El-Sayed M. Abdelwhab, Jutta Veits and Thomas C. Mettenleiter
Avian Influenza H5N1 in Egypt: What we Know and What we have to Know?
...demic outbreaks of avian influenza viruses (AIV) of subtype H5N1 in poultry since 2008. Since then, these viruses continue to evolve. In 2006-2008, outbreaks were reported mostly during the winter season, but in 2009-2012 they occurred year round. H5N1 AIV succeeded in infecting not only domesticated birds but also many other avian species and mammals. Infections of humans were accompanied by mutations in the hemagglutinin (HA) protein which improved the bindi...
Sandeep Satapathy and Rupam Ghosh
...diators for clearance of influenza by up regulating several intracellular defense mechanisms. Associated with IL-6 signalling is the expression acceleration of sequestrome 1(SQSTM1) / aggrosome and colocalization of HDAC6 to the cytoplasm. The key player HDAC6 is investigated for modelling several intracellular processes that come into action to bring out the viral clearance. The shuttling of HDAC6 takes place from nucleus to cytoplasm and vice versa controlli...
Hanqin Shen1,3, Boliang Wu1,2, Guangwei Li1, Feng Chen1, Qingbin Luo1,3, Yimin Chen1, Qingmei Xie1,2,3*
...p>The H9N2 subtype avian influenza virus (H9N2 AIV), which has been in circulation for two decades, has become the predominant subtype in China, attracting considerable attention due to human infections and the contribution of internal genes to H5N1 and H7N9 influenza viruses. Multiple H9N2 AIV genotypes have been identified from various species, including birds and mammals. H9N2 AIVs could spread through water, air, and liv...
Weili Kong1, Guangpeng Ma2 and Jinhua Liu1*
...erating the virulence of influenza virus by multiple mechanisms. Due to C-terminal ‘tail’ (CTT) truncation of NS1, there are length variation types of NS1 in different subtype influenza viruses. CTT functions in several ways to defeat the cellular innate immune responses. Here, we discuss those different effects of CTT truncation or elongation of NS1 protein in different genetic backgrounds of
Suresh V Kuchipudi* and Kin-Chow Chang
...roductive replication of influenza A virus (IAV) and could play an important role in disease outcome. We are of the opinion that skeletal muscle could be a key innate immune tissue/organ in the pathogenesis of influenza and other systemic viral infections which warrants further in-depth investigations.
...
Leonard Izzard1, 2, John Stambas1, 2*
...iRNA-based technology in influenza research is rapidly expanding. miRNAs are currently being used to manipulate pathogenicity and immunity and as biomarkers for disease severity....
Josef D. Jarhult1, 2
...ros and cons of the anti-influenza drug oseltamivir (Tamiflu®) have been intensely debated lately. This article aims to sum up what is known about positive and negative effects of the drug. The available data suggests that oseltamivir treatment of uncomplicated influenza in otherwise healthy patients shortens influenza symptoms with approximately 24 hours, and increases nausea and vomi...
Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3
Email: mohammedvet1986@gmail.com; mohammed_abdelmohsen@cu.edu.eg
...ses including avian influenza (AI). Biosecurity measures, controlling poultry movements and inactivated vaccines were devised to combat the spread of highly pathogenic avian influenza (HPAIV) H5N1. HPAIV are highly susceptible to all disinfectants because they are enveloped viruses. Disinfection against avian influenza viruses at the poultry farms would significantly re...
Shubhada K Chothe, Bhushan M Jayarao and Suresh V Kuchipudi
...t the role of lncRNAs in influenza virus infection and the exciting prospect of exploiting lncRNAs as targets to develop novel anti-viral therapies....
Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2
...body titres against H9N2 influenza viruses and its molecular detections in asymptomatic commercial layers from Faisalabad district of Punjab, Pakistan. Overall 120 blood samples, 24 tissue samples from each organ (trachea, lung and intestine) were collected from the 12 commercial layer flocks selected randomly at the age of 35-50 weeks without any direct clinical manifestation of avian influenza. Serum collected from 120 bir...
Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan
...-infections....
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...The low pathogenic avian influenza viruses (LPAIV) subtype H9N2 are highly prevalent in the Middle East and are causing economic losses in the commercial poultry production sectors. Due to potential of H9N2 infections in human, LPAI H9N2 poses serious public health risk. Therefore, development of new potent and cost-effective influenza vaccines is urgently needed to safeguard poultry and to contain zoonosis. The primary goal...
Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2
...se virus (NDV) and avian influenza viruses (AIVs) H5N1 are endemic in Egypt and extensive vaccinations are being applied for chicken flocks, which have maternal derived antibodies (MDA) for these viruses. To understand the interference of maternal derived immunity impact of maternal derived immunity interference in vaccination regimen in poultry, a total of one hundred day old commercial broiler chicks were vaccinated with LaSota. A group of chicks (n=40) were...
