Muhammad Shah Zaman1, Ghulam Muhammad Ali2, Aish Muhammad1, Khalid Farooq2, Iqbal Hussain1*
...obacterium-mediated gene transformation of AtNHX1 to improve their tolerance against salinity stress....
Ummara Waheed Khan, Raza Ahmed, Irum Shahzadi and Mohmmad Maroof Shah
Email | mmshah@ciit.net.pk
... tissue culture, genetic transformation and in vitro regeneration offered new dimensions of research to improve important crop species including wheat. The tissue culture by itself has increased the crop improvement potential to develop new cultivars with desirable traits through somaclonal and gametoclonal selection. While successful tissue culture is critical for genetic transformation, it has been faci...
Muhammad Zafarullah Khan
...o drastic changes in the transformation of agriculture to produce more farm commodities in shortest possible time with minimum inputs. Agriculture is the pivotal source of producing food crops including dairy, fruit cultivation, poultry, bee keeping and edible and non-edible like forestry products etc. An Agriculture Extension Officer (AEO) plays the role of a hub in agricultural development and covers all the areas of Agriculture. The present study was carrie...
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...ollination and transient transformation efficiency decreased at 22 days after pollination. Enhanced transient beta glucuronidase (GUS) expression with average frequency of 40.56% was noticed with three days of co-cultivation period while two days of co-cultivation period resulted 24.07% transient GUS expression. Addition of 100 mgL-1 L-cysteine in co-cultivation medium increased the transient transformation by 33.9% compared...
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...ing material for genetic transformation. Unlike immature embryos, the development of coleoptilar node is seasonal free and green house independent which would accelerate the work relevant to genetic transformation of maize. MS medium fortified with 3 mgL-1 BAP and 5 mgL-1 picloram produced maize seedlings with expanded coleoptilar nodes. Longitudinal cut sections of expanded coleoptilar nodes produced maximum primary calli o...
Gamalat Osman1, Mansour Galal1, Abbas Abul-Ezz2, Ahmad Mohammed1, Mohammed Abul-Ela3 and Asmaa Mostafa Hegazy2
...ation is due to their biotransformation process during metabolism. The histopathological observations of the gills and mantle of L. carinatus showed pronounced alterations at 24 hours and proliferated at the day 15 but showed a further decline at the day 30 of exposure. These findings seem to be time related and were classified as reversible and irreversible changes. Accordingly, it can be concluded that L. carinatusis a typical bioindicator for crude oil poll...
Ann Milliken Pederson
... for wholeness, healing, transformation, and an encounter with the ultimate. Ted Peters’ new book locates the vocation of humankind within their cosmic home, brings together the disciplines (humanities, social sciences, natural sciences) into a holistic integration of knowledge, places the quest and the question of God within the historical narrative of the natural and cultural history of the entire cosmos, and offers a vision of hope and action to creat...
Aqsa Waqar1, Sahir Hameed Khattak2, Sania Begum2*, Tayyaba Rehman1, Rabia1, Armghan Shehzad2, Wajya Ajmal2, Syeda Shahdana Zia2, Iqra Siddiqi2 and Ghulam Muhammad Ali2*
... wheat by pyramiding and transformation. This review discusses information regarding rust disease and resistance in wheat to tackle the disease through resistance breeding. ...
Tasawar Sultana*, Farah Deeba* and S.M. Saqlan Naqvi*
HIGH-THROUGHPUT AGROBACTERIUM-MEDIATED TRANSFORMATION OF MEDICAGO TRUNCATULA IN COMPARISON TO TWO EXPRESSION VECTORS
...nt Agrobacteriummediated transformation for a long time. The selection of Medicago truncatula as a model legume plant for molecular analysis resulted in the development of efficient Agrobacterium-mediated transformation protocols. In current study, M. truncatula transformed plants expressing OsRGLP1 were obtained through GATEWAY technology using pGOsRGLP1 (pH7WG2.0::OsRGLP1). The transformation
Ayesha Iftikhar1,*, Muhammad Asif Aziz1, Muhammad Naeem1, Munir Ahmad1 and Tariq Mukhtar2
...pectively. The values of transformational rate (Qp) exhibited that 25.62, 42.11 and 74.50 nymphs of P. solenopsis were required per female beetle to lay one egg at 24 ± 0.5 °C, 27 ± 0.5 °C and 32±4°C, respectively. Finite predation rate (ω) was more (7.64) at 24±0.5°C followed by 27±0.5°C (6.56) and the lowest at 32 ± 4 °C (0.66). Adult beetles proved strong natura...
