Nematicidal activity of Citrullus colocynthis extracts against root-knot nematodes
Nematicidal activity of Citrullus colocynthis extracts against root-knot nematodes
T.S. Rizvi and F. Shahina†
ABSTRACT
The nematicidal activity of Citrullus colocynthis seed, pulp, fruit and creeper extracts were evaluated against rootknot
nematodes (Meloidogyne spp.) at 28 ± 2 C in laboratory. Gas chromatograph interfaced to a Mass
spectrometer (GC-MS) results indicated that C. colocynthis contains various bioactive compounds. The mentholic
extracts of seed had the highest mortality (100%) at highest exposure time (72 hrs) while, the pulp and fruit extracts
showed 60 and 80% mortality, respectively. Among various extracts of creeper hexane extract (HH-CC) showed
100% mortality after 72 hrs at all concentrations as compared with standard carbofuran. Whereas, HE-CC and HWCC
showed 100% mortality only at highest concentrations after 72 hrs. Ethyl acetate fraction was most active at
highest concentrations causing 60% nematode mortality after 72 hrs. However, aqueous fraction (1 and 0.5)
showed 40% mortality after 72 hrs exposure.
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June 2014
Pakistan Journal of Nematology, Vol. 32, Iss. 1, Pages 1-122
