moreover, the polymorphic information content (PIC) values ranged from 0.869 to 0.949. The cross-species ...
Jingchen Chen, Zhaochao Deng and Zhiqiang Han*
...lete mtDNA sequence, the genetic distance between the two lineages is 0.064. Results show that the total sequence lengths of the three samples of A. personatus mitochondrial genome are 16,537 bp (AP1), 16,536 bp (AP2) and 16,537 bp (AP3), where the base composition has biases of high A+T and low G+C contents, which are typical to the structural composition of vertebrates. Moreover, the two promoters (ATG, GTG) and four terminators (TAG, AGG, TAA, T or T...
Lusha Liu1,*, Lei Cheng2, Xingqi Zhang3, Yulong Li1 and Jianping Jiang1,*
...to design primers and do genetic analysis in the Sichuan basin population of M. fissipes. Of all, 35 primer pairs (57.38%) successfully amplificated in M. fissipes, of which 14 (40.00%) were polymorphism. The observed and expected heterozygosity in the test population ranged from 0.02 to 0.92 and from 0.02 to 0.62, respectively. High transferability rates were detected in M. butleri (37.14%), M. heymonsi (45.71%), M. pulchra ...

Yasir Ali1,2*, Bashir Ahmad1, Naqeebullah Jogezai3 and Adil Hussain

...sp. MG687270. Based upon genetic signatures and physiological parameters, our strain presented less than 79% of homology to Stenotrophomonas sp. and Pseudomonas sp. which makes it a novel species. Both of the strains were non-pathogenic and potentially industrial strains for the production of thermolabile protease and alkaliphilic lipase 

...

Wesam Mady*, Nahed Yehia, Mohammed Hassan Elhussieny, Azhar Gaber Shalaby, Moataz Mohamed El Sayed, Neveen Rabie Bakry, Asmaa Shaaban, Abdel Satar Arafa, Afaf Abdel Baset Mahmoud and Wafaa Mohammed Hassan 

...ve samples and they were genetically characterized as HPAIV H5N8 belongs to clade 2.3.4.4b. Except three isolates in 2018 belongs to clade 2.3.4.4A, the phylogenetic analysis revealed that our sequenced viruses were clustered together in group B Russian like reassortant H5N8 viruses of clade 2.3.4.4.  

...
Yan-Bin Zhu1, Wang-Dui Basang1, Zhan-Dui Pingcuo1, Yang-Ji Cidan1, Sang Luo1, Dun-Zhu Luosang1, Yang-La Dawa1 and Guang-Xin E2,3,* 
...ent study was to perform genetic diversity and population structure estimation on 382 individuals from seven Chinese Tibetan ecotype yak population using twenty-one microsatellites. The results revealed that the HO ranged from 0.4854±0.0194 in NNV to 0.6086±0.0267 in YRY, and the NA ranged from 3.86±1.98 in XMY to 6.05±3.37 in NNV. The least number of markers which deviated from Hardy-Weinberg equilibrium within the population was W...
Aqsa Javaid and Nageen Hussain*
...uencing was done through genetic analyzer (3500 ABI). No mutation was observed in FOXP3 gene exon 1of Pakistani HIV patients.
...
Ghaleb Adwan*, Sami Bdir and Kamel Adwan
...intermediate hosts for digenetic trematode cercariae. Digenean larvae were obtained from M. praemorsa snails that were collected from Palestine. Cercaria melanopsi palestinia I and III were identified as a xiphidiocercaria belonging to the microcotylae sub-group and as a microcercous cercaria, respectively. Phylogenetic analyses based on sequences of the ITS2 region of the nuclear rDNA revealed that C. melan...
Nida Zia1, *, Ayesha Maqbool2, Muhammad Safdar3, Umm-I-Habiba1, Altaf Mehmood4, Muhammad Usman5, Zahid Iqbal6, Javed Iqbal6, Shahid Mehmood6, Amanullah Khan7 and Sajid Umar8
...licons were sequenced to genetically identify the FAdVs. Phylogenetic analysis revealed two distinct clusters among FAdVs. One cluster containing 3 strains belonged to the FAdV- C species and serotyped as FAdV-4 and showed close proximity at the nucleotide level. The other cluster containing three strains belonged to the FAdV-D species and serotyped as FAdV-11. Furthermore, the sequencing analysis of detected field strains r...
Jam Nazeer Ahmad1*, Taiba Sharif3, Samina J.N. Ahmad2, Sumaira Maqsood4 and Fariha Zafar3 
...gene based primers. Phylogenetic analysis was also complemented to differentiate fruit flies identified from other countries of the world. The sequencing results and phylogenetic analysis of collected specimens indicated that sequences of B. dorsalis, B. cucurbitae and B. zonata have 99-100% similarity with fruit flies reported from other countries. This is the first report of molecular identification and chara...
Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1Muhammad Faheem Malik3 and Muhammad Idrees4

 

... RNA. The extensive phylogenetic analysis on the basis of partial helicase domain (ORF1) and capsid protein (ORF2) revealed clustering of Pakistani aHEV (Pak aHEV) strains with members of Orthohepevirus B species. However, Pak aHEV strains were highly divergent from other known members within Orthohepevirus B suggesting as novel aHEV strains circulating in the population of layer chickens in the country. Detection of HEV in layer chickens ...

Wajid Khan* and Raziuddin 

...conducted to investigate genetic variability and heterosis in eight rapeseed genotypes for maturity, morphological and yield traits. The eight parental genotypes i.e., AUP-01, AUP-05, AUP-07, AUP-08, AUP-10, AUP-13, AUP-18 and AUP-21 were crossed in full diallel fashion to generate 56 F1 crosses. These parental genotypes and their F1 hybrids were evaluated during 2015/16 in the field at the University of Agriculture, Peshawar, Pakistan. Mean squares revealed s...
Huaisheng Zhang1, Pengfei Li2, Huajun Wen2, Guangming Tian1, Hui Chen1, Lu Zhang1 and Jianqiang Zhu1,*
...amics, and biological or genetic characteristics of Père David’s deer in detail, review the research status and progress and examine the trends in the development and direction of Milu research in China, which should provide a reference for the healthy development and protection of the Milu population.
...
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
Yacheng Hu1,2, Xueqing Liu1,2, Jing Yang1,2, Kan Xiao1,2, Binzhong Wang1,2 and Hejun Du1,2,*
...useful tool to study the genetic diversity of Dabry’s sturgeon.
...
Irfan Irshad1, Asim Aslam1,*, Muhammad Yasin Tipu1, Kamran Ashraf2, Beenish Zahid3 and Abdul Wajid4
...have been pathotyped and genetically characterized from vaccinated birds collected between 2013-14. A phylogenetic analysis revealed that all the isolates belonged to sub-genotype VIIi with high similarity of 97.9 to 99.8% with similar viruses in this clade. Analysis of the hemagglutinin (HA) gene sequences of the two AIV was performed and phylogenetic analysis reveals
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Shahid Nabeel1 and Abu Saeed Hashmi2
...iv>The identification of genetic polymorphisms in genes enable us to estimate biological similarities and obtain a better perspective of quantitative traits that play key roles in development and regulating growth. The present study was conducted to characterize genetic variability in the growth hormone (GH) and growth hormone receptor (GHR) of Camelus dromedarius. GH is secreted and synthesized from somatotroph cells...
Shanshan Cai1, Tianxiang Gao2, Binlun Yan3, Aiyi Zhu1 and Xiumei Zhang1,2,*
...results also showed high genetic diversity and a certain degree of heterozygote deficiency of natural swimming crab populations. The advancing technology and its unique advantages will make molecular markers as novel and highly-efficient approaches for assessment of stock enhancement.
...
Ihsanullah*, Muhammad Subhan Qureshi, Sohail Akhtar and Syed Muhammad Suhail
...g. The crossbred cows of genetic group HF 62.5% tended to show shortest ovulation intervals and the summer calvers showed significantly highest intervals. Based on results of this study it is suggested that cows calving in summer show lowest calving interval. Crossbred cows with 50% exotic blood showed earliest age at first calving, although non-significant. Crossbred dairy cows showed a constant upward trend in peak daily milk yield from first through sixth p...

Adewumi Moses Olubusuyi1, Ogunsakin Temitope Rachael1, Ogunrombi Sefiu Babatunde1, Ojeamiren Iduda1, Olawole Alaba Samuel1, Faleye Temitope Oluwasegun Cephas1,2* and Adeniji Johnson Adekunle1,3 

...icon sequencing and phylogenetic analysis. Eleven and one of the 30 pools of RD and L20B cell culture supernatants, respectively, were positive for the RT-snPCR. Nine of the 11 amplicons from RD and the only from L20B were sequenced and identified as seven EV types; Coxsackievirus A4 (CV-A4), CV-A6, CV-A13, CV-A17 (both RD and L20B), CV-B2, Echovirus 9 (E9) and Enterovirus A76 (EV-A76). Our findings suggest some enteroviruses are present in and might be replic...
Tasleem Akhtar1,2,Ghazanfar Ali1,*, Nuzhat Shafi2 and Abdul Rauf2
...;s F* indicated that the genetic variations in 16S rRNA between species were not neutral which reflect the excess of external mutations. Results indicate that 16S rRNA gene is unable to fully resolve the inter-relationship of Schizothoracinae species.
...
Azra1* and Shaukat Hussain2
... find out new sources of genetic resistance in maize genotypes against southern corn leaf blight. The experiment was laid out as randomized complete block design with three replications. Data on disease severity and grain yield were recorded. There were not significant differences in disease severity among maize genotypes for individual years. However, significant differences were recorded amongst the genotypes when the data were pooled over two years. Based o...
Beenish Zahid*1,2,7, Javed Iqbal Qazi2, Amir Zohaib2, Asim Aslam3, Raheela Akhter3, Haleema Sadia4, Qurat ul Ain5, Razia Sultana6, Irfan Irshad3 and Sobia Alyas7
...argeted the F gene. Phylogenetic tree was constructed. It was concluded that virulent strains were existed in ducks and more than one genotype of NDV was prevalent in ducks.
...
Jiaxin Guo1, Xiumei Zhang1,2, Xiaoyan Wang2, Liqin Liu2 and Tianxiang Gao1,*
...evaluation germplasm and genetic composition in the further research.
...

Muhammad Khuram Razzaq1,2*, Saeed Rauf1, Mohsin Khurshid3, Shahid Iqbal1,4, Javaid Akhter Bhat2, Ayaz Farzand5, Adeel Riaz1,6, Guangnan Xing2 and Junyi Gai

 

...r, magnitude and type of genetic variation associated with pollen viability also important in improving the trait and breeding procedures to be adapted for the improvement of trait per se. Genetic studies results under heat stress in sunflower showed additive to partial dominance towards higher pollen viability in the inheritance showing that trait can be improved through selection in segregating generations. In this review,...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1 
...y serve as authoritative genetic marker for selective improvement of growth traits in camel. These results suggest that Marecha camel has genetic variability in the IGF-1 and may be helpful for preservation strategies and future selection programs.  

 

...
Memis Ozdemir
...locus and is a potential genetic marker that can be used in the improvement of production traits in dairy cattle, because prolactin has an important regulatory role in the development of the mammary glands, the secretion of milk and the expression of milk protein genes. It has been seen many studies about Prl/RsaI polymorphism found in the exon 3 or exon 4 region of bovine prolactin gene in the literature. The aim of this study was to determine whether ...

Muhammad Abdul Rahman1*, Abdul Saboor1, Gulnaz Hameed1, Ghulam Bilal2 and Farooq Tanwir3 

.... Experts ranked lack of genetic research and fodder security on top among various critical factors that drive and increase occurrence of security hazards to health and production of dairy animals. Lack of labs to identify diseases, sensitiveness of buffaloes to temperature, slow and reduced weight gain and effect on birth and death rate are weighted high as threat to farm animal health while India-Pakistan ties on water, water scarcity, high water requirement...

Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2 

Muhammad Abdul Qayyum1*, Farhat Bashir2, Muhammad Mudassar Maqbool3, Anser Ali3, Saqib Bashir1 and Qaiser Abbas4 

...s of linseed expressed a genetic vigour to combat salinity stress by restricting Na+ entry at root level and by using this trait through biotechnology, plant breeders can produce salt tolerant crops. 

...

Khaled Kaboudi* 

...s about epidemiology and genetic characteristics are still little known. This review summarized the epidemiological situation of H9N2 subtype in poultry in Algeria, Egypt, Libya, Morocco, Sudan and Tunisia. Human infection was reported in Egypt and serosurveys programs showed high positivity, particularly in poultry workers. Genetic analysis demonstrated that the most of H9N2 viruses circulating in North Africa were among th...
Fizza Anwar1, Muddassar Zafar1*, Zahid Anwar1, Raja Tahir Mehmood2
...rowth. In addition, phylogenetic tree was also constructed using 18s rRNA gene of A. niger and gene clusters of secondary metabolites were determined through bioinformatics tools. 
...
Hafiza Sadaf Zahra1, Asia Iqbal2, Sayyeda Hira Hassan1, Hafiz Abdullah Shakir1*, Muhammad Khan1*, Muhammad Irfan3, Chaman Ara1, Shaukat Ali4
...ributed due to inherited genetic mutations while remaining 90% cancer cases are associated with environmental factors. Artificial light at night (ALAN) is considered one of the major environmental risk factors for breast cancer. It inhibits production of melatonin (MLT) from pineal gland which results in abnormal epigenetic changes that relates with an increased risk of BC. The most important ALAN-mediated epi
Shahid Sherzada1,2,4,*, Muhammad Naeem Khan4, Masroor Ellahi Babar2, Muhammad Idrees3, Abdul Wajid2, Muhammad Nauman Sharif3, Muhammad Shahzad Iqbal3, Iram Amin3 and Muhammad Shahid3
...ongenric and confamilial genetic distances were 0.22%, 6.71% and 12.33%, respectively. Findings of the present study revealed that DNA barcoding using COI gene is an efficient molecular technique for the exact identification of typical fish species.
...
Syed Maaz Nadeem1*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh1, Arfan Ahmad1* and Muhammad Anees2
...ce of Pakistan. The phylogenetic analysis clustered study strains of CAV in group II similar to those reported previously from China. These findings are helpful for an understanding of currently circulating strains of CAV; however, they require further analysis to intervene in necessary strategies to avoid future outbreaks in commercial poultry.
...
Peng Chen1,2, Zuhao Huang3,Chaoying Zhu1, Yuqing Han1, Zhifeng Xu1
Guanglong Sun1, Zhen Zhang1, Dongqin Zhao4, Gang Ge1 and Luzhang Ruan1*
... lowest was Cys. In phylogenetic analyses, Gruiformes included Grui and Ralli, and Charadriformes included Charadrii, Lari and Scolopaci. The genus Porzana was closest to Porphyrio. Gallirallus striatus and Lewinia muelleri consisted a sister group, while Rallus aquaticus was a separate branch. Hydrophasianus chirurgus (Charadriiformes: Jacanidae) was closely related to Rostratulidae. According to the estimation of div...

Sana Munir1, Muhammad Kamran Qureshi1*, Ahmad Naeem Shahzad2, Ismat Nawaz3, Muhammad Shahzad Anjam4, Sumaira Rasul4 and Muhammad Asif Zulfiqar5 

...y;">To estimate level of genetic diversity various cotton genotypes, inter and intraspecific hybrids were assessed for yield, yield contributing and fiber traits using various multivariate analysis such as principle component analysis and linkage cluster analysis. Principle component analysis (PCA) exhibited that first four PC out of nineteen PC, with eigen value >1, contributed about 80.326 % of total variability. Positive contribution towards PC1 was give...
Javeria Zafar1, Asif Nadeem1*, Maryam Javed1, Fehmeeda Fatima1, Wasim Shehzad1, Ghulam Abbas1, Rajput Zahid Iqbal2 and Muhammad Muddassir Ali1
...and to determin its phylogenetic relationship with other capra species. Blood samples were collected from Kirthar National Park, Sindh, Pakistan. PCR product was sequenced bi-directionally using dideoxy chain termination method after mitochondrial ATPase8/6 genes amplification. Polymorphism, Genetic diversity and Phylogenetic analysis were carried out by the MUSCLE, DnaSP and MEGA6 ...

Iffat Nawaz1*, Farhatullah1, Fida Muhammad1, Sajid Ali2 and Ghulam Muhammad Ali

...e an important source of genetic diversity. A total of 96 indigenous accessions of common beans (Phaseolus vulgaris L.) were collected from three different areas of Himalaya region of Pakistan. In all accessions, 78.1% were collected from Khyber Pakhtunkhwa while 11.5% from Kashmir and 10.4% from Gilgit Baltistan. Primary evaluation of seed characteristics was done according to International Board of Plant Genetic Resources ...
Umm-i-Habiba1,*, Ayesha Maqbool2, Muhammad Safdar3, Nida Zia1Altaf Mehmood4, Muhammad Usman5, Mehwish Sharif6, Amanullah Khan7 and Sajid Umar8
...rom these outbreaks were genetically characterized based on hyper-variable region of the VP2 gene. IBDV strains were grouped into two distinct clusters on the basis of nucleotide sequences of hyper-variable region of the VP2 gene. According to phylogenetic analysis, 5 IBDV strains showed characteristic amino acid signatures in the VP2 gene (A222, I242, I256, I294, S299) and classified as vv...
Sajida Rasool1, Saba Irshad1*, Neelam Saba1, Mehak Fiaz1Muhammad Sajid Hussain2, MuhammadWajid Hussain3 and Peter Nürnberg2

 

...gia (HSP) are a group of genetically and clinically diverse neurodegenerative ailments with distinguishing feature of lower limbs spasticity and upper motor neuron degeneration. BICD2 is an adaptor protein which regulates the cellular trafficking of cargo molecules crucial for motor neuron growth and maturation. In present study, a Pakistani family of HSP penetrating in autosomal recessive pattern was ascertained. Patients presented spasticity and stiffness of...
Mustafa Koyun1*and Şenol Çelik2
... D. spathaceum (digenetic trematode) on the weight and sex of the host fish (Mastacembelus mastacembelus) by using some data mining algorithms. During this study, the fish weight (g) and length (mm) of 122 fish were measured. In addition, the distribution of M. heteranchorus and U. pictorum in each lamella of the gills and the distribution of D. spathaceum in the right and left eye lenses were evaluated. Two different algorit...

