Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
Life Sciences International Journal Issue 7 Volume 1
Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
British Journal of Virology
Muhammad Arif Zafar1*, Arfan Yousaf1, Murtaz-ul-Hasan1, Faisal Ayub Kiayani1, Matee ul Islam2 and Muhammad Farooq Iqbal1
...r, hand-sewn, end-to-end anastomosis technique in a calf treated for traumatic umbilical hernia with peritonitis. A cross-bred calf was presented with peritonitis due to traumatic umbilical hernia. The decision to operate the calf was made after confirmation of umbilical hernia resulted in septic shock. Animal was first stabilized with supportive treatment prior to surgical decompression. A modified two-layer hand-sewn anast...
Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1
...s isolated from Shaukat Banaspati Ghee industry (Grw) and Shezan foods (pvt) Ltd, Lahore, Pakistan food industry, 10 highly antibiotic resistant strains were subjected to morphological, physiological and biochemical characterization. 16SrRNA sequencing was performed to identify three highly resistant strains at species level. Antimicrobial activity of aqueous and methanolic plant extracts of Camellia sinensis (Green tea), Syzygium aromaticum (Clove) and Mentha...
Farah Rauf Shakoori, Momina Shokat, Faiza Saleem and Tanzeela Riaz
...lign: justify;"> Xylanase enzyme is of great importance commercially because of its high stability at elevated temperatures and in alkaline conditions. The present study was aimed to isolate and screen xylanase producing bacteria and to optimize cultural conditions for maximum production of enzyme from locally isolated bacteria and to optimize the assay conditions under which enzyme possessed maximum activity. Two bacte...
Khalid Mahmood Qureshi, Fakhar ul Hassan, Qamar ul Hassan, Usman Shaukat Qureshi, Saman Chughtai and Adnan Saleem*
IMPACT OF CULTIVATION SYSTEMS ON GROWTH AND YIELD OF STRAWBERRY (FRAGARIA ANANASSA) CV. “CHANDLER”
...e strawberry (Fragaria ananassa) cv. Chandler were collected from Swat (Mingora.) Different cultivation systems were adapted i.e., standard growing media in polyethylene bags (T ), soil less media (T ), high tunnel (T ), green house (T ), 1 2 3 4 including open field condition on ridges (T ) by keeping plant to plant 5 distance of 30 cm and row to row distance of 60 cm. The study was conducted to evaluate the effects and suitability of different cultivation sy...
Sara Khalid*, Khalid Mahmood Qureshi*, Ishfaq Ahmad Hafiz*, Khalid Saifullah Khan* and Usman Shaukat Qureshi*
EFFECT OF ORGANIC AMENDMENTS ON VEGETATIVE GROWTH, FRUIT AND YIELD QUALITY OF STRAWBERRY
...n strawberry (Fragaria ananassa Duch.) cv. Chandler which included T = planting 1 media -1 (soil + silt + farm yard manure); T = planting media + 400 mgl humic 2 -1 acid; T = planting media + 200 g kg leaf manure; T = planting media + 200 3 4 -1 -1 g kg vermicompost; T = planting media + 200 g kg plant fertilizer and T = 5 6 -1 planting media + 200 g kg bio-compost during 2011-12 at PMAS-Arid Agriculture University, Rawalpindi. Treatment T (soil + silt + FYM) ...
Rabeea Tariq*, Khalid Mahmood Qureshi*, Imran Hassan*, Muhammad Rasheed* and Usman Shaukat Qureshi*
EFFECT OF PLANTING DENSITY AND GROWING MEDIA ON GROWTH AND YIELD OF STRAWBERRY
...p;Strawberry (Fragaria ananasa), belonging to Rosaceae family, is a rich source of vitamins and minerals with delicate flavors. It is perishable crop which is exceedingly in demand for its taste, profitability, high yield and good quality. To make the plant growth successful in the container, the requirement of special media is very important step because plant growth is largely depended on the physiochemical properties of the growing media used. Winter strawb...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
Habib-ur-Rehman1, Masood Ahmed Siddiqui1,*, Abdul Qayyum1, Arifa Bano1 and Naeem Rashid2
...
