Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
Life Sciences International Journal Issue 7 Volume 1
...d 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serot...
Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad
British Journal of Virology
... 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serot...
Souvik Ghosh, Nobumichi Kobayashi
British Journal of Virology
... Although studies on the genetic diversity of the antigenically important RVA VP7- and VP4- protein encoding genes are important for vaccine development or judging the efficacy of existing RVA vaccines, they do not always provide conclusive information on the overall and complex genetic makeup of RVAs, as the remaining 9 RVA gene segments are also susceptible to the forces governing RVA genetic
Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
British Journal of Virology
...d 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serot...
El-Sayed M. Abdelwhab, Jutta Veits and Thomas C. Mettenleiter
Avian Influenza H5N1 in Egypt: What we Know and What we have to Know?
...ible to oseltamivir, but genetic markers indicating resistance to amantadine have increased dramatically. In this mini-review, we discuss recent findings on the H5N1 viruses endemic in poultry and humans in Egypt, and suggest possible options to control the infection....
Yanina Panzera1*, Nicolas Sarute1, Lucia Carrau1, Jaime Aldaz2, Ruben Perez1
...detailed analysis of the genetic diversity, evolution, and epidemiology of the virus in several countries. Our study allowed us to establish the presence of four genetic lineages in South America, being the continent with more lineages described to date, underscoring the high genetic diversity of the circulating strains....
Hanqin Shen1,3, Boliang Wu1,2, Guangwei Li1, Feng Chen1, Qingbin Luo1,3, Yimin Chen1, Qingmei Xie1,2,3*
...essure of vaccination by genetic evolution and antigen variation. Moreover, the ratio of L226 in the haemagglutin (HA) receptor binding site has increased, which indicates that the H9N2 virus has the increasing potential to infection and cause human epidemics. The threat to the poultry industry and public health should not be ignored. In this review, we summarised the findings of recent studies on H9N2 AIVs epidemics and strategies for their prevention in poul...
Xingdong Yang and Lijuan Yuan
...s and humans in terms of genetics, physiology, intestinal anatomy, and immune system, pig is particularly a suitable animal species for the modeling of human enteric pathogen infections, immune responses and for vaccine evaluations. These are the basis for the successful establishment of the infection and disease models for human rotavirus, human norovirus and human enterovirus 71 using neonatal gnotobiotic pigs. The differences between germ-free and normal an...
Weili Kong1, Guangpeng Ma2 and Jinhua Liu1*
...NS1 protein in different genetic backgrounds of influenza experimentations. Conclusively, it can be stated that CTT confers a positive role during the infection of influenza A viruses. However, this regulation is contributed by multiple factors and has variable impact on the pathobiology of influenza A viruses....
Mohammed Ali Hussain, Zakiya Ahmed Hassan
Gerald Misinzo1,*, Tebogo Kgotlele1, Epaphras A. Muse1, Jan Van Doorsselaere2, Mikael Berg3, and Muhammad Munir4
...eotide sequence and phylogenetic analysis clus- tered the PPRV from Tandahimba into lineages II and IV. The results give evidence of at least two separate introductions of PPRV into Southern Tanzania, underlining the transboundary nature of the disease, particularly in regions with uncontrolled livestock movements....
Nayle Maria Oliveira da Silva1*, Ricardo do Carmo Zanella2
Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*
...further pathological and genetic researches in Taiwan. Here, bioassay, multiplex RT-PCR and multiplex real-time RT-PCR were used to detect the current status of the two viroids showing HSVd was more prevalent than CEVd and simultaneous infections were common in field. Uneven distributions of both viroids also occurred in different tissues of citrus. Two multiplex molecular detection methods provide the understanding of the genetic
Galina N. Leonova1*, Ilya G. Kondratov2, Sergei I. Belikov2
...fying the biological and genetic characteristics of three Louping ill virus (LIV) strains in the south of the Russian Far East isolated from Ixodes persulcatus ticks and one strain was isolated from the blood of a person after a tick bite. Phylogenetic analysis showed that these strains are related to LI/A and LI/K strains isolated in England and Scotland.
...
Shahid Iqbal Awan1*, Syed Dilnawaz Ahmad2, Muhammad Amjad Ali3, Muhammad Shahzad Ahmed1, Aurangzeb Rao1
...er analysis, and greater genetic distance was detected among the members of cluster 6 and cluster 8 and cluster 7 and 8. Members of these two clusters can be utilised in transgressive breeding....
Temitope Oluwasegun Cephas Faleye1, Johnson Adekunle Adeniji1, 2 *
...ed and subjected to phylogenetic analysis. Both sites yielded two isolates each on MCF-7 cell line while on RD cell line one site yielded one and the other seven isolates. None of the twelve isolates replicated on L20B cell line. All were positive for both the panenterovirus 5l-UTR and partial VP1 PCR reactions while only the four isolates recovered on MCF-7 where positive for the EV-C-3Dpol/3’-UTR-PCR assay. The eleven isolates with exploitable VP1 sequ...
Ummara Waheed Khan, Raza Ahmed, Irum Shahzadi and Mohmmad Maroof Shah
Email | mmshah@ciit.net.pk
...e discovery of molecular genetics and biotechnological techniques, such as genome mapping, tissue culture, genetic transformation and in vitro regeneration offered new dimensions of research to improve important crop species including wheat. The tissue culture by itself has increased the crop improvement potential to develop new cultivars with desirable traits through somaclonal and gametoclonal selection...
Tauseef Taj Kiani1*, Mozammil Hussain1 and Hidayat ur Rahman2
...actical contributions of genetics to the agricultural world and has its most signi cant expression in maize than other cereal crops. Exploitation of this phenom- enon of superior performance of F1 progeny over their parents not only trans gured maize breeding schemes but also constituted the foundation of maize seed industry. Evaluation of per se performance of parental lines and estimation of heterosis and inbreeding depression are prerequisites to start hybr...
Mohammed A. Rohaim1*, Rania F. El Naggar2, Ahmed M. Helal3, Hussein Ahmed Hussein1 and Neil LeBlanc4
...the first pathotypic and genetic characterizations of the pigeon paramyxovirus type I (PPMV-1) from pigeon in Egypt during 2014. Pathotyping, based on the mean death time (86 hours) in eggs and the intracerebral pathogenicity index (1.2), and the amino acid motif, (112KRQKR116), at the cleavage site of the fusion (F) protein indicated the emergence of PPMV-1 in Egypt. Phylogenetic analysis of the F and haemagglutinin-neurami...
Mohammed M. Islam1, 3 *, Damian F. J. Purcell2 and George Kannourakis1,3
...ntify viruses of diverse genetic backgrounds. ...
Tahir Khan, Raziuddin, Fakharuddin* and Muazam Jamal Razi
...as conducted to estimate genetic variability, heritability and genetic advance for important attributes in Brassica napus using 10 parental lines and their 21 F4 populations. Significant differences were observed among genotypes, parents, F4 populations and parents versus F4 populations for days to 50% flowering, days to maturity, plant height, primary branches plant-1 and m...
Ashley C. Banyard1* and Satya Parida2
...with both viral and host genetics being thought to influence the outcome of disease. Following the successful global eradication of rinderpest virus, peste des petits ruminants virus has also been targeted for global eradication by the OIE. Whilst the tools are available to attempt eradication there are a number of factors that will influence the strategy taken to progress with the aim of eradication. Here we describe current areas where knowledge ...
Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*
...es and peptides, reverse genetics, anti idiotypic antibody based vaccines and helper cell dependent diagnostics represent the present vaccine/diagnostic development strategies for the effective control and eradication of PPR. This review comprehend the current scenario of recombinant technology based vaccines and diagnostics, virus like particles (VLPs), reverse genetics approach etc., in the development of diagnostics and v...
