Maryyum Khalil1, Hamda Azmat1,*, Noor Khan1, Arshad Javid2, Ali Hussain1, Syed Makhdoom Hussain3, Asim Ullah1 and Sumaira Abbas1
...xogenous enzyme (α-amylase) added feed on growth performance, health and hematological analysis of Pangasianodon hypophthalmus (initialaverage weight 32.46±0.36 g). The 40% crude protein diet was prepared by adding feed ingredients along with 0.75, 0.50, 0.25, 0.00 g kg-1 of α-amylase as T1, T2, T3 and T4 (control). At the end of feeding trial, 100% survival rate was recorded. Growth rate...
Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**
BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...al esterases (TE), alpha-amylase, glucoamylase, alkaline phosphatase (AkP), acidic phosphatase (AcP), total protein, soluble protein and free amino acids (FAA). The sixth instar larvae of T. castaneum were released and exposed for 48h without food on abamectin treated glass petri dishes. The surviving ones were then homogenized in saline and centrifuged prior to biochemical analyses. Results showed differences in the activit...
Sabah Mansoor1, Muhammad Tayyab1,*, Amna Jawad1, Bushra Munir2, Sehrish Firyal1, Ali Raza Awan1, Naeem Rashid3 and Muhammad Wasim1
...n of recombinant α-amylase (AMYSBS) from Geobacillus SBS-4S. AMYSBS exhibited a highest identity of 99.78% with Geobacillus thermoleovorance GTA. E.coli BL21-CodonPlus (DE3) cells were used as host for expression studies of AMYSBS. Recombinant AMYSBS produced as inclusion bodies was transmitted to soluble active form by denaturing the insoluble protein using 8M urea followed by refolding th...
Partial Characterization of α-amylase Produced from Aspergillus niger using Potato Peel as Substrate
Shahzad Mahmood1, Memuna Ghafoor Shahid1, Muhammad Irfan2, Muhammad Nadeem3*, Quratulain Syed3
...duction of α–amylase. The crude enzyme produced was further characterized. The results showed that enzyme had an optimum pH of 6.0 and stable in range of 4.0-6.0. Optimum temperature of enzyme was 40oC and attained 96% activity at 40oC for 70min. Metal profile showed that α-amylase activity was enhanced in the presence of Ca2+, Cs1+, Mn2+ and Co
Farah Rauf Shakoori1,*, Anum Feroz1, Ayesha Gondal1,Sahar Akram2 and Tanzeela Riaz3
...zymes, the activities of amylase and invertase were significantly reduced while activity of trehalase were significantly increased after treatment with sub lethal dose of λ-cyhalothrin as compared to control. The metabolic derangements induced by sub-lethal dose of λ-cyhalothrin suggest that infestation caused by T. granarium in godowns could be overcome by calculating lethal dose of λ-cyhalothrin.
...
Farah Rauf Shakoori1,*, Tanzeela Riaz2, Uzma Ramzan1, Anum Feroz1 and Abdul Rauf Shakoori2,3,*
...activities of trehalase, amylase and invertase were significantly reduced after treatment with sub lethal dose of esfenvalerate as compared to control. The metabolic derangements induced by sub-lethal dose of esfenvalerate suggest that infestation caused by T. granarium in godowns could be overcome by calculating lethal dose of esfenvalerate.
...
Muhammad Asam Riaz1, Javeria Choudhary1, Muhammad Zeeshan Majeed1*, Muhammad Afzal1, Sohail Ahmed2 and Muhammad Sajjad Khalil1
... increased activities of amylases with Vmax value of 2.68 and 1.89 µM s-1 respectively and Km value of 23.8 g L-1 and 40.1 g L-1, respectively. These findings suggest that the increased activities of cellulases and amylases may play a major role in mitigating fitness-cost associated with insecticide resistance. ...
Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
Mirfa Murtaza1, Sajid Abdullah1, Wardah Hassan1*, Khalid Abbas1, Huma Naz1, Muhammad Anjum Zia2
Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
...
polish. The activity of amylase and lipase was higher in all fish species fed with corn gluten. Liver showed higher
amylase activity than the intestine, while the activity of lipase was higher in intestine. It was concluded that different
fish species respond differently to various feed ingredients. Therefore it is important to follow the acceptability and
digestibility standards during the selection of...
amylase activity than the intestine, while the activity of lipase was higher in intestine. It was concluded that different
fish species respond differently to various feed ingredients. Therefore it is important to follow the acceptability and
digestibility standards during the selection of...
Atiq-ur-Rehman1,2,*, Abida Latif1,*, Rukhsana Anwar1, Nasir Abbas1, Sajid Nawaz2 and Hamid Turab Mirza3
...for to determine α-amylase inhibition for the evaluation of hypoglycemic potential of the plant. So, the aim of this study was for the investigation of in vitro, in vivo anti-diabetic potential and mode of action of various fractions of methanolic extract of the aerial parts of this plant. Various fractions (n-hexane, chloroform, ethyl acetate and n-butanol) from the most active methanolic extract were prepared. Hypoglycemic potential was i...
Wang Zaigui1,*, Guo Panpan1, Ye Miao1, Sun Linghong1, Zhang Hongfu2 and Liu Chaoliang1,*
...nces, cocoon quality and amylase activity of silkworm, Bombyx L, we got the following results: (1) In the larval duration of 3rd instar silkworm, average weight gain (AWG) and feed conversion ratio (RFC) were not significantly affected with the use of B. subtilis, but the remaining amount of mulberry leaves (RAML) was significantly decreased contrasting to the blank group (P<0.05). (2) In the larval duration of 4th i...
Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3
...different enzymes (alpha-amylase, cellulase and pectinase) from aqueous system has been studied by applying novel concept of unidirectional freezing. To separate the enzymes from their aqueous system, directional freezing was applied horizontally through synthetic enzyme contaminated water samples, called radial freezing. After complete freezing, the samples were collected from various sections of the frozen mass and concentration of enzymes was determined. It...
Parsa Riaz and Muhammad Naeem*
Digestive Enzymes Activity with Gut Morphometric Parameter of Carnivorous Fish Wallago attu (Siluridae, Siluriformes)
...s lipases, proteases and amylases in the digestive tract of a wild, freshwater, carnivorous Wallago attu. Wallago attu is a fast growing catfish that belongs to family siluridae under the order siluriformes. It has good market demand as a food fish having high nutritional value, and high protein content in its flesh, Carnivorous in nature. Descriptive data of the studied traits included total body weight, total body length, gut weight, gut length, condition fa...
Taqi Raza1*, Sergio de Los Santos Villalobos2, Muhammad Shehzad3, Shakeel Imran4 and Derly José Henriques da Silva5
PGP Characterization of Rhizobacteria Associated with Apple Gourd (Praecitrullus fistulosus L.)
...ed strains confirmed the amylase and pectinase production activities and 36% confirms the protease activities. Similarly, 82% and 45% of isolates confirmed the solubilization of zinc (Zn) and phosphorus (P) respectively. Almost, the entire isolated strains were given positive results for ammonia (NH4), nitrogen fixation, and indole acetic acid (IAA). Furthermore, for exopolysaccharide (EPS) test, 73% of isolated strains confirmed the EPS production. The conclu...
Jen-Hong Chu1, Ya-Wen Lan2, Shyn-Shin Sheen3 and Angela Chien3*
...dietary lipid level, but amylase activity was not significantly affected by the dietary lipid level. Based on a broken-line of the final weight of L. canarium larvae, the optimum dietary lipid levels were 5.6%.
...
Tanzeela Riaz1, Farah Rauf Shakoori2*, Syed Shahid Ali2 and Mushtaq Ahmad Saleem1
...ced, while trehalase and amylase activities were significantly increased in larvae of both populations throughout the phosphine exposure period with reference to their respective unexposed group values. In case of soluble proteins, an elevation was observed which was followed by a decline after 72 h. Similarly, free amino acid contents showed increasing trend in both larval instars of tested populations throughout the exposure period with the exception of the ...