...
SiRui Wang1,2, Fekede Regasa Joka1,2, XiaoLong Wang1,2,* and SuYing Bai2,*
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...es comprising from 23 Japanase quails and 22 chickens were analyzed by conventional and molecular methods for E. coli isolation in this study. Subsequently, antimicrobial susceptibility profiles of E. coli isolates (n=71) were determined against thirteen agents; amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftazidime, cefotaxime, ceftriaxone, cefpodoxime, aztreonam, ciprofloxacin, amikacin, ertapenem, imipenem, meropenem, trimethoprim-s...
Azeem Haider1, Muhammad Mohsin Alam2*, Azhar Ali Khan3 and Muhammad Asif Zulfiqar3
...ifferent enzymes viz; xylanase, CMCase and laccase production by Pleurotus ostreatus were optimized in shake flask cultures. The results indicated that the optimum fermentation medium in shake flask studies contained a carbon (glucose 4%), nitrogen (0.5% ammonium chloride), inoculation level (three disks, 0.5 cm in diameter), temperature (30 °C), incubation period (at 7 days) and initial pH 5.0. Under these culture conditions, the maximum level of xyl
Muhammad Suleman1,*, Abu ul Hassan Faiz2, Muhammad Shahbaz2 and Ayesha Riaz3
... on the production of xylanase. Submerged fermentation was carried out in 500 ml Erlenmeyer flasks using vogel’s medium at 35°C for five days. UV irradiated A. niger has significantly increased the xylanase production as compared to parent strains. Twenty minutes exposure of A. niger was considered suitable for the production of xylanase. Wheat bran concentration (3...
Beenish Zahid*1,2,7, Javed Iqbal Qazi2, Amir Zohaib2, Asim Aslam3, Raheela Akhter3, Haleema Sadia4, Qurat ul Ain5, Razia Sultana6, Irfan Irshad3 and Sobia Alyas7
Tanzeela Riaz, Fiza Sana-Ullah Khan, Farah Rauf Shakoori
...roduction.
...
Hamoudi Naoual1, Alloui Nadir2*, Barberis Abdelhak2 and Boudaoud Amine2
Dilara Abbas Bukhari*, Mehwish Faheem, Shamoona Arshad and Khalid P. Lone
... dietary consumption of Banaspati ghee on renal function of mice. Two locally manufactured brand of Banaspati ghee daily to mice at a rate of 10% of their daily food intake for a period of 4 weeks. After 4 weeks, both male and female mice were sacrificed and kidneys were taken out. Biochemical parameters e.g., lipid peroxidation (LPO), catalase (CAT), glutathione-S-transferase (GST), reduced glutathione (GSH) were determined...
Kifayat Ullah*1, Tahir Mahmood2, Naeem Jan3, Zeeshan Ali4
A NOTE ON GEOMETRIC AGGREGATION OPERATORS IN T-SPHERICAL FUZZY ENVIRONMENT AND THEIR APPLICATIONS IN MULTI-ATTRIBUTE DECISION MAKING
... in fuzzy mathematics. Atanassov’s
intuitionistic fuzzy set (IFS) and Cuong’s picture fuzzy set (PFS) are two featured fuzzy concepts. Recently, a novel
framework of T-spherical fuzzy set (TSFS) and consequently spherical fuzzy set (SFS) are developed for handling
those problems where uncertain situations have more than two aspects. This manuscript is based on some contribution
to the area of SFS and TSFS. In this manusc...
intuitionistic fuzzy set (IFS) and Cuong’s picture fuzzy set (PFS) are two featured fuzzy concepts. Recently, a novel
framework of T-spherical fuzzy set (TSFS) and consequently spherical fuzzy set (SFS) are developed for handling
those problems where uncertain situations have more than two aspects. This manuscript is based on some contribution
to the area of SFS and TSFS. In this manusc...
