Bibi Tayyaba Batool1, Aemal Tareen1, Shahjahan Shabir Ahmed1, Hira Ejaz1, Mohammad Azam Kakar1, Syed Abdul Rehman2, Mohammed Saeed1, Zafar Ahmad3, Mohammad Masood Tariq3, Mohammad Arif Awan3 and Muhammad Naeem Shahwani1*
...">Brucellosis and bovine tuberculosis (TB) are a constraint to livestock production in Balochistan. The aim of this study was to determine the prevalence of brucellosis and bovine tuberculosis in cattle, goats and sheep in some primary dwelling districts of Balochistan. Our results showed that brucellosis was found 2.2% positive from Quetta district and 0.6% positive from Pishin district and bovine (TB) was found 0.60% posit...
Muhammad Yahya Noori1*, Saharish Rizwan2, Zaheer Ali3 and Shaheen Sharafat4
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
...own for a high burden of tuberculosis (TB). However, there is little information about the molecular characteristics of Mycobacterium tuberculosis (M. tuberculosis) strains predominant in the province. Therefore, this study was planned to study the genetic diversity in the M. tuberculosis isolates isolated from clinical samples. In the curr...
Niamatullah Kakar, Farhat Abbas, Muhammad Shafee and Tauseef Asmat*
... Mycobacterium tuberculosis is prerequisite for the successful treatment of TB. Aim of this study was to evaluate the prevalence of tuberculosis along with associated risk factors in Quetta Jail, Pakistan. In this cross-sectional study, 186 sputum samples were collected randomly from jail inmates. Sputum was processed for smear microscopy and culture inoculation for the detection of Mycobacterium
Masood Nizam Tabassum1*, Muhammad Ather Khan2, Saira Afzal3, Amir Gilani4, Abdul Wahab Gureja5, ShafaqTabassum6
...used by Mycobacterium tuberculosis (MTB). More than 95% of cases and deaths are reported in developing countries. Tuberculosis affects all age groups but adults are mostly affected in their most productive age. However, all age groups are at risks. In the world, TB is one of the major killer infectious diseases, which place it in one of the top 5 causes of death for females aged 15 to 44. The death rate due to this is...
Muhammad Kashif Munir1*, Sana Rehman1, Rizwan Iqbal1, Muhammad Saqib Saeed2, Muhammad Aasim1
... and multidrug resistant tuberculosis is an emerging global issue. Rapid detection of such type of tuberculosis is necessary for timely control of the disease. GeneXpert test has already been implemented by World Health Organization to diagnose the infection on urgent basis.
Objectives: This study was designed to apply GeneXpert MTB/RIF assays for the detection of rifampicin resistant
Asad Ullah1, Umar Sadique Khattak2, Sultan Ayaz1, Muhammad Subhan Qureshi2, Imad Khan1, Ibad Ullah Jan2, Irfan Khattak1, Raheela Taj3, Sahar Nigar4, Naimat Ullah Khan1,Mumtaz Ali Khan5 and Muhammad Luqman Sohail6,*
...d risk factors of bovine tuberculosis (bTB) among large ruminants of the five districts (Peshawar, Nowshera, Charsadda, Mardan and Swabi) located in the central zone of Khyber Pakhtunkhwa (KPK), Pakistan. A total of 2400 large ruminants were sampled by convenience sampling approach and tested by comparative cervical intradermal tuberculin test (CCIT) for detection of Mycobacterium bovis infection. Data regarding socio-demographic status, risk factors an...
Sobia Faisal1*,Qurat-ul-Ain Ahmad2, Maleeha Manzoor3, Madiha Manzoor4, Faisal Rifaq5
Socio-psychological factors related to tuberculosis diagnostic test seeking behavior in Pakistan
...alth seeking behavior of tuberculosis (TB) patients globally,
however, the need to understand why these factors affect the health seeking
behavior of TB patients in the light of behavioral theories in still unmet. This study
evaluates the role of social and psychological factors affecting TB diagnostic test
seeking behavior of patients in low socioeconomic country like Pakistan in relation
to “Health Belief Model”...
however, the need to understand why these factors affect the health seeking
behavior of TB patients in the light of behavioral theories in still unmet. This study
evaluates the role of social and psychological factors affecting TB diagnostic test
seeking behavior of patients in low socioeconomic country like Pakistan in relation
to “Health Belief Model”...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
...es.
...
Ambreen Leghari1,2, Asghar Ali Kamboh2,*, Shakeel Ahmed Lakho1, Faiz Muhammad Khand1, Kanwar Kumar Malhi2,3, Iqra Bano Chandio4, Seema Baloch5 and Jan Mohammad Shah4
...the prevalence of bovine tuberculosis (BTB) in randomly selected cattle (n= 160) was carried out in Hyderabad and Tando Allahyar districts. The animals were first screened through single intradermal tuberculin test (SITT) and both positive and negative reactors of SITT were further investigated through rapid test. Attempts were also made to isolate the Mycobacterium bovis organism from the milk and nasal secretions of cattle using the Lowenstein-Jensen ...
