Muhammad Ibrahim, Jawaria Ali Khan, Muhammad Sarwar Khan, Wasim Shehzad, Muhammad Avais, Ali Husnain, Naimatullah Khan, Muhammad Kamran Ameen and Abdullah Iqbal
...treatment of subclinical mastitis and to test out how these ingredients can be used for enhancement of milk quality as well as their effects on the haematological values. Total 30 cows were selected for this study kept in different dairy farms in the premises of Lahore. Out of these 30 cows, 24 had subclinical mastitis and 6 were healthy. Cows suffering from subclinical mastitis were divid...
Iqra Mahmood1, Asif Nadeem1*, Masroor Ellahi Babar2, Muhammad Muddassir Ali1, Maryam Javed1, Aisha Siddiqa1, Tanveer Hussain2 and Muhammad Tariq Pervez2
...ew strategies to prevent mastitis, a detailed understanding of the molecular mechanisms underlying the host immune response to the infectious agents is necessary. Identification of the genes, and their variants, involved in innate immune responses is essential for the understanding of this inflammatory disease and to identify potential genetic markers for resistance to mastitis. This article presents a systematic integration...
Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*
...occi are among the major mastitis causing organisms. Consumers of milk and milk products from mastitic animals are at high risk of foodborne infections. Escalating antibiotic resistance strived us to explore the alternate option to control mastitis. In ongoing era, bacteriophages are an alternate possible effective remedy. Therefore, in this study the infection ability of three lytic phages SA, SANF and SA2 was determined ag...
Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
...taphylococcus aureus mastitis infection, associated risk factors and subsequently their antimicrobial susceptibility.The milk samples were screened with surf field mastitis test and furthered to biochemical analysis. Pearson’ Chi-square test at nighty five percent confidence interval was used to analyze the collected data. Antibiotic sensitivity was checked with twenty four antibiotics by disc diffusion method. Ove...
Mahboob Ali1, Muhammad Avais1, Riaz Hussain2, Kashif Prince1,*, Muhammad Ijaz1, Mamoona Chaudhry 3, Sehrish Firyal4, Amjad Islam Aqib1, Naimat Ullah Khan5 , Mian Saeed Sarwar5, Hazrat Ali5, Shahid Hussain Farooqi1, Ikramul Haq1 and Muhammad Sajid Hasni3
...screened for subclinical mastitis using California mastitis test using convenient sampling technique. Biochemical examination was performed to isolate S. aureus that further were tested Penicillin, Glycopeptide, and Cephalosporin classes of antibiotics for confirmation of MDR isolates using disk diffusion method. MDR were tested in vitro for response against various antibiotics. A questionnaire was filled for e...
Kashif Prince1,*, M. Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Jawaria Ali Khan1, Nisar Ahmad3,Rais Ahmed2, Aamerzish Mushtaq4, Sajid Umar4 and Yung-Fu Chang5
...ated as a risk factor of mastitis and repeat breeding. About 48.43% cattle and 72.39% buffaloes were found positive in selenium deficiency. Species (cattle and buffaloes) were significantly (χ2=23.042, df=1, p<0.05) associated with selenium deficiency. The highest rates (90.62 %) of selenium deficient animals were observed in village Nathoki of tehsil Kasur. The risk factors such as Gender (χ2=11.31, df=1, p<0.05), feed comp...
A. N. Naqvi and K. Fatima*
INCIDENCE OF LIVESTOCK DISEASES IN NOMAL AND NALTAR VALLEYS GILGIT, PAKISTAN
...ve diseases, infections, mastitis, reproductive diseases, endoparasites, ectoparasites, wounds, hematuria, respiratory diseases, emaciation, hemorrhagic septic-emia, tumour, blue tongue, cow pox, enterotoxaemia, tetanus, paralysis and arthritis. In precise, endoparasites were found in 25.3% animals followed by respiratory diseases (24.74%). Most of the cattle (2053) and sheep (926) were found affected with endoparasites, whereas most of the goats (3960) were s...