Muhammad Ayaz1,*, Muhammad Athar Abbas2, Pervez3, Noorulain3, Yasir Amin1, Zubair Ali3, Naila Siddique2 and Khalid Naeem2
...>A surveillance of avian influenza viruses in Hazara region of Khyber Pakhtunkhwa Pakistan was carried out from April 2013 through September 2014. Pooled morbid material and fecal samples from 600 different locations across the region were analyzed for isolation. Isolation was carried out on embryonated chicken eggs. Identification of H protein was undertaken through hem agglutination Inhibition (HI) test while N typing was done using reverse transcriptase pol...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
... highly pathogenic avian influenza virus (HPAIV) strain H5N1 in 2006 in Egypt caused severe economic losses for both commercial and backyard poultry production sectors. Owing to public health risk, effective control of the virus in imperative. DNA vaccination is a promising new approach for the prevention and treatment of many viral diseases because of its ability to induce both humoral and cellular immune responses against antigens encoded by recombinant DNA....
Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4
...stify;"> H9N2 avian influenza viruses are currently endemic in Pakistan and outbreaks are continuously being reported with high morbidity, drop in egg production and reduced weight gain. Besides economic losses to the poultry industry, these viruses pose a significant threat to public health. Owing to significant involvements of neuraminidase (NA) gene in virus pathobiology by mediating virus entry and release, the present study was conducted to determine...
Mohsan Ullah Goraya1,2, Liaqat Ali1,2 and Iqra Younis3
...ratory viruses including influenza virus, coronaviruses and herpes viruses are important pathogens that cause significant losses in the poultry industry. However, successful establishment of infection in the respiratory tract switch on the pathogen recognition by innate immune receptors and establish first line of defense against viral infections. When specific cellular receptors recognize a virus they trigger the translation of inflammatory cytokine and chemo...
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
...nsive epidemic of equine influenza (EI) in October 2015 in equine population. EI is among the OIE or WHO notifiable, contagious equine respiratory diseases. Due to lack of awareness and interest of all the equine industry stakeholders no vaccination against EI is performed, also the information regarding the epidemiology and occurrence of EI is lacking in Pakistan. We hereby attempted to determine the seroprevalence, investigating the EI outbreak 2015-16, and ...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
Evolutionary Analysis and Phylodynamics of Avian Influenza Virus H5N1 between 2015 and 2016 in Egypt
Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*
...ortance.
...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
... the prevalence of avian influenza virus (AIV) subtypes (AIV H9, AIV H7 and AIV H5) in non-vaccinated wild and domesticated bird species across Pakistan. During January-December 2013, in total 700 samples were collected from different species of non-vaccinated birds inhabiting diverse ecological zones of Pakistan. Altogether, 507 tissue and swab samples were screened for the presence of viral RNA by reverse transcriptase polymerase chain reaction (RT-PCR). Sam...
Ali Zaman1,*, Abdul Haleem1, Sajjad Ur Rahman2, Adnan Amin3 Asma Saeed1, Sana Ullah4 and Naimat Ullah4
...hygiene.
... 
Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1
Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
...ction....
Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1
...V and negative for avian influenza virus and infectious bronchitis virus. Seven positive samples were subjected for genotypic characterization based upon partial F gene sequencing. Partial F gene sequencing revealed that all tested isolates harbor a cleavage site with multi basic amino acid 112KRQKR/F117 characteristic of velogenic strains. However, phylogenetic analysis revealed clustering of all tested strains within sub-genotype VIg. Three isolates were ass...
SiRui Wang1,2, Fekede Regasa Joka1,2, XiaoLong Wang1,2,* and SuYing Bai2,*
...n and treatment of avian influenza. Currently, this gene is more studied in poultry, and studies in wild birds are rare. To understand the antiviral site of the Myxovirus-resistance (Mx) protein in the evolution of different wild birds, 10 wild bird species, including Anas formosa, Anas crecca, Anas strepera, Mergus squamatus, Accipiter nisus, Buteo hemilasius, Buteo lagopus, Passer montanus, Psittacul...
Amany Adel, Wesam Mady*, Zienab Mosad, Fatema Amer, Asmaa Shaaban, Dalia Said, Marwa Ali Abdel Maged, Abdel-Satar Arafa, Mohamed Kamal Morsi, Mohamed Khalifa Hassan
...9N2 low pathogenic avian influenza virus in 2011, the virus has distributed rapidly and widely in different poultry sectors in Egypt causing severe economic losses and the problematic situation in poultry production especially with a co-infection with other circulating pathogens. In this study, 1026 confirmed positive H9N2 cases by RT-PCR out of 23182 different examined samples of different species and sectors in Egypt, with a prevalence rate 4.4% by the year ...
