Felwa A. Thagfan1, Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1
.../i> induced infection in mice jejunum. Three different doses, 300, 600 and 900 mg/kg were used after infection of mice with sporulated oocysts. A dose of 300 mg/kg reduced the number of oocyst output in mice faeces by about 56.8 % and also improved the body weight. In addition, the root extract decreased the number of parasitic stages and the number of goblet cells in the jejunal villi in ...
Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*
... total of 30 female BWF1 mice were randomly divided into three groups as follows: group (I) control group (lupus uninfected); group (II) live P. chabaudi infected group (lupus + live P. chabaudi infection); and group (III) irradiated P. chabaudi-infected group (lupus + irradiated P. chabaudi-infection). All groups were killed at day 14 post infection. Histological and biochemical investigations were performed.Live P. chabaudi infection was accompanied with an ...
Faiqah Ramzan*1 and Muhammad Haris Ramzan2
...tric acid levels in male mice. Kisspeptin-10 was administered intraperitoneally in different dosage concentrations (1μg, 1 ng, and 10 ρg) to adult male mice twice daily for 12 days. Prostatic citric acid levels were determined photometrically. The levels decreased significantly at all tested doses. Intermittent administration of Kisspeptin –10 negatively regulates the prostatic citric acid levels in adult male <...
Jixue Hou1,2, Xueling Chen3, Jing Wang4, Hongwei Zhang2, Yu Xi2, Jie Xia2, Xinyu Peng2,* and Xiangwei Wu2,5*
...bbits, and big rats. The mice with reproduction fast cycle, lower costing, and close to human genes, can be showed about some phenomenon quickly, also more easy to form model of atherosclerosis. Thus, a mouse model of vascular injury is vitally important for researching the pathophysiological mechanisms to restenosis after percutaneous transluminal coronary angioplasty (PTCA) as well as translational approaches. In this Experiment, we established a unique muri...
Tianzhu Chao, Huiqiang Cai, Yuxun Zhou, Kai Li* and Junhua Xiao
...enetics of Chinese house mice based on 466 DNA sequences from mitochondrial D-loop fragments. Our analyses revealed that Mus musculus musculus and M. m. castaneus are older colonized subspecies with broad distributions in East China, while M. m. domestics is a newly arrived subspecies currently restricted to Shanghai, indicating the elevated risk in this cosmopolitan cities. Phylogenetic analysis indicates that M. m. musculus migrat...
Yi-qiang Ouyang1, Zhen-xin Liang1, Shi-wen Huang2, Ying Zhang3, Shao-shi Luo1, Jin-ning Liang1, Jia-fu Li1, Yu He4 and Song-chao Guo1,*
...se (AD) involve rats and mice, yet the tree shrew (Tupaia belangeri) is in many respects more similar to primates than rodents are. To demonstrate the feasibility and usefulness of using the tree shrew as an AD model, we examined how well the model recapitulates features of the disease. Injecting animals intraperitoneally with d-galactose to induce acute senility, and then injecting them with β amyloid fragment 1-42 and ibotenic acid into the bilat...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
...njected in female BALB/c mice to induce the disease. After five months, mice in various groups were treated with prednisone and fed with gluten containing and standard diet for four weeks and applied procedure to detect minor changes in paw swelling, ANA autoantibodies, CCL11, C3c, glucose level and renal damage. We detected increased symptoms of arthritis and gastrointestinal tract involvement in gluten containing diet grou...
Madeeha Arshad1, Naveed Ahmad2,*, Muhammad Khalid3, Asmatullah1, Mohammad Tahir2, Khadija Naveed1 and Asia Iqbal4
...administered to pregnant mice. LD50 of Cr VI, was calculated as 88mg/kg of B.W in pregnant mice. Among 05 groups of pregnant females (10 females in each group), three were treated with 50%, 25% and 12.5% of the LD50 of Cr VI, respectively, with one control and one vehicle control group. Fetuses were recovered on 18th day of gestation and fixed for morphometric and morphological studies. Exper...
Fariha Imtiaz1, Muhammad Islam1, Hamid Saeed2,*, Bushra Saleem1, Maryam Asghar1 and Zikria Saleem2
...xtracts and minoxidil on mice hair diameter. Taken together, our data suggested that ethanol extracts of leaves of T. foenum-graecum had significantly higher growth promoting effects compared to standard, i.e., minoxidil. Thus, results from our study set the stage of further studies to identify the constituents in ethanol extracts, responsible for hair growth promoting effects.
...
Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
...vival in macrophages and mice than the parent strain 2308. With decrease in the spleen/body weight ratios of mice infected with omp10 mutants were noted, inhibition of phagosome-Lysosome fusion was dramaticly impaired, and profound increase of apoptosis rate after exposure of RAW264.7 to omp10 mutants at 12 h, 24 h and 48 h compared to RAW264.7 exposed to 2308. Results indicate that Δomp10 mutant w...
Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3*
...in xenotransplanted SCID mice and by histopathological and immunohistochemical analyses. Our results demonstrated that fusion protein VEGFR1(D1-D3)-Fc efficiently inhibited
the lung cancer growth in vivo. Although the aptamer RNV66 was not found to be as efficient in this study, the results were also not surprising as the aptamer was administered naked and at low dose. As these are only our preliminary studies, a detailed investigation is planned using ...
the lung cancer growth in vivo. Although the aptamer RNV66 was not found to be as efficient in this study, the results were also not surprising as the aptamer was administered naked and at low dose. As these are only our preliminary studies, a detailed investigation is planned using ...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2
...inistered to male albino mice for up to eight weeks for growth promoting activity. Increased mammary growth was observed in test female non lactating mice, as compared to controls when placental lactogen was applied. Increase weight gain for visceral tissues like kidney, liver, muscle and lungs was also observed, as compared to control subjects. It was also recorded that the exogenous supply of bubaline PL reduces fat cells ...
Rabia Qurashi, Qurat ul Ane Gillani* and Furhan Iqbal*
...f 6 week old male albino mice. Blood samples from male albino mice (CGP55845 treated N = 14 and saline treated N = 16) were collected directly by cardiac puncture and used for estimation of hematological and some serum biochemical parameters. The hematological parameters did not show any significant variation between the two groups. From amongst serum biochemical parameters triglycerides (P = 0.02) in CGP55845 treated
Abd El-Nasser Ahmed Mohammed*
... upon transplantation in mice and rats. Eighteen adult female rats were classified to three equal groups in addition to sex young females as donor of young ovaries. Similar classification was done using eighteen female mice and sex young females. The females of mice or rats were anesthetized for ovarian transplantation using (6.2 and 13.2 mg/kg BW) and (13.3 and 26.6 mg/kg BW) diazepam and...
Tania A. Shakoori1,*, Soumble Zulfiqar2, Khadija Irfan Khawaja3 and Faisal Masud4
...R in adipose tissue from mice treated with NaB versus placebo. Four groups of mice (n=27) mice were administered oral NaB, intraperitoneal (i.p) NaB, oral placebo or i.p placebo for 16 days. RNA was isolated from the epididymal fat pads and relative genetic expression of AMPK, Sirt1, mTOR and Akt was measured using real time PCR. Oral NaB group showed decreased genetic expression of AMPK (...
Asmatullah1, Chaman Ara1,*, Shagufta Andleeb2, Maha Tahir1, Beenish Zahid1 and Madeeha Arshad1
...by cadmium in developing mice. For this purpose, different aqueous concentrations of cadmium chloride i.e. 0.00, 6.25, 12.50, 25.00 µg/g B.W of mice were given orally to pregnant mice on days 6-12 of gestation daily. Another series of mice were treated with different concentrations of cadmium chloride and also with an antidote (guava fruit e...
Basit Zeshan1,2,*, Mushtaq A. Saleem2,Javed Iqbal Wattoo2, Mohd Mokhtar Arshad1 and Maizan Mohamed1
... an animal experiment on mice, in which mice were injected subcutaneously. These findings suggest that the truncated Sf200 expressed in the pET-32a (+) prokaryotic vector can be used as antigen to detect antibodies against IBV.
...
