Addition of Sugarcane Juice to Tris Egg Yolk Buffer Diluent Improves the Quality of the Stored Diluted Semen of Kacang Goats at 5°C
Addition of Sugarcane Juice to Tris Egg Yolk Buffer Diluent Improves the Quality of the Stored Diluted Semen of Kacang Goats at 5°C
Lukmanhy1*, Enny Yuliani1, I Wayan Lanus Sumadiasa1, Lalu Ahmad Zaenuri1, Mardiansyah2
ABSTRACT
This study aimed to determine the effect of sugarcane juice addition to egg yolk tris-buffer diluent on the semen quality of Kacang goats at 5°C storage. Semen samples were collected twice a week from a 2-year-old Kacang goat using an artificial vagina. The study design consisted of three treatments; T0, T1, T2 and T3, consisting of 0%, 10%, 15%, and 20 % of sugarcane juice, respectively. The measured parameters included progressive motility, viability, and abnormalities of spermatozoa. The data obtained were analyzed using the analysis of variance (ANOVA) followed by Duncan’s multiple range test to determine the difference among the treatments. The current study showed that adding 15% sugarcane (T2) improved the semen parameters compared to the other treatments with 54% progressive motility, 74.8% viability, and 8.8% total abnormalities at 5°C for 4 days of storage. 15% sugarcane juice in egg yolk tris-buffer diluent preserves Kacang goat diluted semen at 5°C for 4 days.
Keywords | Egg yolk tris, Kacang goat, Semen quality, Sugarcane juice
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December 2023
Vol. 11, Iss. 12, pp. 1911-2081
