Muhammad Zubair, Sajid Mahmood Sajid
...tion. Clomifene inhibits estrogen receptors in the hypothalamus, inhibiting negative feedback of estrogen on gonadotropin release and leading to up-regulation of ovulation. It has useful effects on the reproduction of female in terms of reduction in puberty and ovulation induction. The beneficial effects are manifested in male in terms of improvement of semen....
Mehwish Faheem1,*, Saba Khaliq2 and Khalid Parvez Lone2
... used as a biomarker for estrogenic endocrine disruption. To elucidate the endocrine-disrupting effect of bisphenol-A, juvenile Catla catla was exposed to graded concentrations of bisphenol-A (10,100,1000μg/l) for 14 days. BPA exposure strongly elevates vtg mRNA level in fish exposed to 100μg/l but at 1000 μg/l exposure of BPA, vtg level decreased compared to 100µg/l exposed fish. These results showed that BPA has
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*
...affect the production of estrogen. In the present study, to detect the effects of letrozole on gonad differentiation, 5dph fry of carp were exposed to different concentrations of LET. At 130 dph, sex ratio was recorded. The results showed that continuous exposure to LET induced the changes of sex ratio. High concentration of LET can promote male-biased sex ratio. In order to reveal the molecular mechanism by which LET caused sex reversal, juvenile carp (120dph...
Sadaf Ghulam Rasul1*, Uzma Yaqub2, Maida Manzoor1 and Hafsa Mubasshar1
...fy;">Endometriosis is an estrogen-regulated disease, and is signified by the existence of a particular endometrial tissue exterior to the uterus. It is a principal cause of long lasting morbidity, mostly from serious pelvic pain(1). Endometriotic tissue has the potential for aromatase gene expression that leads to aromatase and estrogen production. Danazol is a synthetic androgen whereas Letrozole is aromatase inhibitor. Thi...
Zartasha Anjum1*, Rakhshindah Bajwa2 and Munazza Hassan3
... along with tamoxifen in estrogen receptor (ER) and HMG-COAR positive breast carcinomas. However, in our local population, expression of HMG-COAR is not investigated. The objective of our study was to examine the expression of HMG-CoAR in primary breast carcinomas and determine its association with ER/PR expression.
Material & Methods: Cross-sectional analytical study was conducted to examine the immunohistochemical expression of HMG-CoA...
Amna Younus1,3, Mariam Faiz2 and Abida Yasmeen1,*
...II tumor type (47%), -ve estrogen receptor/ progesterone receptor (-ER/PR: 35%) and negative human epidermal growth factor receptor 2 (-ve Her2: 53%) hormonal status. Results also depicted that FokI and TaqI genotypic frequencies were significantly (P<0.001) distributed among women with different tumor grades and ER/PR status. FokI genotypic distribution was significantly associated (P<0.001) with different Her2 status whereas Taq...
Jawaria Shaheen1, Samiah Shahid1, Sabahat Shahzadi2, M. Waheed Akhtar1,3 and Saima Sadaf1,*
...cancer due to absence of estrogen, progesterone and HER2 receptors. We aimed to identify a panel of circulating miRNAs which can act as diagnostic and prognostic biomarkers specifically for TNBC. The blood samples were collected from 37 TNBC diagnosed patients and 34 age-matched non-TNBC controls to investigate the expression of miRNA panel. Plasma was isolated by standard protocol of centrifugation and then isolation of total RNA including miRNA was done. Abo...
Jun Yan Bai*, Zhi Hao Dong, Hui Rong Gong, Xiao Ning Lu and Zi Heng Li
...nvestigate the effect of estrogen receptor gene (ESR1) polymorphism on the carcass traits of laying quail. The polymorphism of ESR1 gene exon 4 in three laying quail populations was detected by PCR product sequencing, and the correlation between ESR1 gene and carcass traits of laying quail was analyzed. The results show that, Exon 4 of ESR1 gene among Beijing white quail, the pectora weight and pectoral muscle rate of CC genotype was significantly higher than ...