Sadaf Ashraf1, Masood Ahmed Siddiqui1*, Kanwal Nisa1, Samar Ali1 and Naeem Rashid2
...xhibited optimal α-amylase activity at 85°C and pH 5.5. The activity was not dependent on any metal ion. The hyperthermophilic nature and metal ion independence make it a suitable candidate for both basic as well as applied research.
...
Shoaib Ahmed Pirzado1,2*, Wu Zhengke1, Adanan Purba1, Chen Jiang1, Huiyi Cai1, Chen Guilan1 and Guohua Liu1*
...ments. Moreover, jejunal amylase and trypsin activity were improved in AZO 0.25% and 0.50% supplemented groups. CREAT was significantly increased in AZO 0.50% treatment, while IgG was significantly improved in AZO 0.25% treatment. Tibia diameter (TD), tibia breaking strength (TBS) were numerical higher in AZO 0.25%, while Ca and P % was significantly (P<0.05) increased in Azomite 0.25% treatment. The results indicated that addition of azomite at the dose of...
Laiba Shafique1*, Mahroze Fatima2, Syed Zakir Hussain Shah3, Muhammad Afzal4, Huma Naz5, Saif ur Rehman1 and Qingyou Liu1*
...sult for the activity of amylase, protease and lipase. Finally, formic acid and vitamin D3improves bone mineralization and digestive enzymes activity in the C. idella....
Momotaz Khanom*, Sheikh Mustafizur Rahman, Muhammad Yousuf Ali, Md. Shahin Parvez, Al-Hasan Antu, Md. Nazmul Ahsan
... to significantly higher amylase activity in comparison to other feeds, while protease activity was comparable among the feeds except for DT. The results suggest that formulated feed can be used as a potential substitute for live Tubifex for the early stage of larvae of stinging catfish.
Keywords | Enzyme activity, Formulated feed, Growth performance, Stinging catfish, Tubifex
...
Ali Ahmad1*, Zubair Aslam1, Korkmaz Bellitürk2, Ehsan Ullah1, Ali Raza1 and Muhammad Asif3
...lose, proteases, lipase, amylases, chitinase), which degrade organic substrates even after they have been secreted. Earthworms change the chemical and physical characteristics of organic substrates, over time reducing the C:N ratio, increasing the surface area exposed to bacteria, and improving the microbial degradability of organic materials. This study aims to demonstrate how organic amendments, rather than chemical fertilizers, might improve soil health and...
Uzma Hameed1*, Aneela Liaqat1, Muhammad Asjad Khan2, Ikram-Ul-Haq1 and Maham Aslam1
...align: justify;">The ⍺-amylases are primary industrial amylases with the expected global market to grow at a CAGR (Compound Annual Growth rate) of 7 % during 2021-2028. Due to widespread industrial applications, alpha-amylases are of continuous research focus. The present work depicts the enhanced alpha amylase bioproduction by Bacillus subtilis by opt...
Nazish Manzoor1*, Alim Khan1, Muhammad Yaseen1, Abid Ullah1, Zulqarnain2 and Iftikhar Jan2
Müge Aliye Hekimoglu and Sırma Sönmez
...zymes (protease, lipase, amylase), weight and length, body colour and survival rate. The image analysis method was used to determine skin colour. The chemical analysis method was used to determine the activity of enzymes. The results showed that supplementation of 2000 mg/kg of dietary significantly increased weight, length and specific growth rate (P < 0.05). While no change in colour was observed in the control group at the end of the experiment, it was d...
Muhammad Adeel Farooq1, Shaukat Ali1*, Ali Hassan1, Rida Sulayman1, Muhammad Ahsan Kaleem2, Hafsa Shahzad1, Muhammad Summer1, Arooj Latif1, Tahreem Tanveer1
... imperative enzyme alpha-amylase is used in various commercial and research applications. Due to the high market demand for α-amylase, the production of this enzyme was increased in the current study employing a mutation in wild strains. To reduce costs, less expensive agro-wastes were used, such as soybean meal, wheat bran, apple peels, rice husk, and cucumber peels. The mutant strains of Bacillus subtilis (BSAA-5 to ...
