Inhibition of Oxidative Stress and Inflammation by Fisetin Ameliorates Heat Stress-Induced Intestinal Injury in Rats
Kang Cheng1*, Zhihua Song2, Jingyi Niu1, Jin Huang1, Laiting Liu1, Jinrong Wang1 and Yong Zhang1*
1College of Biological Engineering, Henan University of Technology, No. 100 Lianhua Street, Zhengzhou 450001, Henan, People’s Republic of China
2School of International Education, Henan University of Technology, No. 100 Lianhua Street, Zhengzhou 450001, Henan, People’s Republic of China
* Corresponding author: chengkang68@126.com, yongzhang208@163.com
Fig. 1.
Structure of fisetin.
Fig. 2.
Effects of fisetin on the jejunal diamine oxidase (DAO) activity in heat-stressed rats. CON, rats were orally fed with 0.5% carboxymethylcellulose sodium; HS, rats were orally fed with 0.5% carboxymethylcellulose sodium and then subjected to heat treatment; HS-FIS, rats were orally fed with 100 mg FIS /kg body weight/day and then subjected to heat treatment. Results are expressed as mean and standard error (n=8). *P<0.05 was compared with the CON group; #P<0.05 was compared with the HS group.
Fig. 3.
Effects of fisetin on the genes expression related to jejunal injury markers (A), redox status (B) and inlammation (C) in heat-stressed rats. HSP70, heat shock protein 70; I-FABP, intestinal fatty acid-binding protein; MMP3, matrix metalloproteinase 3; GPX1, glutathione peroxidase 1; Nrf2, nuclear factor, erythroid 2-like 2; SOD1, superoxide dismutase 1; Keap1, Kelch-like ECH-associated protein 1; IL6, interleukin 6; IL10, interleukin 10; TLR4, toll-like receptor 4; TNF-α, tumor necrosis factor alpha; IFN-γ, interferon γ; CON, rats were orally fed with 0.5% carboxymethylcellulose sodium; HS, rats were orally fed with 0.5% carboxymethylcellulose sodium and then subjected to heat treatment; HS-FIS, rats were orally fed with 100 mg FIS /kg body weight/day and then subjected to heat treatment. Results are expressed as mean and standard error (n=8). *P<0.05 was compared with the CON group; #P<0.05 was compared with the HS group.
Fig. 4.
The jejunal histological appearance (hematoxylin and eosin). (A) CON, rats were orally fed with 0.5% carboxymethylcellulose sodium; (B) HS, rats were orally fed with 0.5% carboxymethylcellulose sodium and then subjected to heat treatment; (C) HS-FIS, rats were orally fed with 100 mg FIS /kg body weight/day and then subjected to heat treatment. Original magnification 100 ×, Scale bars = 100 μm.
Fig. 5.
Effects of fisetin on the jejunal tumor necrosis factor alpha (TNF-α) level (A) and myeloperoxidase (MPO) activity (B) in heat-stressed rats. CON, rats were orally fed with 0.5% carboxymethylcellulose sodium; HS, rats were orally fed with 0.5% carboxymethylcellulose sodium and then subjected to heat treatment; HS-FIS, rats were orally fed with 100 mg FIS /kg body weight/day and then subjected to heat treatment. Results are expressed as mean and standard error (n=6). *P<0.05 was compared with the CON group; #P<0.05 was compared with the HS group.