Amany Adel, Wesam Mady*, Zienab Mosaad, Fatma Amer, Asmaa Shaaban, Dalia Said, Marwa Ali, Abdel-Satar Arafa, Mohamed Kamal Morsi and Mohamed Khalifa Hassan
...9N2 low pathogenic avian influenza virus (LPAI) in 2011, the virus has been distributed rapidly and widely in different Egyptian poultry sectors causing severe economic losses and the problematic situation in poultry production especially with a co-infection with other circulating pathogens. In this study, a total of 23182 cloacal and tracheal samples were collected from suspected cases between 2015 and 2016 and submitted to the Reference laboratory of vete...
Wesam Mady*, Nahed Yehia, Mohammed Hassan Elhussieny, Azhar Gaber Shalaby, Moataz Mohamed El Sayed, Neveen Rabie Bakry, Asmaa Shaaban, Abdel Satar Arafa, Afaf Abdel Baset Mahmoud and Wafaa Mohammed Hassan
... highly pathogenic avian influenza virus (HPAIV) H5N8 in November 2016 in Egypt, it caused severe economic losses for both the commercial and backyard poultry production sectors. The aim of this work is to study the Situation and molecular characterization of HPAIV (H5N8) in backyard poultry production sector in Egypt. In this study, a total of 7505 samples of tracheal swabs representing 1180 backyard poultry flocks were collected based on the surveillance con...
Irfan Irshad1, Asim Aslam1,*, Muhammad Yasin Tipu1, Kamran Ashraf2, Beenish Zahid3 and Abdul Wajid4
...ow-pathogenic H9N2 avian influenza viruses are presently endemic in Pakistan and repeated outbreaks are continuously being reported with high mortality in poultry and non-poultry avian species. In this study, five AAvV-1s have been pathotyped and genetically characterized from vaccinated birds collected between 2013-14. A phylogenetic analysis revealed that all the isolates belonged to sub-genotype VIIi with high similarity of 97.9 to 99.8% with similar viruse...
Muhammad Naeem Khan* and Asad Jan
...ments like asthma, cold, influenza, head ach, liver and digestive problems and healing of wounds. The aim of the study was to explore phytochemical constituents of methanolic crude extract (MCE) and various solvent fractions and in-vitro antimicrobial activities of whole plant of D. botrys. Qualitative phytochemical screening of MCE and solvent fractions of D. botrys showed the presence of alkaloids, phenols, flavonoids, saponins, tannins and sterols, however ...
Muhammad Furqan Shahid1*, Tahir Yaqub1, Muhammad Yasin Tipu2, Asim Aslam2, Saima Yaqub1, Aziz-ul-Rahman3, Muzaffar Ali1
...rly low pathogenic avian influenza virus (LPAIV) H9N2, which can bring exceptional abatement in trade of poultry products in endemic countries such as Pakistan. The current study was conducted to evaluate the infectious and antigenic potential of three different AIV (A/H9N2) variants isolated from commercial, backyard poultry and fancy birds (partridge). Commercial broiler birds were infected experimentally at the dose of 106 EID50 by usi...
Khaled Kaboudi*
...y;">Low pathogenic avian influenza H9N2 subtype virus is considered among important respiratory pathogens in poultry worldwide. It was isolated from many species of domestic and wild birds. Infection in commercial poultry flocks causes important economic losses, related to high mortality, decrease in animal performance and aggravation of infection by other pathogens. Although H9N2 is endemic in many regions of the world, such as Middle East and North Africa, w...
Hamoudi Naoual1, Alloui Nadir2*, Barberis Abdelhak2 and Boudaoud Amine2
...irst source of the avian influenza virus (AIV) introduction. In 2016, following the first official detection of the H9N2 subtype circulation in broiler flocks in Eastern Algeria, a preliminary descriptive study is conducted to identify and enumerate in wetland areas, migratory bird species, known to carry the AIV. The Anatidae population peaked in January 2017 with a total of 8064 birds. The 6 frequent species observed are: Mallard (Anas platyrhynchos), Eurasi...
Christian Marco Hadi Nugroho1 and Jeanne Adiwinata Pawitan2,3,4*
Production of Antibody for Direct Fluorescence Antibody Assay against Avian Influenza H9N2
...lign: justify;">The H9N2 influenza A viruses are endemic in some countries for many years and had caused economic losses in poultry industries. Infection with this virus decreases egg production and increases chicken mortality. In human health, cases of this zoonotic agent are being reported and some cases showed fatal infection. The H9N2 is highly contagious, and transmission can occur from chicken to chicken, chicken to humans through the air, or sometimes f...