Basharat Hussain Shah*, Farrukh Siyar Hamid, Shams ul Islam, Naveed Ahmed, Fayaz Ahmad, Noorullah Khan and Qamar uz Zaman. 

...to explore the amount of genetic variability that may exist in the available genetic material and to study the variability of yield and yield attributes in advance rice lines. For this purpose, fourteen elite rice lines namely Line-21, Line-25, Line-28, Line-31, Line-37, Line-46, Line-48, Line-50, Line-64, Line-73, Line-80, Line-128, Line-130 and Line-140 developed in the background of Basmati-385 by Department of

Naglaa M. Hagag1*, Mervat E. Hamdy1, Mary A. Sargious1, Sara M. Elnomrosy1, Nahed A. Ahmed1, Ayman A. Hamed1, Ahmed R. Habashi1, Essam I. Ibrahiem1, Mahmoud A. Abdel-Hakim2 and Momtaz A. Shahein

... in Egypt. Molecular and genetic characterization indicates that serotype SAT2 is widespread (in 96 cases), while only four cases of Serotype O were detected. Phylogenetic analysis of the study sequences indicates that the circulating FMDV serotype SAT2 viruses were homogeneous and related to Topotype VII. Importantly, the newly emerged viruses were closely related to strains isolated from Libya in 2012 (Topotype VII, lineag...
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
...lor traits and the color genetic mechanism of Nubian goats.
...

Ajmalud Din1*, Munir Ahmad2, Fahad Masoud Watto2, Sheraz Ahmed1, Imtiaz Ali1 and Muhammad Kausar Nawaz Shah

...eat genotypes of diverse genetic makeup were assessed in transparent polythene bags (6 × 27 inches), and filled them with sterile, homogenized mixture of loam, sand and compost soil. Two factorial Completely Randomized Design was followed with three replications and two treatments. The experiment was conducted during 2015-16 in the experimental site of the Department of Plant Breeding and Genetics, PMAS Arid Agricultur...
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
...88 gene is linked to genetic resistance to Salmonella Pullorum infection and may provide an important reference for the marker-assisted selection of chickens during disease-resistance breeding.
...
Muhammad Asif Gondal, Qazal Waheed, Sana Tariq, Waseem Haider, Aisha Khan, Qudsia Rasib and Haroon Ahmed*
...n-terms of ecology, phylogenetic analysis, spatio-temporal variation and diversity.
...
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...in only 6 isolates. Phylogenetic analysis of the tet (O) gene sequences recovered from the C. jejuni isolates revealed that the strains isolated from chickens and drinking water from the same farm were identical. However, the sequence ofthe tet (O) gene from human isolates was highly similar to that from drinking water isolates. Our findings highlight the presence of MDR Campylobacter strains in chickens and the role of drinking wat...

Mohammad Aquil Siddiqui1*, Muhammad Tahir Khan1, Ghulam Shah Nizamani1, Shafquat Yasmeen1, Imtiaz Ahmed Khan1, Abdullah Khatri1 and Nighat Seema Soomro2 

...role in manipulating the genetic structure of the plants as an indispensable tool for crop improvement. This field study was initiated to evaluate three potential mutant lines of lentil against their parent (M-85) and two check varieties (NIA-Masoor-05 and NIA-Masoor-16). The pooled data of the crop, after two years of evaluation, indicated the earliest maturity in the mutant AEL-40/30 (92.0 days). Plant height was observed to be low in all the studied mutant ...

Muhammad Ilyas1, Sardar Ali Khan1, Shahid Iqbal Awan1, Shafiq-ur-Rehman1, Waqar Ahmed1, Muhammad Riaz Khan2, Raja Mohib Muazzam Naz2, Muhammad Mohib Ullah Khan3 and Sumaira Hafeez1* 

...t traits. Broad range of genetic diversity was shown by the inbred lines and displayed different levels of drought tolerance. Four inbred lines were selected i.e., VDR-51, DR3-126, DR-37 and 5CDR-53. Crosses were attempted between the selected inbred lines. Six generations as P1, P2, F1, F2, BC1 and BC2 were prepared for both the crosses for the estimation of gene action under generation mean analysis and planted in a triplicated Randomized Complete Block Desi...

Muhammad Ilyas1*, Sardar Ali Khan1, Shahid Iqbal Awan1, Shafiq-ur-Rehman1, Muhammad Riaz Khan2 and Sumaira Hafeez1 

...plained a broad range of genetic variability and displayed different levels of drought tolerance. Four inbred lines were selected i.e. VDR-51, DR3-126, DR-37 and 5CDR-53. Crosses were done among the selected inbred lines. Six generations as P1, P2, F1, F2, BC1 and BC2 were prepared for both the crosses to calculate heterosis and inbreeding depression for Randomized Complete Block Design under split plot arrangement. Highly significant inbreeding depression was...
Muhammad Younis Laghari1,2, Zixia Zhao1, Yiwen Gong3, Punhal Lashari1,2, Shangqi Li1, Jian Xu1 , Ateeque Rahman Khooharo4, Jiongtang Li1,* and Yan Zhang1*
...d from the mainland, the genetic diversity in Hainan island is different to that in the mainland. One of fifty sequences isolated from SINE element was polymorphic, including six haplotypes with an average sequence divergence of 11.03% (SE = 0.78%). Four haplotypes (MF177501-MF177504) were shared in these four population. However, two specific haplotypes (MF177499-MF177500) were only observed in Hainan population. To simplify procedure and improve effectivenes...
Savas Atasever*, Huseyin Erdem and Ibrahim Cihangir Okuyucu
...were not affected by non-genetic factors (farm, parity and stage of lactation), moderate correlation coefficient (r=0.536) was calculated between two traits. Routinely recording productive traits and rechecking feeding management for elevating RFS in the farms may be suggested in the investigated farms.
...
Jun Yan Bai1*, Xiao Ping Jia2, Xiao Hong Wu1, Guang Lu Li1, Heng Cao1, Xue Yan Fu1 and Kun Peng Shi1
...te quail has the closest genetic relationship with Japan quail, partridge, and mallard, which are clustered in one group.
...
Amna Shoaib*, Zoia Arshad Awan and Naureen Akhtar
...i> A. flavus are two genetically distinct strains. Furthermore, both strains were qualitatively analyzed for aflatoxins (AFB1 and AFB2) production by thin-layer chromatography (TLC). A polyphasic strategy as adopted for the current study is a reliable and reproducible means to differentiate A. minisclerotigenes from A. flavus, indeed essential in interpretations of taxonomic and nomenclature of A. flavus group that may allow prior diag...
Mubashra Salim1, Omeira Ibrahim1, Hugo Vilhena2,3,4, Carla Maia5, André Pereira5, Maria Shahzeen1, Shabana Kalsoom1, Asim K. Mahmood6 and Furhan Iqbal1*
...nts. Sequencing and phylogenetic analysis was performed in M. haemofelis infected samples. Analysis of risk factors revealed that risk of M. haemofelis and of C. Mycoplasma haemominutum infection was significantly higher during summer months than during the winter season (P ≤ 0.01 in both agents). Cats older than one year of age were significantly more predisposed to C. Mycoplasma haemominutum infection than younger cats (P ≤ ...
Mehtap Bayir*
...fish and tetrapods. Phylogenetic clustering was seen between sod genes in fugu and their orthologs. Finally, highly conserved gene synteny was determined between fugu sod genes and their orthologs from teleost fish and human.
...
Jiantong Feng, Xueping Wen, Yahong Guo, Yingying Ye*, Jiji Li and Baoying Guo
...rder to characterize the genetic diversity and population structure of M. lamarckii, a 750 bp region of mitochondrial COIII gene and an 822 bp region of 12S rRNA gene were sequenced and analyzed for 118 and 105 individuals from five populations (ZS, WZ, ZP, ST and ZJ) in the Southeast coastal areas of China, respectively. Results revealed that 33 haplotypes were defined in COIII gene, and 30 haplotypes were defined in 12S rRNA gene. The pairwise F
Gulnaz Afzal1, Ghulam Mustafa2*, Shakila Mushtaq3 and Amer Jamil4
...cation tool and for phylogenetic relationships of spiders. Despite being sympatric species, the origin and diversification of Araneid fauna in wheat fields are remarkably distinguished which may help to understand the biology, biogeography and the long-standing controversies of their systematic studies.
...
Yunfang Tian, Zeqin Fu, Jiantong Feng, Yahong Guo, Yingying Ye*, Jiji Li and Baoying Guo
...nfluenced the population genetic structure of marine mollusk organisms via promoting or limiting planktonic larval dispersal. The clam, Meretrix meretrix, is an important aquaculture species in the coastal regions of China. However, the previous studies of genetic information about M. meretrix were vague. None integrated research to reveal the population genetic and structure...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
...II) oocytes were parthenogenetically activated to evaluate pronuclear (PN) formation of parthenogenetic embryo and embryo development. The cytoplasmic maturation was examined by measuring the intracellular reactive oxygen species (ROS) and glutathione (GSH). DNA DSBs were examined by immunostaining of pi-H2AX, the marker of DNA DSBs. The results showed that the GVBD rates were similar in oocytes of young and aged mice. Polar...
Muhammad Rizwan Ashraf1, Asif Nadeem1,2, Eric Nelson Smith3, Maryam Javed1, Utpal Smart3, Tahir Yaqub4, Abu Saeed Hashmi1 and Panupong Thammachoti3
...t easy to elucidate phylogenetic relationships and evolutionary histories of different groups of organisms. This study is the first attempt to find the phylogenetic relationship and diversity through mitochondrial genes in saw-scaled viper from Pakistan. Tail tip biopsies of Saw-scaled vipers were used for amplification of mitochondrial genes fragments (ND4, 16S rRNA and 12S rRNA) through Polymerase Chain Reaction (PCR). Nuc...
Peimin Yang*, Zongyun Hu, Yixin Liu, Guanghai Jin and Lei Wang
...hina. To investigate the genetic variability and population structure of this species in its native range, we analysed variation in the mitochondrial cytochrome b (Cyt b) for 94 specimens collected from five locations. Sequence analysis showed that there were 50 polymorphic sites and 20 haplotypes among five populations, and the samples exhibited high haplotype diversity (h=0.8467) and low nucleotide diversity (Pi=0.0063).

Fazli Ahad1, Raziuddin1, Nazir Ahmad1,2*, Muhammad Nauman1, Touheed Iqbal3, Nabeel Khan1, Fazli Hameed4 and Quaid Hussain2 

...o estimate heritability, genetic advance, and correlation coefficient in a set of 31 rapeseed genotypes, comprised of 10 parental lines and their 21 F3 populations. These genotypes were evaluated in a randomized complete block design at The University of Agriculture-Peshawar-Khyber Pakhtunkhwa-Pakistan with three repeats. Significant differences were recorded for days to 50% flowering, plant height, primary branches plant-1, main raceme length, pods main racem...
Maria Khalid1, Tanveer Hussain1*, Zahid Farooq2, Kamran Abbas1 and Masroor Ellahi Babar1  
...ed us to investigate its genetic diversity and phylogeny using mitochondrial DNA, Cyt-b and Cox1 genes. A total of 749bp of Cox1 and 472 bp of Cyt-b complete coding regions of both genes were amplified by PCR followed by sequencing. The sequences were aligned and edited using Bio-Edit software and single nucleotide polymorphisms (SNPs) were identified. The boot strapped Neighbor joining tree constructed from MEGA7 explained the
Masroor Ellahi Babar*
...lymorphic and it is phylogeneticaly linked with other mammals. The analyses of the sequence of 5︠ flanking region in various breeds reveals the presence of different polymorphic regions includes cysteine nucleotide deletion, AT bases insertion and transvertions (C>G; G>T). Deletion of A bases at c.1-1075 and hetrozygosity (CT) at the c1-1046 were analyzed along with some other SNPs are very important. These evidences points towards the change of expres...
Qingzhan Ma1, Bo Wu1, Jianping Jiang2 and Zhaobin Song1,3*
...n the present study, the genetic variability and relationships of the domestic populations of channel catfish (Ictalurus punctatus) in Sichuan Province, China were studied to determine their genetic background and provide essential data for its artificial breeding. Eight microsatellite markers were used to analyze genetic characteristics of eight channel catfish populations (POPA, P...

Muhammad Safdar Hussain1*, Muhammad Farrakh Nawaz2, Muhammad Ayyoub Tanvir2 and Noor-E-Hira

...Eucalyptus camaldulensis genetically and physiologically to bring modification in this plant that can make it less water demanding to conserve water. 

...
Lei Wang1, Lu Yang2, Lei Luo1, Yaqi Gao1*, Jian Gao1 and Ping Liu1
...heoretical basis for the genetic manipulation of A. cinerarius. Illumina RNA-Seq was used to perform massive sequencing of 18 cDNA samples derived from male adults, female adults, and pupa of A. cinerarius in southern and northern Xinjiang. We extracted total RNA from 18 samples and synthesized cDNA via reverse transcription. Then, Illumina sequencing was performed and unigenes were identified after assembly and clustering. The resulting unigenes...
Mahvish Rajput1, Abdullah G. Arijo1, Muhammad Bachal Bhutto1Rehana Shahnawaz Buriro2, Javaid Ali Gadahi1, Muhammad Naeem3 and Zubair Ahmed Laghari1*
...ed for constructing phylogenetic tree. The sequence and evolutionary relationship analysis indicate that all isolates were belong to the same species and were clustered together with Paramphistomum epiclitum. Taken together, this study provides the baseline data for the prevalence of rumen fluke infection in southeastern Pakistan and their molecular characterization using rDNA ITS-2+ region which serve as useful genetic
Sara Zafar* and Asmatullah
...cially during the organo-genetic period, can cause harm to the developing embryo and Pomegranate juice significantly reduced the detrimental effects caused by atenolol.
...

Muhammad Iqbal1, Sami Ul-Allah2*, Muhammad Naeem1, Muhammad Ijaz2 and Muhammad Qadir Ahmad3 

...fects of water stress on genetic variability of root and shoot traits and association of the seed traits with seedling vigor. 15 cotton cultivars were studied at two levels of irrigation during 2014-15. Results showed highly significant (p≤0.05) genotypic differences for seed and root‒shoot traits. Reduction in fresh weight of different cultivars due to water stress ranged 14.74 to 29.81% and reduction in dry weight due to water stress range 12.52 to 43.3...
Muhammad Mohibullah1*, Mehran1, Sundas Batool1, Muhammad Amin1, Zakiullah1, Muhammad Ilyas2, Irfanullah1, Abdur Rehman1 and Sardar Ali3
...d sense heritability and genetic gain values were also noted in seed yield plant-1 and 2ndry branches plant-1 ranging from 71 to 91% and 36.00 to 60.00%, respectively. Therefore, the above promising accessions have greater genetic variability and sufficient information in further breeding programs to introduce ideotype chickpea genotypes.
...

Hasaan Aziz*, Fariha Altaaf, Attia Bashir, Abdul Basit, Syed Anam Masood Gardazi and Farah Haider 

...e, we sequenced and phylogenetically analysed the complete hemagglutinin gene of CDV from a male dog (14 months old) exhibiting clinically suggestive symptoms. With a characteristic amino acid residue for dog at Y549, sequence analysis showed geographically distinct pattern clustering the study strain to clade Asia-2 but clearly distinct from vaccine strains. Though limited and preliminary data, the findings enhance our knowledge towards CDVs circulating in Pa...

Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5* 

...disparities and the phylogenetic analysis of unusual human rotavirus VP4 genotype detected in Moroccan children fully vaccinated with Rotarix™. After RNA virus extraction, the rotavirus VP7 and VP4 genes were amplified. The DNA was purified, sequenced and genotypes were determined using the RotaC online classification tool. A phylogenetic tree was constructed using the Maximum Likelihood method applying the Tamura 3-pa...

Olotu Titilayo Mabel*, Adeosun Idowu Jesulayomi, Kaka Mary Oluwatosin and Oladipo Elijah Kolawole 

...ed using MEGA 6 for phylogenetic tree. The sensitivity to antiviral drugs was analyzed using the Stanford University HIV Drug Resistance Database. Our results revealed that Kenyan strain (SN-042062), Ugandan strain (DR138-13), Sudanese strain (G-01035) and Nigerian strain (NGA-INF-2016-618) have similar branch length. The susceptibility percentage for each antiviral drug ranged from 82.35% (Tenofovir) to 64.70% (Rilpivirine) while and resistance percentage ran...

Joseph Anejo-Okopi1*, Ocheme Julius Okojokwu1 and Onyemocho Audu2 

...anding the extent of HCV genetic diversity is important for treatment and development of vaccine. Among the routes of HCV transmission; intravenous drug addiction or injection drug use accounts for the majority of many cases apart from hemodialysis patients, unprotected sexual intercourse, blood transfusion and vertical transmission. Therefore, there is a need for government commitment for HCV elimination program through making the DAAs available and its affor...

