Proteomic Analysis by Shotgun LC-MS/MS of Cyst Wall Protein Components From the Protozoan Parasite Cryptocaryon irritans, the Causative Agent of White Spot Disease in Marine Fish
Proteomic Analysis by Shotgun LC-MS/MS of Cyst Wall Protein Components From the Protozoan Parasite Cryptocaryon irritans, the Causative Agent of White Spot Disease in Marine Fish
Honghua Wang1,2, Summia Perveen1,2, Shucheng Shao1,2, Kun Wang1,2 and Fei Yin1,2,*
ABSTRACT
To illustrate the protein composition of the Cryptocaryon irritans cyst wall, a shotgun-liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was applied and all identified proteins were categorised using a bioinformatics approach. The results showed that 3,472 proteins were identified with a theoretical molecular weight of 11.53–610.90 kDa and an isoelectric point of 4.13–11.77. A total of 63.07% or 50.09% proteins were annotated by Kyoto Encyclopaedia of Genes and Genomes (KEGG) or Gene Ontology (GO) terms, respectively. These results highlight that mass material is transported and catabolised and growth- and death-related proteins are highly expressed, including a number of vesicle-mediated transport, cytoskeletal and extracellular secretion-related proteins. These results provide a platform to study tomont cell characteristics and provide a further understanding of cyst wall formation and mechanisms.
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April 2020
Vol. 52, Iss. 2, Pages 425-824