Ye Ge1,2,3, Qiucheng Yao1, Hongliang Chai3, Yuping Hua3* and Guohua Deng2*
...es, play active roles in influenza virus transmission and reassortment. To further evaluate the potential infection risk of H6 AIVs from wild birds, we inoculated these viruses into A549 cells, which showed that these wild bird H6 AIVs have acquired the ability to cross interspecific barriers and have the potential to infect mammals. We analysed the level of cytokine expression in response to four H6 AIVs in CEFs and DEFs using real-time PCR. The results showe...
Muhammad Akram Muneer1, Khalid Munir1, Ghulam Abbas1’*, Isra Munir1, Mumtaz Ahmad Khan1, Asif Iqbal1, Munsoor ud Din Ahmad1, Muhammad Arshad Javid2, Zareen Fatima1 and Maria Arshad1
...ry Syndrome (SARS), H1N1 influenza and Middle EastRespiratorySyndromecoronavirus(MERS-CoV)emerged as very importantissuesduringlastthree decadesas these infectionscaused quitelargenumberofhumandeathsworldwide.Coronavirusesaresingle-strandedpositive sense RNA viruses which mainly in past were considered responsible for high percentage of (around 30%) of common cold/flu cases. Viruses causing SARS, MERS and COVID-19 are members of family Coronavirdae. Wor...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Ying Wang1, Ding Zhang1, Yu-hai Bi1,2, Ali Raza Jahejo1,Qian-qian Cheng1 and Wen-xia Tian1,*
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...>Highly pathogenic avian influenza (HPAI) H5N1 virusesare now endemic in Egypt inducing economic losses in poultry industry and subsequent risk of human infection. Vaccination is an effective method for controlling avian influenza virus infections.This study was conducted to evaluate the efficacy of the commercially applied AI vaccine regimens against challenge with three H5N1 HPAI viruses isolated from chickens, Egypt durin...
Walaa Abd El-Fatah S. Metwally1, Ismail M. Reda2, Ahmed A. El-Sanousi2, Mohamed Abd El-Khalek Ali2
... the present study avian influenza (AI) inactivated vaccine was prepared with Montanide ISA70VG which has been added as adjuvant using reassortant AI strain H5N1. The prepared vaccines with Montanide ISA70VG varied in the HA units per dose and also differed in the ratios between two reassortant strains (Chicken and duck strains) of AI. The results of testing humoral immunity revealed that the prepared vaccines with Montanide ISA70VG containing 350 HA unit/dose...
Serag eldeen Sultana, M.W Abdel azeemb, Nahla Mohamed Eldamranyc
...te the presence of avian influenza virus (AIV) antibodies. The migratory birds were classified into (8) orders, (16) families and (23) species. The serum samples were examined using IDEXX MultiS-screen AI Antibody ELISA kit coated with AIV nucleoprotein and hemagglutination inhibition (HI) test against H5 and H9 antigens. The overall results using both ELISA and HI tests revealed that the prevalence of AIV antibodies was 12.6% (18/143). The seropositive sample...
Hanan M. El-Zahed, Hanaa A. Mostafa, Nermeen M. El Sayed and Lamiaa M. Omar
...en the inactivated avian influenza (AI) H5N1 vaccine was inoculated intra-muscularly either at the 1st, 2nd or 3rd WPV with CAV vaccine forming three subgroups per each group. It is clearly observed that a relatively high levels of CAV ELISA antibody titers in all the vaccinated chicken subgroups at 4th WPV with either DW or S/C CAV vaccine indicating that the two CAV vaccines were immunogenic; Also, by measuring H5N1 HI titers within the 6 weeks post vaccinat...
Amany Adel1, Abdelsatar Arafa1, Hussein A. Hussein2 and Ahmed El-Sanousi 2
Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden
...v>...
Maha M. Khedr1, Reem A. Suliman1, Marwa F. Mohamed1, Mounir D. El Safty1, Hussein A.
Hussein2
...accination against avian influenza in early age in chicks.
Methods: Eight groups of one-day old commercial broiler chicks were kept in isolators along the whole
period of the study. Groups 1, 2 and 3 were vaccinated with the prepared vaccine; group 4, 5 and 6 were
vaccinated with one of the imported reassortant H5N1 containing 300 HAU at 1, 5 and 10 days of age;
respectively. Groups 7 and 8 were positive and nega...
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
...antigen content of avian influenza virus
vaccines is urgent to reach protective antibody titers and reduce virus shedding.