Wazha Mugabe1,3,Gaolebale Segolame Mpapho1, John Kamau1, Wameotsile Mahabile2, Shalaulani James Nsoso1, Kealeboga Dipheko1 and Assar Ali Shah3,*
...ers were not informed on mastitis nor its management. From the 38 farmers informed on the diseases only, 76% were able to diagnose the disease. Most diagnosis was based on clinical observations (68) with the low utility of California Mastitis Test (CMT) (3%). Milk production was at subsistence scale and a secondary product from meat type breeds. With a commercial mindset, improved goat management and cooperative pooling and ...
Abid Hussain1,*, Mansur-ud-Din Ahmad2, Muhammad Hussan Mushtaq2, Mamoona Chaudhry2, Muhammad Sarwar Khan2, Michael Reichel3, Tanveer Hussain4, Amjad Khan2, MuhammadNisar2 and Imtiaz Ahmad Khan5
...clinical and subclinical mastitis in dairy buffaloes. The present study was conducted in two districts Lahore and Bhimber Azad Kashmir Pakistan. Total sample size for this survey was consisted of 1,036 lactating buffaloes. The screening of lactating buffaloes was performed with the California Mastitis Test. The overall prevalence of mastitis was recorded at 49%. Among
Aqeela Ashraf1,2,*, Muhammad Imran1 and Yung-Fu Chang2
.../i> isolates from bovine mastitis and identification of antimicrobial resistance and virulence genes associated with them. Antimicrobial susceptibility testing was performed by disk diffusion method. Resistance and virulence genes were detected by PCR. The results showed that 100% of isolates were resistant to penicillin, 54% to ampicillin, 44% to tetracycline and 30% to streptomycin, while none of them was resistant to chloramphenicol. These E. coli is...
Hasina Baloch1, Rahmatullah Rind2,*, Muhammad Rafique Rind3, Viram Kumar4, Nazia Baloch5 and Rajesh K. Oad6
...clinical and subclinical mastitis in buffaloes. Significantly higher prevalence (49. 07%) of clinical mastitis was observed in buffaloes with average age of 6 to 9 years whereas those of > 9 years of age showed higher (33.33%) but non-significant prevalence of subclinical mastitis. However, significant impact of the number of parity on the prevalence of clinical
Riffat Mehboob1*, Shahida Perveen2, Naseer Ahmed3
...BA), 12 of granulomatous mastitis (GM), 7 of lipoma, 5 of phylloides tumor (PL), 4 of fibrosis (F), 2 of intraductal papilloma (IDP) and 3 of accessory breast with Fibrocystic (FC) changes. The relation between the ages and frequency of the different types of lesions was also analyzed to understand the association between predisposing factors and the nature of lesions.
Conclusions: These results demonstrated that the FA is the most fre...
Amjad Islam Aqib1,*, Shagufta Nighat2, Rais Ahmed3, Saba Sana3, Muhammad Ameen Jamal4, Muhammad Fakhar-e-Alam Kulyar2 , Naimat Ullah Khan5, Mian Saeed Sarwar5, Muhammad Asif Hussain5, Asadullah5, Attaur Rahman5 and Sadeeq ur Rahman5,*
...incidence of subclinical mastitis (SCM), associated risk factors, involvement and antimicrobial susceptibility of Staphylococcus aureus in the development of SCM in District Faisalabad of Pakistan. For this purpose, a total of 384 goat milk samples were screened for SCM through surf field mastitis test (SFMT) and mastitis-positives cases were further investigated for isolation of
Hafiz Muhammad Umer Qaisar1, Tanveer Ahmad2, Muhammad Rizwan3*, Muhammad Saqib1
Antimicrobial efficacy of combination of lincomycin and spiramycin (Lispiracin™) as systemic dry cow therapy for controlling bovine mastitis
...) for controlling bovine mastitis through systemic dry period therapy,
present study was designed. A total of 20 dry pregnant cows were selected
randomly at the end of lactation and divided into two equal G1 and G2 groups.
