Detection of RHDV Antigen Using DAS ELISA Technique and the Influence of Breed, Sex, Age on the Incidence of RHDV Seroconversion in Asymptomatic Rabbits in West Java, Indonesia
Detection of RHDV Antigen Using DAS ELISA Technique and the Influence of Breed, Sex, Age on the Incidence of RHDV Seroconversion in Asymptomatic Rabbits in West Java, Indonesia
Retno Setyaningsih1, Sri Murtini2*, I Wayan Teguh Wibawan2, Surachmi Setiyaningsih2, Ekowati Handharyani3
ABSTRACT
The first case reported a seroprevalence study was conducted in the West Bandung District of West Java Province, Indonesia, revealed the presence of RHDV antibody in rabbit serums without clinical symptoms in some rabbit farms. The presence of antibodies to RHDV in rabbits without vaccination indicates exposure to the virus. Thus, this study aims to investigate the presence of RHDV antigen in asymptomatic seroconverted RHDV rabbits in West Bandung District, West Java Province, Indonesia also to see the relevance of seroconversion within rabbit’s profile. Liver, intestines, and faeces were collected purposively from seroconverted rabbits which showed no clinical signs of RHDV. Samples assayed with commercial ELISA Kit using DAS Technique. The informations (age, sex, breed) of antibody titers against RHDV from previous study is used for samples profiling. The results showed all samples negative for RHDV antigen with average value ±0.04 (positive cut off >0.334). Seroconversion of RHDV from all seven villages where most seroconversion are showed by samples from young rabbits compare to adult rabbits, female rabbits compare to male rabbits but insignificant among breeds group of Oryctolagus cuniculus species or European rabbits. There were no RHDV antigen can be found from asymptomatic seroconverted RHDV rabbits in this study using DAS ELISA while seroconversion was affected by age and sex while there was no significant difference of seroconversion among breeds of Oryctolagus cuniculus or European rabbits which spreading among asymptomatic rabbits in seven West Bandung Regency villages, Indonesia.
Keywords | Rabbit haemorrhagic disease, Seroconversion, Asymptomatic, DAS ELISA
To share on other social networks, click on any share button. What are these?
November 2023
Vol. 11, Iss. 11, pp. 1757-1910
