Felwa A. Thagfan1, Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1
.../i> induced infection in mice jejunum. Three different doses, 300, 600 and 900 mg/kg were used after infection of mice with sporulated oocysts. A dose of 300 mg/kg reduced the number of oocyst output in mice faeces by about 56.8 % and also improved the body weight. In addition, the root extract decreased the number of parasitic stages and the number of goblet cells in the jejunal villi in ...
Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*
... total of 30 female BWF1 mice were randomly divided into three groups as follows: group (I) control group (lupus uninfected); group (II) live P. chabaudi infected group (lupus + live P. chabaudi infection); and group (III) irradiated P. chabaudi-infected group (lupus + irradiated P. chabaudi-infection). All groups were killed at day 14 post infection. Histological and biochemical investigations were performed.Live P. chabaudi infection was accompanied with an ...
Faiqah Ramzan*1 and Muhammad Haris Ramzan2
...tric acid levels in male mice. Kisspeptin-10 was administered intraperitoneally in different dosage concentrations (1μg, 1 ng, and 10 ρg) to adult male mice twice daily for 12 days. Prostatic citric acid levels were determined photometrically. The levels decreased significantly at all tested doses. Intermittent administration of Kisspeptin –10 negatively regulates the prostatic citric acid levels in adult male <...
Jixue Hou1,2, Xueling Chen3, Jing Wang4, Hongwei Zhang2, Yu Xi2, Jie Xia2, Xinyu Peng2,* and Xiangwei Wu2,5*
...bbits, and big rats. The mice with reproduction fast cycle, lower costing, and close to human genes, can be showed about some phenomenon quickly, also more easy to form model of atherosclerosis. Thus, a mouse model of vascular injury is vitally important for researching the pathophysiological mechanisms to restenosis after percutaneous transluminal coronary angioplasty (PTCA) as well as translational approaches. In this Experiment, we established a unique muri...
Tianzhu Chao, Huiqiang Cai, Yuxun Zhou, Kai Li* and Junhua Xiao
...enetics of Chinese house mice based on 466 DNA sequences from mitochondrial D-loop fragments. Our analyses revealed that Mus musculus musculus and M. m. castaneus are older colonized subspecies with broad distributions in East China, while M. m. domestics is a newly arrived subspecies currently restricted to Shanghai, indicating the elevated risk in this cosmopolitan cities. Phylogenetic analysis indicates that M. m. musculus migrat...
Yi-qiang Ouyang1, Zhen-xin Liang1, Shi-wen Huang2, Ying Zhang3, Shao-shi Luo1, Jin-ning Liang1, Jia-fu Li1, Yu He4 and Song-chao Guo1,*
...se (AD) involve rats and mice, yet the tree shrew (Tupaia belangeri) is in many respects more similar to primates than rodents are. To demonstrate the feasibility and usefulness of using the tree shrew as an AD model, we examined how well the model recapitulates features of the disease. Injecting animals intraperitoneally with d-galactose to induce acute senility, and then injecting them with β amyloid fragment 1-42 and ibotenic acid into the bilat...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
...njected in female BALB/c mice to induce the disease. After five months, mice in various groups were treated with prednisone and fed with gluten containing and standard diet for four weeks and applied procedure to detect minor changes in paw swelling, ANA autoantibodies, CCL11, C3c, glucose level and renal damage. We detected increased symptoms of arthritis and gastrointestinal tract involvement in gluten containing diet grou...
Madeeha Arshad1, Naveed Ahmad2,*, Muhammad Khalid3, Asmatullah1, Mohammad Tahir2, Khadija Naveed1 and Asia Iqbal4
...administered to pregnant mice. LD50 of Cr VI, was calculated as 88mg/kg of B.W in pregnant mice. Among 05 groups of pregnant females (10 females in each group), three were treated with 50%, 25% and 12.5% of the LD50 of Cr VI, respectively, with one control and one vehicle control group. Fetuses were recovered on 18th day of gestation and fixed for morphometric and morphological studies. Exper...
Fariha Imtiaz1, Muhammad Islam1, Hamid Saeed2,*, Bushra Saleem1, Maryam Asghar1 and Zikria Saleem2
...xtracts and minoxidil on mice hair diameter. Taken together, our data suggested that ethanol extracts of leaves of T. foenum-graecum had significantly higher growth promoting effects compared to standard, i.e., minoxidil. Thus, results from our study set the stage of further studies to identify the constituents in ethanol extracts, responsible for hair growth promoting effects.
...
Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
...vival in macrophages and mice than the parent strain 2308. With decrease in the spleen/body weight ratios of mice infected with omp10 mutants were noted, inhibition of phagosome-Lysosome fusion was dramaticly impaired, and profound increase of apoptosis rate after exposure of RAW264.7 to omp10 mutants at 12 h, 24 h and 48 h compared to RAW264.7 exposed to 2308. Results indicate that Δomp10 mutant w...
Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3*
...in xenotransplanted SCID mice and by histopathological and immunohistochemical analyses. Our results demonstrated that fusion protein VEGFR1(D1-D3)-Fc efficiently inhibited
the lung cancer growth in vivo. Although the aptamer RNV66 was not found to be as efficient in this study, the results were also not surprising as the aptamer was administered naked and at low dose. As these are only our preliminary studies, a detailed investigation is planned using ...
the lung cancer growth in vivo. Although the aptamer RNV66 was not found to be as efficient in this study, the results were also not surprising as the aptamer was administered naked and at low dose. As these are only our preliminary studies, a detailed investigation is planned using ...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2
...inistered to male albino mice for up to eight weeks for growth promoting activity. Increased mammary growth was observed in test female non lactating mice, as compared to controls when placental lactogen was applied. Increase weight gain for visceral tissues like kidney, liver, muscle and lungs was also observed, as compared to control subjects. It was also recorded that the exogenous supply of bubaline PL reduces fat cells ...
Rabia Qurashi, Qurat ul Ane Gillani* and Furhan Iqbal*
...f 6 week old male albino mice. Blood samples from male albino mice (CGP55845 treated N = 14 and saline treated N = 16) were collected directly by cardiac puncture and used for estimation of hematological and some serum biochemical parameters. The hematological parameters did not show any significant variation between the two groups. From amongst serum biochemical parameters triglycerides (P = 0.02) in CGP55845 treated
Abd El-Nasser Ahmed Mohammed*
... upon transplantation in mice and rats. Eighteen adult female rats were classified to three equal groups in addition to sex young females as donor of young ovaries. Similar classification was done using eighteen female mice and sex young females. The females of mice or rats were anesthetized for ovarian transplantation using (6.2 and 13.2 mg/kg BW) and (13.3 and 26.6 mg/kg BW) diazepam and...
Tania A. Shakoori1,*, Soumble Zulfiqar2, Khadija Irfan Khawaja3 and Faisal Masud4
...R in adipose tissue from mice treated with NaB versus placebo. Four groups of mice (n=27) mice were administered oral NaB, intraperitoneal (i.p) NaB, oral placebo or i.p placebo for 16 days. RNA was isolated from the epididymal fat pads and relative genetic expression of AMPK, Sirt1, mTOR and Akt was measured using real time PCR. Oral NaB group showed decreased genetic expression of AMPK (...
Asmatullah1, Chaman Ara1,*, Shagufta Andleeb2, Maha Tahir1, Beenish Zahid1 and Madeeha Arshad1
...by cadmium in developing mice. For this purpose, different aqueous concentrations of cadmium chloride i.e. 0.00, 6.25, 12.50, 25.00 µg/g B.W of mice were given orally to pregnant mice on days 6-12 of gestation daily. Another series of mice were treated with different concentrations of cadmium chloride and also with an antidote (guava fruit e...
Basit Zeshan1,2,*, Mushtaq A. Saleem2,Javed Iqbal Wattoo2, Mohd Mokhtar Arshad1 and Maizan Mohamed1
... an animal experiment on mice, in which mice were injected subcutaneously. These findings suggest that the truncated Sf200 expressed in the pET-32a (+) prokaryotic vector can be used as antigen to detect antibodies against IBV.
...
Kaori Sasaoka1, Takahiro Kataoka1, Norie Kanzaki1, Yusuke Kobashi1, Akihiro Sakoda2, Yuu Ishimori2 and Kiyonori Yamaoka1,*
...or highly radiosensitive mice. CDDP was administered at 20 mg/kg weight to C57BL/6J and BALB/c mice after radon inhalation at 1000 Bq/m3 and 2000 Bq/m3 for 24 h. Radon inhalation had a slight positive effect against CDDP toxicity in C57BL/6J mice with respect to improved hair condition, whereas radon inhalation exacerbated CDDP-induced toxicity in BALB/c
Muhammad Nazar Aftab1, Ahmed Ali1, Muhammad Asad1, Sadia Fatima2 and Furhan Iqbal2,*
...l profile of male albino mice. Eight week old male albino mice were used as experimental animals and were divided into two groups. First group was treated with 80mg/Kg body weight of AlCl3 for 16 days while control group was treated with saline solution for the same period of time. Complete blood count were determined in both experimental treatments at the end of dose supplementation. Our results revealed that ora...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...sequently, thirty female mice randomly were divided into three groups, treating with 50 μg pEGS/2SS-tPA (group T1), 50 μg pEGS/2SS (group T2), and 50 μg pEGFP-N1 (Control group) at weeks 0, 2, 4 and 6. Mating with male mice was performed at 2 weeks post final immunization. Results showed that mice both in T1 and T2 groups elicited significantly stronger anti-SS antibody level than...
