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Isolation, Identification and Biological Characteristics of Lactobacillus reuteri from Tibetan Pigs

Isolation, Identification and Biological Characteristics of Lactobacillus reuteri from Tibetan Pigs

Zhenyu Chang1,3, Qingqing Xiao1,2, Yourong Ye1,2, Mujahid Iqbal4, Mengqi Duan1,2, Yangzom Chamba1,2* and Peng Shang1,2*

1College of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, Tibet, People’s Republic of China
2The Provincial and Ministerial Co-founded Collaborative Innovation Center for R and D in Tibet Characteristic Agricultural and Animal Husbandry Resources
3Key Laboratory of Clinical Veterinary Medicine in Tibet, Tibet Agriculture and Animal Husbandry University, Linzhi, Tibet, People’s Republic of China
4Department of Pathology, Cholistan University of Veterinary and Animal Sciences (CUVAS), Bhawalpur-63100, Pakistan
 
Zhenyu Chang and Qingqing Xiao contributed equally to this work.
 
* Corresponding author: nemoshpmh@126.com, qbyz628@126.com

ABSTRACT

Lactic acid bacteria is an important dominant bacterial community in the intestinal tract, which can regulate the intestinal environment and maintain the balance of intestinal flora. However, there are few studies on Tibetan pig lactic acid bacteria. The purpose of this study was to isolate and identify lactic acid bacteria from Tibetan pig feces, and to provide experimental materials for the development and utilization of Tibetan pig probiotics. Seventeen strains of Lactobacillus isolated from Tibetan pig manure were identified by physiology, biochemistry, 16S rRNA molecular biology and acid and bile salt tolerance tests. The results showed that, screened strain is Lactobacillus Roy’s F1. Tibetan pig source Roy’s Lactobacillus F1 showed the ability to grow maximum when the inoculation was 1%, the temperature 37 ℃ and pH = 7. This strain depicted a strong tolerance for artificial gastric and intestinal juice. Roy’s Lactobacillus F1 can inhibit the growth of Staphylococcus aureus and Salmonella. Lactobacillus reuteri F1 also showed a strong hydrophobicity and self-agglutination ability. It is sensitive to butamicane, chloramphenicol, erythromycin, cefazolin, norfloxacin, cotrimoxazole and other antibiotics, and has resistance for ampicillin, gentamicin, ciprofloxacin, penicillin and so on. In this study, one strain of Tibetan Porcine Lactobacillus was screened, obtained, and its biological characteristics were found good, which could provide reference and support for the development and utilization of Tibetan porcine probiotics.

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Pakistan Journal of Zoology

August

Pakistan J. Zool., Vol. 56, Iss. 4, pp. 1501-2000

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