Hafiz Muhammad Imran Javed* and Mushtaq Ahmed 

...tudy compared the select genetic markers of the vaccine and field strains originating from the field outbreaks for phylogenetic analysis, nucleotide and deduced amino acid sequence analyses, and a comparison of three-dimensional protein structures. The N gene ORF was 1578 nucleotide long with a single open reading frame encoding 526 amino acids. Similarly, the ORF of F gene was 1641 nucleotide long which encoded 547 amino ac...

Paul Oludare Adetunji* and Safiriyu Idowu Ola 

... for rearing and further genetic improvement. Eighteen cockerels of three plumage colours (brown, black and barred) were divided into 2 replicates with 3 birds per replicate. The study lasted for 38 weeks (Feb.-Nov., 2019). Data were taken on the onset of puberty parameters (age at first sperm cell and puberty) from 16-22 weeks of age, semen quality parameters (semen volume, semen concentration, sperm output, sperm motility and sperm abnormalities) from 26-52 ...

 Waqas Javaid1*, Adnan Tariq1, Iftikhar Hussain2, Sahar Noor2

AN EVOLUTIONARY APPROACH FOR CMS DESIGN

Muhammad Naeem1*, Sahib Khan2, Nasir Ahmad3

A DISTRIBUTED GENETIC ALGORITM AND A-PRIORI ALGORITHM FOR THE HUB AND FACILITY LOCATION PROBLEMS
... combine a-priori with a genetic algorithm (GA)
to solve two classical NP-hard location problems namely the Un-capacitated Single Allocation Problem (USAHLP)
and Un-capacitated Facility Location Problem (UFLP). A distributed model of the proposed algorithm has been
implemented. The performance of the algorithm has been evaluated with standard benchmark problems for USAHLP
and UFLP. Results have been found encouraging.

...

Muhammad Riaz1*, Naureen Akhtar1, Salik Nawaz Khan1, Muhammad Shakeel1,2 and Ateeq Tahir1

Neocosmospora rubicola: An Unrecorded Pathogen from Pakistan Causing Potato Stem Rot
... beta tubulin gene. Phylogenetic analysis was also conducted to determine the phylogenetic relationship of this species with other reported species of this genus. Pathogenic potential of the isolate was verified by artificially inoculating the spores of pathogen in healthy plants. Appearance of same symptoms and re-isolation of N. rubicola from infected tissue confirmed Koch’s pathogenicity postulates. Association of N...

 Muhammad Shahzad1, Arif Iqbal Umar1, Syed Hamad Shirazi1, Muhammad Tariq Pervez2*, Zakir Khan1, Waqas Yousaf1

HCDP: HEPATITIS C DATA BANK OF PAKISTAN
...l structure of phylogenetic tree. With the help of Format converter/Fasta generator tool user can convert sequence with formats of PhyLip, NEXUS, MSF, CLUSTAL and PIR into standard FASTA. It is also observed that genotype 3a (76%) is more prevalent followed by genotype 3 (13%). Geographic distribution reveals that rate of occurrence of HCV in Sindh and KPK is high with respect to other provinces. An annotated database of HCV genome sequences all...

Adnan Tariq,1 Iftikhar Hussain,2 Abdul Ghafoor,3 Sahar Noor2

AN EVOLUTIONARY ALGORITHM FOR JOB-SHOP SCHEDULING

 Faizullah Mahar1, Syed Saad Azhar Ali1, Abid Karim1

DESIGN OF FIXED ORDER ROBUST CONTROLLER USING H∞ -NORM AND EVOLUTIONARY TECHNIQUES: COMPARISONS AND PERFORMANCE ANALYSIS
...tionary techniques (i.e. genetic algorithm and immune algorithm) for the designing of a low, fixed order robust controller. To check the effectiveness of the proposed approach, resulting controller parameters are evaluated for performance analysis via extensive simulations. Simulation results and performance comparisons demonstrate the efficiency of proposed approaches.
...
Guang-Hui Tan, Yi-Yu Zhang*, Yuan-Yu Qin, Lei Wu and Jie-Zhang Li
...nged to medium levels of genetic diversity. Association analysis revealed that two SNPs were significant association with at least four of seven lipid indexes. Allele A of CDS 216 A>G locus and allele T of CDS 681 T>A locus was favorable to improving meat quality, respectively. Three haplotypes and six diplotypes were identified by the combination of two SNPs. Diplotypes had dominantly affected on tested lipid indexes except for IMF. Diplotype H1H1 was a...
Jam Nazeer Ahmad1,*, Samina J.N. Ahmad1,2,*, Mubashir Ahmad Malik1, Abid Ali1, Muhammad Ali3, Ejaz Ahmad1, Muhammad Tahir2 and Muhammad Ashraf2
...l, 1775). Molecular phylogenetic analysis and evolutionary divergence study also revealed the formation of same cluster in phylogenetic tree with that of specific subspecies S. g. gregaria which furtherdeviate genetically not only from closely related subspecies (S. g. flaviventris) but also other species reported from various countries. Migratory pattern of S. g. gregaria...
Yan Zhou1,2, Hai Xia Han1,2, Qiu Xia Lei1,2, Jin Bo Gao1,2, Wei Liu1,2, Fu Wei Li1,2, Jie Liu1,2 and Ding Guo Cao1,2*
...iplotypes based on three genetic mutations (NC_006127.2:g.8467G>A, NC_006127.2:g.12321G>A and NC_006127.2:g.13876A>G) were constructed, and the associations of diplotypes with reproduction traits were assessed, their effects on gene expression were evaluated also. As a result, three haplotypes H1 (G-G-A), H2 (G-G-G) and H3 (A-A-G) were obtained, H1 was the main haplotype with a frequency of 91.75%. The correlation analysis showed that diplotypes (H1H1...

Muhammad Jawad1*, Shahid Riaz Malik1, Rana Muhammad Atif2, Haris Ahmed2 and Muhammad Shahzad Afzal3

Species Identification of Gram Wilt Complex through ITS Region by PCR-RFLP Analysis
...cted to identify the polygenetic association of the pathogens of chickpea wilt complex. Universal Rice Primers based diversity analysis indicates high polymorphism within the collected wilt isolates. Primers ITS 1 and ITS 4 generated internal transcribed spacer fragments that were restricted four restriction enzymes (HhaeI, MboI, BsuRI, HinfI). PCR ITS-RFLP banding pattern identified two Fusarium species i.e. Fusarium oxysporum and Fusarium solani. In conclusi...
Sehrish Firyal1,*, Ali Raza Awan1, Muhammad Umair Latif1, Muhammad Tayyab1, Muhammad Wasim1, Shagufta Saeed1 and Imaad Rashid2

 

...l for taxonomic and phylogenetic analysis. These are bi-allelic molecular markers, easy to interpret and uniformly distributed within genomes. In this study cytochrome b (Cytb) gene based SNPs analysis was employed to genetically characterize the Pakistani domestic breed Lathy pigeon. Cytb haplotype for local domestic breed was developed. Homology analysis of Cytb gene revealed Columbia livia as c...

Rao Wali Muhammad1, Hafiz Muhammad Wasif Ali2*, Amir Hamza1, Muhammad Qadir Ahmad2, Abdul Qayyum2, Waqas Malik2 and Etrat Noor2

Estimation of Different Genetic Parameters in Various Safflower (Carthamus tinctorius L.) Genotypes under Field Condition
...d out to investigate the genetic estimates regarding various economic traits i.e. days to flowering, plant height, days to maturity, no. of primary branches per plant, no. of secondary branches per plant, pods per plant, 1000-seed weight and yield per plant of 200 safflower accessions. Analysis of variance and principal component analysis were carried out to estimate the extent of variability for studied parameters and to partition the germplasm into various c...

Moazam Ali1, Wajid Ali2, Ayhan Ceyhan2 and Zeeshan Ahmad Bhutta3*

Pigmentation Genome Influence in Animals and Human Interventions in its Course of Action
...dicted with mutations on genetic asset and production factors. The human desire of keeping variated colored animals advanced the genetic field as in many species (sheep, cattle, horses, camels, dogs, cats, pigs) new variants of coat pigmentation are achieved by generating mutation in MC1R and ASIP allele. A row of scientists is working on genome sequences and mutations for getting a better and healthier pigmentation besides ...
Muhammad Zahid Mengal1, Hamida Ali2, Raheela Asmat3, Muhammad Naeem1,4, Ferhat Abbas1, Abdul Samad1, Mohammad Zahid Mustafa1, Jannat Raza2 and Tauseef M. Asmat1*
...ost frequent rpoB genetic mutations were observed in codons 531/533 (Probe E, 78.3%) whereas the least within the sequence 511 (Probe A, 2.2%).
...

Khan Sher1*, Muhammad Subhan1, Muhammad Nisar2, Ali Hazrat2, Zahid Fazal1, Gul Rahim2, Imran Ahmad1, Riaz ul Haq1 and Shamia Bibi1

Genetic Diversity in Common Beans (Phaseolus vulgarus L.) Collected from Different Ecological Zones of Malakand Division (A Part of the Sino Japanese Region of Pakistan)
... to analyze the level of genetic diversity (GD) of bean landraces collected from diverse ecological zones of Malakand division, Khyber Pakhtunkhwa, Pakistan. The qualitative traits showed significant level of diversity like seed shape was determined by two alleles, seed color was defined by 13 numbers of alleles and four alleles were responsible for flower color. Similarly, the quantitative traits showed 73% diversity in the parameters studied. However, it was...
Aatif Amin1,*, Zakia Latif2, Arslan Sarwar1, Basit Zeshan1 and Mushtaq A. Saleem1
...rDNA ribotyping and phylogenetic analysis were performed for the characterization of isolates as Bacillus sp. AZ-1 (KT270477), B. cereus AZ-2 (KT270478), B. cereus AZ-3 (KT270479) and Enterobacter cloacae ZA-15 (KJ728671). Phylogenetic relationship on the basis of merA nucleotide sequence confirmed 51-100% homology with the corresponding region of the merA gene of already repo...

Ashraf Ismail Afia1 and Ahmed Soliman Mohmed El-Nuby2*

Soybean Genotypes Response to Root-Gall Nematode
...xisted among the soybean genetic resources with regard to the evaluated parameters. According to reduction percentage in reproduction factor (RPRF), the tested genotypes were ranked as resistant or susceptible to challenge nematode genus. The genotypes viz., G22, G111(100% RF) were found to be highly resistant (HR), genotypes ranked as resistant (R) viz., C97, H2, CV1, Clark, A7 (99.97, 99.96, 98.73, 98.29 and 97.06% RF, respectively), moderately resistant (MR...
Muhammad Umer Khan1,2*, Muhammad Farooq Sabar1, Atif Amin Baig3, Arif-un-Nisa Naqvi4 and Muhammad Usman Ghani1
...n adopted as a versatile genetic marker all over the globe and provides a unique maternal ancestry portrait of a person’s genetic pin code. Overall knowledge of mtDNA profiles of worldwide populations can benefit population genetics and forensic sciences. Consequently, this study was designed to establish the mtDNA profiles of the Shin ethnic group in Gilgit-Baltistan, the northern m...

Kecheng Zhu1,2,3, Peiying He1, Baosuo Liu1,2,3, Huayang Guo1,2,3, Nan Zhang1,2,3, Liang Guo1,2,3, Shigui Jiang1,2,3 and Dianchang Zhang1,2,3,* 

...no acids. Moreover, phylogenetic and gene structure analysis indicates that Almyomaker is highly conserved among vertebrates. The tissue distribution pattern shows that Almyomaker is more highly expressed in white muscle than in other tissues. Furthermore, to explore whether two MyoDs are modulators of Almyomaker, a promoter analysis was performed using progressive deletion mutations of Almyomaker. The results of promoter activity assays s...
Gautam Patra1*, Ana Sahara2, Sonjoy Kumar Borthakur1, Parthasarathi Behera3, Subhamoy Ghosh1, Apurba Debbarma4 and Seikh Sahanawaz Alam5
...ed on sequences and phylogenetic analysis of cyt b gene, 8 isolates of P. relictum were identified. The obtained complete nucleotide sequences of cyt b gene from P. relicum revealed100% identity among themselves while the other sequences registered in Gen Bank showed 95% to 99% similarity. In the phylogenetic analysis all the isolates of P. relictum formed a separate clade with high bootstrap valu...
Jianjun Fu1, Shili Liu2, 3, Wenbin Zhu1, Yongyi Jia2, 3, Lanmei Wang1, Yufen Yang4, Zhimin Gu2, 3*and Zaijie Dong1*

Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo

The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
...pment of such strains by genetic manipulation by transferring gene (s) required for saponins production is effective in increasing crop yield together with fixing nitrogen. Escherchia coli is still used as a host for the recombinant DNA technology – based industrial production of proteins and peptides to manage root-knot nematode. Mi gene resistance in tomato plants has been utilized to manage M. incognita and M. javanica. Also, protoplast fusion between...
Anjali Khadka1*, Subodh Raj Pandey2, Subarna Sharma Acharya3, Amrit Poudel4 and Sushma Adhikari1
Morphological Evaluation and Multivariate Analysis of Soybean Glycine max (L.) Merrill Genotypes in Western Mid-Hills of Nepal
...icient of variations and genetic gain, indicating that the heritability was most likely due to additive gene effects and selection may be effective. High heritability for days to maturity, moderate heritability for days to 50% flowering with low genetic advance and genotypic and phenotypic coefficient of variation for both of the above traits were observed. Test weight had a significant positive correlation with grain yield....

Muhammad Ali Shah, Faiz Ur Rehman, Abbas Mehmood, Fareed Ullah, Sayed Irfan Shah and Syed Majid Rasheed*

Combining Ability, Heritability and Gene Action Assessment in Rapeseed (Brassica napus L.) for Yield and Yield Attributes
...and non-additive type of genetic variability indicated that it could be integrated as a valuable tool in breeding strategies to choose genotypes of B. napus L. for high yielding traits. 

...
Jun Yan Bai1*, Ren Tao Di Wu2, Qiang Zhang2, Di Bao2, Le Ma Dao2 and Xing Hua Tian3
...were used to analyze the genetic diversity of Sonid bactrian camels, and the correlation between microsatellite markers and their body size was analyzed to find microsatellite markers associated with the body size traits of Sonid bactrian camels, so as to provide reference for marker-assisted selection of Sonid bactrian camels. The results showed that 64 alleles were detected in 17 microsatellite markers. The average number of alleles was 3.7647, the average h...
Haseeb Nisar1, Usman Pasha1, Kiran Hanif1, Rizwan Abid1Rehan Ahmad Khan Sherwani2, Haja N. Kadarmideen3 and Saima Sadaf1*
...erative to ascertain the genetic contributors of RA in a specific population.
...
Zhilong Tian1, 2, Yuqin Wang2, Jishun Tang1,3 and Mingxing Chu1*
...acid changes. Population genetic analysis indicated that SYNE2 gene g.73310578G>A, g.73312791A>G showed moderate polymorphism (0.25<PIC<0.5) in Small Tail Han sheep. Furthermore, g.73310578G>A and g.73312791A>G loci were closely linked in STH (r2 > 0.33). Association analysis results showed that g.73310578G>A and g.73312791A>G SNPs significantly affected litter size (P < 0.05). In addition, the litter size...
Chaojie Yang1,2,3, Haishan Wang1,2,3, Le Ye1,2,3 and Zhi Chen1,2,3,*
...s the basic step in phylogenetic studies, biodiversity monitoring and conservation. The morphological descriptions and DNA barcoding study about Muraenesocidae in the East China Sea were old, rough and deficient. Morphometric measurements and meristic counts were taken for all collected Muraenesocidae samples in our present study. Teeth characters that are conclusive for the species were consistent with Muraenesox and were sufficient for separation from other ...
Ehsan Kashani1, Hamid Reza Rezaei2*, Morteza Naderi3 and Nematolah Khorasani4

 

...ll;">Knowledge about the genetic diversity of the mammalian species and their plasticity in ever-changing environments is emphasized by scientists and conservation biologists. Central deserts and steppes of Iran host a different kind of insectivores like hedgehogs; however, their ecology and genetic properties are rarely documented. For species of hedgehogs that are included in the subfamily Erinaceinae, at least four known ...

Muhammad Rizwan1*, Jehanzeb Farooq1, Muhammad Farooq1,2, Aqeel Sarwar4, Abid Ali3, Farrukh Ilahi1, Muhammad Asif1 and Ghulam Sarwar1

Quantitative Studies in Upland Cotton (Gossypium hirsutum L.) using Multivariate Techniques
...ere used to evaluate the genetic variability for fifteen parameters viz., plant height (cms), days taken in 50% flowering, monopodia plant-1, sympodia plant-1, boll weight (g), No. of bolls plant-1, ginning out turn (%), lint index(g), seed index(g), 2.5 percent span length(mm), bundle strength(g/tex), micronaire (µg/in), fibre elongation (%), uniformity ratio and yield plant-1(g). Using Mahalanobis D2 analysis, these parameters were assembled into seven...
Maria Syafiqah Ghazali1, Azlan Che’ Amat2 and Nor Azlina Abdul Aziz1*
...nd usually have very low genetic resistance against parasitic infection mainly due to low exposure towards the parasites themselves. However, when herds of these wild animals are kept in captivity, or in zoological gardens, parasitic infections might be worse and pose a serious threat to endangered species. The present study was conducted to observe the occurrence of gastrointestinal parasites in large felines in a Malaysian zoo. Ten faecal samples were collec...
Qianbao Wang, Zhengyang Huang, Shoufeng Li, Huayun Huang, Chunmiao Li and Zhenhua Zhao*
...broiler breeder males of genetically modified F line after sexual maturity. 
...
Sana Mehmood1, Hafiz Muhammad Tahir1*, Muhammad Summer1, Sher Muhammad Sherawat2, Shaukat Ali1*, Sajida Naseem3
...in establishing the phylogenetic relationships among various spider species for diversity analysis in future. Authentic molecular evaluation will also help to correct the misidentified specimens.
...