Madeeha Arshad1, Asmatullah1, Chaman Ara1, Shagufta Andleeb2 and Naveed Ahmad3,*
...developmental defects in mice fetuses. Different concentrations of the drug, 0.00, 4.10, 8.33 and 16.66 µg/g B.W. were administered orally to the dams on days 6-12 of gestation, and fetuses were recovered on day 18. Morphological observations of fetuses from 4.10, 8.33 and 16.66 µg/g B.W. dose groups showed abnormalities such as skin hemorrhages, microphthalmia, limb deformities, hygromas, kyphosis, curved and short tail and fluid filled abdominal ...
Zain ul Abidin1, Aisha Khatoon2, Abdul Whab Manzoor1,*, Nida Arooj1, Sajjad Ali1 and Muhammad Numan1
...legs were noticed in the mice of both groups between 11-15 days post inoculation while all the mice were found dead between 15-18 days post inoculation in mouse inoculation test (MIT). Reverse transcriptase-polymerase chain reaction (RT-PCR) performed for both samples gave a product of 443-bp amplifying the highly conserved “N”-region gene of virus confirming the rabies infection in both cases.
...
Imran Taj1,*, Ferhat Abbas1, Zafar Ahmad1, Mohammad Kamran Taj1, Deedar Ahmad2, Zahoor Ahmed2, Ghulam Mohammad2, Ajaz-ul-Haq1, Farooq Shahzad1 and Sabiha Azam3
...reas and gall bladder of mice.
... 
Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2
Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
...c anomalies. Male albino mice (Mus musculus) were equally divided (n=10) as C; control, Cr-treatedandCr-JgroupsreceivingCr+6 in the form of potassium dichromate (K2Cr2O7) 50ppm for 10days ad-libitum while Cr-J group additionally given 0.25ml/12h Jambul Fruit Extract (JFE) for next 5days by oral gavage. On the 16th day blood, liver and kidney were collected for biochemical and histopathological analysis.Cr+6 treated group showed severe histological changes like...
Muhammad Mohsin Ahsan1, Hafiz Muhammad Tahir2, Muhammad Khalid Mukhtar1
Effect of lunar cycle on active population density of scorpions, their potential prey and predators
...ws, lizards, centipedes, mice and rats) was noted in the presence or absence of moonlight, but they were
observed to be more active during the moonlight period than during moonless nights....
observed to be more active during the moonlight period than during moonless nights....
Muddasir Hassan Abbasi1, 2, Noor Fatima1, Syed Shahid Imran Bukhari1, Asma Rashid khan3, Nadeem Sheikh2*
Variations in proteins and transaminases following experimental induction of Bisphenol A in mice
... (12.5%) was injected in mice for
consecutive 15 days while control animals were not exposed to any treatment (n=3). Serum samples were processed
for the estimation of total protein and albumin and aminotransaminases activity. The sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) was also run to analyze protein bands. Statistically significant
increase in total protein contents, albumin and aminotransaminases wer...
consecutive 15 days while control animals were not exposed to any treatment (n=3). Serum samples were processed
for the estimation of total protein and albumin and aminotransaminases activity. The sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) was also run to analyze protein bands. Statistically significant
increase in total protein contents, albumin and aminotransaminases wer...
Madeeha Arshad, Asmatullah, Chaman Ara
Embryotoxic effects of sodium arsenate in Mus musculus
...arsenate given to gravid mice (Mus musculus). Different doses of sodium arsenate
18.25, 37.5 and 75mg/kg B.W. were administered orally at day 6 of gestation
(single exposure group) to 15 females/dose group along with one control group
having 15 females, which was given only distilled water. Pups were recovered on
18th day of gestation and fixed for morphometric morphological and histological
analysis. Morphometric observation...