Bingjie Zhou1, Hitesh Bhagavanbhai Mangukiya1, Siva Bharath Merugu1, Fakhar-un-Nisa Yunus1, Yuchen Fan1, Zhenghua Wu1,* and Dawei Li1,2,*
...itially discovered as an estrogen-responsive gene in breast cancer cell lines, is a developmentally regulated gene belonging to the protein disulfide isomerase (PDI) gene family. AGR2 exists in both intracellular and secreted form, it is overexpressed in many types of cancer cells and also detected in extracellular space of solid tumor interstitial fluids. The intracellular AGR2 is critical in protein folding while secreted AGR2 function as a paracrine signal ...
Jun Yan Bai*, Yu Chen, Qi Hang Hu, Zhi Hao Dong, Ying Lei, You Bing Yang, Shu Juan Zhao and You Zhi Pang
...nvestigate the effect of estrogen receptor gene (ESR) polymorphism on the growth traits of quails. PCR products were used to directly sequence the polymorphism of exon 4 of ESR gene in three quail populations (Chinese yellow quail, Korean quail, Beijing white quail) and analyze the association between ESR gene and growth traits of quails. The results show that three genotypes, CC, CT and TT, were detected in exon 4 of ESR1 gene in t...
Zhi Hao Dong1, Jun Yan Bai1*, Xiao Ping Jia2, Ying Lei1, Hui Cao1, You Bing Yang1 and Guang Lu Li1
... the correlation between estrogen receptor (ESR) gene polymorphism and growth traits in two populations of quail. The results showed that three genotypes, CC, CT and TT, were detected in exon 1, exon 4 and exon 8 of ESR gene. The frequencies of CC genotype in expressed exon 1 of ESR gene were the highest in french giant quail and savimit quail (0.531 and 0.778, respectively). The frequencies of TT genotype in expressed exon 4 of ESR...
Arman Sayuti1, Hamdan Hamdan2, Miftahul Jannah3, Safriadi Safriadi3, Teuku Nasri3, Tongku Nizwan Siregar2*
...ncentration of steroids (estrogen and progesterone) in the local rabbits. In this study, we used 10 non pregnant rabbits that have given birth and were healthy clinically. All rabbits were randomly divided into two treatment groups, namely control group (K1) and treatment group (K2) where each group consist of five rabbits. The rabbits of K1 group were injected with aquabides for three consecutive days at a doses of 1.0, 0.5 and 0.3 mL each, whereas rabbits K2...
Mohamed Abd El-Fattah Abo-Farw1, Maged Ahmed Aboul-Omran1, Abdel-Khalek El-Sayed Abdel-Khalek2*
...on of progesterone (P4), estrogen (E2), thyroxin (T4), zinc (Zn), and selenium (Se) by about 24.05, 13.80, 5.36, 2.50, and 3.44%, respectively, while triiodothyronine (T3) concentration showed non-significant decrease by about 3.77%. Mastitis incidence at early postpartum in lactating Egyptian buffaloes delayed the resumption of estrous and ovarian activity and decreased pregnancy rate, antioxidant status, thyroid and reproductive hormones and trace elements s...
Asmaa S. Mohammed*, Ahmed F. Abou-Elnaga, Ahmed I. Ateya, Mohammed M. Fouda, Ragab A. Darwish, Usama A. Abou-Ismail
...mone receptor (FSHR) and estrogen receptor alpha (ER-α) genes in female rabbits. A total of 84 Nulliparous Blanc de Bouscat does were randomly allocated into one of four experimental groups (non-UVB, 12-h UVB, 8-h UVB and 4-h UVB) according to the duration of exposure to UVB radiation (n = 21 for each group). Behavioural patterns of rabbits were monitored for six consecutive weeks whereas, reproductive performance, expression profile of FSHR and ER-...
Asim Faraz1, Muhammad Yaqoob2, Nasir Ali Tauqir3, Hafiz Muhammad Ishaq1, Ayman Balla Mustafa4, Amir Ismail5, Muhammad Arslan Akbar6, Abdul Waheed1* and Muhammad Shahid Nabeel7
...medary camel. Mean serum estrogen concentration (P<0.05) was found to be 25.28±2.71, 54.80±2.52 pg/ml at 14th and 21st day post mating respectively in pregnant group of animals, while in lactating non pregnant animals E2 level was recorded as 10.38±1.52 pg/ml. Hormonal biomarker values were found to be significantly higher in G2 than G1. Present findings are suggestive of serum progesterone and estrogen
Genevieve R. Tabon1,2, Noraine P. Medina2, Mary Rose D. Uy-De Guia3, Claro N. Mingala2,3*
Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6
...ntly (p<0.05) whereas estrogen, follicle-stimulating hormone and luteinizing hormone decreased significantly (p<0.05) in the non-vaccinated group compared to vaccinated and control groups in both seasons. The study outcomes shown that climate has neither significant effect on reproductive physiology nor exaggerated the effect on an experimentally infected animal. Furthermore, it is concluded that pneumonic mannheimiosis causes a negative impact on reprod...