Mohammad Mushfiqur Rahman1, Rokshana Parvin1, Ataur Rahman Bhuiyan1, Mohammad Giasuddin2, Shah Md. Ziqrul Haq Chowdhury3, Mohammad Rafiqul Islam1, Emdadul Haque Chowdhury1*
...were sequenced. The phylogenetic analysis revealed that PPRVs circulating in Bangladesh belonged to Lineage IV and they formed a separate sub-cluster along with recent isolates from Nepal, Bhutan and China. On genetic analyses, it appeared that the N gene of PPRV is less conserved as compared to the F gene. Two unique amino acid substitutions (K423Q and E426G) were observed in Bangladeshi and Chinese isolates in the partial ...
Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
Muhammad Jurial Baloch1*, Ghulam Murtaza Channa2, Wajid Ali Jatoi2, Abdul Wahid Baloch1, Imdad Hussain Rind1, Muhammad Ahmed Arain1 and Ayaz Ali Keerio1
Christopher U Orji1*, Ignatius O Onyeocha2, Steven S Shaida3, Peter M Dede4, Bitrus Yakubu5, Elijah E Ella6 and Pam D Luka5
...n rely upon knowledge of genetic diversity between species’ population. Two species were studied: Glossina palpalis palpalis and Glossina morsitans submorsitans using two mitochondrial DNA fragment primers- Cytochrome oxidase subunit II (COII) and Cytochrome b (CytB). Twelve samples of laboratory-reared population of each species were used for the study. Sequencing data were used to calculate haplotype, haplotype diversity and nucleotide di...
Zuhao Huang1, Feiyun Tu2 and Dianhua Ke1*
...aphical distribution and genetic data suggest the elevation of M. v. americanus to a full species Rufous-crowned Bee-eater M. americanus, supporting the latest taxonomic arrangement by IUCN....
Iqra Mahmood1, Asif Nadeem1*, Masroor Ellahi Babar2, Muhammad Muddassir Ali1, Maryam Javed1, Aisha Siddiqa1, Tanveer Hussain2 and Muhammad Tariq Pervez2
...nd to identify potential genetic markers for resistance to mastitis. This article presents a systematic integration of complex biological interactions of 226 mammary gland genes to uncover underlying regulatory networks. Data were collected from various databases after having an exhaustive literature study. Network, functional and pathway analysis of the mammary gland genes were performed to find mastitis resistant genes, which helped us to identify prime gene...
Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3*
...don usage analyzed. Phylogenetic Bayesian and ML analyses were conducted, using a concatenated set of 12 protein-coding genes, and adding 16 other species of gobies (Gobiidae). The mitogenome sequences of O. lacepedii,
Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2
...s study were to identify genetic polymorphisms of the huntingtin (HTT) gene and to further investigate its association with production performances in two Anhui local chicken breeds. A total of 800 chickens (400 Huangshan black chickens and 400 Huainan partridge chickens) of similar age and weight were evaluated in the present study. We extracted DNA from blood samples and analyzed polymorphisms of HTT gene as wells as possible association with egg production ...
Ali Raza Awan*, Sehrish Firyal, Muhammad Tayyab, Lala Rukh, M. Zia ul Haq, Shagufta Saeed and Muhammad Wasim
...ore. This study aimed to genetically identify and classify the indigenous wild Rose-ringed Parakeet of Pakistan using Cytochrome b (Cytb) gene polymorphism. Mitochondrial DNA of 24 unrelated Pakistani wild Ring-rose Parakeets was isolated and utilized for amplification and DNA sequencing of Cytb gene. The Phylogenetic analysis of the Cytb gene indicated that the Pakistani wild Rose ringed Parakeet was mono-phyletically clade...
Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3
...arkers in genotyping and genetic identification is of essential significance for preserving genetic diversity. The aim of this study was to reveal the genotype profile of indigenous types for TGC breed. In order to characterize TGC population, two polymorphic loci in cattle Calpain gene (CAPN1) and one polymorphic locus in Calpastatin gene CAST were studied. In order to sort the allele variants (G/C), PCR products, the ones ...
Imed Ben Salem*, Aymen Ben Ibrahim, M’barek Chetoui and Said Nouira
... study deals with morphs-genetic analysis of eight species viz. Lemniscomys barbarus, L. bellieri, L. griselda, L. limulus, L. macculus, L. rosalia, L. striatus and L. zebra; which have practically similar external morphologies although they spread in completely different geographical areas. The approach adopted was to identify landmarks on the skulls of all specimens using the tpsDig software, and then analyzing them through the program MorphoJ. Our results s...
Atta ur Rehman1, Sher Ali2 and Sajid Malik2*
... In order to observe the genetic differentiation in lower Khyber Pakhtunkhwa (KPK) region, ABO and Rh blood groups record of nine Pashtun populations, i.e., Bannu, Dera Ismail Khan, Hangu, Karak, Kohat, Kurram Agency, Lakki Marwat, South Waziristan Agency, and Tank, was collected. Absolute gene diversity, gene differentiation and genetic diversity were estimated to be 0.01, 0.019 and 0.515, respectively. There were the highe...
John G. Bruno
...erotypes if coupled with genetic identification techniques such as PCR or gene probe hybridization....
Fukuan Du1,2, Yan Li2, Zhixin Wen1, Ruguo Chen1 and Pao Xu2*
...very urgent to carry out genetic breeding to improve yield. In this study, we developed SSR markers by transcriptome, which could be used to genetic research. In this study, we performed transcriptome sequencing of six libraries from hepatopancreas samples in E. sinensis. After removing low-quality reads, short reads and reads belonging to mitochondria, a total of 278,260,960 clean reads corresponding to mRNAs were obtained,...
Bahattin Cak1, Orhan Yilmaz1, Siddik Keskin2, Tahir Bayril3 and Mohammad Masood Tariq4*
...considered in subsequent genetic evaluations. Furthermore, producing heavier carcasses (13-17 kg) in < 150 days may increase productivity and efficiency of the goat farming system....
Muhammad Saeed* and Iftikhar Hussain Khalil
...icated that non-additive genetic expression were pre-dominant for most of traits studied. Line BRF3 and testers ZM04 were good general combiners for most of traits under consideration, while F1 hybrids B4N11 × PS08, B4N11 × JZ, BRF1 × ZM04, BRF1 × KT10 were best specific combiners observed for several important traits including grain yield plant-1 which can be subsequently utilized in future wheat breeding to develop high yielding new w...
Sheraz Ahmad Khan and Ghulam Hassan
...imation of heritability, genetic advance and association of yield and yield related traits in wheat. For all the studied traits, mean squares showed the presence of significant variation (p≤0.01) among the genotypes. Heritability estimates were observed high (h2˃0.60) for all the traits. The highest heritability (0.93) was noticed in plant height followed by flag leaf area (0.89). The values of genetic advance were r...
Ibni Amin Khalil* and Raziuddin
... as well as non-additive genetic mechanisms were found important due to significant effects for both components i.e. General Combining Ability (GCA) and Specific Combining Ability (SCA) effects. Maximum GCA was shown by L-7 and T-1; whereas high SCA was noted for L-6 and L-7 with good ability to combine with T-3 and T-1 respectively, and could be utilized as genetic material in future breeding programs. Overall the biplot ap...