Kaori Sasaoka1, Takahiro Kataoka1, Norie Kanzaki1, Yusuke Kobashi1, Akihiro Sakoda2, Yuu Ishimori2 and Kiyonori Yamaoka1,*
...or highly radiosensitive mice. CDDP was administered at 20 mg/kg weight to C57BL/6J and BALB/c mice after radon inhalation at 1000 Bq/m3 and 2000 Bq/m3 for 24 h. Radon inhalation had a slight positive effect against CDDP toxicity in C57BL/6J mice with respect to improved hair condition, whereas radon inhalation exacerbated CDDP-induced toxicity in BALB/c
Muhammad Nazar Aftab1, Ahmed Ali1, Muhammad Asad1, Sadia Fatima2 and Furhan Iqbal2,*
...l profile of male albino mice. Eight week old male albino mice were used as experimental animals and were divided into two groups. First group was treated with 80mg/Kg body weight of AlCl3 for 16 days while control group was treated with saline solution for the same period of time. Complete blood count were determined in both experimental treatments at the end of dose supplementation. Our results revealed that ora...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...sequently, thirty female mice randomly were divided into three groups, treating with 50 μg pEGS/2SS-tPA (group T1), 50 μg pEGS/2SS (group T2), and 50 μg pEGFP-N1 (Control group) at weeks 0, 2, 4 and 6. Mating with male mice was performed at 2 weeks post final immunization. Results showed that mice both in T1 and T2 groups elicited significantly stronger anti-SS antibody level than...
Madeeha Arshad1, Asmatullah1, Chaman Ara1, Shagufta Andleeb2 and Naveed Ahmad3,*
...developmental defects in mice fetuses. Different concentrations of the drug, 0.00, 4.10, 8.33 and 16.66 µg/g B.W. were administered orally to the dams on days 6-12 of gestation, and fetuses were recovered on day 18. Morphological observations of fetuses from 4.10, 8.33 and 16.66 µg/g B.W. dose groups showed abnormalities such as skin hemorrhages, microphthalmia, limb deformities, hygromas, kyphosis, curved and short tail and fluid filled abdominal ...
Zain ul Abidin1, Aisha Khatoon2, Abdul Whab Manzoor1,*, Nida Arooj1, Sajjad Ali1 and Muhammad Numan1
...legs were noticed in the mice of both groups between 11-15 days post inoculation while all the mice were found dead between 15-18 days post inoculation in mouse inoculation test (MIT). Reverse transcriptase-polymerase chain reaction (RT-PCR) performed for both samples gave a product of 443-bp amplifying the highly conserved “N”-region gene of virus confirming the rabies infection in both cases.
...
Imran Taj1,*, Ferhat Abbas1, Zafar Ahmad1, Mohammad Kamran Taj1, Deedar Ahmad2, Zahoor Ahmed2, Ghulam Mohammad2, Ajaz-ul-Haq1, Farooq Shahzad1 and Sabiha Azam3
...reas and gall bladder of mice.
... 
Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2
Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
...c anomalies. Male albino mice (Mus musculus) were equally divided (n=10) as C; control, Cr-treatedandCr-JgroupsreceivingCr+6 in the form of potassium dichromate (K2Cr2O7) 50ppm for 10days ad-libitum while Cr-J group additionally given 0.25ml/12h Jambul Fruit Extract (JFE) for next 5days by oral gavage. On the 16th day blood, liver and kidney were collected for biochemical and histopathological analysis.Cr+6 treated group showed severe histological changes like...
Muhammad Mohsin Ahsan1, Hafiz Muhammad Tahir2, Muhammad Khalid Mukhtar1
Effect of lunar cycle on active population density of scorpions, their potential prey and predators
...ws, lizards, centipedes, mice and rats) was noted in the presence or absence of moonlight, but they were
observed to be more active during the moonlight period than during moonless nights....
observed to be more active during the moonlight period than during moonless nights....
Muddasir Hassan Abbasi1, 2, Noor Fatima1, Syed Shahid Imran Bukhari1, Asma Rashid khan3, Nadeem Sheikh2*
Variations in proteins and transaminases following experimental induction of Bisphenol A in mice
... (12.5%) was injected in mice for
consecutive 15 days while control animals were not exposed to any treatment (n=3). Serum samples were processed
for the estimation of total protein and albumin and aminotransaminases activity. The sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) was also run to analyze protein bands. Statistically significant
increase in total protein contents, albumin and aminotransaminases wer...
consecutive 15 days while control animals were not exposed to any treatment (n=3). Serum samples were processed
for the estimation of total protein and albumin and aminotransaminases activity. The sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) was also run to analyze protein bands. Statistically significant
increase in total protein contents, albumin and aminotransaminases wer...
Madeeha Arshad, Asmatullah, Chaman Ara
Embryotoxic effects of sodium arsenate in Mus musculus
...arsenate given to gravid mice (Mus musculus). Different doses of sodium arsenate
18.25, 37.5 and 75mg/kg B.W. were administered orally at day 6 of gestation
(single exposure group) to 15 females/dose group along with one control group
having 15 females, which was given only distilled water. Pups were recovered on
18th day of gestation and fixed for morphometric morphological and histological
analysis. Morphometric observation...
18.25, 37.5 and 75mg/kg B.W. were administered orally at day 6 of gestation
(single exposure group) to 15 females/dose group along with one control group
having 15 females, which was given only distilled water. Pups were recovered on
18th day of gestation and fixed for morphometric morphological and histological
analysis. Morphometric observation...
Tafail Akbar Mughal1, Muhammad Zubair Saleem2, Shaukat Ali3,*, Khawaja Khurshid Anwar1, Muhammad Majid Bashir1, Muhammad Babar4 and Muhammad Adeeb Khan1
...) on the liver of Balb C mice and also to investigate the protective effect of Vitamin E pre-treatment on the carbon tetrachloride-induced hepatotoxicity. Study included the estimation of the activities of the enzymes such as ALAT (alanine aminotransferase), ASAT (aspartate aminotransferase) and LDH (lactate dehydrogenase) and biochemical components like glucose, urea, lipids, cholesterol and protein contents both in the liver and blood while DNA and RNA conte...
Tian-Yi Zhao1, Zi-Qing Liu2, Bo Yang1, Qiao Gao1, Hong-Yu Zhang1, Pei-Yu He1, Ming-Hua Duan1* and Yu-Li Yan1*
... blood and the spleen of mice, which was restored significantly in the number of CD71/Ter119-labeled erythrocytes and CD4/CD8-labeled T cells in 5-Fu+APs group (P < 0.001). Compared with the control group, 5-Fu group showed significantly downregulated Cyclin D1 (P < 0.001), Stat5 (P < 0.01), and Bcl-2 (P < 0.05) expression in peripheral blood cells, and it was restored in 5-Fu+APs group (P < 0.05).APs promoted ...
De-Yong Zhang1, Xiao-Lu Xu1,*, Qin Ruan2, Xiu-Ying Shen3 and Yin Lu1
...e toxicity of PFOS, male mice were administrated with a serial dosage of PFOS for 90 days for testicular observation and spermatogenesis evaluation. PFOS ≥ 11 mg/kg (accumulative dosage) resulted in visible histopathological changing in testis, including disorder and vacuolization of spermatogenic cells. Changed testicular organ coefficient (for dosage ≥ 55 mg/kg), decreased sperm concentration (for 110 mg/kg dosage), decreased motility (for 110 mg/kg do...
Abd El-Nasser Ahmed Mohammed1,2,*
...ativa) oil to female mice and secondly to explore if dietary N. sativa is effective in alleviation of hypothermia and hyperglycemia due to general anesthesia. Seventy-five albino female mice (21.74 ± 0.19) were distributed into three groups; control (G1; N=25; not receive N. sativa oil) and two N. sativa oil groups supplemented with either 1.0% (G2; N=25) or 2.0% (G3; N=25) for 30 days. Change...
Ahmad Ali1,2,* and Aleem Ahmed Khan1
...senegalensis) and mice (Mus musculus). While the percentage use of quill, mice, dove, sparrow and myna has been calculated as 66.67%, 46.24%, 46.24%, 34.41% and 21.51%, respectively. Further it was observed that falcon trapping was a teamwork and each team used multiple techniques at the same time during capturing in the field.
...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...enesis in mammals. Forty mice, randomly categorized into four groups (N=10) were administered orally with different concentrations of levofloxacin (0.00, 9.37, 18.75 and 37.50µg/g BW of mice) for 30 days consecutively. Mice were sacrificed on 31st day (24 hours after last dose administered) under deep chloroform anaesthesia. Blood was extracted through cardiac puncture and...
Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
...ects of Butyl paraben in mice and to explore the possible curative and protective impacts of turmeric. Fifty mice randomly divided in five groups, i.e. Control, dose group (BP), dose and antidote group (BP+AD), vehicle control group (VC) and antidote group (AD). A sub lethal concentration containing 15ug/g B.W of BP was given with and without turmeric orally, dissolved in corn oil, used as vehicle too. Dissections were made ...
