Search for Cellulase

Effect of alkali treatment (NaOH) of eucalyptus leaves for hypercellulase production by Bacillus subtilis through submerge fermentation

Sidra Iqbal1, Muhammad Irfan2*, Hafiz Abdullah Shakir1, Fouzia Tabbsum1, Javed Iqbal Qazi1

...si). After pretreatment, cellulase production was conducted in 250ml Erlenmeyer flask at 50 ºC, pH 5 with shaking speed of 120 rpm using 2% pretreated eucalyptus leaves as carbon source and 2% v/v inoculum size in submerged fermentation for 24h. Results showed that among these two treatments, only chemical treated substrates gave better enzyme yield as compared to chemical followed by steam. The highest FPase (2.526 IU/ml/min) activity was found at pretre...

Optimization of dilute sulphuric acid pretreatment of peanut shells through Box- Bhenken design for cellulase production by Bacillus subtilis K-18

Fatima Arshad1, Muhammad Irfan2*, Hafiz Abdullah Shakir3, Fouzia Tabbsum3, Javed Iqbal Qazi3

...found more effective for cellulase production as compared to thermochemical pretreatment. The maximum CMCase activity was 1.757 1U/ml/min under conditions of 0.6% acid concentration, 10% substrate concentration, and resident time of 4h. The maximum FPase activity was 2.015 1U/ml/min under conditions of 0.8% acid concentration, 10% substrate concentration, and time of 6h. This showed that peanut shells could successfully be used as substrate for

Dilute Sulphuric Acid Pretreatment Optimization of Cotton Stalk for Cellulase Production through Box-Bhenken Design

Noor Fatima¹, Muhammad Irfan^2,*, Hafiz Abdullah Shakir¹, Javed Iqbal Qazi¹

...act | In this study, cellulase enzyme production was assessed by pretreating cotton stalk with dilute sulphuric acid which was optimized through response surface methodology. Pretreatment conditions were optimized using three variable with three levels like sulfuric acid concentrations (0.6%, 0.8%, 1% v/v), biomass loading (5%, 10%, 15% w/v), and residence time (4, 6 and 8h). After pretreatment process, cellulase product...

Enhanced Activities of Metabolic Enzymes Associated with Insecticide Resistance in Red Flour Beetle (Tribolium castaneum Herbst)

Muhammad Asam Riaz¹, Javeria Choudhary¹, Muhammad Zeeshan Majeed^1*, Muhammad Afzal¹, Sohail Ahmed² and Muhammad Sajjad Khalil¹

...tly higher activities of cellulases. Enzyme kinetics results demonstrated that Swl-strain had a maximum value of Vmax (68.4 Nm s-1) and lowest value of Km (12.8 g L-1). Fsd- and Swl-strains exhibited increased activities of amylases with Vmax value of 2.68 and 1.89 µM s-1 respectively and Km value of 23.8 g L-1 and 40.1 g L-1, respectively. These findings suggest that the increased activities of cellulases and amylases...

Optimization of medium and substrate for CMCase production by Bacillus subtilis-BS06 in submerged fermentation and its applications in saccharification

Mohsin Arshad1, Muhammad Irfan*2, Asad-Ur-Rehman1, Muhammad Nadeem3, Zile Huma3, Hafiz Abdullah Shakir4, Quratulain Syed3

Optimization of medium and substrate for CMCase production by Bacillus subtilis-BS06 in submerged fermentation and its applications in saccharification

...trates for carboxymethyl cellulase
(CMCase) production under static and with agitation speed of 140 rpm. The whole
fermentation experiments were carried out in 250ml Erlenmeyer flask. Different
agricultural wastes such as sugarcane bagasse, wheat straw, rice husk, defatted
soybean meal, corn cobs and wheat bran were exploited for CMCase production
using different media (medium 1 (M1), medium II (M-II), medium III (M-III) &...

Cloning, Expression and Characterization of Highly Active Recombinant Thermostable Cellulase from Thermotoga naphthophila

Aisha Khalid¹, Muhammad Tayyab^1,*, Abdual Rauf Shakoori², Abu Saeed Hashmi¹, Tahir Yaqub³, Ali Raza Awan¹, Muhammad Wasim¹, Sehrish Firyal¹, Zaheer Hussain⁴ and Munir Ahmad⁵

...recombinant thermostable cellulase from Thermotoga naphthophila. PCR using the genomic DNA of T. naphthophila as template resulted in amplification of 1 kb cellulase gene. The amplified cellulase gene was cloned in pTZ57R/T and sub-cloned in pET28a. The expression of recombinant cellulase was analyzed using BL21 CodonPlus (DE3) cells as exp...