Waheed Anwar*, Muhammad S. Haider, Ahmad A. Shahid, Hamid Mushtaq, Usman Hameed, Muhammad Zia Ur Rehman and Muhammad Javed Iqbal
...rphologically as well as genetically by amplifying internal transcribed spacer region (ITS). Variability among Fusarium species based on ITS showed that it is not enough to score diversity within species and isolates. Additionally pathogenicity of isolated Fusarium species was evaluated against nymph and adult of Bemisia tabaci. Under controlled conditions different species of Fusarium restrained the growth of B. tabaci
Lingtong Ye1, Chao Cao1,2, Bin Tang1,2, Tuo Yao1, Ruixuan Wang1 and Jiangyong Wang1*
...). The intraspecific genetic distances of polydorids examined in the present study ranged from 0.33% to 1.67%, whereas the interspecific distances ranged from 18.88% to 24.79%. Such large barcoding gaps between intra- and inter-specific distances indicate that the COI is a suitable gene marker for molecular identification of polydorid species. Our results demonstrate that not only did all COI sequences from the larvae show greater than 99% sequence identit...
Guang-Xin E1, Yong-Ju Zhao1, Zhong-Yuan Zhang1, Yan-Guo Han1, Wei-Wei Ni1, Ming-Xing Chu2, Yue-Hui Ma2, Jian-Ning He3, Huai-Zhi Jiang4, Jia-Hua Zhang1, Zhong-Quan Zhao1, Yan Zeng1, Ri-Su Na1, Gao-Fu Wang5, Hang-Xing Ren5 and Yong-Fu Huang1*
Pam D Luka, Frank N Mwiine, Bitrus Yakubu, Joseph Erume, Ricardo Pérez-Sánchez, Hermann Unger and David Shamaki
...fluence the emergence of genetic variation and long-term persistence of the virus in an area. Since the first report of ASF in Nigeria, periodic outbreaks have continued to occur among domestic pigs with a few incidences in wild pigs. However, to investigate the risk of a tick-domestic pig transmission cycle in Nigeria, 3,288 serum samples were collected from domestic pigs from 10 states and analysed for anti-tick antibodies using the recombinant TSGP1 indirec...
Ali Zurwan1,*, Muhammad Moaeen-ud-Din2, Ghulam Bilal2, Zia-ur-Rehman1,2 and Muhammad Sajjad Khan1
...67 indicating very lower genetic control. This is the first study to show genetic of persistency of lactation. Although results may be validated using more data and more accurately recorded pedigree for several generations however, this is really useful firsthand information.
...
Nagwan F. Zahran, Ali F. Hamza and Rehab M. Sayed*
Waqar Islam*1, 2, Madiha Zaynab3, Muhammad Qasim2 and Zujian Wu1,2
... involving cis and trans genetics. The review adhere recent research findings regarding disease resistance against viral diseases and concludes via shedding light upon the future prospects in this exciting field of research.
...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...otyping of SCARB1 genetic polymorphisms was done by PCR-RFLP combined with gel electrophoresis. The genotype distribution in the population was found to be consistent with Hardy Weinberg equilibrium. Through logistic regression analysis, it was observed that, in Tamil population the CC and CT genotype carriers have higher odds of developing myocardial infarction (OR – 1.64 and 2.04 respectively) and for developing hypercholesterolemia (OR &n...
Tianzhu Chao, Huiqiang Cai, Yuxun Zhou, Kai Li* and Junhua Xiao
...gated the molecular phylogenetics of Chinese house mice based on 466 DNA sequences from mitochondrial D-loop fragments. Our analyses revealed that Mus musculus musculus and M. m. castaneus are older colonized subspecies with broad distributions in East China, while M. m. domestics is a newly arrived subspecies currently restricted to Shanghai, indicating the elevated risk in this cosmopolitan cities. Phylogenetic
Jun Gao1,2, Liang-Liang Yue3, Xianhuan Jiang1, Liju Ni4, Muhammad Aqeel Ashraf5, Yuxun Zhou,1 Kai Li1,* and Junhua Xiao1,*
... habitat has resulted in genetically distinct populations. The trees, inferred from maximum likelihood and Bayesian phylogenetic analysis, highly supported all the M. fortis individuals clustering into one monophyletic lineage. Three main clades are recovered within M. fortis: (1) North group; (2) South group; and (3) GX group. The North Group distributed on the north side of Qinling Mountains-Huaihe river line...
Muhammad Noor, Hidayat ur Rahman and Muhammad Iqbal
...ariability was mostly of genetic nature and hence, could be effectively utilized in maize breeding programs for developing popcorn varieties having early maturity and accompanied with other desirable attributes....
Urooj Fatima1, Arslan Mehboob2, Muhammad Abid3, Muti-ur-rehman Khan4 and Tahir Yaqub4*
...he current situation and genetics of CPVs in Pakistan, a total of fifty samples from sick dogs suspected for parvovirus infection were collected from different geographic areas of the country. Samples were screened for positivity and verified for CPV using PCR. From a 30% positive samples for hemagglutination, nineteen (38.4%) samples were positive in PCR. Sequence analysis of the selected positive samples revealed that both CPV-2 and new CPV-2a strains are ci...
K.N. Ahmed and M.R. Hasan
Sudden outbreak of mealy bug and armoured scale causing severe damage to economic crops in Bangladesh
... females develop parthenogenetically. It is a sucking pest and mainly feeds on citrus. Its secondary hosts include fig, apple, almond, guava etc. Due to severe infestation, premature falling of leaves occurs. The ber or plum scale, Aonidia ziziphi Rah. was recorded from Rajshahi, Bangladesh as a serious pest of ber or plum plant damaging the leaves and immature fruits during December-January, 2007. The female reproduces parthenogenetic...
Bijan Kumar Das
Incidence of Aleurocanthus spp. (Aleyrodidae: Hemiptera) on betelvine (Piper betle L.) and their interaction with host plants
...sts is very relevant for genetic improvement programmes. Till date, scanty information is available on the source of tolerance, if any, against betelvine blackfly. The reaction of some betelvine cultivars to A. rugosa was evaluated in the boroja with different varietal collections from different parts of India. None of the entries under the purview was completely free from infestation. A few cultivars [CARI- 2 (AN), CARI-6(AN) and Bilhari] exhibited moderate r...
M. S. A. Mamun and M. Ahmed
Integrated pest management in tea: prospects and future strategies in Bangladesh
...ltural control measures, genetic techniques and sex pheromones technology. In tea husbandry, cultural control measures such as plucking, pruning, shade regulation, field sanitation, fertilizer application, manipulation or destruction of alternate hosts and selection of pest resistant/tolerant varieties and mechanical mechanisms like manual removal, heat treatments, light traps, use of bio-pesticides, bio-control agents and sex pheromone trap need to be given m...
Rashid Pervez, Santhosh J. Eapen, S. Devasahayam and M. Dinsha
Characterization of entomopathogenic nematode, Stein-ernema carpocapsae from ginger (Zingiber officinale Rosc.) rhizosphere in India
...sequences analysis. Phylogenetic was constructed for studying relationship with known isolates. Pathogenic potential of the isolate of S. carpocapsae (IISR-EPN 06) on the larva of shoot borer (Conogethes punctiferalis), hairy caterpillar (Euproctis sp.) and greater wax moth (Galleria mellonella) was found under in vitro condition. Further this isolate displayed high virulence on above insect species. This study reported occurrence of a isolate of S. carpocapsa...
A. K. Ganguly and Uma Rao
Decades of researches in biochemical and molecular nematology at IARI
...d to study the molecular genetic diversity in major root knot and cyst nematode species of India to assist the breeding program for developing resistant varieties and other purposes like regulatory and nematode diagnostics. Attempts have also been to made to understand the molecular signaling events during compatible plant nematode interactions by identifying the genes during the transition from preparasitic to parasitic stage in ear cockle nematode and molya ...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
...s trigger the disease in genetically sensitive individuals. Gluten, a protein fraction commonly found in wheat grains, associated with food related disorders and a number of autoimmune diseases. We hypothesized that gluten containing diet would further exacerbate an already undergoing arbitrary immune reaction in SLE patients. Pristane was injected in female BALB/c mice to induce the disease. After five months, mice in various groups were treated with predniso...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1
...is a first report toward genetic screening of this gene at molecular level in Sahiwal cattle of Pakistan. No work has been reported on this gene in Sahiwal cattle. In this study, a new set of single nucleotide polymorphisms (SNPs) were reported. Heterozygosity, allelic and genotypic frequencies of identified variants were also determined. Chi2 test was performed to evaluate the Hardy-Weinberg Equilibrium of each polymorphic site. Two loci were found...