18.25, 37.5 and 75mg/kg B.W. were administered orally at day 6 of gestation
(single exposure group) to 15 females/dose group along with one control group
having 15 females, which was given only distilled water. Pups were recovered on
18th day of gestation and fixed for morphometric morphological and histological
analysis. Morphometric observation...
Tafail Akbar Mughal1, Muhammad Zubair Saleem2, Shaukat Ali3,*, Khawaja Khurshid Anwar1, Muhammad Majid Bashir1, Muhammad Babar4 and Muhammad Adeeb Khan1
...) on the liver of Balb C mice and also to investigate the protective effect of Vitamin E pre-treatment on the carbon tetrachloride-induced hepatotoxicity. Study included the estimation of the activities of the enzymes such as ALAT (alanine aminotransferase), ASAT (aspartate aminotransferase) and LDH (lactate dehydrogenase) and biochemical components like glucose, urea, lipids, cholesterol and protein contents both in the liver and blood while DNA and RNA conte...
Tian-Yi Zhao1, Zi-Qing Liu2, Bo Yang1, Qiao Gao1, Hong-Yu Zhang1, Pei-Yu He1, Ming-Hua Duan1* and Yu-Li Yan1*
... blood and the spleen of mice, which was restored significantly in the number of CD71/Ter119-labeled erythrocytes and CD4/CD8-labeled T cells in 5-Fu+APs group (P < 0.001). Compared with the control group, 5-Fu group showed significantly downregulated Cyclin D1 (P < 0.001), Stat5 (P < 0.01), and Bcl-2 (P < 0.05) expression in peripheral blood cells, and it was restored in 5-Fu+APs group (P < 0.05).APs promoted ...
De-Yong Zhang1, Xiao-Lu Xu1,*, Qin Ruan2, Xiu-Ying Shen3 and Yin Lu1
...e toxicity of PFOS, male mice were administrated with a serial dosage of PFOS for 90 days for testicular observation and spermatogenesis evaluation. PFOS ≥ 11 mg/kg (accumulative dosage) resulted in visible histopathological changing in testis, including disorder and vacuolization of spermatogenic cells. Changed testicular organ coefficient (for dosage ≥ 55 mg/kg), decreased sperm concentration (for 110 mg/kg dosage), decreased motility (for 110 mg/kg do...
Abd El-Nasser Ahmed Mohammed1,2,*
...ativa) oil to female mice and secondly to explore if dietary N. sativa is effective in alleviation of hypothermia and hyperglycemia due to general anesthesia. Seventy-five albino female mice (21.74 ± 0.19) were distributed into three groups; control (G1; N=25; not receive N. sativa oil) and two N. sativa oil groups supplemented with either 1.0% (G2; N=25) or 2.0% (G3; N=25) for 30 days. Change...
Ahmad Ali1,2,* and Aleem Ahmed Khan1
...senegalensis) and mice (Mus musculus). While the percentage use of quill, mice, dove, sparrow and myna has been calculated as 66.67%, 46.24%, 46.24%, 34.41% and 21.51%, respectively. Further it was observed that falcon trapping was a teamwork and each team used multiple techniques at the same time during capturing in the field.
...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...enesis in mammals. Forty mice, randomly categorized into four groups (N=10) were administered orally with different concentrations of levofloxacin (0.00, 9.37, 18.75 and 37.50µg/g BW of mice) for 30 days consecutively. Mice were sacrificed on 31st day (24 hours after last dose administered) under deep chloroform anaesthesia. Blood was extracted through cardiac puncture and...
Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
...ects of Butyl paraben in mice and to explore the possible curative and protective impacts of turmeric. Fifty mice randomly divided in five groups, i.e. Control, dose group (BP), dose and antidote group (BP+AD), vehicle control group (VC) and antidote group (AD). A sub lethal concentration containing 15ug/g B.W of BP was given with and without turmeric orally, dissolved in corn oil, used as vehicle too. Dissections were made ...
Dilara Abbas Bukhari*, Mehwish Faheem, Shamoona Arshad and Khalid P. Lone
...hee on renal function of mice. Two locally manufactured brand of Banaspati ghee daily to mice at a rate of 10% of their daily food intake for a period of 4 weeks. After 4 weeks, both male and female mice were sacrificed and kidneys were taken out. Biochemical parameters e.g., lipid peroxidation (LPO), catalase (CAT), glutathione-S-transferase (GST), reduced glutathione (GSH) were determine...