Optimization of Conditions for Maximal Production of Recombinant Thermostable Cellulase from Thermotoga naphthophila using E. coli BL21-CodonPlus (DE3) as Expression Host

Aisha Khalid¹, Muhammad Tayyab^1,*, Abu Saeed Hashmi¹, Tahir Yaqub², Ali Raza Awan¹, Muhammad Wasim¹, Shagufta Saeed¹, Sehrish Firyal¹and Abdul Rauf Shakoori³

...oduction of thermostable cellulase. Production of cellulase was focused due to its diverse range of application in industry. In the present study, conditions were optimized for the maximal production of recombinant thermostable cellulase from Thermotoga naphthophila using BL21-CodonPlus (DE3) cells as expression host and pET28a as expression vector. Effect of various concentration o...

Separation of Enzymes from their Aqueous System by using Novel Concept of Unidirectional Freezing

Muhammad Waheed Mushtaq^1,*, Muhammad Waqar Younas², Jamil Anwar², Syed Mustansar Abbas³and Shahid Bashir³

... enzymes (alpha-amylase, cellulase and pectinase) from aqueous system has been studied by applying novel concept of unidirectional freezing. To separate the enzymes from their aqueous system, directional freezing was applied horizontally through synthetic enzyme contaminated water samples, called radial freezing. After complete freezing, the samples were collected from various sections of the frozen mass and concentration of enzymes was determined. It was foun...

Utilization of Bombyx ceiba Seed Pods: A Novel Substrate for Cellulase Production through Solid State Fermentation using Response Surface Methodology

Sobia Nazir¹, Muhammad Irfan¹*, Muhammad Nadeem², Hafiz Abdullah Shakir³*, Muhammad Khan³, Shaukat Ali⁴, Quratulain Syed²

... viride was used for cellulase production in solid state fermentation (SSF) using seed pods of Bombyx ceiba as substrate. Three parameters were used such as inoculum size (1, 5.5 and 10%), medium volume (1, 3 and 5ml) and incubation period (3, 4, 5 days) to observed the maximum activity of cellulase using Box-Bhenken design of response surface methodology. Maximum activity of CMCase (0.700 IU/gds/min) and FPase (0...

Cellulase Production by Trichoderma viride in Submerged Fermentation using Response Surface Methodology

Tehreem Zahra¹, Muhammad Irfan¹, Muhammad Nadeem², Misbah Ghazanfar¹, Qurratulain Ahmad³, Shaukat Ali⁴, Farzana Siddique⁵, Zarina Yasmeen⁶, Marcelo Franco⁷

...ichoderma viride for cellulase production using seed pods of silk cotton tree as substrate in submerged fermentation by response surface methodology has been investigated. Three variables like substrate concentration, peptone and KH₂PO₄were optimized at three levels. The optimum carboxymethylcellulase (CMCase) activity and filter paper cellulase (FPase) activity ...

Influence of Exogenous AHLs and Quorum Quenching AiiA Protein on the Production of Cellulase Enzyme in Marine Snow Associated Bacterium, Citrobacter freundii B1

Abdul Nabi Jatt^1,2*

...duction of extracellular cellulase enzyme. Mainly, two different reporter strains such as Agrobacterium tumefaciens A136 and Chromobacterium violaceum CV026 were applied to confirm the production of signal molecules. The identification of signal molecules was achieved by GC-M...

Evolutionary Adaptation of Fermenting Strains in Wheat Straw Hydrolysate for Bioethanol and Lactic Acid Production

Imrana Khushk¹, Abdul Nabi Jatt², Abdul Sattar Qureshi¹*, Choudhary Haider Ali³, Muhammad Aqeel Bhutto¹, Imran Ibrahim Khaskheli¹, Sartar Iqbal Panhwer¹

...t inhibitors and cost of cellulase enzyme). In the present study, long term evolutionary adaptation was evaluated for ethanol and lactic acid production to overcome the pretreatment inhibitors issue. Saccharomyces cerevisiae Angel was used for the production of ethanol, while, Bacillus coagulans, Pediococcus sp. and Lactobacillus sp. were used for lactic production, respectively. All the microbial strains were inoculated on wheat straw hydrolysate and were mon...

Improving Azolla microphylla through Fermentation with Lignocellulolytic Fungi and its Application in Broiler Feed

Nuraini Nuraini*, Mirzah Mirzah, Yuliaty Shafan Nur, Harnentis Harnentis

... The parameter measured: cellulase activity, crude fiber, fiber digestibility, crude protein, and nitrogen retention. The results of phase 1 showed that the types of fungi had a very significant effect (P <0.01) on the nutrient quality of fermented Azolla microphylla. Fermentation of Azolla microphylla with Lentinus edodes and Pleurotus ostreatus gave the best products. Phase 2. Application Fermented Azolla microphylla (AMF) in the diet on broiler performa...

