Arshad Ali Khan1, Muhammad Sajid1, Abdur Rab1, Sahib Alam2, Abdul Bari3
...ay be used as an optimum fertilization dose for obtaining higher yield and improved fruit quality of tomato crop....
Malik Ikram Ullah1, Abdul Aziz Khakwani1*, Muhammad Sadiq1, Inayatullah Awan1, Muhammad Munir2, Ghazanfarullah1
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...s crucial for successful fertilization and embryonic development. Different techniques have been used to determine the integrity of DNA in which acridine orange (AO) assay is easy and less costly. Though used in many species but the variability exists in technique. Therefore, the present study was aimed to optimize the steps of AO staining protocol for buffalo sperm which has not been explored yet. Semen was collected from two mature buffalo bulls, diluted in ...
Abdul Malik1,2, Ghulam Abbas1,*, Abdul Ghaffar3, Sara Ferrando4, Lorenzo Gallus4 and Syed Sajjad A. Shah2,3
Zammurad Iqbal Ahmed, Muhammad Ansar*, Ashiq Saleem**, Zafar ullah Arif*, H.I. Javed and Rashid Saleem**
IMPROVEMENT OF MASH BEAN PRODUCTION UNDER RAINFED CONDITIONS BY RHIZOBIUM INOCULATION AND LOW RATES OF STARTER NITROGEN
...inoculation and nitrogen fertilization but inoculation alone was more effective by giving maximum economic yield. Various treatments exhibited significant differences for harvest index; however, maximum harvest index (22.04 %) was recorded in control plots. N in soil was increased by inoculation in -1 -1 combination with 40 and 50 kg N ha and 50 kg N ha alone, nevertheless, maximum -1 N in soil was recorded in inoculation + 50 kg N ha . ...
Doulat Baig*, Fida Mohammad Abbasi*, Habib Ahmed*, Maqsood Qamar** and Muhammad Ayub Khan**
RESPONSE OF SUNFLOWER HYBRIDS TO DIFFERENT NITROGEN LEVELS FOR PHYSIOLOGICAL AND AGRONOMICAL TRAITS UNDER FIELD CONDITIONS
...n the amount of nitrogen fertilization available in soil for the plant use. A two year study was conducted in 2012 and 2013 at National Agricultural Research Centre (NARC), Islamabad, Pakistan. The experiment was aimed to –1 –1 evaluate the effect of different nitrogen (N) levels (N = 0 kgha , N = 60 kgha , N = 0 1 2 –1 –1 –1 –1 80 kgha , N3 = 120 kgha , N4 = 180 kgha and N5 = 240 kgha ) on two sunflower hybrids, SMH-0907 an...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
...nt after in vitro fertilization. Sheep follicular fluid (SFF) was collected from different ovaries and supplemented to the in vitro maturation (IVM) media with proportions 0%, 10%, 20% and 40%. Oocyte maturation was assessed in different treatments and then cumulus-oocyte complexes were used for in vitro fertilization. Relative quantitative expression of mRNA transcripts related to apoptosis (Bax and Bcl...
Ali Sher1*, Khalid Naveed1, Gulzar Ahmad2, Ayub Khan1, Muhammad Saeed1 and Shah Masaud1
...th 150 kg N ha-1 over no fertilization thus combination of the 150 kg ha-1, 3 kg Zn ha-1 and 1 kg Fe ha-1 is recommended for improving biomass and leaf area. Further studies are recommended to understand the mechanistic approach of such improvement. ...
Munazza Yousra1*, Sair Sarwar1, Muhammad Mahmood Ul Hassan1, Muhammad Zameer khan1, Abdul khaliq2, Shahbaz Ahmad1 and Shamim Ul Sibtain Shah3
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
...99 (TCM199) for 24 h and fertilization (IVF) in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h, the presumptive zygotes were randomly distributed into 6 culture groups; Group I: Synthetic Oviductal Fluid (SOF) medium alone, Group II: SOF + co-culture, Group III: Conditioned SOF, Group IV: M199 alone, Group V: M199 + co-culture and Group VI: Conditioned M199 for in vitro culture (IVC). The percentage of embryos capable of crossing 8...
Muhammad Zameer Khan1, Sair Sarwar1*, Ahmad Khan1, Razaullah Khan1, Munazza Yousra1 and Muhammad Ilyas2
Rehman Ali Keerio1, Nighat Seema Soomro1, Aijaz Ahmed Soomro1, Mohammad Aquil Siddiqui2, Muhammad Tahir Khan2, Ghulam Shah Nizamani2*, Muhammad Nawaz Kandhro1, Maryum Siddiqui4, Hajra Khan3 and Farheen Deeba Soomro1
...te to optimizing current fertilization strategies and can help in improving sunflower production. ...