Tofique Ahmed Bhutto1, Mahmooda Buriro1, Niaz Ahmed Wahocho2, Safdar Ali Wahocho2, Muhammad Iqbal Jakhro3*, Zulfiqar Ali Abbasi1, Reema Vistro1, Fehmeeda Abbasi1, Sanam Kumbhar1, Fateh Muhammad Shawani4 and Nadar Hussain Khokhar5

Evaluation of Wheat Cultivars for Growth and Yield Traits under Agro-Ecological Condition of Tandojam
...ify;">Cultivation of low genetic potential cultivars is one of the main factors which cause yield reduction in wheat. In this regard, the selection of new high yielding cultivars with better adaptability to local conditions is important for growth and yield associated traits. The highly varied performance of crop cultivars of the same species for growth and yield-related attributes is well documented. The present investigation investigated the performance of w...

Saba Aleem1*, Mehvish Tahir2, Iram Sharif3, Muqadas Aleem4, Muhammad Najeebullah2, Ali Nawaz1, Amina Batool1, Muhammad Imran Khan1 and Waheed Arshad1

Principal Component and Cluster Analyses as Tools in the Assessment of Genetic Diversity for Late Season Cauliflower Genotypes
...s measures the amount of genetic diversity in respect of several charaters and asseses the relative contribution of different traits to the total variation. In the present study, nineteen genotypes were evaluated for presence of genetic diversity through principal component and cluster analyses. The experiment was designed in randomized complete block design with two replications at the experimental area of Vegetable Researc...

Muhammad Umair Hassan1, Muhammad Aamer1, Muhammad Nawaz2, Abdul Rehman3, Talha Aslam3, Ubaid Afzal4, Bilal Ahmad Shahzad3, Muhammad Ahsin Ayub5, Faryal Ahmed1, Ma Qiaoying1, Su Qitao1 and Huang Guoqin1*

Agronomic Bio-Fortification of Wheat to Combat Zinc Deficiency in Developing Countries
...to desirable levels. The genetic techniques are costly, and sustainable, however, they are long term and requires large breeding activities and resources. Conversely, agronomic techniques appear to be short term, quick and economic solution to increase the Zn contents to meet human needs. The application of Zn as foliar sprays, seed priming, soil application and soil+foliar application effectively enhanced Zn uptake and grain Zn contents. Zn fertilization also...
Ghulam Sarwar1, Amna Nazir1*, Muhammad Rizwan1, Eram Shahzadi2 and Abid Mahmood3
...ndustry. Awareness about genetic diversity is very essential for fruitful genetic improvement approaches. Correlation, principal component analysis (PCA) and cluster analyses were performed to estimate genetic diversity in 25 cotton genotypes. Correlation analysis revealed that days to first square, flower and boll opening were positively associated with seed cotton yield. Staple length an...

 Shuanping Zhao, Lei Xu, Hai Jin and Yutang Jia*

...an intermediate level of genetic diversity (0.25<PIC<0.5), and SNP g.2213C>G belonged to a low polymorphism level (PIC<0.25). LD (Linkage disequilibrium) analysis showed that SNP g.1694T>A and g.2213C>G had a strong linkage (r2>0.33), a total of four different haplotypes were constructed and the frequency of the main haplotypes AAG accounted for over 61.2 % of the total individuals. Association analysis indicated that all of the...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
...ells that can pass their genetic material to offspring and are also capable of proliferation and differentiation, ensuring the efficiency of passing transgenes from parents to subsequent generations. In this research, to understand the growth characteristics of buffalo SSCs in vitro, methods for their isolation, enrichment, culture and preliminary identification were established. Testes from 3- to 6-month-old buffalos were digested with two-step enzymat...
Mashal Malik and Mudassar Nawaz Khan*
 
...pan> In order to analyze genetic variations, Single sequence repeat (SSR) markers were used. The line B20G16 showed the highest morphological growth parameters of plant height, total number of leaves, leaf width, leaf length and leaf weight, while line B29G11 revealed the lowest. The maximum plant height (128.03 cm) was found for line B20G16. A maximum number of 48 leaves with leaf width of 2.34 cm, leaf length of 3.34 cm leaf and leaf weight of 156.11 mg were...
Muhammad Zubair1, Habib Ahmad2*, Brian E. Hemphill3, Muhammad Tariq4 and Muzafar Shah5
Guofang Wu1,2, Xingxing Xue1, Wenjuan Shen1, Lei Wang1,2*, Yuhong Ma1 and Jiping Zhou1
... of piglets. By applying genetic technology, it may be possible to increase the number of teats and thus enhance production. Previous research has shown that teat number exhibits moderate levels of heritability that could be accelerated by the use of a genetic marker, such as microsatellites. Based on our preliminary sequencing data, the present study describes the use of time-of-flight mass spectrometry (TOF-MS) to identify...

Muhammad Jahanzaib1*, Nazakat Nawaz1, Muhammad Arshad1, Haris Khurshid1, Muzamil Hussain2 and Shahid Ali Khan1

 

 

...as conducted to estimate genetic variability, traits association, and path coefficient analysis in eleven groundnut advanced breeding lines together with check vis BARD-479, using randomized complete block design with three replications at National Agricultural Research Centre, Islamabad, Pakistan. Through One-Way Analysis of variance significant discrepancies were found among the genotypes for most of the traits. In this study 100 kernels weight was fo...
Muhammad Qayash Khan1,2, Muhammad Zubair Anjum2, Muhammad Adnan3, Abbas Khan1, Hafsa Zahid1, Javed Nawab4, Sher Zaman Safi5Muhammad Ishaq Ali Shah6, Atif Kamil7 and Abid Ali1*
...ich not only conceal the genetic diversity but also pose problems in fisheries management and conservation practices. The purpose of the present study was a molecular characterization of fish species of economic importance belonging to the genus Schizothorax, Tor and Mystus collected from various rivers of Khyber Pakhtunkhwa, Pakistan. The samples were morphologically identified, its mitochondrial DNA was extracted and subjected to molecular char...

Natalija Grittner1, Radomir Mandić2,* and Milivoje Urošević3

...is paper presents animal genetic resources of Serbia. Breeds of horses, donkeys, cattle, buffalo, sheep, goats, pigs, chickens, geese, turkeys, ducks, guinea fowls, dogs and bees were analyzed. The number of populations these indigenous breeds in Serbia has been shown in this paper. It is proposed that 15 breeds (13 breeds and 2 strains), which are not protected by the Decree of the Ministry of Agriculture of Serbia on animal genetic
Iftikhar Hussain1, Sardar Azhar Mehmood1, Muzafar Shah2*, Mohammad Salim3, Shabir Ahmed1, Sana Ullah1,Sidra Batool4, Nadia Saeed1 and Khalid Usman1
...th of tail 7.35 mm. Phylogenetic analysis was performed on the bases of CO1 gene sequences. The mean intraspecific divergences of C. sphinx were 0.011%. The nucleotide composition of the sequences showed higher concentration of A+T as compared to G+C. The A+T contents were 53.1% and C+G were 46.8%. In phylogenetic tree the sequences of C. sphinx clustered together with 100% bootstrap suppor...
Rao Muhammad Ramiz1, Arfan Ahmad2*, Aamir Ghafoor2, Muhammad Avais3, Qurat-ul-Ain1 and Muhammad Zahid Iqbal3
...udy reports presence and genetic diversity of BLV in cattle of two districts of Punjab, Pakistan. Blood samples (n=400) taken from local (n=200) and exotic (n=200) breeds of cattle were processed for complete blood count. Based on lymphocyte counts, samples were divided into two groups (A, lymphocytosis, B, normal lymphocytes) and tested through ELISA and nested PCR for serological investigations and molecular characterization respectively. In this study, anim...
Tanveer Hussain, Masroor Ellahi Babar*, Akhtar Ali and Fiaz Hussain
...tigate us to explore its genetic architecture. Pakistan boasts a camel population of about 20 different breeds and a total of one million head. However, till date, scarce genetic data is available on Pakistani camel’s genomics. In efforts to explore the genetics of the camel, we chose to study the Inteferon beta 1 (IFNβ1) gene in in Mareecha camel and non-descript camel breeds i...

Dilara Devranoglu, Ilksen Tavaci, Gonul Filoglu and Ozlem Bulbul*

...s) are the most abundant genetic markers in the human genome. SNPs are used in forensics for predicting the externally visible characteristics of a given individual based on a sample of DNA alone. In this study, we tested degraded DNA samples to determine the eye color prediction accuracy of the IrisPlex system. We used five years old bloodstains and UVC exposed (30 and 60 min) DNA samples. PCR and electrophoresis were performed. The multinomial logistic regre...
Md. Sabbir Ahamed1, Md. Rayhan Chowdhury1, Md. Atik Mas-ud1, Sujat Ahmed2, Md. Shahadat Hossain1 and Mohammad Nurul Matin1
 
 
...ne-height: normal;">Here genetic variability, correlation coefficient and character association of yield and yield contributing traits of 13 local rice cultivars grown in Bangladesh were estimated. Results indicated that most traits including, plant height (PH), panicle length (PaL), leaf length (LL), leaf breath (LB), 100-grain weight (100-GW) as well as filled and unfilled grain per panicle (FGPa, UFGPa), and primary branch (PB) obtained significant variatio...

 Muhammad Ajmal1, Saima Mustafa1, Fizza Ibrahim Bajwa1, Cheng Zhou2, Guangdong Wen2, Soe Lwin Myint2, Syed Irfan Raza3, Ihtasham Bukhari4, Mubashir Hassan5, Muhammad Faisal6 and Furhan Iqbal1*

...tudy was to find out the genetic mutation(s) in hairless (HR) gene, if any, in the enrolled subjects. A questionnaire was filled for each subject on the sampling site in order to collect epidemiological data associated with the disease. Patients from both families exhibited congenital atrichia with papular lesions (APL) including hair loss in the scalp, pubic and other body parts. Polymerase chain reaction (PCR) was used to amplify all the over lapping ...
Umair Riaz1*, Shazia Iqbal2, Muhammad Irfan Sohail2, Tayyaba Samreen2, Muhammad Ashraf1, Fatima Akmal2, Ayesha Siddiqui3, Ijaz Ahmad4, Muhammad Naveed5, Naveed Iqbal Khan5 and Rao Masood Akhter6
...ultivation resources and genetic materials and many more. Therefore, MAPs can be cultivated and adopted to various geographical landscapes if facilitated by governments especially in the rural areas of low-income Asian and African countries.
...
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
...try and wild birds. Phylogenetic analysis of recent Egyptian isolates in Menofia governorate, Egypt was constructed, and showed to be of genotype VII. Eight NDV viruses were isolated from vaccinated commercial broiler flocks showing respiratory manifestation in Menofia governorate, Egypt during 2019. Those viruses showing deaths and haemorages of inoculated SPF ECE eggs, harvested allantoic fluids showed haemagglutination activity by using 1% RBCs and also hae...

Saad A. Moussa1, Ahmed F. Afify1*, Suzan Salah2 and Ayman Hamed3

...ied out followed by phylogenetic analysis. Positive samples were isolated on Vero cell culture and identified by TEM and immune-peroxidase technique. Betacoronavirus 1 was detected by ELISA in 6 out of 20 fecal samples (30%), PCR detected 4 out of 6 ELISA positive samples at specific M.W. band of 236 bp by electrophoresis, and one sample was sequenced and submitted on Genbank with acc.no. MW173144, further phylogenetic seque...
Bram Brahmantiyo1,*, Henny Nuraini2,3, Astari Wibiayu Putri3, Maizirwan Mel4,5 and Cecep Hidayat6 
 

... and had potentiality as genetic resources to develop Indonesian broiler rabbit. The aim of this study was to characterize phenotype of Hycole and Hyla rabbits to evaluate their adaptability in tropical environments. Research were held at Indonesian Research Institute for Animal Production, Ciawi, Bogor, Indonesia using 79 adult rabbits (20 does and 20 bucks of Hycole, 14 does and 12 bucks of Hyla, and five does and eight bucks of New Zealand white). The age o...
Saima Siddiqui1, Ghulam Hussain Abro1, Tajwar Sultana Syed 1Abdul Sattar Buriro2, Sohail Ahmad3, Muhammad Zeeshan Majeed4 and Muhammad Asam Riaz4*

 

...ed on the basis of their genetic characteristics such as nectard (CIM-554, CIM-557and MNH-786), nectariless (Stoneville-701, Stoneville-697, and Stoneville-857), high gossypol (CIM-496 and LAHG 1838-1488) and gossypol free (Greeg-25-V and 3-508 OP), Bt-703 and standard cotton variety (CRIS-342). The incidence of sucking pest on cotton was recorded fortnightly. The physio-morphic characters (density and length of trichome on lamina, midrib and vein, gossypol gl...
Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1
...aragine (N). Further, 64 genetic variants were also identified in STAT5A gene and these variants were due to cattle and buffalo differences. Phylogenetic analysis and evolutionary divergence were also estimated. The sequence was submitted to GenBank (NCBI) with accession number MN712202. Our results represent a preliminary step towards the identification of polymorphisms in STAT5A gene of Nili Ravi Buffalo. Fur...
J.Y. Bai1,*, R.T.D. Wu2,*, Q. Zhang2, D. Bao2, L.M. Dao2 and X.H. Tian3
...were used to analyze the genetic polymorphism, genetic differentiation, gene flow and genetic distance of Alashan desert Bactrian camel, Alashan Gobi Bactrian camel, Sunite Bactrian camel and Qinghai Bactrian camel.The results indicated that the number of effective alleles of the four Bactrian camel varieties ranged from 2.7302 to 3.0524, and average theoretical heterozygosity and average ...
Aiguo Zhou1,2, Di Sun1,2,Shulin Liu 1,2, Yongyong Feng1,2, Yue Zhang3
Yanfeng Chen4, Shaolin Xie1,2* and Jixing Zou1,2*

 

... study, investigation of genetic comparison was performed based on the complete sequences of mtDNA control region for “Bicolor” and “White” types of northern snakehead (Channa argus) due to an uncertain classification of them. The results showed that the genetic distance for the inter-species ranged from 0.000 to 0.492 for the Channidae family. And the analysis of sequences showed that there we...

Fayaz Ahmad, Noorullah Khan, Farrukh Siyar Hamid, Abdul Waheed, M. Abbas Khan, Imtiaz Ahmad, Shamsul Islam, Basharat Hussain Shah, Sohail Aslam and Qamar uz Zaman

... years 2014-16 to assess genetic variability, heritability and genetic advance as percent of mean for various growth attributing traits of fifteen early flushing tea (Camellia sinensis L) clones. The experiment was laid out in randomized complete block design (RCBD) with three replication and 100 plants per replication.  Data were recorded on plant height (PH), number of branches per plant (NBP), number of leaves per pl...

Halil Erhan Eroglu

...romosomal data, two phylogenetic trees were formed. The phylogenetic trees were showed karyotypic relationships among the taxa.
 
Novelty Statement | This is the first report on karyotype asymmetry of Cervidae. Karyotypic relationships are useful to infer processes of evolution and speciation. Karyotype asymmetry is an important parameter that helps to establish phylo...

Hafiz Basheer Ahmad1*, Sajida Habib2, Waqas Shafqat Chattha3, Rizwana Qamar2, Shazia Saeed2, Abdul Khaliq1 and Salsabeel Rauf2

Tanweer Fatah Abro1, Asif Ali Kaloi1, Jay Kumar Sootaher1*, Piar Ali Shar1, Tarique Ahmed Baloch2, Tanveer Ali Soomro1, Muhammad Saleem Chang3, Kirshan Kumar Menghwar1 and Waqar Hussain Shah4

...g 2016-2017 to determine genetic variability, heritability, and genetic gain in nine F4 segregating populations for morphological, yield and fiber quality related traits in upland cotton. Significant differences were observed among genotypes, parents, F4 populations and parents x F4 populations for all the studied attributes which articulated greater genetic variation in the breeding mater...
Yahong Guo1,3, Zeqin Fu1,3, Jiantong Feng1,3, Chengrui Yan1,3, Yingying Ye1,3*, Kaida Xu2 and Baoying Guo1,3
...inbreeding and decreased genetic variation. In this study, four wild specimens groups (ZSW, WZW, NNW, and FZW) and four cultured specimens groups (ZSC, WZC, NDC, and FZC) of M. unguiculatus were used to analyze their genetic diversity, population structure and understand the relationship between the wild and cultured specimens groups. The results showed that haplotype diversity (h) of the cultured specimens gro...
Maria Qibtia1, Muhammad Wasim1, Farzana Chowdhary1, Muhammad Tayyab1, Sehrish Faryal1, Ahmed Mansouri2, Zeeshan Ahmad2, Muhammad Hamid3 and Ali Raza Awan1,*
... management and accurate genetic counseling of the pre-symptomatic diagnosis of LNP. Among the six novel mutations found, mutation XI was found in all of the LNP subjects and was absent in the lactose persistent group. This study, for the first time, focuses on molecular analysis of LCT-gene from Pakistani patients with LNP.
...

