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Assessing Haemato-Biochemical, and Genotoxic Effects of Pesticide Exposure on Rural Inhabitants in South Punjab, Pakistan: A Biomonitoring Study

Assessing Haemato-Biochemical, and Genotoxic Effects of Pesticide Exposure on Rural Inhabitants in South Punjab, Pakistan: A Biomonitoring Study

Abdul Ghaffar1*, Ayesha Maqsood1, Riaz Hussain2, Ghulam Abbas3, Rabia Tahir4, Habiba Jamil1, Fozia Afzal5, Ahrar Khan6,7, Muhammad Ahmad Chishti1, Shahnaz Rashid3, Aliya Noreen8, Kashfa Akram1 and Maria Niaz1

¹Department of Zoology, The Islamia University of Bahawalpur, 63100, Pakistan; 2Department of Pathology, University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur-63100, Pakistan; 3Centre of Excellence in Marine Biology University of Karachi, 75270, Pakistan; 4College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China; 5Department of Zoology, The Islamia University of Bahawalpur, Rahim Yar Khan Campus, 6400, Pakistan; 6Shandong Vocational Animal Science and Veterinary College, Weifang, 261061, China; 7Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan; 8Biology Centre CAS, Institute of Entomology, Ceske Budějovice, Czechia.

 
*Correspondence | Abdul Ghaffar, Department of Zoology, The Islamia University of Bahawalpur, 63100, Pakistan; Email: dr.abdul.ghaffar@iub.edu.pk 

ABSTRACT

Blood samples (n=100) were collected from the people in district Bahawalnagar, Punjab who were exposed to pesticides, a total of 5ml blood was collected in both EDTA tubes and serum vials. The purpose was to study the hematological, biochemical, and genotoxic potential in rural inhabitants exposed to pesticides. Samples were collected across different age groups and compared with those from 100 unexposed individuals. Demographic characteristics of the pesticide-exposed workers in the district were also observed. Hematological and biochemical parameters, including WBCs, RBCs, hemoglobin, HCT, MCHC, MCH, lymphocytes, MCV, neutrophils, monocytes, eosinophils, potassium, sodium, calcium, and phosphorus, as well as AST, CPK, LDH, glucose, urea, creatinine, bilirubin, ALT, and ALP, were analyzed. Multiple comparisons were made with different diseases, revealing variations between pesticide-exposed and unexposed individuals. The comet assay method was utilized to observe damaged DNA in pesticide-exposed individuals and compare its prevalence across different age groups. The findings indicated an increase in the rate of DNA damage with age. Highly damaged cells exhibited a greater tail length of DNA compared to slightly damaged, damaged, and undamaged cells.

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Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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