Group G1 was treated with lincomycin@ 5mg/kg (IM) and spiramycin@ 10 mg/kg
(IM) (Inj. lispiracin®) at end of lactation and at 14th day pre calving while group G2
was kept as control. Samples of milk wer...
present study was designed. A total of 20 dry pregnant cows were selected
randomly at the end of lactation and divided into two equal G1 and G2 groups.
Group G1 was treated with lincomycin@ 5mg/kg (IM) and spiramycin@ 10 mg/kg
(IM) (Inj. lispiracin®) at end of lactation and at 14th day pre calving while group G2
was kept as control. Samples of milk wer...
Mahboob Ali1, Muhammad Avais1, Muhammad Ijaz1, Memoona Chaudhary2, Riaz Hussain3, Amjad Islam Aqib1, Naimat Ullah Khan4, Muhammad Luqman Sohail5,*, Madiha Khan6, Mumtaz Ali Khan1, Mehtab Ahmad7, Muhammad Sajid Hasni2, Imran Qaiser8, Ghazanfar Rashid1, Ikramul Haq1 and Ikramullah Khan8
... dynamics of subclinical mastitis, caused by Staphylococcus aureus, in dromedary camels (Camelus dromedarius) reared in two distinct agro-ecological zones of Pakistan viz. Cholistan desert and Suleiman mountain range. A total of 768 lactating she-camels were screened for subclinical mastitis using California mastitis test (CMT). A preformed questionnaire was used to ob...
Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1
...k factors of subclinical mastitis along with antibiotic resistance of one of the causative agent of bovine mastitis, Staphylococcus aureus. Ninety milk samples were collected from cows and buffaloes kept at different localities in district Kasur, Punjab, Pakistan. The possible risk factors inquired from farmers were animal type, breeds, urbanicity, age (years), teat washing, bedding area, lactating stage and previous ...
Abdul Kabir1, Laiba Uroog2*, Naushad Ahmad3, Fawad Ahmad3, Muhammad Saqib3, Noor Badshah4 and Taj Ali Khan3
...n: justify;">Subclinical mastitis is multifactorial inflammation of mammary glands in dairy animals, resulting in changes in milk quality, milk production, and economic losses to dairy farmers. It mainly occurs due to non-contagious environmental bacterial species. In Pakistan, it is the major disease of different dairy animals including bovines. However, only a little information is available about bacterial profile of the disease. A cross-sectional study wa...
Asghar Khan1,*, Aneela Zameer Durrani1, Arfan Yousaf2, Jawaria Ali Khan1, Mamoona Chaudhry1, Mumtaz Ali Khan3, Habibunnabi4 and Amjad Khan5
....
...
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
...ated to immunity against mastitis might be helpful for animal selection trait and prevention of this disease. The aim of the present study was to assess the effect of polymorphism in bovine tumor necrosis factor alpha (TNF-α) gene on immune function and its role in mastitis susceptibility in Pakistani Sahiwal cows. In this study, a total of 150 Sahiwal cows were selected suffering from clinical (n=50), subclinic...
Haq Aman Ullah1,*, Aneela Zameer Durrani2, Muhammad Ijaz2, Aqeel Javeed3, Muqadder Shah1 and Ikramul Haq1
...has role in sub-clinical mastitis and causes injurious effects on general health status of lactating goats.
...
Abdiaziz Idiris Mohamud1, Yonis Abukar Mohamed2, Osman Sheikh Ali Jama3, Pravin Mishra4* and Mohamed Idiris Mohamed5
Prevalence and Major Pathogens Associated with Clinical and Subclinical Mastitis in Dairy Camel (Camelus dromedarius) in Benadir Region of Somalia
...sectional study of camel mastitis was conducted on 150 lactating camels from July to December 2019 to figure out the prevalence of both clinical and subclinical mastitis in lactating camels in the Benadir Region of Somalia and also to recognize the associated microorganisms as causal agents of mastitis. The prevalence of this study was measured by using California ...
