Rodolfo Villagra-Blanco1*, Gaby Dolz1, Danilo Montero-Caballero2, Juan Jose Romero-Zuniga2
...minants, and to identify serotypes present in the country....
Moustafa Kardjadj
...he disease. Currently, 3 serotypes of FMD virus (O, A and SAT-2) and 06 lineage are circulating in the North Africa, of which serotype O is responsible for most of the outbreaks. However, the rapid spread of SAT2 and other exotic FMDV lineages in Libya and Egypt demonstrated the need for a robust surveillance system to detect and respond effectively to exotic infections. Emergence and re-emergence of FMD virus ge...
John G. Bruno
...een the various captured serotypes, each of the anti-LPS aptamer candidates showed a preference for its cognate E. coli serotype. Results suggest that these aptamers may be useful for enriching specific E. coli pathogens during aptamer-magnetic separation in food samples, leading to a greater ability to detect these serotypes if coupled with genetic identification techniques such as PCR or gene probe hybridization....
Jehangir Khan1,2*, Inamullah Khan3, Ijaz Ali2, Aqib Iqbal2 and Muhammad Salman4
... cDNA of the four dengue serotypes. Six out of 35 (17.14%) adult mosquito pools were found positive for dengue virus with type-2 (77.77 %) and type-3 (11.11 %) while a single (11.11%) pool of concurrent infection with type 2 & 3 was also detected. The adult Ae. aegypti belonging to Shuba Bazaar showed 33% positivity for DENV with minimum infection rate (MIR) 11.1 while that of Wazir Bagh showed 16.67% positivity with MIR 5.5. Similarly, the Ae. a...
Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy
...gle infection. Different serotypes of E.coli and Salmonella could be recovered from dead birds however E.coli (O78) and S.enteritidis were recovered mainly from heart and liver. The recovered E.coli, S.aureus and P. haemolytica isolates recovered from mixed infection cases showed (57.1-100%) resistance to highly important antibiotic group (Doxycycline, Tetracycline, Trimethoprim sulfa methoxazole and Chloramphenicol) and showed dissimilar pattern of resistance...
Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1
...nsidered. Four different serotypes were identified with Salmonella Llandoff having the highest isolation rate in all the matrices sampled (58.8%), followed by, S. Kentucky (17.7%), S. Schwarzengrund and S. Havana had the lowest isolation rate (11.8%). S. Llandoff was isolated in all the matrices and was distributed across the three LBM whereas the other less frequent serovars had a more circumscribed distribution. Resistance to Methicillin, Penicillin, Erythro...
Muhammad Shahid1, Iram Amin1,*, Samia Afzal1, Zareen Fatima1, Sadia Zahid1, Usman Ashraf1 and Muhammad Idrees2
...bopictus having five serotypes 1-5 and infecting about 100 million people each year. To address dengue outbreak 2013 in Pakistan we aimed to conduct a comprehensive study at molecular level to determine the main causative serotype of 2013 DENV outbreak in Pakistan. Overall 703 serologically positive suspected patients from different major health centers of Pakistan were registered in present study of which 214 were females and 489 were male. Overall weight...
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*
...ult showed that the main serotypes of E. coli were O8, O64, O138, O157, O139 and O141, accounting for 60 (74.04%) of all strains, while mouse subcutaneous-infection model revealed that 45(55.56%) of the isolates were “killers”, 20 (24.69%) were pathopoiesia but not lead to die and 16 (19.75%) of the isolates were non virulent. This study reported the occurrence of pathogenic E. coli isolated from Tibetan piglets with white score diarr...
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
...rnaviridae. It has seven serotypes with no cross protection among these. FMD is responsible for heavy economic losses in cattle and buffalo. So rapid and accurate diagnosis of FMD is uttermost need of this time. Several lab.techniques are developed for rapid detection of FMD but these are expensive and time consuming. Elisa, cell culture, Reverse Transcriptase (RT-PCR) and real time PCR need specific high quality equipments in lab. Whereas Lamp PCR is simple a...