Yuan Xing, Congming Tan, Yan Luo and Wenjun Liu*
... (10 mg / kg, p.o.). Intranasal OVA has been instilled on the 21 days. Biochemical parameters, spleen weight, physiological parameters, interleukin (IL-1β and IL-6) parameters and immunoglobin-E (IgE) were calculated at the end of the experimental study. To investigate the potential mechanism of action, Paw edema and mast cell de-granulation are estimated. Used to measure immune and inflammatory mediators, qRT-PCR technique. Quercetin significantly (P<...
Shikang Deng1,2, Yan Jin2, Jing Xu2, Xiufang Zhu2, Pinghai Hu2, Jiao Li2, Li Zhang2 and Jianzhong Tang2,*
...plicated by incision and anastomosis in miniature pigs and bile duct scar fibroblasts were obtained. After cell identification, the bile duct scar fibroblasts were given different concentrations of 5-FU (0, 0.001, 0.01, and 0.1 mmol/L). After 48 h of intervention, CCK-8 method was used to detect the cell proliferation level of each group; Western blot was used to detect the expression of TGF-β1, cyclinD1, and CDK4 protein in each group of cells; and real-...
Abdul Nabi Jatt1,2*
...cellulase enzyme (exoglucanase and endoglucanase) activity was highly induced in addition to exogenous AHLs. While, both exoglucanase and endoglucanase activities were radically decreased, when the growth medium was supplemented with quorum-quenching (QQ) AiiA protein. The present study, for the first time provides evidence that the QS system via AHL mol...
Saleem Maseeh Bhatti1*, Muhammad Aslam Panhwar1, Zohaib ur Rehman Bughio1, Muhammad Saleem Sarki1, Allah Wadhayo Gandahi1, and Niaz Ahmed Wahocho2
...strawberry (Fragaria x ananassa cv. Silder) at Nasarpur, Sindh, Pakistan during 2015-16. The foliar rates comprised of 33, 66, and 99 mg Zn L-1 (equivalent to 100, 200 and 300 mg ZnSO4 L-1) and distilled water (control treatment) and sprayed to strawberry plants before flower initiation. A continual increase in number of leaves, number and weight of berries, fresh weight of strawberry plants, and Zn concentration in strawberry fruit was observed as a function ...
Shimaa A. Khalaf1 , Mohsen M. Elsharkawy2, Samir A. Sidaros2, Shawky A. El Kewey2, Ayman F. Omar2, Ali Hamed1
...hitinase, β 1-3 Glucanase, PR1, POX, PAL and LOX) explaining the role of these genes in the mechanism of induced resistance against PRSV by GU23-3.
Conclusion: GU23-3 could be considered a new and alternative strategy to control PRSV infection.
... 
Ilker Kepenekci1, F. Dolunay ErdoÄźuĹź2 and Adnan Tülek3*
Sanjeela Sabahat1*, Juliya Abbasi2, Mushtaq Ahmad1, Saima Mumtaz1, Taj Naseeb Khan1, Sudheer Tariq1 and Muhammad Imran1
...Strawberry (Fragaria x ananasa) is a widely grown fruit crop known for its color, aroma and texture. The fruit is enriched with vitamin C, manganese and antioxidants. In Pakistan, except for a few low-temperature regions, it is mainly grown as an annual fruit crop where runners are transplanted every year. Owing to its short growing period and high profitability, it is mostly produced by small scale farmers. However, the changing climate from the past few year...
Gülseren Kırbas Dogan1,*, Emin Karakurt2, Mushap Kuru3 and Hilmi Nuhoglu2
...osoma reflexum, two with anasarca, and one with nasal and calvarium openings. After necropsy, samples were taken from the organs, foreseen and routine pathological examinations were performed. Following these procedures, the calves were brought to the anatomy laboratory and anatomically examined. The most important result; in the mid-section of the sections taken from the existing structure other than the normal formation at the medulla oblangata and pons leve...
Ni Putu Vidia Tiara Timur1*, Maria Maliga Vernandes Sasadara2, Purwanta1, Anak Agung Jayawardhita3
...lis glands under general anasthesia using a combination of Ketamin and Xylacine ad the sedative agent. The dog was prescribed five days post-surgery medication using an antibiotic, an anti-inflammatory agent and supportive therapy. Regular monitoring was conducted for two months before it was confirmed that no saliva was re-accumulated.