Muhammad Aamir Naseer1, Amjad Islam Aqib2*, Ambreen Ashar3, Muhammad Ijaz Saleem1, Muhammad Shoaib4, Muhammad Fakhar-e-Alam Kulyar1, Khurram Ashfaq1, Zeeshan Ahmad Bhutta1 and Shagufta Nighat5
...station, body condition, mastitis knowledge, treatment approach, and therapeutic drug use were significantly (p<0.05) associated with rise in S. aureus in dairy milk. All the tested isolates were found 100% resistant to Cefotaxime, Fusidic acid, and Ampicillin while 60-80% of these isolates were found sensitive to Cefoxitin, Gentamicin, Trimethoprim + Sulphamethoxazole, and Oxytetracycline. Except Trimethoprim + Sulphamethoxazole, non-signific...
Delower Hossain1,5, Shuvo Singha2,5, Chelsea Marie Van Thof3, Sanna Gough3 and AHM Musleh Uddin4,5*
Effect of Somatic Cells on Milk Quality and Human Health
...helps deciding efficient mastitis control strategies. Again, the safety margin evaluated though SCs ensures safe and healthy milk at consumer level devoid of bacterial presence in milk. This review highlights a brief overview of the importance and demand of estimating somatic cell count (SCC) and its effects on milk quality and human health....
Eman Khalifa1*, Riad Khalil2 and Haitham Elaadli3
...e the causative agent of mastitis in Egyptian Buffaloes and could be a potential risk for zoonotic transmission to man as well as economic losses. So, strict hygienic regulations and novel diagnostic tools should be used for prevention and detection of tuberculous mastitis in buffaloes’ farms. The general public health should also be intensely warned from consuming raw or unpasteurized milk. All these prophylactic meas...
Iqra Muzammil1, Muhammad Ijaz Saleem1, Amjad Islam Aqib2,*, Ambreen Ashar3, Syed Ashar Mahfooz1, Sajjad ur Rahman4, Muhammad Shoaib4, Muhammad Aamir Naseer1, Imran Khan Sohrani1, Javeed Ahmad1, Razaullah Saqi1, Fizzah Laeeq Lodhi1 and
Qaisar Tanveer5
...kistan. Using surf field mastitis test, collected milk samples were screened for subclinical mastitis at the spot for subsequent identification of pathogenic strains of S. aureus through microbiological examination in the laboratory. Non-probability statistical tools conferred 42% (84/200, CI=35.37-48.93) prevalence of subclinical mastitis, 38.1% S. aureus (32/84, CI=28.45-48...
Zhongyong Wu1, Wenzhu Li2, Chunxia Ni1, Zhuolin He1, Xiao Hou1 and Wanxia Pu1*
...n for the control of cow mastitis, to understand the genetic polymorphisms of dairy-sourced Staphylococcus aureus, we conducted genotyping of Staphylococcus aureus isolates from milk samples collected from cows with clinical mastitis using the random amplified polymorphic DNA (RAPD) method with three oligonucleotide primers. In total, 177 Staphylococcus aureus isolates were obtained from dairy farms in 5...
Waqar Ali1,3*, Muhammad Yaqoob2, Abida Arshad1, Guifu Dai3,Safir Ullah Khan1, Muhammad Arshad Malik4, Imran Ullah1 and Ihsan Ullah1
...clinical and subclinical mastitis through surf field mastitis test. The bacterial isolates identified through different biochemical test and colony morphology from positive milk sample were Staphylococcus aureus (25.4%), Escherichia coli (20.3%), Streptococcus (16.9%) in which (6.7%) Streptococcus agalactiae, and (10.1%) other streptococcus species, Pseudomonas aeruginosa (15.2%), Sal...