Dilara Abbas Bukhari*, Mehwish Faheem, Shamoona Arshad and Khalid P. Lone
...hee on renal function of mice. Two locally manufactured brand of Banaspati ghee daily to mice at a rate of 10% of their daily food intake for a period of 4 weeks. After 4 weeks, both male and female mice were sacrificed and kidneys were taken out. Biochemical parameters e.g., lipid peroxidation (LPO), catalase (CAT), glutathione-S-transferase (GST), reduced glutathione (GSH) were determine...
Gasem Mohammad Abu-Taweel
... of mercuric chloride on mice offsprings’ social behavior, neuromuscular coordination, motor activity, blood parameters and liver structure alterations. On the first day of pregnancy, 30 pregnant mice were equally divided into three groups. Tap water was provided to the control group (Group I). Group II and Group III animals were orally received 5 and 10 ppm of HgCl2.The treatment started the first day of pr...
Hong-mei Gao, Wen-long Su, Gui-bin Tao, Han-yang Li, Wen-zheng Cao and Zhi-dong Qiu*
...ouracil, 5-FU) treatment mice. The mechanism of action of genistein on erythrocyte production is not clear. This study suggests that Geniposide promotes red blood cell production. We demonstrated that genistein did not affect the erythrocyte differentiation of mouse CD34+ cells. Then, we tested the geniposide indirectly affecting BM accessory cells. We conclude that geniposide maybe will control the erythropoiesis in vivo and reverse the BM m...
Mingsan Miao*, Hui Zhao, Jiaojiao Jia, Xiaoyan Fang and Yanyan Miao
...ral ischemia-reperfusion mice model and to assess the protection mechanism of cerebral ischemia-reperfusion injury. The repeated cerebral ischemia-reperfusion mice model was used where bilateral common carotid artery was targeted to block blood flow, reperfusion and then re-blocking the arteries. Brain tissues were examined 24 hours post-drug administration to determine the level of biochemical indicators such as serum NSE i...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
...ocytes of young and aged mice. Polar body extrusion was significantly delayed in aged mice (P < 0.05), however the rate of polar body extrusion was similar to that of young mice at 16 h of IVM. Moreover, PN formation of parthenogenetic embryo was significantly delayed in aged mice (P < 0.05). Afterward the two groups obtained similar results with r...
Jing Zhang1,2, Xin Wang2, Yun Zhao2, Yongcheng Jin2, Yongfeng Zhou2, Junmei Wang2, Yurong Fu2, Rui Wang2, Ruihua Li2, Hengtong Fang2 and Hao Yu2,*
...ogy of colonic mucosa in mice was normal after ZEA was administered by gavage for one week, the mRNA expression levels of mucosal Mucin-1, Mucin-2, regenerating islet-derived protein 3 gamma (Reg3γ), and tumor necrosis factor (TNF) were significantly downregulated. The mRNA expression levels of mucosal β-defensin, regenerating islet-derived protein 3 alpha (Reg3a), regenerating islet-derived protein 3 beta (Reg3β) and secretory immunoglobulin A...
Masroor Ellahi Babar*
.... It stabilizes the milk micelle and gene 5’ flanking region which works valuably in transcription regulation. Current study illustrates that Kappa-casein gene 5︠ flanking region in Dromedary camel of Pakistan is highly polymorphic and it is phylogeneticaly linked with other mammals. The analyses of the sequence of 5︠ flanking region in various breeds reveals the presence of different polymorphic regions includes cysteine nucleotide deletion, AT base...
Aamir Mushtaq1,2, Rukhsana Anwar1 and Mobasher Ahmad1,2,*
...es for which male albino mice were grouped into seven groups. Mice were subjected to elevated plus maze, hole board and light dark paradigm on (day 7th and 8th), for behavioral studies after which their brain were removed by decapitation for biochemical assessment. Results indicated the existence of total phenols (170.14 ± 1.16 mg of GAE/g of extract), flavonoids (87.67 ± 0.67 mg of RE/g o...
Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*
...rs were used to immunize mice to collect tick antiserum for WB. Then, 2-DE followed by WB and MALDI-TOF was carried out to screen and identify tick antigens. We identified 19 protein spots representing 12 ORFs: 4 from ticks and 8 from cattle. Among the the 4 ORFs, Tropomyosin, Elongation factor 1-alpha (EF-1α) and Protein Disulfide Isomerase-2 (PDI-2) have been reported as antigens and even vaccine candidate proteins in ticks or other pathogens but not i...
Yuhong Li1, Dongxue Wu1, Xue Wang1, Mi Zhang1, Hanyu Zhu1, Zhe Shi1 and Fumin Feng1,2*
...ti-tuberculosis drugs in mice. A total of 168 Kunming mice were randomly divided into four experimental groups and one baseline group. The drug groups were orally administered with isoniazid (H), rifampicin (R), pyrazinamide (Z), and combination of these three drugs (HRZ) at 3, 5, 7, 10, and 15 days, respectively. Liver tissue pathologies and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities chan...
Sara Zafar* and Asmatullah
...administered to pregnant mice by gavage. The high dose contains Atenolol 2.5 µg/g body weight (B.wt.) of treated mice and low dose contains 1.65 µg/g B.wt. The dose was administered from the 6th -12th day of the gestation period for seven days. Many congenital abnormalities including hemorrhages, hygromas, resorbed fetuses, deformed, hyperextended and hyperactive flexed limbs, distorted axis, and intrauterine gro...
Asmat Ullah1,2, Shahid Iqbal1,2 and Furhan Iqbal2*
...ective aspects of albino mice behavior in a gender specific manner. Seven week old female and male albino mice were used as experimental animals. Mice were orally supplemented either with 300 mg/ml solvent/Kg of body weight Bauhinia variegata’s leaf extract or with saline solution (0.9% NaCl) for 17 days. Behavioral observations were made by applying a series of neurological t...
Tafail Akbar Mughal1, Shaukat Ali1*, Hafiz Muhammad Tahir1, Shumaila Mumtaz1, Samaira Mumtaz1, Ali Hassan1 and Syed Akif Raza Kazmi2
...profiles of Swiss albino mice in which diabetic phenotype was induced by alloxan. A single injection of Alloxan (150 mg/kg) was applied intravenously to induce diabetic phenotype. Alloxan induction raised the overall level of triglycerides, low density- lipids (LDLs), and cholesterol and reduced the level of high density lipids (HDLs). Oral administration of 200 mg/kg aqueous extract of B. lycium Royleroot bark for 28 days rescued all these changes. Thi...
Yuan Xing, Congming Tan, Yan Luo and Wenjun Liu*
...n airway inflammation in mice and its possible mechanism of action. The mice received varying doses of quercetin from 22-30 days (1, 10 and 50 mg / kg, p.o.) and montelukast (10 mg / kg, p.o.). Intranasal OVA has been instilled on the 21 days. Biochemical parameters, spleen weight, physiological parameters, interleukin (IL-1β and IL-6) parameters and immunoglobin-E (IgE) were calculated at the end of the experimental st...
Aneeqa Saleem1, Muhammad Islam1, Hamid Saeed1* and Mehwish Iqtedar2
...>Cucumis melo L., in mice model of nephrolithiasis. Extractives of peel and pulp were obtained using hot and cold methods. Ethylene glycol (0.75% V/V) was used IP for 10 days to develop mice model of nephrolithiasis. Extracts were administered at a dose of 400 µg/g for 11 – 21 days. Outcome measures included serum creatinine, uric acid and BUN levels. Significant differences exist with regards to primary and ...
Chaman Ara1*, Asmatullah1, Saima Hanif1, Shagufta Andleeb2, Beenish Zahid3 and Madeeha Arshad4
... toxicity in male albino mice, Mus musculus. Eighty, 8-9 week old mice were divided randomly into eight groups, each containing 10 mice and exposed to three sub-lethal concentrations (37.5µg/g; 18.7µg/g; 9.37µg/g B.W) of aluminum, with and without onion extract. Control and antidote groups were treated with distilled water and onion equous extract, respectively. Do...
Samra Aftab1, Saleem Ullah1 and Farida Anjum2*
...that cellulose (49 %), hemicelluloses (23.5 %) and ash (1.8 %) were higher in sugarcane bagasse, while lignin (7.9 %) was higher in saw dust. The data regarding microbe isolation showed highest colonies of Aspergillus sp. (8 CFU × 104g-1) followed by Penicillium sp. and Trichoderma sp. (7 CFU × 104g-1 and 5 CFU × 104g-1), while the lowest colonies were noted for Candida ablican and Saccharomyces cerevisiae. The screening test of these microbe...