Ayesha Zahid,Ammara Muazzam, Sidra Mustafa, Saba Irshad*,Malik Siddique Mahmood and Rehman Shahzad
...ion in such patients but genetic researches have validated that, mutations in GJA8 are coherent source of lens opaqueness and inappropriate growth of fiber cells. In the present study, a novel G to C substitution (1104G>C) (pE368Q) was screened by PCR-SSCP in exon 2 of GJA8 and this tansversion altered exceedingly conserved glutamic acid to glutamine at site which was involved in coding of ASF1 like histone chaperone. Further presumption based on str...
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
...T34, S99 and P199). Phylogenetic analyses showed that the GPCR genes of LSDVs identified from ticks were closest genetically to the previously detected LSDVs from infected ruminants, indicating a potential role of Ixodid ticks for transmission of LSDV. This study showed the role of A. hebraeum ticks for transmission of LSDV. So, tick control is a crucial part, which should be included as a part of LSDV control measures in en...
Süleyman Kök*
... to compare 3 SNP of the genetic features of purebred TGC that are under the conservation of ex-situ (51 cattle) program with the ones raised by the smallholders (79 cattle) in the villages. According to the estimated average heterozygosity values for ex-situ breeding program and smallholders in the villages, the difference between the cattle were found meaningful (P<0.05). The observed average heterozygosity (Ho) value was calculat...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...enzymes with a wide phylogenetic distribution. They are multifunctional enzymes that play a crucial role in cellular detoxification and oxidative stress tolerance. Comparing with that in mammals, investigation of GSTs is more complicated in teleosts because of the greater pressure they suffer in aquatic environment. In this study, we identified a set of 27 GSTs including 8 classes of members in common carp genome. Both sequences alignment and phylo
Javeria1, Masroor Ellahi Babar2,*, Tanveer Hussain2, Rashid Saif2, Sadaf Rashid3, Hanan Sarfraz4, Abbas Ali Shah3, Muhammad Wasim1 and Muhammad Abdullah1
...udy is aimed to identify genetic variants in BDNF gene and their association with Obsessive Compulsive Disorder (OCD) among Pakistani Punjabi population. We recruited a total of 100 patients diagnosed with OCD following Diagnostic Statistical Manual-IV (DSM-IV) criteria and controls (n=120) were selected from the same ancestry during August 2011 to January 2014fromSir Ganga Ram Hospital and Panjab Institute of Mental Health, Lahore. The demographic valu...
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
...e to environmental or epigenetic effect, sampling number or a breed difference. To know the functional consequences of the identified polymorphism, RNA secondary structure analysis was performed and SNP was found changing the termination loop shape in mRNA. However, to increase the selection efficacy of dairy animals, the effects of candidate polymorphism should further be screened in different environments and genetic backg...
Muzafar Shah1,*, Mian Sayed Khan1, Muhammad Ather Rafi2, Habib Ahmed3 and James M. Carpenter4
...mily of Polistinae. Phylogenetic tree were reconstructed using 12 SrRNA (12S) gene showing homology of 73% compared with gene bank data while on the basis of cytochrome oxidase 1 (Co1) gene 93% homology were recorded. It was concluded that Co1 gene is more reliable than 12S rRNA gene for confirmation of R. brevita position in the Vespidae family.
...
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
Muhammad Imran Shabbir
...;">The heart of learning genetics is in realizing that how the likelihood of inheriting a particular trait can be predicted. This fundamental approach is the basis for plant and animal breeding to get desired varieties as well as performing genetic analysis including prediction of patterns of inheritance in family lines and to calculate the recurrence risk for relatives etc. Punnett square is used to describe the possible co...
Hassen Allaya1,2,*, Abderraouf Ben Faleh1, Manel Rebaya1, Sonia Zrelli3, Abdallah Hattour2, Jean-Pierre Quignard4 and Monia Trabelsi1
...caused by differences in genetic structure or environmental conditions.
...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
...for construction of phylogenetic tree and genetic diversity analysis. Lower genetic diversity was observed. The finding of this research is prerequisite for future research.
...
Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4
...ed to determine the phylogenetic relationship of currently prevailing H9N2 isolates in Pakistan. A sum of ten H9N2 AIVs were isolated from 400 suspected samples and were confirmed through serological and molecular assays. The sequence analysis of NA genes shown 99% homology with the H9N2 AIV recently isolated from Pakistan and their phylogenetic analysis revealed that all the isolates belonged to the G- lineage. Amino acid a...
Attaullah Khan1, Muhammad Shafi1*, Jehan Bakht2 and Shazma Anwar1
Fareeda Tasneem1, Farah Rauf Shakoori1 and Abdul Rauf Shakoori2,*
...pecies, a molecular phylogenetic analysis was performed on the basis of H4 gene sequences. A phylogenetic tree was constructed using the aligned sequences of our study with 22 entries of closely related species from GenBank Data. Highly polymorphic sites were observed in FT8 strain sequence as compared to other species. Analysis of H4 gene sequences in our study showed they are closely related and behaved as a good substitut...
Mehran Kausar1,2, Naveed Ashraf3, Farzana Hayat4, Asraf Hussain Hashmi1, Saima Siddiqi1,* and Mariam Anees2
...ycnodysostosis is a rare genetic bone disorder (OMIM 265800) with an autosomal recessive mode of inheritance. More than 50 mutations have been reported in CTSK (Cathepsin k) responsible for this disease. Mutations in CTSK result in impaired bone resorption consequently leading to short stature, increased bone density, recurrent fractures, stubby hands and feet with dystrophic nails, unossified fontanels, and an obtuse mandibular. The present stud...
Lu Liu1, Sher Khan Panhwar2, Tianxiang Gao3, Zhiqiang Han3, Chunhou Li4, Dianrong Sun4 and Na Song1,*
... The mean within-species genetic distances for L. affinis, L. carinata, and L. klunzingeri were 0.26%, 0.52%, and 0.47%, respectively. The mean genetic distances among L. affinis, L. carinata, and L. klunzingeri ranged from 12.22% to 14.74%, higher than the threshold for species delimitation. The results confirmed the taxonomic validity of the three Liza species and verified t...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,*
...In order to evaluate the genetic diversity and population structure for the conservation and management of sables (Martes zibellina), we developed six microsatellite markers using a method of fast isolation by AFLP of sequences containing repeats (FIASCO) from them in this study. The primers designed by Primer Premier 5.0 based on conservative regions were tested on 22 individuals. Our results demonstrated that the number of alleles per locus ranged fro...
Zuhao Huang*, Meifang Li, Rujue Ruan and Jiawei Qin
...llaris). The average genetic distances among the species varied from 5.39% (between Egretta sacra and Egretta garzetta) to 41.15% (between Egretta sacra and Botaurus stellaris). Five conserved sequence boxes (F to B) in the domain II of Ardeidae sequences were localized. Maximum likelihood method was used to reconstruct the phylogenetic trees based on HKY+ G model. The phylo
Javeria1, Masroor Ellahi Babar1,2,*, Akhtar Ali2, Asif Nadeem1, Abdul Wajid1, Sajjad Ali Shah3, Sadaf Rashid4, Muhammad Wasim1 and Muhammad Abdullah1
...tudyaimedto find out the genetic variations in Catechol O Methyl transferase (COMT) gene and association of these genes with obsessive compulsive disorder (OCD) in the Pakistani Patients. We selected OCD patients (n=100) following the Diagnostic Statistical Manual-IV (DSM-IV) criteria and controls (n=120) from Sir Ganga Ram Hospital and Panjab Institute of Mental Health, Lahore from August 2011 to January 2014. During the sample collection...