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*
...1A1 methylation and anti-tuberculosis drug-induced liver injury are available. Studying the relationship between CpG island methylation in the CYP1A1 promoter region and drug-induced liver injury is, therefore, of great importance. Anti-tuberculosis drugs induced hepatotoxicity in HL-7702 cells was investigated. Dehydrogenase activity was detected by a lactate dehydrogenase assay kit; superoxide dismutase (SOD) activity and ...
Asad Ullah1*, Umar Sadique2, Sultan Ayaz1, Muhammad Subhan Qureshi2 and Farhan Anwar Khan2
...the prevalence of bovine tuberculosis (bTB); detection of Mycobacterium bovis (M. bovis) infection in lactating indigenous cattle/buffaloes raised on different commercial dairy farms and exposing the hazards linked with milk-born tuberculosis infection in the area where unpasteurized milk is normally used in the central zone of Khyber Pakhtunkhwa (KP), Pakistan. A total of 1225 cattle and 1175 buffaloes of more than one year...
Yuhong Li1, Dongxue Wu1, Xue Wang1, Mi Zhang1, Hanyu Zhu1, Zhe Shi1 and Fumin Feng1,2*
...r injury induced by anti-tuberculosis drugs in mice. A total of 168 Kunming mice were randomly divided into four experimental groups and one baseline group. The drug groups were orally administered with isoniazid (H), rifampicin (R), pyrazinamide (Z), and combination of these three drugs (HRZ) at 3, 5, 7, 10, and 15 days, respectively. Liver tissue pathologies and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities changed, indicatin...
Muhammad Zahid Mengal1, Hamida Ali2, Raheela Asmat3, Muhammad Naeem1,4, Ferhat Abbas1, Abdul Samad1, Mohammad Zahid Mustafa1, Jannat Raza2 and Tauseef M. Asmat1*
...F has revolutionized the tuberculosis diagnosis by simultaneous detection of Mycobacterium tuberculosis and resistance to RIF (rifampicin), a surrogate marker for multidrug-resistant TB in less than two hours. The RIF-resistance pattern in Balochistan, Pakistan, is not documented. This study was aimed to detect RIF-resistant TB and mutations in RNA polymerase beta (rpoB) gene of M. t...
Eman Khalifa1*, Riad Khalil2 and Haitham Elaadli3
... highlight on dangers of tuberculosis as a zoonotic and debilitating disease threatening animal and human health beside economy, this research aimed to determine the prevalence of Mycobacterium bovis from buffaloes’ mastitic milk by isolation on Lowenstein-Jensen medium (LJ medium), Ziehl-Neelsen staining; identification by different biochemical tests and polymerase chain reaction (PCR) at 500bp diagnostic for M. bovis, as well as (2) to de...
Samina Tahir Kiani1, Abdul Rauf1, Syed Ayaz Kazmi1,*, Nuzhat Shafi1, Madiha Khalid2, Naeem Latif1, Samina Gul2, Saghir Yousaf3 and Muhammad Arshad4
Muhammad Anwarullah1, Aneela Zameer Durrani1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Usman1*,Muhammad Zahid Iqbal1, Muhammad Husnain1,Ahsan Anjum3, Qurratulain Ashraf4 and Ghulam Mustafa3
...avium sub sp. paratuberculosis (MAP) in cattle and buffalo. Diarrhea is a major risk factor for MAP, so 1100 blood samples were collected from diarrheic animals or having history of some diarrheic episodes from four districts of Punjab i.e. Kasur (n=300), Rawalpindi (n=230), Lahore (n=230) and Bahawalpur (n=340). These samples were tested using ELISA and overall seroprevalence found was 39.64%. The major host-associated and non-associated risk facto...
Asad Ullah1*, Umar Sadique2, Ibadullah Jan2, Imad Khan1, Raheela Taj3, Mumtaz Ali Khan4, Salah-ud-din4, Naimat Ullah Khan1
...iggered by Mycobacterium tuberculosis (MTB). Bovine tuberculosis (bTB) is one amongst neglected zoonotic disease (NZD) caused by Mycobacterium bovis; has an economic importance with public health significance and life-threatening infection at the human-animal-ecosystem interface in low-resource settings and developing countries globally. Our study aimed to investigate the incidence of pulmonic tuberc...