Gasem Mohammad Abu-Taweel
... of mercuric chloride on mice offsprings’ social behavior, neuromuscular coordination, motor activity, blood parameters and liver structure alterations. On the first day of pregnancy, 30 pregnant mice were equally divided into three groups. Tap water was provided to the control group (Group I). Group II and Group III animals were orally received 5 and 10 ppm of HgCl2.The treatment started the first day of pr...
Hong-mei Gao, Wen-long Su, Gui-bin Tao, Han-yang Li, Wen-zheng Cao and Zhi-dong Qiu*
...ouracil, 5-FU) treatment mice. The mechanism of action of genistein on erythrocyte production is not clear. This study suggests that Geniposide promotes red blood cell production. We demonstrated that genistein did not affect the erythrocyte differentiation of mouse CD34+ cells. Then, we tested the geniposide indirectly affecting BM accessory cells. We conclude that geniposide maybe will control the erythropoiesis in vivo and reverse the BM m...
Mingsan Miao*, Hui Zhao, Jiaojiao Jia, Xiaoyan Fang and Yanyan Miao
...ral ischemia-reperfusion mice model and to assess the protection mechanism of cerebral ischemia-reperfusion injury. The repeated cerebral ischemia-reperfusion mice model was used where bilateral common carotid artery was targeted to block blood flow, reperfusion and then re-blocking the arteries. Brain tissues were examined 24 hours post-drug administration to determine the level of biochemical indicators such as serum NSE i...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
...ocytes of young and aged mice. Polar body extrusion was significantly delayed in aged mice (P < 0.05), however the rate of polar body extrusion was similar to that of young mice at 16 h of IVM. Moreover, PN formation of parthenogenetic embryo was significantly delayed in aged mice (P < 0.05). Afterward the two groups obtained similar results with r...
Jing Zhang1,2, Xin Wang2, Yun Zhao2, Yongcheng Jin2, Yongfeng Zhou2, Junmei Wang2, Yurong Fu2, Rui Wang2, Ruihua Li2, Hengtong Fang2 and Hao Yu2,*
...ogy of colonic mucosa in mice was normal after ZEA was administered by gavage for one week, the mRNA expression levels of mucosal Mucin-1, Mucin-2, regenerating islet-derived protein 3 gamma (Reg3γ), and tumor necrosis factor (TNF) were significantly downregulated. The mRNA expression levels of mucosal β-defensin, regenerating islet-derived protein 3 alpha (Reg3a), regenerating islet-derived protein 3 beta (Reg3β) and secretory immunoglobulin A...
Masroor Ellahi Babar*
.... It stabilizes the milk micelle and gene 5’ flanking region which works valuably in transcription regulation. Current study illustrates that Kappa-casein gene 5︠ flanking region in Dromedary camel of Pakistan is highly polymorphic and it is phylogeneticaly linked with other mammals. The analyses of the sequence of 5︠ flanking region in various breeds reveals the presence of different polymorphic regions includes cysteine nucleotide deletion, AT base...
Aamir Mushtaq1,2, Rukhsana Anwar1 and Mobasher Ahmad1,2,*
...es for which male albino mice were grouped into seven groups. Mice were subjected to elevated plus maze, hole board and light dark paradigm on (day 7th and 8th), for behavioral studies after which their brain were removed by decapitation for biochemical assessment. Results indicated the existence of total phenols (170.14 ± 1.16 mg of GAE/g of extract), flavonoids (87.67 ± 0.67 mg of RE/g o...
Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*
...rs were used to immunize mice to collect tick antiserum for WB. Then, 2-DE followed by WB and MALDI-TOF was carried out to screen and identify tick antigens. We identified 19 protein spots representing 12 ORFs: 4 from ticks and 8 from cattle. Among the the 4 ORFs, Tropomyosin, Elongation factor 1-alpha (EF-1α) and Protein Disulfide Isomerase-2 (PDI-2) have been reported as antigens and even vaccine candidate proteins in ticks or other pathogens but not i...
Yuhong Li1, Dongxue Wu1, Xue Wang1, Mi Zhang1, Hanyu Zhu1, Zhe Shi1 and Fumin Feng1,2*
...ti-tuberculosis drugs in mice. A total of 168 Kunming mice were randomly divided into four experimental groups and one baseline group. The drug groups were orally administered with isoniazid (H), rifampicin (R), pyrazinamide (Z), and combination of these three drugs (HRZ) at 3, 5, 7, 10, and 15 days, respectively. Liver tissue pathologies and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities chan...