Muhammad Tariq Mahmood1*, Muhmmad Akhtar2, Kaiser Latif Cheema2, Mushtaq Ahmad1, Muhammad Jahanzaib Khalid2, Afzal Zahid2, Muhammad Kashif3 and Zeshan Ali4

...ghly imperative for crop genetic improvement programs. The research aimed to determine the yield stability of sixteen advance chickpea strains was carried out at eleven diversified locations across the Punjab province of Pakistan during cropping season 2019-20. GGE biplot and mega environment analysis were employed to identify the most stable and high yielding chickpea advance strains. GGE biplot among PC1 and PC2 illustrated that G-10 (K-15019) was ideal geno...

Arshad Khan1, Mohammad Ihsan1, Mohammad Nisar1, Ali Hazrat1*, Murad Ali3, Rashid Ul-Haq3, Khalid Khan2, Karishma Gul1 and Shah Faisal1 

...in order to estimate the genetic diversity among the barley landraces; to explore significant variation which can be used in breeding programs. For this purpose, 40 barley landraces were collected from Dir Lower and Swat, districts of KP, Pakistan. A total of 18 traits were recorded, 10 qualitative characters including spike density, awn barbs, glume awn, lemma type and awn color, length of the rechilla hairs, lemma color, auricle pigmentation, photoperiod sen...
Zeynep Sonmez1 and Memis Ozdemir2,*
...olymorphic regions is in genetic equilibrium.
...

Ahmed A. Kheder

...h direct sequences. Phylogenetic analysis results indicated that SLRSV-Eg isolate under study (acc. no. MT648777.1), showed 65.9 – 99.5% nucleotide similarity with available homologous sequences from other crops. Reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay which is one of the most promising molecular diagnostic techniques was applied. The amplified products were analyzed using amplification curves and electrophoresis. In ...

Syed Muhammad Sulaiman, Nazir Ahmad and Nazir Ahmad Khan*

...arch study evaluated the genetic variation in forage biomass yield, morphological characteristic, forage nutritional value and digestibility among five selected wheat genotypes. The five cultivars, namely, Pir Sabaq (PS)-15, Bakhtawar-92, PS-2000, National Agriculture Research Council (NARC)-11 and Zam-04 were grown in 12 replicate plots (6 m × 10 m), that were blocked in three fields according to Randomized Complete Block Design. Uniform field preparati...

Muhammad Akhtar1, Muhammad Tariq Mahmood2*, Kaiser Latif Cheema1, Mushtaq Ahmad2, Muhammad Jahanzaib Khalid1, Amir Amin1, Javed Anwar Shah1, Zeeshan Qadeer1 and Zeshan Ali3

... evaluation of available genetic material to evolve drought tolerant chickpea cultivars for drought prone regions of the country. For this purpose, a research experiment was conducted in moisture stress (drought) and non-stress conditions (irrigated) at Gram Breeding Research Station, Kallurkot, Pakistan during 2019-20. Mean performance of various traits revealed significant differences among all the included chickpea advance lines. Drought indices i.e. drough...
Ala’audin Hakami,1AusamaA Yousif,2* Mohamed Zaki,3 Mahmoud Ismail,4,5 Ahmed Al-Ali,5 Abdu-Rahman Al-Ankari5 
...). Nucleotide-based phylogenetic analysis clustered one
African grey parrot PBFDV (Mira08) with South-East Asian and
Australian isolates indicating interspecies transmission.
...
Mansour, L. L.*; Othman, B. A. **; Abd-EL Ghaffar, M. H. **; Eman, M. Marai** and Sahar, A. Youssef* 
...
determine the phylogenetic with other published isolates. Phylogenetic tree showed
the identity percentage of Egyptian isolate group of BBTV-component- 6 with
component 6 of Indian, Taiwanese and Pakistani isolates group was 97%, whereas
it was 84% in case ofChinese isolate group.
...

Mokbel, Samah A.1,Ahmed K. El Attar1; Azza G.Farag2.

... DNA sequencing and phylogenetic analysis of the 16S
rRNA gene fragment from the hibiscus witches' broom phytoplasma showed 100%
homology with the same region of thehibiscus witches' broom strain thatisolatedin Brazil
(PhytoplasmaBrasiliense). The DNA sequence was submitted for the gene bank as the
first report of the Brasiliensephytoplasma associated witches‟ broom affecting hibiscus in
Egypt.

Amal Mahmoud1&2 and Medhat H. Hashem3

...HCC (Sohag1 and 7). Phylogenetic analysis of the nucleotide sequence and the amino acid of the pol or env genes revealed that 7 out of the 8 isolates were closely related to genotype D. Only Sohag 1-HCC isolate was clustered with genotype C which is known as risk factor of HCC. Multiple sequence alignment resulted in unique amino acid substitutions (V320→L and N464→T) and (I284→L, T300→I and K334→R) in pol and env genes respectively. F...
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
...es is high potential for genetic and antigenic variation. Therefore molecular study of FMDV is pre-requisite for amplification of FMDV specific genomic fragment and also copying specific serotypes O, A and SAT2 nucleotide sequence. In this work FMDV serotype O, A and SAT2 were isolated from cattle clinical samples (foot lesions). RNA extracted from clinical samples was subjected to reverse transcriptase polymerase chain reaction( Rt. PCR) nucleotide of FMDV ID...

Yanni, M. I1; Hanaa A. Ahmed2; Lamyaa, A. Ahmed1; Hanan, A. Fahmy3 and Ibrahim, E.M.4 Aggour, M. G.3

...from brain samples. Phylogenetic analysis including sequences from all previous Egyptian isolates and neighboring countries isolates was conducted. The % of nucleotide identities of the Egyptian recent isolate (Egy/cairo /2014) with three previous Egyptian isolates were 98.4-98 and 98.4% respectively. Egy/cairo/14 share nucleotide identity with the vaccinal strain flury LEP and kelev with a percentage of (93.1% and 98% respectively). All Egyptian isolates have...

Fawzy Rania1, AboElkhair M.1, Bazid A.M.1, Sultan H.2, Hussien A.H.3

...y industry. A mixture of genetic mutations and or recombination events has been occurred during replication of coronaviruses including IBV which called quasispecies. Serotypic and or genotypic classification of IBV is mainly based on the S1 subunit of Spike (S) gene. In the present study, thirty tracheal samples of broiler flocks suspected to be infected with IBV were collected from different Egyptian governorates during 2012. Isolation and

Aya El-Turkey1, A. K. El-Attar1, A. E. Aboulata1, B. Othman2 and K. A. El-dougdoug2

... regeneration medium and genetically transformed with the HSV-2gD-PB21 binary vector through Agrobacterium co cultivation. Agro-inoculated tomato plants were tested for the HSV-2gD insertion and transcription using both PCR and RT-PCR and the results were successful in getting insert-containing tomatoes against HSV-2gD. Specific expression of the introduced antigen construct at the RNA level was detected by RT-PCR, using specific primers for the HSV-gD2 transc...
Amro A. Farrag1; Ahmed K. El-Attar1; Om-Hashem M. El-Banna2; Ibrahim A. I.2; H.
M. Mazyad1
...in the GenBank. The phylogenetic analysis of the complete
nucleotide sequence revealed that SLCV is related to other isolates of SLCV from other
governorates in Egypt (Cairo and Ismailia) as well as isolates from Lebanon (SLCV-LB2),
Palestine (SLCV-Pal), Jordan (SLCV-JO) and Israel (SLCV-IL) sharing high identities
ranging from (90% to 97%).
...

Hanaa H. A. Gomaa1; Entsar A. A. Nassar2; K. A. El-Dougdoug3

...iseases. All the studied genetic parameters showed variations among milk thistle and parent varieties (plant height, main branch, total branches, head flower, fruit yield per plant). Concentration and yield amount of seven detected silymarin compounds using HPLC showed wide variations among varieties. They ranged from 9.2 to 34.78 mg/g fruits or 450.25 to 1250.25 mg/plant. The silymarin production has the same pattern of fruits yield. The methanolic solutions ...

Ahmed K. El-Attar; Samah A. Mokbel; Ali H. Hamed

...uence alignment and phylogenetic analysis showed that the
Egyptian isolate of the OYDV is related to other isolates from Japan, Netherlands, Israel,
Brazil, China, India, and Poland with a similarity ranged from 70 to 93% among those
isolates available on GenBank. The virus was eliminated from infected onion plants using
cytokinin (kinetin) in a tissue culture line to obtain the OYDV-free plantlets. The effects

K.A. El-Dougdoug1, A.R. Sofy2, A.A. Mousa2 and E.E. Refaey2

...tered in GenBank. A phylogenetic tree of Ryadg gene based on nucleotide sequence
revealed 93% a moderate degree of similarity to Potato gene Y-1 isolated from Solanum
tuberosum subsp. andigenum
...
Amro A. Farrag1; Ahmed K. El-Attar1; Om-Hashem M. El-Banna2; Ibrahim A. I.2; H. M. Mazyad 1 
...uence alignment and phylogenetic
analysis showed a relatively high diversity among the three different isolates that the
identity ranged from 89 to 94%.
...
Aly M. Abdel-Salam1, Rehab A. Dawoud2, Amira M.E.Aly2, and Salama M. El-Saghir2
...d from the GenBank. Phylogenetic analysis based on the partial RT/RNaseHsequence
showed that the four Egyptian BSV isolates clustered together with two
Egyptian sugarcane bacilliform badnavirus apart from other badnaviruses
circumventing several viruses of BSV.
...

Aly M. Abdel-Salam

...ty range of 66-91%. Phylogenetic analysis based on amino acid
sequence identity indicated the clustering of the two SCBV in a separate branch from
the other known SCBV; indicating the special nature of the Egyptian SCBV isolates.
Close serologic relationship between SCBV and BSV from Egypt was detected. The
Egyptian antiserum of BSV captured SCBV antigens in leaves and insect vectors in
Immunocapture ...

Amira A. Mazyad1; A. A. Kheder1; Ahmed K. El-Attar1; W. Amer.2; Mahasen, H. Ismail.2; Amal, A.F.1

... DNA sequencing and phylogenetic analysis for the coat protein gene. Further insurance of SLRSV infection was performed using light microscopy which showed presence of amorphous inclusion bodies, electron microscopy and chemical analysis.

...
Eman A. Ahmed1, Osama Y. Shalaby2, Emad F. Dwidar2, Samah A. Mokbel1, Ahmed K. El-Attar1 
...The DNA sequencing, phylogenetic analysis and the
multiple alignments for the sequences of the Egyptian clones with each other and with the
other sequences of phytoplasma strains on GenBank showed that we may have two
different phytoplasma isolates infecting tomato plants in Egypt.
...

Sherif .M. Ibrahim*, Abd El-Razek. B. Abd El-Razek*. Hanan. M. El-Zahed Amal. A. Fatouh* and Ayatollah. I. Ibrahim*

...nd vaccine strains. Phylogenetic analysis for the amplified P4p product revealed close similarity of the FPV isolate (FPLH) and fowl pox vaccine (FPVV) with the published sequences for FPV in the Genebank. Unexpectedly, PPV isolates (PPLA, PPLR) and PPV vaccine (PPVV) showed high similarity to the published FPVs-P4b sequence in gene bank and distributed under genogroup A1 in the phylogenetic tree, while the PPV isolate (PPLH...

A. A. Rezk1,3, K. A. Alhudaib2 and A. M. Soliman2,3

...umber JN083790. The phylogenetic tree showed that there are two big clusters and the identity between them 90%. The isolated CYSDV in this study is located in the second cluster with the isolates from Sudan, Iran and the other isolate from Saudi Arabia. The analysis showed that the highest nucleotide identities were 100% with other isolates that isolated from Saudi Arabia and was 98% with other isolates from Iran and Sudan. While the identity was 90% with all ...

Amany Adel1, Abdelsatar Arafa1, Hussein A. Hussein2 and Ahmed El-Sanousi 2

...on the basis of the phylogenetic analysis of the HA gene sequences to study the genetic evolution of the NS1 gene of such viruses. The viruses were isolated from chickens and ducks in both live bird markets and backyard populations. NS1 gene was amplified, sequenced and the phylogenetic analysis of the obtained sequences revealed deletion of 5 amino acids at positions 80 to 84 in the NS1 p...

Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1

...id (N) gene with further genetic and antigenic characterization. Methods: Beside a real time RT-PCR; sequence analysis of partial S1 gene, cross-neutralization and the challenge experiment for H120 vaccinated broiler chickens were also performed. Results: Egypt/Qal/014p had close relation with that of the Massachusetts prototypes. Comparisons nucleotide and amino acid sequence of partial S1 gene showed that the recent isolate had 98.2% and 95.2% nucleotide sim...
Muhammad G. Khodary1, Ayman H. El-Deeb2, Mohamed M. Emara2, Othman E. Othman1, Hussein A. Hussein2
...CR). Sequencing and phylogenetic analysis of 6
representative samples were performed.
Results: Testing of 80 collected samples of Oropharyngeal, oral epithelial tissue and/or vesicular
fluids by real time Reverse transcriptase polymerase chain reaction (rRT-PCR) revealed 65 out of 80
FMDV positive samples (81%).Typing of the highly viral loaded FMDV positive samples (40 out of
65) by conventional reve...

Naglaa F.S. Awad1, Gamelat K.F. Kotb2

...udy aimed to investigate genetic characterization of recent RHDV isolates from
naturally-infected rabbits in Sharkia governorate, Egypt in 2015/2016.
Methods: A total of 24 clinical samples were investigated by real-time reverse transcription PCR
(RRT-PCR). Hemagglutination analysis of RRT-PCR positive samples were performed, also these
positive RRT-PCR samples were purified and directly partial sequenced for C-t...
Rasha M. Mahrous1,4, Ahmed K. El-Attar2, Ahlam A. AlWatban1, Sohair I. EL-Afifi3,
Nagwa M. Aref1,3
...uo;KJ948653”. Phylogenetic analysis showed a high similarity (99%) with Oman isolate.
Conclusion: Phytoplasma strain of LWB-Eg Candidatus Phytoplasma that reported as a natural causal
agent from Lime WB strain in Egypt was classified as 16SrII subgroup.
...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad
...nce and
phylogenetic analysis of the RPO30 gene and open reading frame (ORF) 103 genes. The sequences
designated Sheep pox virus was submitted to GenBank under accession number of MF443334.
Conclusion: The molecular techniques based on the RPO30 and ORF 103 genes are efficient for the
characterization and differentiation of Capripox viruses. This finding may provide new information on
the epidemiology...

Dina A. Abdulrahman1, Ayman H. EL-Deeb2, Momtaz A. Shaheen1 & Hussein A. Hussein2

... were sequenced for phylogenetic analysis.
Results: FMDV RNA was detected in eight out of the 12 samples tested using real time RT-PCR.
Conventional PCR revealed that Six of the eight samples were positive using pan FMD primers.
However, using serotype-specific primers; only four samples tested positive; two samples for serotype
O, one sample for serotype A and one sample for serotype SAT2 while two samples remai...
Dalia M. Omar1, Nermeen A. Marden1, Lamiaa M. Gaafar1, Elham A. El-ebiary1, Khalid El-Dougdoug2, Badawi Othman2, Abd Elsattar Arafa3, Hussein A. Hussein4
...isera for H5 antigen and genetically by RTPCR
using specific primer for H5 gene and it was confirmed to be H5N1 and grouped according to the
year of isolation as 2016 isolate strain.
Results: According to the phylogenetic analysis it falls into the classical group of Egyptian AI viruses
subclade 2.2.1.2 which is dominant in Egypt since 2012 till now. Therefore, the virus was id...
Ruqayya Bint Khalid1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Zubair Shabbir3 and Masroor Ellahi Babar4
...l were utilized for phylogenetic tree construction and statistical analysis, respectively. Characterization of physical and chemical properties of β-casein protein was performed by ProtParam and SWISS MODEL was utilized for protein model prediction. Sequencing results of amplified PCR products revealed total 9 SNPs including 1 in exonic and 8 in intronic regions of β-casein gene. Results represent the presence of A2 allele in milk of Cholistani Cattl...
Abdullah Sheikh1,*, Faisal Almathen1,2 and Hairul Islam Mohamed Ibrahim3,4
...upportive in genotyping, genetic selection, breeding and classification.
...
Saira Gul1,2, Sohail Ahmed2*, Tahir Usman1*, Khalid Khan3, Sultan Ayaz1, Saleha Gul4 and Nawab Ali5
... different origins. Phylogenetic analysis of local isolates showed a close homology with the isolates from Australia, Brazil, Turkey and Japan. It was found that aged, exotic and crossbreed cattle were more susceptible to A. marginale infection in summer season compared to the younger and indigenous cattle breeds, respectively (P˂0.01). Moreover, the study revealed widespread prevalence of A. marginale in the subt...

Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2

...nucleotide sequences and genetic characterization was conducted on (G-PCR)
gene segment of these isolates.
Results: 93 samples were positive for LSDV by using conventional PCR direct from nodular lesion, 4
positive samples were inoculated on CAM showed characteristic pock lesion, formerly after passaging
of samples on MDBK cell line revealed characteristic cytopathic effect of LSDV, as well as tissue
...
Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2
...d by sequencing and phylogenetic analysis.
Results: The study revealed that 19% of our samples were positive to EHV-1 and phylogenetic
analysis showed that the Egyptian EHV-1 isolates were more than 99% similarity to European
abortogenic isolates (EHV-1 strains: Army 183 and Suffolk/48/2013). Indeed, the analysis reports that
these viruses are circulating in both Arabian and fo...
Othman N. O. Mansour 1, Naglaa Hagag 2, Momtaz A. Shahein 1, Sayed S. Hassan 1 Ahmed
A. EL-Sanousi 3 and Mohamed A. Shalaby3
...pecific antibodies. Phylogenetic analysis of coronavirus ORF1a partial sequences in some positive
nasal swab samples of imported breeds (with accession number on gen bank; MH184776, MH184776
and MH184777) confirmed presence of different branch of MERS-CoV from which isolated in Middle
East.
Conclusion: MERS-CoV is amphixnoses in which both human and camels are source of infection and
cause mild to sev...