Muhammad Qasim Mazari1, Dildar Hussain Kalhoro1,*, Hasina Baloch1, Muhammad Saleem Kalhoro2, Shahid Hussain Abro1, Rehana Buriro1, Asmatullah Kaka1, Fahmida Parveen1, Mazhar Hussain Mangi3, Ghulam Murtaza Lochi1, Abdul Ahad Soomro4, Ali Gul Soomro1, Abdul Ghaffar Abbasi1 and Sarfraz Hussain Depar1
...ly. Prevalence of bovine tuberculosis in Mirpurkhas district via cultural examination was recorded as 1.66%, 2.5% in milk and nasal samples, while in district Badin it was 1.66%, 5%, respectively. The overall prevalence of bovine tuberculosis was 5.5% and 7% in Mirpurkhas and Badin districts through tuberculin, Rapid BTB Ab, Culture examination and ELISA test. Different tests such as SITT, Rapid BTB Ab, culture examination a...
Muhammad Idrees1, Bashir Ahmad2, Muhammad Waqas1, Syed Muhammad Mukarram Shah3 and Saad Ahmad Khan4
...d to discover novel anti-tuberculosis agents with novel modes of action. In this study, small molecule inhibitors of the proteins encoded by the drug resistant genes, i.e., katG, gyrA, pncA and rpoB of Mycobacterium tuberculosis (M. tuberculosis), were identified using computational methods. In the ligand base pharmacophore, an already reported four ligands for the four proteins encoded by...
Ouedraogo Oumar1,2, Tianhoun Denté Fidèle2,4*, Séré Modou3, Kaboré Adama2, Tamboura H. Hamidou2 and Belem Adrien Marie Gaston4
...omic incidence of bovine tuberculosis. For this purpose, daily monitoring of cattle from various farms and slaughtered at the slaughter area was carried out from February to June 2019 during ante and post-mortem inspections. The results of the analysis of the data collected during these inspections revealed that 1,716 cattle of local breeds, consisting of 875 females and 841 males, were slaughtered and checked by the veterinary inspection officers. At post-mor...
Saleha Tabassum1, Muneeza Zafar1,2, Munazza Raza Mirza1*, Muhammad Iqbal Choudhary1,3, Ikram ul Haq4, Imran Riaz Malik2, Ambreen Ansari1 and Mehtab Alam5
...ants, including 122 with tuberculosis disease (TB) and 138 healthy control (HC) were included. Out of these 122 TB disease subjects (67%) were diagnosed with PTB (n = 82) and 33% with EPTB (n = 40). Tri and Tetra- ARMS-PCR techniques were used to genotype rs2276886 of CXCL9 and rs5606198 of CXCL10 for all the samples. The results showed significant association (OR, 2.1 (0.99 to 4.30), p = 0.04) of A allele of rs2276886 with EPTB group as compared to the contro...
Muhammad Izhar ul Haque1, Farhan Anwar Khan1*, Umar Sadique1, Hamayun Khan1, Zia ur Rehman1, Salman Khan1, Hayatullah Khan1,2, Faisal Ahmad1,3, Mumtazur Rahman1, Faiz Ur Rehman1, Muhammad Saeed1, Mehboob Ali1 and Saqib Nawaz1
...ext-align: justify;">Paratuberculosis (pTB) also known as Johne’s disease (JD), is a chronic infectious disease of animals caused by Mycobacterium avium subsp. paratuberculosis (MAP). It is also a serious public health concern as MAP is responsible for Crohn’s disease in human beings. This infectious disease is so far unexplored in animals in Khyber Pakhtunkhwa (KP) province of Pakistan. Therefore, this study was...
Ayma Aftab, Samia Afzal* and Muhammad Idrees
...ntation rate (ESR) test, tuberculosis test, real time PCR and high-resolution computed tomography (HRCT) were done to rule out the cause of flu like symptoms. HRCT reveals a haze area in the right perihilar region adjacent to medial part of horizontal fissure on the 3rd day of manifestation of symptoms. Radiological studies showed early consolidation of COVID-19 whereas RT-PCR showed negative results. Chest CT imaging is a highly sensitive technique that has a...
Rafik Soliman1, Neven Waheeb2, Essam Nasr2, Mahmoud El-Hariri1, Heidy Abo-Elyazeed1, Hassan Aboul-Ella1*
...-align: justify;">Bovine tuberculosis is a worldwide disease that causes great economic losses to the dairy industry and constitutes a serious human public health hazard. Development of a wide range of serodiagnostic assays for mycobacterium has been an area of interest through the past two decades as a rapid, accurate, and easy-to-be-used and interpreted diagnostic kit as well as to be a competitive alternative to the conventionally established expert-depende...
Meng Wang, Dongliang Zhou, Huixia Li, Chunqin Wu,Yan Zhao and Houqiang Luo*
...s found in Mycobacterium tuberculosis. T5SS may be the simplest secretion system available among all the secretion systems, and plays a vital role in the pathogenic mechanism of bacteria by participating in bacterial adhesion, biofilm formation, and contributing to the ability of nutrition acquisition a nd environme ntal adaptability. T5SS is a vital factor in the gradual reduction of antibiotic effectiveness, so the system has been the target of alternative a...
