Analysis of the EPAS-1 Expression Level and Pathological Characteristics of Renal Cell Carcinoma
Analysis of the EPAS-1 Expression Level and Pathological Characteristics of Renal Cell Carcinoma
Lijiao Wang1, Haibin Chen2*, Hongyan Yu1, Zexian Fu3 and Jianjun Zhao2*
ABSTRACT
This study aims to analyse the expression level of the endothelial PAS domain protein 1 (EPAS-1) gene in renal cell carcinoma (RCC), adjacent tissue and metastatic lymph node tissue. For this study, 110 cases of RCC tissue and corresponding adjacent tissue and 40 cases of metastatic lymph node tissue were selected. Western blot and immunohistochemical methods were used to detect the expression of EPAS-1 in the three groups. The expression of EPAS-1 and the clinicopathology of RCC were further analysed. To examine the relationship between these characteristics, a receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of EPAS-1 expression for lymph node metastasis in RCC. Compared with the adjacent tissue, the expression of EPAS-1 in RCC was significantly increased (P < 0.05, χ2 = 4.311). The correlation between the expression of EPAS-1 and primary tumour staging, lymph node metastasis, distant metastasis and the International Society of Urological Pathology (ISUP) grade of clear cell RCC patients were analysed. The classification using ISUP was significantly correlated with the expression of EPAS-1 (P < 0.05). The ROC curve showed that the high expression of EPAS-1 has a certain diagnostic value for lymph node metastasis in RCC (P < 0.01). To conclude The high expression of EPAS-1 is closely related to the adverse clinicopathological characteristics of RCC and can be used as a potential therapeutic target for this disease. The detection of the EPAS-1 gene has an auxiliary diagnostic value for lymph node metastasis in RCC.
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