Keywords | Salivary mucocele, Surgery, Treatment, Local dog, Bali
...
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
...lly confirmed by endoglucanase assay. Enzyme activity measured using the glucose standard curve was 725 U/ml after acetone precipitation. Thermal stability and optimum temperature were found to be 60°C for maximum activity. Likewise, enzyme was found more stable at 6.0 pH. Various mono and divalent cations were studied against the cellulolytic activity exhibited significant enhancing and inhibitory effect upon different concentrations. Effect of inhibitors...
Ghassan Zahid1, 2*, Sara Iftikhar3, Muhammad Umer Farooq4 and Shakeel Ahmed Soomro5
...les, mangoes, guavas, bananas, date palms and peaches, etc. Furthermore, by offering a piece of scientific information and addressing developments, notably in DNA-based molecular markers, this review paper may serve as a road map for obtaining high-yielding fruit varieties in Pakistan....
Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6
...oups were challenged intranasally with 2 mL of bacterial cell suspension containing 105 colony-forming unit (CFU) of M. haemolytica serotype A2. Blood samples were collected on weekly basis. The results revealed that the plasma progesterone concentration increased significantly (p<0.05) whereas estrogen, follicle-stimulating hormone and luteinizing hormone decreased significantly (p<0.05) in the non-vaccinated group compared to vaccinated and control gro...
Rida Haroon Durrani1, Ali Ahmad Sheikh1*, Masood Rabbani1, Muti-ur-Rehman Khan2, Aneela Zameer Durrani3, Salman Ashraf1, Muhammad Anas Naeem1, Muhammad Usman3 and Tauqeer Mahmood4
...at received cocktail intranasally showing log100.85 on the 3rd day. As a delayed treatment, oral delivery significantly (P ≤ 0.05) reduced count with a mean log101.2 and log101.55 on 3rd dpc, with efficiency decline on 6th dpc. No reduction was recorded for nasal delivery test subjects in delayed treatment group. As an immediate intervention, the oral delivery reduced bacterial count to log100.86 and log101.02 on the 3rd dpc. Phage-cocktail is promising pre...
Qingyue Han1, Ying Li1, Jichang Deng1, Kunxuan Huang1, Yanyang Yang1, Quanwei Li1, Zhuowei Zhang1, Na Qiao1, Yanju Ji2, Khalid Mehmood3, Sarfaraz Ali Fazlani4, Hui Zhang1 and Zhaoxin Tang1*
...h after birth. After euthanasia, heart was collected for histopathological examination and IGF, IGF1, IGF2, IGFBP3, PCNA and ki67 expression were analyzed. The results showed that low calcium supplementation decreased the cardiac index and development of myocardial muscle fiber, while vitamins D3 can improve the decrease of cardiac index and development of myocardial fiber due to low calcium supplementation. The expression profile of IGF1, IGF2, IGFBP3, PCNA a...
Lei Zeng1,2,3,4, Pimao Chen1,2,3,4, Zhenzhao Tang1,3,4, Jie Yu1,2,3,4 and Guobao Chen1,2,3,4*
...ristatus, Charybdis callianassa and Harpiosquilla harpax, showing a distinct preference tropism to AR, were turned out to be the main factors contributing to the spatial heterogenity of nekton community between A and B, which was closely related to dissolved oxygen (DO), water transparency (Tra) and total inorganic nitrogen (TIN). The findings in this paper are significant for the protection and restoration of crustaceans resourcs along coastal water, given th...
Siti Dharmawati1, Muhammad Halim Natsir2, Hartutik Hartutik2, Danung Nur Adli2, Osfar Sjofjan2*
A.H. Choshali1, S. Rezaee2, S. Jamali3, H. Reza3, Zamanizadeh4 and F. Rejali5
...ase and ß 1, 3 glucanase activities in susceptible and tolerant cucumber roots pre-colonized with Funneliformis mosseae against root knot-nematode Meloidogyne incognita were studied. Mycorrhizal plants which pre-colonized for 7 weeks inoculated with 1500 J2 per 1 kg soil. The quantitative activity of chitinase and ß 1, 3 glucanase enzymes was assessed on 2, 4, 6 and 8th days after M. incognita inoculation based o...