Ayesha Noreen1, Dilara A. Bukhari2 and Abdul Rehman1,*
...nflammation and cellular transformation in cancer. A better understanding into the mechanism of ROS in cancer progression might be useful for the development of biomarkers and therapeutic strategies. The objective of this review was to summarize the roles of ROS in different stages of cancer, cell invasion, angiogenesis and metastasis.
...
Sabahat Noor*1, Shaukat Ali1, Hafeez-ur-Rahman1, Farhatullah2 and Ghulam Muhammad Ali1
...of lasani-08 from single transformation event was exposed to 15 days drought and different levels of salt concentrations separately. Four parameters i.e. sugar contents, proline contents, chlorophyll contents and relative water contents were recorded and compared in transformed and non-transformed plants. It was observed that under drought and salt stresses, proline contents, sugar contents, chlorophyll contents and relative water contents (RWC) were significa...
Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2
...itochondria). For stable transformation of B. napus floral-dip and tissue culture based approaches were tested using different formulations of phytohormones for calli, shoots and roots induction in a variety of genotypes. While false positive lines were obtained in the result of floral-dip method, the tissue culture based method led to successful regeneration of shoots on Murashige and Skoog (MS) media containing BAP 3 mgL-1 and developed roots in ½ MS ...
Betül Ayça Dönmez, Sarbesh Das Dangol and Allah Bakhsh*
...develop the protocol for transformation of Solanum chacoense diploid M6 potato using tetraploid S. tuberorsum cv. Desiree as a control using five different Agrobacterium strains harboring pBIN19 binary vector that further contains gusA gene, interrupted by an intronic sequence, under the control of 35S CaMV promoter. After transformation, we analyzed the transformation efficiencies of each...
Qaisar Jamal*, Akram Shah, Syed Basit Rasheed and Muhammad Adnan
...viding. During a 10 days transformation of axenic amastigotes, the day following inoculation to the day 3 different morphological forms were observed. The day 7 onward only rounded no flagellum (RNF=41-47%) and oval no flagellum (ONF= 53-59%) forms were present. Viability remained 96-98% during transformation. Bulk growth of the promastigotes for prolong (over a week) duration resulted in the acidification of medium and chan...
Anwar Ali1*, Muhammad Irfan Ashraf2, Saeed Gulzar2 and Muhammad Akmal2
...ined through logarithmic transformation of response and explanatory variables. It was found that square of DBH and H (D2H) was the best independent variable for predicting above ground biomass of an individual tree. The equation was also evaluated and compared with the existing generic allometric equations. Results showed that the allometric equation developed in this study gave more reliable estimates of aboveground biomass. A strong relationship (r2 = 0.99) ...
Muhammad Medrar Hussain*, Asad Jan* and Sayyar Khan Kazi
...h Agrobacterium mediated transformation, OsTZF8 gene (pBIG-Ubi-OsTZF8) was successfully transferred to rice calli. From these rice calli transgenic plants were regenerated and established in greenhouse. For the evaluation of OsTZF8 gene role in drought stress tolerance, two weeks old rice seedling were subjected to drought stress. OsTZF8-OX transgenic indica line A and B displayed 69% and 64% survival rates respectively compared to 33% of control. These result...
Farid Ullah Khan*, Tashfeen Ali*, Khubroo Jamil*
DEVELOPMENT OF A LOW VOLTAGE AC TO DC CONVERTER FOR MESO AND MICRO ENERGY HARVESTERS
...mplitude of 10 mV with a transformation factor of 6.5. The variation in the
input frequency had almost no effect on the DC output voltage of the converter. Upon integration of the converter
to different energy harvesters, the converter successfully rectified the AC or random output voltage signals of the
harvesters into corresponding DC voltage. ...
input frequency had almost no effect on the DC output voltage of the converter. Upon integration of the converter
to different energy harvesters, the converter successfully rectified the AC or random output voltage signals of the
harvesters into corresponding DC voltage. ...
Altaf Hussain Rajpar1, Abdul Hameed Memon1, Mohammad Akram Shaikh2, Weimin Zhang3
HAND-EYE COORDINATION FOR OBJECT GRASPING TASK OF HUMANOID ROBOT (BHR-02)
...;ii) hand-eye coordinate transformation iii) orientation of hand with target object iv) hand-eye localization. For hand localization and to limit the orientation problem a blue colored rectangle block was pasted on the robot hand. The blue colored rectangle block four sides were detected by applying karhunen loeve (K-L) transform, Hough transform and Least Square Error (LSE) methods. For hand-eye coordination a hand-eye model was developed ...