Ayman S. El-Habbaa1 and Mervat E. Radwan2

...ate strain detection and genetic analysis of BEF virus among suspected cattle in
August 2017, Qualyubia, Egypt.
Methods: Partial sequence was generated after detection by reverse transcription-polymerase chain
reaction (RT-PCR) and subsequent gel purification of the amplified products of G gene of BEF virus.
Results: BEF virus was circulating in this region. Sequence analysis of G-gene of this Egyptian strain,

Ahmed K. El-Attar1, Samah A. Mokbel1 and Om-Hashem M. EL-Banna2

...ion and
phylogenetic analysis were performed for the AMV coat protein (CP) gene. An amplicon of the
predicted size (∼666 bp) derived from O. basilicum isolate was purified and cloned in E.Coli
into pCR®4-TOPO vector before proceeding to DNA sequencing and the alignment of
sequences.
Results: Electron microscope examination of negatively stained preparations from
symptomatic basil l...

Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer and Ahmed A. El-Sanousi

...21-E03/2018 (H9N2). Phylogenetic analysis of the obtained sequence
cluster the characterized strain along with (formerly) G1-like viruses’ subtype B like those circulating
in the Middle East.
Conclusion: the AI H9N2 avian influenza virus is circulating causing major problem among chickens.
More studies need to be addressed to understand the real effect of the H9 virus on chickens and
humans in L...

Aml S. El-Saadany1, Ayman H. El-Deeb2 and Hussien A. Hussien2

...vernorates. Also, a phylogenetic analysis based on the sequence of the hyper variable
region of VP2 gene was carried out.
Methods: Forty field bursa samples were collected from different localities. IBDV was detected using
RT-PCR. Sequence analysis of the variable region of VP2 gene purified from the PCR product.
Comparative phylogenetic analysis based on the sequence of the hy...

Shimaa M. Gad1, Ahmed A. Kheder1, Mohamed A. Awad 2

...6R2. Sequencing and phylogenetic analysis were performed to identify the
detected phytoplasmas.
Results: Phytoplasma was transmitted successfully from naturally infected gazania to healthy
ornamental plants by dodder and insect. Transmission electron microscopy (TEM) revealed that,
phytoplasma-like bodies were detected inside phloem, sieve tubes and parenchyma cells of leaf
midribs tissues in infected...

Ahmed M. Soliman

...tes of CMV suggesting no genetic diversity. The four CMV isolates located in one cluster, belonging to subgroup IA.
...
Huda S. Darwish 1, Om-hashem M. El-Banna2, Mohamed S. Abbas3, Hoda M. Waziri 1, Maisa A. Awad1
...Indicting changes in the genetic make-up of the treated
tomato plants. No similar fragments were observed in the control samples.
Conclusion: NaN3 at 3mM concentration and EMS at 0.4% concentration gave the highest effect in
resistance to ToRSV on tomato and revealed in different unique fragments in ISSR PCR reaction,
without harmful effect on the produced plants.
...
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
...o directly represent the genetic content of entire communities of organisms in the
given harvest.
Results: In this study a 3rd generation sequencing (TGS) was applied using a Nanopore technology
(MinION) based on identification of DNA bases by measuring the changes in electrical conductivity
generated as DNA strands pass through a biological pore (nanopore). Its portability, affordability, and
speed i...
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2
...even decades old and are genetically distant from the currently circulating virulent
Newcastle disease virus (NDV).
Objectives: In this study we compared two vaccination programs against NDV in chickens,
one using genotype VII inactivated and live NDV vaccine are available in the market and
second group used genotype II inactivated LaSota vaccine with live vaccine.
Method: NDV isolate designated NDV-G...

Samah A. Mokbel, Eman A. Ahmed, Hanan F. El-Kammar, Ahmed A. Kheder

...div>
Results: Phylogenetic analysis results indicated that SEA-CMV-isolate under study (acc. no.
MT491996) closely related (99.9%) to Beet-EG-CMV-isolate (JX826591), isolated from sugar beet
plants from Kafr El-Sheikh governorate, Egypt during 2012. Results of sequence analysis confirmed
the classification of isolate SEA as a member of group IA. Infection of sugar beet leaves with CMV
resulted in the format...

Zainab Iftikhar, Muhammad Arif, Iqbal Munir and Sajid Ali

...asis on novel sources of genetic variation, including hybrid wheat. The present work was designed to assess the potential of Chinese wheat hybrid germplasm for cultivation across Pakistan. For this purpose, 416 hybrids were assessed for diversity in crop duration parameters in the year 2017-18 and a subset of better performing 108 hybrids were selected and assessed for subsequent assessment. During the first experiment of preliminary testing of 416 Chinese whe...
Song Jiang1,2,3, Xianbin Mo1,2, Falin Zhou2,3, Jianhua Huang2,3, Qibin Yang2,3, Lishi Yang2,3 and Shigui Jiang2,3*
...r 56 days to analyze the genetic parameters of body weight and survival traits, as well as the interaction effect of genotype and environment. The results showed that the average body weight and survival rate of P. monodon in Diet A group were higher than those of Diet B group, and the heritability of P. monodon in Diet A and Diet B group was 0.53±0.12 and 0.39±0.09, respectively, which belonged to high heritability, and the heritab...

 Yongmin Li1,2, Huabin Zhang1, Xiaoyou Wu1, Dongwei Li 2, Peng Yan1 and Xiaobing Wu1*

...d species to assess phylogenetic relationships of Ranoidea. Phylogenetic analyses using maximum likelihood (ML) and Bayesian inference (BI) methods supported the sister-group relationship between ((Rhacophoridae + Mantellidae) + Ranidae) and Dicroglossidae. Within Rhacophoridae, two species of the genus Rhacophorus (R. schlegelii and R. dennysi) were clustered together with the representative of the genu...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
...the hormone analysis and genetic determination of SRY gene as some of infertile human had smaller sized testis. Blood and semen samples were collected from clinically diagnosed 130 oligozoospermic males throughout D. I. Khan. The patients included had been married for 3 to 19 years, their wives had normal reproductive capabilities; the couples were living together for more than 3 years but fertilization had not occurred. Separation of sperm samples from...

Jie Gao1, Guannan Wang1, Chuang Zhou1, Megan Price2, Jinnan Ma1, Xiaohong Sun1, Benping Chen3, Xiuyue Zhang2 and Bisong Yue1*

...s been considerable phylogenetic research on babblers. Therefore, we aimed to determine F. cinereiceps’ mitogenome and investigate its phylogenetic relationships within the babblers and superfamily Sylvioidea. F. cinereiceps’ mitogenome is typically circular, 16,969 bp in size with a rich A+T content (52.7%), 13 protein-coding genes (PCGs), 22 tRNAs genes, 2 rRNA genes, a control region (CR) and a n...
Nianhong Huang, Yan Wu, Yuanyuan Li* and Jinhong Zhao*
... and the variation. Phylogenetic analysis based on the 18S rRNA gene analyzed in the present study together with other published Hepatozoon species showed that there is no significant difference in the geographical distribution and limited host specificity. This is the first time to report the infection of Hepatozoon sp. in Naja atra for the endemic species in Chinaand increases the number of known host species. Morphological and mo...
Arshad Khan1, Mohammad Ihsan1, Ali Hazrat1*, Mohammad Nisar1, Muhammad Laiq1, Maryam Bibi1, Nasir Ali2, Ulfat Naz1, Muhammad Zakria1, Nausheen Nazir3, Adam Khan4 and Muhammad Asif Nawaz5
...ere found with the total genetic diversity of (0.68%) found  in band 1, while in band 2, the variation was 0.63%. A cluster dendogram was constructed for total seed storage proteins and divided 7 sub clusters, where CA1 and CA69 were found the most diverse genotypes. The main aim of this study was to explore morphological variation in order to generate data that could assist in determining the most appropriate tools that are adaptable to various environme...

Sajid Abdullah1*, Huma Naz2*, Zunera Abbas1, Uzma Nazir1, Mehrunisa Basharat1, Tanveer Ahmed3, Adnan Ahmad Qazi2

...se in heavy metals cause genetic damage in exposed species like fish. Therefore, this study focused on the genotoxic effects of lead (Pb), chromium (Cr) and cadmium (Cd) on nuclear abnormalities in peripheral erythrocytes of Wallago attu were assessed by micronucleus assay. Fish were exposed to different concentrations (1/3rd, 1/4th, 1/5thand 1/7th of LC50) of Pb, Cr and Cd, separately, for three weeks. Blood sample of fish was collected to see the micronuclei...
Sen Liu1,2, Yue Zhu2, Haixia Leng2, Ying Wang1, Xiangju Yin1 and Yajun Zhao1*
... In previous studies, epigenetic variation in the muscles of bat populations were explored; however, the extent of the patterns and levels of genomic DNA methylation among tissues in chiropteran animals have yet to be investigated. In this study, the epigenetic variation of six tissues in normal state Rhinolophus ferrumequinum (i.e., no pregnancy, nursing, or hibernation) was explored using the F-MSAP technique, inclu...
Hui-Ming Li1, Bi-Ze Yang1, Xue-Jun Jin1,2, Lin-Miao Li1, Hai-Ying Jiang1
Xiu-Juan Zhang1 and Jin-Ping Chen1*
...ter understanding of its genetic diversity and structure. In this study, 24 novel SSRs were isolated from full-length transcriptome and they were used for assessing of parent-offspring relationship for M. javanica. All SSR markers were highly polymorphic with a mean of 6 alleles per locus, ranged from 2 to 10 alleles. The average polymorphism information content (PIC) was 0.62. The observed (HO) and expected average heterozygosity (...
Chaochao Hu1,2, Xue Xu1, Wenjia Yao1, Wei Liu3, Deyun Tai1, Wan Chen4 and Qing Chang1*
...ng further study of phylogenetic and evolutionary analysis for wader, and improves our understanding of the evolutionary and taxonomic research within Charadriiformes.
...
Zhongyong Wu1, Wenzhu Li2, Chunxia Ni1, Zhuolin He1, Xiao Hou1 and Wanxia Pu1*
...titis, to understand the genetic polymorphisms of dairy-sourced Staphylococcus aureus, we conducted genotyping of Staphylococcus aureus isolates from milk samples collected from cows with clinical mastitis using the random amplified polymorphic DNA (RAPD) method with three oligonucleotide primers. In total, 177 Staphylococcus aureus isolates were obtained from dairy farms in 5 different provinces in China, including Guizhou, Inner Mongolia...

Huda Bilal1, Hasnain Raza2*, Kaynat Ahmed2, Iqra Tariq2, Qurat-ul-Ain2, Sana Sarfaraz1, Sanaullah1, Maryam Maqsood3 and Ali Raza3

...iversity at the habitat, genetic, and species levels in a synergistic way. Land and aquatic biodiversity greatly influenced, some species are exhibiting different responses to these changing climatic factors like behavioral, morphological, phenological, reproductive, and genetic changes. After all these responses there are many species that are endangered and extinct. This review illustrates that climate change is a severe e...

Muhammad Nauman1, Iftikhar Ali3, Nazir Ahmad1,2*, Fazli Ahad1 and Touheed Iqbal4

... study aimed to estimate genetic variability, heritability, and genetic advance for quality characters in Brassica carinata L. A total of 22 genotypes comprised of six parental lines and their 16 bulked F2 populations were evaluated in a randomized complete block design with three replications at The University of Agriculture Peshawar during 2013-14. Data were recorded on oil content, protein content, oleic acid, glucosinola...

Amir Muhammad Khan1, Laila Fayyaz2*, Raziuddin2, Sajid Ali1, Israr-ud-Din1, Sheraz Ahmad2, Haidar Ali1 and Ijaz Ahmad2

...esults of current study, genetic variation was observed in studied lines. Also, CA-2 and CA-5 were identified as superior genotypes for seed yield and hence were the best genetic resources to increase seed yield in rapeseed. Traits including primary branches on main raceme, pods mainraceme-1, pod length and erucic acid were important while making indirect selection to improve seed weight and oil content in rapeseed. 
Dong-Min Hou1, Ting Jia2, Chun-yan Liu1, Zheng-Kun Wang1 and Wan-Long Zhu1*
... 16 (PRDM16), bone morphogenetic proteins 7 (BMP7), peroxisome proliferator-activated receptor α (PPARα), cyclooxygenase 2 (COX-2) and peroxisome proliferator-activated receptor coactivator 1α (PGC-1α) of WAT were measured. The results showed that body mass, food intake and RMR were decreased in T. belangeri under FR condition, and the relative expressions of COX-2 and BMP7 in WAT were also declined. After refeeding, the above in...
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...published report on phylogenetic analysis of Eimeria tenella. In this retrospective study, tissue samples were collected from a flock of clinically infected chicken followed by haematology, serum biochemistry, and histopathology. Species specific PCR based on polymorphic site of the ITS1 gene was developed and used to identify the organism. Haematological examination of the blood demonstrated a decrease in total erythrocytes, packed cell volume, ...

 Song Jiang1,2, Xianbin Mo1,2, Falin Zhou2,3, Jianhua Huang2,3, Qibin Yang2,3, Lishi Yang2,3 and Shigui Jiang2,3*

...urvival is available and genetic materials for breeding selection are demanded in future.

...

Mohammad Aquil Siddiqui1*, Muhammad Mahran Aslam1, Mahboob Ali Sial1, Nighat Seema Soomro1, Muhammad Tahir Khan1, Shafquat Yasmeen1, Shumaila Sial2 and Imtiaz Ahmad Khan1

Ziyue Qin1, Zhen Wang1, Ali Mujtaba Shah3,4, Zifan Ning1, Yaofu Tian1, Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Lin Ye1, Diyan Li1, Gang Shu2 and Xiaoling Zhao1,*
... chickens with different genetic background have the same response to feed restriction is not reported, herein we evaluated three levels of feed restriction effects on growth performance and blood glucose concentrations in faster- and slower- growing chicken (different genotypes). In the present study, birds were restricted to 100% to full feed in faster- and slower- growing broilers groups. Body weight gain, feed consumption, blood glucose concentrations were...
Zhi-Peng Li, Laiba Shafique, Saif ur Rehman, Kui-Qing Cui, Xiao-Can Lei, Xing-Rong Lu, De-Shun Shi* and Qing-You Liu*
...isappeared after parthenogenetic activation, re-appeared and elevated in the blastocysts stage. The following western blot analysis revealed a similar expression pattern with QRT-PCR and immunofluorescence. In conclusion, the expression pattern of Gas6 gene is closely related to oocytes maturation and embryo development. Gas6 may play a crucial role in the process of oocytes maturation and improve the development potential of early embryos by promoting ...
Manar M. Farouk1*, Amal El-Molla1, Fayez A. Salib1 and Yousef A. Soliman2
...ins, and 3) Build a phylogenetic tree for the partial codon sequence of stn gene of some recovered strains in order to provide a scientific basis for the implementation of practical preventive measures. A total number of 518 diarrheic sheep and goats belonging to 7 mixed flocks of sheep and goats were enrolled and from which rectal swabs were collected and subjected to culture for Salmonella spp. Enterotoxin (stn) gene was detected using p...
Shoaib ur Rehman1, Jabbar Khan2*, Raaza Malja Khan3, Maimoona Azam4 and Zeeshan Mutahir5
...renatal diagnosis (PND), genetic counseling and carrier screening for controlling the affected births in the population. Consanguinity, lack of awareness and non-availability of health facilities have thus contributed to the high occurrence of β-thal and miserable life of the patients.
...

Iffat Nawaz1*, Tahseen Zeb1, Bibi Saima Zeb2 and Javaria Sherani3

...und highly heritable and genetically controlled as no environmental influence was observed based on two years data. Therefore, these traits can be considered to identify and distinguish landraces from each other, may be used as morphological markers in maintaining germplasm purity. 

...
He Yujiao1,2, Xie Huichun1, Lin Gonghua2,3, ZhangTongzuo2,3, Su Jianping2,3 and Du Yurong1*
...ssment of the population genetic structure.
...
Khanitta Ruangwittayanusorn1, Doungnapa Promket1*, Kamonnate Pimrueng1, Jennarong Kammongkun2
...s could be the potential genetic markers for chickens’ selection to improve egg number.
 