Martin-Luther Oseni Okolo1, Monday Eneojo Akor1, Cornelius Arome Omatola1*, Zainab Penninah Ochada1, Ugbane Eleojo Okolo2, Benjamin Mudi Idache3, Andrew Isiramen4, Julius Akor Omatola5 and Moses Adaji David6
...ign: justify;">Worldwide tuberculosis (TB) prevalence increased as a result of the Human Immunodeficiency Virus (HIV) epidemic. Nevertheless, the widespread use of efficient antiretrovirals has lately prompted the tendency to reverse. This study was carried out to detect Mycobacterium tuberculosis using GeneXpert in HIV seropositive patients attending Kogi State Specialist Hospital, Lokoja. The study was done using 325 confi...
Mohammed Naji Odhah1,2, Faez Firdaus Abdullah Jesse1*, Bura Thlama Paul3,4, Bashiru Garba5, Zaid Mahmood1, Eric Lim Teik Chung6, Mohd Azmi Mohd Lila7
...
Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis in small ruminants and is of considerable economic importance in many countries worldwide. The control of the disease in animals depends on the control of the disease in infected animals. Nevertheless, few countries where the disease was previously endemic have successfully eradicated the pathogen. This investigation was undertaken to determine the antibody titre (IgM and IgG...
Razia Sultana1, Shinawar Waseem Ali1*, Ghulam Murtaza2 and Shahid Mahmood2
...e species (Mycobacterium tuberculosis, Pantoea agglomerans, and Raoultella ornithinolytica) belonged to culturable bacteria and one species (Tepidimonas spp.) belonged to nonculturable bacteria. Our data demonstrate the presence of wide diversity of bacterial microbiota in locally processed back-slopped yogurt. ...
Muhammad Ashfaq ur Rahman1*, Saleem Khan1, Aurang Zeb1, Zia ud Din1 and Zafar Iqbal3
...>Patients with pulmonary tuberculosis (pTB) often suffer from malnutrition, hence this issue has to be taken into account while designing a treatment plan. Patients with pulmonary tuberculosis (pTB) seeking treatment at the TB Centre in Baghdada, Pakistan, were surveyed to determine their body weight before and after receiving dietary counseling. A total of 150 patients were enlisted for this prospective analytic case-contro...
Sarah G. Yousef1*, Ahmed Shehta2, Hend M. El Damaty1 and Hussein A. Elsheikh3
...cterium avium subsp. paratuberculosis (MAP), is regarded as a potential public health issue and a significant threat to the dairy and agricultural food businesses. This study was conducted for the molecular investigation of MAP in cattle reared for small-scale dairy production in Sharkia Governorate, Egypt. Seventy-five fecal samples were collected from diarrheic and healthy dairy cows that came into contact with diarrheic ones for molecular screening. Clinica...
Asad Ullah1, Hayat Ullah2, Muhammad Lateef3, Imrana Niaz Sultan4, Afrasiab Khan Tareen4 and Muhammad Waseem Khan4,5*
Kouengoua Kouengoua Armelle Prudence1*, Yessinou Roland2, Nankam Chimi Roland1, Fotsac Dzousse Muller1, Kouam Alain1, Djuikwo Felicite1, Facho Balaam1, Awah Ndukum Julius3, Farougou Souaibou2
... />
...
Kashif Abdaal, Aneeza Batool, Muhammad Tariq Navid*, Saeed Ahmed, Asma Saleem Qazi, Waseem Safdar, Haidar Ali, Mobeen Ur Rashid, Somia Rafaqat
...ng ones like malaria and tuberculosis and exploring their potential applications in non-infectious conditions like Alzheimer’s. Traditional vaccines such as: live attenuated and inactivated have long been treated as effective against infectious diseases. However, recent advancements in vaccine development strategies including mRNA- and recombinant DNA technologies along with viral vector-based vaccines containing specific DNA segment(s) of an infectious ...
María Julia Traversa1*, Mónica Saracco2, María Alejandra Colombatti Olivieri3, Fernando Paolicchi4, Edgardo Rodriguez5, Silvia Estein5, María Cristina Jorge5, William C. Davis6
...arms with endemic bovine tuberculosis (TB) and nonreactors from a dairy farm free of PPDb reactors for 12 years. In PPDb reactors, CD14 expressing cells increased significantly after specific stimulation. In PPDbEPPp group, B B2 cells expanded significantly (p=0.004), and cells with the activation marker CD25 (or interleukin-2 receptor α chain) expanded significantly (p= 0.03) with half IFNγ production. Results from this study suggest that in natur...
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