Screening, Characterization and Purification of Cellulase from Mango Mealybug Drosicha stebbingi Green (Homoptera: Margarodidae)

Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf

Fermentation Characteristics, In Vitro Nutrient Digestibility, and Methane Production of Oil Palm Frond-Based Complete Feed Silage Treated with Cellulase

Budi Santoso*, Bambang Tjahyono Hariadi, Marlyn Nelce Lekitoo

...o assess the impact of a cellulase additive. In the complete randomized design experiment, six treatments (silage) were used: A, B, C, D, E, and F. Treatment A consisted of oil palm frond (40%), king grass (30%), cassava (10%), tofu waste (10%), molasses (7%), lactic acid bacteria (LAB) inoculant (3%), and cellulase 0 ml/kg. Treatment B comprised silage A + 3 ml cellulase/kg, while C used ...

Optimization of Cellulase Production by Chaetomium thermophilum in Submerged Fermentation using Wheat Straw as Substrate

Sabahat Anwar¹, Faria Javaid² and Qurat-ul-Ain²*

...d industrially important cellulase enzymes by using a thermophilic fungus i.e., Chaetomium thermophilum, which can efficiently produce cellulases extracellularly while growing on cellulosic substrates. Wheat straw, by product of wheat crop has been used as lignocellulosic substrate. To determine the optimum conditions for maximum production, experiment was carried out at various substrate concentrations, temperatures and pH ...

Enhancing Broiler Chick Growth with Locally Produced Thermostable Cellulase in Fibrous Diets

Aisha Khalid1,2, Roheela Yasmeen1*, Farah Ahmad1, Mudassar Aslam2, Muhammad Tayyab2

...recombinant thermostable cellulase from Thermotoga naphthophila (CELTN) on the growth performance of broiler chicks when added to corn-based feed. The thermostable cellulase was produced using BL21 CodonPlus cells with the pET28a-Cellu vector under optimized conditions. A total of 150 one-day-old, unsexed broiler chicks were divided into five treatment groups, each further subdivided into three replicates of 10 chicks with a...

Effects of Storage Time on the Chemical and Microbiological Quality of Pasteurized Beetroot (Beta vulgaris L.) Juice with Enzyme Extraction and Clarification

Abdussalam Muhyideen Zainab Funmilayo and Lee-Hoon Ho*

...ified with pectinase and cellulase and their combination, at concentration of 1.0 U/ mL for 50°C and 15 min. After that, the mixtures were all kept at 90°C for five minutes in order to pasteurize the juice. Beetroot juice without treatment: fresh juice and juice with enzyme extraction but not pasteurised nor clarified served as control 1 and control 2, respectively. The juices were evaluated for pH, total titratable acidity, total soluble solids, vitam...

The Distribution of Bacillus spp. in the Digestive Tract of Arab Chicken (Gallus turcicus) and the Evaluation as Indigenous Probiotic

Faturrahman^1,3*, N.L. Nakadira¹, N. Anjani¹, B.N. Fajriah¹, B.F. Suryadi¹, A.A.M.N. Kasman², T.W. Setyaningrum¹

...se, amylase, lipase, and cellulase enzymes. The distribution was measured by isolating, counting, characterizing, and identifying Bacillus spp. bacteria in five segments of the digestive tract. The isolates were tested for their ability to inhibit the growth of pathogenic bacteria using the paper disk method, as well as for their capacity to hydrolyze protein, amylum, lipids, and cellulose. Moreover, the degree of pathogen inhibition and macromolecule hydrolys...

New source of cellulase production using a metagenomic technique

Safaa M. Ali1*; Nadia A. Soliman2

Novel Research in Microbiology Journal (2019), 3(6): 590-597

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Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria

Alaa Fathalla Mohammed

Novel Research in Microbiology Journal (2020), 4(1): 641-652

...timize the production of cellulase and chitinase enzymes by plant growth promoting rhizobacteria (PGPR). Bacteria were pre-isolated from soil and identified as; Pseudomonas fluorescens NBRC (KW1), Serratia liquefaciens ATCC 27592 (EW1), Bacillus subtilis SBMP4 (EF1) and B. megaterium NBRC 15308 (TF2). The effect of different growth parameters including; pH, temperature, carbon and nitrogen sources, and incubation periods were optimized for production of

Endophytic bacterial communities colonizing the medicinal plant Calotropis procera: as resources of hydrolases

Fatma M. Abdel Baset; Noura Sh. A. Hagaggi*; Francis F. Hezayen; Usama M. Abdul- Raouf

Novel Research in Microbiology Journal (2020), 4(6): 1045-1056

...i.e., amylase, protease, cellulase, lipase and L-asparaginase. The aims of the current study were to identify the endophytic bacteria associated with the different organs of the medicinal plant C. procera, and to evaluate their potentialities to produce diverse extracellular hydrolytic enzymes.