Quratulain1, Ata-ul-Mohsin1, Muhammad Naeem1, Ghulam Shabir1, Muhammad Khalid Rafique2 and Rashid Mahmood2*
Jaffar Hussain1*, Syed Farman Ali Shah1, M. Zeenat Ali1 and Javad Ahmad Shah2
...lue of Zn is low than Zn fertilization is recommended for better crop production. ...
Muhammad Mazhar Iqbal1*, Ijaz Ahmad2, Shahzad Maqsood Ahmed Basra3, Abu Baker Ijaz4, Zahid Hassan Tarar5, Muhammad Ansar6, Umer Iqbal7, Tayyaba Naz4, Bilquees Fatima8, Muhammad Akram9, Allah Wasaya10, Asif Iqbal11, Shakeel Ahmed Anwar12, Khalid Saif Ullah Khan13, Azeem Khalid14, Asif Aziz15 and Rashid Mehmood16
...g anthesis. Asynchronous fertilization and pollen desiccation reduce maize yield by reducing grain number and their size. Spray of hydrogen peroxide (H2O2), salicylic acid (SA) and ascorbic acid (AsA) may induce heat stress tolerance. Spray of SA, AsA, and H2O2 increased chlorophyll, relative water and nutrient contents, membrane stability index (MSI) and antioxidants activities in heat stress. Moreover, foliar application of chemicals during normal and late p...
Sri Nuryani Hidayah Utami*, Andin Muhammad Abduh, Eko Hanudin and Benito Heru Purwanto
Study on the NPK Uptake and Growth of Rice under Two different Cropping Systems with different Doses of Organic Fertilizer in the Imogiri Subdistrict, Yogyakarta Province, Indonesia
...is called Jarwo 2:1. The fertilization treatment for both rice cropping systems was the same, which is a mixture of cow manure and neem compost (1:1) at doses of 0, 3, 6, and 9 ton ha-1. The research data are presented in tables and graphs to show the effects of modification of the rice cropping system on the nutrient uptake. The results showed that the Jarwo 2:1 provided a higher N, P, K uptake compared to the Grid system. The provision of PFA had an impact o...
Amir Aziz1*, Mukkram Ali Tahir1, Noor-us-Sabah1, Ghulam Sarwar1 and Sher Muhammad2
Effect of Rice and Wheat Straw and K-Silicate Application on Maize Growth
...The benefits of silicate fertilization are often correlated with the amount of silicon uptake by plants. In order to evaluate the growth and yield behavior of maize crop in response to different source of Si (straw and potassium silicate), a pot experiment was conducted at College of Agriculture, University of Sargodha, Pakistan in 2017. Different treatments including T1= control, T2= wheat straw (deficient @ 200 mg kg-1 of soil), T3 = wheat straw (adequate @ ...
Ali Zohaib1*, Habib Ullah2, Shakeel Ahmad Anjum2, Tahira Tabassum2, Muzzammil Hussain1, Mohsin Nawaz3, Ghulam Abbas4 and Sohail Irshad5
Effect of NPK Fertilization Strategies on Growth, Yield, Nutrient Use Efficiency and Economic Benefits of Relay Intercropped Wheat in Cotton
...need for optimization of fertilization programs. This 2-years study was performed to investigate the influence of different strategies related to NPK fertilization timing viz. T1 (control, no NPK application), T2 (all PK at sowing, ½ N at tillering and ½ at booting), T3 (½ PK at sowing, ½ NPK at tillering and ½ N at booting), T4 (all PK at sowing, ½ N at sowing and ½ at tiller...
Muhammad Umair Hassan1, Muhammad Aamer1, Muhammad Nawaz2, Abdul Rehman3, Talha Aslam3, Ubaid Afzal4, Bilal Ahmad Shahzad3, Muhammad Ahsin Ayub5, Faryal Ahmed1, Ma Qiaoying1, Su Qitao1 and Huang Guoqin1*
Agronomic Bio-Fortification of Wheat to Combat Zinc Deficiency in Developing Countries
...nd grain Zn contents. Zn fertilization also maintains desirable Zn availability in soil solution and maintains the Zn pools in plant tissues during later stages thus resulting in an increase in Zn accumulation in wheat grains. Therefore, in this review, we discussed roles of Zn in plants and humans and possible strategies to combat Zn deficiency in humans. Additionally, challenges for agronomic and breeding strategies and possible benefits of both these strate...