Roheela Yasmeen1*, Laiba Asif1 and Samia Djeffal2
...uring calving, lameness, mastitis, and swelling. The drug was also used to treat diverse problems such as dysmenorrhea, ocular inflammation, rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, and actinic keratosis etc. Although, it is good for livestock, its impact on the vulture population has been very deleterious. The present review discusses the chemical structure, uses, and the mechanism of the action of diclofenac and its negative impacts on v...
Md. Sanjid Hasan1, A K M Humayun Kober1, Eaftekhar Ahmed Rana2, Md Saiful Bari1,3,4*
...ller;">Keywords | Bovine mastitis, Causal agents, CMT, Prevalence, Risk factors ...
Sharjeel Saif1, Asghar Ali Kamboh1*, Ghulam Mustafa Solangi2, Rehana Burriro3, Hasina Baloch1, Atta Muhammad Memon2
...bovine animals including mastitis. The prevalence of mycoplasma mastitis is reported from many parts of the world in farm animals. The response of antimicrobials against this infection is very poor that usually lead to culling of infected animals. In Pakistan, unfortunately no data is available on the occurrence of mycoplasma mastitis in farm animals. Therefore, current field investigation...
Mohamed Abd El-Fattah Abo-Farw1, Maged Ahmed Aboul-Omran1, Abdel-Khalek El-Sayed Abdel-Khalek2*
...arly postpartum clinical mastitis on the reproductive performance, antioxidant, hormonal profile and trace elements in Egyptian buffaloes. A total of 90 multiparous lactating Egyptian buffaloes (500-600 kg live body weight, 5-7 years old, 3-5 parities, and 10.75 ± 2.66 kg/day milk yield were used in this study. The buffaloes were managed and feed at similar traditional conditions. Mastitis diagnosis was performed 21 d...
Muhammad F Tajol Ariffin1, Chai M Hian1, Muhammad Z Sukiman1, Mohd F Ghazali1, Siti M Zainal Ariffin2*
...ally induced subclinical mastitis in goats. Thirty lactating goats were divided into two groups (n=15 per group) challenged either by intramammary infusion of 1 x 103 cfu/mL Staphylococcus aureus (S. aureus) or phosphate-buffered saline (control). The haptoglobin (Hp), serum amyloid A (SAA) and α1-acid glycoprotein (AGP) levels in serum and milk were measured at pre-and post-infection (6, 24, 48 and 72 h) using the ELISA method. The SCC was determined by...
Sher Bahadar Khan1,*, Mumtaz Ali Khan2, Irshad Ahmad3, Faheem Ahmad Khan4, Hamayun Khan1 and Sher Ali Khan5
Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction
Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3
...n diagnosis of S. aureus mastitis through indirect Enzyme Linked Immunosorbent Assay (ELISA). For purification of fraction, bacterial fresh cultures were centrifuged at 3000 rpm / 20 min and were homogenate in phosphate buffer saline and centrifuged at 15000 rpm / 20 min that known as the crude antigen. It entered to affinity column chromatography [Cyanogen Bromide-Sepharose 4B (CNBr-Sepharose 4B)] (Sigma Chemical Co.), obtain the fraction. In brief, IgG of co...
Tariq Ali1,2*, Kamran1, Abdur Raziq1, Inam Ullah Wazir1, Anwar Ali1, Muhammad Ijaz Ali1, Muhammad Shuaib Khan2, Shakeeb Ullah2 and Sher Hayat Khan3
...he current prevalence of mastitis in lactating cattle, and buffaloes in Khyber Pakhtunkhwa during 2019-2020, to identify bacterial pathogens of mastitis, and to determine the antimicrobial resistance. A total of 1013 milk samples belonging to 726 cattle and 287 buffaloes were examined to detect clinical mastitis (CM) and subclinical mastitis (SCM). Masti...