...

Mevalonate pathway analysis of Saccharomyces cerevisiae during bioisoprene synthesis

Simiat O. Jimoh*; Semirah A. Sanusi; Farouq A. Olaogun; Ramot B. Badmos-Oladapo; Kifayat O. Asafa- Adedimeji

Novel Research in Microbiology Journal (2022), 6(6): 1821-1835

...zymes (e.g., amylase and cellulase), and mevalonate pathway enzymes, including isoprene synthase, mevalonate-5-diphosphate decarboxylase, and isopentyl phosphate kinase, in addition to isoprene, mevalonic acid (MVA), and its isomer dimethylallyl diphosphate (DMAPP). Based on the results obtained in this study, bioisoprene synthesis via direct fermentation of cheap and abundant carbon sources such as cassava peel using the S. cerevisiae 19KB strain will overcom...

Use of rpoB gene phylogenetic marker-based distinction of abiotic stress tolerant and plant-growth promoting Bacillus paralicheniformis isolates from their closely related Bacillus licheniformis

Mohammed Ajdig1,2; Bahia Rached2,3; Ahlam Mbarki1,2; Taha Chouati2,4; Chouhra Talbi1; Elmostafa El Fahime2,4; Marouane Melloul1,2*

Novel Research in Microbiology Journal (2024), 8(3): 2414-2434

...tivities (i.e., lipases, cellulases, and proteases). In addition, several PGP traits such as phosphate solubilization and the production of siderophores and indol-3 acetic acid were also observed. Molecular identification through 16S rRNA sequencing initially identified all the isolates as Bacillus spp. The multi-locus sequence typing (MLST) scheme, using six housekeeping genes (adk, ccpA, recF, rpoB, sucC, and spo0A) had unveiled distinct allelic profiles res...

Effect of Local Microorganisms from Bali Cattle Rumen Fluid and Palmyra Sap on Fiber Fractions and Nutrient Composition of Fermented Rice Bran

Tri Anggarini Yuniwati Foenay¹, Theresia Nur Indah Koni¹*, Agustinus Paga¹, Oswilda Henyati¹, Yosefus Frederikus da-Lopez²

... rumen fluid can produce cellulase enzymes that degrade crude fiber, while palmyra sap can reduce phytic acid levels in rice bran. In this study, local microorganisms (MOL NR) were sourced from a mixture of rumen fluid, palmyra sap, and sugar water, fermented for three days. This study aimed to examine the effect of different MOL NR levels on the nutritional composition and fibre fraction of rice bran fermented for six days. The study used a completely randomi...

Acid-Producing Enterococcus Sp. from Grati Lake’s Endemic Gobiopterus Sp.: A Novel Probiotic Candidate

Mikka Hakkinen¹, Asep A. Prihanto²*, Abdul R. Faqih³, Septi Anitasari³, Happy Nursyam², Royani L. Hayati⁴, Hefti S. Yufidasari²

...oduction of protease and cellulase enzymes, aiding in nutrient digestion. DNA sequencing identified four isolates as belonging to the genus Enterococcus, a well-established probiotic genus. These findings suggest that Enterococcus spp., identified as a lactic acid bacteria isolated from Gobiopterus sp., is a promising candidate for use as a probiotic.

Keywords | Biofilm, Enzymes, Gobiopterus sp., Grati lake, Lempuk fish, Probioti...

The Evaluation of Tea Extracted from the Dried Roots of Asparagus officinalis L. using Cellulase

Nguyen Thi Ngoc Giang¹* and Tran Van Khai²

...ragus roots by utilizing cellulase. The investigation examined cellulase enzyme concentrations at 1, 2, 3, and 4% (v/w, compared with raw material) and pH levels at 4.5, 5.0, 5.5 and 6.0 to evaluate the quality of the extracted tea solution. Parameters of pH 5.5 and 3% enzyme were selected as optimal condition for the extraction. The extract from dried asparagus roots derived under this condition demonstrated significant nut...

Evaluation of Fermentation Quality and In vitro Nutrient Digestibility of Oil Palm Fronds-Based Complete Silage with Various Additive Treatments

Budi Santoso*, Evi Warintan Saragih, Bambang Tjahyono Hariadi

...t of adding additives of cellulase and xylanase enzymes, urea, as well as Ca(OH)2 to complete silage based on oil palm fronds on chemical composition, fermentation characteristics, and in vitro nutrient digestibility. A completely randomized design with six treatments and three replicates was chosen in the experiment. The six complete silage treatments included: A: 40% Oil palm fronds, 30% king grass, 10% cassava waste, 10% tofu waste, 7% molasses, and 3% Lact...