Ali Basim Abd Al-Hadi*, Tahreer Mohammed Al-Thuwaini, Mohammed Baqur Sahib Al-Shuhaib
Muhammad Ismail1, Jabbar Khan1*, Zahid Rauf2, Khalid Khan3 and Muhammad Rafi1
...ible polymorphism in the estrogen receptor (ESR1) gene. Blood samples in this regard were collected from clinically diagnosed eighteen breast cancer patients, all possessing invasive ductal carcinoma. DNA was amplified through PCR for 2 different sequences of ESR1 gene. Twelve normal individuals of different ages were also characterized. Amplified products were digested with Xbal and Pvull restriction enzymes and were electrophoresed to visualize single nucleo...
Jiahua Peng1,2, Shiyao Hua3* and Qian Wu4
... microbiological axis of estrogen and metabolic defects in SD rat model of polycystic ovary syndrome (PCOS) induced by DHEA, and to explore the possible mechanism. Forty rats were randomly divided into four group, the model groups each of 10 the control group, the DHEA group (low dose, 15 μg/kg/d) and the DHEA group (high dose, 30 μg/kg/d). DHEA subcutaneous injection was used to construct the PCOS model of the polycystic ovary syndrome (PCOS) model of r...
Maha Mostafa1, Sohail Soliman1, Reham I. Mohamed2 and Wael M. El-Sayed1*
...h as synthetic steroids, estrogens, progestins, androgens, chemical compounds, and natural plant extracts. The aim of this approach is to lower the population size of pests by reducing the natality rather than by increasing mortality. Male (20) and female (40) albino mice were orally administered testosterone at 25 mg/kg for 35 days. The males were allowed to mate with females to estimate the fertility index. After the end of treatment, mice were sacrificed, b...
Shah Murad Khan1, Hamayun Khan1, Younas Muhammad1, Haq Aman Ullah1*, Muhammad Tariq Tunio2, Ali Gohar2 and Fazli Rabbani3
...s. Results showed higher estrogen (E2) and lower progesterone (P4) concentrations for MSS than POP group. Cows with higher E2 and lower P4 levels at estrus got conceived (P<0.05). Highest level of E2 was recorded in pregnant cows in POP group in comparison with MSS. The P4 concentration of pregnant cows in MSS was lowest at the time of insemination (P<0.05). Induction of fertile estrous was maximum in POP group than MSS, but conception rate in cows recei...
Yang Hu, Jingxin Ding, Beilei Xu, Xiangming Sun, Guoyu Li, Hui Song, Zheng Zong and Wenlan Li*
...d food raw material with estrogen-like effect; its active ingredients are glycosides. To explore the absorbed constituents of total glycosides (TGs) in vivo and its estrogen-like activity, and reveal its direct acting substances in the body, in this study, 30 female Wistar rats were orally administered with TGs, and serum fingerprints of blood samples collected at 10 different times were established by UPLC-Q-TOF-MS analysis...
Tariq I. Almundarij
...spective controls, their estrogen levels were significantly different at P<0.01 at age ≥ 8. Malondialdehyde (MDA) levels in repeat breeder female Majaheem and Waddah camels with a BCS over 4.5 were compared to levels in healthy female camels and revealed that the MDA levels in the two breeds were considerably higher (P<0.05). When compared to the corresponding controls with a BCS of 4, the catalase (CAT) values in overweight female camels with a BCS o...
Hayder S. Rwayyih*, Tahani S.S. Al-Azawi
...papillary muscles due to estrogen deficiency, irregular myocardial endothelial degeneration and congested blood vessels. The aorta of these groups has enlargement and thickening of endothelial cells with infiltration of fatty cells in tunica intimae (The structure of intima changes in postmenopausal and it becomes multilayered due to migration of smooth muscle cells from the media to intima). In conclusions, the result of the current study could prove that Alp...
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