Naglaa M. Hagag1*, Mervat E. Hamdy1, Mary A. Sargious1, Sara M. Elnomrosy1, Nahed A. Ahmed1, Ayman A. Hamed1, Ahmed R. Habashi1, Essam I. Ibrahiem1, Mahmoud A. Abdel-Hakim2 and Momtaz A. Shahein1
Mumtaz Ali Khan1, Sher Bahadar Khan2, Shakoor Ahmad2, Irshad Ahmad3, Kashif Prince1*, Ghazunfar Rashid1, Mahboob Ali1, Imdad Ullah Khan4, Asad Ullah5, Naimat Ullah4, Muhammad Shoaib2 and Said Sajjad Ali Shah2
... infected with different serotypes (A, B, and C) were categorized into two groups healthy and diseased to compare the hematological and biochemical parameters. Hematobiochemical analysis indicated that mean erythrocyte count (RBC) and hemoglobin levels decreased while the mean leukocytes (WBC), platelets, packet cell volume (PCV) and total bilirubin levels increased significantly (P<0.05) in sheep infected with Clostridium perfringens type A. Sheep i...
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
...nd also copying specific serotypes O, A and SAT2 nucleotide sequence. In this work FMDV serotype O, A and SAT2 were isolated from cattle clinical samples (foot lesions). RNA extracted from clinical samples was subjected to reverse transcriptase polymerase chain reaction( Rt. PCR) nucleotide of FMDV ID and 3D genes were determined using standard automated sequencing technique. Phylogenetic analysis of FMD / SAT-2 /3D/Egypt/Sharkia/2013/ FMD / (KJ210080) reveale...
Fawzy Rania1, AboElkhair M.1, Bazid A.M.1, Sultan H.2, Hussien A.H.3
...ulation of different IBV serotypes in broiler sectors. Regular monitoring for evolution, epidemiology and genotyping of Egyptian IBV field strains is very important for screening the emergence of new variant strains as well as evaluating the existing vaccination programs....
Nashwa M. Helmy1 and Ahmed S. A.2
...animals. There are seven serotypes of FMD virus (FMDV), namely O, A, C, SAT 1, SAT
2, SAT 3 and Asia 1. Tests for antibodies of FMDV nonstructural proteins (NSPs) are
useful in providing evidence of previous or current viral replication in the host,
irrespective of vaccination status. NSPs, unlike structural proteins are highly conserved
and therefore are not serotype specific and as a consequence, the detection ...
Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3
...dies
against serotypes of FMDV (A),(O) and (SAT-2) by (SNT) as well as by analysis of negative
Non Structural Protein sera by solid phase competitive ELISA for differentiation between natural
infected and vaccinated animals by detection of Non-Structral protein of FMDV by priocheck
test. Five hundred sera were collected from vaccinated cattle and buffalo from five Egyptian
governorates (Two governorat...
Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1
.../014p with Massachusetts serotypes. The commercial H120 vaccine conferred partial protection against proventriculitis induced by Egypt/Qal/014p. Conclusion: IBV strains exhibiting proventriculitis were found co-circulating in broiler chickens in Egypt and optimum control of IBV in Egypt require preparation of vaccine from indigenous isolates beside periodic evaluation of cross-protective capabilities of such vaccine....
Dina A. Abdulrahman1, Ayman H. EL-Deeb2, Momtaz A. Shaheen1 & Hussein A. Hussein2
...of all seven
serotypes of FMDV. The positive-resulted samples were then tested using conventional PCR and pan
FMD primers. The same samples were tested again using serotype-specific primers to amplify the VP1
coding region and the products were sequenced for phylogenetic analysis.
Results: FMDV RNA was detected in eight out of the 12 samples tested using real time RT-PCR.
Conventional PCR revealed tha...