Methods: Groups of one-day old commercial broiler chicks were divided in to 8 groups 1, 2 and 3 were
vaccinated with a prepared vaccine contain 500HAU of H5N1 reassortant antigen; while group 4, 5 and 6
were vaccinated with an imported reassortant vaccine with 500HAU antigen content of H5N1 a...
Dalia M. Omar1, Nermeen A. Marden1, Lamiaa M. Gaafar1, Elham A. El-ebiary1, Khalid El-Dougdoug2, Badawi Othman2, Abd Elsattar Arafa3, Hussein A. Hussein4
...v>...
Nermeen A. Marden1, Lamiaa M. Gaafar1, Hussein A. Hussein2
Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2
...T) and
avian influenza (AI) that affect their populations in a dramatic form. Successful control of these viral
diseases depends on the implementation of well-designed vaccination programs using highly potent
and safe vaccines.
Aim: The present study investigated the effect of simultaneous administration of inactivated AI
vaccine and live attenuated TRT vaccine on the immune response of turkey chicken...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer and Ahmed A. El-Sanousi
...g the existence of avian influenza subtype H9N2 (AI
H9N2) in 2006 in Lebanon; a lot of pathological changes and mortalities were seen among chicken
flocks suggesting the influence of the AI H9N2 virus.
Objective: this study reports the circulation of H9N2 associated with mortalities in broiler sector and
decrease egg production in layer hens and breeders in Lebanon.
Methods: A total of 22 poultry floc...
Mohamed F. ElKersh1, Fatma M. Abdalla2, Gamilat K. Farg2, Soad A. Nasef3, Ahmed A. Ali2
...cular Diagnosis of Avian influenza virus (AIV) from Different Avian Species that would be useful to detect the current strains present in Egypt to use it in vaccination program. Methods:In this study, we collected 122 sample representing 12 different area in Egypt and seven different species of poultry produced and traded in Egypt. These samples were isolated on SPF eggs, detected for AIV infection Via HA, RT-PCR and gene sequencing. We analyzed the sequence o...
Hanan Sayed1, Mohamed S. Madkour1, Hussein A. Hussein2, Mounir Mohamed Elsaft 3,
Marwa F. El saied3, Ahmed A El-Sanousi2
Hanan Sayed 1, Mohamed S. Madkour 1, Hussein A. Hussein 2, Ahmed A El-Sanousi 2
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Qian-qian Cheng1,
Raza Ali Mangi1, Ali Raza Jahejo1, Afrasyab Khan1, Yu-hai Bi1,2 and Wen-xia Tian1*
...font-size: small;">Avian influenza virus (H9N2) is one of the major cause of illness in chickens with potential threats to human health. Chicken erythrocytes are known as most abundant circulating cells in the body which participate in inflammation and immune responses. Influenza viruses interact with the host cells at cell surface, leading to cell death in human and animals through apoptosis. Current study was designed to i...
Rehman Shahzad1, Saba Irshad1* and Faisal Amin2
...t-align: justify;">Avian influenza A virus subtype H9N2 has been in circulation since last two decades in poultry industry of Pakistan. This virus is causing huge economic loss every year due to poor weight gain, drop in egg production and mortality of birds since first reported in 1998. Influenza viral surface protein neuraminidase enhances both virus replication and its release from host cells. In this study we isolated ni...
Rehman Shahzad1, Saba Irshad1*, Malik Saddique Mehmood1 and Faisal Amin2
...t-align: justify;">Avian influenza A virus subtype H9N2 has been in circulation since last two decades in poultry flocks of Pakistan. It is causing immense economic losses to the farmers. Avian influenza H9N2 virus were isolated from infected birds of different poultry farms in the Province of Punjab, Pakistan. Segment eight gene of the virus was amplified using RT-PCR and sequenced to analyze mutations in this viral segment...
Amjad Ali Channa1, Mansoor Tariq1*, Zaheer Ahmed Nizamani1, Nazeer Hussain Kalhoro2
...arachi ...
Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*
...ther avian viruses avian influenza (AI), new castle disease virus (NDV) and infectious bursal disease virus (IBDV) were not affected by enzymatic inductions. Therefore, the antigen prepared by phospholipase C has been found to be more effective for HI test for rapid diagnosis of IBV during infection....
