Microvariant coxI Gene of Echinococcus granulosus Sensu Stricto Cysts Found in One Sheep Liver with Two Million of Protoscoleces
Microvariant coxI Gene of Echinococcus granulosus Sensu Stricto Cysts Found in One Sheep Liver with Two Million of Protoscoleces
Gang Guo1,2, Wenjing Qi1,3, Baoping Guo1, Tian Wang4 and Jun Li1*
ABSTRACT
Echinococcus granulosus (Batsch, 1786) is a zoonotic agent and sheep is an important intermediate host in the lifecycle of cystic echinococcosis (CE). Above 2 million of protoscoleces were collected and counted by means of microscopic examination from one sheep liver which was derived from Urumqi, Xinjiang. To determine the genotypes of the parasite, DNA was extracted from germinal layers which had been washed with 1×PBS, isolated randomly from 12 independent hydatid cysts in the sheep liver respectively and used as templates to amplify the partial nucleotide sequences from mitochondrial NADH dehydrogenase 5 (nad5) and cytochrome coxidase subunit I (coxI) by polymerase chain reaction (PCR) assay. The PCR products were sequenced and analyzed further and G1 genotype of E. granulosus sensu stricto (s. s.) was confirmed. Furthermore, two microvariants of coxI gene were exhibited among 12 cysts. Our finding indicates that the biological capacity of the host for harboring E. granulosus s. s. is huge and likely that impacts the transmission of CE that may result in difficulty in terms of the control of CE to some extent. By genotype analysis, it suggests that G1 genotype of E. granulosus s. s. with different genetic microvariants can exit in a single sheep liver and may be responsible for the complexcity of vaccination developing, diagnostic tests and drug therapy on CE.
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