Hillary M.O. Otieno
A Review of White Mango Scale (Aulacaspis tubercularis Newstead; Hemiptera: Diaspididae) in Sub-Saharan Africa: Distribution, Impact and Management Strategies
...rieties, spacing, better fertilization and shed management are also applied inappropriately. This review is therefore essential in providing the basis of identification of A. tubercularis. It also provides management strategies that could be adopted by mango farmers in the region. Last, it highlights key research themes worth prioritizing for better understanding and management of the pest in the region.
...
Sana Khan1*, Faiza Aman1, Muhammad Ismaeel2, Zafar Ali2, Mehboob Alam1, Sadeed Iqbal1 and Taimur Khan3
Muhammad Nawaz Kandhro1*, Nadia Mangrio1, Aijaz Ahmed Soomro1, Zia-ul-Hassan Shah2, Ghulam Sughra Mangrio3, Nihaluddin Mari4, Zulfiqar Ali Abbasi1 and Shazia Parveen Tunio5
Saleem Maseeh Bhatti1*, Muhammad Aslam Panhwar1, Zohaib ur Rehman Bughio1, Muhammad Saleem Sarki1, Allah Wadhayo Gandahi1, and Niaz Ahmed Wahocho2
... the impact of foliar Zn fertilization was quantified on growth, yield, and Zn concentration in strawberry (Fragaria x ananassa cv. Silder) at Nasarpur, Sindh, Pakistan during 2015-16. The foliar rates comprised of 33, 66, and 99 mg Zn L-1 (equivalent to 100, 200 and 300 mg ZnSO4 L-1) and distilled water (control treatment) and sprayed to strawberry plants before flower initiation. A continual increase in number of leaves, number and weight of berries, fresh w...
Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1
...has an important role in fertilization, embryonic survival and milk production traits in farm animals. The genomic characterization of STAT5A gene has not been assessed before in Nili Ravi Buffalo. In present research work, the sequence of the bovine STAT5A gene was analyzed to identify single nucleotide polymorphisms and its effect on evolutionary divergence. Nine polymorphisms, six in intronic and three in exonic regions, were identified in ...
Md. Alal Hossen1, Mohammad Amzad Hossain1*, A.K.M. Munzurul Hasan1,2, Bipresh Das1, Sohel Mian1, Mohammed Mahbub Iqbal1
...photomicroscope. Average fertilization and hatching rates varied from 74.33% to 83.89% and from 72.33% to 85.11% respectively among different treatments and control females did not ovulate. The eggs of M. gulio was firmly adhesive and immediately after spawning the average diameter of fertilized eggs were 0.48±0.01 mm. After fertilization, the first cleavage stage of M. gulio occurred within 40 min. The distinctivenes...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
...or more than 3 years but fertilization had not occurred. Separation of sperm samples from semen plasma, sperm counting and DNA extraction for assessment of clinical and molecular attributes was done using standard protocols. Healthy women were used as negative controls. This study revealed significant concentrations of Zn, Cd, Cr, Pb, Cu. Except Zn and Pb, the other 3 metals were found below the permissible limit set by WHO. Interestingly, the infertile males ...
Muhammad Jan1*, Muhammad Ashraf Sumrah1, Shoaib Akhtar1, Inam Ul Haq2, Muhammad Ramzan Anser1 and Iqra Yasmin1
...Olive (Olea europaea L.) fertilization considered as one of the important factor for production of olive fruit and oil. Keeping in view the modern fruit production physiology, nutrients serve as stimulants for cell development and fruit production. In Pakistan, Olive cultivation and production started from a previous one decade. Farmers have a lack of trend for fertilizer application to Olive orchards in a proper dose. Olive intensification for new orchard enh...
Muhammad Waryam Warraich1, Mukkram Ali Tahir1, Noor-us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Fakhar Mujeeb3, Muhammad Zeeshan Maznoor1, Aneela Riaz4 and Sarfraz Hussain2
... of artificial potassium fertilization the yield and supply potential is diminishing day by day. The cost of potassium fertilization is very high and awareness for external nutrition of potassium benefits is also on the minimum side. To overcome potassium bio-available losses from Pakistani farming system is very essential to sustain crop growth. Keeping in view of these issues, a pot study was performed at the departmental ...
Halidou Maiga Nafissatou1*, Abdou Moussa Mahaman Maâouia2, Moumouni Issa1, Marichatou Hamani1
...6.76 months). The age at fertilization of crossbred cows was 21.38 months compared to 32.57 months for Azawak cows. The calving interval was 17.85 ± 3.60 months and 18.50 ± 5.26 months for crossbred and Azawak cows, with no significant difference. It clearly appears that the crossbred cows grow better and start breeding earlier. These first results of the crossbreeding augur well for the future performance of milk and meat production of crossbred...