Mian Ahmad Raza*, Ghulam Hassan and Naqib Ullah Khan
Rabia Tahir1*, Khushi Muhammad1, Masood Rabbani1, Muti-u-Rehman Khan2, Farah Khan1, Hassan Bin Aslam1 and Arslan Mehboob3
...tions. However, based on genetic detection such evidences are lacking. The molecular mechanism and establishment of latent infections warrant future investigations to elucidate these discrepancies....
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...ed to LBA4404. The maize genetic transformation protocol developed in this study will possibly improve the efficiency to produce new transgenic maize lines expressing desirable agronomic characteristics. ...
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
... a starting material for genetic transformation. Unlike immature embryos, the development of coleoptilar node is seasonal free and green house independent which would accelerate the work relevant to genetic transformation of maize. MS medium fortified with 3 mgL-1 BAP and 5 mgL-1 picloram produced maize seedlings with expanded coleoptilar nodes. Longitudinal cut sections of expanded coleoptilar nodes produced maximum primary...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
...AR Lasergene. After phylogenetic analysis with 3 clonal types and atypical strains, and on the basis of restriction map of HhaI and Sau3AI, our 3 isolates of T. gondii were found more closely linked to a typical strain. This is the first genetic analysis of T. gondii in Pakistan. In order to develop our knowledge about the toxoplasmosis epidemiology, further genotype analyses of T. gondii from animals an...
Rabia Arif*, Shahar Bano, Muhammad Ishfaq and Muhammad Saleem
...fimicola, to examine genetic diversity. Strains were taken from two contrasting environments (Natural and Harsh) from the south facing and north facing slopes of Evolution Canyon, Israel. Sequenced amplicons were introduced to the NCBI database as a query in BLASTN. The query revealed >100% resemblance with the Ascomycota genus Sordaria and the topmost species in this investigation was fimicola. Circular Phylogram showed close relatedness ...
Mahmood-Ul-Hassan*, Naeem Fiaz, Muhammad Akhlaq Mudassir and Muhammad Yasin
...s for most of traits and genetic potential of genotypes. It was also found that the ratoon crop yield was highly dependent on the number of sprouts which were significantly varied among harvesting dates and the genotypes tested as well (R2=0.998 & 0.994, respectively).
...
Liana Mihaela Fericean1* and Mihaela Corneanu2
...phids gave birth parthenogenetically to three generations of fundatrigenous. In July, the winged form migrated to the secondary host, where successive generations have been born through parthenogenetic reproduction. Gynopara in October return to the primary host and give birth to wingless oviparae that mate with males and lay winter eggs. The biometrical measurements of Aphis nasturtii help to complete knowledge on ex...
Amjad Ali1*, Sher Aslam Khan2, Abid Farid2, Ayub khan2, Shah Masaud Khan2 and Naushad Ali2
...ability (repeatability), genetic gain and correlations among the parameters, and establish selection criteria.Highly significant (p ≤ 0.01) differences were found among the genotypes for number of tillers, plant height, cane length,number of nodes, internodes length,number of millablecane and cane yield while non-significant differences were recorded for cane diameter. The effect of genotype and crop interaction was highly significant fornumber of tillers w...
Estimation of Heritability, Genetic Advance and Correlation for Marphological Traits in Spring Wheat
Arshad Iqbal1*, Iftikhar Hussain Khalil1, Syed Mehar Ali Shah1 and Muhammad Sharif Kakar2
...gh heritability with low genetic advance was estimated for days to maturity, while moderate to high heritability coupled with genetic advance was recorded for other yield related traits. Correlation analysis showed that grain yield had strong positive phenotypic and genetic correlation with heading, maturity, flag leaf area and biological yield. Spring wheat genotype SM12 had best performa...
Tahira*, Muhammad Arshad, Muhammad Ayub Khan and Mubashar Ahmad Khan
...thod showed considerable genetic variation among the hybrids. In the first year, thirty- six Brassica hybrids were grouped into thirteen clusters. Cluster-IV comprised of maximum number of hybrids (six). The clusters mean and standard deviation marked that hybrid in Cluster XI (CRH-80) was short durational and high yielding mainly due to more branches and pods per plant.CRH-35 in cluster-VI was high yielding and has more seeds per pod. Similarly in second year...
Farid S. Ataya,1,2,* Dalia Fouad,3,4 Ajamaluddin Malik,1 Nikolaos E. Labrou,5 Mohamed S. Daoud1,6 and Hesham M. Saeed7
...an GSTP1-1 enzymes. Phylogenetic relationship was studied with different organisms belonging to animal kingdom and revealed that CdGSTP1-1 is grouped with the enzyme from S. scrofa. The 3D homology model of CdGSTP1-1 showed similar fold and topology with the porcine GSTpi enzyme. Gene expression analysis in five camel tissues was examined employing real-time PCR. The highest level of transcripts was found in the camel testis, followed by l...
Ehtisham Shakeel Khokhar1*, Amir Shakeel1, Muhammad Amir Maqbool1, Muhammad Waheed Anwar1, Zoraiz Tanveer1 and Muhammad Fahad Irfan2
...carried out to study the genetic variability, coefficient of variations, heritability, genetic advance and correlation among 20 F1 hybrids and 9 parents in upland cotton. Line × tester mating design was deployed to develop crosses and 29 cotton genotypes were field planted in randomized complete block design (RCBD) in three replications to assess the genotypes for plant height (cm), number of sympodial branches, number...
Ümit Kumbıçak, Zübeyde Kumbıçak* and Gülnare Huseynli
...karyotype.
...
Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*
...culated and computed for genetic associations presented as odds ratios (ORs) with a threshold of p≤0.05 to determine statistical significance. The frequency of 54Thr allele of FABP2 significantly associated with MetS risk in Pakistani population. Our results showed that frequency of the FABP2 54Thr allele was significantly higher (67%) in MetS cases as compared to controls (35%)(p<0.0001). The 54Thr allele carriers were at increased ...
Relationship of PIT-1 Gene Polymorphism with Breeding Parameters and Body Weights of Cows and Calves
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
...-Fresian cattle there is genetic variety in analysed locus c.1178G>A gene PIT-1. Frequency of genotypes and alleles was respectively: AA=0.0469, AG=0.3154, GG=0.6377, A = 0.2046 and G = 0.7954. AA homozygotes had the highest age at first insemination and the lowest insemination index. Cows with this genotype after calving and calves (both gender) from AA homozygotes had the biggest weight after calving. In contrast GG homozygotes had th...
Rafiullah*, Muhammad Jamal Khan and Dost Muhammad
Warda Fatima1 , Shahida Hasnain1 , Saqib Mahmood2
...hether it’s a pure genetic disease. It is considered a polygenic multifactorial condition where both genes and environment have roles in disease development. In the present study we selected a family withmultiple schizophrenic patients and have identified some reasons for disease development. It is estimated from study that in Pakistan there is gender bias in schizophrenia unlike the worldwide data because more of males are affected in the selected famil...