Nurdan Urvaylioglu1*, Ogunc Meral1, Serkan Sayiner2, Ulvi Reha Fidanci1 and Arif Altintas1
...r diagnosing subclinical mastitis (SM) in dairy cows. Ten subclinical-, 16 clinical mastitis and 39 healthy Holstein cows were allocated to the study following California Mastitis Test (CMT). Thus, a total of 65 Holstein cows participated in the study. In the study, Staphylococcus aureus (n = 10) was isolated as the most dominant bacterial species seen in clinical and subclinical
Nurcan Karslioglu Kara*
... and diseases (lameness, mastitis, metritis and dystocia) in Simmental dairy cows bred under Turkey conditions. A total of 1110 Simmental cows (first calving to second calving) were assessed according to study criteria. While the relation between AFC and disease incidence and MY305 was not found significant, the relation between AFC and CI and DO was found significant and the optimum interval of AFC was determined 684≤ İBY≤730 d for minimum CI and DO. E...
Muhammad Said1, Amjad Hussain Mirani1, Abdul Kabir*1,2, Muhammad Haris Raza Farhan3, Abdul Latif Bhutto1, Ghulam Shabbir Barham1, Khaleeq Ur Rahman Bhutto4, Muhammad Uzair1, Maaz Khan1
...> ...
Muhammad Nadeem1, Muhammad Rizwan2,3*, Tanveer Ahmad4, Muhammad Kashif5, Aneela Zameer Durrani2, Muhammad Ali6, Asghar Abbas7, Safder Imran8, Sidra Saher9, Syeda Fakhra Waheed10
...as respiratory syndrome, mastitis, enterotoxaemia, tick and mite infestations and pox disease. There was no proof of rinderpest occurrence since long in area of examination. Participatory disease surveillance presented to be a worth full technique to obtain trustworthy data that may be helpful in making most effective policies for control and eradication of livestock diseases in and Pakistan.
Novelty Statement | Participato...
Nauman Zaheer Ghumman, Muhammad Ijaz*, Arslan Ahmed, Muhammad Umar Javed, Iqra Muzammil and Ahmed Raza
...A associated subclinical mastitis in 345 milk samples (Cattle n=173, Buffalo n=172) collected from indigenous bovines of district Rawalpindi, Pakistan. The milk samples were screened for S. aureus and the confirmed isolates were subjected to disc diffusion test, PCR, and SDS-PAGE analysis for the confirmation of methicillin resistance. The farm-associated and individual animal-associated risk factors were analyzed to check the association with S. aureus and MR...
Pakistan Journal of Zoology
February
Featuring
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MRSA Clinical Isolates Harboring mecC Gene Imply Zoonotic Transmission to Humans and Colonization by Biofilm Formation
Salman Hussain, Basit Zeshan, Rabiya Arshad, Saba Kabir and Naveed Ahmed
Pakistan J. Zool., Vol. 55, Iss. 2, pp. 999-1002
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Assessment of the Nutritive Value of Complete Feed Supplemented with Corn Stover and King Grass on Holstein Friesians Dairy Cattle
Ronny A.V. Tuturoong, Sony A. E. Moningkey and Nova L.I.M. Ogi
Pakistan J. Zool., Vol. 55, Iss. 2, pp. 995-998
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Effect of Exercise Intensity on Human Immune System under Different Environments
Yanjie Liu
Pakistan J. Zool., Vol. 55, Iss. 2, pp. 991-994
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Hematological Profile of Achai Cows during Different Stages of Pregnancy in Kohistan Hindukush Mountainous Range
Jawad Ahmad, Huang Yun-He, Arab Hussain, Amjadullah, Ahmad Yar, Subhanullah, Zeshan Ali, Hamid Sattar, Tariq Hussain, Hamayun Khan, Ali Gohar, Obaidullah, Nan Zhu Fang, Yanya Fang, Ihsan Ali and Zhongshu Li
Pakistan J. Zool., Vol. 55, Iss. 2, pp. 987-990
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