Saeed Akram Khan1, Shahid Sherzada1*, Muhammad Ashraf1, Durr-e-Shehwar1, Sonia Iqbal1, Usman Atique1,3, Farzana Abbas1, Sohail Ahmad2 and Muhammad Sultan Haider4
...maximum release of eggs, fertilization and hatching rates at 29 ºC. The maximum number of eggs were observed in 26oC whereas the lowest were obtained in 34oC. 85020.48). However, the temperature exceeding 30ºC not only increased the cell growth but significantly decreased the hatching time. On the contrary, temperature exceeding 32ºC caused denaturation of all eggs and larvae that resulted in reduced hatching rate. Microscopic examination reveal...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3*, Abd El-Nasser Ahmed Mohammed3
...pment upon ovulation and fertilization. Molecules transfer to oocytes through gap junctions of cumulus cells. Therefore, cumulus cells and their secretion, follicular fluid, effect on oocyte maturation and developmental potential of the resulting embryos in vivo and in vitro. Because of the importance of cumulus cells on follicular development, this review was made to collect and consolidate the current knowledge of cumulus cells effects on maturation of intac...
Muhammad Waqas Imam Malik1, Khalid Usman1*, Amir Hamza1, Muhammad Saad1, Said Ghulam2 and Azmat Ullah1
Waleed Asghar1,2*, Ahmad Mahmood1, Farhan Iftikhar2,3, Bushra Ahmad4, Rehmat Ullah5, Muhammad Bilal5, Abdul Latif6*, Muhammad Arsalan6, Madeeha Khan6, Rizwan Latif7 Muhammad Ehsan7
...additive to conventional fertilization could save chemical fertilizer’s contribution and minimize soil deterioration by overuse of chemical fertilizers....
Jiacheng Du and Yuping Cao*
...S) who underwent Invitro fertilization-embryo transfer (IVF-ET). This study selected 200 patients with PCOS who took IVF-ET in our hospital from January 2016 to January 2020 as subjects. The study subjects were divided into group A (low-level group, n=90) and group B (high-level group, n=110) according to serum AMH levels; and group C (successful group, n= 88) and group D (failure group, n=112) according to whether they completed clinical pregnancy. These grou...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3, Abd El-Nasser Ahmed Mohammed3, Al-Hassan Mohammed4*
...cyte meiotic maturation, fertilization and culture processes. Oocyte maturation is the first step for in vivo or in vitro embryo production. Oocyte maturation appears to be a simple step, but it is a very complex process. Maturation of oocytes include several changes at the cytoplasmic and nuclear levels that makes the oocytes able to undergo fertilization, pre-implantation and post-implantation embryo development. Oocyte me...
Abd El-Nasser Ahmed Mohammed*, Aiman Al Mufarji and Sultan Alawaid
...r successful maturation, fertilization and further embryonic development. The changes enable the zygotes to overcome maternal zygotic transition stage and follow their developmental competence to fetus. The changes were affected by in vivo and in vitro molecules and factors of the organisms and the surrounding conditions, respectively. Because of the importance of follicular changes during growth and development stages, which reflected in the developmental com...
Mustafa Erkan Özgür1*, Selim Erdogan2, Songül Aydemir3 and Hatice Yumusakbas2
...mprove semen quality and fertilization protocols....
Ghulam Jelani1, Qudratullah Kalwar1*, Asmatullah Kaka2, Abdullah Channo2, Ayyaz Ahmed1, Majid Hussain Soomro1, Hidayatullah Soomro1, Deepesh Kumar Bhuptani1 and Yar Muhammad Jalbani1
...d its transfer, in vitro fertilization, cryopreservation and transgenesis. Among these technologies, embryo transfer has achieved a great importance having produced numerous offspring from a genetically superior female. After the first successful transfer of mammalian embryos in 1890, it was approximately 60 years before significant progress became quite noticeable in the basic technology of embryo transfer in cattle. In embryo transfer process, embryo is coll...
I Nyoman Sulabda1, Anak Agung Gde Oka Dharmayudha2, I Wayan Nico Fajar Gunawan3, Dwi Fortuna Hashilonda4, Luh Gde Setyawati5, I Ketut Puja6*
...target of evaluating the fertilization capacity of frozen semen of Kintamani dogs using the artificial insemination method. Eight bitches were used in this experiment. The dog was in good health. The time of artificial insemination was performed 11 days after the onset of proestrus. Bitches are inseminated intravaginally. Pregnancy was confirmed by ultrasound 25 days post-AI. To our knowledge, this is the first successful case of AI in a Kintamani dog. From th...