Yunjun Yan, Zhenming Lü, Tianming Wang, Yongjiu Chen, Jingwen Yang, Baoying Guo, Lihua Jiang, Changwen Wu and Liqin Liu*
...a, and analyzed the phylogenetic relationship with other cephalopod species. The results show that the mitochondrial genome of O. dollfusi composed of 15843 nucleotide pairs and encodes 13 proteins, 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs) and a major long noncoding region (LNCR) of the mitochondrion’s own protein synthesizing system. Seven of thirteen proteins, eight tRNAs are encoded by the plus strand, while the other proteins and tRN...
Tania A. Shakoori1,*, Soumble Zulfiqar2, Khadija Irfan Khawaja3 and Faisal Masud4
...ot reported. We compared genetic expression of Sirt1, AMPK, Akt, and mTOR in adipose tissue from mice treated with NaB versus placebo. Four groups of mice (n=27) mice were administered oral NaB, intraperitoneal (i.p) NaB, oral placebo or i.p placebo for 16 days. RNA was isolated from the epididymal fat pads and relative genetic expression of AMPK, Sirt1, mTOR and Akt was measured using real time PCR. Oral NaB group showed de...
Syed Asif Imran Shah1*, Shah Jehan Khan2, Kalim Ullah1 and Obaid Ullah Sayal2
...es were investigated for genetic divergence regarding yield related attributes, seed cotton yield and fibre quality traits using principle component analysis. The experiment was carried out at Cotton Research Station, Deara Ismail Khan, Pakistan during 2014-15. The triplicated randomized complete block design was followed and attributes of interest were plant height, monopodia plant-1, sympodia plant-1, bolls plant-1, plant population, seed index, boll weight,...
Muhammad Abubakar,1,2,* Aamer Bin Zahur,1,3 Khalid Naeem1,3, Muhammad Azeem Khan1,3 and Subhan Qureshi4
... The results of the phylogenetic tree indicated that all Pakistani PPRV strains, regardless of the gene used either F or N, clustered in lineage IV which is the most prominent and prevalent lineage of Asia. The distribution of Pakistani strains of PPRV was more dispersed as the isolate collected from Taxilla was clustered slightly distinct compared to rest of the isolates collected from Pakistan. Our findings are indicative of PPR endemic state of the country....
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
...was planned to study the genetic diversity in the M. tuberculosis isolates isolated from clinical samples. In the current study total 794 patients were tested for suspected tuberculosis by fluorescence microscopy and GeneXpert system during the study period (2015-2016) at the Provincial TB Reference Laboratory, Khyber Pakhtunkhwa, Pakistan. Among the 794 patients studied, 170 samples gave positive results. Fifty-two Mycobacterium tuberculosis com...
Zahid Akram, Saif Ullah Ajmal, Ghulam Shabbir, Muhammad Munir and Nasir Mahmood Cheema
INHERITANCE MECHANISM OF SOME YIELD COMPONENTS IN BREAD WHEAT
...alysis. The estimates of genetic component of variation in all the traits exhibited the preponderance of
over dominance gene action. The estimates of average degree of dominance for spike length (1.139), 1000 grain weight (1.421), number of spikelets spike-1 (2.293), number of grains spike-1 (1.631) and grain yield plant-1 (1.111) evidenced the prevalence of non additive gene action controlling these traits. The present results, therefore, revealed...
Raania Ahsan* and Zafar Altaf**
...an to introduce advanced genetically modified (GM) crop technology in Pakistan by Government of Pakistan during 2009. In this regard Ministry of Food and Agriculture (MINFA) has been working on a two pronged strategy i.e. developing the technology through indigenous capabilities as well as inviting the multi-national companies (MNCs) to bring in the latest cotton production and protection in the country. Bt cotton can play a significant role to enhance agricul...
Z. A. Soomro, M.B. Kumbhar, A.S. Larik*, M. Imran** and S.A. Brohi***
...nbsp;Heritability (b.s), genetic advance, phenotypic and genotypic variance for different quantitative traits is important in determining the effectiveness of selection. In this study the potential effectiveness of selection in parents and their F2 and F3 progenies of Gossypium hirsutum for plant height, sympodia/ plant, bolls/plant, boll weight, seed index and seed cotton yield/plant was determined. It was found that all the populations alongwith parents diff...
Oguz Bilgin, Kayihan Z. Korkut, Alpay Balkan, and Ismet Baser*
...amme. Highly significant genetic variability was present in parents and their genetic combinations for the characters. The degree and direction of heterosis varied for different characters and for different hybrids. The highest heterosis over mid and better parents (120.14-109.93%) was recorded in hybrid of Kyzyltan91xIDSN209 for grain weight/spike. Maximum limits of heterosis over mid and better parent for spike length, spi...
M. Yasin Mirza, Mubashir A. Khan, M. Akmal, Akbar S. Mohmand, Malik S. Nawaz, Nazakat Nawaz and Najeeb Ullah*
...onding high estimates of genetic advance in percent of mean (17.23 and 18.33) for these traits indicated the predominance of additive gene effects. Mass selection based on these additively controlled traits is suggested for improvement of yield. Plant height, number of bolls plant-1 and 1000-seed weight showed genetic advance of 7.96, 8.85 and 17.23 % respectively with the prospects of improving seed yield up to the level of...
Shazia Erum*, Muhammad Naeemullah, Shahid Masood** and Muhammad Irfan Khan*
...s of total seed proteins genetic distances ranged from 0.11 to 1.00. Due to greater genetic diversity in Ocimum germplasm and its suitability for commercial cultivation even in the area under small holdings, the investigation suggest its genetic as well as biochemical investigation for the production of commercial varieties and exploitation of the plant for economic benefits of the local c...
Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2*
...These findings show some genetic features of CCoV in Brazil and may require future studies to elucidate full genome sequences of these isolates to better assess the disease transmission dynamics and future control strategies. ...
Saba Rafique1,2,*, Khalid Naeem1, Naila Siddique1, Muhammad Athar Abbas1, Aamer Ali Shah2, Akbar Ali1, Abdul Rahim1 and Farooq Rashid1
...on number KU145467. Phylogenetic grouping and maximum nucleotide sequence identity values were used to identify the isolate that looked to be derived from recombination. It showed maximum nucleotide homology 99.5% with ck/CH/LHB/121010 (KP036503), India/IBV572 (KF809797) and Japan/JP/Wakayama-2/2004 (AB363951.2) and 99.3% with 4/91 vaccine (KF377577), Iran/491/08 (HQ842715) and 99.1% with India/NMK/72/VRI/10 (HM748585) and was least related to rest of IBV line...
Haixia Liu, Yuwei Ye, Yang Li, Xiaolin Liu, Dongmei Xiong* and Lixin Wang
...m the two rivers and the genetic diversity of wild population, 11 microsatellite loci were firstly used to evaluate the genetic characterization using 120 samples from 2 tributaries (ZZ and LX populations) of the Weihe river and 2 tributaries (YX and TB population) of Hanjiang river. A total of 59 alleles were found and the number of alleles in each locus ranged from 3 to 8, with an average of 5.4. There were 6 highly polymo...
Muhammad Rasool1,*, Muhammad Zahid1, Muhammad Ismail Khan1, Qadeem Khan2 and Khalid Khan1
...no acid change. The phylogenetic studies established that the Pakistanian HBV Isolates closely resemble the “adrq” strain belonging to “adr” subtype. The study showed that the pre-S2 region is hypervariable and two reported sequences show 100% homology and thus cause change in the structure of Hepatitis B Surface Antigen (HBsAg). This variability reduces the efficacy of vaccines prepared to a particular clone of HBV isolates.
...
Lin Wang1,2, Ye Gong3, Kelin Chen1, Haitao Wang3 and Xianguo Lyu1,*
...nce our understanding of genetic information and breeding biology of Relict Gull.
...