Xiangzu Wang,1,2 Jifeng Xi,1,2 Bin Jia,1,* Yongsheng Zhang,1 Chaocheng Li1
...2, and BAX expression in mice kidney tissue on 1 d, 7 d, 14 d, 21 d, 42 d, and 90 d after birth. The results showed that with the maturation of mouse kidney, the cell proliferation activity decreased, and the expression of ZFX, Bcl-2, and BAX were down regulated. The ZFX gene may participate in the developmental process of the kidney through the balance of cell proliferation and apoptosis regulation.
... 
Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3
...racerebrally in suckling mice then successfully identified by indirect immunofluorescence technique in the brain sections of positively inoculated mice. Reverse transcriptase polymerases chain (RT-PCR) reaction was performed directly on buffy coat samples for molecular identification of the virus showed 470 bp clear band in gel electrophoresis. The sensitivity of the utilized techniques in the identification and diagnosis of...
Sajida Batool1, Saira Batool1, Sitara Shameem1, Fatima Khalid1, Tahira Batool2, Summera Yasmeen1, Saima Batool3*
...ver of albino laboratory mice, 20 adult male mice were equally distributed (n=10) into two groups. Atrazine was given at the dose of 200 mg/kg body weight through gavages for 28 days while the control (Cnt) group remains untreated. On the 29th day, all animals were sacrificed to recover liver for histological preparations. Atrazine (ATZ) caused various hepatic implications in the treated animals. ATZ exposure caused a signif...
Kai Jin1,2,3, Chen Chen 1,2,3, Xinyu Sun1,2,3, Caiye Zhu1,2,3, Mahmoud F. Ahmed1,2,3,4, Qisheng Zuo1,2,3, Jiuzhou Song4 and Bichun Li1,2,3*
...ssion in transgenic SCD1 mice and goats. Our results suggested the possibility of generating transgenic goats by sperm-mediated gene transfer (SMGT). The expression of SCD1 gene and fatty acid metabolism genes in goat mammary gland tissues and muscular tissues was aligned with GEO database, and tested by qRT-PCR. F1 transgenic mice were mated and then the target genes and protein expression level in F2...
Chao-Qun Shi1, Hong-Ying Lin1, Jin-Qing Zheng1, Fan Yang1, Kwame Ayisi Lartey1, Dan-Ju Kang1, Hwa-Chian Robert Wang2, Ravi Gooneratne3*and Jin-Jun Chen1*
...induced diarrhoea in mice. MBC and MIC values of 0.25 and 0.125 g TFCO/ml showed it was effective as an antibacterial agent against E. coli (CMCC 44752) in vitro. Ninety Kunming mice were randomly divided into five groups of 18 mice per group (1 male: 1 female). The groups were control (saline), negative control (E. coli + 0 mg/kg TFCO), low dose (E. coli + ...
Reena Ambreen1, Sadaf Sarfraz1*, Fouzia Qamar2 and Shomaila Skinadar1
...into structures named as micelles. The formation of these micelles is accountable for the hydrophobic substances that are solubilized in surfactant-water system. The present review focuses to study the effect of various parameters like pH, temperature, additive effect, surfactant structure and its concentration on the solubilizing power of surfactant molecules resulting in the exclusion of organic coloring agents/dyes which ...
Liyun Chang1, Yingbin Chen1, Qian Kang1, JianhuaQin1,* and Zhiyong Liu2,*
...as established in Balb/c mice, followed by extraction of total RNA from small intestine of infected mice at 1, 3, 7 and 14 dpi, respectively. The relative expression of IFN-γ, TNF-α, IL-2, IL-4 and IL-6 in the small intestine of Balb/cmice infected with C. parvum, were then detected using Ct value comparison method (RT-QPCR2-ΔΔCt). The primers were...
Zheng Yan1,2,*, Shuang Liu2, Ai-Qing Liu2 and Hai-Yan Wang2
...toaged skin, BALB/C Nude mice were exposed to UVA+UVB for 16 weeks to establish the photoaging model. The concentrations of elastin peptide given to the low, medium, and high dose groups were 1.5, 5.0 and 10 mg/animal per day, respectively. Then, skin elasticity was measured using a cutimeter dual MPA 580. The concentraions of three type of collagens, hyaluronic acid and hydroxyproline in skin tissue were also determined. The results indicated that the oral ad...
Ou-Mei Hao1*, Xue-Feng Wang1, Zhi-Jun Yue1, Chun-Hong Nan1,Yan Yu1,Tong Zhang2,Yu-Feng Liu3 and Xue Zhao1
... and AZM on experimental mice. Fifty female BALB/c mice were randomly divided into five groups: control, model, AZM (0.01 g/kg/day), QZZKF (10.16 g/kg/day), AZM (0.01 g/kg/day) combined with QZZKF (10.16 g/kg/day). QZZKF and AZM were administered by oral gavage every day for five days.Pathological changes in lung tissue were determined by hematoxylin and eosin. The levels of cytokines in lung tissues were assessed by Immunoh...
Syed Shahid Imran Bukhari1*, Nusrat Jahan2, Muhammad Khalil Ahmed Khan3, Mariam Zaheer1, Sabir Javed1
... diabetic induced albino mice. Male albino mice, weighing (25±5g) were divided into three groups; Group 1: normal (non diabetic nontreated), Group 2: diabetic non treated (DNT), Group 3: diabetic treated (DT) mice. Group 3 was treated with three doses of A. barbadensis (200 mg/kg, 300 mg/kg and 400 mg/kg) per day for 14 days were assigned as DT1, DT2 and DT3 respectively. Aloe vera ...
Syukriah Syukriah1, Ulinnuha Nur Faizah2, Hendry T.S. Saragih3*, Rizki Fitrawan Yuneldi4, Soenarwan Hery Poerwanto5, Raden Roro Upiek Ngesti Wibawaning Astuti5, Stephan Immenschuh6
...n pregnant and offspring mice in Plasmodium berghei infection. A Complete randomized design was used in this study, in which 25 mice were divided into five groups. Group 1 as a control group, consisted of non-pregnant and Plasmodium-uninfected mice, group 2 comprised pregnant and uninfected mice, group 3 to 5 were pregnant mice
Fujun Miao1, Chunlan Shan2, Wei Yang3, Hao Wang3, Shuxiang Geng1 and Delu Ning1*
...olic liver disease (ALD) mice were studied. The results showed that serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the ethanol + walnut oil (ETH + WO) and ETH + silymarin positive group (PC) groups significantly decreased, and the lesions of ethanol-induced liver injury were relieved compared with the ethanol (ETH) group. Walnut oil significantly increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GS...
Hong Yin*, Dan Yang, Ji-Jie Liu, Jing-Wei Ding and Dan-Dan Cui
...was investigated in aged mice. Mice were treated without or with different doses of β-CM-7 for 30 days. At the end of the experiment, all mice were collected for serum and tissue samples. Histopathological studies showed the tissue protective role of β-CM-7 in aged mice. The treatment of β-CM-7 in aged mice<...
Doaa Sh. Mohamed1, Nema S. Shaban2 , Mai M. Labib3, Olfat Shehata4
...d oil could protect male mice against doxorubicin-induced cardiotoxicity. The experimental mice were divided into three groups; control group: received 0.9 percent saline, doxorubicin group: Mice were intraperitoneal injected with doxorubicin (5 mg/kg) ithree times over a period of two weeks (dose every five days) and almond oil group: Mice were administ...
Ilvir Khabibullin1*, Ruzel Khabibullin1, Irina Mironova1, Lyalya Musina2, Elmira Akhmadullina1, Victoria Morozova1
...e experiment showed that mice and hamsters had approximately the same indicators of physical endurance, by the end of the experiment, the swimming time of the experimental group animals was significantly longer. The animals receiving pantocrine turned to be stronger. Their interval time increased by 267 seconds (Р≤0.05) while the swim time of animals consuming may chang rose by 96 seconds (Р≤0.05) compared to the control. Male hamsters consuming lemong...
Sana Shakoor, Tahir Rehman Samiullah, Naila Shahid, Abdul Qayyum Rao*, Aneela Yasmeen, Sana Tahir, Ayesha Latif, Saira Azam, Ahmad Ali Shahid and Tayyab Husnain
...ritoneal immunization of mice with HA protein along with enhancer (ferund adjuvants) was done and produced antibodies in serum was detected by immunodot blotting assay. The appearance of the dot on spotted position confirmed the specificity of anti-HA antibodies. The titer of antibody produced in immunogenic mice was determined through ELISA. The highest geometric mean titer of HA-specific serum IgG, which was greater as com...
Ishrat Younus1,2 and Afshan Siddiq1*
... var. caudatus in albino mice. The analgesic and antipyretic activities were determined at three different doses (50, 100 and 200 mg/kg) using different pain models (writhing induced, tail flick) and yeast induced pyrexia tests, respectively. The extract exhibited significant (p < 0.05) in vivo analgesic and antipyretic potential in term of % writhes inhibition, increased latency time and reduced rectal temperature when compared with control. The extract di...