Muhammad Hasnain1, Qudsia Nazir2, Ifra Saleem2*, Syed Shahid Hussain Shah1, Muhammad Asif Ali1 and Noor ul Ain3
...norance to micronutrient fertilization. Soils with less organic matter (OM), high in pH and calcareousness contribute in Zn deficiency. Moreover, efficiency of zinc (Zn) in soils is usually very little due to fixation of Zn in the soil matrix. So, a study was executed to find out the combined effects of urea coated with bio-augmented Zn 1% (Engro zabardast urea (EZU)-a fertilizer source of N and Zn) on rice growth, yield, and Zn bio- fortification. The applied...
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
...re oocytes mature before fertilization using semen from one bull, oocytes are fertilized then cultured for 48 hours. Embryo grouping based on cell count was conducted after 48 hours of culture and was recultured for 48 hours. Follicular fluid, oocyte maturation media and embryo culture media were collected using a disposable syringe and centrifuged, then supernatant in the collection, stored and frozen with -20 oC and growth factor concentrations were measured...
Sona Salem El-Nwehy1*, Assem Abbas Mohammed El-Naggar2 and Adel Badr El-Nasharty1
... recommended NPK mineral fertilization would affect vegetative growth, flower yield, quality, chemical constituents, and reduce mineral fertilization of NPK on Gladiolus hybrida cv. “Rose Supreme”. A pot experiment was undertaken during the planting seasons of 2020 and 2021 in the Antoniades Botanical Gardens, Horticulture Research Institute, Alexandria, Egypt. There are 7 treatments which consist of 3 percent of...
Abdul Aleem Memon1*, Inayatullah Rajpar2, Ghulam Murtaza Jamro2, Javaid Ahmed Shah3
...reous soil conditions, K fertilization either in the form of SoP or MoP could more effectively promote sunflower seedling growth....
Kenyum Lollen1, Judith Betsy C1*, Cheryl Antony2 and Stephen Sampath Kumar J3
...ts in terms of motility, fertilization and hatching rate....
Aziz Ahmed1*, Anila Naz Soomro2, Muhammad Ramzan Ali1, Muhammad Aleem Khan1 and Hasina Basharat1
...val, sustainability, and fertilization rates in Nile tilapia (Oreochromis niloticus). The experimental design was CRD with four treatments and three replicates. Lab prepared (Activation media A and B), one commercially available (Activation media C) and freshwater served as control. After the pre-developed broods were selected based on maturity approaches and collected sperm samples were diluted in the ratio of 1:30 fold media and divided into different aliquo...
Kalaiselvi Natarajan1, Karal Marx Karuppiah1, Sheena K Baby1, Sakthivel Mohammed2, Shanmugam Seerappalli Aran1 and Suresh Eswaran1*
...number of ovulated eggs, fertilization and hatching rate than ovaprim and ovatide. For dose optimization, the matured brooders were administered with synthetic hormone WOVA -FH at three different doses viz., 0.3, 0.5 and 0.7 and 1.0 ml per kg of body weight at triplicate basis. The highest number of ovulated eggs (3380.5 ±202.75), fertilization rate (90± 0.8) and hatching rate (88±0.88) were observed in ...
Julio Adolfo Corzo-Bacallao1*, Carlos Alfredo Salas-Macías1, Osvaldo Fonseca-Rodríguez2,3, Felipe R. Garcés-Fiallos1, Erika Isabel Alcívar-Muñoz1 and Henry Fabricio Baque-Loor1
...al weed control, without fertilization, irrigation, phytosanitary control, or shade regulation. In this scenario, and during an experimental period of 90 days (03/08/2022 - 26/10/2022), phenological variables of coffee trees maintained in a study area of 50 x 50 m at a high (S1: 51-70%) and low (S3: 1-30%) shade level was compared with those obtained at an intermediate shade level considered as standard (S2: 31-50%). The phenological variables related to veget...
Farrukh Hussain1, Sajid Aziz1, Gul Hassan2, Khalid Aziz1 and Sapna Raisham1
...till the pollination and fertilization of female flowers; and thereafter it is weeded out. There is need for extensively surveys and ecological analysis from more localities in the valley to get further information about the weed flora, their distribution, population size and possible losses due to these weeds. It is an established cash crop in the entire valley that can be respected for improving the socio-economic uplift of the area....
