Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
...rotein in the nucleus of oocytes which can promote in vitro nucleosome formation. In this study, to improve the efficiency of SCNT in Banna Mini-pig Inbred Line (BMI), we obtained the recombinant nucleoplasmin (Npm) by using a prokaryotic expression system and compared the difference on the developmental effects between exogenous Npm and its structural analog, polyglutamic acid (PGA). We chose the pMD18-T vector for Npm expression and transformed E. coli BL21 ...
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*
...d that LET could inhibit oocyte growth in female and promote the development of testes in males. For the gonad differentiation related genes, the expression profiles of Cyp19A and Cyp19B show a pattern of increase in short time and low concentration, but decline in long time and high concentration. Arα has a trend of increases, ERα shows downwards trend. Dax and Foxl2 are female-biased genes, their expression profiles show simil...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
...d supplementation on the oocyte in vitro maturation and early embryonic development after in vitro fertilization. Sheep follicular fluid (SFF) was collected from different ovaries and supplemented to the in vitro maturation (IVM) media with proportions 0%, 10%, 20% and 40%. Oocyte maturation was assessed in different treatments and then cumulus-oocyte complexes were us...
Şehriban Çek Yalnız 1,* and Erdal Yilmaz2
...lasmic organelles of the oocyte were disintegrated. The theca of atretic mature oocytes was hypertrophied and persisted to form the interstitial cells, whereas the granulosa cells were regressed and disappeared. Erythrocytes and leukocytes were also detected at the advance stage of atresia. Apoptosis in the granulosa cells were clearly detected. The chromatin condensation against the nuclear envelope, cell shrinkage, surface...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
... reproduction (IVF). The oocytes are inevitably ruptured by the microinjector in the process of microoperatin. To study the effects of mechanical damage from microopetations on embryo development, we analyzed 60 the spent culture media of porcine parthenogenetic embryos experienced different types of microopetation using Raman spectroscopy. The results showed that Group II with the puncture of egg zona and plasma membrane had the highest cleavage rate and blas...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
Abd El-Nasser Ahmed Mohammed1,2,*
...iological parameters and oocyte quality after ovarian transplantation upon dietary supplementation of 1.0 and 2.0% of Nigella sativa (N. sativa) oil to female mice and secondly to explore if dietary N. sativa is effective in alleviation of hypothermia and hyperglycemia due to general anesthesia. Seventy-five albino female mice (21.74 ± 0.19) were distributed into three groups; control (G1; N=25; not receive N. sativa oil) and...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
...d breaks (DSBs) in mouse oocytes and resultant early embryos. Germinal vesicle (GV) oocytes were first cultured to monitor the progression of germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) during in vitro maturation (IVM), then the harvested metaphase II (MII) oocytes were parthenogenetically activated to evaluate pronuclear (PN) formation of parthenogenetic embryo...
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
...esis, follicular growth, oocytes maturation, ovulation and corpus luteum formation. The aim of this study was to identify the polymorphisms in the exon 11 of bLHR/HhaI gene (SNP g.1553C>T) and its association with service per conception (S/C) of Pasundan cows of West Java, Indonesia. Total of 147 Pasundan cows were used in this study. The PCR-RFLP analysed three genotypes of CC (0.11), CT (0.57) and TT (0.32) were detected in the Pasundan bLHR/Hha...
Arslan Mahmood Ahmad1, Muhammad Ameen Jamal1, Abdul Sattar1, Aamir Ghafoor2, Aqeel Javeed3 and Amjad Riaz1*
...Artificial activation of oocyte is a one of key step in cloning. Strontium chloride (SrCl2), a calcium oscillation inducing salt, is commonly used for the artificial activation mouse oocytes; however, limited reports are available to evaluate its negative impact. The objective of this study was to optimize the concentration of SrCl2 for artificial activation of mouse oocyte
Zhi-Peng Li, Laiba Shafique, Saif ur Rehman, Kui-Qing Cui, Xiao-Can Lei, Xing-Rong Lu, De-Shun Shi* and Qing-You Liu*
...Gas6) during porcine oocyte maturation and early embryonic development were systemically investigated. The expression of Gas6 in the process of follicular development was studied by immunohistochemical staining, immunofluorescence, QRT-PCR and western blot. Immunohistochemical staining results showed that Gas6 was expressed in the oocytes, especially the nucleus, from the primary to preovulatory stages. The furthe...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3*, Abd El-Nasser Ahmed Mohammed3
...evelopment stages on the oocyte and the surrounding cumulus cells to prepare the oocyte for further embryo development upon ovulation and fertilization. Molecules transfer to oocytes through gap junctions of cumulus cells. Therefore, cumulus cells and their secretion, follicular fluid, effect on oocyte maturation and developmental potential of the result...
Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1
...gative cows. The intact oocytes were clearly pronounced only in the negative animals. The fallopian tubes are free from any pathological lesions in the negative animals. Immunohistochemical, BVDV antigen was detected in ear notch, ovaries, fallopian tubes and uterus of one cow (1/30) (PI animal). Whereas, BVDV antigen was detected only in the genital organs of acutely infected animals (2/30) but ear notch was negative. The positive BVDV antigen was clearly ob...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3, Abd El-Nasser Ahmed Mohammed3, Al-Hassan Mohammed4*
...vivo or in vitro include oocyte meiotic maturation, fertilization and culture processes. Oocyte maturation is the first step for in vivo or in vitro embryo production. Oocyte maturation appears to be a simple step, but it is a very complex process. Maturation of oocytes include several changes at the cytoplasmic and nuclear levels that makes the
Abd El-Nasser Ahmed Mohammed*, Aiman Al Mufarji and Sultan Alawaid
...ial follicles consist of oocytes surrounded by a single layer of pregranulosa follicular cells and they remain dormant in the meiotic prophase I stage. Primordial pregranulosa follicular cells initiate activation of primordial follicle and govern the development of dormant oocytes. The primordial follicles take about 6 months to grow and develop to ovulatory graafian follicles in cattle and humans. Growth of preantral follic...
Othman E. Othman1*, Lingjiang Min2, Amira M. Nowier3
... in folliculogenesis and oocyte ovulation rate. Also, this study declared the opposite association between the methylation levels and expressions of differentially methylated genes which are related to fertility phenomena in goats.
Keywords | DNA methylation, Ovulation rate, Litter size, WGBS, Goat.
...
S. Riffat†, S. Kumar and A. Soomro
...gnificant suppression of oocytes and testis development in infected individuals....
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
... to their importance for oocyte maturation, artificial oocyte reprogramming, embryo development and their resulting newborns.
Keywords | In vitro, Oocyte, Embryo production, Embryo transfer, Micromanipulation
...
Chalothon Amporn1, Somchit Guntaprom1*, Sarawut Duongmawong1, Wilasinee Srisanyong1, Sirikanda Thanasuwan1, Phalita Koonnadilokpot1, Dechawut Bunyaluk2, Juggrid Jugsumrit3, Jakrit Yaeram4, Chompunut Lumsangkul5
... Experimentally, cumulus-oocyte complexes (COCs) which were obtained from bovine ovaries underwent culturing for a period of 22 h in TCM-199 containing an additional 10% fetal bovine serum, streptomycin (100 μg/ml), penicillin (100 U/ml), 25 μg/ml FSH, 2 IU/ml hCG, 0.2 mM sodium pyruvate, 1 μg/ml 17-β-Estradiol, and either 0, 100, or 200 μM/ml cysteine where 0 served as the control. IVF-TALP medium was used to fertilize the mature
M.A.M.M. Shehab-El-Deen1,2*, S.A. Al-Dobaib1 and K.A. Al-Sobayil1
...s produced in vitro from oocytes matured in stressful conditions of elevated concentrations of non-esterified fatty acids (NEFAs). The maturation conditions in the current study were resemble the situation in the oocyte microenvironment of metabolically stressed lactating dairy cows during the post-partum period where the negative energy balance occurred. Oocytes were in vitro matured unde...
Beenish Shahid1* and Muhammad Ijaz Khan2
...er recovery of competent oocytes in buffalo species limits the commercialization of in vitro embryo production technology in field conditions. The present study aimed to improve the cytoplasmic and nuclear maturation of in vitro matured oocytes of Nili Ravi buffalo in the presence of hormones. The denuded oocytes (DOs) obtained by repeated pipetting were collected from 2-8mm follicles. A s...
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
... slaughterhouse and were oocytes mature before fertilization using semen from one bull, oocytes are fertilized then cultured for 48 hours. Embryo grouping based on cell count was conducted after 48 hours of culture and was recultured for 48 hours. Follicular fluid, oocyte maturation media and embryo culture media were collected using a disposable syringe and centrifuged, then supernatant i...
Sri Rahayu*, Muhaimin Rifa’i and Widodo
...9), which is secreted by oocytes, is a paracrine factor that plays a vital role in follicular development. We performed a computational biology analysis to determine the role of GFD9 in mammalian ovarian function. We constructed a three-dimensional model of GDF9 using I-TASSER and human GDF9 protein sequences obtained from UniProt. We also analyzed GDF9 gene expression levels using GENEVESTIGATOR. Furthermore, we identified the proteins that interact with GDF9...
Erni Damayanti1, Herry Sonjaya2*, Sudirman Baco2 and Hasbi Hasbi2
...s produced in vitro. 542 oocytes were matured in TCM 199 medium, were fertilized and were cultured using CR1aa medium for 48 h. After 48 h of culture, the embryo developed into 2, 4, 8 and 16 cells. Furthermore, it was grouped based on the stage of development, each of which was re-cultured for 48 h. After that, its development was re-evaluated and H2O2 measurements were taken. The 2-cell embryo was not able to reach the morula, while stage 4-, 8- and 16-cell ...
Diky Ramdani1*, Dwi Cipto Budinuryanto1,2, Siti Darodjah Rasad1, Novi Mayasari3, Krisna Rizki Rismawan1, Muhammad Fajryanto Solihin1, Ririn Siti Rahmatillah1
...ory. Additionally, fecal oocyte counts (FOC, eggs/g feces) of Coccidia were consistently lower for PPS-50 and PPS-75 compared to PPS-0. After the anthelmintic treatment, dietary PPS-75 significantly increased (P < 0.05) the ADG of sheep without adversely affecting the total DMI compared to the control group. This suggested that PPS had the potential to serve as both a feed supplement and an anthelmintic agent.
Keywords | Cocci...
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