Dwi Budiono1,5, Ratih Rinendyaputri2, Ariyani Noviantari2, Mokhamad Fahrudin3, Ni Luh Putu Ika Mayasari4, Vista Budiariati1,6, Diah Nugrahani Pristihadi1,3, Noer Muhamad Dliyaul Haq1, Arief Boediono3*
... effective in culture of mice parthenogenetic embryos. This is due to CHIR99021 is able to increase blastocyst rate, blastocyst quality, and formation of pESC colonies with improved quality.
Keywords | Blastocyst, CHIR99021, Embryonic stem cell, Parthenogenetic, Primary colony
...
De Hu, Jia Wang, Huan Wang, Xiang-Yang Leng, Tian-Yi Zhao* and Shu-Min Wang*
... on gastric ulcer, fifty mice were randomly divided into 5 groups (10 mice per group), namely control (normal saline), model (normal saline), ranitidine group (0.03 g/kg) and FP groups (1 and 2 g/kg). Stress gastric ulcer was induced by water-immersion and restraint stress (WIRS) after 10 days of drug administration. To observe the histopathological changes of gastric mucosa. the activities of pepsin, superoxide dismutase (S...
Yuechen Li1, Yumo Li1, Xuefeng Zhuang1, Guangfu Lv2, Xiaowei Huang1, Zhe Lin1, Yuchen Wang1* and He Lin1*
... OR on 53-week-old aging mice was investigated. The results showed that OR could significantly shorten the destination quadrant distance and latency in the Morris water maze of aging mice. It obviously decreased the MDA, MAO-B and Lipo level in brain tissue, enhanced the SOD and GSH-Px activity in brain tissue, and increased the IgA, IgG, IL-1 and IL-2 level in serum. In addition, OR significantly improved the protein expres...
Zhongqing Wang1, Qiuyue Wu1, Ruiling Ye1, Fazul Nabi1,4, Yangfei Shang1, Sarfaraz Ali4 and Juan Liu1,2,3*
...in duodenum of diarrheal mice. A total 72 mice were randomly divided into 6 groups: control (CG), infection (IG), positive drug (PG), AMP low/middle/high dose (LG/ MG/ HG) groups respectively, (n=12). The mice were induced by diarrhea with E. coli (5×107CFU) via i.p injection (0.5 mL/time/d) after seven days of pre-administration except in CG. After the induction of the diarrhea, the...
Hye-myoung, Jang 1,3, Ju-Hyeun, Kim3, Garam Park1, Yoon Dong Choi4, Sun-Eui Kim1,5, Gwang Joo Jeon1,2*
...ted diseases through ICR mice experiment. The mice were fed with 5 mg of AE per day for 16 weeks to see the effect of AE on inhibition of two major biomarkers of beta-amyloid (Aβ) and tau-protein (τ-protein) formed in the brain tissues. Under the hypothesis of obesity inducing potential dementia, mice were fed with high-fat energy diet to gain excessive weight. The experimental gr...
Gamalat Y. Osman1, Elham M. Hassan1*, Tarek A. Salem2, Azza H. Mohamed1
...stosoma mansoni infected mice was studied in the present work and Praziquantel (PZQ) was applied as a standard drug. FWE was chemically analyzed by the high-performance liquid chromatography (HPLC) technique. The main recorded constituents were rutin, P-coumaric, hydroxyl-benzoic acid, ferulic acid, and sinapic acid. Results of the present study indicated that FWE (0.5 ml/mouse for 10 successive days) exhibited a significant reduction (P<0.05) in worm burde...
Rawda S. Mohamed1,2, Mohammed Y. Matoock2*, Abeer H. Abdelrazek2
...tress research. However, mice respond differently to stress. Therefore, we conducted this study to screen the behavioral and biochemical responsiveness of two different strains of mice, Balb/c (B) and Swiss (S). A total of fourteen adult males, each of (S) and (B) mice, were housed in either the control group or the multiple concurrent acute stress (MAS) group that was exposed to different...
Teguh Wahyono1,2*, Wahidin Teguh Sasongko3, Wijaya Murti Indriatama4, Setiawan Martono5, Slamet Widodo3, Widhi Kurniawan6, Muhamad Nasir Rofiq7
...detergent fibre (ADF), hemicellulose, cellulose, or non-fibre carbohydrate (NFC) content. Samurai 1 sorghum silage had the lowest NDF and ADF, both in non-wilted and wilted materials (P < 0.05). The interaction of wilting and different variety had a significant impact on NDF (P < 0.05), ADF, OM, and CP (P < 0.01). Wilting treatment had no significant impact on all aspects of in vitro degradability. In contrast, variety difference had a significant imp...
Taufiq Bachtiar1, Muftia Hanani2, Anisiyah2, Winda Puspitasari2, Wahidin Teguh Sasongko3, Teguh Wahyono4*
... acid detergent fiber, hemicellulose, and non-fiber carbohydrate. Organic matter, crude protein, ether extract, and non-fiber carbohydrate content in soil with pH 5.4 ranged between 91.94–94.67, 27.78–38.20, 13.99–22.78, and 16.31–33.82% DM, respectively. Meanwhile, in soil pH of 4.0, ranges were 92.24–94.80, 23.07–39.99, 14.88–22.82, and 12.69–38.20% DM, respectively. The Deja 2 genotype had the highest (p < ...
Hairul Islam M. Ibrahim1,2, Abdalla A. Sayed1,3, Abdel Naser A. Ahmed4,5 and Emad A. Ahmed*1,6
...ing in vitro and in vivo mice splenocytes and experimental autoimmune encephalomyelitis (EAE) in C57BL/6j female mice, respectively. The reduction of clinical symptoms and disease index were evaluated by histochemical analysis. T-cell population and cytokines levels in myelin oligodendrocyte glyco-protein (MOG) challenged PCB treated cells were estimated using flow cytometry and ELISA kits. Apoptotic and neuronal markers wer...
Jie Zhang, Na Li, Tingting Guo, Dandan Hu, Xiaofeng XU and Lili Zhang*
...ch are associated with hemicellulose degradation, and Desulfovibrio, which is associated with lactic acid oxidation, was significantly higher in the grazing than in the house-raised group (P < 0.01). The abundance of the acid-producing Enterobacter, Vibrio, and Selenomonas in the house-raised group was significantly higher than that in the grazing group (P < 0.05). The abundance of Prevotella_7 and Succinivibrionaceae-UCG-001 in the house-raised group wa...
Malik Fiaz Hussain1, Atif Akbar2, Muhammad Asif1, Laraib Nisar3, Muhammad Naeem Ashiq3 and Furhan Iqbal1*
...Ps on behavior of albino mice in a sex specific manner. Five weeks old albino mice (N = 48) of both sex were intraperitoneally injected with either low dose (20 mg) or high dose (50mg/ ml saline/ Kg body weight) of NiO NPs (average particle size 43 nm) for 14 days. Control groups were treated with saline solution. A series of behavioral tests were conducted in all subjects. Both low and high dose of NiO NPs treated male
Andi Hiroyuki1,2,*, Refina Sastya Qomariyuti1, Najmah Nur Islami3, Vanessa Ayu Sumirat4, Neni Anggraeni5, Mas Rizky A.A Syamsunarno2,6
...ematological profiles of mice. The study used 24 male mice of the DDY strain which were divided into three groups, namely the control group (Na-CMC-1%), KSO (moringa seed oil), and Omega-3 (fish oil). Mice were checked for temperature, GDP, and body weight at the beginning and end of the experimental period after 6 weeks. After that, the mice were sacrif...
Sajida Batool*, Sitara Shameem, Kainat Malik and Aneela Iram
... the liver and kidney of mice. Forty adult male mice were randomly divided into four groups each of 10: Control group was given normal untreated feed, Direct group was fed on food pellets exposed to microwaves in oven tray directly, Glass group was given feed processed in the microwave oven within a glass container and Plastic group was given feed exposed to microwaves in a plastic container. The microwaves exposed feed of a...
Bnar Shahab Hamad1, Bushra Hussain Shnawa1*, Rafal Abdulrazaq Alrawi2
...ysts infection of BALB/c mice was established, and the manifestation of immune actions and apoptosis were detected histopathologically and by immunohistochemical markers staining. The infected livers with hydatid cysts from the mice were processed for routine paraffin block technique. The sections of infected livers that were embedded in paraffin underwent immunohistochemistry. Several immunolabeled liver sections with anti-...