Sara Zafar* and Asmatullah
...administered to pregnant mice by gavage. The high dose contains Atenolol 2.5 µg/g body weight (B.wt.) of treated mice and low dose contains 1.65 µg/g B.wt. The dose was administered from the 6th -12th day of the gestation period for seven days. Many congenital abnormalities including hemorrhages, hygromas, resorbed fetuses, deformed, hyperextended and hyperactive flexed limbs, distorted axis, and intrauterine gro...
Asmat Ullah1,2, Shahid Iqbal1,2 and Furhan Iqbal2*
...ective aspects of albino mice behavior in a gender specific manner. Seven week old female and male albino mice were used as experimental animals. Mice were orally supplemented either with 300 mg/ml solvent/Kg of body weight Bauhinia variegata’s leaf extract or with saline solution (0.9% NaCl) for 17 days. Behavioral observations were made by applying a series of neurological t...
Tafail Akbar Mughal1, Shaukat Ali1*, Hafiz Muhammad Tahir1, Shumaila Mumtaz1, Samaira Mumtaz1, Ali Hassan1 and Syed Akif Raza Kazmi2
...profiles of Swiss albino mice in which diabetic phenotype was induced by alloxan. A single injection of Alloxan (150 mg/kg) was applied intravenously to induce diabetic phenotype. Alloxan induction raised the overall level of triglycerides, low density- lipids (LDLs), and cholesterol and reduced the level of high density lipids (HDLs). Oral administration of 200 mg/kg aqueous extract of B. lycium Royleroot bark for 28 days rescued all these changes. Thi...
Yuan Xing, Congming Tan, Yan Luo and Wenjun Liu*
...n airway inflammation in mice and its possible mechanism of action. The mice received varying doses of quercetin from 22-30 days (1, 10 and 50 mg / kg, p.o.) and montelukast (10 mg / kg, p.o.). Intranasal OVA has been instilled on the 21 days. Biochemical parameters, spleen weight, physiological parameters, interleukin (IL-1β and IL-6) parameters and immunoglobin-E (IgE) were calculated at the end of the experimental st...
Aneeqa Saleem1, Muhammad Islam1, Hamid Saeed1* and Mehwish Iqtedar2
...>Cucumis melo L., in mice model of nephrolithiasis. Extractives of peel and pulp were obtained using hot and cold methods. Ethylene glycol (0.75% V/V) was used IP for 10 days to develop mice model of nephrolithiasis. Extracts were administered at a dose of 400 µg/g for 11 – 21 days. Outcome measures included serum creatinine, uric acid and BUN levels. Significant differences exist with regards to primary and ...
Chaman Ara1*, Asmatullah1, Saima Hanif1, Shagufta Andleeb2, Beenish Zahid3 and Madeeha Arshad4
... toxicity in male albino mice, Mus musculus. Eighty, 8-9 week old mice were divided randomly into eight groups, each containing 10 mice and exposed to three sub-lethal concentrations (37.5µg/g; 18.7µg/g; 9.37µg/g B.W) of aluminum, with and without onion extract. Control and antidote groups were treated with distilled water and onion equous extract, respectively. Do...
Samra Aftab1, Saleem Ullah1 and Farida Anjum2*
...that cellulose (49 %), hemicelluloses (23.5 %) and ash (1.8 %) were higher in sugarcane bagasse, while lignin (7.9 %) was higher in saw dust. The data regarding microbe isolation showed highest colonies of Aspergillus sp. (8 CFU × 104g-1) followed by Penicillium sp. and Trichoderma sp. (7 CFU × 104g-1 and 5 CFU × 104g-1), while the lowest colonies were noted for Candida ablican and Saccharomyces cerevisiae. The screening test of these microbe...
Xiangzu Wang,1,2 Jifeng Xi,1,2 Bin Jia,1,* Yongsheng Zhang,1 Chaocheng Li1
...2, and BAX expression in mice kidney tissue on 1 d, 7 d, 14 d, 21 d, 42 d, and 90 d after birth. The results showed that with the maturation of mouse kidney, the cell proliferation activity decreased, and the expression of ZFX, Bcl-2, and BAX were down regulated. The ZFX gene may participate in the developmental process of the kidney through the balance of cell proliferation and apoptosis regulation.
... 
Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3
...racerebrally in suckling mice then successfully identified by indirect immunofluorescence technique in the brain sections of positively inoculated mice. Reverse transcriptase polymerases chain (RT-PCR) reaction was performed directly on buffy coat samples for molecular identification of the virus showed 470 bp clear band in gel electrophoresis. The sensitivity of the utilized techniques in the identification and diagnosis of...