Jarosław Pytlewski1, Ireneusz R. Antkowiak1 and Ewa Czerniawska-Piątkowska2*
...e determined between the genetic variants of the three polymorphic sites of the PIT-1 gene and the following rearing parameters: body weight at birth, body weight at weaning on day 270, daily weight gain from birth to weaning, age at first calving and body weight after first calving. The frequency of alleles of the analysed loci was: T =0.2887 and G =0.7913 (IVS4-39G>T), A =0.1087 and G =0.8913 (...
Nabila Rasheed1*, Bushra Waseem1, Kanwal Haneef2, Shumaila Usman3, Madeeha Sadiq1, Tabinda Urooj1, Sarah Tarique2 and Dabeeran Zeha4
...not only responsible for genetic problems and certain disease risk factors in adult life but also have adverse effects on perinatal environment. This study was designed for the comparative analysis of viability and healing properties of GDM affected WJMSCs (GDM-WJMSCs) with Normal controls (N- WJMSCs). Mesenchymal stromal cells were isolated from Wharton’s jelly of human umbilical cords from normal and gestational diabetic mothers. Cell viability was tes...
Xiaoyun He1, Yongfu La2, Jinxin Wang1, Ran Di1, Qiuyue Liu1, Xiangyu Wang1, Wenping Hu1 and Mingxing Chu1*

...es evidence in sheep for genetic markers that might be used in breeding program. 
...
Samaria Nisar1, Tariq Manzoor Khan1, Muhammad Ahsan Iqbal1, Rahmat Ullah2, Muhammad Asim Bhutta3*, Saghir Ahmad3, Amna Bibi3, Hafeez ur Rehman4, Umar Farooq4 and Muhammad Zubair Ishaq5
Aiguo Zhou1,2,4, Shaolin Xie1,2, Zhuolin Sun1,2, Yongyong Feng1,2, Shulin Liu1,2, Di Sun1,2, Yanfeng Chen3 and Jixing Zou1,2*

Rehman Shahzad1, Saba Irshad1* and Faisal Amin2

...utational analysis. Phylogenetic analysis revealed that B2 sub-lineage is endemic in the country as all isolated H9N2 strains belongs to this clade of G-1 lineage. Two isolated H9N2 strains A/Chick/Pak/303/2018 and A/Chick/Pak/436/2019 contain six glycosylation sites instead of seven as found in their ancestral strains. A comparative analysis of neuraminidase amino-acids sequences of B2 sub-lineage with Y- 280 lineage and B1 sub-lineage demonstrated that there...

Asmaa G. Mubarak1*, Mona M. Mustafa2, Mohamed W. Abdel-Azeem3, Dina N. Ali2 

...ene through which a phylogenetic analysis was conducted for six isolates showing a high degree of similarity between them and those imported from Genbank. hilA, spvC, stn, and qacED1 genes were detected in 75, 16.67, 66.67, and 50% of Salmonella serotypes, respectively. These findings signify the role played by chicken-ready meals, as well as their handling, in the high rate of multidrug-resistant Salmonella isolates and the risks it poses to public health.

Rehman Shahzad1, Saba Irshad1*, Malik Saddique Mehmood1 and Faisal Amin2

...this viral segment. Phylogenetic tree analysis showed sequences from 2015 to 2017 form a single evolving clade. Valdar residues conservation scores by multiple sequence alignment showed the C terminal region of NEP protein is conserved while C terminal region of effector domain (ED) of NSI protein exhibit mutations. These mutations are enhancing the total hydrophobicity of the molecules. Hydrophobicity was calculated by using Kyte and Doolittle method. High hy...

Kusuma Adhianto*, Satria Ibnu Lenanto, Akhmad Dakhlan, Muhamad Dima Iqbal Hamdani 

...to find out the value of genetic and phenotypic correlation between weaning weight and one yearling weight of female Saburai goats in Sumberejo District, Tanggamus Regency. A total of 100 female of Saburai goats aged one year were used in this study. This research data uses primary and secondary data taken from direct observations in the field and livestock recording data from Sumberejo District. The method used is a survey method, the research sample was dete...

Mary A. Sargious1, Hanaa A. Ahmed1, Ragab M. El-Shawarby2, Hatem H. Bakery2, Sherif I. Ramadan3, E. Gus Cothran4 and Ayman Samir Farid5*

...he current status of the genetic diversity and inbreeding of Arabian horse populations reared in Egypt. Secondly, to examine the traditional maternal based strain classification system “Al Khamsa” using samples of native Arabian horses reared in the El-Zahraa stud based on 16 microsatellite markers. El-Zahraa stud showed high inbreeding (FIS = 0.110) and should be corrected by modifying mating system through avoiding excessive use of certain sires ...

I Nyoman Sulabda1*, I Nengah Wandia2, I Ketut Puja3 

...tle showed low level of genetic diversity. This result would be used as an initial guide for conservation decisions in future for survival of this valuable genetic resource. The alleles identified from the present study should be further confirmed by sequencing.

Keywords | Taro white cattle, Microsatellite, BoLA, Allel, Heterozygosity 

...

Jiao Ma*

...tracellular matrixes and genetic factors. In general, it is well known that these variables could have an appropriate role to improve the effectiveness of the treatment of heart lesions. The work of cytokines and growth factors has been found to play an important role in increasing endogenous cardiac stem cell proliferation and migration capacity. One of the successful approaches is to use these factors along with cardiac stem cells to boost the repair efficie...

Abdul Haleem1*, Ghulam Hassan1, Arshad Iqbal2, Fahim Ullah Khan3, Muhammad Sajid3, Farhad Ahmad4, Rafi Ullah Khan5 and Mohammad Ilyas2

...ly generation. To assess genetic variability, broad sense heritability and traits varied from, 36 lines of wheat were field tested in RCBD with three replications. The experimental materials include twenty eight F2 population and eight parents. Data showed considerable genetic variation amongst the studied lines, parents, F2 progeniessand parent versus F2 progenies for all the studied traits. Among parents, best lines were A...

Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3 

...tion is a core aetiopathogenetic factor in the development of leukaemia in cats. The fundamental methods for such pathologies diagnostics, along with haematological analysis, include the replicative and expressive activity of FeLV evaluation in the infected organism cells, innate immune factors for antivirals evaluation, interferon system (IFN) in particular, molecular guard factors for cell genome integrity evaluation (p53, etc.). Methods. In domestic cats wi...

Javed Khan1, Abdul Majid1, Mohammad Nisar2*, Ali Hazrat2, Nausheen Nazir3, Muhammad Zahoor3, Mohammad Ihsan2, Azhar Hussain Shah1, Muhamad Ajmal Khan4 and Muhammad Yahya1

...rtant in determining the genetic variation of genotypes among different plant species and its knowledge help the breeders and farmers to select the best variety. Alnus nitida is one of the native and most important plants in District Dir Lower, Khyber Pakhtunkhwa, Pakistan, mostly used for medicinal purposes. In the present study, total 50 genotypes of Alnus nitida were collected from Dir lower and evaluated for morphological traits (leaf, petiole, nut, and ca...

Zhang Dong-jie1, He Xin-miao1,2, Wang Wen-tao1,2 and Liu Di1,2*

...indigenous to China. The genetic background of this breed was previously unclear, limiting the utility of the Min pig. In this study, the whole genomes of ten Min pigs and four Northeast wild boars were sequenced and the analysis yielded 8,988,338 non-redundant SNPs plus 1,231,680 InDels. A phylogenetic tree was constructed and a principal component analysis (PCA) was performed based on previously published SNP data from 66 ...
Hadia Gul1, Abdul Haleem Shah1, Ricardo Harripaul2, Anna Mikhailov2, Ejaz Ullah Khan3, Wasim Shah3, Nisar Ahmad3, John B Vincent2,4 and Muzammil Ahmad Khan3*
... multi-systemic and rare genetic disorder of pigmentation. It occurs due to defects in the melanin synthesis pathway. OCA is characterized by hypopigmentation of hair, dermis, and ocular tissue. The clinical consequences of OCA includes whitish skin, whitish to golden hair and photophobia. Genetic studies have so far reported seven non-syndromic OCA genes, among which Pakistani OCA families mostly segregate TYR and OCA2 gene...

Saydat Saad1, Doaa Abdel-Fattah1, Tarek Khamis2, Aya El-Sobky1* 

...inary physiology, animal genetics, and reproduction. Nano-propolis is beneficial to veterinary medicine in different aspects by varying the size of propolis using various techniques, nano-propolis can be more effective without compromising its qualities. Propolis has numerous benefits, including anti-inflammatory, antioxidant, anticancer, and antifungal properties. Low bioavailability, solubility, absorption, and untargeted control, the advantages of free form...

Hafiz Ghulam Muhu-Din Ahmed1, Aziz Ullah2, Muhammad Asim Bhutta3*, Amna Bibi3, Hafeez-ur-Rehman4 and Umar Farooq4

...t genotypes with diverse genetic makeup were assessed in glasshouse for seedling attributes against limited water conditions using completely randomized design during the season 2019-20 in the Islamia University of Bahawalpur. Based on mean values reasonable variations were noticed in evaluated genotypes for studied attributes. Results from radar analysis, performance designated that the root related attributes were the most sensitive and important attribute f...

Muhammad Tariq Mahmood1*, Muhammad Akhtar2, Kaiser Latif Cheema2, Abdul Ghaffar3, Imtiaz Ali4, Muhammad Jahanzaib Khalid2 and Zeshan Ali5

...that can be deployed for genetic improvement of a species. Screening of available genetic stock for detection of most diverse and high yielding genotypes is a pre requisite for a successful crop breeding program. For this purpose, a research experiment comprising of sixty-eight elite chickpea germplasm genotypes along with two commercial varieties were sown under tri-replicate randomized complete block design during the wint...

Lifang Zhang, Xiuyue Zhang and Bisong Yue*

...ustify;">We analyzed the genetic diversity on Periplaneta americana of 12 populations consisting of 360 individuals with 16 high polymorphic markers. The results showed that the number of alleles per locus ranged from 16 to 32, with an average value 24.5. The observed heterozygosity value (HObs) varied from 0.481 to 0.906 and the mean HObs was 0.698; the expected heterozygosity value (HExp) varied from 0.625 to 0.928 and the mean HExp was 0.840. The polymorphi...

Nafisa Shoaib* and Aisha Majid Ali

...ol for the assessment of genetic damage.
...

Gülseren Kırbas Dogan1,*, Emin Karakurt2, Mushap Kuru3 and Hilmi Nuhoglu2

Maskur Maskur1*, Muhammad Muhsinin1, Lestari, Tapaul Rozi1, Asnawi2 

...ntiation 

...

Shakila Mumtaz1, Khalid Javed1, Muhammad Dawood1*, Muhammad Imran2, Asad Ali1 and Nazia Ramzan1

...ely on the breed’s genetics and ration fed. Different proteins can be found in milk. Beta-caseins are thought to be more important because some serious health-related issues in humans have been reported with the consumption of A1 milk (mutated casein variant). This study was planned to investigate the polymorphism in the beta-casein gene (CSN2) in Sahiwal (40), American Holstein Friesian (40) and the crossbred (Sahiwal × HF) (50). PCR-RFLP and conf...

Shahid Sherzada1,2*, Muhammad Naeem Khan1 and Masroor Ellahi Babar2

...e current study aimed at genetic analysis of by Cirrhinus mrigala using mitochondrial DNA marker cytochrome oxidase I (COI). Genomic DNA isolated from whole blood was used to amplify and sequence a short region of COI gene in mitochondrial DNA. The identification of sequenced samples was done by NCBI (98-99%) and BOLD (99%) databases. The sequence data analysis represented 15 variable polymorphic sites and 4 haplotypes. Mean haplotypes and nucleotide diversity...
Alshimaa A. Hassanien1*, Asmaa Osama Tolba2, Asmaa A. A. Hussein3, Walaa M. Elsherif4
...f 23S rRNA gene and phylogenetic relation between S. aureus isolated from meat products and food handlers. Also, detect the antimicrobial profile and effect of two natural agents of cow milk proteins such as casein and α-lactalbumin on S. aureus isolates of chicken and beef meat and human. Bacteriological culture and PCR were used for S. aureus detection in 150 meat products (50 grilled chickens, 50 grilled beef kofta, and 50 cooked beef meat) as well as...

Zulu Blantina Fangele, Tyasi Thobela Louis, Gunya Busisiwe* 

...en a research gap in the genetic, physiological, and nutritional aspects of indigenous chickens of Africa over the past decade. The use of citric acid has higher economic potential owing to its numerous applications to chickens. This article critically reviews a detailed understanding of the description, advantages and limitations of using citric acid on indigenous chicken. Several studies have been conducted on the nutritional requirements of local chickens, ...

Halidou Maiga Nafissatou1*, Abdou Moussa Mahaman Maâouia2, Moumouni Issa1, Marichatou Hamani1 

...>...
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Asmaa M. Maatouq1, Hoda M. Mekky1, Kh. M. Elbayoumi1,2, Mohamed M. Amer3
...y. Finally, significant genetic changes in our IBDV isolates were observed as comparing to vaccine strains. Thus, continuous monitoring of IBDV outbreaks in vaccinated chickens and evaluation of the used vaccines are required.
 
Keywords | IBDV, Identification, Phylogenic tree, Native chickens, RT- PCR
...

Fan Da1,3, Zheng-Yong Wen1,2*, Xiao-Dong Wang4 and Yu Luo5

... among vertebrates. Phylogenetic analysis suggested the evolutionary process of fish ucp2 was consistent with species evolution, and the pvucp2 shared a close relationship with electric eel ucp2. Quantitative PCRs showed that pvucp2 was extensively expressed in all detected tissues, with the highest expression in liver. Two-week fasting significantly decreased while refeeding dramatically increased the hepatic pvucp2 transcriptions. These findings suggested th...
Mubashir Ali Rather1, Ambreen Hamadani2*, Syed Shanaz2, Nusrat Nabi2, Showkat Ahanger1, H. Hamadani2, Ruksana Shah2
... rich diversity of sheep genetic resources. However, concerning wool traits, the production potential of these breeds was poor. Accordingly, crossbreeding was taken up the breeding policy to improve the genetic worth of these breeds. As a result, a fast-growing and fine wool-producing sheep strain Kashmir Merino was developed. The present article summarizes the performance of this important synthetic sheep strain in the temp...

Hilaire S.S. Worogo*, Thierry O.T.L. Offoumon, Cham D.A. Alabi, Urbain Tchokponhoué, Yaya Idrissou, Alassan S. Assani, Fade Soule, Christophe Iwaka, Ibrahim Alkoiret Traoré 

...ndices for selection and genetic improvement purposes. Body measurements and weights were collected from 108 animals (58 males and 50 females) reared at the Okpara Breeding Farm and ten zoometric indices were then determined. Statistical analyses were carried out using software R. The results obtained showed that sex has a significant effect on the Baron & Crevat index (p<0.01), Compact Index (Cmp.Ind) (p<0.01), dactylo-thoracic index (DTI) (p<0.0...

Hafeez-ur-Rehman1*, Umar Farooq1, Muhammad Asim Bhutta2, Saghir Ahmad2, Muhammad Akram2, Muhammad Rafiq Shahid2, Hammad Hussnain2, Muhammad Shahid2, Muhammad Mehmood Iqbal2, Ali Raza2 and Muhammad Iqbal2

...on variety and also as a genetic resource in various breeding programs with the aim to improve drought tolerance in cotton.

...

Asli Salcioglu

...plotype network and phylogenetic trees show three distinct Spicara haplotypes/haplogroups, corresponding to three different species. Additionally, the number of mutations and high values of sequence divergences were useful in differentiating the three different Spicara.

...

Ejaz Ali and Nageen Hussain*

...r the study of recessive genetic diseases due to a higher rate of consanguinity and Hearing Loss (HL) is one of them that affects more than 466 million people worldwide; will increase to 900 million by 2050. GJB2 mutation is one of the main causes of hearing loss in different populations, including Pakistan, that encodes a gap junction protein involved in the homeostasis of the inner ear by recycling potassium ion. This research aimed to find out mutations in ...

Hua Liu, Wei Hou, Li Peng, Changjun Peng, Yansen Cai and Jing Li*

...le molecular markers for genetic population analysis. SNP markers that are suitable for non-invasive samples are important to wild population investigations in endangered species. Based on the whole genome sequences of Macaca thibetana, we successfully developed 26 SNP loci that were sensitive to non-invasive samples, then based on which, genetic diversity and population structure of three populations of M. thibetana were es...

Roshana Mukhtar1, Shaheen Shahzad1*, Sajid Rashid2, Maryam Rozi2, Madiha Rasheed3, Imran Afzal4 and Pakeeza Arzoo Shaiq5

...) genes. The fundamental genetic cause of CEP has been linked to a sequence variant of UROS on chromosome 10q252-q263. The enzyme catalyzes the fourth step of heme synthesis pathway. The present study focused on the clinical assessment of CEP affected individuals in a Pakistani consanguineous family by Sanger sequencing of UROS gene to identify potential pathogenic sequence variants. CEP patients were identified using successive clinical tests. Blood samples o...

Muhammad Zahid Iqbal1, Aneela Zameer Durrani1, Jawaria Ali Khan1, Nisar Ahmad2, Muhammad Usman1,*, Abdul Jabbar1, Saba Usman3, Ahsan Anjum3, Muhammad Husnain1, Nadeem Raza1 and Anwar-ul-Haq4

... determine phylogeny and genetic variability of various isolates identified during the study, and report hematology of the affected bovines. The incidence of coxiellosis was estimated as 32.12% and 12.5% in cows and buffaloes, respectively. The association of selected clinical biomarkers was also ascertained and all of these were found significantly (p<0.05) associated with the incidence of coxiellosis. The results of animal-related risk factors indicated t...

Riffat Sultana1*, Samiullah Soomro1 and Chuan Ma2

...es indicating negligible genetic divergence and very close resemblance with 99.85% identity between C. (Chrotogonus) h. homalodemus (SWF and LWF) against RID-MMV2XTNY114 and 99.85% identity between C. (Chrotogonus) t. robertsi (SWF and LWF) against RID-MMVW0MTF114. Finally, we discuss the possible taxonomic implication of our DNA sequencing results and point out future research directions.

...

Junyan Bai*, Jingyun Li, Yu Chen, Zhihao Dong, Youbing Yang and Ying Lei

...loyed to investigate the genetic polymorphism of sheep GH gene and to analyze the correlation of genetic polymorphic sites with growth traits. The results showed that AA, AB and BB genotypes were detected in exon II of GH gene in the six sheep populations. The highest frequencies of AB genotype detected in Mongolia sheep, Small-tailed han sheep, Tong sheep, Lanzhou large-tailed sheep and Henan large-tailed Han sheep were 0.3...