Rani Winardi Wulan Sari1, Novirman Jamarun2*, Suyitman3, Khasrad4, Elihasridas2, James Hellyward5, Gusri Yanti1
... (NDF), cellulose, and hemicellulose of 62.82%;65.69%;66.17%;50.67%; 57.22%; 55.84%; 63.27%. VFA and NH3 is 156.20%;9.84%. The methane gas production in P3 was lowest 20.57 ml/gr DM. This research concludes that P3 (40% hay mangrove leaves + 60% native grass) had the best resulted effect on nutrient and fiber in vitro digestibility, rumen fluid characteristic, and gas production.
Keywords | Hay mangrove leaves, Rhizophora apiculata, Native grass, In vi...
Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1
...ed by carrageenan in the mice model. Mice were induced edema via λ-carrageenan (50µl/ mice) and different concentrations of sericin (0.03 mg/ml, 0.06 mg/ml and 0.09 mg/ml) and curcumin (1%, 2%, and 3%) were administered to the respective groups, then blood was collected for hematological analysis to observe the mean count of erythrocytes, hemoglobin, platelets, white blood cel...
Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1
...ed by carrageenan in the mice model. Mice were induced edema via λ-carrageenan (50µl/ mice) and different concentrations of sericin (0.03 mg/ml, 0.06 mg/ml and 0.09 mg/ml) and curcumin (1%, 2%, and 3%) were administered to the respective groups, then blood was collected for hematological analysis to observe the mean count of erythrocytes, hemoglobin, platelets, white blood cel...
Shahid Iqbal1,2, Zulha Javed1, Mushtaq Hussain Zahid3, Qurat ul Ane Gillani4 and Furhan Iqbal1,*
...ood serum of male albino mice. The extracts were administrated orally (100 mg/ ml solvent/ Kg body weight) for 15 days and it was observed that the complete blood count (CBC) remained unaffected while cholesterol (P = 0.03) and triglyceride (P = 0.008) levels in serum were significantly elevated in P. pinnata leaf extract treated mice. Mice treated with E. camaldulensis leaf extract had si...
Maha Mostafa1, Sohail Soliman1, Reham I. Mohamed2 and Wael M. El-Sayed1*
...) and female (40) albino mice were orally administered testosterone at 25 mg/kg for 35 days. The males were allowed to mate with females to estimate the fertility index. After the end of treatment, mice were sacrificed, blood was collected for biochemical analysis, and sex organs were dissected out for histological preparations. Administration of testosterone has significantly increased the body weight and decreased the gona...
Chunlan Shan1, Chaoying Liu1, Qin Lu1, Guowen Fu2, Syed Aftab Hussain Shah3, Rana Waseem Akhtar4, Ru Zhao2, Libo Gao2, Chang Liu2, Shushu Miao2, Hongdan Wang2 and Hong Gao2*
...sinia. Later on, Kunming mice were infected with E. coli HPI+ and HPI- strains, respectively for the histopathology examination. The higher organ damage was observed by E. coli HPI+ than HPI- strain in mice. This study postulates the predicted protein structures of major genes, and validates the HMWP expression under iron starvation and provides a theoretical basis for prevention and treatment of E. coli related diseases.
Hongfeng Guo
...tudy, sixty premature SD mice were randomly divided into blank control group (n = 20), intermittent hypoxia group (n = 20) and drug administration group (n = 20). Establishment of intermittent hypoxia model: the oxygen concentration in the cabin of mice in intermittent hypoxia group was maintained at 40%, then decreased to 10% and maintained for 3 min. After that, it was continuously increased to 40%, and was decreased to hy...
Rinat Islamov*, Dinara Turegeldieva, Altyn Rysbekova, Kuralay Sarmantayeva, Victor Semenuyk
... samples from laboratory mice and rats were realtime polymerase chain reaction and enzyme-linked immunosorbent assay. A high prevalence of some pathogens among conventional laboratory animals used by some academic institutes and universities of Almaty has been shown. Rodentibacter pneumotropicus has been detected in the SPF animals, while no other pathogens from the FELASA list have been detected. The obtained results show the importance of not only regular he...
Aamir Mushtaq1*, Fatima Habib2, Umar Farooq Gohar3, Abdul Malik4 and Mobasher Ahmad2
...rformed in female albino mice by administration of single oral doses (4, 5, 6, 7 and 8 g/Kg/po) respectively, to individual groups (n=5) and after that the behavioral changes were observed. To find the chronic toxicity, the animals were divided in three groups (n=6) which were orally administered with normal saline, P. anisum methanolic extract 400 mg/Kg, and 800 mg/Kg respectively for three months. The results of acute toxicity study represented the LD50, val...
Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1
...oglitazone (Pio) against mice bearing Ehrlich solid carcinoma in terms of its ability to inhibit cell proliferation and angiogenesis. Six groups were created using sixty male mice after the Ehrlich inoculation tumor growth was confirmed: control, Cis (5 ml/kg), Pio low dose (LD) (17.5 mg/kg), Pio high dose (HD) (30 mg/kg), Cis+Pio LD, and Cis+Pio HD. Different parameters, including behavioral, biochemical such as malondialde...
Ali I. Al-Ameedi1, Zahraa M. Ayad2*, Wed Abbas Mohammed3, Salim K. Hajwal4
...>. Forty male albino mice were randomly divided into 4 groups of ten animals. The first group received hydroxyurea (80mg/kg B. W) orally daily for 30 days, while second and third group received Ginkgo Biloba (100 mg/ kg.BW) and omega3 (150 mg/kg.BW) orally daily for two weeks of HU administration as protective and 4th group (C-ve) were considered as control negative group was given distilled water orally. After therapy by removing the bone marrow from the ...
Yuhua Chen1 and Song Lu2*
...elated T cells. C57BL/6J mice used in the study were randomly divided into control and electrical stimulation group. The tumor model was established by subcutaneously injecting MC38 colorectal adenocarcinoma cells into mice. The mRNA expression of pro-inflammatory factors IL-10, IL-6 and 1L-1β of the mice was analyzed by RT-PCR. The numbers of CD8 + T cells and CD4 + T cells were anal...
Zhe Lin1, Yumo Li1, Yuchen Wang1, Yuechen Li1, Ke Pei1, Guangfu Lv2, He Lin1* and Zhun Yu3*
...erify this, total 96 ICR mice were used, forced swim test and anoxic tolerance test were performed. The effect of OR on levels of monoamine neurotransmitters and phosphorylation of p38 which closely related to anti-stress were examined. The results showed that, 203 potential OR targets and 126 stress-related gene targets were obtained, in which there were 15 common targets. Pathway enrichment analysis showed that there were 20 critical pathways. The results re...
Muhammad Shuaib1*, Abdul Hafeez1, Ziaul Islam2, Assar Ali Shah3 and Saad Ullah1
...r, ash, cellulose, and hemicellulose content for diets containing soybean hull (SH) at 3, 6, and 9% compared to the basal diet. Egg weight and egg quality parameters during different phases were not affected among the treatment groups. Total revenue and profit during all phases (phases 1, 2, and 3) while cost-benefit ratio during phase-1 and 2 were recorded significantly higher for the control group than all other treatment groups.It is concluded that the effe...
Waheed Khan1, Muhammad Ajmal Khan2, Bakhtawar Khan1*, Aftab Amin3, Muhsin Ali4, Awais Farid, Ali Hazrat5 and Muhammad Yahya5
...here were five groups of mice (n=10): group 1 (normal control) received normal food and water, group 2 (diabetic control) received normal food and clean water, group 3 (diabetic mice) received 200 mg of methanolic plant extract, group 4 (diabetic mice) received 400 mg of methanolic extract, and group 5 (diabetic mice) received 10 mg of the medication Gli...
Sen Ye1, Jiangang Pang2 and Xiuli Wang3*
...s cerebral hemorrhage in mice with TPA ischemic stroke after thrombolysis. Male Wistar mice (11-week-old) as our experimental subjects were administered with warfarin (0.2 mg/kg/day), rivaroxaban (60 mg/kg/day), rivaroxaban (120 mg/kg) /day), or solvent pretreatment for 14 d to induce transient middle cerebral artery occlusion for 90 min, followed by perfusion with tPA (10 mg/kg/10ml). Infarct volume, bleeding volume, immuno...
Xiaowei Huang1, Chao Ma1, He Lin1, Yuchen Wang1, Yan Xu1, Guangfu Lv2* and Zhe Lin1*
...ncer Research (ICR) male mice were divided into control, chronic unpredictable mild stress (CUMS), fluoxetine (3 mg/kg), OR800 (OR 800 mg/kg) and OR400 (OR 400 mg/kg) dose groups. After the last administration, the content of corticotropin-releasing hormone (CRH), adrenocorticotrophic hormone (ACTH) and corticosterone (CORT) in serum were detected. Hematoxylin and Eosin (H&E) staining was used to observe the hippocampal histomorphological changes. The prot...