Eman H. Mahrous1, Mohamed. W. Abd Al -Azeem2, Faisal A. Wasel1, Waleed Younis2*
...olates belong to somatic serotypes 1, 3, and 12 with a percentage of 20%, 10%, and 10% respectively, while other isolates were untypable. All the recovered isolates were further subjected to multiplex PCR screening of some common virulence genes and revealed the presence of pfhA, hgbB, tbpA, toxA, sodA, ptfA, sodC, nanB, and oma87 with percentages of 30%, 60%, 10%, 100%, 70%, 90%, 90%, 90%, and 100% respectively. Antibiotic susceptibility tests of the recover...
Asmaa G. Mubarak1*, Mona M. Mustafa2, Mohamed W. Abdel-Azeem3, Dina N. Ali2
... of different Salmonella serotypes to hospitalized patients in Assiut Governorate, Egypt, as well as, to assess their pathogenic potential and antimicrobial resistance. Out of 150 chicken meals collected randomly from various restaurants and food shops including, shish-tawook, pane, and shawerma (50 for each), 10% were contaminated with Salmonella with the acquisition of shish-tawook (14%). On the other hand, 100 hand swabs that were assembled from food h...
Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*
...trol due to its multiple serotypes. The disease could be prevented by rapid diagnosis either molecular or serological test. However, the later test is inexpensive such as heamagglutination inhibition test (HI), but IBV fail to give Heamagglutination (HA) reaction without pretreatment. Therefore, we designed this study for preparation of IBV antigen by treating with different enzymes for HA reaction. IBV local isolates were characterized by SDS-PAGE and RT-PCR....
Sania Subhan Qureshi*,1, Baitullah Khan1, Shahid Khan1, Hanif Ur Rahman1 and Muhammad Subhan Qureshi2
...is caused by 7 different serotypes of FMD virus having different virulence. To compare the virulence of various FMD serotypes the study was designed to propagate the virus on BHK-21 cell line. The results revealed that serotype ‘A’ virus virulence is more as compared to serotype ‘O’ and ‘Asia-1’. Although less virulence difference was found between serotype ‘A’ and ‘O&rsq...
Gamilat A. Elsaid1, Weam Mohamed Baher1*, Eman Shukry1, Abeer E. Abd El. Ghafar2, Marwa Shalaby1
.... The identified E. coli serotypes were E. coli O2:H6, E. coli O26:H11, E. coli O55:H7, E. coli O78:H-, E. coli O111:H4, and E. coli O119: H. All isolated harbored 16S rRNA specific gene for E. coli. The expression of shiga toxin coding genes indicated that E. coli O2:H6, E. coli O111:H4, and E. coli O119:H harbored only stx2. E. coli O26:H11 and E. coli O55:H7 harbored both stx1, and stx2; however, E. coli O78:H- did not harbor any of the tested genes. The us...
Zohair S. Mulla1*, Mostafa M. Abdelhafeez2
...ther identified into six serotypes, namely E. coli O2:H6, O26:H11, O55:H7, O78:H-, O86:H11, and O119:H4. Detection of shiga toxin producing genes (stx1 and stx2) in the recovered serotypes was followed using multiplex PCR. A significant decrease in the microbial loads was recorded after dipping of the formulated ground beef as meat balls in the acid solutions, particularly at the acid cocktail 2%. To the best of our knowledg...
Eman H. Abotalp, Sahar R. Mohamed, Jakeen K. El Jakee
...ized with unsafe E. coli serotypes and the antibiotic resistance in these E. coli isolates. A total of 129 rectal swabs were gathered from apparent healthy and diarrheic canines and felines. By using Vitek2 compact system, 42 E. coli isolates (32.6%) were resulted from canines (24%) and felines (44.4%) rectal swabs. E. coli were serotyped to: O8, O25, O26, O28, O36, O55, O78, O86, O111, O114, O125, O127, O128 and O157. The resistance to 16 antibiotics and...