Ummul Firmani1,3, Rahmi Nurdiani2, Arning Wilujeng Ekawati2 and Happy Nursyam2*
...acteria could produce an amylase enzyme with an amylolytic index of 5.16, identified as Bacillus paramycoides. This bacteria also synergized with other bacteria and did not have hemolytic activity on blood agar media. The results of the antagonistic test based on the well-diffused method against Aeromonas hydrophila showed that B. paramycoides did not produce an inhibition zone around the bacterial wells. The molecular identification found that the bacterial s...
Irfan Safdar Durrani*, Noreen Asim and Ammar Sohail
...nbare promoter and Alpha-amylase and B3 in Dilrosh-97 promoter were observed abundantly close to the transcriptional initiation site. Expression analysis using TENOR identified that OsGLP12-3 gene show high to minimal level of expression in response to different stress stimuli. Phylogenetic analysis of the Dilrosh-97 promoter sequence showed close relationship of this promoter with the Japonica rice cultivar Nipponbare. Three transcription factors including B3...
Hanaa H.A.Gomaa1, A. F. Moustafal, Kh.A.El-Dougdoug2, A.A. Abou-Zeid3 and S. Y.M. Mahmoud4
...ed hydrolytic enzymes of amylase and protease were reduced in infected potato plants while the level of polyphenol oxidase and peroxidase was increased. PVX and PVY infected potato plants contained less auxins and gibberellins, and the pattern of growth promotors and growth inhibitors was altered. Cytokinin was also inhibited as a result of virus infection. Examination of epidermal strips of infected potato plants revealed the presence of cytoplasmic inclusion...
Pramudya Andiana*, Moch. Geerhan Miraja Syahdan, Khothibul Umam Al Awwaly, Abdul Manab
...ing ability, and α-amylase inhibitory activity. CHP1 showed the best bioactivity with a DPPH IC50 value of 4.62 mg/ml, iron chelating ability of 81.07%, copper chelating ability of 35.97%, and α-amylase inhibitory activity of 32.67%. While the best reducing power was at CHP2. As a result, it can be concluded that the use of a combination of bromelain and papain enzymes in chicken head protein hydrolysate has the ...
MUHAMMAD NADEEM1, MUHAMMAD WASEEM MUMTAZ1&MUHAMMAD DANISH1
...ant activity and α-amylase inhibition potential of hydroethanolic leaf extracts of Vitexnegundo. Freeze drying assisted ultrasonicated extraction revealed highest level of total phenolic contents (258.29±2.52 mgGAE/g DE) and total flavonoid content (155.91±1.75 mgRE/g DE) in 60% hydro-ethanolic V. negundo leaf extract. Furthermore, total antioxidant power (254.35±1.56 mg AAE/g DE) and antidiabetic effectbased on α-
Biologia (Lahore)
December
Featuring
-
Quantification of Sodium from Food Sources by Using Various Analytical Techniques
Nauman Jamil Khan, Rabia Tariq, Hina Saleem and Muhammad Waheed Mushtaq
Biologia (Lahore), Vol.69, Iss. 2, Pages 66-75
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Studies on the Occurrence of Prostate Cancer in Gujrat, Punjab, Pakistan and its Association with Testosterone Level
Kanwal Nisa, Sadia Roshan, Shazia Shamas, Raheela Atta Mustafa, Shamaila Irum, Kalsoom Sughra and Memoona Iqbal
Biologia (Lahore), Vol.69, Iss. 2, Pages 54-60
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Prevalence of Gastro-Intestinal Parasites in Pigeons of District Jhang-Pakistan
Muhammad Fiaz Qamar, Tahir Hussain, Iram Liaqat, Madiha Kiran, Abdur Rahman Ansari, Faiza Yasmeen and Tahira Batool
Biologia (Lahore), Vol.69, Iss. 2, Pages 48-53
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Phytochemical Screening and In Vitro Antioxidant Activity of Extracts of Ipomoea purpurea Leaves from Iran
Fatemeh Beheshti, Maliheh Safavi, Mohammad Reza Akbari Eidgahi, Parviz Kokhaei, Mehdi Vazirian and Ali Akbar Shabani
Biologia (Lahore), Vol.69, Iss. 2, Pages 32-39
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