Aamir Ali, Hafiz Muhammad Tahir*, Azizullah, Shaukat Ali, Muhammad Summer, Ali Haidar Gormani, Saira Nawaz and Ayesha Muzamil
...formalin was injected to mice paw for induction of pain. These extracts significantly decreased edema in mice paw. The level of TLC and RBCs was increased to normal. Platelets level was also increased in mice treated with mulberry leaves extracts. Furthermore, these extracts significantly reduced the inflammatory pain and licking response triggered by injection of formalin in
Zahra Abdulameer Al-Zayadi1, Hana Kadum Shanan1, Al Salihi Karima Akool2*
...yrrolidone K-30 (PVP) in mice. 72 mice divided randomly into eight groups (G1, control group; G2, Sc-RE group; G3, chemotherapy alone; G4 cancer- group without treatment, G5, cancer-induced + chemotherapy; G6, cancer induced+ Sc-RE/ low dose; G7, cancer induced+ Sc-RE/ moderate dose; G8, cancer induced+ Sc-RE/ high dose). Liver and kidney samples were collected from all mice during differe...
Isatay Tusupovich Jakupov, Gulzhan Tursunovna Yeszhanova, Gulnur Kurbanalievna Mamytbekova*
...was carried out on white mice using 10%, 20%, 40% concentration of C. leucocladum, no death of mice was recorded within 30 days. When studying the irritating and allergic effects of C. leucocladum on rabbits, it was found that no irritating effect and allergic reaction was observed within 24 hours in animals with 10%, 20%, 40% content of C. leucocladum. Studies of the antimicrobial properties of C. leucocladum extract with 1...
Dede Kardaya*, Deden Sudrajat, Dewi Wahyuni
...ergent fibre (ADF) and hemicellulose digestibility (P<0.05), and increased feed efficiency and live weight gain of lambs (P<0.05). It is implicated that zeolite or urea-impregnated zeolite improves lamb performances probably as a result of the cation exchange capacity of zeolite or slow-release characteristics of urea-impregnated zeolite. ...
Elihasridas, Mardiati Zain*, Roni Pazla, Simel Sowmen, Qurrata Aini
... fiber, cellulose, and hemicelluloses in the ammoniated aromatic lemongrass waste samples was evaluated by incubating them on in-vitro media at 39ºC/48 h. The analysis of variance following a 5 x 4 randomized block design was used. The results revealed a significant (P<0.05) increase in the digestibility of ammoniated aromatic lemongrass waste in the rumen. The degradation of ammoniated aromatic lemongrass waste was found to be significantly (P<0.0...
Azza M. El-Kattawy1, Tarek Abou Zed1, Randa Megahed1 and Mohammed El-Magd2*
...tis. Female Swiss albino mice (20-30g) were randomly divided into 5 groups (n= 10/group): control, Sim control, pristane-induced arthritis, Sim co-treated, and Sim post-treated group. Sim treatment significantly 1) downregulated the expression of immunomodulatory genes [interferon gamma (IFNγ) and lactoferrin (LF)], 2) decreased the expression of inflammation-related genes [tumor necrosis factor alpha (TNFα) and interleukin 1 beta (IL1β)], 3) ...
Zhipeng Song1,2, Jialiang Xin1,3, Xiaoli Wei1,2, Abula Zulipiya1,3, Kadier Kedireya1,2 and Xinmin Mao1,3*
...ffect of marein on db/db mice and its mechanism of action intervening in diabetic retinopathy. For in vivo experiment: 10 healthy db/m mice were used as the control group. Forty healthy db/db mice were randomly divided into the model, positive, ranibizumab and marein groups. There were 10 mice in each group. All mice w...
Bilal Ahmed Shah1, Muhammad Avais1*, Jawaria Ali Khan1, Masood Rabbani2, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Muhammad Awais1, Sohail Ahmad3 and Shahan Azeem2*
...hen injected either into mice or rabbits or cow calves. Challenge protection studies revealed a significantly higher survival rate in vaccinated mice and rabbits compared to placebo groups. None of the vaccines when streaked onto culture media showed any growth indicating sterility of the vaccines. Repeated measures ANOVA was applied to compare mean O.D values of treatment groups for evaluation of humoral immunity. Serum ELI...
Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3
... was evaluated in BALB/c mice. The expression of recombinant OmpH protein (rOmpH) resulted in its accumulation in cytosol. The purified rOmpH protein had a molecular weight of 36 kDa and concentration around 0.973 mg/mL. The BALB/c mice group injected with 100 ng of purified rOmpH exhibited significant protection (80%) when challenged with LD50 of P. multocida B:2. From the obtained results it is evident that rOmpH can be us...
Dan Song1,2, Ming-Juan Ge3, Jie Li1,2, Yi Jiang1, Xiu-Mei Kong1, Jiao-Jiao Xu1, Xu Ji1,2, Rui-Xin Shi1 and Qin Zhao1,2*
...ific pathogen-free (SPF) mice were randomly divided into blank control, model (hypoxia), positive (propranolol 30 mg/kg + hypoxia), and three G. straminea ethanol extracts dose groups (10, 5 and 2.5 g/kg respectively + hypoxia), administered intragastrically once a day for 14 consecutive days. After that, multiple hypoxia experiments were conducted including soda lime normobaric hypoxia test, sodium nitrite poisoning test, isoproterenol poisoning test, and acu...
Usama Mahalel1, Barakat M. Alrashdi1, Ibrahim Abdel-Farid1, Sabry El-Naggar2, Mohamed Hassan3, Hassan Elgebaly1 and Diaa Massoud1,4*
... tetrachloride (CCl4) in mice at two different doses (low; 100 mg/kg BW and high; 500 mg/kg BW). The obtained results showed that CCl4 injection significantly reduced the body weights of mice and increased the relative liver weight. Treatment with M. forsskalii extract after CCl4 injection restores the weights of mice to the normal range and reduced the relative weight of the liver.
Johan Sukweenadhi1*, Stefan Pratama Chandra1, Finna Setiawan2, Christina Avanti2, Kartini Kartini2, Arief Koeswanto3 and Deok-Chun Yang4
...H scores in the diabetic mice group that were given a dermal patch formulated with silver nanoparticles synthesized using Plantago major L. extract were not significantly different from the normal group and the ACA and ACA Ag groups. Based on these results, it can be concluded that the dermal patch formulated with silver nanoparticles synthesized using Plantago major L. extract helps wound healing in diabetic mice<...
Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy
...HK21 cell lines and baby mice. Identification was done by both Indirect Fluorescence Antibody Technique (IFAT) on tissue culture and inoculated mouse brain, while immunoperoxidase technique was done on bovine lung. Haematological examination revealed leucocytosis accompanied with neutrophilia (immature form) and lymphopenia. Histopathological examination clarified the most common lesion of field cases in lung in the form of pulmonary emphysema and alveolar col...
Eman, M. M. Soliman*; Taha, M. M.*;El-Sanousi A,;Shalaby M. S. Wasscl , Youscf Adel*
...raperitoneally(I/P) into mice (3-5 days old) and lambs without showing adverse post vaccinal reactions. Duration of immunity to RVF virus in vaccinated sheep has been determined by using both serum neutralization test (SNT) and enzyme-linked immunosorbent assay (ELISA) on sera collected weekly up to 7 weeks post vaccination, where the antibody titer elevated from I st WPV till reach the protective level at 5th WPV. Effective doses in
Rehan Ahmed Siddiqui1,2*, Shabana Usman Simjee2,3, Nurul Kabir4, Muhammad Ateeq3,5, Kevin Joseph Jerome Borges6, Muhammad Raza Shah3 and Rahman M. Hafizur2
...s-induced AKI induced in mice. CA (50 mg/Kg) and CA-AuNPs (30 mg/Kg) were given to the animals for four days. On the fourth day and after 24 h of water deprivation, 50% glycerol intra-muscular was administered. Blood urea and creatinine were tested. Kidney histopathology was done and damaged kidney areas were measured. Immunohistochemistry for actin and cyclooxygenase-2 (COX-2) were also carried out. Real time RT-PCR studies were performed to check mRNA expres...
