Age-Dependent Changes in Muscle Regulatory Factors and Glycogen in Broiler Chickens: A Histological and Molecular Study
Age-Dependent Changes in Muscle Regulatory Factors and Glycogen in Broiler Chickens: A Histological and Molecular Study
Nabeel Abd Murad Al-Mamoori*, Haneen Abdul Ameer Abbas**
Representative histological sections of the iliotibialis muscle from broiler chickens at different ages (7, 21, and 38 days), stained with Hematoxylin and Eosin (H&E), Masson’s Trichrome, and Periodic Acid–Schiff (PAS) to reveal muscle fiber morphology, connective tissue, and glycogen content. Indicators: (a) Epimysium, (b) Perimysium, (c) Endomysium, (d) Nucleus, (e) Muscle fiber, (f) Glycogen. Magnification 400X.
Represented the muscle fiber area in the Iliotibialis muscle at 7, 21, and 38 days of age of the broiler chickens. Data is expressed as mean± standard error (n = 5). * p<0.05.
Represented the muscle bundle area in the Iliotibialis muscle at 7, 21, and 38 days of age of the broiler chickens. Data is expressed as mean± standard error (n = 5). * p<0.05.
Represented the thickness of connective tissue in the Iliotibialis muscle at 7, 21, and 38 days of age of the broiler chickens. Data is expressed as mean± standard error (n = 5). * p<0.05.
Quantification of the MYH7, MYOG, and MYF5 transcripts in the Iliotibialis muscle in broiler chicken using RT-qPCR. The expression of MYH7, MYOG, and MYF5 genes was normalized to the expression of GAPDH and is presented as gene expression ratios. Each column denotes the gene and age of the broiler chickens. Data is expressed as mean± standard error (n = 5). * p<0.05.
qPCR plots of the MYOG gene in the Iliotibialis muscle. The green, blue, and red plots are for 7, 21, and 38 days of age, respectively.
qPCR melt peak of the MYOG gene in the Iliotibialis muscle. The green, blue, and red plots are for 7, 21, and 38 days of age, respectively.
qPCR plots of the MYF5 gene in the Iliotibialis muscle. The green, blue, and red plots are for 7, 21, and 38 days of age, respectively.
qPCR melt peak of the MYF5 gene in the Iliotibialis muscle. The green, blue, and red plots are for 7, 21, and 38 days of age, respectively.
qPCR plots of the MYH7 gene in the Iliotibialis muscle. The green, blue, and red plots are for 7, 21, and 38 days of age, respectively.
qPCR melt peak of the MYH7 gene in the Iliotibialis muscle. The green, blue, and red plots are for 7, 21, and 38 days of age, respectively.
qPCR plots of the GAPDH gene in the Iliotibialis muscle. The green, blue, and red plots are for 7, 21, and 38 days of age, respectively.
qPCR melt peak of the GAPDH gene in Iliotibialis muscle. The green, blue, and red plots are for 7 , 21, and 38 days of age respectivaly.