Sajida Batool1, Saira Batool1, Sitara Shameem1, Fatima Khalid1, Tahira Batool2, Summera Yasmeen1, Saima Batool3*
...ver of albino laboratory mice, 20 adult male mice were equally distributed (n=10) into two groups. Atrazine was given at the dose of 200 mg/kg body weight through gavages for 28 days while the control (Cnt) group remains untreated. On the 29th day, all animals were sacrificed to recover liver for histological preparations. Atrazine (ATZ) caused various hepatic implications in the treated animals. ATZ exposure caused a signif...
Kai Jin1,2,3, Chen Chen 1,2,3, Xinyu Sun1,2,3, Caiye Zhu1,2,3, Mahmoud F. Ahmed1,2,3,4, Qisheng Zuo1,2,3, Jiuzhou Song4 and Bichun Li1,2,3*
...ssion in transgenic SCD1 mice and goats. Our results suggested the possibility of generating transgenic goats by sperm-mediated gene transfer (SMGT). The expression of SCD1 gene and fatty acid metabolism genes in goat mammary gland tissues and muscular tissues was aligned with GEO database, and tested by qRT-PCR. F1 transgenic mice were mated and then the target genes and protein expression level in F2...
Chao-Qun Shi1, Hong-Ying Lin1, Jin-Qing Zheng1, Fan Yang1, Kwame Ayisi Lartey1, Dan-Ju Kang1, Hwa-Chian Robert Wang2, Ravi Gooneratne3*and Jin-Jun Chen1*
...induced diarrhoea in mice. MBC and MIC values of 0.25 and 0.125 g TFCO/ml showed it was effective as an antibacterial agent against E. coli (CMCC 44752) in vitro. Ninety Kunming mice were randomly divided into five groups of 18 mice per group (1 male: 1 female). The groups were control (saline), negative control (E. coli + 0 mg/kg TFCO), low dose (E. coli + ...
Reena Ambreen1, Sadaf Sarfraz1*, Fouzia Qamar2 and Shomaila Skinadar1
...into structures named as micelles. The formation of these micelles is accountable for the hydrophobic substances that are solubilized in surfactant-water system. The present review focuses to study the effect of various parameters like pH, temperature, additive effect, surfactant structure and its concentration on the solubilizing power of surfactant molecules resulting in the exclusion of organic coloring agents/dyes which ...
Liyun Chang1, Yingbin Chen1, Qian Kang1, JianhuaQin1,* and Zhiyong Liu2,*
...as established in Balb/c mice, followed by extraction of total RNA from small intestine of infected mice at 1, 3, 7 and 14 dpi, respectively. The relative expression of IFN-γ, TNF-α, IL-2, IL-4 and IL-6 in the small intestine of Balb/cmice infected with C. parvum, were then detected using Ct value comparison method (RT-QPCR2-ΔΔCt). The primers were...
Zheng Yan1,2,*, Shuang Liu2, Ai-Qing Liu2 and Hai-Yan Wang2
...toaged skin, BALB/C Nude mice were exposed to UVA+UVB for 16 weeks to establish the photoaging model. The concentrations of elastin peptide given to the low, medium, and high dose groups were 1.5, 5.0 and 10 mg/animal per day, respectively. Then, skin elasticity was measured using a cutimeter dual MPA 580. The concentraions of three type of collagens, hyaluronic acid and hydroxyproline in skin tissue were also determined. The results indicated that the oral ad...
Ou-Mei Hao1*, Xue-Feng Wang1, Zhi-Jun Yue1, Chun-Hong Nan1,Yan Yu1,Tong Zhang2,Yu-Feng Liu3 and Xue Zhao1
... and AZM on experimental mice. Fifty female BALB/c mice were randomly divided into five groups: control, model, AZM (0.01 g/kg/day), QZZKF (10.16 g/kg/day), AZM (0.01 g/kg/day) combined with QZZKF (10.16 g/kg/day). QZZKF and AZM were administered by oral gavage every day for five days.Pathological changes in lung tissue were determined by hematoxylin and eosin. The levels of cytokines in lung tissues were assessed by Immunoh...
