Detection and genetic analysis of bovine ephemeral fever virus G gene in buffy coat samples from cattle at Qualyubia, Egypt 2017
Detection and genetic analysis of bovine ephemeral fever virus G gene in buffy coat samples from cattle at Qualyubia, Egypt 2017
Ayman S. El-Habbaa1 and Mervat E. Radwan2
ABSTRACT
Background: Bovine Ephemeral Fever (BEF) is endemic in Egypt with repeated periodic outbreaks.
Objective: To investigate strain detection and genetic analysis of BEF virus among suspected cattle in
August 2017, Qualyubia, Egypt.
Methods: Partial sequence was generated after detection by reverse transcription-polymerase chain
reaction (RT-PCR) and subsequent gel purification of the amplified products of G gene of BEF virus.
Results: BEF virus was circulating in this region. Sequence analysis of G-gene of this Egyptian strain,
comparing with sequences of BEF virus circulating globally and regained from GenBank, showed
100% nucleotide homology with BEF virus from Egypt 2014 but nucleotide and deduced amino acid
substitution generates were showed with other BEF viruses that reduced this homology. Phylogeny
showed that BEF viruses from Egypt 2014 and 2017 had close homology to BEF virus circulated in
Israel during the same period suggesting that the virus was circulated in middle east.
Conclusion: These findings demonstrated the recent picture of BEF virus which incriminated for cattle
infectivity and responsible for its persistence in the endemic areas. Such epidemiological data could
guide the application of efficient control strategies of BEF virus in Egypt.
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January 2019
Vol. 5, Pages 1-141