Muhammad Yaqoob*, Rukhsana Anjum, Mumtaz Hussain and Muhammad Jahangir Shah**
GENETIC DIVERSITY ANALYSIS AND CHARACTER ASSOCIATION IN SOME CHINESE HYBRID RICE UNDER DRY CONDITIONS
...ndicated a high range of genetic variation among various hybrids for plant height, number of grains per panicle, number of productive tillers per plant, 1000 grain weight and paddy yield. The variation for days to 50% panicle initiation, panicle length, spikelet fertility and days to maturity were however nonsignificant. Plant height ranged from 77 to 116 cm, number of grains per panicle from 152 to 449, number of productive tillers per plant from 15 to 29, 10...
Muhammad Yasin Mirza*, Mubashir Ahmad Khan*, Muhammad Amjad* and Malik Shah Nawaz*
STABILITY ANALYSIS FOR ECONOMIC TRAITS IN SESAME ( L.)
...e of genotypes was under genetic control. The b-values of V-90005, T-89 and PARS-I were larger than unit regression; hence were suitable for favourable environments for yield. Whereas, V-III and SangharI were with b-values less than unity indicating their below average response. For branches per plant, Sanghar-1 and S-17 had regression coefficients less than one with negative sign making them suitable only for poor environment. Four genotypes namely, Sanghar-I...
Mubashir Ahmad Khan*, Muhammad Yasin Mirza*, Muhammad Amjad*, Nazakat Nawaz*, Malik Shah Nawaz* and Doulat Baig*
ASSESSMENT OF GENETIC DIVERSITY IN GERMPLASM OF LINSEED ( L.)
...the existence of greater genetic diversity in the accessions for these traits. Dendrogram based on Euclidean distance coefficient using 10 quantitative traits, grouped all the linseed accessions into 13 clusters. Cluster II was the biggest and had 33 accessions followed by Cluster I having 11 accessions. For the development of high yielding varieties, best performing accessions of Clusters I and II could be used in hybridization programme by crossing with acce...
Said Salman*, Shah Jehan Khan*, Javed Khan**, Rehmat Ullah Khan*** and Imran Khan****
GENETIC VARIABILITY STUDIES IN BREAD WHEAT (TRITICUM AESTIVUM L.) ACCESSIONS
...y estimates and expected genetic advance were found for all the traits except chlorophyll concentration index, spike length and number of -1 spikelet spike which exhibited moderate heritability. Based on Euclidian dissimilarity distance, 65 wheat accessions were classified in to 6 different clusters. Maximum diversity was found in cluster 1 and cluster 4. This maximum diversity explains the better parental selection for future breeding programme....
Abdul Hassan*, Muhammad Ishaq*, Nisar Ali Shah* and Arshad Farooq*
MILK PRODUCTION POTENTIAL IN KHYBER PAKHTUNKHWA
...by proper management and genetic improvement. More research is needed at farm level in the context of breed adaptability and management in respect of cost of livestock herds production in Khyber Pakhtunkhwa, Pakistan....
Muhammad Sohail*, Imtiaz Hussain*, Riaz-ud-Din*, Sikandar Khan Tanveer*, Maqsood Qamar* and Syed Haider Abbas*
EVALUATION OF ADVANCE WHEAT LINES FOR AGRONOMIC TRAITS IN RAINFED ENVIRONMENT
...397 and NARC-09 have the genetic potential to tolerate adverse rainfed growing conditions under agro-ecological conditions of Pothwar region, Pakistan....
Frasat Saeed*, Jehanzeb Farooq*, Abid Mahmood**, Tassawar Hussain***, Muhammad Riaz* and Saghir Ahmad****
GENETIC DIVERSITY IN UPLAND COTTON FOR COTTON LEAF CURL VIRUS DISEASE, EARLINESS AND FIBER QUALITY
...LCuD). For estimation of genetic diversity regarding CLCuD tolerance, fiber quality and some yield contributing traits, 101 cotton genotypes imported from USA were evaluated. Different statistical procedures like cluster, principle components (PC) and correlation analysis were employed to identify the suitable genotypes that can be further exploited in breeding programme. Significant associations were found between yield contributing trait, boll weight and fib...
Rashid Minhas*, Iftikhar Ahmad Khan**, Faisal Saeed Awan**, Lal Hussain Akhtar*, Syed Awais Sajid Shah* and M. Shahjhan Bukhari*
ESTIMATION OF GENETIC DIVERSITY AND HYBRID IDENTIFICATION IN AMERICAN COTTON (GOSSYPIUM HIRSUTUM L.) BY PCR BASED RAPD ANALYSIS
...ure leaves to estimate 1 genetic diversity and hybrid identification. Out of the 26 RAPD primers used for DNA amplification, 14 primers produced polymorphic bands, while 12 produced monomorphic bands. These 14 primers yielded total 96 RAPD markers, out of which only 40 were found polymorphic which resulted in 41.66% polymorphism among the genotypes studied. The number of RAPD markers produced ranged from 4 to 12. Average number of 6.40 bands per primer was obs...
Kalim Ullah*, Zahid Usman*,Niamatullah Khan*, Rehmat Ullah**, Fazal Yazdan Saleem***, Shahid Iqbal Khattak**** and Muhammad Ali*****
GENETIC DIVERSITY FOR YIELD AND RELATED TRAITS IN UPLAND COTTON GENOTYPES
...periment to evaluate the genetic variability and yield potential in cotton cultivars was conducted at the Cotton Research Station, D.I.Khan, Pakistan, during 2013. The experiment was carried out in Randomized Complete Block design replicated four times. The genetic material comprised nine cotton cultivars viz., Gomal-93, SLH-373, BH-186, BH-172, CIM-573, CRIS- 342, CIM-591, CIM-608 and DNH-105. Results depicted the significa...
Israr Ali*, Naushad Ali*, Riffat Tahira**, Sardar Ali*, Izhar Hussain* and Sher Aslam Khan*
GENETIC DIVERSITY ASSESSMENT IN BRASSICA GERMPLASM BASED ON MORPHOLOGICAL ATTRIBUTES
...es that this genotype is genetically different and superior than the other studied genotype. Therefore, genotype Kalabat can be either used as variety after adaptability trials over a larger area or included in Brassica breeding programmes as a good source of genetic variation. ...
Muhammad Mohsin Raza*, Muhammad Aslam Khan*, Irfan Ahmad**, Ali Ahsan Bajwa***, Hafiz Muhammad Usman Aslam*, Badar Ahsan Ullah****, and Kashif Riaz*
FOREST PATHOGENS AND DISEASES UNDER CHANGING CLIMATE -A REVIEW
..., risk rating, planning, genetic diversity and facilitated migration, genetic protection and breeding for disease resistance and relating results to forest policy, planning as well as decision making, the suspicions innate to climate change effects can be minimized....
Sohaib Arshad*, Sarwat Naz Mirza*, Imtiaz Ahmad Qamar** and Maqbool Shahbaz**
DETERMINATION OF FORAGE QUALITY OF INDIGENOUS AND EXOTIC RHODES GRASS ACCESSIONS UNDER RAINFED CONDITIONS
...d be attributed to their genetic inheritance potential. Mean value of crude protein content (CP) in three grasses varied from > 12% to < 5% depending upon the different growth stages of the grasses. Sabre showed highest CP content than other two at flowering stage. The results also showed that protein contents were lowest at the maturity stage of all the experimented grasses. This study has shown that Katambora is the variety which can be adapted to Paki...