Salema Lafta Hassan1*, Taghred Jabbar Humadai1, Sabrin Ibraheem Mohsin2
...tological status of male mice testes. Eighty adult Swiss Albino mice were separated into four groups at the age of two months. The first group (G1) was given Folcord (500 mg/kg) orally daily for four weeks, whereas the second group (G2) was given Folcord as well as I/P inoculation with 0.1 ml oil adjuvant vaccine (OAV) of Pasteurella multocida twice a week for two weeks. The third group was vaccinated by I/P with 0.1 ml oil ...
Suhad Mohmmed Alrobyrai1 , Falah Muosa Al-Rekabi2*
...model. Fifty male Balb-c mice were divided into five groups. Groups received 700 mg/kg.BW of APAP and oral treatments: G1 (distilled water, negative control), G2 (no treatment, positive control), G3 (Par+Tempol, 20 mg/kg.BW), G4 (Par+ALA, 20 mg/kg.BW), G5 (Par+Tempol+ALA, 10 mg/kg.BW each). Half animals were sacrificed after 30 days, the rest after one week of treatment withdrawal. Investigation included body weight, biochemical markers (ALT, GGT, PT, INR), an...
Ahmed K. Fahd*, Ali H. Saliem
...igenin and Diclofenac in mice model. In the beginning, 84 male albino mice were selected, acclimated, and categorized into four equal groups: The 1st tested by the hot plate, the 2nd treated with acetic acid, the 3rd examined by application 10 μl of 2% formalin solution and the 4th was examined by the tail-flick test. Within each experiment, the study mice were subdivided equally into t...
Sijie Jian1,2,3,4, Wei Sun1,3, Jia Chao1,2, Na Rong1,4, Xiang Liu1,2,3,4* and Chen Chen1,2,3,4*
...tions were optimized. In mice immunized with Slp, the immune-related factors of LZM and AKP were enhanced (p < 0.05), and a specific antiserum titer (1: 3200) was obtained that had immune recognition effects for A. hydrophila and P. fluorescens. Passive immunization of Carassius auratus with Slp mouse serum and challenging with bacteria showed a passive protection rate of Slp serum against A. hydrophila infection of 42.5 % (p < 0.05) and a passive cross-...
Shamsa Jabeen and Javed Iqbal Qazi*
...H levels in the serum of mice following extensor digitorium longus (EDL) muscle grafting. Blood from the intact control as well as experimental and EDL muscle grafted mice was secured on day 3, 5, 7 and 14 post-transplantations. CK activity of control and EDL muscle grafted mice with probiotics showed 157% and 130% increase, respectively on day 5, whereas on 14th days post-transplantation ...
Rondius Solfaine1, Faisal Fikri2, Salipudin Tasil Maslamama3, Muhammad Thohawi Elziyad Purnama4, Iwan Sahrial Hamid5*
...the pulmonary section of mice induced with canine coronavirus (CCoV) vaccine. The research utilized male mice (Mus musculus) aged two to three months, divided into three groups: a control group administered with normal saline (D0), a CCoV-induced group (D1), and a group induced with CCoV along with 1 mL/kg body weight of Lactobacillus acidophilus for seven days (D2). The CCoV vaccine was administered subcutaneously at 0.5 mL...
Kashif Abdaal, Aneeza Batool, Muhammad Tariq Navid*, Saeed Ahmed, Asma Saleem Qazi, Waseem Safdar, Haidar Ali, Mobeen Ur Rashid, Somia Rafaqat
...rious model animals like mice, ferrets, pigs, and nonhuman primates before using them for human trials. The other side of successful vaccine intervention, however, contains various issues that need to be rectified. These concerns primarily depend on target pathogen selection, novel vaccine efficacy, development, and duration of optimum immunological responses, selection of appropriate animal model, need for booster dose, route of administration, and post-admin...
SHAGUFTA ANDLEEB1*, ASMATULLAH2, SHABANA SHAUKAT1 & SIDRA NAWAZ2
...ical changes in liver of mice. Twenty mice (4-5 weeks old) were given oral gavages of 0.1 ml of 0.00, 31.25, 125 and 500μg/g B.W (Complete). of Metformin prepared in distilled water, for 04 weeks daily. After 24 hours of last dose, the animals were anaesthetized torecoverliver tissues following fixation in Bouin’s fluid and processing for histological preparation using different grades of alcohol and xylene for dehy...
SYEDA NADIA AHMAD1, MEHWISH NASIR1, AQSA NAUREEN1, SHAGUFTA ANDLEEB4, KAUSAR RAEES3, TAHIR ABBAS2, ASMA YOUNIS1 & KHAWAJA RAEES AHMAD*1
... 20 mgkg-1 in adult male mice (10 in each dose group) for thyroid and parathyroid histopathology. Tissues were recovered following 48 h of DZ treatment. Micrometric data in terms of follicular size of thyroid, cellular, nuclear sizes of the follicular and chief cells were obtained from the histological sections. Statistical analysis has shown significant (p<0.001) variations in mean follicular size of thyroid indicating dose dependent depletion of colloid (...
SYED SHAHID IMRAN BUKHARI1*, IQRA AZAM1, MUDDASIR HASSAN ABBASI1, MEERAB DAUD1, NADEEM SHEIKH2, ANDLEEB BATOOL3, RABIA MAHMOOD2 & MARYAM MUKHTAR2
... cakes etc. Swiss albino mice of six weeks were divided into control group and experimental group. Experimental group was administered with Alloxan monohydrate (200 mg/kg) intraperitoneally and then livers were extracted after 3, 6 and 12 hours. All the samples were processed for gel run and thermo cycler program, which revealed alterations in the expression of hepatic mRNA (IL-6) in all loads equally confirmed by the results of housekeeping gene β-actin....
Marah Salim Hameed1, Ali Ibrahim Ali Al-Ezzy2*
...nd E -sodium selenite in mice intoxicated with sodium nitrate. Thirty healthy adult male mice of 25-30gm body weight divided randomly into six groups (5 mice/each) administered the following daily for 2 weeks, respectively: Vitamin E -sodium selenite 0.5 ml/LDW(T1); vitamin C (0.5 gm/L (T2); NaNO3 0.5gm/L (T3); NaNO3 (70 mg/kg) and vitamin E -sodium selenite 0.5 ml/L (T4); NaNO3 [70 mg/kg ...
Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1, and Rida Haroon Durrani1
... and clearance in BALB/c mice. The B. abortus ΔpurD mutant exhibited significant attenuation of virulence and cleared from the mice spleen after 20th DPI when inoculated at the dose of 108 CFU per mice. In contrast to this, the parent RB51 strain induces splenomegaly and showed higher persistence with significantly higher splenic CFUs in the mice g...
Yan Wang1,2*, Lin Liu3, Na Li4, Yijun Zong1, Wei Liu1, Wenhua Xu1, Qi Wang1, Peijuan Zhang1 and Huiling Feng2,5*
... of lung cancer in naked mice were both noted. The effects of LY6K knockdown on glucose uptake rate, lactic acid and energy metabolism of A549 cells were determined by UV spectrophotometry. According to data analysis from GEPIA2, TCGA, and Kaplan Meier plotter, lung adenocarcinoma exhibits high levels of LY6K expression and is associated with a poor prognosis. The expression of LY6K was most significant in A549 cells. In A549 cells, LY6K knockdown significantl...
Featuring
-
LY6K Promotes Proliferation and Energy Metabolism of Lung Adenocarcinoma Cells by Regulating Aerobic Glycolysis
Yan Wang, Lin Liu, Na Li, Yijun Zong, Wei Liu, Wenhua Xu, Qi Wang, Peijuan Zhang and Huiling Feng
Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1337-1344
-
Modulation of Tau Expression in the Colonic Muscle Layer of a Rat Model with Opioid-Induced Constipation
Jinzhao Li, Yawen Zhang, Binghan Jia, Yuqiong Zhao, Huijuan Luo, Xiaojie Ren, Yuan Li, Xiaoyan Bai, Jing Ye and Junping Li
Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1323-1335
-
Agricultural Habitat Heterogenity Impact on the European Hare (Lepus europaeus) Density in Vojvodina Region (Serbia)
Igor Ponjiger, Milutin Kovacevic, Vladimir Markovic, Zoran Ristic and Vukan Lavadinovic
Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1315-1321
-
Application of UAV Image Detection Based on CBPSO Algorithm in Crop Pest Identification
Li Tian, Chun Wang, Hailiang Li, Haitian Sun and Chang Wang
Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1305-1314
Subscribe Today
Receive free updates on new articles, opportunities and benefits
Interact with ResearchersLinks
Guidelines and Support
ResearchersLinks Initiatives
Information and Help
Follow Us
 |
 |
 |
 |
© 2024 ResearchersLinks. All rights Reserved. ResearchersLinks is a member of CrossRef, CrossMark, iThenticate.