Syed Shahid Imran Bukhari1*, Nusrat Jahan2, Muhammad Khalil Ahmed Khan3, Mariam Zaheer1, Sabir Javed1
... diabetic induced albino mice. Male albino mice, weighing (25±5g) were divided into three groups; Group 1: normal (non diabetic nontreated), Group 2: diabetic non treated (DNT), Group 3: diabetic treated (DT) mice. Group 3 was treated with three doses of A. barbadensis (200 mg/kg, 300 mg/kg and 400 mg/kg) per day for 14 days were assigned as DT1, DT2 and DT3 respectively. Aloe vera ...
Syukriah Syukriah1, Ulinnuha Nur Faizah2, Hendry T.S. Saragih3*, Rizki Fitrawan Yuneldi4, Soenarwan Hery Poerwanto5, Raden Roro Upiek Ngesti Wibawaning Astuti5, Stephan Immenschuh6
...n pregnant and offspring mice in Plasmodium berghei infection. A Complete randomized design was used in this study, in which 25 mice were divided into five groups. Group 1 as a control group, consisted of non-pregnant and Plasmodium-uninfected mice, group 2 comprised pregnant and uninfected mice, group 3 to 5 were pregnant mice
Fujun Miao1, Chunlan Shan2, Wei Yang3, Hao Wang3, Shuxiang Geng1 and Delu Ning1*
...olic liver disease (ALD) mice were studied. The results showed that serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the ethanol + walnut oil (ETH + WO) and ETH + silymarin positive group (PC) groups significantly decreased, and the lesions of ethanol-induced liver injury were relieved compared with the ethanol (ETH) group. Walnut oil significantly increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GS...
Hong Yin*, Dan Yang, Ji-Jie Liu, Jing-Wei Ding and Dan-Dan Cui
...was investigated in aged mice. Mice were treated without or with different doses of β-CM-7 for 30 days. At the end of the experiment, all mice were collected for serum and tissue samples. Histopathological studies showed the tissue protective role of β-CM-7 in aged mice. The treatment of β-CM-7 in aged mice<...
Doaa Sh. Mohamed1, Nema S. Shaban2 , Mai M. Labib3, Olfat Shehata4
...d oil could protect male mice against doxorubicin-induced cardiotoxicity. The experimental mice were divided into three groups; control group: received 0.9 percent saline, doxorubicin group: Mice were intraperitoneal injected with doxorubicin (5 mg/kg) ithree times over a period of two weeks (dose every five days) and almond oil group: Mice were administ...
Ilvir Khabibullin1*, Ruzel Khabibullin1, Irina Mironova1, Lyalya Musina2, Elmira Akhmadullina1, Victoria Morozova1
...e experiment showed that mice and hamsters had approximately the same indicators of physical endurance, by the end of the experiment, the swimming time of the experimental group animals was significantly longer. The animals receiving pantocrine turned to be stronger. Their interval time increased by 267 seconds (Р≤0.05) while the swim time of animals consuming may chang rose by 96 seconds (Р≤0.05) compared to the control. Male hamsters consuming lemong...
Sana Shakoor, Tahir Rehman Samiullah, Naila Shahid, Abdul Qayyum Rao*, Aneela Yasmeen, Sana Tahir, Ayesha Latif, Saira Azam, Ahmad Ali Shahid and Tayyab Husnain
...ritoneal immunization of mice with HA protein along with enhancer (ferund adjuvants) was done and produced antibodies in serum was detected by immunodot blotting assay. The appearance of the dot on spotted position confirmed the specificity of anti-HA antibodies. The titer of antibody produced in immunogenic mice was determined through ELISA. The highest geometric mean titer of HA-specific serum IgG, which was greater as com...
Ishrat Younus1,2 and Afshan Siddiq1*
... var. caudatus in albino mice. The analgesic and antipyretic activities were determined at three different doses (50, 100 and 200 mg/kg) using different pain models (writhing induced, tail flick) and yeast induced pyrexia tests, respectively. The extract exhibited significant (p < 0.05) in vivo analgesic and antipyretic potential in term of % writhes inhibition, increased latency time and reduced rectal temperature when compared with control. The extract di...
Dwi Budiono1,5, Ratih Rinendyaputri2, Ariyani Noviantari2, Mokhamad Fahrudin3, Ni Luh Putu Ika Mayasari4, Vista Budiariati1,6, Diah Nugrahani Pristihadi1,3, Noer Muhamad Dliyaul Haq1, Arief Boediono3*
... effective in culture of mice parthenogenetic embryos. This is due to CHIR99021 is able to increase blastocyst rate, blastocyst quality, and formation of pESC colonies with improved quality.
Keywords | Blastocyst, CHIR99021, Embryonic stem cell, Parthenogenetic, Primary colony
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