Hassan A. Aidaros, Eman M. Hafez, Halla E.K. El Bahgy*
...s against field isolated serotypes of E. coli, Pasteurella multocida, Campylobacter jejuni, and Staphylococcus aureus from chicken and duck farms production at a titer of 3×106 CFU (Colony Forming Unit) /cm2 in the absence and presence of organic matter (O.M). The results showed that the efficiency of disinfectants was significantly increase with high concentration and long contact time. The Prophyl 2000® was the most powerful disinfectant against fi...
H. A. Aidaros1*, S. S. Khalafallah1, M. S. Diab2, Nehal K. Alm Eldin2, Halla E.K. El Bahgy1
...70% & 60% of E. coli serotypes, respectively. The application of good biosecurity programs including strict measures in poultry farms is the preferable method to reduce the risk of pathogenic bacteria and reduce the use of antibiotics.
Keywords | Poultry farms, Hygiene, Enterobacteriaceae, Antimicrobial Resistance, Resistance gens.
...
Chunlan Shan1, Chaoying Liu1, Qin Lu1, Guowen Fu2, Syed Aftab Hussain Shah3, Rana Waseem Akhtar4, Ru Zhao2, Libo Gao2, Chang Liu2, Shushu Miao2, Hongdan Wang2 and Hong Gao2*
...of all isolated superior serotypes of E. coli was obtained. Five major high pathogenicity island (HPI) structural genes (irp1, irp2, irp3, irp4 and irp5) were cloned, sequenced and referenced with GenBank database. The sequence identities of irp1, irp2, irp3, irp4 and irp5 with GenBank were 98 %, 99 %, 99 %, 98 %, and 99 %, respectively. Forty-four E. coli isolates characterized similarity with enteroinvasive E. coli (EIEC), uropathogenic E. coli (UPEC), Y. pe...
Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3
...ng the recovered E. coli serotypes, the serotype O111:K58 (B4) was the most prevalent (38.2%). An inactivated polyvalent autogenous mastitis vaccine was prepared from the most encountered bacterial pathogens, namely, E. coli, S. aureus, Str. agalactiae and Str. dysgalactiae. The selected strains for vaccine preparation were inactivated with a predetermined minimal lethal dose of gamma radiation (4krad kGy/min) and the bacterial inactivation was assured using ...
Muhammad Mohsin Abbas1*, Sajjad Ur Rahman1, Muhammad Abubakar2, Muhammad Imran Arshad1 and Khurram Ashfaq3
...n of the prevalent virus serotypes in the area. The current cross-sectional study was conducted in the Bahawalpur district of Punjab, Pakistan, with the purpose to determine the prevalence of FMD virus serotypes Asia 1, O and A in bovines. A total of 838 bovine sera samples were tested through Solid phase competitive enzyme-linked immunosorbent assay (SPC-ELISA), based on structural proteins at the National Veterinary Labora...
Sar Zamin Khan1*, Muhammad Waqas1 and Sagar M. Goyal2
...uctive systems. Multiple serotypes of IBV have emerged due to the high rate of mutation and genetic recombination, which has made it difficult to control the disease. Certain serotypes have disappeared because of the availability and use of vaccines but new serotypes have emerged. A regular watch on the disease including virus variation, prevalence, pathogenesis and development of diagnost...
Aleena Kokab1, Ali Ahmad Sheikh1*, Masood Rabbani1, Wasim Shehzad2, Muhammad Ilyas Riaz1, Sohail Raza1 and Rida Haroon Durrani1
...y against non-Salmonella serotypes tested. Latent time period of 15, 15, and 20 min was documented for SEPL01, SEPL13, and SEPL20, respectively, with an average burst size of 110, 32, and 63 PFU CFU-1, respectively. All the three bacteriophages were tolerant of temperature range 4°C-70°C and pH between 3-12, thus providing a broader window of their application throughout the food chain. On chicken breast cuts, phage mix efficiently supported sustained ...