Zakeer Ahmed Khan Abbasi* and Allah Nawaz
...rable to the cataclysmic transformations in the climate system; causing productivity loss, soil degradation, water scarcity, resource depletion etc. Climate change awareness empowers the farming communities to take appropriate measures to mitigate the negative consequences of this phenomenon but the responses are not necessarily identical in scale and forms . In developed countries, climate change awareness is much advanced therefore, their climate change adap...
Sajid Khan* and Shahnaz Akhtar
Effect of Farm Labor Transformation on Households Income in Central Districts of Khyber Pakhtunkhwa, Pakistan
...the effect of farm labor transformation on households’ income in central three districts of Khyber-Pakhtunkhwa Province of Pakistan. Data on different aspects of farm labor transformation were collected through a well-designed and pre-tested questionnaire. Nine village councils from three districts were selected through random sampling techniques. The data were collected from 393 sample households and were analyzed by ...
Minghao Gong1*, Shiliang Pang2, Zhongyan Gao2, Wanyu Wen1, Ling Zhang3, Gang Liu1, Huixin Li1, Fawen Qian4 and Wenfeng Wang2
...n the study area and the transformation of wetland to farmland in the area be banned. Also, organic agriculture methods and crop species that consume less water than rice should be adopted to mitigate the adverse climate changes that accompany global warming and will contribute to RCC habitat degradation.
... 
Aya El-Turkey1, A. K. El-Attar1, A. E. Aboulata1, B. Othman2 and K. A. El-dougdoug2
... here, the use of stable transformation to develop transgenic tomatoes expressing the biologically active HSV-2 gD antigen (gD2). Thus, our results may have implications for the development of plant based vaccine production systems against HSV-2....
Song Jiang1,2,3, Xianbin Mo1,2, Falin Zhou2,3, Jianhua Huang2,3, Qibin Yang2,3, Lishi Yang2,3 and Shigui Jiang2,3*
...bility of survival after transformation was 0.38 and 0.22, respectively, which varied from medium to high heritability. The estimated heritability of body weight and transformed survival were 0.46 ± 0.10 and 0.32, respectively, which showed high heritability level. The estimates of genetic parameters might be overestimated because the common environmental effect was not included in the estimation model due to convergence problem. The results showed that...
Naveed Farah1*, Izhar Ahmad Khan1, Asif Ali Abro2, Jehanzeb Masood Cheema3 and Muhammad Luqman4*
...the resulting livelihood transformation of farmers at urban fringes in two contrasting cities of Punjab province of Pakistan. A mixed method approach was employed for data collection and at first, a longitudinal analysis of Landsat imagery of land use was done through GIS and Remote Sensing for a time period of 2001-2016. The results of GIS analysis revealed that during the period 2001 to 2016, Faisalabad showed 24% increase in urban area and 23 % of this expa...
Wafaa M.A. Ghoneem*, Muhammad M. Shaklouf, Ali M. Ali and Mohammed H. Bakr
...ntrol. While, lymphocyte transformation and phagocytic index were increased by (18 and 23%) and (20 and 62 %), respectively for G2 and G3 compared with G1. Average daily milk yield was increased by 2.23 kg/d and 0.9 kg/d in G2 and G3, respectively compared with G1. It could be concluded that addition of 10 g LAFP/h/d seems to be profitable whereas, it increased milk yield, improved feed efficiency and enhanced immune system response without significant effect ...
Mehwish Saleem Khan* and Sumama Farooq
...bes through conjugation, transformation and transduction. The present study was conducted at Ganga Ram Hospital Lahore in order to study the resistance pattern of gram-negative bacteria. Females are more prone to infections as compared to males due to anatomical, physiological, behavioral and hormonal imbalance that’s why majority of samples (33/60) were obtained from females as compared to males (27/60). From these collected samples exceeding numbers we...
Muhammad Shafiq1, Tehseen Ashraf2*, Sehrish Mushtaq1, Naveeda Anjum3, Muhammad Asim4, Muhammad Aqeel Feroze3, Malik Abdul Rehman4 and Marja Aziz3
...d cotyledon explants for transformation. The effects of co-cultivation at different temperatures (22 and 25oC), photoperiods (16 hours of light, 8 hours of darkness, and full darkness), and co-cultivation periods were studied. GFP assays revealed that the putative transgenic calli were not transformed and died after 40-60 days. The experiment was repeated ten times to see the success rate of the regeneration system. In this study a protocol for chili plant reg...