Basharat Hussain Shah*, F. S. Hamid*, Shams ul Islam*, Fayaz Ahmad*, Sohail Aslam*, and Noorullah Khan*
EVALUATION OF DIFFERENT PEA (PISUM SATIVUM L.) GENOTYPES FOR YIELD AND OTHER ATTRIBUTES AT SHINKIARI, MANSEHRA
...o find out the amount of genetic variability that may exist in different pea cultivars. For this purpose six advance lines/commercial pea varieties were collected from Ayub Agricultural Research Institute (AARI) Faisalabad and one local variety was included in the experiment as a control. The experiment was conducted during 2014-15 at National Tea & High Value Crops Research Institute (NTHRI) Shinkiari, Mansehra. Altogether seven pea genotypes were laid ou...
Nasarullah*, Raiz uddin*, Fakhar Uddin* and Muhammad Jamal*
INTERPRETING WHEAT GENOTYPES FOR GRAIN YIELD UNDER DIFFERENT REGIMES OF KHYBER PAKHTUNKHWA
... three replications. The genetic material was comprised of 9 nationally released cultivars, three advance line from Tajikistan, one from China and two locally grown cultivars. The analysis of variance revealed significant differences among genotypes, locations and genotype by environment interactions for days to heading, days to maturity, plant height (cm), spike length (cm), spikelets spike , 1000-grain weight and grain yield. Flag leaf area (cm displayed non...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
...A, S336N). The HA and NA genetic evolution analysis revealed that one isolate was most similar to the newly isolated H3N2 viruses from dogs in China and had the same evolutionary branching. The result provided a foundation for further studies on biological characteristics of CIV.
...
Yanling Xia1,2, Heping Li2, Yuntao Liang2, Jichen Zhao2, Binshan Lu2 and Di Liu1,*
...uence alignment and phylogenetic tree analysis indicated that theVps26A protein of sika deer was highly similar to that of Bos taurus. Expression analysis by real-time quantitative RT-PCR revealed that Vps26A gene had a higher expression level at day 90 than those obtained at day 60 and 30.
...
Ali Haider Saleem1,*, Khalid Javed2, Masroor Ellahi Babar3, Tanveer Hussain3, Asad Ali2, Afzal Ali2, Nisar Ahmad2, Muhammad Zahid Farooq1 and Muhammad Dawood2
...onfirmed the presence of genetic variability among Lohi animals; this variation might be associated with other economic traits for future breeding programs and marker assisted selection.
...
Sana Zahra
... of Pakistan. A detailed genetic evaluation of four unrelated but ethnically similar families from Punjab province was performed.Linkage to SLC26A4 locus was confirmed through linkage analysis using Short Tandem Repeat Markers (D7S2420, D7S2459 and D7S2456). p.Y556C was identified as causative mutation by Sanger Sequencing. A known missense mutation p.Y556C in exon 15 of SLC26A4 gene was identified in all the four families. It may be concluded th...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
...assaemia is an inherited genetic disorder of haemoglobin. It is estimated that about 100,000 patients are presently suffering from thalassaemia major, the severe form of the disorder. The patients of β-thalassaemia are dependent upon lifelong blood transfusion. Multiple transfusions expose them to many blood borne diseases, most commonly hepatitis B and C. The aim of current study was to determine the prevalence of HBV and HCV infections among thalassaemi...
Ehtisham Shakeel Khokhar*1, Amir Shakeel1, Muhammad Amir Maqbool1, Muhammad Khubaib Abuzar2, Sumaira Zareen2, Syeda Sana Aamir4 and Muhammad Asadullah4
...dicating the presence of genetic variability except fiber fineness. δ2GCA/δ2SCA ratio depicted the predominance of non-additive types of gene action for plant height, number of monopodial branches, number of sympodial branches, numbers of bolls, boll weight, seed cotton yield, lint percentage, fiber length and fiber strength. The parents VH-259, CRS-456 and AA-703 among lines and PB-39 among testers displayed significant GCA e...
Ambreen Asghar, Tasleem Akhtar, Nadeem Sheikh*
...and obesity. Despite the genetic regulation of metabolic responses, a close relationship between high energy food-intake and increased body-weight has been reported to support several pathologies in the metabolic and immune system. Present work was designed to evaluate the variations in serum protein level induced by fat rich diet. For this purpose, two groups of Wister rats were fed with diets carrying difference in percentage of fats. Protein profiling of tr...
Ligia Neves Scuarcialupi, Laila Andreia Rodrigues Beserra, Julia Rosas Hochheim, Rodrigo Martins Soares, Fabio Gregori*
...ere further submitted to genetic sequencing and analyzed by phylogenetic analysis. There were 5 RVF positive samples (5.15%) in 2 broilers, 1 laying hen and 2 breeders. Only one RVG sample (1.03%) was detected in laying hen, in coinfection with RVF. This data contributes to a better understanding of rotavirus distribution in Brazil.
...
Evolutionary Analysis and Phylodynamics of Avian Influenza Virus H5N1 between 2015 and 2016 in Egypt
Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*
...sified into two distinct genetic subclades (2.2.1.1 and 2.2.1.2) in Egypt. This study was conducted as part of routine surveillance activities in Egypt; 5202 cloacal and oropharyngeal swabs were collected from live/dead birds, including commercial and backyard flocks, in five different locations in Egypt between the years of 2015 and 2016. All samples were screened using virus isolation, as well as antigenic and molecular detection methods. Full-length hemaggl...
Shuai Shang1,2, Honghai Zhang2,*, Xiaoyang Wu2, Qinguo Wei2, Jun Chen1, Huanxin Zhang1, Huaming Zhong2 and Xuexi Tang1,*
...n, we constructed a phylogenetic tree using Tas2r genes sequences from these fifteen species. Phylogenetic analyses showed that most Tas2r genes of raccoon dogs were closed to other canid-species, indicating that the Tas2r genes were very conservative. Besides, we also detected positive selection in 11 intact genes in raccoon dogs. The results of the positive selection analyses indicated that Tas2r10<...
Sher Nawab Khan* and Ghulam Hassan
Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1
... there were considerable genetic differences among the Trichoderma isolates. While, there was no correlation, found between RAPD clusters and aggressive groups (AGs). Isolates belonging to different AGs were found scattered among the four RAPD clusters. It was concluded that the indigenous isolates of Trichoderma harzianum were found effective against root knot nematodes and there was a significant difference among these isolates, at the molecular level. ...
Aqeela Ashraf1,2,*, Muhammad Imran1 and Yung-Fu Chang2
...industry due to its high genetic variability, enormous number of environmental sources and increased resistance to antibiotics. The present study was aimed to determine the antimicrobial resistance of E. coli isolates from bovine mastitis and identification of antimicrobial resistance and virulence genes associated with them. Antimicrobial susceptibility testing was performed by disk diffusion method. Resistance and virulence genes were detected by PCR....
Baoying Guo*1, Yu Chen1, Chuan Zhang2, Zhenming Lv1, Kaida Xu3, Hongling Ping4 and Huilai Shi4
...es, and constructed phylogenetic trees estimating their relationships. The genome was 16,228 bp and contained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 2 long-noncoding regions [both in the control regions (CR)]. The composition and order of genes in S. lycidas were similar to those of most other invertebrates. The overall base composition of S. lycidas is 35.8% T, 14.8% C, 41.3% A, and 8.1% G, with extremely high...
Zeqin Fu, Yunfang Tian, Yingying Ye*, Pengzhi Qi and Changwen Wu
...o choose for researching genetic diversity and population structure and provide more in-depth understanding of Mytilus coruscus.
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Jun Chen1, Shuai Shang2, Xiaoyang Wu1, Jiakuo Yan1, Huaming Zhong1, Huanxin Zhang2, Weilai Sha1, Wanchao Zhu1 and Honghai Zhang1,*
...k offers an initial phylogenetic baseline for further research on the intestinal ecosystem of these African animals. This work is of great significance for disease monitoring and protection of these animals.
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