GC-MS ANALYSIS OF Sonchus asper ROOT EXTRACT FOR IDENTIFICATION OF FUNGICIDAL COMPOUNDS AGAINST Rhizoctonia solani
GC-MS ANALYSIS OF Sonchus asper ROOT EXTRACT FOR IDENTIFICATION OF FUNGICIDAL COMPOUNDS AGAINST Rhizoctonia solani
Muhammad Rafiq,1 Amna Shoaib,1 Arshad Javaid1
ABSTRACT
Black scurf disease incited by Rhizoctonia solani causes a heavy burden on the
potato crop due to occurrence of large number of persistent sclerotia on tubers, while the
current conventional practices against diseases are limited and are associated with toxicity
and resistance. Botanical fungicides have shown enormous antifungal potential against
many sclerotial forming phytopathogens. In the present study, methanolic root extract of
Sonchus asper was assessed for its antifungal activity against R. solani. The methanolic
extract was prepared by soaking dried root biomass in methanol and analyzed by GC-MS.
Antifungal potential of the extract was detected in test tubes filled with malt extract broth.
The results revealed fungal biomass was significantly reduced (52–97%) with increase in
concentrations of the extract (1.56–200 mg mL-1). GC-MS analysis revealed that benzoic
acid, 4-hydroxy-3-methoxy-, methyl ester; 13-cis-retinoic acid; pyridine, 2-pentyl-; 9-
octadecenamide, (Z)- and L-proline were important compounds responsible for the
antifungal activity. Hence, methanolic root extract might be a potential library for fungicidal
compounds against R. solani.
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September 2020
Vol. 26, Iss. 3
