Maureen McKeague1, Maria C. DeRosa2*
...egant new approaches and tools for SELEX. In this commentary, we discuss recent advancements in the selection of aptamers....
Nicola J. Stonehouse
...e be useful as molecular tools and also as novel therapeutic agents. This commentary describes RNA aptamers selected to bind to the RNA-dependent-RNA polymerase of foot-and-mouth disease virus. The aptamers are able to inhibit the function of the enzyme both in vitro and in the context of a sub-genomic replicon and highlight the future potential of this technology in many aspects of virus research as well as in diagnostics and therapeutics....
Faheem Khan*, Muhammad Zafarullah Khan
...loping special extension tools for those who cannot read....
Muhammad Nawaz Kandhro*, Habib-Ur-Rehman Memon, Muhammad Ali Ansari, Ahmed Naqi Shah
...d with the help of local tool spade. The statistical analysis of data showed that interculturing, Dual Gold, and sorghum and sunflower water extracts caused significant reduction of weeds and increased seedcotton yield as compared to weedy check. The combined application of sorghum @ 15 L ha-1+ Dual Gold @ 1.25 L ha-1 resulted in weeds mortality upto 66.6%, produced seedcotton yield of 3961.4 kg ha-1 and gave net benefit of Rs. 2,20,02...
Jonas Johansson Wensman1*a, Karl-Johan Leuchowius2,3 a, Jiting Yan1, Anna-Lena Berg4, Liv Bode5, Hanns Ludwig5, Sandor Belak6, Ulf Landegren2, Ola Soderberg2, Mikael Berg6
...n situ PLA), a selective tool for studying virus-host protein-protein interactions. BDV P (phosphoprotein) and N (nucleoprotein) have previously been reported to interact with several host proteins, thereby interfering with various signaling pathways. In this study, we focused on some of these interactions (BDV P-HMGB1, BDV N/P-Cdc2). First, we used rat glioma cell cultures persistently infected with a laboratory strain of BDV (C6BV ) to establish the assay. N...
Yongfeng Li, Mo Zhou, Xiao Wang, Libao Xie, Hua-Ji Qiu*
Anna-Maria Andersson*, Ann-Kristin Nyman, Per Wallgren
...LISA should be used as a tool for selection of breeding females in a control program for AD, the optimal sampling time at which the ADV antibody levels best can predict the breeding performance of the female needs to be further evaluated....
Ashley C. Banyard1* and Satya Parida2
...n by the OIE. Whilst the tools are available to attempt eradication there are a number of factors that will influence the strategy taken to progress with the aim of eradication. Here we describe current areas where knowledge improvement is required to shape our understanding of the disease and its distribution alongside areas of investigation that require consideration in any viral eradication campaign. ...
Klara Fischer1*, Erika Chenais1,2, Emeli Torsson1, Jonas Johansson Wensman1
...otential as an important tool in disease impact assessment and control. Particular attention is paid here to the potential role of PE in future research on the epidemiology and control of PPR. ...
Shahab e Saqib, Mokbul Morshed Ahmad, Sanaullah Panezai, Hidayatullah, Khalid Khan Khattak
...estionnaire was the main tool used to collect the data from farmers. The credit access ratio formula was employed that measured farmers’ access, in relative terms, to their landholdings. Likewise, credit adequacy formula was used to investigate farmers’ credit adequacy. A probit model was employed to find the important factors affecting access to credit. Results of access to credit ratio showed that small farmers had lower credit access to formal s...
Tarun Kumar Sharma, John G. Bruno and William C. Cho
...e emerged as competitive tools with the potential to replace antibodies in many diagnostic formats. The aptamer industry is continuously growing to create space in research and diagnostics markets. However, translation of aptamer in several applied disciplines is still lagging behind as compared to antibodies. In this article, we discuss the translational aspects of aptamers, current potential of aptamers and strategies to create the “bench-to-bedside&rd...
Jixue Hou1,2, Xueling Chen3, Jing Wang4, Hongwei Zhang2, Yu Xi2, Jie Xia2, Xinyu Peng2,* and Xiangwei Wu2,5*
...;">Mammals is a powerful tool to study pathophysiologic process of cardiovascular disease, but for a long time it is just limited to large animals, like pigs, rabbits, and big rats. The mice with reproduction fast cycle, lower costing, and close to human genes, can be showed about some phenomenon quickly, also more easy to form model of atherosclerosis. Thus, a mouse model of vascular injury is vitally important for researching the pathophysiological mechanism...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
...al studies are effective tools that could be used as complementary in diagnosis of amphistome infections in ruminants.
...
Muhammad Zafarullah Khan
...uipments and use of hand tools and implements. Most of the respondents have high competency level having practical farming experience. Study suggests pre-service along with in-service trainings along with higher degree in agriculture i.e. PhD for improving their technical competencies in agricultural mechanization...
Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*
...iplex-PCR is a sensitive tool for early and accurate diagnosis of Mycoplasma infections from samples collected from clinically sick and apparently healthy birds. In this way, current approach could be contributed well towards elimination and reduction of losses due to Mycoplasma infections in commercial poultry....
Usman Shakoor1*, Mudassar Rashid1, Abdul Saboor2, Nabila Khurshid1, Zuhair Husnain2 and Abdul Rehman2
...recipitation are helping tools to forecast scenarios for the climate in an area. Changes in these tools from the past due to any unavoidable circumstances for today and in the future is refers to climate change (CC). Besides other living being, crops are either benefited or adversely affected by these changes in the climate of an area. Maize is one of the widely grown crops in the world and even in Pakistan has also influenc...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1
...vel i.e. an ideal tool for marker-assisted selection of animals for future breeding programs.
...
Ayesha Zahid,Ammara Muazzam, Sidra Mustafa, Saba Irshad*,Malik Siddique Mahmood and Rehman Shahzad
...ipated by bioinformatics tools, which manifest mild changes in overall charge but altered post translational modifications in a way which might have a deleterious effect on ion channels anatomy and on the whole, pave ways to the genesis of cataract.
...
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
... on the use of molecular tools. In this study, we analysed the G-protein-coupled chemokine receptor (GPCR) genes of two LSDV isolates from Ixodid (hard) ticks (Amblyomma hebraeum) in Egypt. Multiple alignments of the nucleotide sequences revealed that both isolates had nine nucleotide mutations in comparison with the local reference strain, LSDV-Egypt/89 Ismalia. Compared with the GPCR sequences of SPV and GPV strains, 21 nucleotide insertion and 12 nucleotide...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1, Nisar Ahmad3, Ayesha Hassan1, Sultan Ayaz2, Sajid Umar4, Muhammad Luqman Sohail5,*, Muhammad Shafee6, Ikramullah Khan7, Mumtaz Ali Khan1, Azmat Ullah Khan8, Naimat Ullah Malik1 and Abdul Razzaq9
...t. For this purpose 360 stool samples were collected from the District Headquarter Hospitals of all the three districts and were screened through microscopy. Results yielded an overall prevalence of 11.11%, with significant difference (P<0.05) among different study areas. Prevalence was highest in district Bannu (11.66%), followed by district Lakki Marwat and Kohat with 10% in each district. Statistical analysis revealed significant difference (P<0.05) a...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
...entified. Bioinformatics tools were applied for construction of phylogenetic tree and genetic diversity analysis. Lower genetic diversity was observed. The finding of this research is prerequisite for future research.
...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,*
...c markers will be useful tools for investigating genetic diversity and population structure of this species.
... 