Sana Shakoor, Tahir Rehman Samiullah, Naila Shahid, Abdul Qayyum Rao*, Aneela Yasmeen, Sana Tahir, Ayesha Latif, Saira Azam, Ahmad Ali Shahid and Tayyab Husnain
...H9N2 strain of the avian influenza virus has emerged to infect poultry during the last few years. Besides antiviral treatment development of an effective vaccine to combat a high mortality rate is the need of time. As a step towards the development of the vaccine for the H9N2 strain of avian influenza virus HA gene was chosen as the immunogenic antigen because it is highly conserved among H9N2 strain of avian
Md. Taimur Islam1*, Mirza Mienur Meher2, Anas Bin Harun3 and Md. Golam Haider1
...ion in Bangladesh. Avian influenza (AI), Newcastle disease (ND), infectious bronchitis (IB), infectious laryngotracheitis (ILT), Avian Metapneumovirus (AMPV), infectious coryza, fowl cholera and aspergillosis are the most prevalent respiratory diseases in Bangladesh. These diseases are most prevalent in layer, broiler, sonali and backyard chicken. AI outbreak is observed every year since its first report in 2007 in Bangladesh. This virus has mutated several ti...
Anis Suraya Mohamad Abir1, Sakinah Yusof1, Abd Rahaman Yasmin1,2, Abdul Rahman Omar1,3, Kok Lian Ho4, Wen Siang Tan1,5, Abdul Razak Mariatulqabtiah1,6*
...ing the H5 gene of avian influenza virus (rFWPV-H5) using morphological and immunophenotypical observations. The results showed that the rFWPV-H5-infected chBM-DCs formed better dendrites and stellate cells, compared to the WT FWPV. The recombinant virus also expressed higher levels of the surface markers CD86 and MHC class II double-positive chBM-DCs 12 and 24 h post-infection. In conclusion, chBM-DCs were susceptible to WT FWPV and rFWPV-H5 infections, where...
Abdelrahman A. Abdelrahman1, Salama A. S. Shany1, Kareem E. Hassan1, Mansy A. A. Dardeer2, Ali A.1*, Magdy F. El-Kady1*
...the prevalences of avian influenza virus (AIV), infectious bronchitis virus (IBV), and Mycoplasma spp. are high. Thus, co-infection with one or both viruses and Mycoplasma gallisepticum (MG) or Mycoplasma synovaie (MS) is thought to play an important role in the clinical severity of respiratory infections. In this study, we conducted a passive surveillance study for MG and MS among cases of viral respiratory affections in broilers. Samples were collected from ...
Hany M.R. Elsherif1, Ahmed Orabi2, Hussein M.A. Hassan3, Ahmed Samy3*
....05) via enhancing avian influenza (H5) and Newcastle disease (ND) titers. Accordingly, OAS’s as natural feed additives could improve broiler performance and immunological state. Chicks fed SF or SP had the best productive performance, immunological status, digestive parameters, and microbiota.
Keywords | Blood parameters, Broiler chicks, Gut health, Immune status, Organic acids salts, Productive performance
...
Mohamed. E. Taha1, Mohamed S. Ahmed2, Ahmed I. Ahmed2, Amany Adel3, Salama Rehab4,5*, Nabila Osman2**
...ncing. ...
Effects of Antibiotics on Growth Performance, Immune Response, and Intestinal Microflora of Broilers
Shahan Azeem1*, Muhammad Akram Muneer2, Liaquat Ahmad3, Sameera Akhtar1 and Talat Naseer Pasha4,5
...se virus (NDV) and avian influenza virus (AIV) antibody response, intestinal microflora, and feed efficiency of broilers. Forty-five day-old broiler chickens were randomly divided into five treatments and housed under identical husbandry conditions. Antibiotic-free poultry feed was procured from a commercial feed mill. The antibiotics were mixed in the feed as per the manufacturer’s instructions and offered ad libitum to the chickens for the entire study...
Muhammad Tariq Navid1,2*, Mian Muhammad Awais2*, Muhammad Irfan Anwar2 and Masood Akhtar2
...t-align: justify;">Avian influenza viruses (AIVs) are the serious health concern throughout the globe and induce heavy economic losses in poultry industry. In the rural settings, the domestic birds are neglected for avian influenza (AI) vaccination while they are directly connected with migratory carrier birds throughout their life. Keeping in view this study was design. Four Tehsils of district Multan were selected to inves...
Sara Magdy Hashim1, Elshaimaa Ismael2, Mohamed Tarek3, Faten Fathy Mohammed1*, Fatma Amer Abdel Reheem3, Rawhia Esawy Doghaim1
... highly pathogenic avian influenza (HPAI) H5N8 virus causes severe economic deficit in the poultry production in Egyptian farms and has a potential intimidate for the public health, this subtype firstly was detected in Egypt in 2016 from wild birds. In the present study, samples were collected from 100 Egyptian domestic ducks of different ages from seven Egyptian governorates from period 2018 to 2020. Tissue specimens were allocated for HPAI H5N8 diagnosis usi...