Xiajun Zhang1, Jie Yang2, Wenjun Zhou1, Zhenshi Chen2, Weidong Wu3, Shaoru Zhang2 and Lihui Wang2*
...f epithelial-mesenchymal transformation. Tumor cells activate the TGF-β/Smad pathway, and inhibiting its signal transduction pathway is an important strategy for tumor therapy. First, we have found lncRNA CASC7 is a poor prognostic marker of endometrial cancer. Then, ASPN was specifically expressed in endometrium and cell specific expression in basophils, among the most similar genes, we found TGF-β3. Moreover, lncRNA CASC7 acts on miR-26, and miR-26...
Neni Widaningsih1,2*, Budi Hartono2, Hari Dwi Utami2, Eni Siti Rohaeni3
...to examine the marketing transformation of swamp buffalo (Bubalus bubalis carabauesis) with digital-based marketing. This research has been carried out from January to June 2022 in Paminggir District, Hulu Sungai Regency, North of South Kalimantan Province. The methods used in this study are survey and observation methods. Data collection carried out includes; purposive sampling and accidental sampling techniques with the main respondents, namely breeders, col...
A.S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik 2; Nashwa M. A. Abd El-Mohsen1; and H. M. Abdelmaksoud1
...on and propagation after transformation process in E. coli. (2) Isolation of plasmid DNA. then used restriction enzymes Bam HI and Bgl II to identify clones containing inserts. To confirm the fragment inserted of CP gene sequence. PCR was performed using specific primers for PVYN CP gene. (3) The gene expression was performed using 100 µM IPTG & 2 µM biotin and incubation for 4h / 37 oC to produce biotinylated protein for E. coli with Mr 22.5 K...
Yajuan Zheng, Qiuping Mo, Hongchao Tang, Qinghui Zheng and Dandan Guan*
...increasing the malignant transformation rate of breast cancer....
Sidra Mumtaz1, Sidra Arshad1, Rizwan Ullah Khan1, Muhammad Usman Rashid2 and Naila Malkani1*
...bers of the erythroblast transformation specific (Ets) transcription factors family showing aberrant expression in various stages of tumor formation in breast tissue. However, their use as a prognostic factor is not very clear. Therefore, a meta-analysis was performed to analyze their aberrant expression in breast tumorigenesis. A thorough literature search was performed and 73 relevant studies were identified which were further scrutinized and finally 26 stud...
Mahfouz M.M. Abd-Elgawad
...ptimization and nematode transformation counts necessary to meet assumptions for parametric statistical analysis. Basically, they can help in unraveling the complexities of ecosystem dynamics into quantifiable metrics. Yet, their merits and demerits, discussed herein, should be considered by researchers and stakeholders. As they should be tailored to fit the intended goal of application, related modifications to derive more accurate and appropriate conclusions...
Adnan Ibrahim Khamis1, Jasim Mohammed Saleh2*, Najwa S. Ali3 and Anwer Thamer Ghaffoori2
...g stages of their social transformations, as they occupy an essential role in the process of development and economic and social development in all countries of the world, for their great role in employment. The study aims to know the role of small agricultural projects in developing the capabilities and competence of rural youth in Anbar Governorate through nine regions that represent the focus of capacity development. The research was conducted in Anbar prov...
Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3
...t cells using heat shock transformation method to get expression. The product size was confirmed by restriction digestion. The rOmpH protein was purified by immobilized metal affinity chromatography by using His-Ni resins to get desired protein and characterized qualitatively by SDS-PAGE by using His antibodies and western blotting. Once purified the immunogenicity of rOmpH was evaluated in BALB/c mice. The expression of recombinant OmpH protein (rOmpH) result...
Hala M. El-Makaky*•, Sherif, N.A.;Fekria El-Bordiny* and Taha, M.M.*
...lts of both T-lymphocyte transformation and ELISA tests revealed that the prepared vaccine induced good protection and has good potentiality for use in chicken in the Egyptian poultry industry....
Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4
...the matrix molecule. For transformation E. coli BL21 (DE3) strain was used. To assess the efficacy of the antigen, immunoassay analysis and blot-hybridization techniques were employed. Obtained results showed that 1mM isopropyl ß-D-1-thiogalactopyranoside induction at 2℃ for 18 hours was the most optimal condition for expression of recombinant GM6 T. evansi antigen. From a 500 ml of growth medium, a total of 3.4 mg of recombinant protein was successful...