Dwi Budiono1,5, Ratih Rinendyaputri2, Ariyani Noviantari2, Mokhamad Fahrudin3, Ni Luh Putu Ika Mayasari4, Vista Budiariati1,6, Diah Nugrahani Pristihadi1,3, Noer Muhamad Dliyaul Haq1, Arief Boediono3* 

...lony 

...

Aml M. Ragab1*, Maha R. Basyoni1, Enas A.I. Khoris2, Nadia A. Abd Elghany3 

...the groEL gene as a phylogenetic marker, and identified (hbl ,(nhe), (cytK), and (ces) enterotoxigenic genes. B. cereus isolates were analyzed for antibiotic susceptibility. A lab trial was conducted for two weeks using 60 Tilapia fish were divided into three equal groups, (1): kept as control negative, (2): infected intraperitoneally with (0.1ml) 8×107 (CFU/ ml/ fish) B. cereus on 1st day, (3) infected I/P intraperitoneally with (0.1ml) 8×107 (CFU...

Md. Taimur Islam1*, Mirza Mienur Meher2, Anas Bin Harun3 and Md. Golam Haider1

 

... and thereby changed its genetic clades. The prevalence of ND is highest in October and in 0-3 months aged chickens. AMPV generally infects the upper respiratory tract of poultry, which has been isolated for the first time in Bangladesh in 2016. Alternatively, adult chickens are more susceptible to IB and ILT. The prevalence of mycoplasmosis and aspergillosis are higher during the winter season and are reported to occur due to poor management systems. Overall,...

Weidong Huang1,2,3, Xinyue Liang1,2,3, Xiufeng Xie4, Xingmin Wang2, 3 and Xiaosheng Chen1,2,3*

...d beetles. However, phylogenetic relationships and genetic diversity of discrete color patterns in coccinellids is still poorly known. Here, we present a comprehensive genetic diversity analyses and phylogenetic relationships within seventeen different phenotypes of elytral color patterns in Menochilus sexmaculatus based on two mitochondrial genes, cytoc...

Maria Qibtia1, Sehrish Faryal1, Muhammad Wasim1, Farzana Chowdhary1, Muhammad Tayyab1, Ahmed Mansovuri2, Zeeshan Ahmed2, Muhammad Hamid3 and Ali Raza Awan1,*

...se persistence (LP) is a genetically determined phenotypic trait, generally related to pastoralism and milk consumption. However, Lactose non-persistence (LNP) results from the progressive decline of Lactase-phlorizin hydrolase (LPH) activity in enterocytes. The Single Nucleotide Polymorphism (SNPs) associated with occurrence of LP/LNP are different for European, Asian, African-American, Northern African and Arab populations. In this study, we studied, the ass...

Zaniar A. Abas1, Mohammed Omer Baba Sheikh2,5*, Hardi N. Aziz3, Omed I. Abid4 

...ation study based on the genetic variation on the VP2 gene has shown that (CPV-2/Sul) strain belongs to new CPV-2b and CPV-2c, however, the (CPV-2/Erbil & CPV-2/krk) strains belong to new CPV-2a. A Phylogenetic tree constructed basis on the VP2 gene revealed that the field virus sequences from the present study were divided into two main phylogenetic groups (cluster A and cluster B), 1...

Nour H. Abdel-Hamid1*, Walid Elmonir2, Eman I. M. Beleta1, Rania I. Ismail1, Momtaz Shahein1, Mahmoud E. R. Hamdy1 

...16 and 13 genotypes with genetic similarity percentages of 62% and 54% with a diversity index of 0.82 and 0.88. Both ERIC-PCR and RAPD-PCR dendrograms clustered B. abortus isolates into two clusters and three genotypes with discriminatory of 0.8 and 0.72. Both ERIC and RAPD PCRs revealed 25 singleton genotypes (G) out of 35 genotypes. DNA-based typing techniques showed substantial diversity among the Brucella isolates. The genetic

Zilu Zhang1, Ning Wang2, Ce Hou1, Laiba Shafique1, Saif ur Rehman1, Zhuyue Wu2, Zhiqiang Wang1, Mingsong Wei3* and Kuiqing Cui1*

...of Guanzhong dairy goat, genetic diversity in PIS region of the goat were identified and the key gene polymorphism sites still waiting to be determined.

...

Rabia Goher, Inamullah and Mohammad Akmal*

...n: justify;">Despite the genetic potential, the yield performance of a genotype is expressed in the prevailing environments. A high yielding genotype may not necessarily reflect its potential performance when subjected to a different Agro-Environment (AE). This study, therefore, aimed to assess the foremost developmental stages of the selected genotypes of wheat in three AE i.e. AEP (Peshawar), AEK (Kashmir) and AEC (Chitral). An experiment was conducted in a ...

Ghassan Zahid1, 2*, Sara Iftikhar3, Muhammad Umer Farooq4 and Shakeel Ahmed Soomro5

...lications in the area of genetic diversity, varietal identification studies, hybrid detection, disease diagnostics and sex differentiation by administering the powerful diagnostic polymorphism detection tools at specific loci and the genome level. This review focuses on exploring the application, recent advancements and future implications of DNA-based molecular markers with the context of their role in the genetic improveme...
Mudassar Jehan1, Masroor Ahmed Bajwa2, Mohammad Masood Tariq2, Asim Faraz3*, Abdul Samaad2, Jameel Ahmad1 and Yousaf Hassan Barozai2
...esent study to highlight genetic polymorphism. The PCR was utilized for amplification of individual DNA samples. All of the 15 SSR markers were amplified. After gel documentation, a total number of 97 alleles were recognized having 1 to 5 polymorphic forms (OARFCB193, OARJMP29, MAF33) to 4 (OARHH47, DYMS1, SRCRSP5). For total loci, number of alleles averaged 2.1162±0.3769. Shannon’s Information index (I) 0.6184±0.2447 and the effective numb...
Zayed Mohammad Ali I.1*, Mona Mohamdy1, M.F. Shehata1, Nasser Ghanem2
...ify;">Feeding regime and genetic potentiality for muscle development and growth are major factors affecting sheep meat production. Therefore, the aim of this study was to evaluate the potential of Abo-Deleek lambs in meat production under different management systems. Sixteen Abo-Deleek lambs aged six months were used in the present study. Experimental lambs were divided according to body weight into two similar groups and then randomly allocated to two differ...

Mohamed El-Henfnawy*, Essam A. El-Gendy, Ahamed M. El-Kaiaty, Mostafa Helal 

...rs to the interaction of genetic architecture with male or female performance. The current study aimed to evaluate the genotype-by-sex (G×S) interaction on the growth performance of a selected chicken line and its genetic control line. The results revealed significant G × S interactions for body weights at 4 and 10 weeks of age. The line effects on body weight traits were significant and consistent except for 2-w...

Chandni Wajid1, Abdul Hameed Baloch1, Ahmed Nawaz Khosa1*, Hubdar Ali Kaleri2, Nasrullah Bangulzai1, Sarfraz Ali Fazlani1, Haleema Sadia3, Saeeda Kalsoom4, Muhammad Bilawal Arain2, Wassem Ali Vistro2 

...oduction in mammals. The genetic variations in PROP1 gene in the sheep breeds of Pakistan are unknown. This study was conducted to compare wool color and fleece weight and to analyze the PROP1 gene of Balochi, Bibrik, Harnai and Rakhshani sheep breeds of Balochistan. The wool was sheared, its color and fleece weight were observed. The result reveled that wool color and fleece weight were black and white with 1.91 kg, full white, white with black muzzles and li...
Mohamed Lebdah1, Sahar Abd El Rahman2*, Ahmed Attia3, Reham Karam2, Naglaa Fathy Saeed Awad1, Mohamed Ibrahim El Bagoury1

Muneeza Zafar1,2,3, Fazli Rabbi Awan2,*, Munazza Raza Mirza3,*, Sumaira Nishat2,4, Sajid Ali Rajput5 and Imran Riaz Malik1,*

...be prioritize for future genetic association studies in cohort of cardiovascular patients. While their structural impact on APOB may suggest these predicted nsSNPs possibly be a better drug target and contribute to the treatment and better understanding of human cardiovascular disease.

...

Naseem Sharif1,2*, Imran Muhammad Siqqique2, Muhammad Kashif Raza3, Urwa Irshad1, Muhammad Ikhlaq Khan4, Ammara Noreen4 , Muhammad Ahsan Qureshi1, Mohsin Abbas5, Muhammad Maaz Aziz5, Sitwat Riaz5, Komal Aslam5 and Naseem Akhtar6

...ly favoured to save rich genetic resources of this unique jujube specie.

...

Mohamed. E. Taha1, Mohamed S. Ahmed2, Ahmed I. Ahmed2, Amany Adel3, Salama Rehab4,5*, Nabila Osman2** 

...; were mutated. The phylogenetic analysis clearly highlights the aggregation of the sequenced AIV H9 isolates with the Egyptian H9N2 viruses G1 lineage in the group B and the Israeli strains. Although the strains are low pathogenic, the presence of mammalian binding amino acid markers indicated their public health concern. Overall conclusion indicated that AIV (H9N2) still circulates among poultry farms in the Southern Egypt. Furthermore, isolated strains were...

Nishant Shah1*, Rakesh Kumar Yadav1, Anil Gautam1*, Shambhu Shah1 and Mohan Kumar Gupta2

... do not perform to their genetic potential in a stressful and diseased condition as concerned with poor bedding materials, high ammonia production from litter, and so on. The maximum ammonia production from litter depends on factors like moisture (%), pH, total nitrogen (%) of litter, and temperature of broiler houses. Carbonized Rice Husk (CRH) is a good poultry bedding material that consists of carbon and SiO2 which interestingly enhance ammonia adsorption. ...

Sri Melia1*, Indri Juliyarsi1, Yulianti Fitri Kurnia1, Evy Rossi2, Hurriya Alzahra3

...umonia. Base on the phylogenetic analysis, the bacteria isolated were closely related to Limosilactobacillus fermentum, a probiotic bacteria candidate.
 
Keywords | Lactic acid bacteria, Limosilactobacillus fermentum, Antimicrobial, Probiotic
...
Amreen Zahra1*, Mushtaq A. Saleem2, Hasnain Javed3, Muhammad Azmat Ullah Khan4 and Abdul Rauf Shakoori4
...ar insight unveiling the genetic characteristics of the prevailing strains is the need of the hour for successful disease control interventions in endemic countries like Pakistan. Here we present, a detailed molecular and computational analysis of the major genetic constituents, Gag-Pol region of HIV-1 for the very first time in Punjab-Pakistan including three regions of outbreak i.e. Kot Imrana Sargodha, Faisalabad and Gujr...
Dalia M. Aboelhassan1*, Inas S. Ghaly1, Noha E. Ibrahim2, Nermeen M. Shaffie3, Mariam G. Eshak1, Aboelfetoh M. Abdallah4, Ibrahim M. Farag1
...athology in rats. In the genetic study, the assaying of expressions of IkBα and COX-1 genes in liver tissues, as well as the expressions of mdr1a and COX-1 genes in kidney tissues are evaluated. In addition, the histological architectures of these organs are examined. Bisphenol A (BPA) treatment causes over-expressions of the above mentioned genes, and induces massive damage to the histological architectures of liver and kidney tissues. In contrast, FCE ...
Aiman Al-Mufarji1, Abd El-Nasser Ahmed Mohammed1*, Haitham Al-Masruri2, Rashid Al-Zeidi3
Zeinab R. Aboezz1*, Ayman S. El-Habbaa1, Rania S. El-Mohamady2, Samia A. Elnagar2, Ehab M. El-Nahas1
...Pestivirus, Cattle, Phylogenetic analysis, 5`UTR
...
Basel A. Abokhadra, Samah M. Mosad, Sahar Abd El Rahman*
...e the same antigenic and genetic relatedness. BoHV-1 is one of the viruses that incriminated in respiratory disease; affects the genital tract and causes significant economic losses to domestic cattle in Egypt. Although BoHV-5 mainly causes encephalitis in calves, the virus is excreted in nasal discharge under stress factors, so herpesvirus latency reactivation cycle is the hallmark of the disease. In the present study, molecular screening of 60 nasal swabs, c...

A. El-Shemy1*, Hoda M. Mekky2, M. A. Bosila2, A. M. Allam1, Kh. M. Elbayoumi2, M. M. Amer3 

...Egypt. However, the phylogenetic relationship using the partial 3D protein revealed that the isolated Egyptian strains in this study were distant from the vaccine strain used in Egypt. In conclusion, three isolates of DHAV-1 were characterized from the examined duck flocks. Phylogenetic analysis of these isolates revealed the occurrence of differentiation between our field DHAV-1 isolates and the vaccine strain. Therefore, a...

Ngoc Tan Nguyen1*, Minh Thanh Tram1, Thi Thu Pham1, Tan Loi Le1, Thi Khanh Ly Nguyen1, Tuan Thanh Hoang2, Cong Thieu Pham3, Nguyen Khang Duong4 

... haplotype diversity and genetic distance to construct a phylogram. Results showed that the fragment of 687 bp was successfully amplified. Examination of 49 sequence variants in 599 bp of the control region revealed the nucleotide composition was Adenine (A) = 32.55%, Thymine (T) = 28.33%, Guanine (G) = 14.67%, Cytosine (C) = 24.87% and G+C content was 39.12%. Out of a total of 50 nucleotide polymorphic sites, 48 were transition and 2 were transversion, with 2...

Limei Yuan1, Kong Yang1* and Dechun Jiang2*

...ationships, and the phylogenetic status of many species are still unclear, which hampered the taxonomy and protection of these species. In this study, we investigated the molecular phylogenetic status of R. laoshan and Z. yinggelingensis using mitochondrial DNA (12S rRNA, tRNAVal, 16S rRNA and Cyt b) fragments and nuclear DNA (RAG-1, RHOD, TYR) fragments. Our results revealed that Rhacophorus laoshan is closely related to R....

Madiha Khalil1, Amjad Farooq1, Asim Faraz2*, Abdul Waheed2, Nasir Ali Tauqir3, Riaz Hussain Mirza2 and Hafiz Muhammad Ishaq2

...stan and to evaluate non-genetic factors affecting the lactation traits. For this purpose, data on lactation performance of Holstein Friesian cows were collected from government livestock farms located at Quetta. Data obtained on calving from 608 cows during last sixteen years (2000-2016) were analyzed. After editing the data for consistency and errors it was subjected to statistical analysis using fixed effect models. Independent variables were year of birth,...

Abdul Ghaffar*, Niaz Hussain, Muhammad Nadeem, Khalid Hussain, Muhammad Aslam, Mudassar Khaliq, Muhammad Irshad, Zubeda Parveen and Muhammad Younas

...ble selection method for genetic improvement in chickpea. The current investigation was conducted at the research area of the Arid Zone Research Institute, Bhakkar, Punjab, Pakistan. Twelve chickpea genotypes were sown in RCBD following three replicates. Data regarding primary & secondary per plant, days for maturity, plant height as well as potential yield were recorded. The measured data were analyzed to principal component analysis (PCA), correlation, a...

Umair Faheem1*, Qurban Ali2, Mussurat Hussain1, Abrar Ahmad3, Tamsila Nazir2, Ghayour Ahmad3, Idrees Ahmad3, Madiha Mobeen4, Hammad Hussnain3 and Nadia Hussain Ahmad3

...sts. Cotton plants shows genetic resistance or tolerance against these insect pests. In the current experiment six varieties of cotton i.e. CIM-496, CIM-534, NIAB-111, MNH-786 and Bt-121 were sown in the field under sprayed and un-sprayed condition to check the genetic resistance or tolerance against these insect pests and to also check the population of the parasitoids. It was observed that MNH-786 and Bt-121 were the most ...

Syed Wajahat Husain Jaafry1* and Amber Fatima2

...ors at various points of genetic affiliation. Contrasting results and lack of molecular evidence in species recognition studies have made this topic more curious and complex. Some plant species compete more vibrantly for below-ground resources when an encounter with neighboring unrelated conspecifics as compared to related conspecifics (kin recognition). Some species increase competition when interacting with related and unrelated conspecifics (niche partition...

Kusuma Adhianto*, Chairul Rahman Arif, Dian Kurniawati, Akhmad Dakhlan 

...lect livestock with good genetic quality. This study aims to determine the genetic potential of male and female Saburai goats that have good genetic quality at weaning based on their estimated breeding value (EBV) and to determine which individuals are eligible to be retained in the population. Materials and Methods: This research was conducted in March—May 2021 at Gisting Atas Villa...

Ahmed Abd El-Salam1*, Yasser Hamouda1, Salah El-Safty2, Ahmed El-Attar2

Ali Basim Abd Al-Hadi*, Tahreer Mohammed Al-Thuwaini, Mohammed Baqur Sahib Al-Shuhaib
...tion rate, hormones, and genetics. Genetics and endocrine signal transpositions between the pituitary and the ovary play a crucial role in the reproductive traits of sheep. Genetic polymorphism of candidate genes is associated with economic traits. Follistatin (FST) gene is one of the most notable potential candidate genes linked to economic traits. This study aimed to assess the

Muhammad Jamil1*, Kamran Javed1 and Imran Akhtar2

...rpose was to explore the genetic diversity in the strains under study. Principal component analysis, along with agglomerative hierarchical clustering tools were employed. Twenty-four recently bred upland cotton strains bearing diversified origin were configured in triplicate following Randomized Complete Block Design (RCBD). The data for novel attributes were recorded. Analysis of variance results indicated that a significant level of variation was existing am...

Sarhad Journal of Agriculture

June

Vol. 38, Iss. 2, Pages 388-758

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