Carla Meneguin Barbosa1*, Fabio Gregori2, Cairo Monteiro1, Thais Helena Martins Gamon1, Luciano Matsumiya Thomazelli1, Tânia de Freitas Raso2 and Edison Luiz Durigon1
Asmaa A. M. Abdel-Ghany1, Ahmed N. El Taweel2, Yassmin Moatasim2, Nagwa S. Ata1, Amany Adel3, Ayman Hany El-Deeb4, Ahmed Kandeil2, Mohamed A. Ali2*, Hussein Ali Hussein4*
...>Highly pathogenic avian influenza (HPAI) H5N8 virus of clade 2.3.4.4 was first emerged in China. The virus disseminated throughout migrating birds into different countries in Asia, America, Europe and Africa. HPAI A/H5N8 viruses were initially reported in Egypt in winter of 2016. However, backyard poultry production sector is commonly found in rural areas in Egypt with biosecurity deficiencies and could play a key role in epidemiology of AIVs in Egypt. Thus, ...
Xiaoyan Yang1* and Xiuli Xu2
...as performed to evaluate influenza-like virus effects in adults. This case-control study was performed on 50 patients admitted to the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine during the autumn and winter seasons with myocardial infarction and 50 patients as controls. History, tests, and clinical examinations in both groups did not show diabetes, hypertension, dyslipidemia, or smoking. For both groups, ...
Lianci He1, Dingxi Bai2, Chenxi Wu2, Yizhu Zhong2, Shi Chen2, Xinru Bao2, Jing Gao2*, Chaoming Hou2*
...ine hydrochloride group (influenza group, FLU) and chronic fatigue syndrome (CFS) group. In addition to the CON group, the remaining five groups established CFS rat models through forced swimming test and chronic restraint stress. After 28 days, the three groups received different concentrations of SXC (1.62 g/ml, 0.81 g/ml and 0.41 g/ml), FLU group received 0.21 mg/ml fluoxetine. CFS group and CON group received the same volume of normal saline. All groups re...
Olabode, H.O.K1, Mailafia, S1, Martha Echioda-Ogbole1*, Ameh, J.A1, Amadi, K.I1 and Meseko C.A2
Ibrahim l , Madiha Salah and Ikuta 2, Kazuyoshi
...apid on-spot testing for influenza virus can greatly assist in the clinical management decision. In Egypt, where avian influenza A virus H5Nl is now endemic and human infections are on the rise, rapid H5N1 detection in infected poultry is essential for controlling spread to human. To test the performance of two rapid influenza tests for the detection in naturally infected birds, clinical s...
Hagag, N.M.*•, Arafa, A.*; Shalaby, M. A. ** El-Sanousi, A. A. **and Aly, M. M.
...>Highly pathogenic avian influenza (HPAI) caused by influenza A H5N1 virus, poses a significant threat to the poultry industry and humans in Egypt. Since it was first recognized in 2006, the disease has become enzootic in poultry throughout Egypt and still circulates in the poultry population, so the ability to rapidly recognize AlVs in biological specimens is critical for limiting further spread of the disease in poultry. A...
Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.
...d for detection of avian influenza (Al) isolates using rapid haemagglutination test (HA). It was found that, all the inoculated isolates caused high mortalities up to 100% for the embryos 24-48hrs post inoculation and gave very strong RBCs agglutination. The haemagglutination positive samples were identified and subtyped antigenically by serological tests using Haemagglutination inhibition test (HI) using standard Al antisera and genetically by RT-PCR using sp...
Abd El-Razakl, A.G. and AboElkhair2, M.
...castle disease and avian influenza vaccines. It was also verified by an experimental very virulent IBD challenge performed at the age of 2e day in birds transferred from the farms with appropriate control groups in a laboratory. The immune response on farm A showing higher serological titer at the age of 35 day via ELISA test. Both two vaccines provide complete protection against mortality but not protection against bursal atrophy or histopathological changes ...
Ebied, Eman M.; Salem, Zeinab T. and Al-Imam, Hemmat S.
...ated freeze-dried equine influenza (El) vaccine, reconstituted in diethylaminoethyle (DEAE) Dextran solution as an adjuvant. It was found that mares developed high haemagglutinating inhibition (HI) titre at 3 weeks post vaccination and remain with protective level up to 6 months, while their foals had moderate (HI) antibodies within 48 hours post colostral suckling, the HI antibodies rise to a level similar to their dams within one to two weeks then pers...
Arafa, A ; El-Kanawaty, Z; Hassan, M.K; Anwar, H.K and Mona M.Aly
... highly pathogenic avian influenza (HPAI) virus Of subtype H5N1 affected all commercial poultry sectors as well as rural and backyard level in Egypt. This outbreak also was extended to include the zoo birds In Giza zoo. This study records the isolation and characterization of H5Nl virus from different species of the zoo birds and also studies the immune states of the vaccinated birds after application of H5Nl vaccine for the first time one month after the intr...
