Characterization of Alkalophilic Detergent Compatible Amylase from Bacillus halodurans, Isolated from a Restaurant’s Washing Area
Characterization of Alkalophilic Detergent Compatible Amylase from Bacillus halodurans, Isolated from a Restaurant’s Washing Area
Sana Safdar and Javed Iqbal Qazi*
ABSTRACT
Bacillus halodurans (NR_025446.1) amylase producing bacterium has been isolated from a restaurant washing influenced soil. The alkaline amylase produced by the Bacillus halodurans (NR_025446.1) has been characterized for its compatibility as detergent additive. The yield of alkaline amylase was found optimum after 48 h of incubation (10.97 U/ml) showing pH optima at 10 and temperature optima at 37°C.The amylolytic activity was enhanced by non ionic detergent components; Triton X-100, Tween-80 and moderately decreased in presence of SDS and oxidizing agents such as H2O2 and NaClO. The enzyme showed outstanding stability and compatibility with some commercially available laundry detergents. The enzyme maintained more than 85% of its initial activity after being incubated with 7 mg/ml of Sufi, Express and Surf excel locally available commercial detergents at 30◦C for the incubation time of 60 min. The addition of the alkaline amylase to the Sufi brand detergent amended its starch-based stain removal. The detergent compatible bacterium and its amylase appear promising for bio-detergent applications.
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