Fusarium sp., an Endophytic Fungi Isolated from Sengon (Falcataria moluccana) Gall Rust, Shows Antimicrobial and Antioxidant Potential
Fusarium sp., an Endophytic Fungi Isolated from Sengon (Falcataria moluccana) Gall Rust, Shows Antimicrobial and Antioxidant Potential
Noor Rahmawati, Alfi Rumidatul* and Sopandi Sunarya
ABSTRACT
Many problems caused by antibiotic resistance encourage the exploration of new antibiotic compounds from natural materials, especially from endophytic fungal cultures. This study aims to evaluate different culture media that can support endophytic fungi to produce bioactive compounds that have antibacterial and antioxidant activity. The method used in this study was to ferment endophytic fungi from gall rust sengon in different media, namely PDB, PDB plus sengon wood powder, and PDB plus yeast, for 9 days, followed by extraction using ethyl acetate solvent and testing of antioxidant and antibacterial activity. The DPPH radical was used to measure antioxidant activity, and Bacillus subtillis was used as a representative for Gram-positive and Gram-negative For testing of antimicrobials, Escherichia coli and Pseudomonas aeruginosa were used. The experiment showed that the addition of yeast and sengon wood to PDB medium increased the growth and production of bioactive compounds extracted from fungal cultures. A fungal endophyte extract in ethyl acetate from all media used produced antioxidant compounds with high activity. Antibacterial activity was obtained from the extract of fungal cultures grown on PDB plus Sengon wood floor on day 6 for P. aeruginosa and E. coli, and day 3 for B. subtillis. In general, the three media used in this study encouraged endophytic fungi to produce antioxidant and antibacterial compounds.
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