Molecular and Biological Characterization of Squash Leaf Curl Virus (SLCV) Affecting Common Beans in Egypt
Molecular and Biological Characterization of Squash Leaf Curl Virus (SLCV) Affecting Common Beans in Egypt
Amro A. Farrag1; Ahmed K. El-Attar1; Om-Hashem M. El-Banna2; Ibrahim A. I.2; H.
M. Mazyad1
ABSTRACT
Symptomatic leaf samples obtained from common bean plants were collected from
Qaliobeya governorate, Egypt, and tested for Squash Leaf Curl Virus (SLCV) infection by
PCR using both degenerate and specific oligonucleotide primers. SLCV (bean isolate) was
transmitted from naturally infected common bean onto twenty two species and varieties
belonging to six different families i.e. Moraceae, Solanaeae, Cucurbitaceae,
Leguminoceae, Chenopodiaceae, and Malvaceae using viruliferous whitefly (Bemisia
tabaci). Results revealed that SLCV could be transmitted to 16 out of 22 species and
positively back inoculated to beans from these hosts. Nucleotide sequencing of the
complete genome of the virus was as follow, DNA-A (2667 bp) and DNA-B (2621 bp).
The obtained sequences were submitted into the GenBank with accession numbers
KJ624994 and KJ579954.The full nucleotide sequence of DNA-A, DNA-B and all open
reading frames (ORF) of the SLCV-bean was aligned and compared with eleven different
isolates of the SLCV available in the GenBank. The phylogenetic analysis of the complete
nucleotide sequence revealed that SLCV is related to other isolates of SLCV from other
governorates in Egypt (Cairo and Ismailia) as well as isolates from Lebanon (SLCV-LB2),
Palestine (SLCV-Pal), Jordan (SLCV-JO) and Israel (SLCV-IL) sharing high identities
ranging from (90% to 97%).
To share on other social networks, click on any share button. What are these?
July 2014
Vol. 11, Iss. 2, Pages 1-310