Lihang Wang, Qiling Chen, Tingsheng Lu, Shudan Yao, Xingwei Pu and Chunshan Luo*
...d tissues was behind euthanasia of rats. Detection of biochemical indicators, observation of cell apoptosis, and determination of Bax, Bcl-2, p38 MAPK and Smad2 were clarified. We found that MH and MH+MPS could effectively reduce apoptotic cells, dramatically minify TNF-α, IL-6, IL-8, MDA and Bax contents (P < 0.01) and elevate GSH-Px and Bcl-2 level (P < 0.01). Additionally, the P38AMPK/Smad2 pathway proteins (phosphorylated p -P38MAPK and Samd2) ...
Fathin Faahimaah Abdul Hamid1, Mohd Farhan Hanif Reduan1*, Jasni Sabri1 , Faez Firdaus Jesse Abdullah2,Mohammed Naji Odhah1, Nur Athirah Binti Abdul Manaf1, Mohd Jefri Norsidin2 , Siti Nor Che Yahya1, Intan Noor Aina Kamaruzaman1, Nur Zul Izzati Mohd Rajdi1
...ation was inoculated intranasally to all goats except goats of Group 1 which served as the negative control, Group 2 was the positive control, Group 3 goats treated with oxytetracycline, Group 4 goats were treated with flunixin meglumine, and group 5 received both oxytetracycline and flunixin meglumine treatments. Blood samples were collected at 24 hours, days 5, 7, 9, 11, 14, 21, and 28 post-inoculation for the clinical pathology evaluation through complete b...
Sami Ullah1, Choudary Ihtasham Ali1, Umar Ijaz Ahmed1*, Shoaib Nasir1 and Amjad Masood2
...ch is commonly known as vanaspati ghee. Over time, due to changing eating preferences, health concerns, and quality issues, vegetable oil is rapidly replacing vanaspati ghee in the country. This study has investigated the impact of taste and health preferences and other factors on the choice of cooking medium. In this study, we have utilized a well-structured and pre-tested questionnaire for data collection from 250 responde...
Muhammad Syarif Djaya1, Osfar Sjofjan2, Hartutik Hartutik2, Irfan Hadji Djunaidi2
Fariha Javaid1*, Zahoor Qadir Samra1, Madeeha Shahzad Lodhi1,2, Aroosha Hussain1 and Gulnaz Pervaiz1
...eel, and leaf parts of Ananas comosus. Protease accounts for 60% of the total enzyme market. Being a plant-based cysteine protease, it has various pharmaceutical and biotechnological applications. The isolation and purification cost of the enzyme is highest, so there is a need to develop cost-effective purification methods. This research work aims to purify BRM by using magnetic nanoparticles. After purification, BRM enzyme was characterized and utilized in th...
Mohammed A. Ali1*, Firas A. Alhasson1, Thaer A. Mohsin2
Ahmed Abd El Samie Hassan Ali
...lated simultaneously intranasal, oral and intraperitoneal into 6 New Zeeland rabbits. The rabbits were observed for 25 day, slaughtered then tissue harvests from spleen, lung, lymph nodes and intestine were pooled, homogenized; the supernatant was clarified and concentrated for second inoculation. A another 16 BVDV seronegative New Zeeland rabbits, classified into 4 groups, each of 4 designated for second closed inoculation with clarified supernatant of rabbit...
Ahmed Abd El-Samie H. Ali
...groups were infected intranasally with a virulent NCP BVDV type I (NY-I) and 2 (890) biotypes and kinetic evaluation of its effect of in vivo infection on clinical and immunological parameters. All virus-inoculated calves developed a fever on days 3-7-post infection. BVDV antigens were detected in the buffy coat cells on days 3, 5, and 7 post infections. Most calves developed low neutralizing antibody titers as type I (3, 12, 18 and 44) and type-2 (6, 16, 20 a...
Amina1, Muhammad Zahid Rashid1*, Muhammad Asim Rashid2 and Amina Rashid2
