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Genetic Characterization of Methicillin Resistant Staphylococcus pseudintermedius in Dogs and Cats in Cyprus: Comparison of MRSP and MRSA Results

Genetic Characterization of Methicillin Resistant Staphylococcus pseudintermedius in Dogs and Cats in Cyprus: Comparison of MRSP and MRSA Results

Hazel Tamakan* and Huban Gocmen

Department of Microbiology, Faculty of Veterinary Medicine, Near East University, Nicosia, 99138, Northern Cyprus, Mersin-10, Turkey.

 
* Corresponding author: hazel.tamakan@neu.edu.tr

ABSTRACT

The aim of this research was to investigate the existence and frequency of Staphylococcus pseudintermedius in cats and dogs with pyoderma and otitis externa by conventional culture methods, automated identification system and PCR-RFLP. This is the first study to research the presence of MRSP in cats and dogs in Cyprus. The PCR-RFLP method based on the pta gene was confirmed to S. pseudintermedius. Methicillin resistance results were revealed with VITEK 2 and mecA gene detection was performed by PCR. A total of 100 samples including 50 cats and 50 dogs were examined from 6 veterinary clinics. S. pseudintermedius (4%) and S. aureus (8%) were isolated in cat samples; S. pseudintermedius (56%) S. aureus (6%) and S. schleiferi (2%) were isolated in dog samples, and 3 MRSP strains were identified. Methicillin resistance of these isolates was found to be statistically significant (χ²= 90.013, p<0.0001). As in MRSA, the increasing presence of both S. pseudintermedius and methicillin resistance has started to become a global problem, and with this study, awareness will be raised in both patient owners and veterinary clinics so that they can take the necessary precautions.

 

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Pakistan Journal of Zoology

December

Vol. 54, Iss. 6, Pages 2501-3000

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