Hussein, HA; sultan, HA. Al Deeb AH and AA. El-Sanousi
...rent H5Nl and H5N2 avian influenza vaccines (Reassortant H5N1 and 6 types of H5N2 vaccines) by hemaggtutination inhibition (HI) test using heterologous H5 antigen which represent non of the vaccine antigens. The samples were collected from 578 houses mostly commercial layers. H5Nl viral nucleic acid was also detected in swab samples collected from mortalities occurred in some of vaccinated birds after vaccination policy has been applied in Egypt at March 2006....
Madbouly, H.M; Orkhan, M.H**; Nagwa El-khoty
...t-align: justify;">Avian influenza outbreak was firstly appeared in poultry reared in backyards or roofs of homes in Qaluobia & Giza governorates as many notifications were received by the General Organization of Veterinary Services (GOVSs) in February 2006. The disease was spread and recorded in chickens (foreign breeds , Balady , and hybrids ) , turkeys, ducks , geese and wild birds (from Giza Zoo) in 20 out of 26 (76.9 %) of the Egyptian governorates wi...
Madbouly, H.M; Orkhan, M.H**; Nagwa El-khoty
...>Highly pathogenic avian influenza (Al) H5N1 strains have been isolated during February 2006 from infected chicken located at El-Qanater El-Khiria center, Qaluobia, Eypt. These isolates were molecularly characterized and compared with those H5Nl strains isolated during January 2007 from the same locality. The strains isolated from infected chicken during 2006 were closely related to A/ty/Turkey/l/05 strain which had been isolated from turkeys in Turkey 20...
Abd Elwanis, N.D.; Abou El Khair, M.A.; Afaf H. Amin; Azab, A. and A.O. Abd El Rahman
...the low pathogenic avian influenza (LPAI) virus A/Turky/CA/209092/02, H5N2 for the first time in Egypt. Virus strain from which the vaccine seed virus has been prepared was derived from the USDA, Ames Iowa, USA. The specific characters of this strain were studied; It was found that the best virus dilution of Al virus for egg inoculation was 10-3 to 10-4, the peak virus titer was obtained 72 hours post inoculation, the predilection sites of the virus multiplica...
Arafa, A.; Kanawaty, Z.; Abdclwhab. E. M.; Hassan, M.K.; and Aly M.M.
...ty of backyards to avian influenza much easier than commercial poultry farms. Avian Influenza subtype H7 was isolated from wild migratory ducks in El-Abassa Lake at El-Sharkia Governorate in 2007. To avoid repeating the scenario of H5N1 introduction to Egypt through wild migratory ducks to Egyptian poultry sectors, this prompted us to survey avian influenza in Egyptian wetlands, especially...
Muhammad Akram1, Anirban Mandal2*, Mehwish Iqbal3, Arindam Mukherjee4, Ritika Bandyopadhyay5, Surendar Rangasamy6, Rida Zainab1, Muhammad Talha Khalil1, Pragnesh Parmar7 and Umme Laila1
...irus type 2 (HSV-2), and influenza A virus. Ascorbic acid, beta carotene and lots of phenolics play active parts in decreasing inflammation, postponing aging, and averting certain kinds of carcinomas. There are some phenolic compounds such as tannins, flavonoids, vitamins and lignins derived from plants acts as antioxidants. In this review article, we discussed upon history of use of plants as medicine, role of plants in viral infections, and anti-viral effect...
Mirwaise Khan1, Rubab Maqsood1, Hamad Bin Rashid2, Shakera Sadiq Gill1, Muhammad Noman1, Rafia Akram1, Muhammad Sajid Hasni1, Haroon Rashid3 and Mamoona Chaudhry1*
Iqra Munir*, Farrah Iftikhar, Hira Fatima, Sunbal Khalil Chaudhari and Roha Ramash
...re cough, asthma, fever, influenza, diabetes and liver diseases. 60% plants used to cure digestive diseases, diarrhea, cancer, inflammation, stomachache and dysentery. About 30% plants used to cure cardiovascular diseases and ulcers. The purpose of this study is to recognize wild plants gathered for medicinal purposes by the local residents of Mandi Bahauddin and to document the common names and therapeutic uses associated with these plants....
Abdel Satar Arafa
...>Abstract | Avian influenza virus (AIV) of the subtype H9N2 virus has been detected worldwide in wild and domestic birds as well as in few occasions in mammals. The virus was recorded in several African countries, with endemic status in Egypt and frequently reported in different other countries like Morocco, Tunisia, Uganda, Benin and Togo since 2016. Egypt and Morocco have applied a mass vaccination programme for commercial poultry to control the infec...
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