Preparation and Evaluation of Lumpy Skin Disease Hvperimmune Serum Coniugated with Fluorescein
Preparation and Evaluation of Lumpy Skin Disease Hvperimmune Serum Coniugated with Fluorescein
Abdel Razek,B.Omar*and Magda M.Sayed**
ABSTRACT
Hyperimmune serum against lumpy skin disease virus(LSDV) was prepared by inoculation of the virus in successive doses into susceptible two calves with complete and incomplete Freund’s adjuvant. Identity tests were applied by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT). Serum neutralization test (SNT) and ELISA tests were applied for titration and evaluation of the hyperimmune sera before conjugation with fluorescent. Total protein concentration of the prepared LSDV antisera was 0.8g/dl. Separation of anti-LSDV immunoglobulins 1gG were done using ammonium sulphate followed by conjugation with fluorescein isothiocyanate at pH 9.6. The anti-LSDV IgG conjugated fluorescein sterile and was used to detect LSDV in the MDBK cells and gave good results to dilution 1/20 while the reference conjugate to 1/30.
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